Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070961.t1 | XP_013469751.1 | 94.5 | 146 | 7 | 1 | 1 | 145 | 1 | 146 | 1.10E-65 | 259.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070961.t1 | P11432 | 70.1 | 157 | 34 | 2 | 1 | 145 | 1 | 156 | 9.6e-50 | 197.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070961.t1 | A0A072VP35 | 94.5 | 146 | 7 | 1 | 1 | 145 | 1 | 146 | 8.1e-66 | 259.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052469 | MS.gene070961 | 0.838793 | 2.33E-57 | -1.69E-46 |
| MS.gene055539 | MS.gene070961 | 0.815918 | 7.23E-52 | -1.69E-46 |
| MS.gene059717 | MS.gene070961 | 0.80948 | 1.85E-50 | -1.69E-46 |
| MS.gene061154 | MS.gene070961 | 0.800121 | 1.67E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070961.t1 | MTR_1g102830 | 96.800 | 125 | 4 | 0 | 21 | 145 | 22 | 146 | 8.45e-87 | 251 |
| MS.gene070961.t1 | MTR_1g102810 | 90.698 | 129 | 12 | 0 | 17 | 145 | 14 | 142 | 2.02e-83 | 243 |
| MS.gene070961.t1 | MTR_1g102780 | 76.978 | 139 | 18 | 1 | 21 | 145 | 22 | 160 | 3.42e-71 | 212 |
| MS.gene070961.t1 | MTR_1g102570 | 75.912 | 137 | 21 | 1 | 21 | 145 | 22 | 158 | 1.25e-69 | 208 |
| MS.gene070961.t1 | MTR_1g102800 | 75.735 | 136 | 21 | 1 | 22 | 145 | 22 | 157 | 3.46e-69 | 207 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070961.t1 | AT3G22840 | 43.125 | 160 | 71 | 4 | 1 | 145 | 1 | 155 | 2.26e-34 | 119 |
| MS.gene070961.t1 | AT4G14690 | 48.462 | 130 | 54 | 2 | 28 | 144 | 23 | 152 | 3.43e-32 | 113 |
Find 39 sgRNAs with CRISPR-Local
Find 63 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAGAAGGGATGTTGTTAAAT+TGG | 0.214729 | 1.4:-79519096 | None:intergenic |
| AACCTTCCGTTGAACCTTTC+AGG | 0.218116 | 1.4:-79519625 | None:intergenic |
| GTTGTTGACAGTTGCTTCTT+TGG | 0.263072 | 1.4:+79519756 | MS.gene070961:CDS |
| GCAGATAAGCACAAAGTTTA+CGG | 0.307578 | 1.4:+79519576 | MS.gene070961:intron |
| CACAAAGTTTACGGATTTGA+TGG | 0.311110 | 1.4:+79519585 | MS.gene070961:CDS |
| CCGTTGAACCTTTCAGGTGC+TGG | 0.322713 | 1.4:-79519619 | None:intergenic |
| TTTACGGATTTGATGGCTTT+TGG | 0.325653 | 1.4:+79519592 | MS.gene070961:CDS |
| GGTTTGTTCGATCAAATATC+TGG | 0.369233 | 1.4:+79519700 | MS.gene070961:CDS |
| GCAGGCTGCGGTGTCACCTT+TGG | 0.375655 | 1.4:-79519389 | None:intergenic |
| AACGGAAGGTTGGCCATGAT+TGG | 0.403068 | 1.4:+79519637 | MS.gene070961:CDS |
| TCTTTGGTTCCGTTATTTGA+AGG | 0.415473 | 1.4:+79519772 | MS.gene070961:CDS |
| TAGCTTGAAAGTTCGATCTA+TGG | 0.429335 | 1.4:+79519147 | MS.gene070961:CDS |
| TGCTGGTCATGGAGCTTGAC+AGG | 0.438975 | 1.4:-79519051 | None:intergenic |
| TTGTTAAATTGGTTAACTCT+AGG | 0.465217 | 1.4:-79519085 | None:intergenic |
| TAACTAACACCTTCAAATAA+CGG | 0.495001 | 1.4:-79519781 | None:intergenic |
| TGCAGATACAAAACCAATCA+TGG | 0.506046 | 1.4:-79519650 | None:intergenic |
| TCTAGGCATGCTAGAAATGC+TGG | 0.515845 | 1.4:-79519068 | None:intergenic |
| TTCGATCAAATATCTGGTGG+TGG | 0.522060 | 1.4:+79519706 | MS.gene070961:CDS |
| TTGGTGGGCCAGCACCTGAA+AGG | 0.525261 | 1.4:+79519611 | MS.gene070961:CDS |
| CTAGCATTCCTTCTGAAGCT+AGG | 0.532093 | 1.4:-79519127 | None:intergenic |
| CATGCTAGAAATGCTGGTCA+TGG | 0.550746 | 1.4:-79519062 | None:intergenic |
| CACTTGTATACAAGAACCAT+GGG | 0.556587 | 1.4:-79519732 | None:intergenic |
| CTCTACTACTCGTTCACCAA+AGG | 0.565802 | 1.4:+79519412 | MS.gene070961:CDS |
| GGTGAACGAGTAGTAGAGGC+AGG | 0.568710 | 1.4:-79519407 | None:intergenic |
| TGAAAGGTTCAACGGAAGGT+TGG | 0.570880 | 1.4:+79519627 | MS.gene070961:CDS |
| AAGCTAGGCATATACACAGA+AGG | 0.589450 | 1.4:-79519112 | None:intergenic |
| GGTGGAATTAGCAAAAGGGC+AGG | 0.590541 | 1.4:+79519678 | MS.gene070961:CDS |
| TTGTTCGATCAAATATCTGG+TGG | 0.594040 | 1.4:+79519703 | MS.gene070961:CDS |
| TGTATATGCCTAGCTTCAGA+AGG | 0.600911 | 1.4:+79519119 | MS.gene070961:CDS |
| CGAGTAGTAGAGGCAGGCTG+CGG | 0.601742 | 1.4:-79519401 | None:intergenic |
| CACCTGAAAGGTTCAACGGA+AGG | 0.608089 | 1.4:+79519623 | MS.gene070961:CDS |
| GTGGAATTAGCAAAAGGGCA+GGG | 0.608172 | 1.4:+79519679 | MS.gene070961:CDS |
| AGCTAGGCATATACACAGAA+GGG | 0.613758 | 1.4:-79519111 | None:intergenic |
| GAAAGTTCGATCTATGGCTG+AGG | 0.614830 | 1.4:+79519153 | MS.gene070961:CDS |
| ACACTTGTATACAAGAACCA+TGG | 0.618963 | 1.4:-79519733 | None:intergenic |
| CTTTGGTGAACGAGTAGTAG+AGG | 0.621298 | 1.4:-79519411 | None:intergenic |
| CCAGCACCTGAAAGGTTCAA+CGG | 0.629884 | 1.4:+79519619 | MS.gene070961:CDS |
| AGGGCAGAAAGAGCAACCAA+AGG | 0.630670 | 1.4:+79519373 | MS.gene070961:CDS |
| TATCTGGTGGTGGAATCCCA+TGG | 0.688884 | 1.4:+79519716 | MS.gene070961:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TATATATCAAACAAATATTA+TGG | + | chr1.4:79519187-79519206 | MS.gene070961:intron | 10.0% |
| !! | ATCATGATATCATTATATAA+TGG | + | chr1.4:79519554-79519573 | MS.gene070961:intron | 15.0% |
| !!! | GCATATTTAATTTCTATACA+TGG | + | chr1.4:79519233-79519252 | MS.gene070961:intron | 20.0% |
| ! | AATCATAAACGTGTTTGATA+TGG | - | chr1.4:79519267-79519286 | None:intergenic | 25.0% |
| ! | AATGTAAAGAGACATTGTTA+TGG | - | chr1.4:79519345-79519364 | None:intergenic | 25.0% |
| ! | AATGTCTCTTTACATTATTC+AGG | + | chr1.4:79519349-79519368 | MS.gene070961:intron | 25.0% |
| ! | TAACTAACACCTTCAAATAA+CGG | - | chr1.4:79519784-79519803 | None:intergenic | 25.0% |
| ! | TTGTTAAATTGGTTAACTCT+AGG | - | chr1.4:79519088-79519107 | None:intergenic | 25.0% |
| CTCTTTACATTATTCAGGAA+GGG | + | chr1.4:79519354-79519373 | MS.gene070961:intron | 30.0% | |
| TCTCTTTACATTATTCAGGA+AGG | + | chr1.4:79519353-79519372 | MS.gene070961:intron | 30.0% | |
| TTGCATGACTATAATCTTCT+TGG | - | chr1.4:79519486-79519505 | None:intergenic | 30.0% | |
| TTTATTACTATGCTCACCTT+TGG | - | chr1.4:79519431-79519450 | None:intergenic | 30.0% | |
| !! | GTCATGCAAAGTATTGATTT+CGG | + | chr1.4:79519497-79519516 | MS.gene070961:intron | 30.0% |
| !!! | ATTGGTTTTGTATCTGCAAT+GGG | + | chr1.4:79519655-79519674 | MS.gene070961:CDS | 30.0% |
| !!! | GTCGTTGATTTTGTAAATTC+CGG | + | chr1.4:79519314-79519333 | MS.gene070961:intron | 30.0% |
| ACACTTGTATACAAGAACCA+TGG | - | chr1.4:79519736-79519755 | None:intergenic | 35.0% | |
| AGACATTGTTATGGATTCTC+CGG | - | chr1.4:79519336-79519355 | None:intergenic | 35.0% | |
| ATCTATGGCTGAGGTAATTA+AGG | + | chr1.4:79519162-79519181 | MS.gene070961:intron | 35.0% | |
| CACAAAGTTTACGGATTTGA+TGG | + | chr1.4:79519585-79519604 | MS.gene070961:CDS | 35.0% | |
| CACTTGTATACAAGAACCAT+GGG | - | chr1.4:79519735-79519754 | None:intergenic | 35.0% | |
| CAGAAGGGATGTTGTTAAAT+TGG | - | chr1.4:79519099-79519118 | None:intergenic | 35.0% | |
| CATAAACGTGTTTGATATGG+TGG | - | chr1.4:79519264-79519283 | None:intergenic | 35.0% | |
| GCAGATAAGCACAAAGTTTA+CGG | + | chr1.4:79519576-79519595 | MS.gene070961:intron | 35.0% | |
| TAGCTTGAAAGTTCGATCTA+TGG | + | chr1.4:79519147-79519166 | MS.gene070961:CDS | 35.0% | |
| TGCAGATACAAAACCAATCA+TGG | - | chr1.4:79519653-79519672 | None:intergenic | 35.0% | |
| TTGTTCGATCAAATATCTGG+TGG | + | chr1.4:79519703-79519722 | MS.gene070961:CDS | 35.0% | |
| ! | GGTTTGTTCGATCAAATATC+TGG | + | chr1.4:79519700-79519719 | MS.gene070961:CDS | 35.0% |
| ! | TCTTTGGTTCCGTTATTTGA+AGG | + | chr1.4:79519772-79519791 | MS.gene070961:CDS | 35.0% |
| !! | TTTACGGATTTGATGGCTTT+TGG | + | chr1.4:79519592-79519611 | MS.gene070961:CDS | 35.0% |
| !!! | GATTGGTTTTGTATCTGCAA+TGG | + | chr1.4:79519654-79519673 | MS.gene070961:CDS | 35.0% |
| !!! | TTGGTTTTGTATCTGCAATG+GGG | + | chr1.4:79519656-79519675 | MS.gene070961:CDS | 35.0% |
| AAGCTAGGCATATACACAGA+AGG | - | chr1.4:79519115-79519134 | None:intergenic | 40.0% | |
| AGCTAGGCATATACACAGAA+GGG | - | chr1.4:79519114-79519133 | None:intergenic | 40.0% | |
| GTTGTTGACAGTTGCTTCTT+TGG | + | chr1.4:79519756-79519775 | MS.gene070961:CDS | 40.0% | |
| TGTATATGCCTAGCTTCAGA+AGG | + | chr1.4:79519119-79519138 | MS.gene070961:CDS | 40.0% | |
| TTCGATCAAATATCTGGTGG+TGG | + | chr1.4:79519706-79519725 | MS.gene070961:CDS | 40.0% | |
| !!! | TGGTTTTGTATCTGCAATGG+GGG | + | chr1.4:79519657-79519676 | MS.gene070961:CDS | 40.0% |
| AACCTTCCGTTGAACCTTTC+AGG | - | chr1.4:79519628-79519647 | None:intergenic | 45.0% | |
| ATGGGGGTGGAATTAGCAAA+AGG | + | chr1.4:79519673-79519692 | MS.gene070961:CDS | 45.0% | |
| CATGCTAGAAATGCTGGTCA+TGG | - | chr1.4:79519065-79519084 | None:intergenic | 45.0% | |
| CTCTACTACTCGTTCACCAA+AGG | + | chr1.4:79519412-79519431 | MS.gene070961:CDS | 45.0% | |
| CTTTGGTGAACGAGTAGTAG+AGG | - | chr1.4:79519414-79519433 | None:intergenic | 45.0% | |
| GAAAGTTCGATCTATGGCTG+AGG | + | chr1.4:79519153-79519172 | MS.gene070961:CDS | 45.0% | |
| GTGGAATTAGCAAAAGGGCA+GGG | + | chr1.4:79519679-79519698 | MS.gene070961:CDS | 45.0% | |
| TCTAGGCATGCTAGAAATGC+TGG | - | chr1.4:79519071-79519090 | None:intergenic | 45.0% | |
| TGAAAGGTTCAACGGAAGGT+TGG | + | chr1.4:79519627-79519646 | MS.gene070961:CDS | 45.0% | |
| TGGGGGTGGAATTAGCAAAA+GGG | + | chr1.4:79519674-79519693 | MS.gene070961:CDS | 45.0% | |
| ! | TTTTGTATCTGCAATGGGGG+TGG | + | chr1.4:79519660-79519679 | MS.gene070961:CDS | 45.0% |
| !! | ACGGATTTGATGGCTTTTGG+TGG | + | chr1.4:79519595-79519614 | MS.gene070961:CDS | 45.0% |
| !! | CGGATTTGATGGCTTTTGGT+GGG | + | chr1.4:79519596-79519615 | MS.gene070961:CDS | 45.0% |
| !! | CTAGCATTCCTTCTGAAGCT+AGG | - | chr1.4:79519130-79519149 | None:intergenic | 45.0% |
| AGGGCAGAAAGAGCAACCAA+AGG | + | chr1.4:79519373-79519392 | MS.gene070961:CDS | 50.0% | |
| CACCTGAAAGGTTCAACGGA+AGG | + | chr1.4:79519623-79519642 | MS.gene070961:CDS | 50.0% | |
| CCAGCACCTGAAAGGTTCAA+CGG | + | chr1.4:79519619-79519638 | MS.gene070961:CDS | 50.0% | |
| GGTGGAATTAGCAAAAGGGC+AGG | + | chr1.4:79519678-79519697 | MS.gene070961:CDS | 50.0% | |
| ! | AACGGAAGGTTGGCCATGAT+TGG | + | chr1.4:79519637-79519656 | MS.gene070961:CDS | 50.0% |
| !! | TATCTGGTGGTGGAATCCCA+TGG | + | chr1.4:79519716-79519735 | MS.gene070961:CDS | 50.0% |
| GGTGAACGAGTAGTAGAGGC+AGG | - | chr1.4:79519410-79519429 | None:intergenic | 55.0% | |
| TGCTGGTCATGGAGCTTGAC+AGG | - | chr1.4:79519054-79519073 | None:intergenic | 55.0% | |
| ! | CCGTTGAACCTTTCAGGTGC+TGG | - | chr1.4:79519622-79519641 | None:intergenic | 55.0% |
| CGAGTAGTAGAGGCAGGCTG+CGG | - | chr1.4:79519404-79519423 | None:intergenic | 60.0% | |
| ! | TTGGTGGGCCAGCACCTGAA+AGG | + | chr1.4:79519611-79519630 | MS.gene070961:CDS | 60.0% |
| !! | GCAGGCTGCGGTGTCACCTT+TGG | - | chr1.4:79519392-79519411 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 79519025 | 79519804 | 79519025 | ID=MS.gene070961 |
| chr1.4 | mRNA | 79519025 | 79519804 | 79519025 | ID=MS.gene070961.t1;Parent=MS.gene070961 |
| chr1.4 | exon | 79519025 | 79519174 | 79519025 | ID=MS.gene070961.t1.exon1;Parent=MS.gene070961.t1 |
| chr1.4 | CDS | 79519025 | 79519174 | 79519025 | ID=cds.MS.gene070961.t1;Parent=MS.gene070961.t1 |
| chr1.4 | exon | 79519371 | 79519433 | 79519371 | ID=MS.gene070961.t1.exon2;Parent=MS.gene070961.t1 |
| chr1.4 | CDS | 79519371 | 79519433 | 79519371 | ID=cds.MS.gene070961.t1;Parent=MS.gene070961.t1 |
| chr1.4 | exon | 79519580 | 79519804 | 79519580 | ID=MS.gene070961.t1.exon3;Parent=MS.gene070961.t1 |
| chr1.4 | CDS | 79519580 | 79519804 | 79519580 | ID=cds.MS.gene070961.t1;Parent=MS.gene070961.t1 |
| Gene Sequence |
| Protein sequence |
