Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070992.t1 | XP_003592388.2 | 95.1 | 245 | 11 | 1 | 1 | 245 | 1 | 244 | 7.30E-126 | 459.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070992.t1 | Q9FLI1 | 45.3 | 172 | 84 | 2 | 76 | 237 | 2 | 173 | 3.9e-27 | 123.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070992.t1 | G7ID49 | 95.1 | 245 | 11 | 1 | 1 | 245 | 1 | 244 | 5.3e-126 | 459.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene070992.t1 | TF | bHLH |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene070992 | MS.gene34423 | PPI |
| MS.gene070993 | MS.gene070992 | PPI |
| MS.gene070992 | MS.gene006365 | PPI |
| MS.gene070992 | MS.gene37667 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070992.t1 | MTR_1g102370 | 95.102 | 245 | 11 | 1 | 1 | 245 | 1 | 244 | 1.11e-171 | 473 |
| MS.gene070992.t1 | MTR_0007s0380 | 51.754 | 228 | 100 | 6 | 21 | 243 | 16 | 238 | 5.66e-64 | 199 |
| MS.gene070992.t1 | MTR_0007s0330 | 39.665 | 179 | 98 | 3 | 72 | 244 | 14 | 188 | 5.87e-33 | 119 |
| MS.gene070992.t1 | MTR_0007s0330 | 40.994 | 161 | 88 | 2 | 72 | 226 | 2 | 161 | 1.79e-31 | 114 |
| MS.gene070992.t1 | MTR_1g102380 | 35.714 | 182 | 101 | 2 | 61 | 231 | 4 | 180 | 4.30e-29 | 108 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070992.t1 | AT5G51780 | 46.328 | 177 | 79 | 5 | 74 | 237 | 23 | 196 | 9.58e-38 | 131 |
| MS.gene070992.t1 | AT5G51780 | 46.286 | 175 | 78 | 5 | 76 | 237 | 2 | 173 | 2.92e-37 | 129 |
| MS.gene070992.t1 | AT4G25400 | 44.444 | 171 | 88 | 3 | 70 | 237 | 19 | 185 | 7.01e-36 | 126 |
| MS.gene070992.t1 | AT4G25400 | 46.405 | 153 | 75 | 3 | 70 | 219 | 19 | 167 | 3.29e-33 | 119 |
| MS.gene070992.t1 | AT4G25410 | 41.818 | 165 | 86 | 3 | 80 | 234 | 47 | 211 | 2.12e-32 | 118 |
| MS.gene070992.t1 | AT5G51790 | 38.919 | 185 | 101 | 3 | 62 | 234 | 9 | 193 | 3.06e-31 | 114 |
| MS.gene070992.t1 | AT5G51790 | 38.919 | 185 | 101 | 3 | 62 | 234 | 74 | 258 | 1.24e-30 | 114 |
| MS.gene070992.t1 | AT5G51790 | 40.000 | 170 | 90 | 3 | 62 | 219 | 9 | 178 | 1.09e-29 | 110 |
| MS.gene070992.t1 | AT5G51790 | 40.000 | 170 | 90 | 3 | 62 | 219 | 74 | 243 | 3.84e-29 | 110 |
| MS.gene070992.t1 | AT1G62975 | 32.161 | 199 | 111 | 3 | 63 | 237 | 61 | 259 | 6.02e-24 | 97.1 |
| MS.gene070992.t1 | AT1G12540 | 35.196 | 179 | 101 | 5 | 76 | 240 | 75 | 252 | 3.45e-22 | 92.4 |
| MS.gene070992.t1 | AT1G62975 | 33.149 | 181 | 97 | 3 | 63 | 219 | 61 | 241 | 1.72e-21 | 90.1 |
Find 43 sgRNAs with CRISPR-Local
Find 58 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGCCCCACTTTCTAAATTTC+TGG | 0.103688 | 1.4:+79164936 | MS.gene070992:CDS |
| GAAGAGGGAGAAGGGATCTA+AGG | 0.225427 | 1.4:-79164592 | None:intergenic |
| CTGGAACAACTGATGAAATA+TGG | 0.304748 | 1.4:+79164955 | MS.gene070992:CDS |
| TATTGAAGATAAGAAGAAAA+TGG | 0.318828 | 1.4:+79164514 | MS.gene070992:CDS |
| TCTATATAAAATAATTTGTT+TGG | 0.336326 | 1.4:-79164461 | None:intergenic |
| ATAAGAAGAAAATGGTTCAT+AGG | 0.350975 | 1.4:+79164522 | MS.gene070992:CDS |
| ACTAAATGATGGATTCATAT+TGG | 0.351963 | 1.4:-79165195 | None:intergenic |
| AAATCACTTGAAGAAGAATA+TGG | 0.357120 | 1.4:+79164762 | MS.gene070992:CDS |
| TATATAGATCGTACAAATCA+TGG | 0.422086 | 1.4:+79164476 | MS.gene070992:CDS |
| GTGGTTGAGACTATTAGATA+AGG | 0.433605 | 1.4:-79164339 | None:intergenic |
| TGGAAGAACTAAGTTACAAA+AGG | 0.434554 | 1.4:+79164782 | MS.gene070992:CDS |
| TACCAGTACTTGCTTTATAG+AGG | 0.437746 | 1.4:+79164909 | MS.gene070992:CDS |
| ATACCTCACACTGCACACTA+TGG | 0.439476 | 1.4:-79165026 | None:intergenic |
| CCACAGTTCTATTATTCTTG+TGG | 0.455790 | 1.4:-79164873 | None:intergenic |
| ATATAAAATAATTTGTTTGG+TGG | 0.460635 | 1.4:-79164458 | None:intergenic |
| AAGAGGGAGAAGGGATCTAA+GGG | 0.467872 | 1.4:-79164591 | None:intergenic |
| TAATCTGAACAACTAAATGA+TGG | 0.468144 | 1.4:-79165206 | None:intergenic |
| TAAGAAGAAAATGGTTCATA+GGG | 0.475156 | 1.4:+79164523 | MS.gene070992:CDS |
| TAGATCTAACTGAGCTAAGA+AGG | 0.478104 | 1.4:+79165165 | MS.gene070992:CDS |
| TTTACAACGATGCAATGAAC+TGG | 0.483997 | 1.4:+79164310 | MS.gene070992:CDS |
| AGTTCATTGCATCGTTGTAA+AGG | 0.484172 | 1.4:-79164308 | None:intergenic |
| AAAATAATTTGTTTGGTGGC+CGG | 0.487549 | 1.4:-79164454 | None:intergenic |
| TGGTTGAGACTATTAGATAA+GGG | 0.494385 | 1.4:-79164338 | None:intergenic |
| TTCCTCTATAAAGCAAGTAC+TGG | 0.516936 | 1.4:-79164911 | None:intergenic |
| GTTCCAGAAATTTAGAAAGT+GGG | 0.518377 | 1.4:-79164939 | None:intergenic |
| TTTATCTGATCAAATGAATG+AGG | 0.525114 | 1.4:+79164726 | MS.gene070992:CDS |
| TGTTCCAGAAATTTAGAAAG+TGG | 0.525663 | 1.4:-79164940 | None:intergenic |
| CACAAGAATAATAGAACTGT+GGG | 0.526811 | 1.4:+79164874 | MS.gene070992:CDS |
| GGAAGAACTAAGTTACAAAA+GGG | 0.531740 | 1.4:+79164783 | MS.gene070992:CDS |
| GAAGCAAAGGAGACAAGAAA+TGG | 0.533586 | 1.4:+79164553 | MS.gene070992:CDS |
| TCTCCCTCTTCACTTCATCA+AGG | 0.533778 | 1.4:+79164604 | MS.gene070992:CDS |
| TCACCTTGATGAAGTGAAGA+GGG | 0.538188 | 1.4:-79164607 | None:intergenic |
| GATGAAGTGAAGAGGGAGAA+GGG | 0.546212 | 1.4:-79164600 | None:intergenic |
| CCCACAACATCACAAAATCT+CGG | 0.553306 | 1.4:+79164364 | MS.gene070992:CDS |
| AGTACTTGCTTTATAGAGGA+AGG | 0.560200 | 1.4:+79164913 | MS.gene070992:CDS |
| TTCCAGAAATTTAGAAAGTG+GGG | 0.570258 | 1.4:-79164938 | None:intergenic |
| ATAGGGAAATTGAGAAGCAA+AGG | 0.575199 | 1.4:+79164540 | MS.gene070992:CDS |
| TGATGAAGTGAAGAGGGAGA+AGG | 0.577558 | 1.4:-79164601 | None:intergenic |
| CTCAGTTAGATCTACACTGT+CGG | 0.580625 | 1.4:-79165156 | None:intergenic |
| TTCACCTTGATGAAGTGAAG+AGG | 0.603402 | 1.4:-79164608 | None:intergenic |
| CCACAAGAATAATAGAACTG+TGG | 0.617517 | 1.4:+79164873 | MS.gene070992:CDS |
| ACTCCATAGTGTGCAGTGTG+AGG | 0.655288 | 1.4:+79165023 | MS.gene070992:CDS |
| TAGTGAAACTACAAGAAACA+TGG | 0.661078 | 1.4:-79164849 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TCTATATAAAATAATTTGTT+TGG | - | chr1.4:79164464-79164483 | None:intergenic | 10.0% |
| !!! | ATATAAAATAATTTGTTTGG+TGG | - | chr1.4:79164461-79164480 | None:intergenic | 15.0% |
| !!! | TGATTTTTCTCTTAAAAAAA+TGG | + | chr1.4:79164424-79164443 | MS.gene070992:CDS | 15.0% |
| !!! | TTTAATCATTAAAGTTTTCT+AGG | + | chr1.4:79164646-79164665 | MS.gene070992:intron | 15.0% |
| !! | TATCATCTGTTATAACTTTA+GGG | + | chr1.4:79164670-79164689 | MS.gene070992:intron | 20.0% |
| !! | TATTGAAGATAAGAAGAAAA+TGG | + | chr1.4:79164514-79164533 | MS.gene070992:CDS | 20.0% |
| !! | TTAAAAAAATGGATACTAAC+CGG | + | chr1.4:79164435-79164454 | MS.gene070992:CDS | 20.0% |
| !! | TTATCATCTGTTATAACTTT+AGG | + | chr1.4:79164669-79164688 | MS.gene070992:intron | 20.0% |
| !!! | AAACATGGCTTTTATTTTTA+AGG | - | chr1.4:79164837-79164856 | None:intergenic | 20.0% |
| !!! | TATTTATTGTTTCTCTAACA+GGG | + | chr1.4:79164694-79164713 | MS.gene070992:intron | 20.0% |
| !!! | TTATTTATTGTTTCTCTAAC+AGG | + | chr1.4:79164693-79164712 | MS.gene070992:intron | 20.0% |
| ! | AAATCACTTGAAGAAGAATA+TGG | + | chr1.4:79164762-79164781 | MS.gene070992:CDS | 25.0% |
| ! | ATAAGAAGAAAATGGTTCAT+AGG | + | chr1.4:79164522-79164541 | MS.gene070992:CDS | 25.0% |
| ! | TAAGAAGAAAATGGTTCATA+GGG | + | chr1.4:79164523-79164542 | MS.gene070992:CDS | 25.0% |
| ! | TAATCTGAACAACTAAATGA+TGG | - | chr1.4:79165209-79165228 | None:intergenic | 25.0% |
| ! | TATATAGATCGTACAAATCA+TGG | + | chr1.4:79164476-79164495 | MS.gene070992:CDS | 25.0% |
| ! | TTTATCTGATCAAATGAATG+AGG | + | chr1.4:79164726-79164745 | MS.gene070992:CDS | 25.0% |
| !!! | ACTAAATGATGGATTCATAT+TGG | - | chr1.4:79165198-79165217 | None:intergenic | 25.0% |
| CACAAGAATAATAGAACTGT+GGG | + | chr1.4:79164874-79164893 | MS.gene070992:CDS | 30.0% | |
| GGAAGAACTAAGTTACAAAA+GGG | + | chr1.4:79164783-79164802 | MS.gene070992:CDS | 30.0% | |
| GTTCCAGAAATTTAGAAAGT+GGG | - | chr1.4:79164942-79164961 | None:intergenic | 30.0% | |
| TAGTGAAACTACAAGAAACA+TGG | - | chr1.4:79164852-79164871 | None:intergenic | 30.0% | |
| TGGAAGAACTAAGTTACAAA+AGG | + | chr1.4:79164782-79164801 | MS.gene070992:CDS | 30.0% | |
| TGGTTGAGACTATTAGATAA+GGG | - | chr1.4:79164341-79164360 | None:intergenic | 30.0% | |
| TGTTCCAGAAATTTAGAAAG+TGG | - | chr1.4:79164943-79164962 | None:intergenic | 30.0% | |
| TTCCAGAAATTTAGAAAGTG+GGG | - | chr1.4:79164941-79164960 | None:intergenic | 30.0% | |
| ! | TGCTTTTCTATTCAAGTCAA+TGG | + | chr1.4:79164994-79165013 | MS.gene070992:CDS | 30.0% |
| !! | AAAATAATTTGTTTGGTGGC+CGG | - | chr1.4:79164457-79164476 | None:intergenic | 30.0% |
| !!! | TGGCTTTTATTTTTAAGGCT+AGG | - | chr1.4:79164832-79164851 | None:intergenic | 30.0% |
| AGTACTTGCTTTATAGAGGA+AGG | + | chr1.4:79164913-79164932 | MS.gene070992:CDS | 35.0% | |
| AGTTCATTGCATCGTTGTAA+AGG | - | chr1.4:79164311-79164330 | None:intergenic | 35.0% | |
| ATAGGGAAATTGAGAAGCAA+AGG | + | chr1.4:79164540-79164559 | MS.gene070992:CDS | 35.0% | |
| CCACAAGAATAATAGAACTG+TGG | + | chr1.4:79164873-79164892 | MS.gene070992:CDS | 35.0% | |
| CCACAGTTCTATTATTCTTG+TGG | - | chr1.4:79164876-79164895 | None:intergenic | 35.0% | |
| CTGGAACAACTGATGAAATA+TGG | + | chr1.4:79164955-79164974 | MS.gene070992:CDS | 35.0% | |
| GTGGTTGAGACTATTAGATA+AGG | - | chr1.4:79164342-79164361 | None:intergenic | 35.0% | |
| TACCAGTACTTGCTTTATAG+AGG | + | chr1.4:79164909-79164928 | MS.gene070992:CDS | 35.0% | |
| TAGATCTAACTGAGCTAAGA+AGG | + | chr1.4:79165165-79165184 | MS.gene070992:CDS | 35.0% | |
| TTCCTCTATAAAGCAAGTAC+TGG | - | chr1.4:79164914-79164933 | None:intergenic | 35.0% | |
| TTTACAACGATGCAATGAAC+TGG | + | chr1.4:79164310-79164329 | MS.gene070992:CDS | 35.0% | |
| ! | TATTGAGCTTGATGATTCAC+AGG | + | chr1.4:79165124-79165143 | MS.gene070992:intron | 35.0% |
| CCCACAACATCACAAAATCT+CGG | + | chr1.4:79164364-79164383 | MS.gene070992:CDS | 40.0% | |
| CTCAGTTAGATCTACACTGT+CGG | - | chr1.4:79165159-79165178 | None:intergenic | 40.0% | |
| GAAGCAAAGGAGACAAGAAA+TGG | + | chr1.4:79164553-79164572 | MS.gene070992:CDS | 40.0% | |
| TCACCTTGATGAAGTGAAGA+GGG | - | chr1.4:79164610-79164629 | None:intergenic | 40.0% | |
| TGCCCCACTTTCTAAATTTC+TGG | + | chr1.4:79164936-79164955 | MS.gene070992:CDS | 40.0% | |
| TTCACCTTGATGAAGTGAAG+AGG | - | chr1.4:79164611-79164630 | None:intergenic | 40.0% | |
| ! | AGATTTTGTGATGTTGTGGG+TGG | - | chr1.4:79164364-79164383 | None:intergenic | 40.0% |
| ! | CCGAGATTTTGTGATGTTGT+GGG | - | chr1.4:79164367-79164386 | None:intergenic | 40.0% |
| ! | TCCGAGATTTTGTGATGTTG+TGG | - | chr1.4:79164368-79164387 | None:intergenic | 40.0% |
| AAGAGGGAGAAGGGATCTAA+GGG | - | chr1.4:79164594-79164613 | None:intergenic | 45.0% | |
| ATACCTCACACTGCACACTA+TGG | - | chr1.4:79165029-79165048 | None:intergenic | 45.0% | |
| GATGAAGTGAAGAGGGAGAA+GGG | - | chr1.4:79164603-79164622 | None:intergenic | 45.0% | |
| TCTCCCTCTTCACTTCATCA+AGG | + | chr1.4:79164604-79164623 | MS.gene070992:CDS | 45.0% | |
| TGATGAAGTGAAGAGGGAGA+AGG | - | chr1.4:79164604-79164623 | None:intergenic | 45.0% | |
| ! | TTTTGTGATGTTGTGGGTGG+TGG | - | chr1.4:79164361-79164380 | None:intergenic | 45.0% |
| ACTCCATAGTGTGCAGTGTG+AGG | + | chr1.4:79165023-79165042 | MS.gene070992:CDS | 50.0% | |
| GAAGAGGGAGAAGGGATCTA+AGG | - | chr1.4:79164595-79164614 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 79164302 | 79165229 | 79164302 | ID=MS.gene070992 |
| chr1.4 | mRNA | 79164302 | 79165229 | 79164302 | ID=MS.gene070992.t1;Parent=MS.gene070992 |
| chr1.4 | exon | 79164302 | 79164625 | 79164302 | ID=MS.gene070992.t1.exon1;Parent=MS.gene070992.t1 |
| chr1.4 | CDS | 79164302 | 79164625 | 79164302 | ID=cds.MS.gene070992.t1;Parent=MS.gene070992.t1 |
| chr1.4 | exon | 79164715 | 79165044 | 79164715 | ID=MS.gene070992.t1.exon2;Parent=MS.gene070992.t1 |
| chr1.4 | CDS | 79164715 | 79165044 | 79164715 | ID=cds.MS.gene070992.t1;Parent=MS.gene070992.t1 |
| chr1.4 | exon | 79165146 | 79165229 | 79165146 | ID=MS.gene070992.t1.exon3;Parent=MS.gene070992.t1 |
| chr1.4 | CDS | 79165146 | 79165229 | 79165146 | ID=cds.MS.gene070992.t1;Parent=MS.gene070992.t1 |
| Gene Sequence |
| Protein sequence |
