AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene071035


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene071035.t1 MTR_7g070050 100.000 186 0 0 1 186 1 186 3.90e-138 383
MS.gene071035.t1 MTR_4g014460 68.156 179 51 3 1 178 36 209 2.62e-85 250
MS.gene071035.t1 MTR_3g071740 66.286 175 50 3 4 177 57 223 1.12e-78 234
MS.gene071035.t1 MTR_5g083270 66.667 177 49 4 4 178 37 205 6.01e-77 229
MS.gene071035.t1 MTR_8g027805 55.689 167 68 3 13 179 18 178 3.62e-64 196
MS.gene071035.t1 MTR_1g028380 56.805 169 67 3 11 179 17 179 7.20e-64 195
MS.gene071035.t1 MTR_3g090980 54.913 173 72 3 7 179 15 181 1.33e-62 192
MS.gene071035.t1 MTR_5g030500 58.788 165 65 2 13 176 44 206 1.27e-61 190
MS.gene071035.t1 MTR_1g016480 52.601 173 77 3 13 185 39 206 1.55e-60 187
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene071035.t1 AT2G38310 75.141 177 35 2 5 180 31 199 6.31e-86 252
MS.gene071035.t1 AT2G40330 66.471 170 53 2 9 177 44 210 1.85e-81 241
MS.gene071035.t1 AT5G05440 66.265 166 47 3 9 173 41 198 9.17e-76 226
MS.gene071035.t1 AT1G01360 56.471 170 68 2 16 185 24 187 3.50e-66 201
MS.gene071035.t1 AT5G53160 52.809 178 77 3 2 179 9 179 3.84e-64 196
MS.gene071035.t1 AT4G01026 56.707 164 65 3 17 180 27 184 2.76e-62 192
MS.gene071035.t1 AT2G26040 54.167 168 68 2 8 172 17 178 2.40e-60 186
MS.gene071035.t1 AT4G17870 51.667 180 81 2 1 179 5 179 4.72e-60 186
MS.gene071035.t1 AT4G27920 47.159 176 88 2 11 186 12 182 2.08e-57 179
MS.gene071035.t1 AT1G73000 50.595 168 77 3 11 172 34 201 2.71e-55 174
MS.gene071035.t1 AT5G45860 55.758 165 60 2 15 179 6 157 5.28e-51 162
MS.gene071035.t1 AT5G46790 53.757 173 77 2 7 178 38 208 4.17e-50 161
MS.gene071035.t1 AT5G46790 53.757 173 77 2 7 178 38 208 4.17e-50 161
MS.gene071035.t1 AT5G45870 56.209 153 60 1 26 178 10 155 3.41e-49 157
MS.gene071035.t1 AT4G18620 51.553 161 67 3 24 178 7 162 1.29e-39 132
MS.gene071035.t1 AT5G53160 53.571 112 50 2 2 113 9 118 3.21e-37 126

Find 68 sgRNAs with CRISPR-Local

Find 69 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGCGAAATTTGCTGAGAATT+TGG 0.111678 7.3:-34864130 MS.gene071035:CDS
TGGTAGCGGGCGATGGCGTC+GGG 0.286221 7.3:+34864615 None:intergenic
CGGCGGAGCAACACTGGTTT+GGG 0.304384 7.3:+34864577 None:intergenic
GGGACGGTGGTTGTTGGGTT+TGG 0.316747 7.3:+34864636 None:intergenic
GTGGTAGCGGGCGATGGCGT+CGG 0.350975 7.3:+34864614 None:intergenic
GCGTCGGGGACGGTGGTTGT+TGG 0.355449 7.3:+34864630 None:intergenic
GTATCTTCAGTGGTGTTTCC+AGG 0.355878 7.3:+34864198 None:intergenic
ACGGCGGAGCAACACTGGTT+TGG 0.359142 7.3:+34864576 None:intergenic
CGTCGGGGACGGTGGTTGTT+GGG 0.383221 7.3:+34864631 None:intergenic
TCTTCGTCGGAGATCGGACT+AGG 0.424642 7.3:+34864276 None:intergenic
GACTGCGTGGGTGTGGTAGC+GGG 0.430031 7.3:+34864602 None:intergenic
CAAATTTCGCGAGAGATTGA+AGG 0.431244 7.3:+34864142 None:intergenic
AGACTGCGTGGGTGTGGTAG+CGG 0.434046 7.3:+34864601 None:intergenic
GCTGACAGCGGCGGGGAGGC+CGG 0.435041 7.3:+34864404 None:intergenic
GCGGGCGATGGCGTCGGGGA+CGG 0.442801 7.3:+34864620 None:intergenic
CGTTCGGTGCTGACAGCGGC+GGG 0.446228 7.3:+34864396 None:intergenic
CGTGAAGTCCGCGTCATCTC+CGG 0.470592 7.3:-34864423 MS.gene071035:CDS
TTGGGGAGACTGCGTGGGTG+TGG 0.471898 7.3:+34864595 None:intergenic
GTGGGTGTGGTAGCGGGCGA+TGG 0.478652 7.3:+34864608 None:intergenic
GTCTAAGACTTCGAGACGTT+CGG 0.490124 7.3:+34864380 None:intergenic
CGGAGATGACGCGGACTTCA+CGG 0.493833 7.3:+34864424 None:intergenic
GGCGATGGCGTCGGGGACGG+TGG 0.507167 7.3:+34864623 None:intergenic
GTCATCAGCTTCAGCATGAT+CGG 0.509660 7.3:-34864345 MS.gene071035:CDS
ACGGACCAGACGGTGGAGAC+TGG 0.510545 7.3:+34864534 None:intergenic
CCGCACCAGTCTCCACCGTC+TGG 0.515481 7.3:-34864539 MS.gene071035:CDS
TGTTTGTTGATACTATTCTT+CGG 0.519630 7.3:-34864170 MS.gene071035:CDS
AGTCCGATCTCCGACGAAGA+TGG 0.524898 7.3:-34864273 MS.gene071035:CDS
ACGAAGTGTTTGTAGGCTTG+TGG 0.526533 7.3:+34864492 None:intergenic
TGCCGTCGCCGAGGATTACA+TGG 0.531287 7.3:+34864460 None:intergenic
TTGCCATCTTCGTCGGAGAT+CGG 0.532468 7.3:+34864270 None:intergenic
CGGGGAGGCCGGAGATGACG+CGG 0.540759 7.3:+34864415 None:intergenic
GAAGATGGCAACCACCGCTC+CGG 0.541680 7.3:-34864258 MS.gene071035:CDS
GGATGACGGCGGAGCAACAC+TGG 0.549088 7.3:+34864571 None:intergenic
AGACTGGTGCGGCGATATGT+TGG 0.553693 7.3:+34864550 None:intergenic
ATTGTCGAAGCGACGGACTA+CGG 0.565639 7.3:+34864515 None:intergenic
TATGTTGTTGATGTTCCACC+TGG 0.568525 7.3:-34864216 MS.gene071035:CDS
CAACCACCGTGCCGGAGCGG+TGG 0.569837 7.3:+34864247 None:intergenic
ACGTTCGGTGCTGACAGCGG+CGG 0.570809 7.3:+34864395 None:intergenic
CGGTGCTGACAGCGGCGGGG+AGG 0.581007 7.3:+34864400 None:intergenic
GCGGTGGTTGCCATCTTCGT+CGG 0.582833 7.3:+34864263 None:intergenic
GGACTCAACAACCACCGTGC+CGG 0.583858 7.3:+34864239 None:intergenic
TGCCATGTAATCCTCGGCGA+CGG 0.587077 7.3:-34864462 MS.gene071035:CDS
TGCCGACGTTGCCGTCGCCG+AGG 0.590423 7.3:+34864451 None:intergenic
ACTGGTTTGGGGAGACTGCG+TGG 0.592349 7.3:+34864589 None:intergenic
CTTCGTCGGAGATCGGACTA+GGG 0.595738 7.3:+34864277 None:intergenic
AAAAGCTGCCATGTAATCCT+CGG 0.597517 7.3:-34864468 MS.gene071035:CDS
TCTTCAGTGGTGTTTCCAGG+TGG 0.600688 7.3:+34864201 None:intergenic
AGGTGGAACATCAACAACAT+AGG 0.606670 7.3:+34864218 None:intergenic
TGGCAACCACCGCTCCGGCA+CGG 0.610622 7.3:-34864253 MS.gene071035:CDS
ATCCTCGGCGACGGCAACGT+CGG 0.617289 7.3:-34864453 MS.gene071035:CDS
CTGGTTTGGGGAGACTGCGT+GGG 0.619515 7.3:+34864590 None:intergenic
GGTGAAGAGTGGTGACAGAA+CGG 0.622514 7.3:+34864298 None:intergenic
GCGACGGACTACGGACCAGA+CGG 0.625454 7.3:+34864524 None:intergenic
AAACACACATGTATCTTCAG+TGG 0.628787 7.3:+34864188 None:intergenic
CAACCACCGCTCCGGCACGG+TGG 0.633877 7.3:-34864250 MS.gene071035:CDS
CAACAACCACCGTGCCGGAG+CGG 0.635412 7.3:+34864244 None:intergenic
GGTAGCGGGCGATGGCGTCG+GGG 0.638452 7.3:+34864616 None:intergenic
ACGGACTACGGACCAGACGG+TGG 0.644893 7.3:+34864527 None:intergenic
GAGACGTTCGGTGCTGACAG+CGG 0.650293 7.3:+34864392 None:intergenic
GTTCGGTGCTGACAGCGGCG+GGG 0.655778 7.3:+34864397 None:intergenic
GATCGGACTAGGGTGAAGAG+TGG 0.660402 7.3:+34864287 None:intergenic
CTTGTGGATTGTCGAAGCGA+CGG 0.660521 7.3:+34864508 None:intergenic
TGCGGCGATATGTTGGATGA+CGG 0.663571 7.3:+34864557 None:intergenic
ATCAGCTTCAGCATGATCGG+AGG 0.664656 7.3:-34864342 MS.gene071035:CDS
GGCGGAGCAACACTGGTTTG+GGG 0.687344 7.3:+34864578 None:intergenic
GGCGATATGTTGGATGACGG+CGG 0.710240 7.3:+34864560 None:intergenic
CCAGACGGTGGAGACTGGTG+CGG 0.715437 7.3:+34864539 None:intergenic
CAGAACGGTAGTTAGCAAGA+CGG 0.719529 7.3:+34864313 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TGTTTGTTGATACTATTCTT+CGG - chr7.3:34864576-34864595 MS.gene071035:CDS 25.0%
AAACACACATGTATCTTCAG+TGG + chr7.3:34864561-34864580 None:intergenic 35.0%
AAAAGCTGCCATGTAATCCT+CGG - chr7.3:34864278-34864297 MS.gene071035:CDS 40.0%
AGGTGGAACATCAACAACAT+AGG + chr7.3:34864531-34864550 None:intergenic 40.0%
CAAATTTCGCGAGAGATTGA+AGG + chr7.3:34864607-34864626 None:intergenic 40.0%
TATGTTGTTGATGTTCCACC+TGG - chr7.3:34864530-34864549 MS.gene071035:CDS 40.0%
!! CGCGAAATTTGCTGAGAATT+TGG - chr7.3:34864616-34864635 MS.gene071035:CDS 40.0%
!! GCTTTTGACGAAGTGTTTGT+AGG + chr7.3:34864264-34864283 None:intergenic 40.0%
CAGAACGGTAGTTAGCAAGA+CGG + chr7.3:34864436-34864455 None:intergenic 45.0%
GTATCTTCAGTGGTGTTTCC+AGG + chr7.3:34864551-34864570 None:intergenic 45.0%
GTCATCAGCTTCAGCATGAT+CGG - chr7.3:34864401-34864420 MS.gene071035:CDS 45.0%
! ACGAAGTGTTTGTAGGCTTG+TGG + chr7.3:34864257-34864276 None:intergenic 45.0%
! GTCTAAGACTTCGAGACGTT+CGG + chr7.3:34864369-34864388 None:intergenic 45.0%
ATCAGCTTCAGCATGATCGG+AGG - chr7.3:34864404-34864423 MS.gene071035:CDS 50.0%
GGTGAAGAGTGGTGACAGAA+CGG + chr7.3:34864451-34864470 None:intergenic 50.0%
TCTTCAGTGGTGTTTCCAGG+TGG + chr7.3:34864548-34864567 None:intergenic 50.0%
TGCGGCGATATGTTGGATGA+CGG + chr7.3:34864192-34864211 None:intergenic 50.0%
TTGCCATCTTCGTCGGAGAT+CGG + chr7.3:34864479-34864498 None:intergenic 50.0%
! CTTGTGGATTGTCGAAGCGA+CGG + chr7.3:34864241-34864260 None:intergenic 50.0%
!! ATTGTCGAAGCGACGGACTA+CGG + chr7.3:34864234-34864253 None:intergenic 50.0%
AGACTGGTGCGGCGATATGT+TGG + chr7.3:34864199-34864218 None:intergenic 55.0%
AGTCCGATCTCCGACGAAGA+TGG - chr7.3:34864473-34864492 MS.gene071035:CDS 55.0%
CTTCGTCGGAGATCGGACTA+GGG + chr7.3:34864472-34864491 None:intergenic 55.0%
TCTTCGTCGGAGATCGGACT+AGG + chr7.3:34864473-34864492 None:intergenic 55.0%
TGCCATGTAATCCTCGGCGA+CGG - chr7.3:34864284-34864303 MS.gene071035:CDS 55.0%
! GATCGGACTAGGGTGAAGAG+TGG + chr7.3:34864462-34864481 None:intergenic 55.0%
! GGCGATATGTTGGATGACGG+CGG + chr7.3:34864189-34864208 None:intergenic 55.0%
AGACTGCGTGGGTGTGGTAG+CGG + chr7.3:34864148-34864167 None:intergenic 60.0%
CGGAGATGACGCGGACTTCA+CGG + chr7.3:34864325-34864344 None:intergenic 60.0%
CGTGAAGTCCGCGTCATCTC+CGG - chr7.3:34864323-34864342 MS.gene071035:CDS 60.0%
GAAGATGGCAACCACCGCTC+CGG - chr7.3:34864488-34864507 MS.gene071035:CDS 60.0%
GCGGTGGTTGCCATCTTCGT+CGG + chr7.3:34864486-34864505 None:intergenic 60.0%
GGACTCAACAACCACCGTGC+CGG + chr7.3:34864510-34864529 None:intergenic 60.0%
GGGACGGTGGTTGTTGGGTT+TGG + chr7.3:34864113-34864132 None:intergenic 60.0%
TGCCGTCGCCGAGGATTACA+TGG + chr7.3:34864289-34864308 None:intergenic 60.0%
! ACGGCGGAGCAACACTGGTT+TGG + chr7.3:34864173-34864192 None:intergenic 60.0%
!! ACTGGTTTGGGGAGACTGCG+TGG + chr7.3:34864160-34864179 None:intergenic 60.0%
!! CGGCGGAGCAACACTGGTTT+GGG + chr7.3:34864172-34864191 None:intergenic 60.0%
!! CTGGTTTGGGGAGACTGCGT+GGG + chr7.3:34864159-34864178 None:intergenic 60.0%
!! GAGACGTTCGGTGCTGACAG+CGG + chr7.3:34864357-34864376 None:intergenic 60.0%
!! GGCGGAGCAACACTGGTTTG+GGG + chr7.3:34864171-34864190 None:intergenic 60.0%
ACGGACCAGACGGTGGAGAC+TGG + chr7.3:34864215-34864234 None:intergenic 65.0%
ATCCTCGGCGACGGCAACGT+CGG - chr7.3:34864293-34864312 MS.gene071035:CDS 65.0%
CAACAACCACCGTGCCGGAG+CGG + chr7.3:34864505-34864524 None:intergenic 65.0%
CCAGACGGTGGAGACTGGTG+CGG + chr7.3:34864210-34864229 None:intergenic 65.0%
CGTCGGGGACGGTGGTTGTT+GGG + chr7.3:34864118-34864137 None:intergenic 65.0%
GACTGCGTGGGTGTGGTAGC+GGG + chr7.3:34864147-34864166 None:intergenic 65.0%
TTGGGGAGACTGCGTGGGTG+TGG + chr7.3:34864154-34864173 None:intergenic 65.0%
! ACGGACTACGGACCAGACGG+TGG + chr7.3:34864222-34864241 None:intergenic 65.0%
! GGATGACGGCGGAGCAACAC+TGG + chr7.3:34864178-34864197 None:intergenic 65.0%
!! ACGTTCGGTGCTGACAGCGG+CGG + chr7.3:34864354-34864373 None:intergenic 65.0%
!! GCGACGGACTACGGACCAGA+CGG + chr7.3:34864225-34864244 None:intergenic 65.0%
CCGCACCAGTCTCCACCGTC+TGG - chr7.3:34864207-34864226 MS.gene071035:CDS 70.0%
GCGTCGGGGACGGTGGTTGT+TGG + chr7.3:34864119-34864138 None:intergenic 70.0%
TGGCAACCACCGCTCCGGCA+CGG - chr7.3:34864493-34864512 MS.gene071035:CDS 70.0%
!! CGTTCGGTGCTGACAGCGGC+GGG + chr7.3:34864353-34864372 None:intergenic 70.0%
!! GTGGGTGTGGTAGCGGGCGA+TGG + chr7.3:34864141-34864160 None:intergenic 70.0%
!! GTGGTAGCGGGCGATGGCGT+CGG + chr7.3:34864135-34864154 None:intergenic 70.0%
!! GTTCGGTGCTGACAGCGGCG+GGG + chr7.3:34864352-34864371 None:intergenic 70.0%
!! TGGTAGCGGGCGATGGCGTC+GGG + chr7.3:34864134-34864153 None:intergenic 70.0%
CAACCACCGCTCCGGCACGG+TGG - chr7.3:34864496-34864515 MS.gene071035:CDS 75.0%
CAACCACCGTGCCGGAGCGG+TGG + chr7.3:34864502-34864521 None:intergenic 75.0%
CGGGGAGGCCGGAGATGACG+CGG + chr7.3:34864334-34864353 None:intergenic 75.0%
TGCCGACGTTGCCGTCGCCG+AGG + chr7.3:34864298-34864317 None:intergenic 75.0%
!! GGTAGCGGGCGATGGCGTCG+GGG + chr7.3:34864133-34864152 None:intergenic 75.0%
! GCTGACAGCGGCGGGGAGGC+CGG + chr7.3:34864345-34864364 None:intergenic 80.0%
!! GCGGGCGATGGCGTCGGGGA+CGG + chr7.3:34864129-34864148 None:intergenic 80.0%
!! GGCGATGGCGTCGGGGACGG+TGG + chr7.3:34864126-34864145 None:intergenic 80.0%
!!! CGGTGCTGACAGCGGCGGGG+AGG + chr7.3:34864349-34864368 None:intergenic 80.0%


Chromosome Type Strat End Strand Name
chr7.3 gene 34864104 34864664 34864104 ID=MS.gene071035
chr7.3 mRNA 34864104 34864664 34864104 ID=MS.gene071035.t1;Parent=MS.gene071035
chr7.3 exon 34864104 34864664 34864104 ID=MS.gene071035.t1.exon1;Parent=MS.gene071035.t1
chr7.3 CDS 34864104 34864664 34864104 ID=cds.MS.gene071035.t1;Parent=MS.gene071035.t1
Gene Sequence

>MS.gene071035

ATGTTACCAAACCCAACAACCACCGTCCCCGACGCCATCGCCCGCTACCACACCCACGCAGTCTCCCCAAACCAGTGTTGCTCCGCCGTCATCCAACATATCGCCGCACCAGTCTCCACCGTCTGGTCCGTAGTCCGTCGCTTCGACAATCCACAAGCCTACAAACACTTCGTCAAAAGCTGCCATGTAATCCTCGGCGACGGCAACGTCGGCACTCTCCGTGAAGTCCGCGTCATCTCCGGCCTCCCCGCCGCTGTCAGCACCGAACGTCTCGAAGTCTTAGACGATGAACGTCACGTCATCAGCTTCAGCATGATCGGAGGTGATCACCGTCTTGCTAACTACCGTTCTGTCACCACTCTTCACCCTAGTCCGATCTCCGACGAAGATGGCAACCACCGCTCCGGCACGGTGGTTGTTGAGTCCTATGTTGTTGATGTTCCACCTGGAAACACCACTGAAGATACATGTGTGTTTGTTGATACTATTCTTCGGTGCAACCTTCAATCTCTCGCGAAATTTGCTGAGAATTTGGCTTCAACGAGATCAAATCAACGATAA

Protein sequence

>MS.gene071035.t1

MLPNPTTTVPDAIARYHTHAVSPNQCCSAVIQHIAAPVSTVWSVVRRFDNPQAYKHFVKSCHVILGDGNVGTLREVRVISGLPAAVSTERLEVLDDERHVISFSMIGGDHRLANYRSVTTLHPSPISDEDGNHRSGTVVVESYVVDVPPGNTTEDTCVFVDTILRCNLQSLAKFAENLASTRSNQR