AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene054810


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene054810.t1 MTR_5g030500 98.624 218 1 1 1 218 1 216 2.37e-160 442
MS.gene054810.t1 MTR_1g016480 55.429 175 72 3 33 206 26 195 5.66e-66 202
MS.gene054810.t1 MTR_3g071740 52.663 169 74 2 49 217 69 231 4.18e-62 193
MS.gene054810.t1 MTR_5g083270 49.505 202 87 5 16 208 8 203 3.34e-61 190
MS.gene054810.t1 MTR_4g014460 54.070 172 74 3 41 211 43 210 3.14e-56 178
MS.gene054810.t1 MTR_7g070050 58.788 165 65 2 46 208 13 176 3.82e-53 169
MS.gene054810.t1 MTR_8g027805 45.856 181 90 4 30 208 1 175 3.30e-49 159
MS.gene054810.t1 MTR_3g090980 48.795 166 78 3 45 209 20 179 3.45e-49 159
MS.gene054810.t1 MTR_1g028380 49.398 166 77 3 45 209 18 177 7.30e-49 158
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene054810.t1 AT5G46790 66.507 209 68 1 1 209 1 207 1.04e-91 268
MS.gene054810.t1 AT5G46790 66.507 209 68 1 1 209 1 207 1.04e-91 268
MS.gene054810.t1 AT4G17870 69.149 188 54 2 30 216 1 185 2.14e-91 266
MS.gene054810.t1 AT2G26040 57.558 172 71 2 33 204 9 178 1.25e-67 206
MS.gene054810.t1 AT1G73000 52.432 185 82 3 31 209 22 206 2.37e-66 204
MS.gene054810.t1 AT5G05440 54.938 162 67 2 46 207 45 200 4.36e-61 190
MS.gene054810.t1 AT2G40330 51.534 163 78 1 50 212 52 213 4.04e-57 180
MS.gene054810.t1 AT2G38310 55.952 168 68 1 50 217 43 204 4.37e-52 167
MS.gene054810.t1 AT4G01026 51.250 160 71 3 50 208 27 180 3.50e-50 162
MS.gene054810.t1 AT5G53160 48.780 164 79 2 45 208 18 176 1.32e-49 160
MS.gene054810.t1 AT1G01360 47.305 167 81 3 43 208 18 178 2.90e-49 159
MS.gene054810.t1 AT4G27920 47.879 165 80 3 45 208 13 172 9.34e-49 158
MS.gene054810.t1 AT5G45860 54.000 150 64 2 59 208 10 154 6.25e-39 132
MS.gene054810.t1 AT5G45870 52.667 150 65 2 59 208 10 153 1.28e-38 131
MS.gene054810.t1 AT4G18620 48.408 157 73 3 57 208 7 160 1.44e-33 118
MS.gene054810.t1 AT5G53160 50.485 103 49 1 45 147 18 118 1.21e-29 108

Find 72 sgRNAs with CRISPR-Local

Find 75 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTACTTCCGTTCACGGTTT+CGG 0.321183 5.2:-22339 MS.gene054810:CDS
TGAGATCTGGACCGTTGTTT+TGG 0.357741 5.2:-22298 MS.gene054810:CDS
GACGACGAACGTCGTGTTAC+CGG 0.369543 5.2:-22408 MS.gene054810:CDS
CGTTCCGTTACTTCCGTTCA+CGG 0.394929 5.2:-22345 MS.gene054810:CDS
GGAGGCCGGAGGGGAGTGTT+AGG 0.404211 5.2:+22712 None:intergenic
TCCTCTTCAGTATTACCTTC+AGG 0.411978 5.2:+22255 None:intergenic
AGAAGCCTGGAGCATTGGTT+GGG 0.412638 5.2:+22627 None:intergenic
GTGATAACGGTGGTGAGATC+TGG 0.416625 5.2:-22311 MS.gene054810:CDS
CTCTCGGTGCTAGTCGCCGC+CGG 0.416895 5.2:+22444 None:intergenic
ACCGTCACGGTTCTTTCCTT+CGG 0.424743 5.2:+22173 None:intergenic
TCACACCACACCTACCTCAT+AGG 0.430563 5.2:-22651 MS.gene054810:CDS
GGTGGAAGCGATGGCGGTGG+AGG 0.430580 5.2:+22776 None:intergenic
ATGAAGTGTTTGTAGATTTG+CGG 0.443636 5.2:+22543 None:intergenic
GTCGGAATCTTGATCGGAGG+TGG 0.456152 5.2:+22758 None:intergenic
ACGCGCCGCCGCAAACCGTC+TGG 0.474731 5.2:-22590 MS.gene054810:CDS
CAATGTCATCTCCGGTCTCC+CGG 0.474823 5.2:-22463 MS.gene054810:CDS
GCTAGTCGCCGCCGGGAGAC+CGG 0.484689 5.2:+22452 None:intergenic
TCCGTTCACGGTTTCGGTGA+CGG 0.493319 5.2:-22333 MS.gene054810:CDS
ACGACGGACCAGACGGTTTG+CGG 0.500991 5.2:+22582 None:intergenic
AGACGGTTTGCGGCGGCGCG+TGG 0.502746 5.2:+22592 None:intergenic
AAAAGCTGCTCTCTCAAAGA+AGG 0.506128 5.2:-22519 MS.gene054810:CDS
GGAGGTGGAAGCGATGGCGG+TGG 0.507691 5.2:+22773 None:intergenic
GAGAAGCCTGGAGCATTGGT+TGG 0.510173 5.2:+22626 None:intergenic
GATGCGTTGAGCGAGAAGCC+TGG 0.510312 5.2:+22614 None:intergenic
GAAGCCTGGAGCATTGGTTG+GGG 0.513167 5.2:+22628 None:intergenic
GAGCGAGAAGCCTGGAGCAT+TGG 0.514906 5.2:+22622 None:intergenic
AGCATTGGTTGGGGCCTATG+AGG 0.517600 5.2:+22637 None:intergenic
CGCGACGTCAATGTCATCTC+CGG 0.522159 5.2:-22471 MS.gene054810:CDS
GTCAAGAACGTCGAGTCTCT+CGG 0.524491 5.2:+22428 None:intergenic
ACCGCCGATGATACTGAAAC+CGG 0.530952 5.2:+22389 None:intergenic
GGGAGTCGAATTCGTGTTGA+CGG 0.541010 5.2:+22691 None:intergenic
GGTTTCGGTGACGGTGATAA+CGG 0.543393 5.2:-22324 MS.gene054810:CDS
TAGGCCCCAACCAATGCTCC+AGG 0.544690 5.2:-22632 MS.gene054810:CDS
CGGAACGGTAATTCTTCAAA+CGG 0.546020 5.2:+22361 None:intergenic
GAATTCGTGTTGACGGAGGC+CGG 0.548211 5.2:+22698 None:intergenic
TTGGTTGGGGCCTATGAGGT+AGG 0.550389 5.2:+22641 None:intergenic
TTTGCGGTTTGTCGAAGCTA+CGG 0.554765 5.2:+22559 None:intergenic
ATCGGAGGTGGAAGCGATGG+CGG 0.560121 5.2:+22770 None:intergenic
TCTCGGTGCTAGTCGCCGCC+GGG 0.563257 5.2:+22445 None:intergenic
TGTGGTGTGAGTTGATGAAA+GGG 0.564889 5.2:+22664 None:intergenic
CGTGTTGACGGAGGCCGGAG+GGG 0.570765 5.2:+22703 None:intergenic
TTGATCGGAGGTGGAAGCGA+TGG 0.571051 5.2:+22767 None:intergenic
TTCGTGTTGACGGAGGCCGG+AGG 0.572606 5.2:+22701 None:intergenic
ACCGTCACCGAAACCGTGAA+CGG 0.574955 5.2:+22332 None:intergenic
TCGTGTTGACGGAGGCCGGA+GGG 0.581954 5.2:+22702 None:intergenic
TGAGTTGATGAAAGGGATGA+GGG 0.584437 5.2:+22671 None:intergenic
GAAACCGTGAACGGAAGTAA+CGG 0.584827 5.2:+22341 None:intergenic
GGAAAGAACCGTGACGGTGA+CGG 0.588055 5.2:-22168 MS.gene054810:CDS
CTGACTTACCGTCACCGTCA+CGG 0.588675 5.2:+22160 None:intergenic
CACCACCTAACACTCCCCTC+CGG 0.591738 5.2:-22717 MS.gene054810:CDS
GTGTTAGGTGGTGTTGTGTG+CGG 0.591821 5.2:+22727 None:intergenic
TACGTCGTTGATGTTCCTGA+AGG 0.592427 5.2:-22270 MS.gene054810:CDS
GTGAGTTGATGAAAGGGATG+AGG 0.592854 5.2:+22670 None:intergenic
TTCGTCGGAATCTTGATCGG+AGG 0.592857 5.2:+22755 None:intergenic
GTTACCGGTTTCAGTATCAT+CGG 0.595373 5.2:-22393 MS.gene054810:CDS
GTGTGGTGTGAGTTGATGAA+AGG 0.597891 5.2:+22663 None:intergenic
TTAGGTGGTGTTGTGTGCGG+TGG 0.600549 5.2:+22730 None:intergenic
GGCCGGAGGGGAGTGTTAGG+TGG 0.606358 5.2:+22715 None:intergenic
AAGCTTGCGTCTGTCACCGA+AGG 0.615885 5.2:-22189 MS.gene054810:CDS
TCCTGAAGGTAATACTGAAG+AGG 0.617871 5.2:-22256 MS.gene054810:CDS
TGGGGCCTATGAGGTAGGTG+TGG 0.623679 5.2:+22646 None:intergenic
GCTACGGACGACGGACCAGA+CGG 0.637251 5.2:+22575 None:intergenic
ACGGACCAGACGGTTTGCGG+CGG 0.644883 5.2:+22585 None:intergenic
ACCGGTTTCAGTATCATCGG+CGG 0.669189 5.2:-22390 MS.gene054810:CDS
CTGAAGATTGAGCTTCACAA+CGG 0.671146 5.2:+22212 None:intergenic
ACCGAAGGAAAGAACCGTGA+CGG 0.674541 5.2:-22174 MS.gene054810:CDS
AGTCGAATTCGTGTTGACGG+AGG 0.683583 5.2:+22694 None:intergenic
TTTGTCGAAGCTACGGACGA+CGG 0.690180 5.2:+22566 None:intergenic
GACGTAAGATTCCAAAACAA+CGG 0.691402 5.2:+22287 None:intergenic
TTCGGTGACGGTGATAACGG+TGG 0.691549 5.2:-22321 MS.gene054810:CDS
TGTCATCTCCGGTCTCCCGG+CGG 0.695228 5.2:-22460 MS.gene054810:CDS
CGTGAACGGAAGTAACGGAA+CGG 0.712612 5.2:+22346 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! ATGAAGTGTTTGTAGATTTG+CGG + chr5.2:22407-22426 None:intergenic 30.0%
! GAAGGTTTTCAAATGAAAGT+CGG - chr5.2:22446-22465 MS.gene054810:CDS 30.0%
GACGTAAGATTCCAAAACAA+CGG + chr5.2:22663-22682 None:intergenic 35.0%
AAAAGCTGCTCTCTCAAAGA+AGG - chr5.2:22428-22447 MS.gene054810:CDS 40.0%
CGGAACGGTAATTCTTCAAA+CGG + chr5.2:22589-22608 None:intergenic 40.0%
CTGAAGATTGAGCTTCACAA+CGG + chr5.2:22738-22757 None:intergenic 40.0%
TCCTCTTCAGTATTACCTTC+AGG + chr5.2:22695-22714 None:intergenic 40.0%
TCCTGAAGGTAATACTGAAG+AGG - chr5.2:22691-22710 MS.gene054810:CDS 40.0%
! GTTTTCGTCGGAATCTTGAT+CGG + chr5.2:22198-22217 None:intergenic 40.0%
!! GTTACCGGTTTCAGTATCAT+CGG - chr5.2:22554-22573 MS.gene054810:CDS 40.0%
!! TGAGTTGATGAAAGGGATGA+GGG + chr5.2:22279-22298 None:intergenic 40.0%
!! TGTGGTGTGAGTTGATGAAA+GGG + chr5.2:22286-22305 None:intergenic 40.0%
GAAACCGTGAACGGAAGTAA+CGG + chr5.2:22609-22628 None:intergenic 45.0%
GTTACTTCCGTTCACGGTTT+CGG - chr5.2:22608-22627 MS.gene054810:CDS 45.0%
TACGTCGTTGATGTTCCTGA+AGG - chr5.2:22677-22696 MS.gene054810:CDS 45.0%
TTTGCGGTTTGTCGAAGCTA+CGG + chr5.2:22391-22410 None:intergenic 45.0%
! TGAGATCTGGACCGTTGTTT+TGG - chr5.2:22649-22668 MS.gene054810:CDS 45.0%
!! GTGAGTTGATGAAAGGGATG+AGG + chr5.2:22280-22299 None:intergenic 45.0%
!! GTGTGGTGTGAGTTGATGAA+AGG + chr5.2:22287-22306 None:intergenic 45.0%
ACCGAAGGAAAGAACCGTGA+CGG - chr5.2:22773-22792 MS.gene054810:CDS 50.0%
ACCGCCGATGATACTGAAAC+CGG + chr5.2:22561-22580 None:intergenic 50.0%
ACCGTCACGGTTCTTTCCTT+CGG + chr5.2:22777-22796 None:intergenic 50.0%
CGTTCCGTTACTTCCGTTCA+CGG - chr5.2:22602-22621 MS.gene054810:CDS 50.0%
GGGAGTCGAATTCGTGTTGA+CGG + chr5.2:22259-22278 None:intergenic 50.0%
GTCAAGAACGTCGAGTCTCT+CGG + chr5.2:22522-22541 None:intergenic 50.0%
TCACACCACACCTACCTCAT+AGG - chr5.2:22296-22315 MS.gene054810:CDS 50.0%
TTCGTCGGAATCTTGATCGG+AGG + chr5.2:22195-22214 None:intergenic 50.0%
TTTGTCGAAGCTACGGACGA+CGG + chr5.2:22384-22403 None:intergenic 50.0%
! ACCGGTTTCAGTATCATCGG+CGG - chr5.2:22557-22576 MS.gene054810:CDS 50.0%
! AGTCGAATTCGTGTTGACGG+AGG + chr5.2:22256-22275 None:intergenic 50.0%
! CGTGAACGGAAGTAACGGAA+CGG + chr5.2:22604-22623 None:intergenic 50.0%
! GGTTTCGGTGACGGTGATAA+CGG - chr5.2:22623-22642 MS.gene054810:CDS 50.0%
! TTGTGTGCGGTGGTTTTCGT+CGG + chr5.2:22210-22229 None:intergenic 50.0%
!! AGAAGCCTGGAGCATTGGTT+GGG + chr5.2:22323-22342 None:intergenic 50.0%
!! GTGATAACGGTGGTGAGATC+TGG - chr5.2:22636-22655 MS.gene054810:CDS 50.0%
!! GTGTTAGGTGGTGTTGTGTG+CGG + chr5.2:22223-22242 None:intergenic 50.0%
AAGCTTGCGTCTGTCACCGA+AGG - chr5.2:22758-22777 MS.gene054810:CDS 55.0%
ACCGTCACCGAAACCGTGAA+CGG + chr5.2:22618-22637 None:intergenic 55.0%
CAATGTCATCTCCGGTCTCC+CGG - chr5.2:22484-22503 MS.gene054810:CDS 55.0%
CGCGACGTCAATGTCATCTC+CGG - chr5.2:22476-22495 MS.gene054810:CDS 55.0%
CTGACTTACCGTCACCGTCA+CGG + chr5.2:22790-22809 None:intergenic 55.0%
GACGACGAACGTCGTGTTAC+CGG - chr5.2:22539-22558 MS.gene054810:CDS 55.0%
GGAAAGAACCGTGACGGTGA+CGG - chr5.2:22779-22798 MS.gene054810:CDS 55.0%
GTCGGAATCTTGATCGGAGG+TGG + chr5.2:22192-22211 None:intergenic 55.0%
TCCGTTCACGGTTTCGGTGA+CGG - chr5.2:22614-22633 MS.gene054810:CDS 55.0%
TTGATCGGAGGTGGAAGCGA+TGG + chr5.2:22183-22202 None:intergenic 55.0%
TTGGTTGGGGCCTATGAGGT+AGG + chr5.2:22309-22328 None:intergenic 55.0%
! GAATTCGTGTTGACGGAGGC+CGG + chr5.2:22252-22271 None:intergenic 55.0%
!! AGCATTGGTTGGGGCCTATG+AGG + chr5.2:22313-22332 None:intergenic 55.0%
!! GAAGCCTGGAGCATTGGTTG+GGG + chr5.2:22322-22341 None:intergenic 55.0%
!! GAGAAGCCTGGAGCATTGGT+TGG + chr5.2:22324-22343 None:intergenic 55.0%
!! TTAGGTGGTGTTGTGTGCGG+TGG + chr5.2:22220-22239 None:intergenic 55.0%
!! TTCGGTGACGGTGATAACGG+TGG - chr5.2:22626-22645 MS.gene054810:CDS 55.0%
ACGACGGACCAGACGGTTTG+CGG + chr5.2:22368-22387 None:intergenic 60.0%
ATCGGAGGTGGAAGCGATGG+CGG + chr5.2:22180-22199 None:intergenic 60.0%
CACCACCTAACACTCCCCTC+CGG - chr5.2:22230-22249 MS.gene054810:CDS 60.0%
GAGCGAGAAGCCTGGAGCAT+TGG + chr5.2:22328-22347 None:intergenic 60.0%
GATGCGTTGAGCGAGAAGCC+TGG + chr5.2:22336-22355 None:intergenic 60.0%
TAGGCCCCAACCAATGCTCC+AGG - chr5.2:22315-22334 MS.gene054810:CDS 60.0%
TGGGGCCTATGAGGTAGGTG+TGG + chr5.2:22304-22323 None:intergenic 60.0%
ACGGACCAGACGGTTTGCGG+CGG + chr5.2:22365-22384 None:intergenic 65.0%
GCTACGGACGACGGACCAGA+CGG + chr5.2:22375-22394 None:intergenic 65.0%
TGTCATCTCCGGTCTCCCGG+CGG - chr5.2:22487-22506 MS.gene054810:CDS 65.0%
! TCGTGTTGACGGAGGCCGGA+GGG + chr5.2:22248-22267 None:intergenic 65.0%
! TTCGTGTTGACGGAGGCCGG+AGG + chr5.2:22249-22268 None:intergenic 65.0%
! CGTGTTGACGGAGGCCGGAG+GGG + chr5.2:22247-22266 None:intergenic 70.0%
! GGAGGTGGAAGCGATGGCGG+TGG + chr5.2:22177-22196 None:intergenic 70.0%
! GGTGGAAGCGATGGCGGTGG+AGG + chr5.2:22174-22193 None:intergenic 70.0%
!! CTCTCGGTGCTAGTCGCCGC+CGG + chr5.2:22506-22525 None:intergenic 70.0%
!! GGAGGCCGGAGGGGAGTGTT+AGG + chr5.2:22238-22257 None:intergenic 70.0%
!! GGCCGGAGGGGAGTGTTAGG+TGG + chr5.2:22235-22254 None:intergenic 70.0%
!! TCTCGGTGCTAGTCGCCGCC+GGG + chr5.2:22505-22524 None:intergenic 70.0%
ACGCGCCGCCGCAAACCGTC+TGG - chr5.2:22357-22376 MS.gene054810:CDS 75.0%
AGACGGTTTGCGGCGGCGCG+TGG + chr5.2:22358-22377 None:intergenic 75.0%
GCTAGTCGCCGCCGGGAGAC+CGG + chr5.2:22498-22517 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 22155 22814 22155 ID=MS.gene054810
chr5.2 mRNA 22155 22814 22155 ID=MS.gene054810.t1;Parent=MS.gene054810
chr5.2 exon 22155 22814 22155 ID=MS.gene054810.t1.exon1;Parent=MS.gene054810.t1
chr5.2 CDS 22155 22814 22155 ID=cds.MS.gene054810.t1;Parent=MS.gene054810.t1
Gene Sequence

>MS.gene054810

ATGGAGAAAGCAGAGTCCTCCACCGCCATCGCTTCCACCTCCGATCAAGATTCCGACGAAAACCACCGCACACAACACCACCTAACACTCCCCTCCGGCCTCCGTCAACACGAATTCGACTCCCTCATCCCTTTCATCAACTCACACCACACCTACCTCATAGGCCCCAACCAATGCTCCAGGCTTCTCGCTCAACGCATCCACGCGCCGCCGCAAACCGTCTGGTCCGTCGTCCGTAGCTTCGACAAACCGCAAATCTACAAACACTTCATCAAAAGCTGCTCTCTCAAAGAAGGTTTTCAAATGAAAGTCGGTTGCACTCGCGACGTCAATGTCATCTCCGGTCTCCCGGCGGCGACTAGCACCGAGAGACTCGACGTTCTTGACGACGAACGTCGTGTTACCGGTTTCAGTATCATCGGCGGTGAACACCGTTTGAAGAATTACCGTTCCGTTACTTCCGTTCACGGTTTCGGTGACGGTGATAACGGTGGTGAGATCTGGACCGTTGTTTTGGAATCTTACGTCGTTGATGTTCCTGAAGGTAATACTGAAGAGGATACGCGTCTTTTTGCTGATACCGTTGTGAAGCTCAATCTTCAGAAGCTTGCGTCTGTCACCGAAGGAAAGAACCGTGACGGTGACGGTAAGTCAGATTAG

Protein sequence

>MS.gene054810.t1

MEKAESSTAIASTSDQDSDENHRTQHHLTLPSGLRQHEFDSLIPFINSHHTYLIGPNQCSRLLAQRIHAPPQTVWSVVRSFDKPQIYKHFIKSCSLKEGFQMKVGCTRDVNVISGLPAATSTERLDVLDDERRVTGFSIIGGEHRLKNYRSVTSVHGFGDGDNGGEIWTVVLESYVVDVPEGNTEEDTRLFADTVVKLNLQKLASVTEGKNRDGDGKSD