Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07104.t1 | XP_013450348.1 | 99.6 | 267 | 1 | 0 | 1 | 267 | 200 | 466 | 6.50E-160 | 573.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07104.t1 | O22775 | 88.3 | 266 | 31 | 0 | 1 | 266 | 194 | 459 | 4.4e-149 | 528.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07104.t1 | A0A072U4W6 | 99.6 | 267 | 1 | 0 | 1 | 267 | 200 | 466 | 4.7e-160 | 573.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050903 | MS.gene07104 | 0.813946 | 1.98E-51 | -1.69E-46 |
| MS.gene060811 | MS.gene07104 | 0.801041 | 1.09E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07104.t1 | MTR_7g108675 | 99.625 | 267 | 1 | 0 | 1 | 267 | 200 | 466 | 0.0 | 560 |
| MS.gene07104.t1 | MTR_4g119880 | 90.262 | 267 | 26 | 0 | 1 | 267 | 188 | 454 | 0.0 | 523 |
| MS.gene07104.t1 | MTR_8g058553 | 98.605 | 215 | 3 | 0 | 1 | 215 | 200 | 414 | 5.61e-162 | 460 |
| MS.gene07104.t1 | MTR_4g108940 | 66.415 | 265 | 89 | 0 | 1 | 265 | 184 | 448 | 1.47e-140 | 402 |
| MS.gene07104.t1 | MTR_3g037340 | 66.798 | 253 | 84 | 0 | 1 | 253 | 189 | 441 | 1.26e-136 | 393 |
| MS.gene07104.t1 | MTR_5g015260 | 42.806 | 278 | 124 | 5 | 2 | 244 | 169 | 446 | 2.00e-77 | 241 |
| MS.gene07104.t1 | MTR_4g097240 | 44.318 | 264 | 116 | 3 | 2 | 234 | 163 | 426 | 6.48e-77 | 240 |
| MS.gene07104.t1 | MTR_4g097230 | 44.906 | 265 | 110 | 5 | 7 | 237 | 153 | 415 | 5.81e-75 | 234 |
| MS.gene07104.t1 | MTR_4g108950 | 49.606 | 127 | 20 | 1 | 98 | 224 | 1 | 83 | 4.30e-35 | 121 |
| MS.gene07104.t1 | MTR_4g132910 | 84.211 | 38 | 6 | 0 | 171 | 208 | 583 | 620 | 1.79e-14 | 73.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07104.t1 | AT4G02500 | 88.015 | 267 | 32 | 0 | 1 | 267 | 194 | 460 | 0.0 | 514 |
| MS.gene07104.t1 | AT3G62720 | 85.768 | 267 | 38 | 0 | 1 | 267 | 193 | 459 | 1.23e-178 | 499 |
| MS.gene07104.t1 | AT3G62720 | 85.768 | 267 | 38 | 0 | 1 | 267 | 193 | 459 | 1.23e-178 | 499 |
| MS.gene07104.t1 | AT5G07720 | 63.774 | 265 | 96 | 0 | 1 | 265 | 193 | 457 | 7.71e-136 | 391 |
| MS.gene07104.t1 | AT1G74380 | 66.798 | 253 | 84 | 0 | 1 | 253 | 194 | 446 | 1.13e-135 | 390 |
| MS.gene07104.t1 | AT1G18690 | 66.008 | 253 | 86 | 0 | 1 | 253 | 252 | 504 | 5.41e-134 | 388 |
| MS.gene07104.t1 | AT1G18690 | 66.008 | 253 | 86 | 0 | 1 | 253 | 252 | 504 | 5.41e-134 | 388 |
| MS.gene07104.t1 | AT1G18690 | 66.008 | 253 | 86 | 0 | 1 | 253 | 252 | 504 | 5.41e-134 | 388 |
| MS.gene07104.t1 | AT1G18690 | 66.008 | 253 | 86 | 0 | 1 | 253 | 252 | 504 | 5.41e-134 | 388 |
| MS.gene07104.t1 | AT4G37690 | 40.977 | 266 | 117 | 5 | 2 | 229 | 151 | 414 | 6.61e-67 | 214 |
| MS.gene07104.t1 | AT2G22900 | 40.602 | 266 | 118 | 4 | 2 | 229 | 168 | 431 | 1.67e-66 | 213 |
Find 0 sgRNAs with CRISPR-Local
Find 93 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATCAAATATTGCAGATTTA+TGG | + | 51680:53946-53965 | MS.gene07104:CDS | 20.0% |
| ! | ATGATGAAAAGAATTGGATT+GGG | + | 51680:53500-53519 | MS.gene07104:CDS | 25.0% |
| ! | GAGGTATAAAGATTCAAATT+TGG | + | 51680:53450-53469 | MS.gene07104:CDS | 25.0% |
| ! | TATGATGAAAAGAATTGGAT+TGG | + | 51680:53499-53518 | MS.gene07104:CDS | 25.0% |
| AAAAGAAGCTTCCTAATCAA+TGG | - | 51680:53349-53368 | None:intergenic | 30.0% | |
| ATCTGCTATGGTTTATTTGT+TGG | + | 51680:53681-53700 | MS.gene07104:CDS | 30.0% | |
| ATGATTGAGAATTATCATCC+TGG | + | 51680:53799-53818 | MS.gene07104:CDS | 30.0% | |
| ATGGATAGAGCTTTCAATTT+CGG | + | 51680:53919-53938 | MS.gene07104:CDS | 30.0% | |
| ATTGGATATTCTTGATGCTT+GGG | + | 51680:53564-53583 | MS.gene07104:CDS | 30.0% | |
| GATAAGGTTTATCTTGAGAA+TGG | + | 51680:53727-53746 | MS.gene07104:CDS | 30.0% | |
| TGGTGTATGATGAAAAGAAT+TGG | + | 51680:53494-53513 | MS.gene07104:CDS | 30.0% | |
| TTATTACTTGCATGGTTACT+GGG | + | 51680:53750-53769 | MS.gene07104:CDS | 30.0% | |
| ! | ATTGATTAGGAAGCTTCTTT+TGG | + | 51680:53348-53367 | MS.gene07104:CDS | 30.0% |
| !!! | ATACTGGTAGTTTTTTGTTG+AGG | + | 51680:53527-53546 | MS.gene07104:CDS | 30.0% |
| !!! | GATTTTGGTTGATAGATATG+AGG | + | 51680:53774-53793 | MS.gene07104:CDS | 30.0% |
| AGTTCATCTTTAACCTCAAG+AGG | - | 51680:54033-54052 | None:intergenic | 35.0% | |
| CATTGGATATTCTTGATGCT+TGG | + | 51680:53563-53582 | MS.gene07104:CDS | 35.0% | |
| CTTGAGGTTAAAGATGAACT+TGG | + | 51680:54033-54052 | MS.gene07104:CDS | 35.0% | |
| GAGAATGGTTATTACTTGCA+TGG | + | 51680:53742-53761 | MS.gene07104:CDS | 35.0% | |
| GGATAGTGATGCTATGTTTA+CGG | + | 51680:53402-53421 | MS.gene07104:CDS | 35.0% | |
| GTTATTACTTGCATGGTTAC+TGG | + | 51680:53749-53768 | MS.gene07104:CDS | 35.0% | |
| TATTACTTGCATGGTTACTG+GGG | + | 51680:53751-53770 | MS.gene07104:CDS | 35.0% | |
| TGATGCTATGTTTACGGATA+TGG | + | 51680:53408-53427 | MS.gene07104:CDS | 35.0% | |
| TGGATTCACTCATAAGTCAT+TGG | + | 51680:53966-53985 | MS.gene07104:CDS | 35.0% | |
| ! | CTCATAAGTCATTGGTTAGT+AGG | + | 51680:53974-53993 | MS.gene07104:CDS | 35.0% |
| ! | GAGAATTATCATCCTGGTTT+TGG | + | 51680:53805-53824 | MS.gene07104:CDS | 35.0% |
| ! | TATGGTTTATTTGTTGGCCA+AGG | + | 51680:53687-53706 | MS.gene07104:CDS | 35.0% |
| !! | AATTGGATTGGGTTGAATAC+TGG | + | 51680:53511-53530 | MS.gene07104:CDS | 35.0% |
| !! | ACCTCAACAGCTTTAAAAGT+CGG | - | 51680:54069-54088 | None:intergenic | 35.0% |
| !! | GATTCAAATTTGGTGATGCA+TGG | + | 51680:53460-53479 | MS.gene07104:CDS | 35.0% |
| ACCACAAAAACTCCACTTCT+GGG | - | 51680:53378-53397 | None:intergenic | 40.0% | |
| AGCAGATGATCAATCTGCTA+TGG | + | 51680:53669-53688 | MS.gene07104:CDS | 40.0% | |
| ATTACTTGCATGGTTACTGG+GGG | + | 51680:53752-53771 | MS.gene07104:CDS | 40.0% | |
| GAATCTTTATACCTCTCCCA+CGG | - | 51680:53445-53464 | None:intergenic | 40.0% | |
| GTAGGAGAGTTAGGAGAATA+AGG | + | 51680:53992-54011 | MS.gene07104:CDS | 40.0% | |
| TAATCACCAAACTTCCCACA+AGG | - | 51680:53874-53893 | None:intergenic | 40.0% | |
| TGAGACTAGTAATCCTCTTG+AGG | + | 51680:54017-54036 | MS.gene07104:CDS | 40.0% | |
| TGATCATCTGCTTCGAAAAC+CGG | - | 51680:53661-53680 | None:intergenic | 40.0% | |
| ! | CATTGGTTAGTAGGAGAGTT+AGG | + | 51680:53983-54002 | MS.gene07104:CDS | 40.0% |
| ! | CTTTTTGATGCTGAAATGGC+TGG | + | 51680:53307-53326 | MS.gene07104:CDS | 40.0% |
| ! | GAAGTTTGGTGATTATCCTG+TGG | + | 51680:53879-53898 | MS.gene07104:CDS | 40.0% |
| ! | GGCTCTTTTTGATGCTGAAA+TGG | + | 51680:53303-53322 | MS.gene07104:CDS | 40.0% |
| ! | TTATTTGTTGGCCAAGGAGA+AGG | + | 51680:53693-53712 | MS.gene07104:CDS | 40.0% |
| ! | TTTTGTTGAGGAACTGTCAG+TGG | + | 51680:53539-53558 | MS.gene07104:CDS | 40.0% |
| !! | CAAATTTGGTGATGCATGGT+TGG | + | 51680:53464-53483 | MS.gene07104:CDS | 40.0% |
| !! | CAACAGCTTTAAAAGTCGGA+TGG | - | 51680:54065-54084 | None:intergenic | 40.0% |
| !! | CATTGGTGACTCATTTTGTG+GGG | + | 51680:53842-53861 | MS.gene07104:CDS | 40.0% |
| !! | CCATTGGTGACTCATTTTGT+GGG | + | 51680:53841-53860 | MS.gene07104:CDS | 40.0% |
| !!! | ATGCTGAAATGGCTGGTTTT+TGG | + | 51680:53314-53333 | MS.gene07104:CDS | 40.0% |
| !!! | TCCGACTTTTAAAGCTGTTG+AGG | + | 51680:54065-54084 | MS.gene07104:CDS | 40.0% |
| !!! | TGCTGAAATGGCTGGTTTTT+GGG | + | 51680:53315-53334 | MS.gene07104:CDS | 40.0% |
| CACCACAAAAACTCCACTTC+TGG | - | 51680:53379-53398 | None:intergenic | 45.0% | |
| CCCACAAAATGAGTCACCAA+TGG | - | 51680:53844-53863 | None:intergenic | 45.0% | |
| GAAGGAAAAGTGGGGTGATA+AGG | + | 51680:53711-53730 | MS.gene07104:CDS | 45.0% | |
| GATGCATGGTTGGAATGAGA+TGG | + | 51680:53474-53493 | MS.gene07104:CDS | 45.0% | |
| GGAAAGTTAGGGATGAAGCA+GGG | + | 51680:53605-53624 | MS.gene07104:CDS | 45.0% | |
| TCATCCCTAACTTTCCCCTT+CGG | - | 51680:53601-53620 | None:intergenic | 45.0% | |
| TTACGCGCGAACTCAAAGAT+AGG | + | 51680:53635-53654 | MS.gene07104:CDS | 45.0% | |
| ! | ACCCAGAAGTGGAGTTTTTG+TGG | + | 51680:53374-53393 | MS.gene07104:CDS | 45.0% |
| ! | CAGAAGTGGAGTTTTTGTGG+TGG | + | 51680:53377-53396 | MS.gene07104:CDS | 45.0% |
| ! | TGTAAGCCTTGTGGGAAGTT+TGG | + | 51680:53865-53884 | MS.gene07104:CDS | 45.0% |
| !! | AGTGGAGTTTTTGTGGTGGA+TGG | + | 51680:53381-53400 | MS.gene07104:CDS | 45.0% |
| !! | GCCATTGGTGACTCATTTTG+TGG | + | 51680:53840-53859 | MS.gene07104:CDS | 45.0% |
| !!! | ATATGGCTTTTGAAGTGCCG+TGG | + | 51680:53425-53444 | MS.gene07104:CDS | 45.0% |
| !!! | ATCCTGGTTTTGGTGACCAT+AGG | + | 51680:53815-53834 | MS.gene07104:CDS | 45.0% |
| !!! | TATGGCTTTTGAAGTGCCGT+GGG | + | 51680:53426-53445 | MS.gene07104:CDS | 45.0% |
| CACCTATGGTCACCAAAACC+AGG | - | 51680:53820-53839 | None:intergenic | 50.0% | |
| CTTGATGCTTGGGCACCAAT+GGG | + | 51680:53574-53593 | MS.gene07104:CDS | 50.0% | |
| GAGGAACTGTCAGTGGTCAT+TGG | + | 51680:53546-53565 | MS.gene07104:CDS | 50.0% | |
| GCCAAGGAGAAGGAAAAGTG+GGG | + | 51680:53703-53722 | MS.gene07104:CDS | 50.0% | |
| GGAGAGATGCTTGAAGCAGA+TGG | + | 51680:53900-53919 | MS.gene07104:CDS | 50.0% | |
| GGCCAAGGAGAAGGAAAAGT+GGG | + | 51680:53702-53721 | MS.gene07104:CDS | 50.0% | |
| GGGAAAGTTAGGGATGAAGC+AGG | + | 51680:53604-53623 | MS.gene07104:CDS | 50.0% | |
| TCTTGATGCTTGGGCACCAA+TGG | + | 51680:53573-53592 | MS.gene07104:CDS | 50.0% | |
| TGCTTCAAGCATCTCTCCAC+AGG | - | 51680:53898-53917 | None:intergenic | 50.0% | |
| TGGCCAAGGAGAAGGAAAAG+TGG | + | 51680:53701-53720 | MS.gene07104:CDS | 50.0% | |
| TTTGTGGGGTGTAAGCCTTG+TGG | + | 51680:53856-53875 | MS.gene07104:CDS | 50.0% | |
| ! | GCATGGTTACTGGGGGATTT+TGG | + | 51680:53759-53778 | MS.gene07104:CDS | 50.0% |
| ! | GGGCGAAGTTGCCATTGATT+AGG | + | 51680:53335-53354 | MS.gene07104:CDS | 50.0% |
| ! | TCTTTTGGCACACCCAGAAG+TGG | + | 51680:53363-53382 | MS.gene07104:CDS | 50.0% |
| ! | TTGTGGGGTGTAAGCCTTGT+GGG | + | 51680:53857-53876 | MS.gene07104:CDS | 50.0% |
| !! | ACCCCACTTTTCCTTCTCCT+TGG | - | 51680:53707-53726 | None:intergenic | 50.0% |
| !!! | CTGGTTTTGGTGACCATAGG+TGG | + | 51680:53818-53837 | MS.gene07104:CDS | 50.0% |
| ACTTTCCCCTTCGGTCCCAT+TGG | - | 51680:53592-53611 | None:intergenic | 55.0% | |
| GAGTCACCAATGGCCACCTA+TGG | - | 51680:53834-53853 | None:intergenic | 55.0% | |
| GCGCGAACTCAAAGATAGGC+CGG | + | 51680:53639-53658 | MS.gene07104:CDS | 55.0% | |
| GGGACCGAAGGGGAAAGTTA+GGG | + | 51680:53594-53613 | MS.gene07104:CDS | 55.0% | |
| TGGGACCGAAGGGGAAAGTT+AGG | + | 51680:53593-53612 | MS.gene07104:CDS | 55.0% | |
| TGGTGACCATAGGTGGCCAT+TGG | + | 51680:53825-53844 | MS.gene07104:CDS | 55.0% | |
| !!! | CTTTTGAAGTGCCGTGGGAG+AGG | + | 51680:53431-53450 | MS.gene07104:CDS | 55.0% |
| TGGGCACCAATGGGACCGAA+GGG | + | 51680:53583-53602 | MS.gene07104:CDS | 60.0% | |
| TTGGGCACCAATGGGACCGA+AGG | + | 51680:53582-53601 | MS.gene07104:CDS | 60.0% | |
| GGGCACCAATGGGACCGAAG+GGG | + | 51680:53584-53603 | MS.gene07104:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 51680 | gene | 53301 | 54104 | 53301 | ID=MS.gene07104 |
| 51680 | mRNA | 53301 | 54104 | 53301 | ID=MS.gene07104.t1;Parent=MS.gene07104 |
| 51680 | exon | 53301 | 54104 | 53301 | ID=MS.gene07104.t1.exon1;Parent=MS.gene07104.t1 |
| 51680 | CDS | 53301 | 54104 | 53301 | ID=cds.MS.gene07104.t1;Parent=MS.gene07104.t1 |
| Gene Sequence |
| Protein sequence |
