Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07105.t1 | XP_013450348.1 | 99.6 | 267 | 1 | 0 | 1 | 267 | 200 | 466 | 6.50E-160 | 573.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07105.t1 | O22775 | 88.3 | 266 | 31 | 0 | 1 | 266 | 194 | 459 | 4.4e-149 | 528.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07105.t1 | A0A072U4W6 | 99.6 | 267 | 1 | 0 | 1 | 267 | 200 | 466 | 4.7e-160 | 573.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050903 | MS.gene07105 | 0.81681 | 4.56E-52 | -1.69E-46 |
| MS.gene060811 | MS.gene07105 | 0.801463 | 8.90E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07105.t1 | MTR_7g108675 | 99.625 | 267 | 1 | 0 | 1 | 267 | 200 | 466 | 0.0 | 560 |
| MS.gene07105.t1 | MTR_4g119880 | 90.262 | 267 | 26 | 0 | 1 | 267 | 188 | 454 | 0.0 | 523 |
| MS.gene07105.t1 | MTR_8g058553 | 98.605 | 215 | 3 | 0 | 1 | 215 | 200 | 414 | 5.61e-162 | 460 |
| MS.gene07105.t1 | MTR_4g108940 | 66.415 | 265 | 89 | 0 | 1 | 265 | 184 | 448 | 1.47e-140 | 402 |
| MS.gene07105.t1 | MTR_3g037340 | 66.798 | 253 | 84 | 0 | 1 | 253 | 189 | 441 | 1.26e-136 | 393 |
| MS.gene07105.t1 | MTR_5g015260 | 42.806 | 278 | 124 | 5 | 2 | 244 | 169 | 446 | 2.00e-77 | 241 |
| MS.gene07105.t1 | MTR_4g097240 | 44.318 | 264 | 116 | 3 | 2 | 234 | 163 | 426 | 6.48e-77 | 240 |
| MS.gene07105.t1 | MTR_4g097230 | 44.906 | 265 | 110 | 5 | 7 | 237 | 153 | 415 | 5.81e-75 | 234 |
| MS.gene07105.t1 | MTR_4g108950 | 49.606 | 127 | 20 | 1 | 98 | 224 | 1 | 83 | 4.30e-35 | 121 |
| MS.gene07105.t1 | MTR_4g132910 | 84.211 | 38 | 6 | 0 | 171 | 208 | 583 | 620 | 1.79e-14 | 73.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07105.t1 | AT4G02500 | 88.015 | 267 | 32 | 0 | 1 | 267 | 194 | 460 | 0.0 | 514 |
| MS.gene07105.t1 | AT3G62720 | 85.768 | 267 | 38 | 0 | 1 | 267 | 193 | 459 | 1.23e-178 | 499 |
| MS.gene07105.t1 | AT3G62720 | 85.768 | 267 | 38 | 0 | 1 | 267 | 193 | 459 | 1.23e-178 | 499 |
| MS.gene07105.t1 | AT5G07720 | 63.774 | 265 | 96 | 0 | 1 | 265 | 193 | 457 | 7.71e-136 | 391 |
| MS.gene07105.t1 | AT1G74380 | 66.798 | 253 | 84 | 0 | 1 | 253 | 194 | 446 | 1.13e-135 | 390 |
| MS.gene07105.t1 | AT1G18690 | 66.008 | 253 | 86 | 0 | 1 | 253 | 252 | 504 | 5.41e-134 | 388 |
| MS.gene07105.t1 | AT1G18690 | 66.008 | 253 | 86 | 0 | 1 | 253 | 252 | 504 | 5.41e-134 | 388 |
| MS.gene07105.t1 | AT1G18690 | 66.008 | 253 | 86 | 0 | 1 | 253 | 252 | 504 | 5.41e-134 | 388 |
| MS.gene07105.t1 | AT1G18690 | 66.008 | 253 | 86 | 0 | 1 | 253 | 252 | 504 | 5.41e-134 | 388 |
| MS.gene07105.t1 | AT4G37690 | 40.977 | 266 | 117 | 5 | 2 | 229 | 151 | 414 | 6.61e-67 | 214 |
| MS.gene07105.t1 | AT2G22900 | 40.602 | 266 | 118 | 4 | 2 | 229 | 168 | 431 | 1.67e-66 | 213 |
Find 0 sgRNAs with CRISPR-Local
Find 93 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATCAAATATTGCAGATTTA+TGG | + | 51686:6009-6028 | MS.gene07105:CDS | 20.0% |
| ! | ATGATGAAAAGAATTGGATT+GGG | + | 51686:5563-5582 | MS.gene07105:CDS | 25.0% |
| ! | GAGGTATAAAGATTCAAATT+TGG | + | 51686:5513-5532 | MS.gene07105:CDS | 25.0% |
| ! | TATGATGAAAAGAATTGGAT+TGG | + | 51686:5562-5581 | MS.gene07105:CDS | 25.0% |
| AAAAGAAGCTTCCTAATCAA+TGG | - | 51686:5412-5431 | None:intergenic | 30.0% | |
| ATCTGCTATGGTTTATTTGT+TGG | + | 51686:5744-5763 | MS.gene07105:CDS | 30.0% | |
| ATGATTGAGAATTATCATCC+TGG | + | 51686:5862-5881 | MS.gene07105:CDS | 30.0% | |
| ATGGATAGAGCTTTCAATTT+CGG | + | 51686:5982-6001 | MS.gene07105:CDS | 30.0% | |
| ATTGGATATTCTTGATGCTT+GGG | + | 51686:5627-5646 | MS.gene07105:CDS | 30.0% | |
| GATAAGGTTTATCTTGAGAA+TGG | + | 51686:5790-5809 | MS.gene07105:CDS | 30.0% | |
| TGGTGTATGATGAAAAGAAT+TGG | + | 51686:5557-5576 | MS.gene07105:CDS | 30.0% | |
| TTATTACTTGCATGGTTACT+GGG | + | 51686:5813-5832 | MS.gene07105:CDS | 30.0% | |
| ! | ATTGATTAGGAAGCTTCTTT+TGG | + | 51686:5411-5430 | MS.gene07105:CDS | 30.0% |
| !!! | ATACTGGTAGTTTTTTGTTG+AGG | + | 51686:5590-5609 | MS.gene07105:CDS | 30.0% |
| !!! | GATTTTGGTTGATAGATATG+AGG | + | 51686:5837-5856 | MS.gene07105:CDS | 30.0% |
| AGTTCATCTTTAACCTCAAG+AGG | - | 51686:6096-6115 | MS.gene07105:intergenic | 35.0% | |
| CATTGGATATTCTTGATGCT+TGG | + | 51686:5626-5645 | MS.gene07105:CDS | 35.0% | |
| CTTGAGGTTAAAGATGAACT+TGG | + | 51686:6096-6115 | MS.gene07105:CDS | 35.0% | |
| GAGAATGGTTATTACTTGCA+TGG | + | 51686:5805-5824 | MS.gene07105:CDS | 35.0% | |
| GGATAGTGATGCTATGTTTA+CGG | + | 51686:5465-5484 | MS.gene07105:CDS | 35.0% | |
| GTTATTACTTGCATGGTTAC+TGG | + | 51686:5812-5831 | MS.gene07105:CDS | 35.0% | |
| TATTACTTGCATGGTTACTG+GGG | + | 51686:5814-5833 | MS.gene07105:CDS | 35.0% | |
| TGATGCTATGTTTACGGATA+TGG | + | 51686:5471-5490 | MS.gene07105:CDS | 35.0% | |
| TGGATTCACTCATAAGTCAT+TGG | + | 51686:6029-6048 | MS.gene07105:CDS | 35.0% | |
| ! | CTCATAAGTCATTGGTTAGT+AGG | + | 51686:6037-6056 | MS.gene07105:CDS | 35.0% |
| ! | GAGAATTATCATCCTGGTTT+TGG | + | 51686:5868-5887 | MS.gene07105:CDS | 35.0% |
| ! | TATGGTTTATTTGTTGGCCA+AGG | + | 51686:5750-5769 | MS.gene07105:CDS | 35.0% |
| !! | AATTGGATTGGGTTGAATAC+TGG | + | 51686:5574-5593 | MS.gene07105:CDS | 35.0% |
| !! | ACCTCAACAGCTTTAAAAGT+CGG | - | 51686:6132-6151 | MS.gene07105:intergenic | 35.0% |
| !! | GATTCAAATTTGGTGATGCA+TGG | + | 51686:5523-5542 | MS.gene07105:CDS | 35.0% |
| ACCACAAAAACTCCACTTCT+GGG | - | 51686:5441-5460 | None:intergenic | 40.0% | |
| AGCAGATGATCAATCTGCTA+TGG | + | 51686:5732-5751 | MS.gene07105:CDS | 40.0% | |
| ATTACTTGCATGGTTACTGG+GGG | + | 51686:5815-5834 | MS.gene07105:CDS | 40.0% | |
| GAATCTTTATACCTCTCCCA+CGG | - | 51686:5508-5527 | MS.gene07105:intergenic | 40.0% | |
| GTAGGAGAGTTAGGAGAATA+AGG | + | 51686:6055-6074 | MS.gene07105:CDS | 40.0% | |
| TAATCACCAAACTTCCCACA+AGG | - | 51686:5937-5956 | MS.gene07105:intergenic | 40.0% | |
| TGAGACTAGTAATCCTCTTG+AGG | + | 51686:6080-6099 | MS.gene07105:CDS | 40.0% | |
| TGATCATCTGCTTCGAAAAC+CGG | - | 51686:5724-5743 | MS.gene07105:intergenic | 40.0% | |
| ! | CATTGGTTAGTAGGAGAGTT+AGG | + | 51686:6046-6065 | MS.gene07105:CDS | 40.0% |
| ! | CTTTTTGATGCTGAAATGGC+TGG | + | 51686:5370-5389 | MS.gene07105:CDS | 40.0% |
| ! | GAAGTTTGGTGATTATCCTG+TGG | + | 51686:5942-5961 | MS.gene07105:CDS | 40.0% |
| ! | GGCTCTTTTTGATGCTGAAA+TGG | + | 51686:5366-5385 | MS.gene07105:CDS | 40.0% |
| ! | TTATTTGTTGGCCAAGGAGA+AGG | + | 51686:5756-5775 | MS.gene07105:CDS | 40.0% |
| ! | TTTTGTTGAGGAACTGTCAG+TGG | + | 51686:5602-5621 | MS.gene07105:CDS | 40.0% |
| !! | CAAATTTGGTGATGCATGGT+TGG | + | 51686:5527-5546 | MS.gene07105:CDS | 40.0% |
| !! | CAACAGCTTTAAAAGTCGGA+TGG | - | 51686:6128-6147 | MS.gene07105:intergenic | 40.0% |
| !! | CATTGGTGACTCATTTTGTG+GGG | + | 51686:5905-5924 | MS.gene07105:CDS | 40.0% |
| !! | CCATTGGTGACTCATTTTGT+GGG | + | 51686:5904-5923 | MS.gene07105:CDS | 40.0% |
| !!! | ATGCTGAAATGGCTGGTTTT+TGG | + | 51686:5377-5396 | MS.gene07105:CDS | 40.0% |
| !!! | TCCGACTTTTAAAGCTGTTG+AGG | + | 51686:6128-6147 | MS.gene07105:CDS | 40.0% |
| !!! | TGCTGAAATGGCTGGTTTTT+GGG | + | 51686:5378-5397 | MS.gene07105:CDS | 40.0% |
| CACCACAAAAACTCCACTTC+TGG | - | 51686:5442-5461 | MS.gene07105:intergenic | 45.0% | |
| CCCACAAAATGAGTCACCAA+TGG | - | 51686:5907-5926 | MS.gene07105:intergenic | 45.0% | |
| GAAGGAAAAGTGGGGTGATA+AGG | + | 51686:5774-5793 | MS.gene07105:CDS | 45.0% | |
| GATGCATGGTTGGAATGAGA+TGG | + | 51686:5537-5556 | MS.gene07105:CDS | 45.0% | |
| GGAAAGTTAGGGATGAAGCA+GGG | + | 51686:5668-5687 | MS.gene07105:CDS | 45.0% | |
| TCATCCCTAACTTTCCCCTT+CGG | - | 51686:5664-5683 | MS.gene07105:intergenic | 45.0% | |
| TTACGCGCGAACTCAAAGAT+AGG | + | 51686:5698-5717 | MS.gene07105:CDS | 45.0% | |
| ! | ACCCAGAAGTGGAGTTTTTG+TGG | + | 51686:5437-5456 | MS.gene07105:CDS | 45.0% |
| ! | CAGAAGTGGAGTTTTTGTGG+TGG | + | 51686:5440-5459 | MS.gene07105:CDS | 45.0% |
| ! | TGTAAGCCTTGTGGGAAGTT+TGG | + | 51686:5928-5947 | MS.gene07105:CDS | 45.0% |
| !! | AGTGGAGTTTTTGTGGTGGA+TGG | + | 51686:5444-5463 | MS.gene07105:CDS | 45.0% |
| !! | GCCATTGGTGACTCATTTTG+TGG | + | 51686:5903-5922 | MS.gene07105:CDS | 45.0% |
| !!! | ATATGGCTTTTGAAGTGCCG+TGG | + | 51686:5488-5507 | MS.gene07105:CDS | 45.0% |
| !!! | ATCCTGGTTTTGGTGACCAT+AGG | + | 51686:5878-5897 | MS.gene07105:CDS | 45.0% |
| !!! | TATGGCTTTTGAAGTGCCGT+GGG | + | 51686:5489-5508 | MS.gene07105:CDS | 45.0% |
| CACCTATGGTCACCAAAACC+AGG | - | 51686:5883-5902 | MS.gene07105:intergenic | 50.0% | |
| CTTGATGCTTGGGCACCAAT+GGG | + | 51686:5637-5656 | MS.gene07105:CDS | 50.0% | |
| GAGGAACTGTCAGTGGTCAT+TGG | + | 51686:5609-5628 | MS.gene07105:CDS | 50.0% | |
| GCCAAGGAGAAGGAAAAGTG+GGG | + | 51686:5766-5785 | MS.gene07105:CDS | 50.0% | |
| GGAGAGATGCTTGAAGCAGA+TGG | + | 51686:5963-5982 | MS.gene07105:CDS | 50.0% | |
| GGCCAAGGAGAAGGAAAAGT+GGG | + | 51686:5765-5784 | MS.gene07105:CDS | 50.0% | |
| GGGAAAGTTAGGGATGAAGC+AGG | + | 51686:5667-5686 | MS.gene07105:CDS | 50.0% | |
| TCTTGATGCTTGGGCACCAA+TGG | + | 51686:5636-5655 | MS.gene07105:CDS | 50.0% | |
| TGCTTCAAGCATCTCTCCAC+AGG | - | 51686:5961-5980 | MS.gene07105:intergenic | 50.0% | |
| TGGCCAAGGAGAAGGAAAAG+TGG | + | 51686:5764-5783 | MS.gene07105:CDS | 50.0% | |
| TTTGTGGGGTGTAAGCCTTG+TGG | + | 51686:5919-5938 | MS.gene07105:CDS | 50.0% | |
| ! | GCATGGTTACTGGGGGATTT+TGG | + | 51686:5822-5841 | MS.gene07105:CDS | 50.0% |
| ! | GGGCGAAGTTGCCATTGATT+AGG | + | 51686:5398-5417 | MS.gene07105:CDS | 50.0% |
| ! | TCTTTTGGCACACCCAGAAG+TGG | + | 51686:5426-5445 | MS.gene07105:CDS | 50.0% |
| ! | TTGTGGGGTGTAAGCCTTGT+GGG | + | 51686:5920-5939 | MS.gene07105:CDS | 50.0% |
| !! | ACCCCACTTTTCCTTCTCCT+TGG | - | 51686:5770-5789 | MS.gene07105:intergenic | 50.0% |
| !!! | CTGGTTTTGGTGACCATAGG+TGG | + | 51686:5881-5900 | MS.gene07105:CDS | 50.0% |
| ACTTTCCCCTTCGGTCCCAT+TGG | - | 51686:5655-5674 | None:intergenic | 55.0% | |
| GAGTCACCAATGGCCACCTA+TGG | - | 51686:5897-5916 | MS.gene07105:intergenic | 55.0% | |
| GCGCGAACTCAAAGATAGGC+CGG | + | 51686:5702-5721 | MS.gene07105:CDS | 55.0% | |
| GGGACCGAAGGGGAAAGTTA+GGG | + | 51686:5657-5676 | MS.gene07105:CDS | 55.0% | |
| TGGGACCGAAGGGGAAAGTT+AGG | + | 51686:5656-5675 | MS.gene07105:CDS | 55.0% | |
| TGGTGACCATAGGTGGCCAT+TGG | + | 51686:5888-5907 | MS.gene07105:CDS | 55.0% | |
| !!! | CTTTTGAAGTGCCGTGGGAG+AGG | + | 51686:5494-5513 | MS.gene07105:CDS | 55.0% |
| TGGGCACCAATGGGACCGAA+GGG | + | 51686:5646-5665 | MS.gene07105:CDS | 60.0% | |
| TTGGGCACCAATGGGACCGA+AGG | + | 51686:5645-5664 | MS.gene07105:CDS | 60.0% | |
| GGGCACCAATGGGACCGAAG+GGG | + | 51686:5647-5666 | MS.gene07105:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 51686 | gene | 5364 | 6167 | 5364 | ID=MS.gene07105 |
| 51686 | mRNA | 5364 | 6167 | 5364 | ID=MS.gene07105.t1;Parent=MS.gene07105 |
| 51686 | exon | 5364 | 6167 | 5364 | ID=MS.gene07105.t1.exon1;Parent=MS.gene07105.t1 |
| 51686 | CDS | 5364 | 6167 | 5364 | ID=cds.MS.gene07105.t1;Parent=MS.gene07105.t1 |
| Gene Sequence |
| Protein sequence |
