Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071061.t1 | TKY65880.1 | 84 | 50 | 7 | 1 | 1 | 50 | 1 | 49 | 1.50E-09 | 72 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071061.t1 | A0A0L9VFF0 | 56.3 | 71 | 29 | 2 | 1 | 70 | 1 | 70 | 1.1e-06 | 62.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051199 | MS.gene071061 | 0.808626 | 2.82E-50 | -1.69E-46 |
| MS.gene051719 | MS.gene071061 | 0.814239 | 1.70E-51 | -1.69E-46 |
| MS.gene051720 | MS.gene071061 | 0.809159 | 2.17E-50 | -1.69E-46 |
| MS.gene053974 | MS.gene071061 | 0.815471 | 9.09E-52 | -1.69E-46 |
| MS.gene054801 | MS.gene071061 | 0.806816 | 6.84E-50 | -1.69E-46 |
| MS.gene058515 | MS.gene071061 | 0.825427 | 4.72E-54 | -1.69E-46 |
| MS.gene059015 | MS.gene071061 | 0.800939 | 1.14E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 22 sgRNAs with CRISPR-Local
Find 38 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCAGAGTTGGAATGTTTGTT+AGG | 0.274254 | 7.3:+37626793 | MS.gene071061:CDS |
| GGTTTCTTCTTCATCTACTT+TGG | 0.392407 | 7.3:-37626441 | None:intergenic |
| CTTCTTCATCTACTTTGGTA+AGG | 0.422908 | 7.3:-37626436 | None:intergenic |
| CAGAGTTGGAATGTTTGTTA+GGG | 0.422995 | 7.3:+37626794 | MS.gene071061:CDS |
| TGTTTGTTAGGGAGGATAGT+TGG | 0.426639 | 7.3:+37626805 | MS.gene071061:CDS |
| AGAATATGCAAAAGGGAGAT+AGG | 0.429184 | 7.3:+37626761 | MS.gene071061:CDS |
| AGAGAAACAGAATATGCAAA+AGG | 0.440819 | 7.3:+37626753 | MS.gene071061:CDS |
| TAGGTTTCAAGAGGCAGAGT+TGG | 0.456082 | 7.3:+37626780 | MS.gene071061:CDS |
| AGATGAAGAAGAAACCTTCC+TGG | 0.456566 | 7.3:+37626448 | MS.gene071061:CDS |
| AGTTGGAATGTTTGTTAGGG+AGG | 0.457709 | 7.3:+37626797 | MS.gene071061:CDS |
| AGTTTCAGCTCGATGTGAAT+CGG | 0.520615 | 7.3:+37626391 | MS.gene071061:CDS |
| TGTTAGGGAGGATAGTTGGA+TGG | 0.531829 | 7.3:+37626809 | MS.gene071061:CDS |
| GAGAAACAGAATATGCAAAA+GGG | 0.536098 | 7.3:+37626754 | MS.gene071061:CDS |
| TTGAAATACCTAGTGCAACT+AGG | 0.546594 | 7.3:-37626521 | None:intergenic |
| CTATGAAGAGAAGATCCTCC+AGG | 0.550337 | 7.3:-37626466 | None:intergenic |
| TACTTTGGTAAGGTCACGAT+CGG | 0.574594 | 7.3:-37626426 | None:intergenic |
| AGTTGCACTAGGTATTTCAA+AGG | 0.604433 | 7.3:+37626524 | MS.gene071061:CDS |
| AAAGGGAGATAGGTTTCAAG+AGG | 0.619008 | 7.3:+37626771 | MS.gene071061:CDS |
| AGTGCAACTAGGTACAAAAG+TGG | 0.619466 | 7.3:-37626510 | None:intergenic |
| GAAGAGAAGATCCTCCAGGA+AGG | 0.654806 | 7.3:-37626462 | None:intergenic |
| CAGCTCGATGTGAATCGGTG+AGG | 0.671793 | 7.3:+37626396 | MS.gene071061:CDS |
| TGAAGAAGAAACCTTCCTGG+AGG | 0.701035 | 7.3:+37626451 | MS.gene071061:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | GTTATTTTAGTTTTTGTGTT+TGG | + | chr7.3:37626717-37626736 | MS.gene071061:intron | 20.0% |
| ! | AAATGTCCATTAAAAGCAAA+AGG | - | chr7.3:37626658-37626677 | None:intergenic | 25.0% |
| ! | AATGTCCATTAAAAGCAAAA+GGG | - | chr7.3:37626657-37626676 | None:intergenic | 25.0% |
| ! | ACTATAGGAGAATTCTAATT+TGG | + | chr7.3:37626684-37626703 | MS.gene071061:intron | 25.0% |
| !! | AACAATTTTTCTCAAGAAGA+GGG | - | chr7.3:37626603-37626622 | None:intergenic | 25.0% |
| AGAGAAACAGAATATGCAAA+AGG | + | chr7.3:37626753-37626772 | MS.gene071061:CDS | 30.0% | |
| CTCTTCTTGAGAAAAATTGT+TGG | + | chr7.3:37626602-37626621 | MS.gene071061:intron | 30.0% | |
| GAGAAACAGAATATGCAAAA+GGG | + | chr7.3:37626754-37626773 | MS.gene071061:CDS | 30.0% | |
| TGACAATCTTGATTGTTCTT+TGG | + | chr7.3:37626550-37626569 | MS.gene071061:intron | 30.0% | |
| ! | CAACAATTTTTCTCAAGAAG+AGG | - | chr7.3:37626604-37626623 | None:intergenic | 30.0% |
| !! | TGGACATTTTGAAGAACTAT+AGG | + | chr7.3:37626669-37626688 | MS.gene071061:intron | 30.0% |
| !!! | TTTTGTGTTTGGTTTGTGAA+GGG | + | chr7.3:37626728-37626747 | MS.gene071061:intron | 30.0% |
| !!! | TTTTTGTGTTTGGTTTGTGA+AGG | + | chr7.3:37626727-37626746 | MS.gene071061:intron | 30.0% |
| AAGAAGAGGGAAATTGGAAA+TGG | - | chr7.3:37626590-37626609 | None:intergenic | 35.0% | |
| AGAATATGCAAAAGGGAGAT+AGG | + | chr7.3:37626761-37626780 | MS.gene071061:CDS | 35.0% | |
| AGTTGCACTAGGTATTTCAA+AGG | + | chr7.3:37626524-37626543 | MS.gene071061:CDS | 35.0% | |
| CTTCTTCATCTACTTTGGTA+AGG | - | chr7.3:37626439-37626458 | None:intergenic | 35.0% | |
| GGTTTCTTCTTCATCTACTT+TGG | - | chr7.3:37626444-37626463 | None:intergenic | 35.0% | |
| GTTGGTATGTCATTGACATT+AGG | + | chr7.3:37626620-37626639 | MS.gene071061:intron | 35.0% | |
| TTGAAATACCTAGTGCAACT+AGG | - | chr7.3:37626524-37626543 | None:intergenic | 35.0% | |
| TTTCTCAAGAAGAGGGAAAT+TGG | - | chr7.3:37626596-37626615 | None:intergenic | 35.0% | |
| ! | CAGAGTTGGAATGTTTGTTA+GGG | + | chr7.3:37626794-37626813 | MS.gene071061:CDS | 35.0% |
| !! | GCATACCCTTTTGCTTTTAA+TGG | + | chr7.3:37626649-37626668 | MS.gene071061:intron | 35.0% |
| AAAGGGAGATAGGTTTCAAG+AGG | + | chr7.3:37626771-37626790 | MS.gene071061:CDS | 40.0% | |
| AGATGAAGAAGAAACCTTCC+TGG | + | chr7.3:37626448-37626467 | MS.gene071061:CDS | 40.0% | |
| AGTGCAACTAGGTACAAAAG+TGG | - | chr7.3:37626513-37626532 | None:intergenic | 40.0% | |
| AGTTTCAGCTCGATGTGAAT+CGG | + | chr7.3:37626391-37626410 | MS.gene071061:CDS | 40.0% | |
| GCAGAGTTGGAATGTTTGTT+AGG | + | chr7.3:37626793-37626812 | MS.gene071061:CDS | 40.0% | |
| TACTTTGGTAAGGTCACGAT+CGG | - | chr7.3:37626429-37626448 | None:intergenic | 40.0% | |
| ! | AGTTGGAATGTTTGTTAGGG+AGG | + | chr7.3:37626797-37626816 | MS.gene071061:CDS | 40.0% |
| ! | CTTTTGTACCTAGTTGCACT+AGG | + | chr7.3:37626513-37626532 | MS.gene071061:CDS | 40.0% |
| ! | TGTTTGTTAGGGAGGATAGT+TGG | + | chr7.3:37626805-37626824 | MS.gene071061:CDS | 40.0% |
| CTATGAAGAGAAGATCCTCC+AGG | - | chr7.3:37626469-37626488 | None:intergenic | 45.0% | |
| TAGGTTTCAAGAGGCAGAGT+TGG | + | chr7.3:37626780-37626799 | MS.gene071061:CDS | 45.0% | |
| TGAAGAAGAAACCTTCCTGG+AGG | + | chr7.3:37626451-37626470 | MS.gene071061:CDS | 45.0% | |
| TGTTAGGGAGGATAGTTGGA+TGG | + | chr7.3:37626809-37626828 | MS.gene071061:CDS | 45.0% | |
| GAAGAGAAGATCCTCCAGGA+AGG | - | chr7.3:37626465-37626484 | None:intergenic | 50.0% | |
| CAGCTCGATGTGAATCGGTG+AGG | + | chr7.3:37626396-37626415 | MS.gene071061:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 37626377 | 37626846 | 37626377 | ID=MS.gene071061 |
| chr7.3 | mRNA | 37626377 | 37626846 | 37626377 | ID=MS.gene071061.t1;Parent=MS.gene071061 |
| chr7.3 | exon | 37626377 | 37626545 | 37626377 | ID=MS.gene071061.t1.exon1;Parent=MS.gene071061.t1 |
| chr7.3 | CDS | 37626377 | 37626545 | 37626377 | ID=cds.MS.gene071061.t1;Parent=MS.gene071061.t1 |
| chr7.3 | exon | 37626749 | 37626846 | 37626749 | ID=MS.gene071061.t1.exon2;Parent=MS.gene071061.t1 |
| chr7.3 | CDS | 37626749 | 37626846 | 37626749 | ID=cds.MS.gene071061.t1;Parent=MS.gene071061.t1 |
| Gene Sequence |
| Protein sequence |
