AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene071232

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene071232.t1 MTR_5g083040 95.098 204 10 0 1 204 1 204 4.08e-121 347
MS.gene071232.t1 MTR_5g074270 41.327 196 114 1 10 204 11 206 2.26e-51 169
MS.gene071232.t1 MTR_4g087985 45.251 179 95 2 28 204 32 209 3.94e-49 164
MS.gene071232.t1 MTR_3g464730 39.086 197 114 2 10 204 10 202 1.12e-48 162
MS.gene071232.t1 MTR_5g084750 40.816 196 112 3 11 204 10 203 1.63e-48 162
MS.gene071232.t1 MTR_3g114120 43.750 176 96 2 31 204 46 220 2.03e-48 162
MS.gene071232.t1 MTR_1g025780 40.571 175 104 0 30 204 39 213 5.31e-48 160
MS.gene071232.t1 MTR_2g015080 41.709 199 107 5 12 204 19 214 5.61e-48 161
MS.gene071232.t1 MTR_5g084770 41.011 178 104 1 28 204 24 201 2.23e-47 159
MS.gene071232.t1 MTR_3g114090 44.318 176 96 2 31 204 35 210 4.02e-47 158
MS.gene071232.t1 MTR_1g025700 40.000 185 105 2 21 204 44 223 5.75e-47 158
MS.gene071232.t1 MTR_8g075230 40.526 190 108 2 16 204 24 209 6.87e-47 157
MS.gene071232.t1 MTR_1g025760 39.801 201 115 2 10 204 54 254 9.42e-47 158
MS.gene071232.t1 MTR_1g025680 37.745 204 121 3 7 204 5 208 4.23e-45 155
MS.gene071232.t1 MTR_3g082830 42.541 181 95 2 29 204 35 211 2.07e-44 151
MS.gene071232.t1 MTR_1g025720 36.816 201 120 3 10 204 11 210 6.90e-44 150
MS.gene071232.t1 MTR_5g022640 40.838 191 105 4 17 204 32 217 9.82e-44 150
MS.gene071232.t1 MTR_8g075200 40.571 175 103 1 31 204 40 214 4.81e-43 149
MS.gene071232.t1 MTR_5g022630 41.538 195 99 6 17 204 32 218 3.31e-42 145
MS.gene071232.t1 MTR_1g025910 48.966 145 73 1 37 180 41 185 1.21e-41 144
MS.gene071232.t1 MTR_5g084790 40.331 181 99 2 29 204 29 205 1.65e-41 144
MS.gene071232.t1 MTR_8g075220 43.137 153 83 1 31 179 39 191 1.05e-40 142
MS.gene071232.t1 MTR_8g014910 36.000 175 111 1 31 204 31 205 1.41e-39 139
MS.gene071232.t1 MTR_2g100620 40.667 150 89 0 30 179 29 178 7.01e-37 131
MS.gene071232.t1 MTR_4g056160 36.723 177 109 2 30 204 25 200 8.16e-35 126
MS.gene071232.t1 MTR_2g084585 33.816 207 120 2 7 204 5 203 8.42e-34 123
MS.gene071232.t1 MTR_4g056280 34.146 205 120 4 11 204 3 203 3.78e-33 122
MS.gene071232.t1 MTR_4g036375 35.385 195 117 2 11 199 3 194 7.84e-33 117
MS.gene071232.t1 MTR_1g079590 34.615 208 124 5 6 204 2 206 1.06e-32 117
MS.gene071232.t1 MTR_7g116570 36.082 194 118 3 7 195 5 197 1.52e-32 120
MS.gene071232.t1 MTR_1g069175 40.000 150 89 1 32 180 33 182 1.55e-30 115
MS.gene071232.t1 MTR_1g079530 35.196 179 112 3 8 183 3 180 5.15e-30 114
MS.gene071232.t1 MTR_7g116520 32.571 175 116 2 7 180 9 182 5.78e-29 111
MS.gene071232.t1 MTR_5g009100 38.462 143 86 2 34 175 48 189 4.38e-28 108
MS.gene071232.t1 MTR_7g116510 33.526 173 113 2 24 195 17 188 5.66e-28 108
MS.gene071232.t1 MTR_2g044100 38.012 171 93 5 12 174 17 182 6.74e-28 108
MS.gene071232.t1 MTR_2g017915 33.333 177 110 5 28 199 29 202 7.20e-28 107
MS.gene071232.t1 MTR_2g017925 31.220 205 132 6 1 199 1 202 9.28e-28 107
MS.gene071232.t1 MTR_1g079540 34.463 177 111 3 32 204 31 206 1.34e-27 107
MS.gene071232.t1 MTR_7g116500 33.125 160 105 2 22 180 23 181 1.98e-27 107
MS.gene071232.t1 MTR_2g017955 34.320 169 104 5 29 194 30 194 4.62e-27 106
MS.gene071232.t1 MTR_1g024890 33.163 196 129 2 1 195 1 195 7.21e-27 105
MS.gene071232.t1 MTR_6g021530 32.386 176 113 4 34 204 31 205 1.14e-26 105
MS.gene071232.t1 MTR_8g074570 37.853 177 100 4 6 174 12 186 8.17e-26 102
MS.gene071232.t1 MTR_8g087870 28.643 199 135 4 10 204 7 202 1.56e-25 101
MS.gene071232.t1 MTR_2g017875 32.609 184 116 5 27 204 29 210 2.52e-25 101
MS.gene071232.t1 MTR_2g033930 34.940 166 104 3 10 174 10 172 4.58e-25 100
MS.gene071232.t1 MTR_8g087890 34.839 155 95 4 29 181 28 178 6.17e-25 100
MS.gene071232.t1 MTR_7g008060 30.151 199 118 5 10 204 14 195 1.11e-24 99.4
MS.gene071232.t1 MTR_3g098270 36.842 171 97 5 10 174 13 178 2.09e-24 99.0
MS.gene071232.t1 MTR_8g022810 34.615 182 109 7 27 204 36 211 2.50e-24 97.8
MS.gene071232.t1 MTR_8g022810 34.615 182 109 7 27 204 36 211 3.80e-24 98.2
MS.gene071232.t1 MTR_4g035353 34.483 174 108 5 4 174 2 172 3.96e-24 98.2
MS.gene071232.t1 MTR_4g019880 29.787 188 127 3 10 196 13 196 4.28e-24 98.2
MS.gene071232.t1 MTR_0009s0250 34.911 169 106 2 8 174 9 175 9.62e-24 96.7
MS.gene071232.t1 MTR_2g017965 32.973 185 117 6 1 181 1 182 1.34e-23 96.7
MS.gene071232.t1 MTR_4g122200 38.621 145 85 3 31 174 34 175 1.40e-23 96.7
MS.gene071232.t1 MTR_3g021460 31.795 195 124 7 10 199 10 200 2.07e-23 95.9
MS.gene071232.t1 MTR_0565s0010 29.762 168 113 4 14 179 14 178 8.62e-23 94.4
MS.gene071232.t1 MTR_8g079050 33.889 180 113 4 1 174 4 183 1.20e-22 94.0
MS.gene071232.t1 MTR_3g052480 33.333 210 122 8 7 201 10 216 1.55e-22 93.6
MS.gene071232.t1 MTR_3g024510 31.285 179 104 7 29 197 35 204 2.40e-22 93.2
MS.gene071232.t1 MTR_8g074580 31.868 182 116 3 1 174 11 192 2.47e-22 93.6
MS.gene071232.t1 MTR_5g093090 32.240 183 107 6 30 200 26 203 3.81e-22 90.1
MS.gene071232.t1 MTR_2g461580 30.412 194 128 5 10 199 10 200 6.07e-22 92.0
MS.gene071232.t1 MTR_3g023220 36.486 148 88 5 29 174 30 173 8.07e-22 91.7
MS.gene071232.t1 MTR_3g005660 33.889 180 105 6 29 197 33 209 9.07e-22 91.7
MS.gene071232.t1 MTR_3g052490 34.054 185 93 7 7 174 9 181 1.32e-21 91.3
MS.gene071232.t1 MTR_5g093090 32.370 173 104 5 30 190 26 197 2.37e-21 89.7
MS.gene071232.t1 MTR_5g093090 32.240 183 107 6 30 200 26 203 5.83e-21 89.4
MS.gene071232.t1 MTR_3g435540 33.146 178 103 6 34 201 25 196 5.93e-21 89.4
MS.gene071232.t1 MTR_2g033920 33.333 168 102 5 9 174 16 175 1.06e-20 88.2
MS.gene071232.t1 MTR_2g033920 33.533 167 103 5 9 174 16 175 1.37e-20 88.6
MS.gene071232.t1 MTR_6g034400 36.364 121 76 1 85 204 4 124 2.45e-20 83.2
MS.gene071232.t1 MTR_6g049220 42.697 89 51 0 30 118 28 116 3.23e-20 84.0
MS.gene071232.t1 MTR_3g005650 31.188 202 125 7 1 197 2 194 3.33e-20 87.0
MS.gene071232.t1 MTR_4g108690 31.395 172 112 5 34 200 29 199 2.95e-19 84.7
MS.gene071232.t1 MTR_2g018015 30.387 181 119 5 29 204 33 211 3.72e-19 83.6
MS.gene071232.t1 MTR_8g022790 34.247 146 89 4 29 172 34 174 1.03e-18 83.2
MS.gene071232.t1 MTR_3g052580 30.978 184 104 9 4 174 3 176 2.39e-18 82.4
MS.gene071232.t1 MTR_8g031390 29.064 203 127 7 8 204 9 200 2.43e-18 81.6
MS.gene071232.t1 MTR_3g052580 30.978 184 104 9 4 174 2 175 2.86e-18 82.4
MS.gene071232.t1 MTR_8g031390 29.064 203 127 7 8 204 9 200 4.02e-18 81.6
MS.gene071232.t1 MTR_8g074560 34.091 176 103 6 11 174 5 179 4.30e-18 81.6
MS.gene071232.t1 MTR_2g087292 33.333 144 94 1 8 149 7 150 1.58e-17 79.0
MS.gene071232.t1 MTR_8g031420 29.143 175 112 6 32 204 35 199 1.97e-17 79.7
MS.gene071232.t1 MTR_8g031400 27.805 205 130 8 8 204 7 201 9.02e-17 77.8
MS.gene071232.t1 MTR_8g031430 28.283 198 130 6 9 204 14 201 1.09e-16 77.4
MS.gene071232.t1 MTR_5g078210 35.948 153 93 5 25 174 18 168 1.13e-16 77.0
MS.gene071232.t1 MTR_5g078210 35.948 153 93 5 25 174 18 168 1.29e-16 77.0
MS.gene071232.t1 MTR_5g062890 54.717 53 23 1 31 82 27 79 2.37e-12 61.2
MS.gene071232.t1 MTR_3g463930 27.749 191 108 7 8 174 13 197 1.29e-11 63.2
MS.gene071232.t1 MTR_3g069780 31.013 158 94 8 32 180 30 181 3.89e-11 61.6
MS.gene071232.t1 MTR_1g060500 39.326 89 49 3 33 119 41 126 6.22e-11 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene071232.t1 AT2G40250 47.399 173 91 0 32 204 36 208 5.75e-52 171
MS.gene071232.t1 AT1G58430 42.105 209 116 3 1 204 2 210 2.41e-48 161
MS.gene071232.t1 AT2G24560 42.308 208 112 4 3 204 4 209 5.28e-48 159
MS.gene071232.t1 AT5G42170 40.462 173 101 2 31 203 49 219 7.83e-48 160
MS.gene071232.t1 AT2G24560 42.308 208 112 4 3 204 4 209 1.18e-47 160
MS.gene071232.t1 AT2G31540 40.394 203 118 2 5 204 8 210 2.35e-47 159
MS.gene071232.t1 AT1G75900 39.594 197 114 2 8 203 22 214 3.78e-47 159
MS.gene071232.t1 AT1G06990 45.714 175 94 1 31 204 36 210 6.76e-47 158
MS.gene071232.t1 AT5G45960 40.909 176 99 2 32 204 47 220 7.09e-47 158
MS.gene071232.t1 AT2G04570 39.691 194 116 1 12 204 8 201 4.32e-46 155
MS.gene071232.t1 AT2G30310 39.806 206 120 3 3 204 4 209 1.11e-45 154
MS.gene071232.t1 AT4G26790 40.107 187 108 2 18 203 18 201 2.24e-45 154
MS.gene071232.t1 AT4G26790 40.107 187 108 2 18 203 18 201 2.24e-45 154
MS.gene071232.t1 AT2G30220 42.938 177 98 2 31 204 32 208 3.43e-45 153
MS.gene071232.t1 AT2G42990 39.000 200 121 1 6 204 2 201 8.24e-45 152
MS.gene071232.t1 AT1G20132 44.966 149 82 0 30 178 59 207 2.89e-44 151
MS.gene071232.t1 AT3G43550 36.364 198 120 4 10 204 6 200 3.07e-44 149
MS.gene071232.t1 AT5G63170 42.938 177 97 3 31 204 27 202 3.50e-44 150
MS.gene071232.t1 AT1G75890 42.938 177 99 2 30 204 45 221 1.21e-43 149
MS.gene071232.t1 AT3G43570 36.041 197 122 4 10 204 6 200 1.26e-43 148
MS.gene071232.t1 AT1G75890 42.938 177 99 2 30 204 74 250 2.33e-43 149
MS.gene071232.t1 AT1G20120 42.683 164 88 1 21 178 61 224 2.48e-43 149
MS.gene071232.t1 AT1G59406 37.056 197 120 3 10 204 6 200 3.37e-43 148
MS.gene071232.t1 AT1G59030 37.056 197 120 3 10 204 6 200 3.37e-43 148
MS.gene071232.t1 AT1G58725 37.056 197 120 3 10 204 6 200 3.37e-43 148
MS.gene071232.t1 AT1G75880 38.071 197 118 2 12 204 29 225 2.28e-42 146
MS.gene071232.t1 AT1G20135 40.722 194 113 2 12 203 9 202 1.14e-41 143
MS.gene071232.t1 AT1G75890 40.526 190 98 3 30 204 45 234 9.03e-41 142
MS.gene071232.t1 AT1G75880 37.879 198 118 3 12 204 29 226 1.53e-40 142
MS.gene071232.t1 AT3G14820 39.080 174 104 2 31 204 31 202 2.80e-40 140
MS.gene071232.t1 AT1G58520 36.782 174 108 2 31 204 29 200 3.15e-40 140
MS.gene071232.t1 AT1G58525 36.782 174 108 2 31 204 29 200 3.15e-40 140
MS.gene071232.t1 AT3G16370 38.251 183 96 2 31 204 29 203 2.90e-38 135
MS.gene071232.t1 AT1G75920 43.357 143 81 0 31 173 28 170 3.92e-37 132
MS.gene071232.t1 AT1G73610 44.000 150 83 1 32 180 35 184 5.05e-36 129
MS.gene071232.t1 AT1G20130 38.272 162 89 2 28 178 179 340 3.03e-35 130
MS.gene071232.t1 AT1G20130 38.272 162 89 2 28 178 199 360 3.17e-35 130
MS.gene071232.t1 AT1G23500 40.909 176 97 3 32 204 35 206 5.03e-35 126
MS.gene071232.t1 AT5G03810 35.784 204 119 4 9 204 4 203 5.54e-34 124
MS.gene071232.t1 AT1G75920 39.241 158 81 1 31 173 23 180 3.26e-33 122
MS.gene071232.t1 AT5G45950 38.043 184 110 3 14 195 22 203 5.73e-33 121
MS.gene071232.t1 AT5G22810 35.754 179 111 2 30 204 32 210 1.56e-31 117
MS.gene071232.t1 AT4G28780 36.257 171 101 4 8 174 7 173 2.87e-31 117
MS.gene071232.t1 AT3G04290 36.842 152 94 2 29 179 26 176 7.36e-31 116
MS.gene071232.t1 AT4G10950 38.095 147 89 2 30 175 69 214 8.94e-31 116
MS.gene071232.t1 AT1G75910 40.667 150 89 0 31 180 27 176 1.20e-30 115
MS.gene071232.t1 AT5G03820 35.754 179 106 2 31 204 32 206 1.47e-30 115
MS.gene071232.t1 AT5G03820 35.754 179 106 2 31 204 60 234 2.24e-30 115
MS.gene071232.t1 AT5G08460 34.682 173 108 3 31 199 48 219 9.03e-30 113
MS.gene071232.t1 AT3G53100 32.778 180 112 2 30 204 27 202 1.80e-29 112
MS.gene071232.t1 AT1G75930 42.169 166 93 3 15 177 11 176 8.49e-29 110
MS.gene071232.t1 AT5G41890 37.805 164 95 3 14 174 17 176 8.34e-28 108
MS.gene071232.t1 AT5G33370 33.735 166 108 2 32 196 30 194 1.09e-27 107
MS.gene071232.t1 AT4G10950 36.735 147 87 2 30 175 69 210 1.22e-27 108
MS.gene071232.t1 AT4G16230 36.842 152 93 2 24 174 22 171 1.99e-27 104
MS.gene071232.t1 AT5G33370 33.735 166 108 2 32 196 30 194 2.07e-27 107
MS.gene071232.t1 AT1G29660 33.548 155 97 4 29 181 29 179 5.59e-26 103
MS.gene071232.t1 AT1G75920 40.678 118 70 0 56 173 25 142 6.82e-26 102
MS.gene071232.t1 AT5G18430 31.176 170 114 2 11 179 9 176 7.12e-26 102
MS.gene071232.t1 AT1G53920 34.272 213 126 9 1 204 10 217 3.06e-25 101
MS.gene071232.t1 AT2G23540 36.782 174 102 4 9 174 21 194 7.24e-24 97.8
MS.gene071232.t1 AT2G03980 32.353 170 111 4 31 197 42 210 3.02e-23 95.9
MS.gene071232.t1 AT2G03980 32.353 170 111 4 31 197 42 210 3.02e-23 95.9
MS.gene071232.t1 AT2G03980 32.353 170 111 4 31 197 42 210 3.02e-23 95.9
MS.gene071232.t1 AT2G03980 32.353 170 111 4 31 197 42 210 3.02e-23 95.9
MS.gene071232.t1 AT3G50400 38.776 147 86 4 32 174 34 180 3.32e-23 95.9
MS.gene071232.t1 AT1G29670 31.373 153 99 4 29 179 29 177 3.62e-23 95.5
MS.gene071232.t1 AT1G29670 31.373 153 99 4 29 179 97 245 5.68e-23 95.5
MS.gene071232.t1 AT2G19060 29.808 208 131 8 1 204 1 197 2.88e-22 92.8
MS.gene071232.t1 AT5G15720 34.132 167 104 5 31 194 29 192 3.31e-22 92.0
MS.gene071232.t1 AT5G40990 32.500 200 122 8 8 199 10 204 4.97e-22 92.4
MS.gene071232.t1 AT1G53940 34.104 173 102 6 32 198 39 205 5.43e-22 91.3
MS.gene071232.t1 AT5G15720 34.132 167 104 5 31 194 29 192 5.64e-22 92.0
MS.gene071232.t1 AT1G53940 34.104 173 102 6 32 198 39 205 1.40e-21 91.3
MS.gene071232.t1 AT2G19010 31.902 163 101 4 29 191 27 179 2.45e-21 90.1
MS.gene071232.t1 AT2G19010 31.902 163 101 4 29 191 57 209 4.12e-21 90.1
MS.gene071232.t1 AT1G75920 33.566 143 64 1 31 173 28 139 5.36e-21 89.0
MS.gene071232.t1 AT1G74460 30.814 172 113 5 34 200 25 195 7.51e-21 89.4
MS.gene071232.t1 AT2G04020 32.584 178 107 6 31 204 42 210 9.31e-21 87.4
MS.gene071232.t1 AT1G71250 32.418 182 118 4 1 179 8 187 1.41e-20 88.6
MS.gene071232.t1 AT5G45670 29.310 174 118 4 8 179 5 175 1.65e-20 88.2
MS.gene071232.t1 AT2G04020 32.584 178 107 6 31 204 42 210 1.99e-20 87.4
MS.gene071232.t1 AT1G33811 34.194 155 94 5 29 179 31 181 2.64e-20 87.8
MS.gene071232.t1 AT4G18970 31.579 152 99 4 30 179 26 174 5.09e-20 86.7
MS.gene071232.t1 AT3G14225 29.545 176 118 5 32 204 37 209 6.51e-20 86.7
MS.gene071232.t1 AT4G18970 31.579 152 99 4 30 179 26 174 6.60e-20 87.0
MS.gene071232.t1 AT3G14225 29.834 181 115 6 32 204 37 213 1.60e-19 85.5
MS.gene071232.t1 AT1G53990 31.658 199 116 9 9 199 9 195 5.52e-19 84.0
MS.gene071232.t1 AT1G71691 35.329 167 101 6 30 194 53 214 7.31e-19 83.2
MS.gene071232.t1 AT1G71691 35.329 167 101 6 30 194 53 214 1.33e-18 83.2
MS.gene071232.t1 AT5G37690 33.333 168 108 4 10 174 4 170 3.61e-18 81.6
MS.gene071232.t1 AT2G19050 27.222 180 118 6 29 204 28 198 7.79e-18 79.7
MS.gene071232.t1 AT2G19050 27.222 180 118 6 29 204 28 198 1.00e-17 80.5
MS.gene071232.t1 AT4G30140 29.677 155 101 3 29 183 30 176 1.92e-17 79.7
MS.gene071232.t1 AT4G16220 30.058 173 77 3 24 161 22 185 4.77e-17 77.0
MS.gene071232.t1 AT5G55050 32.450 151 94 4 31 174 38 187 5.79e-17 78.6
MS.gene071232.t1 AT1G71120 33.523 176 95 7 30 196 29 191 1.30e-15 74.3
MS.gene071232.t1 AT5G37690 31.548 168 103 5 10 174 4 162 5.11e-14 69.7
MS.gene071232.t1 AT5G03610 30.120 166 98 7 33 197 44 192 4.79e-12 64.3
MS.gene071232.t1 AT3G09930 29.221 154 93 7 33 184 40 179 8.89e-12 63.5
MS.gene071232.t1 AT3G09930 29.221 154 93 7 33 184 40 179 8.89e-12 63.5
MS.gene071232.t1 AT1G28570 30.000 190 115 9 4 182 7 189 1.24e-11 63.2
MS.gene071232.t1 AT1G54010 28.713 202 117 8 1 195 1 182 2.28e-11 62.4
MS.gene071232.t1 AT1G28580 29.189 185 111 10 9 181 15 191 4.14e-11 61.6

Find 56 sgRNAs with CRISPR-Local

Find 70 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAACAAAATTCTGAAGATTT+TGG 0.137422 5.1:-65299458 None:intergenic
CTTAGCTAAGGTCTTGGATT+TGG 0.218569 5.1:+65299245 MS.gene071232:CDS
TAGCCAAAAGCTAGGAATTA+AGG 0.243178 5.1:+65299119 MS.gene071232:CDS
AATCCTTAATTCCTAGCTTT+TGG 0.252890 5.1:-65299122 None:intergenic
TAATCGCAAAGAACGCATTT+TGG 0.271306 5.1:-65299347 None:intergenic
TTAGCTAAGGTCTTGGATTT+GGG 0.291860 5.1:+65299246 MS.gene071232:CDS
GAGGAATGAAGAGGCTGTTT+TGG 0.301529 5.1:-65298878 None:intergenic
TGAATCTACCGGTTGGGATT+TGG 0.311553 5.1:-65299068 None:intergenic
GTCTATGTGGTTGTTGTTGC+CGG 0.316265 5.1:-65298998 None:intergenic
ACCGATAATGATTTGTTAAC+CGG 0.318424 5.1:+65299174 MS.gene071232:CDS
TTGTTAACCGGGGTTAGTTT+TGG 0.337441 5.1:+65299186 MS.gene071232:CDS
CTACCGGTTGGGATTTGGTT+TGG 0.343428 5.1:-65299063 None:intergenic
TTGAAGGCAATGGCTGAGTT+TGG 0.349295 5.1:-65298931 None:intergenic
CCCGGTTAACAAATCATTAT+CGG 0.388677 5.1:-65299175 None:intergenic
GTTGTTGTTGCCGGCATCAA+TGG 0.402175 5.1:-65298989 None:intergenic
GGCTGAGTTTGGCATTGAAA+TGG 0.422931 5.1:-65298920 None:intergenic
CGTTCTTTGCGATTAGCATT+GGG 0.423854 5.1:+65299355 MS.gene071232:CDS
TCGCCGAAGGCAAAGATGGC+AGG 0.432688 5.1:-65298961 None:intergenic
AGTGGCCTTAGCTAAGGTCT+TGG 0.434034 5.1:+65299239 MS.gene071232:CDS
CGTTTGTGAATCTACCGGTT+GGG 0.439505 5.1:-65299074 None:intergenic
ACCCCTGCCATCTTTGCCTT+CGG 0.464714 5.1:+65298958 MS.gene071232:CDS
ATAATAGAAGAGAGAGGTAG+AGG 0.471992 5.1:-65298897 None:intergenic
GCGTTCTTTGCGATTAGCAT+TGG 0.475649 5.1:+65299354 MS.gene071232:CDS
CCGTTTGTGAATCTACCGGT+TGG 0.478281 5.1:-65299075 None:intergenic
GGAGTCGCCGAAGGCAAAGA+TGG 0.493618 5.1:-65298965 None:intergenic
CCTTAGCTAAGGCCACTGTT+TGG 0.505645 5.1:-65299233 None:intergenic
CCGATAATGATTTGTTAACC+GGG 0.505924 5.1:+65299175 MS.gene071232:CDS
AATCATTATCGGTAACTTGA+GGG 0.510339 5.1:-65299164 None:intergenic
TTTCCAAACCAAATCCCAAC+CGG 0.516644 5.1:+65299060 MS.gene071232:CDS
GGCGACTCCACCATTGATGC+CGG 0.524779 5.1:+65298979 MS.gene071232:CDS
CTTTCCGTTTGTGAATCTAC+CGG 0.530447 5.1:-65299079 None:intergenic
AAATCATTATCGGTAACTTG+AGG 0.538713 5.1:-65299165 None:intergenic
CGCCGAAGGCAAAGATGGCA+GGG 0.539960 5.1:-65298960 None:intergenic
CGTTGTGACCACTTGCCCTA+CGG 0.547938 5.1:+65299030 MS.gene071232:CDS
GTTTGGAAAATCACGACCGT+AGG 0.552596 5.1:-65299046 None:intergenic
CCAAACAGTGGCCTTAGCTA+AGG 0.553598 5.1:+65299233 MS.gene071232:CDS
ATCAATGGTGGAGTCGCCGA+AGG 0.573448 5.1:-65298974 None:intergenic
TGTTTGAACAAGCTTTGTTG+AGG 0.574397 5.1:+65299283 MS.gene071232:CDS
GATTACCTTAGCCAAAAGCT+AGG 0.577099 5.1:+65299111 MS.gene071232:CDS
AGAGAGGTAGAGGAATGAAG+AGG 0.579277 5.1:-65298887 None:intergenic
ACTTGAGGGTCAAGAAAAGC+GGG 0.581751 5.1:-65299150 None:intergenic
TTTGGAAAATCACGACCGTA+GGG 0.588915 5.1:-65299045 None:intergenic
AAATGGATAATAGAAGAGAG+AGG 0.591368 5.1:-65298903 None:intergenic
CCCCGAGCCAAAACTAACCC+CGG 0.606961 5.1:-65299193 None:intergenic
GTTGTTGCCGGCATCAATGG+TGG 0.608298 5.1:-65298986 None:intergenic
AACTTGAGGGTCAAGAAAAG+CGG 0.612386 5.1:-65299151 None:intergenic
AATCACGACCGTAGGGCAAG+TGG 0.612416 5.1:-65299038 None:intergenic
GCCGAAGGCAAAGATGGCAG+GGG 0.615010 5.1:-65298959 None:intergenic
CAAATCCAAGACCTTAGCTA+AGG 0.617591 5.1:-65299244 None:intergenic
CCAACCGGTAGATTCACAAA+CGG 0.620096 5.1:+65299075 MS.gene071232:CDS
AACGCATTGCGGTGTCTATG+TGG 0.635609 5.1:-65299011 None:intergenic
CGATAATGATTTGTTAACCG+GGG 0.659598 5.1:+65299176 MS.gene071232:CDS
ATGACACAAAATACTCCACA+TGG 0.677728 5.1:+65299405 MS.gene071232:CDS
AGGTTTGGATAGCCAAACAG+TGG 0.677829 5.1:+65299221 MS.gene071232:CDS
CAAGTGGTCACAACGCATTG+CGG 0.722575 5.1:-65299022 None:intergenic
TAAGATGAAACAGAACCATG+TGG 0.723069 5.1:-65299420 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAAATTGTTGGTAATGAAA+AGG + chr5.1:65299311-65299330 MS.gene071232:CDS 20.0%
! TTGAGGATAAGAAAAATTGT+TGG + chr5.1:65299300-65299319 MS.gene071232:CDS 25.0%
!!! GAACAAAATTCTGAAGATTT+TGG - chr5.1:65299461-65299480 None:intergenic 25.0%
AAATCATTATCGGTAACTTG+AGG - chr5.1:65299168-65299187 None:intergenic 30.0%
AAATGGATAATAGAAGAGAG+AGG - chr5.1:65298906-65298925 None:intergenic 30.0%
AATCATTATCGGTAACTTGA+GGG - chr5.1:65299167-65299186 None:intergenic 30.0%
!! AATCCTTAATTCCTAGCTTT+TGG - chr5.1:65299125-65299144 None:intergenic 30.0%
!! ACCGATAATGATTTGTTAAC+CGG + chr5.1:65299174-65299193 MS.gene071232:CDS 30.0%
ATAATAGAAGAGAGAGGTAG+AGG - chr5.1:65298900-65298919 None:intergenic 35.0%
ATGACACAAAATACTCCACA+TGG + chr5.1:65299405-65299424 MS.gene071232:CDS 35.0%
CCCGGTTAACAAATCATTAT+CGG - chr5.1:65299178-65299197 None:intergenic 35.0%
TAAGATGAAACAGAACCATG+TGG - chr5.1:65299423-65299442 None:intergenic 35.0%
TAGCCAAAAGCTAGGAATTA+AGG + chr5.1:65299119-65299138 MS.gene071232:CDS 35.0%
TTAGCTAAGGTCTTGGATTT+GGG + chr5.1:65299246-65299265 MS.gene071232:CDS 35.0%
! TAATCGCAAAGAACGCATTT+TGG - chr5.1:65299350-65299369 None:intergenic 35.0%
!! CCGATAATGATTTGTTAACC+GGG + chr5.1:65299175-65299194 MS.gene071232:CDS 35.0%
!! CGATAATGATTTGTTAACCG+GGG + chr5.1:65299176-65299195 MS.gene071232:CDS 35.0%
!! TAATTCCTAGCTTTTGGCTA+AGG - chr5.1:65299119-65299138 None:intergenic 35.0%
!! TAGCTTTTGGCTAAGGTAAT+CGG - chr5.1:65299112-65299131 None:intergenic 35.0%
!! TGTTTGAACAAGCTTTGTTG+AGG + chr5.1:65299283-65299302 MS.gene071232:CDS 35.0%
AACTTGAGGGTCAAGAAAAG+CGG - chr5.1:65299154-65299173 None:intergenic 40.0%
CAAATCCAAGACCTTAGCTA+AGG - chr5.1:65299247-65299266 None:intergenic 40.0%
CGTTCTTTGCGATTAGCATT+GGG + chr5.1:65299355-65299374 MS.gene071232:CDS 40.0%
CTTAGCTAAGGTCTTGGATT+TGG + chr5.1:65299245-65299264 MS.gene071232:CDS 40.0%
CTTTCCGTTTGTGAATCTAC+CGG - chr5.1:65299082-65299101 None:intergenic 40.0%
GATTACCTTAGCCAAAAGCT+AGG + chr5.1:65299111-65299130 MS.gene071232:CDS 40.0%
TTTCCAAACCAAATCCCAAC+CGG + chr5.1:65299060-65299079 MS.gene071232:CDS 40.0%
TTTGGAAAATCACGACCGTA+GGG - chr5.1:65299048-65299067 None:intergenic 40.0%
!! TTTTGGCTAAGGTAATCGGT+TGG - chr5.1:65299108-65299127 None:intergenic 40.0%
!!! AGGGGTTTTATTGAAGGCAA+TGG - chr5.1:65298944-65298963 None:intergenic 40.0%
!!! TTGTTAACCGGGGTTAGTTT+TGG + chr5.1:65299186-65299205 MS.gene071232:CDS 40.0%
ACTTGAGGGTCAAGAAAAGC+GGG - chr5.1:65299153-65299172 None:intergenic 45.0%
AGAGAGGTAGAGGAATGAAG+AGG - chr5.1:65298890-65298909 None:intergenic 45.0%
AGGTTTGGATAGCCAAACAG+TGG + chr5.1:65299221-65299240 MS.gene071232:CDS 45.0%
CCAACCGGTAGATTCACAAA+CGG + chr5.1:65299075-65299094 MS.gene071232:CDS 45.0%
GCGTTCTTTGCGATTAGCAT+TGG + chr5.1:65299354-65299373 MS.gene071232:CDS 45.0%
GTTTGGAAAATCACGACCGT+AGG - chr5.1:65299049-65299068 None:intergenic 45.0%
TTGAAGGCAATGGCTGAGTT+TGG - chr5.1:65298934-65298953 None:intergenic 45.0%
! CGTTTGTGAATCTACCGGTT+GGG - chr5.1:65299077-65299096 None:intergenic 45.0%
! GAGGAATGAAGAGGCTGTTT+TGG - chr5.1:65298881-65298900 None:intergenic 45.0%
! GGCTGAGTTTGGCATTGAAA+TGG - chr5.1:65298923-65298942 None:intergenic 45.0%
! GTCTATGTGGTTGTTGTTGC+CGG - chr5.1:65299001-65299020 None:intergenic 45.0%
! TGAATCTACCGGTTGGGATT+TGG - chr5.1:65299071-65299090 None:intergenic 45.0%
!!! GATGGCAGGGGTTTTATTGA+AGG - chr5.1:65298950-65298969 None:intergenic 45.0%
AACGCATTGCGGTGTCTATG+TGG - chr5.1:65299014-65299033 None:intergenic 50.0%
CAAGTGGTCACAACGCATTG+CGG - chr5.1:65299025-65299044 None:intergenic 50.0%
CCGTTTGTGAATCTACCGGT+TGG - chr5.1:65299078-65299097 None:intergenic 50.0%
CCTTAGCTAAGGCCACTGTT+TGG - chr5.1:65299236-65299255 None:intergenic 50.0%
! AGTGGCCTTAGCTAAGGTCT+TGG + chr5.1:65299239-65299258 MS.gene071232:CDS 50.0%
! CCAAACAGTGGCCTTAGCTA+AGG + chr5.1:65299233-65299252 MS.gene071232:CDS 50.0%
! CTACCGGTTGGGATTTGGTT+TGG - chr5.1:65299066-65299085 None:intergenic 50.0%
! GTTGTTGTTGCCGGCATCAA+TGG - chr5.1:65298992-65299011 None:intergenic 50.0%
!!! AACCGGGGTTAGTTTTGGCT+CGG + chr5.1:65299191-65299210 MS.gene071232:CDS 50.0%
AATCACGACCGTAGGGCAAG+TGG - chr5.1:65299041-65299060 None:intergenic 55.0%
ACCCCTGCCATCTTTGCCTT+CGG + chr5.1:65298958-65298977 MS.gene071232:CDS 55.0%
CGTTGTGACCACTTGCCCTA+CGG + chr5.1:65299030-65299049 MS.gene071232:CDS 55.0%
GTTGTTGCCGGCATCAATGG+TGG - chr5.1:65298989-65299008 None:intergenic 55.0%
! ATCAATGGTGGAGTCGCCGA+AGG - chr5.1:65298977-65298996 None:intergenic 55.0%
!!! ACCGGGGTTAGTTTTGGCTC+GGG + chr5.1:65299192-65299211 MS.gene071232:CDS 55.0%
CCCCGAGCCAAAACTAACCC+CGG - chr5.1:65299196-65299215 None:intergenic 60.0%
CGCCGAAGGCAAAGATGGCA+GGG - chr5.1:65298963-65298982 None:intergenic 60.0%
GCCGAAGGCAAAGATGGCAG+GGG - chr5.1:65298962-65298981 None:intergenic 60.0%
GGAGTCGCCGAAGGCAAAGA+TGG - chr5.1:65298968-65298987 None:intergenic 60.0%
GGCGACTCCACCATTGATGC+CGG + chr5.1:65298979-65298998 MS.gene071232:CDS 60.0%
TCGCCGAAGGCAAAGATGGC+AGG - chr5.1:65298964-65298983 None:intergenic 60.0%
!!! AGTTTTGGCTCGGGGGGTTC+AGG + chr5.1:65299201-65299220 MS.gene071232:CDS 60.0%
!!! CCGGGGTTAGTTTTGGCTCG+GGG + chr5.1:65299193-65299212 MS.gene071232:CDS 60.0%
!!! CGGGGTTAGTTTTGGCTCGG+GGG + chr5.1:65299194-65299213 MS.gene071232:CDS 60.0%
!!! GGGGTTAGTTTTGGCTCGGG+GGG + chr5.1:65299195-65299214 MS.gene071232:CDS 60.0%
! TGGCTCGGGGGGTTCAGGTT+TGG + chr5.1:65299206-65299225 MS.gene071232:CDS 65.0%


Chromosome Type Strat End Strand Name
chr5.1 gene 65298871 65299482 65298871 ID=MS.gene071232
chr5.1 mRNA 65298871 65299482 65298871 ID=MS.gene071232.t1;Parent=MS.gene071232
chr5.1 exon 65298871 65299482 65298871 ID=MS.gene071232.t1.exon1;Parent=MS.gene071232.t1
chr5.1 CDS 65298871 65299482 65298871 ID=cds.MS.gene071232.t1;Parent=MS.gene071232.t1
Gene Sequence

>MS.gene071232

ATGGCATCCAAAACAGCCTCTTCATTCCTCTACCTCTCTCTTCTATTATCCATTTCAATGCCAAACTCAGCCATTGCCTTCAATAAAACCCCTGCCATCTTTGCCTTCGGCGACTCCACCATTGATGCCGGCAACAACAACCACATAGACACCGCAATGCGTTGTGACCACTTGCCCTACGGTCGTGATTTTCCAAACCAAATCCCAACCGGTAGATTCACAAACGGAAAGTTACCAACCGATTACCTTAGCCAAAAGCTAGGAATTAAGGATTTATTGCCCGCTTTTCTTGACCCTCAAGTTACCGATAATGATTTGTTAACCGGGGTTAGTTTTGGCTCGGGGGGTTCAGGTTTGGATAGCCAAACAGTGGCCTTAGCTAAGGTCTTGGATTTGGGTACACAATTTCAGTTGTTTGAACAAGCTTTGTTGAGGATAAGAAAAATTGTTGGTAATGAAAAGGCTAAAGATATAATCCAAAATGCGTTCTTTGCGATTAGCATTGGGACTAATGATATGTTGTACAGTGTTTATATGACACAAAATACTCCACATGGTTCTGTTTCATCTTATCAAGATTTTATGCTCCAAAATCTTCAGAATTTTGTTCAG
Protein sequence

>MS.gene071232.t1

MASKTASSFLYLSLLLSISMPNSAIAFNKTPAIFAFGDSTIDAGNNNHIDTAMRCDHLPYGRDFPNQIPTGRFTNGKLPTDYLSQKLGIKDLLPAFLDPQVTDNDLLTGVSFGSGGSGLDSQTVALAKVLDLGTQFQLFEQALLRIRKIVGNEKAKDIIQNAFFAISIGTNDMLYSVYMTQNTPHGSVSSYQDFMLQNLQNFVQ