Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071419.t1 | KEH35970.1 | 96.6 | 89 | 3 | 0 | 1 | 89 | 492 | 580 | 2.70E-40 | 174.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071419.t1 | Q9LYE3 | 50.6 | 89 | 44 | 0 | 1 | 89 | 472 | 560 | 1.3e-22 | 107.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071419.t1 | A0A072V2S5 | 96.6 | 89 | 3 | 0 | 1 | 89 | 395 | 483 | 1.9e-40 | 174.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049159 | MS.gene071419 | 0.817822 | 2.70E-52 | -1.69E-46 |
| MS.gene057869 | MS.gene071419 | 0.801515 | 8.68E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071419.t1 | MTR_3g109470 | 93.617 | 94 | 6 | 0 | 1 | 94 | 395 | 488 | 2.29e-56 | 183 |
| MS.gene071419.t1 | MTR_3g109470 | 93.617 | 94 | 6 | 0 | 1 | 94 | 492 | 585 | 2.97e-55 | 181 |
| MS.gene071419.t1 | MTR_3g109470 | 93.617 | 94 | 6 | 0 | 1 | 94 | 492 | 585 | 3.39e-55 | 181 |
| MS.gene071419.t1 | MTR_2g062470 | 44.944 | 89 | 49 | 0 | 1 | 89 | 371 | 459 | 1.57e-21 | 88.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071419.t1 | AT5G11470 | 50.562 | 89 | 44 | 0 | 1 | 89 | 472 | 560 | 2.45e-29 | 110 |
| MS.gene071419.t1 | AT5G11470 | 50.562 | 89 | 44 | 0 | 1 | 89 | 472 | 560 | 2.73e-29 | 110 |
| MS.gene071419.t1 | AT3G15605 | 43.158 | 95 | 54 | 0 | 1 | 95 | 217 | 311 | 3.07e-16 | 72.8 |
| MS.gene071419.t1 | AT3G15605 | 43.158 | 95 | 54 | 0 | 1 | 95 | 217 | 311 | 3.07e-16 | 72.8 |
| MS.gene071419.t1 | AT3G15605 | 43.158 | 95 | 54 | 0 | 1 | 95 | 301 | 395 | 3.50e-16 | 72.8 |
| MS.gene071419.t1 | AT3G15605 | 42.268 | 97 | 54 | 1 | 1 | 95 | 217 | 313 | 1.85e-14 | 67.8 |
Find 22 sgRNAs with CRISPR-Local
Find 70 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACTTGTGCTGCTTGAAAATT+TGG | 0.078848 | 3.3:+89925481 | MS.gene071419:CDS |
| TAAATCACAGGATATTATTT+TGG | 0.169466 | 3.3:+89925951 | MS.gene071419:intron |
| GACTAGAATATGCGGTCTTC+TGG | 0.293058 | 3.3:-89926008 | None:intergenic |
| AGAATCTGTCATTAGAAAAT+TGG | 0.317702 | 3.3:+89926187 | MS.gene071419:CDS |
| GGCTGTTTCTTGATGTCAAA+TGG | 0.319595 | 3.3:+89926215 | MS.gene071419:CDS |
| GCATATTCTAGTCCTCATTC+TGG | 0.349043 | 3.3:+89926018 | MS.gene071419:CDS |
| GCATATACAAGAAGAGGTAG+AGG | 0.423121 | 3.3:+89926424 | MS.gene071419:CDS |
| TTTAACTTCATCAGAAGTAC+AGG | 0.454276 | 3.3:+89925511 | MS.gene071419:CDS |
| TCTTTCATTCTTTCCTCCCA+AGG | 0.462766 | 3.3:-89925440 | None:intergenic |
| GCTGTTTCTTGATGTCAAAT+GGG | 0.525144 | 3.3:+89926216 | MS.gene071419:CDS |
| ATGAAAGATGCTTATGAGCA+AGG | 0.529715 | 3.3:+89925455 | MS.gene071419:CDS |
| ATCTGTCATTAGAAAATTGG+AGG | 0.543865 | 3.3:+89926190 | MS.gene071419:CDS |
| GGGAGAGCATATACAAGAAG+AGG | 0.551063 | 3.3:+89926418 | MS.gene071419:CDS |
| ACTTCTGATGAAGTTAAAGA+AGG | 0.553964 | 3.3:-89925506 | None:intergenic |
| TGAAAGATGCTTATGAGCAA+GGG | 0.557385 | 3.3:+89925456 | MS.gene071419:CDS |
| TGTCATTAGAAAATTGGAGG+AGG | 0.572214 | 3.3:+89926193 | MS.gene071419:CDS |
| TGCAGTGTCTAACCAGAATG+AGG | 0.598170 | 3.3:-89926030 | None:intergenic |
| GTCATTAGAAAATTGGAGGA+GGG | 0.609832 | 3.3:+89926194 | MS.gene071419:CDS |
| GGAGTCGATATCATAAAGTG+TGG | 0.615678 | 3.3:+89926397 | MS.gene071419:CDS |
| GTTTCTTGATGTCAAATGGG+CGG | 0.643178 | 3.3:+89926219 | MS.gene071419:CDS |
| GAGTCGATATCATAAAGTGT+GGG | 0.651952 | 3.3:+89926398 | MS.gene071419:CDS |
| AGAATGAGGACTAGAATATG+CGG | 0.713953 | 3.3:-89926016 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TCAATATTTATTTATGATAC+AGG | + | chr3.3:89926128-89926147 | MS.gene071419:intron | 15.0% |
| !!! | TTTTGCTATATTTAAAAGAA+AGG | + | chr3.3:89926157-89926176 | MS.gene071419:CDS | 15.0% |
| !!! | ATTTGTACCATTTATTTTTG+GGG | + | chr3.3:89925757-89925776 | MS.gene071419:intron | 20.0% |
| !!! | CATTTGTACCATTTATTTTT+GGG | + | chr3.3:89925756-89925775 | MS.gene071419:intron | 20.0% |
| !!! | TAAATCACAGGATATTATTT+TGG | + | chr3.3:89925951-89925970 | MS.gene071419:intron | 20.0% |
| !!! | TGAGAGATTTTATTTAGATT+CGG | + | chr3.3:89926369-89926388 | MS.gene071419:intron | 20.0% |
| ! | AGAATCTGTCATTAGAAAAT+TGG | + | chr3.3:89926187-89926206 | MS.gene071419:CDS | 25.0% |
| ! | TTAATCACCCCAAAAATAAA+TGG | - | chr3.3:89925767-89925786 | None:intergenic | 25.0% |
| ! | TTAGTAAGATAACAACAAAG+TGG | - | chr3.3:89925897-89925916 | None:intergenic | 25.0% |
| ! | TTTATTTGATAGACTCTTGT+AGG | + | chr3.3:89925303-89925322 | MS.gene071419:intron | 25.0% |
| !!! | AGAGATTTTATTTAGATTCG+GGG | + | chr3.3:89926371-89926390 | MS.gene071419:intron | 25.0% |
| !!! | CTTTGTAGTTGTGTTTTATT+TGG | + | chr3.3:89925713-89925732 | MS.gene071419:intron | 25.0% |
| !!! | GAGAGATTTTATTTAGATTC+GGG | + | chr3.3:89926370-89926389 | MS.gene071419:intron | 25.0% |
| !!! | GCATTTGTACCATTTATTTT+TGG | + | chr3.3:89925755-89925774 | MS.gene071419:intron | 25.0% |
| !!! | TGTTTTATTTGGAATAGAAG+AGG | + | chr3.3:89925724-89925743 | MS.gene071419:intron | 25.0% |
| !!! | TTTTTTATCTTTCCGGAAAT+CGG | + | chr3.3:89925346-89925365 | MS.gene071419:intron | 25.0% |
| ACTTCTGATGAAGTTAAAGA+AGG | - | chr3.3:89925509-89925528 | None:intergenic | 30.0% | |
| AGTGGATGAAATTGTCATTA+AGG | - | chr3.3:89925879-89925898 | None:intergenic | 30.0% | |
| ATCTGTCATTAGAAAATTGG+AGG | + | chr3.3:89926190-89926209 | MS.gene071419:CDS | 30.0% | |
| ATTGAAACACAGATAAGAAG+AGG | - | chr3.3:89926113-89926132 | None:intergenic | 30.0% | |
| CACAACTACAAAGAAAAACA+AGG | - | chr3.3:89925706-89925725 | None:intergenic | 30.0% | |
| CAGTTATCAAGAATAGACAA+AGG | - | chr3.3:89926253-89926272 | None:intergenic | 30.0% | |
| GGTAATAATCATTCCAAAAC+CGG | + | chr3.3:89925532-89925551 | MS.gene071419:intron | 30.0% | |
| GTGGATGAAATTGTCATTAA+GGG | - | chr3.3:89925878-89925897 | None:intergenic | 30.0% | |
| TCTAAATAAAATCTCTCACG+AGG | - | chr3.3:89926367-89926386 | None:intergenic | 30.0% | |
| TTCAGATTCTTGTAGGTTAT+GGG | + | chr3.3:89925559-89925578 | MS.gene071419:intron | 30.0% | |
| TTTCAGATTCTTGTAGGTTA+TGG | + | chr3.3:89925558-89925577 | MS.gene071419:intron | 30.0% | |
| ! | TTTAACTTCATCAGAAGTAC+AGG | + | chr3.3:89925511-89925530 | MS.gene071419:CDS | 30.0% |
| !! | ATTTTATTTAGATTCGGGGA+GGG | + | chr3.3:89926375-89926394 | MS.gene071419:intron | 30.0% |
| !! | TTTGGGGTGATTAACTTTTA+AGG | + | chr3.3:89925773-89925792 | MS.gene071419:intron | 30.0% |
| !!! | GTTGCTCTTTTTTATCTTTC+CGG | + | chr3.3:89925339-89925358 | MS.gene071419:intron | 30.0% |
| !!! | TCACAGGATATTATTTTGGA+TGG | + | chr3.3:89925955-89925974 | MS.gene071419:intron | 30.0% |
| !!! | TTTTTTACGTTTTCAGCCTT+GGG | + | chr3.3:89925424-89925443 | MS.gene071419:intron | 30.0% |
| ACACAGATAAGAAGAGGAAT+AGG | - | chr3.3:89926107-89926126 | None:intergenic | 35.0% | |
| ACGTCAAAGATATTAGAGAC+AGG | - | chr3.3:89925924-89925943 | None:intergenic | 35.0% | |
| AGAATGAGGACTAGAATATG+CGG | - | chr3.3:89926019-89926038 | None:intergenic | 35.0% | |
| ATGAAAGATGCTTATGAGCA+AGG | + | chr3.3:89925455-89925474 | MS.gene071419:CDS | 35.0% | |
| CAAAGATATTAGAGACAGGA+AGG | - | chr3.3:89925920-89925939 | None:intergenic | 35.0% | |
| CTACAAGAATCTGAAAATGC+CGG | - | chr3.3:89925554-89925573 | None:intergenic | 35.0% | |
| GAGTCGATATCATAAAGTGT+GGG | + | chr3.3:89926398-89926417 | MS.gene071419:CDS | 35.0% | |
| GTCATTAGAAAATTGGAGGA+GGG | + | chr3.3:89926194-89926213 | MS.gene071419:CDS | 35.0% | |
| TCAGATTCTTGTAGGTTATG+GGG | + | chr3.3:89925560-89925579 | MS.gene071419:intron | 35.0% | |
| TGAAAGATGCTTATGAGCAA+GGG | + | chr3.3:89925456-89925475 | MS.gene071419:CDS | 35.0% | |
| TGTCATTAGAAAATTGGAGG+AGG | + | chr3.3:89926193-89926212 | MS.gene071419:CDS | 35.0% | |
| ! | GACGTACTTTTCTAAATCAC+AGG | + | chr3.3:89925939-89925958 | MS.gene071419:intron | 35.0% |
| ! | TTGATAACTGCACTAGCAAA+TGG | + | chr3.3:89926263-89926282 | MS.gene071419:intron | 35.0% |
| !! | ACTCTAGAGTGGTTAATTTG+TGG | + | chr3.3:89925654-89925673 | MS.gene071419:intron | 35.0% |
| !! | ACTTGTGCTGCTTGAAAATT+TGG | + | chr3.3:89925481-89925500 | MS.gene071419:CDS | 35.0% |
| !! | CTCTAGAGTGGTTAATTTGT+GGG | + | chr3.3:89925655-89925674 | MS.gene071419:intron | 35.0% |
| !! | GATTTTATTTAGATTCGGGG+AGG | + | chr3.3:89926374-89926393 | MS.gene071419:intron | 35.0% |
| !! | GCTGTTTCTTGATGTCAAAT+GGG | + | chr3.3:89926216-89926235 | MS.gene071419:CDS | 35.0% |
| !! | TCTAGAGTGGTTAATTTGTG+GGG | + | chr3.3:89925656-89925675 | MS.gene071419:intron | 35.0% |
| !! | TTTTATTTAGATTCGGGGAG+GGG | + | chr3.3:89926376-89926395 | MS.gene071419:intron | 35.0% |
| !!! | CTTTTTTACGTTTTCAGCCT+TGG | + | chr3.3:89925423-89925442 | MS.gene071419:intron | 35.0% |
| CAATTGCATTGTCCGATTTC+CGG | - | chr3.3:89925361-89925380 | None:intergenic | 40.0% | |
| CTACATAGGATTCAACATGC+TGG | - | chr3.3:89925634-89925653 | None:intergenic | 40.0% | |
| GCATATTCTAGTCCTCATTC+TGG | + | chr3.3:89926018-89926037 | MS.gene071419:CDS | 40.0% | |
| GGAGTCGATATCATAAAGTG+TGG | + | chr3.3:89926397-89926416 | MS.gene071419:CDS | 40.0% | |
| GTTTCTTGATGTCAAATGGG+CGG | + | chr3.3:89926219-89926238 | MS.gene071419:CDS | 40.0% | |
| TCTTTCATTCTTTCCTCCCA+AGG | - | chr3.3:89925443-89925462 | None:intergenic | 40.0% | |
| ! | ATCCTATGTAGACTCTAGAG+TGG | + | chr3.3:89925643-89925662 | MS.gene071419:intron | 40.0% |
| ! | CGGCATTTTCAGATTCTTGT+AGG | + | chr3.3:89925552-89925571 | MS.gene071419:intron | 40.0% |
| !! | AACCACTCTAGAGTCTACAT+AGG | - | chr3.3:89925648-89925667 | None:intergenic | 40.0% |
| !! | ACAAGGAGTACTGATAAACG+AGG | - | chr3.3:89925689-89925708 | None:intergenic | 40.0% |
| !! | GGCTGTTTCTTGATGTCAAA+TGG | + | chr3.3:89926215-89926234 | MS.gene071419:CDS | 40.0% |
| !!! | GAATCTGAAAATGCCGGTTT+TGG | - | chr3.3:89925548-89925567 | None:intergenic | 40.0% |
| GACTAGAATATGCGGTCTTC+TGG | - | chr3.3:89926011-89926030 | None:intergenic | 45.0% | |
| TGCAGTGTCTAACCAGAATG+AGG | - | chr3.3:89926033-89926052 | None:intergenic | 45.0% | |
| ! | GGGAGAGCATATACAAGAAG+AGG | + | chr3.3:89926418-89926437 | MS.gene071419:CDS | 45.0% |
| ! | TTTACGTTTTCAGCCTTGGG+AGG | + | chr3.3:89925427-89925446 | MS.gene071419:intron | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 89925264 | 89926443 | 89925264 | ID=MS.gene071419 |
| chr3.3 | mRNA | 89925264 | 89926443 | 89925264 | ID=MS.gene071419.t1;Parent=MS.gene071419 |
| chr3.3 | exon | 89925264 | 89925266 | 89925264 | ID=MS.gene071419.t1.exon1;Parent=MS.gene071419.t1 |
| chr3.3 | CDS | 89925264 | 89925266 | 89925264 | ID=cds.MS.gene071419.t1;Parent=MS.gene071419.t1 |
| chr3.3 | exon | 89925440 | 89925532 | 89925440 | ID=MS.gene071419.t1.exon2;Parent=MS.gene071419.t1 |
| chr3.3 | CDS | 89925440 | 89925532 | 89925440 | ID=cds.MS.gene071419.t1;Parent=MS.gene071419.t1 |
| chr3.3 | exon | 89925961 | 89926039 | 89925961 | ID=MS.gene071419.t1.exon3;Parent=MS.gene071419.t1 |
| chr3.3 | CDS | 89925961 | 89926039 | 89925961 | ID=cds.MS.gene071419.t1;Parent=MS.gene071419.t1 |
| chr3.3 | exon | 89926150 | 89926240 | 89926150 | ID=MS.gene071419.t1.exon4;Parent=MS.gene071419.t1 |
| chr3.3 | CDS | 89926150 | 89926240 | 89926150 | ID=cds.MS.gene071419.t1;Parent=MS.gene071419.t1 |
| chr3.3 | exon | 89926386 | 89926443 | 89926386 | ID=MS.gene071419.t1.exon5;Parent=MS.gene071419.t1 |
| chr3.3 | CDS | 89926386 | 89926443 | 89926386 | ID=cds.MS.gene071419.t1;Parent=MS.gene071419.t1 |
| Gene Sequence |
| Protein sequence |
