Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071642.t1 | RHN80248.1 | 97.1 | 309 | 9 | 0 | 1 | 309 | 1 | 309 | 1.40E-137 | 499.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071642.t1 | D2XNQ8 | 90.0 | 309 | 31 | 0 | 1 | 309 | 1 | 309 | 9.3e-135 | 481.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071642.t1 | A0A396JVK3 | 97.1 | 309 | 9 | 0 | 1 | 309 | 1 | 309 | 9.9e-138 | 499.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene055317 | MS.gene071642 | 0.990204 | 1.54E-181 | -1.69E-46 |
| MS.gene055318 | MS.gene071642 | 0.930894 | 8.41E-94 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071642.t1 | MTR_1g073400 | 97.735 | 309 | 7 | 0 | 1 | 309 | 1 | 309 | 0.0 | 616 |
| MS.gene071642.t1 | MTR_3g106480 | 92.880 | 309 | 22 | 0 | 1 | 309 | 1 | 309 | 0.0 | 588 |
| MS.gene071642.t1 | MTR_3g106485 | 92.880 | 309 | 22 | 0 | 1 | 309 | 1 | 309 | 0.0 | 565 |
| MS.gene071642.t1 | MTR_1g115145 | 93.548 | 310 | 16 | 2 | 1 | 309 | 1 | 307 | 0.0 | 564 |
| MS.gene071642.t1 | MTR_3g106420 | 91.909 | 309 | 25 | 0 | 1 | 309 | 3 | 311 | 0.0 | 557 |
| MS.gene071642.t1 | MTR_1g071130 | 88.997 | 309 | 8 | 1 | 1 | 309 | 1 | 283 | 0.0 | 556 |
| MS.gene071642.t1 | MTR_1g083190 | 89.968 | 309 | 31 | 0 | 1 | 309 | 3 | 311 | 0.0 | 541 |
| MS.gene071642.t1 | MTR_3g106430 | 92.557 | 309 | 23 | 0 | 1 | 309 | 1 | 309 | 0.0 | 537 |
| MS.gene071642.t1 | MTR_3g106470 | 75.405 | 309 | 20 | 2 | 1 | 309 | 32 | 284 | 1.05e-148 | 425 |
| MS.gene071642.t1 | MTR_8g063830 | 78.105 | 306 | 33 | 3 | 1 | 305 | 1 | 273 | 3.38e-142 | 408 |
| MS.gene071642.t1 | MTR_3g065540 | 66.087 | 115 | 12 | 1 | 40 | 154 | 14 | 101 | 4.30e-43 | 144 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071642.t1 | AT5G25250 | 82.524 | 309 | 54 | 0 | 1 | 309 | 1 | 309 | 0.0 | 534 |
| MS.gene071642.t1 | AT5G25260 | 81.553 | 309 | 57 | 0 | 1 | 309 | 1 | 309 | 0.0 | 526 |
| MS.gene071642.t1 | AT5G64870 | 79.545 | 308 | 63 | 0 | 2 | 309 | 3 | 310 | 8.44e-170 | 480 |
Find 0 sgRNAs with CRISPR-Local
Find 81 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATGAATATTTCTCATATTTA+GGG | - | 11330:7952-7971 | MS.gene071642:CDS | 15.0% |
| !! | CATGAATATTTCTCATATTT+AGG | - | 11330:7951-7970 | MS.gene071642:CDS | 20.0% |
| AAATCAGTTTAGCGTATTTG+AGG | + | 11330:7713-7732 | MS.gene071641:intergenic | 30.0% | |
| CAAACTTGCAAAGAAATCAT+GGG | - | 11330:7542-7561 | MS.gene071642:CDS | 30.0% | |
| TATGAGAAATATTCATGACC+AGG | + | 11330:7948-7967 | MS.gene071642:intergenic | 30.0% | |
| TCAAACTTGCAAAGAAATCA+TGG | - | 11330:7541-7560 | MS.gene071642:CDS | 30.0% | |
| TGTAAATCAATAACCCGAAT+TGG | + | 11330:7896-7915 | MS.gene071642:intergenic | 30.0% | |
| AACTCGAACTAAACCAATTC+GGG | - | 11330:7880-7899 | MS.gene071642:CDS | 35.0% | |
| CAACTCGAACTAAACCAATT+CGG | - | 11330:7879-7898 | MS.gene071642:CDS | 35.0% | |
| CAATGCGAATGTTAAGCAAT+TGG | - | 11330:7914-7933 | MS.gene071642:CDS | 35.0% | |
| GAAAGTTATTGCTATGCAGA+GGG | - | 11330:8106-8125 | MS.gene071642:CDS | 35.0% | |
| TAAACTCTCGAATCATGTCA+AGG | - | 11330:7743-7762 | MS.gene071642:CDS | 35.0% | |
| TAGTTCAAGGAATCATCGAA+GGG | - | 11330:7769-7788 | MS.gene071642:CDS | 35.0% | |
| TGATTCGAGAGTTTATCATG+AGG | + | 11330:7738-7757 | MS.gene071642:intergenic | 35.0% | |
| ! | AAGAACGAAAGGCAGTTTTT+CGG | + | 11330:7643-7662 | MS.gene071642:intergenic | 35.0% |
| !! | GGTTTTTGAGAATCAAAGAG+AGG | - | 11330:8175-8194 | MS.gene071642:CDS | 35.0% |
| AGTTCAAGGAATCATCGAAG+GGG | - | 11330:7770-7789 | MS.gene071642:CDS | 40.0% | |
| CATGTCAAGGAACTAGTTCA+AGG | - | 11330:7756-7775 | MS.gene071642:CDS | 40.0% | |
| CGAAAGTTATTGCTATGCAG+AGG | - | 11330:8105-8124 | MS.gene071642:CDS | 40.0% | |
| CTAGTTCAAGGAATCATCGA+AGG | - | 11330:7768-7787 | MS.gene071642:CDS | 40.0% | |
| GAATGCAGCAAAAATTGACG+CGG | - | 11330:8079-8098 | MS.gene071642:CDS | 40.0% | |
| GCTAAGATGAAAGGTGAAGT+TGG | - | 11330:8026-8045 | MS.gene071642:CDS | 40.0% | |
| TAGAGTTAGGACTGAAGTGA+AGG | - | 11330:8154-8173 | MS.gene071642:CDS | 40.0% | |
| TCAGTTTAGCGTATTTGAGG+AGG | + | 11330:7710-7729 | MS.gene071642:intergenic | 40.0% | |
| TGCGAATGTTAAGCAATTGG+TGG | - | 11330:7917-7936 | MS.gene071642:CDS | 40.0% | |
| ! | AAGAAATCATGGGTTTTCCC+TGG | - | 11330:7552-7571 | MS.gene071642:CDS | 40.0% |
| ! | ACAAGGAGGGATTTAGAGTT+AGG | - | 11330:8141-8160 | MS.gene071642:CDS | 40.0% |
| ! | ACTTCGAAGGTATAGTTCAC+CGG | + | 11330:7606-7625 | MS.gene071642:intergenic | 40.0% |
| ! | TTTAGGGCAAAAGACGCAAA+TGG | - | 11330:7968-7987 | MS.gene071642:CDS | 40.0% |
| !! | TGAAGTTGGCTCGAAATCTA+GGG | - | 11330:8040-8059 | MS.gene071642:CDS | 40.0% |
| ACTCATGGCTTGAACTTCGA+AGG | + | 11330:7619-7638 | MS.gene071642:intergenic | 45.0% | |
| AGGACCAATTGTGAAGACAG+CGG | + | 11330:7667-7686 | MS.gene071642:intergenic | 45.0% | |
| GAATCAAAGAGAGGCTGAAG+TGG | - | 11330:8184-8203 | MS.gene071642:CDS | 45.0% | |
| GTGGCAGAAGCTAAGATGAA+AGG | - | 11330:8017-8036 | MS.gene071642:CDS | 45.0% | |
| ! | ACCATGGAAGAGGTTTTCAG+AGG | - | 11330:7819-7838 | MS.gene071642:CDS | 45.0% |
| ! | TCCTCTGAAAACCTCTTCCA+TGG | + | 11330:7823-7842 | MS.gene071642:intergenic | 45.0% |
| ! | TGAGTTGGCGAAGAAGAAAG+CGG | - | 11330:8220-8239 | MS.gene071642:CDS | 45.0% |
| !! | GTGAAGTTGGCTCGAAATCT+AGG | - | 11330:8039-8058 | MS.gene071642:CDS | 45.0% |
| !! | TGCTAAAGCTGTGGCTCTAA+GGG | - | 11330:8280-8299 | MS.gene071642:CDS | 45.0% |
| AAGACAGCGGGAAGAACGAA+AGG | + | 11330:7654-7673 | MS.gene071642:intergenic | 50.0% | |
| AAGACGGTGCAAGATTGACC+AGG | + | 11330:7573-7592 | MS.gene071642:intergenic | 50.0% | |
| AGACGGTGCAAGATTGACCA+GGG | + | 11330:7572-7591 | MS.gene071642:intergenic | 50.0% | |
| AGGTATAGTTCACCGGTGAG+AGG | + | 11330:7599-7618 | MS.gene071642:intergenic | 50.0% | |
| AGTTGCAAGGTGAGGTTGAG+AGG | - | 11330:8309-8328 | MS.gene071642:CDS | 50.0% | |
| CTCAACCTCACCTTGCAACT+CGG | + | 11330:8309-8328 | MS.gene071642:intergenic | 50.0% | |
| CTTCCCGCTGTCTTCACAAT+TGG | - | 11330:7660-7679 | MS.gene071642:CDS | 50.0% | |
| CTTTCCTGATCGTCAACACG+AGG | + | 11330:7687-7706 | MS.gene071642:intergenic | 50.0% | |
| GAATCAAGCTAGGGTTGACG+TGG | - | 11330:7998-8017 | MS.gene071642:CDS | 50.0% | |
| GATGTCTTCGATTCCAGCTC+CGG | + | 11330:7523-7542 | MS.gene071642:intergenic | 50.0% | |
| GGAAGCAGCGAATCAAGCTA+GGG | - | 11330:7989-8008 | MS.gene071642:CDS | 50.0% | |
| GGACCAATTGTGAAGACAGC+GGG | + | 11330:7666-7685 | MS.gene071642:intergenic | 50.0% | |
| GGCTGAGGCTAATTCTGAGT+TGG | - | 11330:8205-8224 | MS.gene071642:CDS | 50.0% | |
| GTTATTGCTATGCAGAGGGC+TGG | - | 11330:8110-8129 | MS.gene071642:CDS | 50.0% | |
| TCCGAGTACCTGGTGATAAC+CGG | - | 11330:7501-7520 | MS.gene071642:CDS | 50.0% | |
| TCCGGTTATCACCAGGTACT+CGG | + | 11330:7505-7524 | MS.gene071642:intergenic | 50.0% | |
| TGCTTCGATGACCATGGAAG+AGG | - | 11330:7809-7828 | MS.gene071642:CDS | 50.0% | |
| TGGAAGCAGCGAATCAAGCT+AGG | - | 11330:7988-8007 | MS.gene071642:CDS | 50.0% | |
| ! | AAGCAATTGGTGGATGTGCC+TGG | - | 11330:7927-7946 | MS.gene071642:CDS | 50.0% |
| ! | CTGCTAAAGCTGTGGCTCTA+AGG | - | 11330:8279-8298 | MS.gene071642:CDS | 50.0% |
| ! | GAAGAAAGCGGCTTGGACTA+AGG | - | 11330:8232-8251 | MS.gene071642:CDS | 50.0% |
| !! | AGCAAAAGCATCCGAGTACC+TGG | - | 11330:7491-7510 | MS.gene071642:CDS | 50.0% |
| !! | AGGCAGTTTTTCGGCACTCA+TGG | + | 11330:7634-7653 | MS.gene071642:intergenic | 50.0% |
| !! | GTTGGCTCGAAATCTAGGGA+AGG | - | 11330:8044-8063 | MS.gene071642:CDS | 50.0% |
| AAGAGAGGCTGAAGTGGCTG+AGG | - | 11330:8190-8209 | MS.gene071642:CDS | 55.0% | |
| AGTCGAGGCTGCTAAAGCTG+TGG | - | 11330:8271-8290 | MS.gene071642:CDS | 55.0% | |
| CTAAGGGAAGCCGAGTTGCA+AGG | - | 11330:8296-8315 | MS.gene071642:CDS | 55.0% | |
| GCTGGAGAGAGTGACAAGGA+GGG | - | 11330:8128-8147 | MS.gene071642:CDS | 55.0% | |
| TACCTGGTGATAACCGGAGC+TGG | - | 11330:7507-7526 | MS.gene071642:CDS | 55.0% | |
| TGGCGAAGAAGAAAGCGGCT+TGG | - | 11330:8225-8244 | MS.gene071642:CDS | 55.0% | |
| TTCCAGCTCCGGTTATCACC+AGG | + | 11330:7512-7531 | MS.gene071642:intergenic | 55.0% | |
| TTGGACTAAGGCGGCTCAAG+TGG | - | 11330:8244-8263 | MS.gene071642:CDS | 55.0% | |
| ! | GAAAGCGGCTTGGACTAAGG+CGG | - | 11330:8235-8254 | MS.gene071642:CDS | 55.0% |
| ! | TGGTCCTCGTGTTGACGATC+AGG | - | 11330:7680-7699 | MS.gene071642:CDS | 55.0% |
| CACCGGTGAGAGGTCGAAGA+CGG | + | 11330:7589-7608 | MS.gene071642:intergenic | 60.0% | |
| CACCGTCTTCGACCTCTCAC+CGG | - | 11330:7584-7603 | MS.gene071642:CDS | 60.0% | |
| CCATGGTCATCGAAGCAGCG+AGG | + | 11330:7806-7825 | MS.gene071642:intergenic | 60.0% | |
| CCTCGCTGCTTCGATGACCA+TGG | - | 11330:7803-7822 | MS.gene071642:CDS | 60.0% | |
| GAGGGCTGGAGAGAGTGACA+AGG | - | 11330:8124-8143 | MS.gene071642:CDS | 60.0% | |
| GGAAGCCGAGTTGCAAGGTG+AGG | - | 11330:8301-8320 | MS.gene071642:CDS | 60.0% | |
| GGCTCAAGTGGCTGAAGTCG+AGG | - | 11330:8256-8275 | MS.gene071642:CDS | 60.0% | |
| GGCTGGAGAGAGTGACAAGG+AGG | - | 11330:8127-8146 | MS.gene071642:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 11330 | gene | 7480 | 8406 | 7480 | ID=MS.gene071642 |
| 11330 | mRNA | 7480 | 8406 | 7480 | ID=MS.gene071642.t1;Parent=MS.gene071642 |
| 11330 | exon | 7480 | 8406 | 7480 | ID=MS.gene071642.t1.exon1;Parent=MS.gene071642.t1 |
| 11330 | CDS | 7480 | 8406 | 7480 | ID=cds.MS.gene071642.t1;Parent=MS.gene071642.t1 |
| Gene Sequence |
| Protein sequence |
