Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene055317.t1 | RHN80248.1 | 96.8 | 280 | 9 | 0 | 1 | 280 | 1 | 280 | 2.00E-122 | 448.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene055317.t1 | D2XNQ8 | 89.3 | 280 | 30 | 0 | 1 | 280 | 1 | 280 | 4.6e-120 | 432.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene055317.t1 | A0A396JVK3 | 96.8 | 280 | 9 | 0 | 1 | 280 | 1 | 280 | 1.4e-122 | 448.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene055317 | MS.gene055318 | 0.923099 | 4.15E-89 | -1.69E-46 |
| MS.gene055317 | MS.gene071642 | 0.990204 | 1.54E-181 | -1.69E-46 |
| MS.gene055317 | MS.gene32118 | 0.85603 | 4.24E-62 | -1.69E-46 |
| MS.gene055317 | MS.gene40045 | 0.836547 | 8.78E-57 | -1.69E-46 |
| MS.gene055317 | MS.gene51436 | 0.822566 | 2.21E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene055317.t1 | MTR_1g073400 | 97.857 | 280 | 6 | 0 | 1 | 280 | 1 | 280 | 0.0 | 562 |
| MS.gene055317.t1 | MTR_3g106480 | 92.857 | 280 | 20 | 0 | 1 | 280 | 1 | 280 | 0.0 | 535 |
| MS.gene055317.t1 | MTR_1g115145 | 94.643 | 280 | 12 | 1 | 1 | 280 | 1 | 277 | 0.0 | 520 |
| MS.gene055317.t1 | MTR_3g106485 | 92.857 | 280 | 20 | 0 | 1 | 280 | 1 | 280 | 0.0 | 511 |
| MS.gene055317.t1 | MTR_1g071130 | 88.530 | 279 | 32 | 0 | 1 | 279 | 1 | 279 | 0.0 | 506 |
| MS.gene055317.t1 | MTR_3g106420 | 91.429 | 280 | 24 | 0 | 1 | 280 | 3 | 282 | 4.88e-179 | 502 |
| MS.gene055317.t1 | MTR_1g083190 | 90.000 | 280 | 28 | 0 | 1 | 280 | 3 | 282 | 2.08e-174 | 490 |
| MS.gene055317.t1 | MTR_3g106430 | 92.857 | 280 | 20 | 0 | 1 | 280 | 1 | 280 | 5.20e-173 | 487 |
| MS.gene055317.t1 | MTR_8g063830 | 78.214 | 280 | 28 | 2 | 1 | 280 | 1 | 247 | 1.47e-129 | 375 |
| MS.gene055317.t1 | MTR_3g106470 | 73.929 | 280 | 17 | 2 | 1 | 280 | 32 | 255 | 2.80e-129 | 375 |
| MS.gene055317.t1 | MTR_3g065540 | 66.087 | 115 | 12 | 1 | 40 | 154 | 14 | 101 | 2.82e-43 | 144 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene055317.t1 | AT5G25250 | 82.796 | 279 | 48 | 0 | 1 | 279 | 1 | 279 | 1.06e-173 | 488 |
| MS.gene055317.t1 | AT5G25260 | 81.720 | 279 | 51 | 0 | 1 | 279 | 1 | 279 | 7.65e-171 | 481 |
| MS.gene055317.t1 | AT5G64870 | 79.496 | 278 | 57 | 0 | 2 | 279 | 3 | 280 | 2.20e-152 | 434 |
Find 0 sgRNAs with CRISPR-Local
Find 92 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTAATACTTTAATATCTTT+TGG | - | 11199:44152-44171 | MS.gene055317:CDS | 10.0% |
| !! | AGTAATTAATAAATGAAAGA+AGG | + | 11199:44100-44119 | MS.gene055317:intergenic | 15.0% |
| !! | ATGAATATTTCTCATATTTA+GGG | - | 11199:43620-43639 | MS.gene055317:CDS | 15.0% |
| !! | CATTATAAAACTAAATCATA+TGG | - | 11199:44328-44347 | MS.gene055317:CDS | 15.0% |
| !!! | TGTTCTAAATTAATTTTGTT+AGG | - | 11199:44384-44403 | MS.gene055317:CDS | 15.0% |
| !! | CATGAATATTTCTCATATTT+AGG | - | 11199:43619-43638 | MS.gene055317:CDS | 20.0% |
| !! | TTAAAATGTTATATGGACAA+TGG | - | 11199:44252-44271 | MS.gene055317:CDS | 20.0% |
| !!! | AATGGAATAGTCAAATTTTT+TGG | - | 11199:44270-44289 | MS.gene055317:CDS | 20.0% |
| !!! | TCACTGTAGTTAAAGTTTTT+TGG | - | 11199:44186-44205 | MS.gene055317:CDS | 25.0% |
| AAATCAGTTTAGCGTATTTG+AGG | + | 11199:43381-43400 | MS.gene055316:intergenic | 30.0% | |
| AAGAAGGAGTTATATTTGCA+TGG | + | 11199:44084-44103 | MS.gene055317:intergenic | 30.0% | |
| CAAACTTGCAAAGAAATCAT+GGG | - | 11199:43210-43229 | MS.gene055317:intron | 30.0% | |
| TATGAGAAATATTCATGACC+AGG | + | 11199:43616-43635 | MS.gene055317:intergenic | 30.0% | |
| TCAAACTTGCAAAGAAATCA+TGG | - | 11199:43209-43228 | MS.gene055317:intron | 30.0% | |
| TGTAAATCAATAACCCGAAT+TGG | + | 11199:43564-43583 | MS.gene055317:intergenic | 30.0% | |
| AACTCGAACTAAACCAATTC+GGG | - | 11199:43548-43567 | MS.gene055317:intron | 35.0% | |
| CAACTCGAACTAAACCAATT+CGG | - | 11199:43547-43566 | MS.gene055317:intron | 35.0% | |
| CAATGCGAATGTTAAGCAAT+TGG | - | 11199:43582-43601 | MS.gene055317:intron | 35.0% | |
| GAAAGTTATTGCTATGCAGA+GGG | - | 11199:43774-43793 | MS.gene055317:CDS | 35.0% | |
| TAAACTCTCGAATCATGTCA+AGG | - | 11199:43411-43430 | MS.gene055317:intron | 35.0% | |
| TAGTTCAAGGAATCATCGAA+GGG | - | 11199:43437-43456 | MS.gene055317:intron | 35.0% | |
| TGATTCGAGAGTTTATCATG+AGG | + | 11199:43406-43425 | MS.gene055317:intergenic | 35.0% | |
| ! | AAGAACGAAAGGCAGTTTTT+CGG | + | 11199:43311-43330 | MS.gene055317:intergenic | 35.0% |
| ! | ATATTTGCATGGACTAACCT+TGG | + | 11199:44073-44092 | MS.gene055317:intergenic | 35.0% |
| ! | TAGTGTTGAGTATGAAACCA+AGG | - | 11199:44053-44072 | MS.gene055317:CDS | 35.0% |
| !! | GGTTTTTGAGAATCAAAGAG+AGG | - | 11199:43843-43862 | MS.gene055317:CDS | 35.0% |
| AGTTCAAGGAATCATCGAAG+GGG | - | 11199:43438-43457 | MS.gene055317:intron | 40.0% | |
| CATGTCAAGGAACTAGTTCA+AGG | - | 11199:43424-43443 | MS.gene055317:intron | 40.0% | |
| CGAAAGTTATTGCTATGCAG+AGG | - | 11199:43773-43792 | MS.gene055317:CDS | 40.0% | |
| CTAGTTCAAGGAATCATCGA+AGG | - | 11199:43436-43455 | MS.gene055317:intron | 40.0% | |
| GAATGCAGCAAAAATTGACG+CGG | - | 11199:43747-43766 | MS.gene055317:CDS | 40.0% | |
| GCTAAGATGAAAGGTGAAGT+TGG | - | 11199:43694-43713 | MS.gene055317:CDS | 40.0% | |
| TAGAGTTAGGACTGAAGTGA+AGG | - | 11199:43822-43841 | MS.gene055317:CDS | 40.0% | |
| TCAGTTTAGCGTATTTGAGG+AGG | + | 11199:43378-43397 | MS.gene055317:intergenic | 40.0% | |
| TGCGAATGTTAAGCAATTGG+TGG | - | 11199:43585-43604 | MS.gene055317:CDS | 40.0% | |
| ! | AAGAAATCATGGGTTTTCCC+TGG | - | 11199:43220-43239 | MS.gene055317:intron | 40.0% |
| ! | ACAAGGAGGGATTTAGAGTT+AGG | - | 11199:43809-43828 | MS.gene055317:CDS | 40.0% |
| ! | ACTTCGAAGGTATAGTTCAC+CGG | + | 11199:43274-43293 | MS.gene055317:intergenic | 40.0% |
| ! | TTTAGGGCAAAAGACGCAAA+TGG | - | 11199:43636-43655 | MS.gene055317:CDS | 40.0% |
| !! | TGAAGTTGGCTCGAAATCTA+GGG | - | 11199:43708-43727 | MS.gene055317:CDS | 40.0% |
| ACTCATGGCTTGAACTTCGA+AGG | + | 11199:43287-43306 | MS.gene055317:intergenic | 45.0% | |
| AGGACCAATTGTGAAGACAG+CGG | + | 11199:43335-43354 | MS.gene055317:intergenic | 45.0% | |
| GAATCAAAGAGAGGCTGAAG+TGG | - | 11199:43852-43871 | MS.gene055317:CDS | 45.0% | |
| GTGGCAGAAGCTAAGATGAA+AGG | - | 11199:43685-43704 | MS.gene055317:CDS | 45.0% | |
| ! | ACCATGGAAGAGGTTTTCAG+AGG | - | 11199:43487-43506 | MS.gene055317:intron | 45.0% |
| ! | TCCTCTGAAAACCTCTTCCA+TGG | + | 11199:43491-43510 | MS.gene055317:intergenic | 45.0% |
| ! | TGAGTTGGCGAAGAAGAAAG+CGG | - | 11199:43888-43907 | MS.gene055317:CDS | 45.0% |
| !! | GTGAAGTTGGCTCGAAATCT+AGG | - | 11199:43707-43726 | MS.gene055317:CDS | 45.0% |
| !! | TGCTAAAGCTGTGGCTCTAA+GGG | - | 11199:43948-43967 | MS.gene055317:CDS | 45.0% |
| !!! | TTATTTTTTAAAATGTTATA+TGG | - | 11199:44245-44264 | MS.gene055317:CDS | 5.0% |
| AAGACAGCGGGAAGAACGAA+AGG | + | 11199:43322-43341 | MS.gene055317:intergenic | 50.0% | |
| AAGACGGTGCAAGATTGACC+AGG | + | 11199:43241-43260 | MS.gene055317:intergenic | 50.0% | |
| AGACGGTGCAAGATTGACCA+GGG | + | 11199:43240-43259 | MS.gene055317:intergenic | 50.0% | |
| AGGTATAGTTCACCGGTGAG+AGG | + | 11199:43267-43286 | MS.gene055317:intergenic | 50.0% | |
| AGTTGCAAGGTGAGGTTGAG+AGG | - | 11199:43977-43996 | MS.gene055317:CDS | 50.0% | |
| CTCAACCTCACCTTGCAACT+CGG | + | 11199:43977-43996 | MS.gene055317:intergenic | 50.0% | |
| CTTCCCGCTGTCTTCACAAT+TGG | - | 11199:43328-43347 | MS.gene055317:intron | 50.0% | |
| CTTTCCTGATCGTCAACACG+AGG | + | 11199:43355-43374 | MS.gene055317:intergenic | 50.0% | |
| GAATCAAGCTAGGGTTGACG+TGG | - | 11199:43666-43685 | MS.gene055317:CDS | 50.0% | |
| GATGTCTTCGATTCCAGCTC+CGG | + | 11199:43191-43210 | MS.gene055317:intergenic | 50.0% | |
| GGAAGCAGCGAATCAAGCTA+GGG | - | 11199:43657-43676 | MS.gene055317:CDS | 50.0% | |
| GGACCAATTGTGAAGACAGC+GGG | + | 11199:43334-43353 | MS.gene055317:intergenic | 50.0% | |
| GGCTGAGGCTAATTCTGAGT+TGG | - | 11199:43873-43892 | MS.gene055317:CDS | 50.0% | |
| GTTATTGCTATGCAGAGGGC+TGG | - | 11199:43778-43797 | MS.gene055317:CDS | 50.0% | |
| TCCGAGTACCTGGTGATAAC+CGG | - | 11199:43169-43188 | MS.gene055317:intron | 50.0% | |
| TCCGGTTATCACCAGGTACT+CGG | + | 11199:43173-43192 | MS.gene055317:intergenic | 50.0% | |
| TGCTTCGATGACCATGGAAG+AGG | - | 11199:43477-43496 | MS.gene055317:intron | 50.0% | |
| TGGAAGCAGCGAATCAAGCT+AGG | - | 11199:43656-43675 | MS.gene055317:CDS | 50.0% | |
| ! | AAGCAATTGGTGGATGTGCC+TGG | - | 11199:43595-43614 | MS.gene055317:CDS | 50.0% |
| ! | CTGCTAAAGCTGTGGCTCTA+AGG | - | 11199:43947-43966 | MS.gene055317:CDS | 50.0% |
| ! | GAAGAAAGCGGCTTGGACTA+AGG | - | 11199:43900-43919 | MS.gene055317:CDS | 50.0% |
| !! | AGCAAAAGCATCCGAGTACC+TGG | - | 11199:43159-43178 | MS.gene055317:intron | 50.0% |
| !! | AGGCAGTTTTTCGGCACTCA+TGG | + | 11199:43302-43321 | MS.gene055317:intergenic | 50.0% |
| !! | GTTGGCTCGAAATCTAGGGA+AGG | - | 11199:43712-43731 | MS.gene055317:CDS | 50.0% |
| AAGAGAGGCTGAAGTGGCTG+AGG | - | 11199:43858-43877 | MS.gene055317:CDS | 55.0% | |
| AGTCGAGGCTGCTAAAGCTG+TGG | - | 11199:43939-43958 | MS.gene055317:CDS | 55.0% | |
| CTAAGGGAAGCCGAGTTGCA+AGG | - | 11199:43964-43983 | MS.gene055317:CDS | 55.0% | |
| GCTGGAGAGAGTGACAAGGA+GGG | - | 11199:43796-43815 | MS.gene055317:CDS | 55.0% | |
| TACCTGGTGATAACCGGAGC+TGG | - | 11199:43175-43194 | MS.gene055317:intron | 55.0% | |
| TGGCGAAGAAGAAAGCGGCT+TGG | - | 11199:43893-43912 | MS.gene055317:CDS | 55.0% | |
| TTCCAGCTCCGGTTATCACC+AGG | + | 11199:43180-43199 | MS.gene055317:intergenic | 55.0% | |
| TTGGACTAAGGCGGCTCAAG+TGG | - | 11199:43912-43931 | MS.gene055317:CDS | 55.0% | |
| ! | GAAAGCGGCTTGGACTAAGG+CGG | - | 11199:43903-43922 | MS.gene055317:CDS | 55.0% |
| ! | TGGTCCTCGTGTTGACGATC+AGG | - | 11199:43348-43367 | MS.gene055317:intron | 55.0% |
| CACCGGTGAGAGGTCGAAGA+CGG | + | 11199:43257-43276 | MS.gene055317:intergenic | 60.0% | |
| CACCGTCTTCGACCTCTCAC+CGG | - | 11199:43252-43271 | MS.gene055317:intron | 60.0% | |
| CCATGGTCATCGAAGCAGCG+AGG | + | 11199:43474-43493 | MS.gene055317:intergenic | 60.0% | |
| CCTCGCTGCTTCGATGACCA+TGG | - | 11199:43471-43490 | MS.gene055317:intron | 60.0% | |
| GAGGGCTGGAGAGAGTGACA+AGG | - | 11199:43792-43811 | MS.gene055317:CDS | 60.0% | |
| GGAAGCCGAGTTGCAAGGTG+AGG | - | 11199:43969-43988 | MS.gene055317:CDS | 60.0% | |
| GGCTCAAGTGGCTGAAGTCG+AGG | - | 11199:43924-43943 | MS.gene055317:CDS | 60.0% | |
| GGCTGGAGAGAGTGACAAGG+AGG | - | 11199:43795-43814 | MS.gene055317:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 11199 | gene | 43148 | 44422 | 43148 | ID=MS.gene055317 |
| 11199 | mRNA | 43148 | 44422 | 43148 | ID=MS.gene055317.t1;Parent=MS.gene055317 |
| 11199 | exon | 43585 | 44422 | 43585 | ID=MS.gene055317.t1.exon1;Parent=MS.gene055317.t1 |
| 11199 | CDS | 43585 | 44422 | 43585 | ID=cds.MS.gene055317.t1;Parent=MS.gene055317.t1 |
| 11199 | exon | 43148 | 43164 | 43148 | ID=MS.gene055317.t1.exon2;Parent=MS.gene055317.t1 |
| 11199 | CDS | 43148 | 43164 | 43148 | ID=cds.MS.gene055317.t1;Parent=MS.gene055317.t1 |
| Gene Sequence |
| Protein sequence |
