Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071710.t1 | XP_003629610.1 | 90.3 | 62 | 6 | 0 | 8 | 69 | 324 | 385 | 2.00E-24 | 121.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071710.t1 | P32112 | 83.9 | 62 | 10 | 0 | 8 | 69 | 324 | 385 | 1.3e-24 | 113.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071710.t1 | Q84RD9 | 90.3 | 62 | 6 | 0 | 8 | 69 | 324 | 385 | 1.4e-24 | 121.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052818 | MS.gene071710 | 0.814223 | 1.72E-51 | -1.69E-46 |
| MS.gene059362 | MS.gene071710 | 0.822816 | 1.93E-53 | -1.69E-46 |
| MS.gene059872 | MS.gene071710 | 0.800143 | 1.65E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071710.t1 | MTR_8g083090 | 84.848 | 66 | 10 | 0 | 5 | 70 | 321 | 386 | 2.63e-34 | 121 |
| MS.gene071710.t1 | MTR_3g084340 | 75.758 | 66 | 16 | 0 | 5 | 70 | 321 | 386 | 1.90e-31 | 114 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071710.t1 | AT4G13940 | 77.273 | 66 | 15 | 0 | 5 | 70 | 200 | 265 | 1.13e-30 | 110 |
| MS.gene071710.t1 | AT4G13940 | 77.273 | 66 | 15 | 0 | 5 | 70 | 276 | 341 | 1.17e-30 | 111 |
| MS.gene071710.t1 | AT3G23810 | 80.952 | 63 | 12 | 0 | 8 | 70 | 324 | 386 | 2.08e-30 | 111 |
| MS.gene071710.t1 | AT4G13940 | 77.273 | 66 | 15 | 0 | 5 | 70 | 321 | 386 | 3.32e-30 | 110 |
Find 28 sgRNAs with CRISPR-Local
Find 27 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AATGATGCGGTGTTGGTCTC+AGG | 0.345045 | 4.2:+13181912 | None:intergenic |
| CATGATGATGTCCTTGTTAC+CGG | 0.390795 | 4.2:+13182079 | None:intergenic |
| GACAATGAAATTGATATGTC+TGG | 0.422786 | 4.2:-13181999 | MS.gene071710:CDS |
| ACCTGTCGGTTTGAGCTTGA+TGG | 0.428961 | 4.2:+13181943 | None:intergenic |
| AATGCCATTGTTTGCAACAT+TGG | 0.434082 | 4.2:-13182029 | MS.gene071710:CDS |
| GATGTCCTTGTTACCGGTGG+TGG | 0.469485 | 4.2:+13182085 | None:intergenic |
| TGGTGAAGCGGCTTCACACC+AGG | 0.493590 | 4.2:+13181963 | None:intergenic |
| CCGCATCATTGTCTTGGCCG+AGG | 0.532043 | 4.2:-13181899 | MS.gene071710:CDS |
| CCAAGACAATGATGCGGTGT+TGG | 0.534943 | 4.2:+13181905 | None:intergenic |
| CGCATCATTGTCTTGGCCGA+GGG | 0.536223 | 4.2:-13181898 | MS.gene071710:CDS |
| ACCATCAAGCTCAAACCGAC+AGG | 0.545928 | 4.2:-13181944 | MS.gene071710:CDS |
| CCAACACCGCATCATTGTCT+TGG | 0.552216 | 4.2:-13181905 | MS.gene071710:CDS |
| ACATCTTTGTTACCCACCAC+CGG | 0.557088 | 4.2:-13182098 | MS.gene071710:CDS |
| GTTACCCACCACCGGTAACA+AGG | 0.565596 | 4.2:-13182090 | MS.gene071710:CDS |
| ATGATGCGGTGTTGGTCTCA+GGG | 0.574678 | 4.2:+13181913 | None:intergenic |
| TCATCATGGTTGACGACATG+AGG | 0.586533 | 4.2:-13182064 | MS.gene071710:CDS |
| GTTTGAGCTTGATGGTGAAG+CGG | 0.588729 | 4.2:+13181951 | None:intergenic |
| GTGACCAATGTTGCAAACAA+TGG | 0.589198 | 4.2:+13182025 | None:intergenic |
| AAGTTCTATCAAACGACCCT+CGG | 0.590721 | 4.2:+13181882 | None:intergenic |
| CGGTAACAAGGACATCATCA+TGG | 0.591464 | 4.2:-13182078 | MS.gene071710:CDS |
| ATCAAGCTCAAACCGACAGG+TGG | 0.591767 | 4.2:-13181941 | MS.gene071710:CDS |
| ATGTCCTTGTTACCGGTGGT+GGG | 0.614050 | 4.2:+13182086 | None:intergenic |
| CTCAGGGAAAACCCACCTGT+CGG | 0.631363 | 4.2:+13181929 | None:intergenic |
| TCAAGCTCAAACCGACAGGT+GGG | 0.660823 | 4.2:-13181940 | MS.gene071710:CDS |
| TCTGGACTTGAGAACTACCC+TGG | 0.662161 | 4.2:-13181981 | MS.gene071710:CDS |
| GGTGAAGCGGCTTCACACCA+GGG | 0.662983 | 4.2:+13181964 | None:intergenic |
| GATGATGTCCTTGTTACCGG+TGG | 0.708198 | 4.2:+13182082 | None:intergenic |
| CCTCGGCCAAGACAATGATG+CGG | 0.733973 | 4.2:+13181899 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| GACAATGAAATTGATATGTC+TGG | - | chr4.2:13181995-13182014 | MS.gene071710:CDS | 30.0% | |
| AATGCCATTGTTTGCAACAT+TGG | - | chr4.2:13181965-13181984 | MS.gene071710:CDS | 35.0% | |
| GTGACCAATGTTGCAAACAA+TGG | + | chr4.2:13181972-13181991 | None:intergenic | 40.0% | |
| ! | CATGATGATGTCCTTGTTAC+CGG | + | chr4.2:13181918-13181937 | None:intergenic | 40.0% |
| ACATCTTTGTTACCCACCAC+CGG | - | chr4.2:13181896-13181915 | MS.gene071710:CDS | 45.0% | |
| CGGTAACAAGGACATCATCA+TGG | - | chr4.2:13181916-13181935 | MS.gene071710:CDS | 45.0% | |
| TCATCATGGTTGACGACATG+AGG | - | chr4.2:13181930-13181949 | MS.gene071710:CDS | 45.0% | |
| !! | GTTTGAGCTTGATGGTGAAG+CGG | + | chr4.2:13182046-13182065 | None:intergenic | 45.0% |
| ACCATCAAGCTCAAACCGAC+AGG | - | chr4.2:13182050-13182069 | MS.gene071710:CDS | 50.0% | |
| ATCAAGCTCAAACCGACAGG+TGG | - | chr4.2:13182053-13182072 | MS.gene071710:CDS | 50.0% | |
| ATGTCCTTGTTACCGGTGGT+GGG | + | chr4.2:13181911-13181930 | None:intergenic | 50.0% | |
| CCAACACCGCATCATTGTCT+TGG | - | chr4.2:13182089-13182108 | MS.gene071710:CDS | 50.0% | |
| CCAAGACAATGATGCGGTGT+TGG | + | chr4.2:13182092-13182111 | None:intergenic | 50.0% | |
| GATGATGTCCTTGTTACCGG+TGG | + | chr4.2:13181915-13181934 | None:intergenic | 50.0% | |
| TCAAGCTCAAACCGACAGGT+GGG | - | chr4.2:13182054-13182073 | MS.gene071710:CDS | 50.0% | |
| TCTGGACTTGAGAACTACCC+TGG | - | chr4.2:13182013-13182032 | MS.gene071710:CDS | 50.0% | |
| !! | AATGATGCGGTGTTGGTCTC+AGG | + | chr4.2:13182085-13182104 | None:intergenic | 50.0% |
| !! | ACCTGTCGGTTTGAGCTTGA+TGG | + | chr4.2:13182054-13182073 | None:intergenic | 50.0% |
| !! | ATGATGCGGTGTTGGTCTCA+GGG | + | chr4.2:13182084-13182103 | None:intergenic | 50.0% |
| CCTCGGCCAAGACAATGATG+CGG | + | chr4.2:13182098-13182117 | None:intergenic | 55.0% | |
| CGCATCATTGTCTTGGCCGA+GGG | - | chr4.2:13182096-13182115 | MS.gene071710:CDS | 55.0% | |
| CTCAGGGAAAACCCACCTGT+CGG | + | chr4.2:13182068-13182087 | None:intergenic | 55.0% | |
| GATGTCCTTGTTACCGGTGG+TGG | + | chr4.2:13181912-13181931 | None:intergenic | 55.0% | |
| GTTACCCACCACCGGTAACA+AGG | - | chr4.2:13181904-13181923 | MS.gene071710:CDS | 55.0% | |
| CCGCATCATTGTCTTGGCCG+AGG | - | chr4.2:13182095-13182114 | MS.gene071710:CDS | 60.0% | |
| GGTGAAGCGGCTTCACACCA+GGG | + | chr4.2:13182033-13182052 | None:intergenic | 60.0% | |
| TGGTGAAGCGGCTTCACACC+AGG | + | chr4.2:13182034-13182053 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.2 | gene | 13181890 | 13182126 | 13181890 | ID=MS.gene071710 |
| chr4.2 | mRNA | 13181890 | 13182126 | 13181890 | ID=MS.gene071710.t1;Parent=MS.gene071710 |
| chr4.2 | exon | 13181890 | 13182126 | 13181890 | ID=MS.gene071710.t1.exon1;Parent=MS.gene071710.t1 |
| chr4.2 | CDS | 13181890 | 13182126 | 13181890 | ID=cds.MS.gene071710.t1;Parent=MS.gene071710.t1 |
| Gene Sequence |
| Protein sequence |
