AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene059872


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene059872.t1 MTR_2g080050 99.010 101 1 0 1 101 959 1059 3.60e-65 212
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene059872.t1 AT5G54440 69.307 101 30 1 1 101 960 1059 1.14e-44 154
MS.gene059872.t1 AT5G54440 69.307 101 30 1 1 101 960 1059 1.22e-44 154
MS.gene059872.t1 AT5G54440 69.307 101 30 1 1 101 960 1059 1.26e-44 154

Find 24 sgRNAs with CRISPR-Local

Find 88 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAAGATGGATTTGTTCATTC+TGG 0.189962 2.3:-17728386 MS.gene059872:CDS
ATAGCTCTAAAACTTGAATT+TGG 0.208032 2.3:-17729646 MS.gene059872:CDS
AGTGGGAAGAAGCTTGATTT+AGG 0.339290 2.3:+17728347 None:intergenic
ACTAAACAAAATTCCTGCTT+TGG 0.355602 2.3:+17728307 None:intergenic
CAAAATTCCTGCTTTGGAAG+TGG 0.363715 2.3:+17728313 None:intergenic
ATCAAGCCAAGCATCGTATA+TGG 0.435464 2.3:+17728412 None:intergenic
GAAGTGGGAGATATGATTAG+TGG 0.465095 2.3:+17728329 None:intergenic
CTATGTGAGGACACGTGTTA+CGG 0.468702 2.3:-17729422 MS.gene059872:CDS
GCTTGGCTTGATCTTCAAGA+TGG 0.485212 2.3:-17728401 MS.gene059872:CDS
ATATCTCCCACTTCCAAAGC+AGG 0.489370 2.3:-17728320 MS.gene059872:CDS
AATACTTCACTCTGAAGTAA+AGG 0.499484 2.3:-17728444 MS.gene059872:CDS
AAAATTCCTGCTTTGGAAGT+GGG 0.503747 2.3:+17728314 None:intergenic
TGATGGTACACTGCTTCTGC+AGG 0.519077 2.3:-17729389 MS.gene059872:intron
TGTTGACCATATACGATGCT+TGG 0.525977 2.3:-17728418 MS.gene059872:CDS
GTTACGGATAAATGCAATGA+TGG 0.530071 2.3:-17729406 MS.gene059872:CDS
ACACGTGTCCTCACATAGAA+AGG 0.532823 2.3:+17729427 None:intergenic
GATGTGTAAAATGCACTGCT+AGG 0.541628 2.3:+17729449 None:intergenic
ACCTTTCAAATATCTGATTG+TGG 0.553853 2.3:+17729617 None:intergenic
AAGTGGGAGATATGATTAGT+GGG 0.557690 2.3:+17728330 None:intergenic
TCCACAATCAGATATTTGAA+AGG 0.558857 2.3:-17729618 MS.gene059872:intron
TGTTCATTCTGGACAAACTG+AGG 0.580236 2.3:-17728375 MS.gene059872:CDS
ATGTGTAAAATGCACTGCTA+GGG 0.584379 2.3:+17729450 None:intergenic
TTACACATCCTTTCTATGTG+AGG 0.593309 2.3:-17729435 MS.gene059872:CDS
GTTCATTCTGGACAAACTGA+GGG 0.611224 2.3:-17728374 MS.gene059872:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTAAACAATTATGAAAATA+AGG + chr2.3:17728608-17728627 None:intergenic 10.0%
!! AGGAAAAAAAAAATTATAGA+TGG + chr2.3:17728685-17728704 None:intergenic 15.0%
!!! AAATATAAAAATGATGGAAA+TGG + chr2.3:17728380-17728399 None:intergenic 15.0%
!! CAAAATATACAAAGAAATGT+AGG - chr2.3:17729247-17729266 MS.gene059872:intron 20.0%
!!! CTACATTTCTTTGTATATTT+TGG + chr2.3:17729249-17729268 None:intergenic 20.0%
!!! GTTTGATGATTTTTATATAG+AGG - chr2.3:17729179-17729198 MS.gene059872:intron 20.0%
!!! TATTTGAACAACCATTTTAT+GGG - chr2.3:17729207-17729226 MS.gene059872:intron 20.0%
!!! TTATTTGAACAACCATTTTA+TGG - chr2.3:17729206-17729225 MS.gene059872:intron 20.0%
! AAAAATGTCATAAATCTCAG+TGG + chr2.3:17729439-17729458 None:intergenic 25.0%
! AAAAATTATAGATGGTTCCT+AGG + chr2.3:17728677-17728696 None:intergenic 25.0%
! CTCAAAATATTAAGCTGTTT+GGG + chr2.3:17728896-17728915 None:intergenic 25.0%
! TCATAAAAGTTGTCACAAAA+TGG - chr2.3:17729340-17729359 MS.gene059872:intron 25.0%
! TCTCAAAATATTAAGCTGTT+TGG + chr2.3:17728897-17728916 None:intergenic 25.0%
! TTCCTAGGCATATATATAAT+GGG + chr2.3:17728662-17728681 None:intergenic 25.0%
! TTCCTTCATATGTAAAAAGT+TGG + chr2.3:17728748-17728767 None:intergenic 25.0%
!! ATTTTAGATATGTCAGTCAT+AGG - chr2.3:17728828-17728847 MS.gene059872:intron 25.0%
!!! AATATTTTACCTTGTTATCC+AGG - chr2.3:17728490-17728509 MS.gene059872:intron 25.0%
!!! ATAGCTCTAAAACTTGAATT+TGG - chr2.3:17728318-17728337 MS.gene059872:CDS 25.0%
!!! GATTTTTAGTATATTGTTGC+CGG + chr2.3:17729133-17729152 None:intergenic 25.0%
!!! GCTTAATATTTTGAGATTGA+TGG - chr2.3:17728901-17728920 MS.gene059872:intron 25.0%
AAAGTTCATTTGGAAGAATG+GGG + chr2.3:17728987-17729006 None:intergenic 30.0%
AACAATCGAACATATTCAAC+CGG - chr2.3:17729111-17729130 MS.gene059872:intron 30.0%
AATGAACTTTCAACATAAGG+TGG - chr2.3:17728997-17729016 MS.gene059872:intron 30.0%
ACCTTTCAAATATCTGATTG+TGG + chr2.3:17728350-17728369 None:intergenic 30.0%
ATATTAAGCTGTTTGGGTAA+AGG + chr2.3:17728890-17728909 None:intergenic 30.0%
ATTGCTTTCTGTGCTAAAAT+AGG - chr2.3:17728708-17728727 MS.gene059872:intron 30.0%
CCAAATGAACTTTCAACATA+AGG - chr2.3:17728994-17729013 MS.gene059872:intron 30.0%
CCTTATGTTGAAAGTTCATT+TGG + chr2.3:17728997-17729016 None:intergenic 30.0%
GAAAGTTCATTTGGAAGAAT+GGG + chr2.3:17728988-17729007 None:intergenic 30.0%
GTTCCTAGGCATATATATAA+TGG + chr2.3:17728663-17728682 None:intergenic 30.0%
TCATAAGTTGTCCCATAAAA+TGG + chr2.3:17729221-17729240 None:intergenic 30.0%
TCCACAATCAGATATTTGAA+AGG - chr2.3:17728346-17728365 MS.gene059872:CDS 30.0%
TGAAAGTTCATTTGGAAGAA+TGG + chr2.3:17728989-17729008 None:intergenic 30.0%
TTCTGTGCTAAAATAGGAAA+TGG - chr2.3:17728714-17728733 MS.gene059872:intron 30.0%
! ATTTTCTCTCTCTATTGCTT+TGG + chr2.3:17729287-17729306 None:intergenic 30.0%
! CACCAACTTTTTACATATGA+AGG - chr2.3:17728743-17728762 MS.gene059872:intron 30.0%
!! AATACTTCACTCTGAAGTAA+AGG - chr2.3:17729520-17729539 MS.gene059872:intron 30.0%
!! CTGTTTCTTTTTCTGCAATT+AGG - chr2.3:17728459-17728478 MS.gene059872:intron 30.0%
!!! TATTTTAGCACAGAAAGCAA+TGG + chr2.3:17728709-17728728 None:intergenic 30.0%
AAGTGGGAGATATGATTAGT+GGG + chr2.3:17729637-17729656 None:intergenic 35.0%
AGGAACTGATTACTCTTTGA+TGG - chr2.3:17728763-17728782 MS.gene059872:intron 35.0%
AGTACACAAAAACCATGTGT+AGG + chr2.3:17728441-17728460 None:intergenic 35.0%
ATGTGTAAAATGCACTGCTA+GGG + chr2.3:17728517-17728536 None:intergenic 35.0%
CACCCATTATATATATGCCT+AGG - chr2.3:17728657-17728676 MS.gene059872:intron 35.0%
GGAACTGATTACTCTTTGAT+GGG - chr2.3:17728764-17728783 MS.gene059872:intron 35.0%
GTACACAAAAACCATGTGTA+GGG + chr2.3:17728440-17728459 None:intergenic 35.0%
GTTACGGATAAATGCAATGA+TGG - chr2.3:17728558-17728577 MS.gene059872:intron 35.0%
TTACACATCCTTTCTATGTG+AGG - chr2.3:17728529-17728548 MS.gene059872:intron 35.0%
! CAAGATGGATTTGTTCATTC+TGG - chr2.3:17729578-17729597 MS.gene059872:intron 35.0%
! CTAGGCATATATATAATGGG+TGG + chr2.3:17728659-17728678 None:intergenic 35.0%
! GATTGATGGTTTATGATGTG+TGG - chr2.3:17728915-17728934 MS.gene059872:intron 35.0%
!! AAAATTCCTGCTTTGGAAGT+GGG + chr2.3:17729653-17729672 None:intergenic 35.0%
AGTGGGAAGAAGCTTGATTT+AGG + chr2.3:17729620-17729639 None:intergenic 40.0%
GAAGTGGGAGATATGATTAG+TGG + chr2.3:17729638-17729657 None:intergenic 40.0%
GATGTGTAAAATGCACTGCT+AGG + chr2.3:17728518-17728537 None:intergenic 40.0%
GTTCATTCTGGACAAACTGA+GGG - chr2.3:17729590-17729609 MS.gene059872:intron 40.0%
TACAAGTCACATGAACCAGT+GGG + chr2.3:17729497-17729516 None:intergenic 40.0%
TCCAAACCACATGGTTATAG+AGG + chr2.3:17728949-17728968 None:intergenic 40.0%
TGTTCATTCTGGACAAACTG+AGG - chr2.3:17729589-17729608 MS.gene059872:intron 40.0%
TGTTGACCATATACGATGCT+TGG - chr2.3:17729546-17729565 MS.gene059872:intron 40.0%
TTAGCACAGAAAGCAATGGA+AGG + chr2.3:17728705-17728724 None:intergenic 40.0%
! ATAACCATGTGGTTTGGAGT+TGG - chr2.3:17728951-17728970 MS.gene059872:intron 40.0%
! ATCAAGCCAAGCATCGTATA+TGG + chr2.3:17729555-17729574 None:intergenic 40.0%
! TAACCATGTGGTTTGGAGTT+GGG - chr2.3:17728952-17728971 MS.gene059872:intron 40.0%
!! CAAAATTCCTGCTTTGGAAG+TGG + chr2.3:17729654-17729673 None:intergenic 40.0%
ACACGTGTCCTCACATAGAA+AGG + chr2.3:17728540-17728559 None:intergenic 45.0%
ATATCTCCCACTTCCAAAGC+AGG - chr2.3:17729644-17729663 MS.gene059872:CDS 45.0%
CTACAAGTCACATGAACCAG+TGG + chr2.3:17729498-17729517 None:intergenic 45.0%
CTATGTGAGGACACGTGTTA+CGG - chr2.3:17728542-17728561 MS.gene059872:intron 45.0%
GCCTCTATAACCATGTGGTT+TGG - chr2.3:17728945-17728964 MS.gene059872:intron 45.0%
TCGAAGCCTCTATAACCATG+TGG - chr2.3:17728940-17728959 MS.gene059872:intron 45.0%
! CTGTTGAAGTACCCTACACA+TGG - chr2.3:17728426-17728445 MS.gene059872:CDS 45.0%
! GCTTGGCTTGATCTTCAAGA+TGG - chr2.3:17729563-17729582 MS.gene059872:intron 45.0%
!! CACTGGTTCATGTGACTTGT+AGG - chr2.3:17729496-17729515 MS.gene059872:intron 45.0%
!! TTTGGTTGATAAGCACCCAC+TGG - chr2.3:17729479-17729498 MS.gene059872:intron 45.0%
!! AAATTAAAATATAAAAATGA+TGG + chr2.3:17728386-17728405 None:intergenic 5.0%
!!! TGAATATTTTTTATATTATT+TGG - chr2.3:17729461-17729480 MS.gene059872:intron 5.0%
AAAATGCACTGCTAGGGTCC+TGG + chr2.3:17728511-17728530 None:intergenic 50.0%
CGCAAAGGCCTCTTGTATGA+GGG - chr2.3:17729043-17729062 MS.gene059872:intron 50.0%
GAACCCAACTCCAAACCACA+TGG + chr2.3:17728958-17728977 None:intergenic 50.0%
GCAAAGGCCTCTTGTATGAG+GGG - chr2.3:17729044-17729063 MS.gene059872:intron 50.0%
GTACACTGCTTCTGCAGGTA+TGG - chr2.3:17728580-17728599 MS.gene059872:intron 50.0%
TGACATGCCCCTCATACAAG+AGG + chr2.3:17729054-17729073 None:intergenic 50.0%
TGATGGTACACTGCTTCTGC+AGG - chr2.3:17728575-17728594 MS.gene059872:intron 50.0%
TGCTAGGGTCCTGGATAACA+AGG + chr2.3:17728502-17728521 None:intergenic 50.0%
TGTCCATGCTTCAAGCGCAA+AGG - chr2.3:17729028-17729047 MS.gene059872:intron 50.0%
AGGCCTTTGCGCTTGAAGCA+TGG + chr2.3:17729034-17729053 None:intergenic 55.0%
GCGCAAAGGCCTCTTGTATG+AGG - chr2.3:17729042-17729061 MS.gene059872:intron 55.0%


Chromosome Type Strat End Strand Name
chr2.3 gene 17728309 17729677 17728309 ID=MS.gene059872
chr2.3 mRNA 17728309 17729677 17728309 ID=MS.gene059872.t1;Parent=MS.gene059872
chr2.3 exon 17729619 17729677 17729619 ID=MS.gene059872.t1.exon1;Parent=MS.gene059872.t1
chr2.3 CDS 17729619 17729677 17729619 ID=cds.MS.gene059872.t1;Parent=MS.gene059872.t1
chr2.3 exon 17729390 17729474 17729390 ID=MS.gene059872.t1.exon2;Parent=MS.gene059872.t1
chr2.3 CDS 17729390 17729474 17729390 ID=cds.MS.gene059872.t1;Parent=MS.gene059872.t1
chr2.3 exon 17728309 17728468 17728309 ID=MS.gene059872.t1.exon3;Parent=MS.gene059872.t1
chr2.3 CDS 17728309 17728468 17728309 ID=cds.MS.gene059872.t1;Parent=MS.gene059872.t1
Gene Sequence

>MS.gene059872

ATGAGAACAATAGCTCTAAAACTTGAATTTGGAGCATTCCACAATCAGATATTTGAAAGGTTTTTTTTCCATTTCCATCATTTTTATATTTTAATTTTAATCATCATATAGATACATCTGTTGAAGTACCCTACACATGGTTTTTGTGTACTGTTTCTTTTTCTGCAATTAGGTACATAATAATATTTTACCTTGTTATCCAGGACCCTAGCAGTGCATTTTACACATCCTTTCTATGTGAGGACACGTGTTACGGATAAATGCAATGATGGTACACTGCTTCTGCAGGTATGGATCCTTATTTTCATAATTGTTTAATTAACACTTGATTATATGAAAAAAATAGACCACCCATTATATATATGCCTAGGAACCATCTATAATTTTTTTTTTCCTTCCATTGCTTTCTGTGCTAAAATAGGAAATGGAAAATGCACCAACTTTTTACATATGAAGGAACTGATTACTCTTTGATGGGAGTTTTTTTTTTCTTGATTGAATTTGTAAATTCAGAAAGCGATTTTAGATATGTCAGTCATAGGAAAACAAAATGTACTTTCAGCACTGAGTTTTTCATGCCTTTACCCAAACAGCTTAATATTTTGAGATTGATGGTTTATGATGTGTGGTATCGAAGCCTCTATAACCATGTGGTTTGGAGTTGGGTTCTTTTTTCCCCATTCTTCCAAATGAACTTTCAACATAAGGTGGTCATGTGTTGTCCATGCTTCAAGCGCAAAGGCCTCTTGTATGAGGGGCATGTCAAACATGAAAGAGCTTAATGTTTCTGAGATGATTTATGAACAATCGAACATATTCAACCGGCAACAATATACTAAAAATCTAAGCTGATTTAAAAGCATTATACTTGTTTGATGATTTTTATATAGAGGAATGTTATTTGAACAACCATTTTATGGGACAACTTATGAGACAACCAAAATATACAAAGAAATGTAGGTTTTGACACAAAAACCAAAGCAATAGAGAGAGAAAATGAGAACATAATGTGAGTATGAGAGAAAAAGTTGTCATAAAAGTTGTCACAAAATGGTTGTTCAAATATCATTTCTCTTTTATATACATCAATAAATAATTTAGCAATATTTTGTCTTGTATGCAATTGACCACTGAGATTTATGACATTTTTTTTGAATATTTTTTATATTATTTGGTTGATAAGCACCCACTGGTTCATGTGACTTGTAGGTAATACTTCACTCTGAAGTAAAGGCTGTGTTGACCATATACGATGCTTGGCTTGATCTTCAAGATGGATTTGTTCATTCTGGACAAACTGAGGGTAGACCTAAATCAAGCTTCTTCCCACTAATCATATCTCCCACTTCCAAAGCAGGAATTTTGTTTA

Protein sequence

>MS.gene059872.t1

MRTIALKLEFGAFHNQIFERTLAVHFTHPFYVRTRVTDKCNDGTLLLQVILHSEVKAVLTIYDAWLDLQDGFVHSGQTEGRPKSSFFPLIISPTSKAGILF