AlfalfaGEDB | MS.gene059362

M. sativa cultivar XinJiangDaYe / MS.gene059362

KEGG: K01251
GO: GO:0003674 GO:0003824 GO:0004013 GO:0016787 GO:0016801 GO:0016802
PFAM: PF05221.20

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene059362.t1	XP_003629610.1	100	202	0	0	1	202	36	237	1.40E-112	415.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene059362.t1	P50246	94.6	202	11	0	1	202	36	237	2.4e-110	399.4

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene059362.t1	Q84RD9	100.0	202	0	0	1	202	36	237	9.8e-113	415.6

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene059362	MS.gene059364	0.908795	1.16E-81	-1.69E-46
MS.gene059362	MS.gene071710	0.822816	1.93E-53	-1.69E-46
MS.gene059362	MS.gene071711	0.881546	2.19E-70	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene059362.t1	MTR_8g083090	100.000	202	0	0	1	202	36	237	1.31e-148	422
MS.gene059362.t1	MTR_3g084340	93.564	202	13	0	1	202	36	237	1.21e-131	379

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene059362.t1	AT4G13940	91.584	202	17	0	1	202	36	237	2.31e-131	372
MS.gene059362.t1	AT4G13940	91.584	202	17	0	1	202	36	237	3.20e-130	375
MS.gene059362.t1	AT3G23810	89.109	202	22	0	1	202	36	237	5.19e-125	362
MS.gene059362.t1	AT4G13940	69.307	202	17	1	1	202	36	192	6.53e-88	266
MS.gene059362.t1	AT4G13940	86.087	115	16	0	88	202	2	116	2.65e-68	213

Find 68 sgRNAs with CRISPR-Local

Find 68 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GTACTGAACGTGCTCTTGAC+TGG	0.316338	4.2:+13275050	MS.gene059362:CDS
TTGAAGAGTCTCACCCTTCC+AGG	0.334073	4.2:-13275014	None:intergenic
TTCATCTTACGATACCTCTT+AGG	0.348879	4.2:-13275256	None:intergenic
ATCGAAACCCTAACCGCTTT+AGG	0.353133	4.2:+13274902	MS.gene059362:CDS
GGTGATTCTTGCACCTTTGA+AGG	0.359405	4.2:-13274843	None:intergenic
TTCTTGCACCTTTGAAGGGT+TGG	0.381461	4.2:-13274838	None:intergenic
CTTCAGCACCTAAAGCGGTT+AGG	0.385562	4.2:-13274910	None:intergenic
TACTGAACGTGCTCTTGACT+GGG	0.392027	4.2:+13275051	MS.gene059362:CDS
TCTTGCACCTTTGAAGGGTT+GGG	0.392418	4.2:-13274837	None:intergenic
CGTGCTCTTGACTGGGGTCC+AGG	0.395326	4.2:+13275058	MS.gene059362:CDS
ACAGCGCCGCCGTCTTCGCC+TGG	0.403265	4.2:+13274996	MS.gene059362:CDS
GTCACGAGCGATGGCGGCGG+CGG	0.418277	4.2:-13274975	None:intergenic
CGCCGCCGTCTTCGCCTGGA+AGG	0.420226	4.2:+13275000	MS.gene059362:CDS
TTTCGATGAGGACGGCGGTT+TGG	0.421389	4.2:-13274886	None:intergenic
TTCAGCACCTAAAGCGGTTA+GGG	0.426813	4.2:-13274909	None:intergenic
TTGAGTTGAGAAAATGTTGC+AGG	0.426919	4.2:-13274945	None:intergenic
CTTGACTGGGGTCCAGGTGG+TGG	0.427852	4.2:+13275064	MS.gene059362:CDS
GTTGATCCACGAAGGTGTTA+AGG	0.434225	4.2:+13275126	MS.gene059362:CDS
AGTTCGGCAGGACATGAGTC+CGG	0.444561	4.2:-13274801	None:intergenic
ATGTCCTGCCGAACTGAGTT+CGG	0.451578	4.2:+13274809	MS.gene059362:CDS
CTTCAGCCTTAACACCTTCG+TGG	0.456708	4.2:-13275132	None:intergenic
GAGCGATGGCGGCGGCGGCG+TGG	0.480226	4.2:-13274970	None:intergenic
GTGCTTACTATCATTAGAGA+TGG	0.486117	4.2:+13275220	MS.gene059362:CDS
GATGAGGACGGCGGTTTGGA+TGG	0.488837	4.2:-13274882	None:intergenic
AAGATGAAGGATAGACTTGT+TGG	0.490166	4.2:+13275271	MS.gene059362:CDS
TGCTTACTATCATTAGAGAT+GGG	0.504002	4.2:+13275221	MS.gene059362:CDS
TGGTCCGAACTCAGTTCGGC+AGG	0.506633	4.2:-13274813	None:intergenic
GCACCTTTGAAGGGTTGGGA+TGG	0.510136	4.2:-13274833	None:intergenic
GTGATTCTTGCACCTTTGAA+GGG	0.510838	4.2:-13274842	None:intergenic
AGACTACTACTGGTGTTAAG+AGG	0.510864	4.2:+13275305	MS.gene059362:CDS
GGGATGGTCCGAACTCAGTT+CGG	0.513848	4.2:-13274817	None:intergenic
GGCGCTGTCACGAGCGATGG+CGG	0.513966	4.2:-13274981	None:intergenic
GTTCGGCAGGACATGAGTCC+GGG	0.520546	4.2:-13274800	None:intergenic
GTGAGACTCTTCAAGAGTAT+TGG	0.531338	4.2:+13275023	MS.gene059362:CDS
GAGTCGTTGACATTAATAGC+AGG	0.532386	4.2:-13275364	None:intergenic
GCTGTCACGAGCGATGGCGG+CGG	0.534560	4.2:-13274978	None:intergenic
TTCAAAGGTGCAAGAATCAC+CGG	0.538038	4.2:+13274845	MS.gene059362:CDS
CTTTAGGTGCTGAAGTCCGT+TGG	0.543805	4.2:+13274918	MS.gene059362:CDS
TTATCAGTAGAACTTGGATC+AGG	0.545116	4.2:-13275181	None:intergenic
GAAGAACTTTATGAGAAGAC+TGG	0.546774	4.2:+13275151	MS.gene059362:CDS
CCATCGTCAACAATCAAGTC+AGG	0.547026	4.2:-13275088	None:intergenic
AGACTCTTCAAGAGTATTGG+TGG	0.548092	4.2:+13275026	MS.gene059362:CDS
TCAGCGTTATCAGTAGAACT+TGG	0.550511	4.2:-13275187	None:intergenic
ATCAAGTCAGGACCACCACC+TGG	0.553340	4.2:-13275076	None:intergenic
CTTTATCAGATGCAAGCTAG+TGG	0.573265	4.2:+13275328	MS.gene059362:CDS
GATGGTCATGTGAAGAGATC+CGG	0.575220	4.2:-13274864	None:intergenic
GGTTGAAGACTGATCCTAAG+AGG	0.575943	4.2:+13275242	MS.gene059362:CDS
GGCGGCGCTGTCACGAGCGA+TGG	0.576329	4.2:-13274984	None:intergenic
TAAGAGGTATCGTAAGATGA+AGG	0.581482	4.2:+13275258	MS.gene059362:CDS
CTCACCCTTCCAGGCGAAGA+CGG	0.582229	4.2:-13275005	None:intergenic
TTGGCCGAAATCGAGATGCC+CGG	0.583401	4.2:+13274782	None:intergenic
GCCGCCGTCTTCGCCTGGAA+GGG	0.586397	4.2:+13275001	MS.gene059362:CDS
GGACCATCCCAACCCTTCAA+AGG	0.588552	4.2:+13274830	MS.gene059362:CDS
GTTTCTGAAGAGACTACTAC+TGG	0.608136	4.2:+13275295	MS.gene059362:CDS
CGACTCTGTTACCAAGAGCA+AGG	0.612040	4.2:+13275381	MS.gene059362:CDS
ACCCTTCCAGGCGAAGACGG+CGG	0.621848	4.2:-13275002	None:intergenic
GGTTAGGGTTTCGATGAGGA+CGG	0.624245	4.2:-13274894	None:intergenic
AAATGTTGCAGGAACACCAA+CGG	0.640245	4.2:-13274934	None:intergenic
ACGGACTTCAGCACCTAAAG+CGG	0.651771	4.2:-13274915	None:intergenic
AGTCGTTGACATTAATAGCA+GGG	0.657078	4.2:-13275363	None:intergenic
GCTCTTGACTGGGGTCCAGG+TGG	0.660501	4.2:+13275061	MS.gene059362:CDS
ACTTTATGAGAAGACTGGTG+AGG	0.668763	4.2:+13275156	MS.gene059362:CDS
GACTTGATTGTTGACGATGG+TGG	0.670668	4.2:+13275091	MS.gene059362:CDS
CCTGACTTGATTGTTGACGA+TGG	0.671777	4.2:+13275088	MS.gene059362:CDS
TAGGGTTTCGATGAGGACGG+CGG	0.676084	4.2:-13274891	None:intergenic
ACTGAACGTGCTCTTGACTG+GGG	0.690263	4.2:+13275052	MS.gene059362:CDS
AAGCGGTTAGGGTTTCGATG+AGG	0.719864	4.2:-13274898	None:intergenic
GCTACTTTGTTGATCCACGA+AGG	0.723062	4.2:+13275118	MS.gene059362:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AAAAAAACAAACCTTGCTCT+TGG	-	chr4.2:13275395-13275414	None:intergenic	30.0%
	TGCTTACTATCATTAGAGAT+GGG	+	chr4.2:13275221-13275240	MS.gene059362:CDS	30.0%
	AAGATGAAGGATAGACTTGT+TGG	+	chr4.2:13275271-13275290	MS.gene059362:CDS	35.0%
	AGTCGTTGACATTAATAGCA+GGG	-	chr4.2:13275366-13275385	None:intergenic	35.0%
	GAAGAACTTTATGAGAAGAC+TGG	+	chr4.2:13275151-13275170	MS.gene059362:CDS	35.0%
	GTGCTTACTATCATTAGAGA+TGG	+	chr4.2:13275220-13275239	MS.gene059362:CDS	35.0%
	TTATCAGTAGAACTTGGATC+AGG	-	chr4.2:13275184-13275203	None:intergenic	35.0%
	TTCATCTTACGATACCTCTT+AGG	-	chr4.2:13275259-13275278	None:intergenic	35.0%
!!	TAAGAGGTATCGTAAGATGA+AGG	+	chr4.2:13275258-13275277	MS.gene059362:CDS	35.0%
!!	TTGAGTTGAGAAAATGTTGC+AGG	-	chr4.2:13274948-13274967	None:intergenic	35.0%
	AAATGTTGCAGGAACACCAA+CGG	-	chr4.2:13274937-13274956	None:intergenic	40.0%
	ACTTTATGAGAAGACTGGTG+AGG	+	chr4.2:13275156-13275175	MS.gene059362:CDS	40.0%
	CTTTATCAGATGCAAGCTAG+TGG	+	chr4.2:13275328-13275347	MS.gene059362:CDS	40.0%
	GAGTCGTTGACATTAATAGC+AGG	-	chr4.2:13275367-13275386	None:intergenic	40.0%
	GTGATTCTTGCACCTTTGAA+GGG	-	chr4.2:13274845-13274864	None:intergenic	40.0%
	TCAGCGTTATCAGTAGAACT+TGG	-	chr4.2:13275190-13275209	None:intergenic	40.0%
!	AGACTACTACTGGTGTTAAG+AGG	+	chr4.2:13275305-13275324	MS.gene059362:CDS	40.0%
!	GTGAGACTCTTCAAGAGTAT+TGG	+	chr4.2:13275023-13275042	MS.gene059362:CDS	40.0%
!	GTTTCTGAAGAGACTACTAC+TGG	+	chr4.2:13275295-13275314	MS.gene059362:CDS	40.0%
!	TTCAAAGGTGCAAGAATCAC+CGG	+	chr4.2:13274845-13274864	MS.gene059362:CDS	40.0%
!!	AGACTCTTCAAGAGTATTGG+TGG	+	chr4.2:13275026-13275045	MS.gene059362:CDS	40.0%
	ATCGAAACCCTAACCGCTTT+AGG	+	chr4.2:13274902-13274921	MS.gene059362:CDS	45.0%
	CCATCGTCAACAATCAAGTC+AGG	-	chr4.2:13275091-13275110	None:intergenic	45.0%
	GACTTGATTGTTGACGATGG+TGG	+	chr4.2:13275091-13275110	MS.gene059362:CDS	45.0%
	GATGGTCATGTGAAGAGATC+CGG	-	chr4.2:13274867-13274886	None:intergenic	45.0%
	GGTGATTCTTGCACCTTTGA+AGG	-	chr4.2:13274846-13274865	None:intergenic	45.0%
	GGTTGAAGACTGATCCTAAG+AGG	+	chr4.2:13275242-13275261	MS.gene059362:CDS	45.0%
	TACTGAACGTGCTCTTGACT+GGG	+	chr4.2:13275051-13275070	MS.gene059362:CDS	45.0%
	TTCAGCACCTAAAGCGGTTA+GGG	-	chr4.2:13274912-13274931	None:intergenic	45.0%
!	CCTGACTTGATTGTTGACGA+TGG	+	chr4.2:13275088-13275107	MS.gene059362:CDS	45.0%
!	GCTACTTTGTTGATCCACGA+AGG	+	chr4.2:13275118-13275137	MS.gene059362:CDS	45.0%
!	TCTTGCACCTTTGAAGGGTT+GGG	-	chr4.2:13274840-13274859	None:intergenic	45.0%
!	TTCTTGCACCTTTGAAGGGT+TGG	-	chr4.2:13274841-13274860	None:intergenic	45.0%
!!	GTTGATCCACGAAGGTGTTA+AGG	+	chr4.2:13275126-13275145	MS.gene059362:CDS	45.0%
	AAGCGGTTAGGGTTTCGATG+AGG	-	chr4.2:13274901-13274920	None:intergenic	50.0%
	ACGGACTTCAGCACCTAAAG+CGG	-	chr4.2:13274918-13274937	None:intergenic	50.0%
	ACTGAACGTGCTCTTGACTG+GGG	+	chr4.2:13275052-13275071	MS.gene059362:CDS	50.0%
	ATGTCCTGCCGAACTGAGTT+CGG	+	chr4.2:13274809-13274828	MS.gene059362:CDS	50.0%
	CGACTCTGTTACCAAGAGCA+AGG	+	chr4.2:13275381-13275400	MS.gene059362:CDS	50.0%
	CTTCAGCACCTAAAGCGGTT+AGG	-	chr4.2:13274913-13274932	None:intergenic	50.0%
	CTTCAGCCTTAACACCTTCG+TGG	-	chr4.2:13275135-13275154	None:intergenic	50.0%
	GGTTAGGGTTTCGATGAGGA+CGG	-	chr4.2:13274897-13274916	None:intergenic	50.0%
	GTACTGAACGTGCTCTTGAC+TGG	+	chr4.2:13275050-13275069	MS.gene059362:CDS	50.0%
	TTGAAGAGTCTCACCCTTCC+AGG	-	chr4.2:13275017-13275036	None:intergenic	50.0%
!!	CTTTAGGTGCTGAAGTCCGT+TGG	+	chr4.2:13274918-13274937	MS.gene059362:CDS	50.0%
	AGTTCGGCAGGACATGAGTC+CGG	-	chr4.2:13274804-13274823	None:intergenic	55.0%
	ATCAAGTCAGGACCACCACC+TGG	-	chr4.2:13275079-13275098	None:intergenic	55.0%
	GGACCATCCCAACCCTTCAA+AGG	+	chr4.2:13274830-13274849	MS.gene059362:CDS	55.0%
	GGGATGGTCCGAACTCAGTT+CGG	-	chr4.2:13274820-13274839	None:intergenic	55.0%
	TAGGGTTTCGATGAGGACGG+CGG	-	chr4.2:13274894-13274913	None:intergenic	55.0%
	TTTCGATGAGGACGGCGGTT+TGG	-	chr4.2:13274889-13274908	None:intergenic	55.0%
!	GCACCTTTGAAGGGTTGGGA+TGG	-	chr4.2:13274836-13274855	None:intergenic	55.0%
	CTCACCCTTCCAGGCGAAGA+CGG	-	chr4.2:13275008-13275027	None:intergenic	60.0%
	GTTCGGCAGGACATGAGTCC+GGG	-	chr4.2:13274803-13274822	None:intergenic	60.0%
	TGGTCCGAACTCAGTTCGGC+AGG	-	chr4.2:13274816-13274835	None:intergenic	60.0%
!	GATGAGGACGGCGGTTTGGA+TGG	-	chr4.2:13274885-13274904	None:intergenic	60.0%
	ACCCTTCCAGGCGAAGACGG+CGG	-	chr4.2:13275005-13275024	None:intergenic	65.0%
	CGTGCTCTTGACTGGGGTCC+AGG	+	chr4.2:13275058-13275077	MS.gene059362:CDS	65.0%
	CTTGACTGGGGTCCAGGTGG+TGG	+	chr4.2:13275064-13275083	MS.gene059362:CDS	65.0%
	GCTCTTGACTGGGGTCCAGG+TGG	+	chr4.2:13275061-13275080	MS.gene059362:CDS	65.0%
	GCCGCCGTCTTCGCCTGGAA+GGG	+	chr4.2:13275001-13275020	MS.gene059362:CDS	70.0%
	GGCGCTGTCACGAGCGATGG+CGG	-	chr4.2:13274984-13275003	None:intergenic	70.0%
!	GCTGTCACGAGCGATGGCGG+CGG	-	chr4.2:13274981-13275000	None:intergenic	70.0%
	ACAGCGCCGCCGTCTTCGCC+TGG	+	chr4.2:13274996-13275015	MS.gene059362:CDS	75.0%
	CGCCGCCGTCTTCGCCTGGA+AGG	+	chr4.2:13275000-13275019	MS.gene059362:CDS	75.0%
	GGCGGCGCTGTCACGAGCGA+TGG	-	chr4.2:13274987-13275006	None:intergenic	75.0%
!	GTCACGAGCGATGGCGGCGG+CGG	-	chr4.2:13274978-13274997	None:intergenic	75.0%
!!	GAGCGATGGCGGCGGCGGCG+TGG	-	chr4.2:13274973-13274992	None:intergenic	85.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.2	gene	13274797	13275429	13274797	ID=MS.gene059362
chr4.2	mRNA	13274797	13275429	13274797	ID=MS.gene059362.t1;Parent=MS.gene059362
chr4.2	exon	13274797	13275429	13274797	ID=MS.gene059362.t1.exon1;Parent=MS.gene059362.t1
chr4.2	CDS	13274797	13275429	13274797	ID=cds.MS.gene059362.t1;Parent=MS.gene059362.t1

Gene Sequence

>MS.gene059362

ATGCCCGGACTCATGTCCTGCCGAACTGAGTTCGGACCATCCCAACCCTTCAAAGGTGCAAGAATCACCGGATCTCTTCACATGACCATCCAAACCGCCGTCCTCATCGAAACCCTAACCGCTTTAGGTGCTGAAGTCCGTTGGTGTTCCTGCAACATTTTCTCAACTCAAGACCACGCCGCCGCCGCCATCGCTCGTGACAGCGCCGCCGTCTTCGCCTGGAAGGGTGAGACTCTTCAAGAGTATTGGTGGTGTACTGAACGTGCTCTTGACTGGGGTCCAGGTGGTGGTCCTGACTTGATTGTTGACGATGGTGGTGATGCTACTTTGTTGATCCACGAAGGTGTTAAGGCTGAAGAACTTTATGAGAAGACTGGTGAGGTTCCTGATCCAAGTTCTACTGATAACGCTGAGTTTCAGCTTGTGCTTACTATCATTAGAGATGGGTTGAAGACTGATCCTAAGAGGTATCGTAAGATGAAGGATAGACTTGTTGGTGTTTCTGAAGAGACTACTACTGGTGTTAAGAGGCTTTATCAGATGCAAGCTAGTGGAACTCTTTTGTTCCCTGCTATTAATGTCAACGACTCTGTTACCAAGAGCAAGGTTTGTTTTTTTTTTTTTTTTTTGTAA

Protein sequence

>MS.gene059362.t1

MPGLMSCRTEFGPSQPFKGARITGSLHMTIQTAVLIETLTALGAEVRWCSCNIFSTQDHAAAAIARDSAAVFAWKGETLQEYWWCTERALDWGPGGGPDLIVDDGGDATLLIHEGVKAEELYEKTGEVPDPSSTDNAEFQLVLTIIRDGLKTDPKRYRKMKDRLVGVSEETTTGVKRLYQMQASGTLLFPAINVNDSVTKSKVCFFFFFL