Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071739.t1 | PNY14304.1 | 88.8 | 161 | 18 | 0 | 1 | 161 | 91 | 251 | 1.10E-74 | 289.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071739.t1 | Q9SX33 | 73.9 | 161 | 42 | 0 | 1 | 161 | 85 | 245 | 2.2e-63 | 243.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071739.t1 | A0A2K3PGC8 | 88.8 | 161 | 18 | 0 | 1 | 161 | 91 | 251 | 8.2e-75 | 289.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052463 | MS.gene071739 | 0.813841 | 2.09E-51 | -1.69E-46 |
| MS.gene054576 | MS.gene071739 | 0.818276 | 2.13E-52 | -1.69E-46 |
| MS.gene059439 | MS.gene071739 | 0.808974 | 2.38E-50 | -1.69E-46 |
| MS.gene061100 | MS.gene071739 | 0.800109 | 1.68E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 27 sgRNAs with CRISPR-Local
Find 80 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTCCTCTTGTTGTTGTTAT+TGG | 0.033009 | 8.3:-32683962 | MS.gene071739:CDS |
| TACTCGTTTGTTGGGGAGCT+TGG | 0.313180 | 8.3:-32682654 | MS.gene071739:CDS |
| TGTCTCACCGTCCAAGTTCA+TGG | 0.336386 | 8.3:+32682785 | None:intergenic |
| GTTAAAGTTCATAAAGTTGA+TGG | 0.354361 | 8.3:-32682939 | MS.gene071739:CDS |
| CAAATCTGTACTCGTTTGTT+GGG | 0.362936 | 8.3:-32682662 | MS.gene071739:CDS |
| GCAAATCTGTACTCGTTTGT+TGG | 0.405864 | 8.3:-32682663 | MS.gene071739:CDS |
| TTGGGGAGCTTGGAGTTTGA+AGG | 0.406920 | 8.3:-32682644 | None:intergenic |
| TGAAAGAAGGTATTGAAGAT+TGG | 0.406954 | 8.3:-32683928 | MS.gene071739:CDS |
| ATAGCAGTAACAGCTCTATA+AGG | 0.412735 | 8.3:+32683986 | None:intergenic |
| AACGAGTACAGATTTGCATT+TGG | 0.420298 | 8.3:+32682669 | None:intergenic |
| GAAAGCAATAAGATATCTGC+TGG | 0.454136 | 8.3:+32682843 | None:intergenic |
| ATTGCTTTCGTCGAGTTATG+AGG | 0.492509 | 8.3:-32682829 | MS.gene071739:CDS |
| TTATGTTGAGACCATGAACT+TGG | 0.501641 | 8.3:-32682796 | MS.gene071739:CDS |
| GATTCTCCTGCAGGATATTG+AGG | 0.509618 | 8.3:-32682976 | MS.gene071739:intron |
| AAATATAGTGAAGGTAGAGA+AGG | 0.509951 | 8.3:-32682877 | MS.gene071739:CDS |
| AAGGTATTGAAGATTGGCGT+AGG | 0.553334 | 8.3:-32683922 | MS.gene071739:CDS |
| GGAGCAACTATTGTGAAAGA+AGG | 0.556916 | 8.3:-32683941 | MS.gene071739:CDS |
| TGAAGTTGAGATCCTCATGT+AGG | 0.564006 | 8.3:+32682724 | None:intergenic |
| GCTCCAATAACAACAACAAG+AGG | 0.592389 | 8.3:+32683959 | None:intergenic |
| GAAGGTAGGAAATATAGTGA+AGG | 0.597992 | 8.3:-32682886 | MS.gene071739:CDS |
| CATGGGAAGAATCTGAAGGT+AGG | 0.610508 | 8.3:-32682900 | MS.gene071739:CDS |
| CAATAACAACAACAAGAGGA+AGG | 0.622130 | 8.3:+32683963 | None:intergenic |
| AAATCTGTACTCGTTTGTTG+GGG | 0.631527 | 8.3:-32682661 | MS.gene071739:CDS |
| CGGTAACATCTTCCTACATG+AGG | 0.643470 | 8.3:-32682736 | MS.gene071739:CDS |
| AAAGCAATAAGATATCTGCT+GGG | 0.653943 | 8.3:+32682844 | None:intergenic |
| GTTGAGACCATGAACTTGGA+CGG | 0.659530 | 8.3:-32682792 | MS.gene071739:CDS |
| ACTGCATGGGAAGAATCTGA+AGG | 0.668548 | 8.3:-32682904 | MS.gene071739:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTGATGTTAATAAAATATAA+GGG | - | chr8.3:32682962-32682981 | MS.gene071739:CDS | 10.0% |
| !! | TTTGATGTTAATAAAATATA+AGG | - | chr8.3:32682961-32682980 | MS.gene071739:CDS | 10.0% |
| !!! | AAATTGTTATTTTTAATCAA+TGG | - | chr8.3:32683567-32683586 | MS.gene071739:intron | 10.0% |
| !! | AAAATTCAATCCAAATTTAA+TGG | - | chr8.3:32683238-32683257 | MS.gene071739:intron | 15.0% |
| !! | TTCAAATATTGAACATATAT+TGG | + | chr8.3:32683448-32683467 | None:intergenic | 15.0% |
| !!! | TTAACTAATTGAAAATTTTG+TGG | - | chr8.3:32683279-32683298 | MS.gene071739:intron | 15.0% |
| !! | ACATAATACAAGAAAATCAT+TGG | + | chr8.3:32683539-32683558 | None:intergenic | 20.0% |
| !! | ATAAGGGAGATAAATTATTT+AGG | - | chr8.3:32682978-32682997 | MS.gene071739:intron | 20.0% |
| !! | ATTCTTTAAGCCATTAAATT+TGG | + | chr8.3:32683251-32683270 | None:intergenic | 20.0% |
| !!! | AATTTCTATTTTTTGGTTAC+TGG | - | chr8.3:32682657-32682676 | MS.gene071739:CDS | 20.0% |
| !!! | AATTTTGTGGTATTTTTACA+AGG | - | chr8.3:32683292-32683311 | MS.gene071739:intron | 20.0% |
| !!! | AGTTGCAAATTTCTATTTTT+TGG | - | chr8.3:32682650-32682669 | MS.gene071739:CDS | 20.0% |
| ! | ACAACAGATTATAGCTTATA+AGG | + | chr8.3:32683121-32683140 | None:intergenic | 25.0% |
| ! | ACAGAATATTCTGATTCATA+TGG | + | chr8.3:32682917-32682936 | None:intergenic | 25.0% |
| ! | CTTTACAAAAAGGTAAATTG+TGG | - | chr8.3:32683325-32683344 | MS.gene071739:intron | 25.0% |
| ! | GTTAAAGTTCATAAAGTTGA+TGG | - | chr8.3:32683752-32683771 | MS.gene071739:intron | 25.0% |
| ! | TAAATTATTTAGGCTCTGTT+TGG | - | chr8.3:32682988-32683007 | MS.gene071739:intron | 25.0% |
| !! | ACAAATTTGAAGCAAAAACA+AGG | - | chr8.3:32683926-32683945 | MS.gene071739:CDS | 25.0% |
| !! | TCTGTATTTTATCATATGCA+GGG | + | chr8.3:32683623-32683642 | None:intergenic | 25.0% |
| !!! | AATTTTTTGAGCTTATCAAC+AGG | + | chr8.3:32683224-32683243 | None:intergenic | 25.0% |
| AAAGCAATAAGATATCTGCT+GGG | + | chr8.3:32683850-32683869 | None:intergenic | 30.0% | |
| AAATATAGTGAAGGTAGAGA+AGG | - | chr8.3:32683814-32683833 | MS.gene071739:intron | 30.0% | |
| AAGCAAAAACAAGGTTTAGA+CGG | - | chr8.3:32683935-32683954 | MS.gene071739:CDS | 30.0% | |
| ! | CCATGAAGTACTTTACAAAA+AGG | - | chr8.3:32683315-32683334 | MS.gene071739:intron | 30.0% |
| ! | CTCTGTATTTTATCATATGC+AGG | + | chr8.3:32683624-32683643 | None:intergenic | 30.0% |
| ! | TACAGAGTTAGTAATGCTTT+TGG | - | chr8.3:32683637-32683656 | MS.gene071739:intron | 30.0% |
| !! | AGCTTTTCTGTTTAGTTTCT+CGG | - | chr8.3:32683370-32683389 | MS.gene071739:intron | 30.0% |
| !! | CCTTTTTGTAAAGTACTTCA+TGG | + | chr8.3:32683318-32683337 | None:intergenic | 30.0% |
| !! | TGAAAGAAGGTATTGAAGAT+TGG | - | chr8.3:32682763-32682782 | MS.gene071739:CDS | 30.0% |
| !!! | TTTTTTGGTTACTGGTGTTT+TGG | - | chr8.3:32682665-32682684 | MS.gene071739:CDS | 30.0% |
| AACGAGTACAGATTTGCATT+TGG | + | chr8.3:32684025-32684044 | None:intergenic | 35.0% | |
| ACAAAAAGGTAAATTGTGGC+AGG | - | chr8.3:32683329-32683348 | MS.gene071739:intron | 35.0% | |
| ATAGCAGTAACAGCTCTATA+AGG | + | chr8.3:32682708-32682727 | None:intergenic | 35.0% | |
| ATTTGAATCTGCTAAGTTGC+AGG | - | chr8.3:32683461-32683480 | MS.gene071739:intron | 35.0% | |
| CAAATCTGTACTCGTTTGTT+GGG | - | chr8.3:32684029-32684048 | MS.gene071739:CDS | 35.0% | |
| CAATAACAACAACAAGAGGA+AGG | + | chr8.3:32682731-32682750 | None:intergenic | 35.0% | |
| GAAAGCAATAAGATATCTGC+TGG | + | chr8.3:32683851-32683870 | None:intergenic | 35.0% | |
| GAAGGTAGGAAATATAGTGA+AGG | - | chr8.3:32683805-32683824 | MS.gene071739:intron | 35.0% | |
| GTAACCCCTAAAAAACTTGA+GGG | - | chr8.3:32683511-32683530 | MS.gene071739:intron | 35.0% | |
| TGTAACCCCTAAAAAACTTG+AGG | - | chr8.3:32683510-32683529 | MS.gene071739:intron | 35.0% | |
| TTATGTTGAGACCATGAACT+TGG | - | chr8.3:32683895-32683914 | MS.gene071739:intron | 35.0% | |
| TTTCTCGGTATTGTTTCGAA+AGG | - | chr8.3:32683385-32683404 | MS.gene071739:intron | 35.0% | |
| TTTGAATCTGCTAAGTTGCA+GGG | - | chr8.3:32683462-32683481 | MS.gene071739:intron | 35.0% | |
| ! | AAATCTGTACTCGTTTGTTG+GGG | - | chr8.3:32684030-32684049 | MS.gene071739:CDS | 35.0% |
| ! | GCGTTTTTGATATACTGCAT+GGG | - | chr8.3:32683774-32683793 | MS.gene071739:intron | 35.0% |
| !! | CTTCCTCTTGTTGTTGTTAT+TGG | - | chr8.3:32682729-32682748 | MS.gene071739:CDS | 35.0% |
| AAACACTCGTGAGACTATTC+AGG | + | chr8.3:32683198-32683217 | None:intergenic | 40.0% | |
| AACACTCGTGAGACTATTCA+GGG | + | chr8.3:32683197-32683216 | None:intergenic | 40.0% | |
| ACTACCAACTCTTTCTGTTG+TGG | - | chr8.3:32682818-32682837 | MS.gene071739:CDS | 40.0% | |
| AGACTATTCAGGGGAGATTA+TGG | + | chr8.3:32683187-32683206 | None:intergenic | 40.0% | |
| ATTGCTTTCGTCGAGTTATG+AGG | - | chr8.3:32683862-32683881 | MS.gene071739:intron | 40.0% | |
| GAATCCACAACAGAAAGAGT+TGG | + | chr8.3:32682825-32682844 | None:intergenic | 40.0% | |
| GACATATATGATTCTCCTGC+AGG | - | chr8.3:32683706-32683725 | MS.gene071739:intron | 40.0% | |
| GCAAATCTGTACTCGTTTGT+TGG | - | chr8.3:32684028-32684047 | MS.gene071739:CDS | 40.0% | |
| GCTCCAATAACAACAACAAG+AGG | + | chr8.3:32682735-32682754 | None:intergenic | 40.0% | |
| GGAGCAACTATTGTGAAAGA+AGG | - | chr8.3:32682750-32682769 | MS.gene071739:CDS | 40.0% | |
| TGAAGTTGAGATCCTCATGT+AGG | + | chr8.3:32683970-32683989 | None:intergenic | 40.0% | |
| ! | GGCGTTTTTGATATACTGCA+TGG | - | chr8.3:32683773-32683792 | MS.gene071739:intron | 40.0% |
| !! | AAGGTATTGAAGATTGGCGT+AGG | - | chr8.3:32682769-32682788 | MS.gene071739:CDS | 40.0% |
| !!! | TGGCTCCCTCAAGTTTTTTA+GGG | + | chr8.3:32683519-32683538 | None:intergenic | 40.0% |
| !!! | TTGGCTCCCTCAAGTTTTTT+AGG | + | chr8.3:32683520-32683539 | None:intergenic | 40.0% |
| AAATTGTGGCAGGCAGCTTA+TGG | - | chr8.3:32683339-32683358 | MS.gene071739:intron | 45.0% | |
| AAGCTATCCCAGAGAGCTTA+TGG | - | chr8.3:32683144-32683163 | MS.gene071739:intron | 45.0% | |
| ACACTCGTGAGACTATTCAG+GGG | + | chr8.3:32683196-32683215 | None:intergenic | 45.0% | |
| ACTGCATGGGAAGAATCTGA+AGG | - | chr8.3:32683787-32683806 | MS.gene071739:intron | 45.0% | |
| AGATTGGCGTAGGAAAAAGC+AGG | - | chr8.3:32682779-32682798 | MS.gene071739:CDS | 45.0% | |
| CATGGGAAGAATCTGAAGGT+AGG | - | chr8.3:32683791-32683810 | MS.gene071739:intron | 45.0% | |
| CGGTAACATCTTCCTACATG+AGG | - | chr8.3:32683955-32683974 | MS.gene071739:CDS | 45.0% | |
| GACATGTCCATAAGCTCTCT+GGG | + | chr8.3:32683154-32683173 | None:intergenic | 45.0% | |
| GATTCTCCTGCAGGATATTG+AGG | - | chr8.3:32683715-32683734 | MS.gene071739:intron | 45.0% | |
| GTACTGCAGGATAGAGAAGA+AGG | + | chr8.3:32682869-32682888 | None:intergenic | 45.0% | |
| GTTGAGACCATGAACTTGGA+CGG | - | chr8.3:32683899-32683918 | MS.gene071739:intron | 45.0% | |
| TCTCTACACACTTGTACTGC+AGG | + | chr8.3:32682882-32682901 | None:intergenic | 45.0% | |
| TGACATGTCCATAAGCTCTC+TGG | + | chr8.3:32683155-32683174 | None:intergenic | 45.0% | |
| TGTTCACCTCAATATCCTGC+AGG | + | chr8.3:32683724-32683743 | None:intergenic | 45.0% | |
| !!! | GGCTCCCTCAAGTTTTTTAG+GGG | + | chr8.3:32683518-32683537 | None:intergenic | 45.0% |
| AAGTTGCAGGGTGCACGTAT+AGG | - | chr8.3:32683474-32683493 | MS.gene071739:intron | 50.0% | |
| AGTTGCAGGGTGCACGTATA+GGG | - | chr8.3:32683475-32683494 | MS.gene071739:intron | 50.0% | |
| TGTCTCACCGTCCAAGTTCA+TGG | + | chr8.3:32683909-32683928 | None:intergenic | 50.0% | |
| ! | TACTCGTTTGTTGGGGAGCT+TGG | - | chr8.3:32684037-32684056 | MS.gene071739:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 32682647 | 32684066 | 32682647 | ID=MS.gene071739 |
| chr8.3 | mRNA | 32682647 | 32684066 | 32682647 | ID=MS.gene071739.t1;Parent=MS.gene071739 |
| chr8.3 | exon | 32683913 | 32684066 | 32683913 | ID=MS.gene071739.t1.exon1;Parent=MS.gene071739.t1 |
| chr8.3 | CDS | 32683913 | 32684066 | 32683913 | ID=cds.MS.gene071739.t1;Parent=MS.gene071739.t1 |
| chr8.3 | exon | 32682647 | 32682985 | 32682647 | ID=MS.gene071739.t1.exon2;Parent=MS.gene071739.t1 |
| chr8.3 | CDS | 32682647 | 32682985 | 32682647 | ID=cds.MS.gene071739.t1;Parent=MS.gene071739.t1 |
| Gene Sequence |
| Protein sequence |
