AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene071859

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene071859.t1 MTR_2g435340 97.345 452 12 0 1 452 1 452 0.0 897
MS.gene071859.t1 MTR_2g435340 96.254 347 13 0 106 452 41 387 0.0 672
MS.gene071859.t1 MTR_4g120730 72.398 442 119 2 12 451 13 453 0.0 655
MS.gene071859.t1 MTR_7g110710 52.318 453 206 5 2 449 7 454 8.74e-168 480
MS.gene071859.t1 MTR_7g110710 52.318 453 206 5 2 449 7 454 9.13e-168 480
MS.gene071859.t1 MTR_1g086030 57.071 396 167 2 33 428 78 470 1.63e-162 468
MS.gene071859.t1 MTR_8g098840 50.761 394 193 1 33 425 40 433 5.53e-146 425
MS.gene071859.t1 MTR_7g088550 48.592 426 214 3 8 428 20 445 8.31e-145 422
MS.gene071859.t1 MTR_1g100050 47.319 429 224 2 2 428 14 442 1.67e-143 419
MS.gene071859.t1 MTR_1g100060 48.662 411 206 2 23 428 75 485 1.82e-140 412
MS.gene071859.t1 MTR_1g099850 49.874 397 198 1 33 428 51 447 2.84e-140 410
MS.gene071859.t1 MTR_3g449590 49.395 413 195 4 33 439 43 447 5.19e-140 409
MS.gene071859.t1 MTR_1g100040 48.139 403 208 1 28 429 47 449 2.26e-138 406
MS.gene071859.t1 MTR_7g078680 46.098 410 220 1 33 441 51 460 3.04e-135 398
MS.gene071859.t1 MTR_8g028725 43.750 464 249 3 1 452 16 479 2.37e-133 393
MS.gene071859.t1 MTR_5g090160 49.752 404 196 4 30 428 37 438 2.00e-129 382
MS.gene071859.t1 MTR_3g096330 48.786 412 207 2 32 443 71 478 2.43e-129 392
MS.gene071859.t1 MTR_4g124980 42.995 414 231 3 17 428 57 467 1.13e-123 369
MS.gene071859.t1 MTR_2g042740 44.417 403 220 3 28 428 49 449 4.17e-122 364
MS.gene071859.t1 MTR_1g086030 51.183 338 162 2 91 428 32 366 9.82e-121 358
MS.gene071859.t1 MTR_2g083670 52.273 220 90 1 24 243 27 231 1.01e-70 224
MS.gene071859.t1 MTR_6g048610 35.503 169 72 2 230 398 24 155 1.64e-27 107
MS.gene071859.t1 MTR_2g042750 41.722 151 77 3 33 181 117 258 1.02e-25 106
MS.gene071859.t1 MTR_8g065030 24.205 409 269 14 37 436 65 441 4.61e-21 95.9
MS.gene071859.t1 MTR_7g092470 26.744 430 232 17 30 428 41 418 2.33e-20 93.6
MS.gene071859.t1 MTR_6g005630 26.183 317 195 10 29 339 37 320 1.04e-19 91.3
MS.gene071859.t1 MTR_6g028080 24.939 413 253 15 33 428 27 399 1.18e-19 90.9
MS.gene071859.t1 MTR_5g043350 26.220 328 186 10 38 348 30 318 1.50e-19 90.5
MS.gene071859.t1 MTR_5g090140 34.426 183 77 6 250 428 1 144 1.39e-18 82.8
MS.gene071859.t1 MTR_5g033680 24.233 326 197 10 28 335 47 340 3.73e-18 87.0
MS.gene071859.t1 MTR_3g084380 24.731 372 227 10 39 398 23 353 5.83e-18 85.9
MS.gene071859.t1 MTR_1g052315 21.103 417 259 12 23 413 46 418 4.07e-16 80.5
MS.gene071859.t1 MTR_6g005630 25.316 316 176 10 29 339 37 297 5.45e-16 80.1
MS.gene071859.t1 MTR_5g034090 24.432 352 192 11 28 339 110 427 8.03e-16 80.1
MS.gene071859.t1 MTR_8g010080 24.919 309 185 9 39 339 33 302 2.51e-15 77.8
MS.gene071859.t1 MTR_8g010040 26.941 438 247 17 2 423 4 384 2.80e-15 77.8
MS.gene071859.t1 MTR_8g077320 28.718 195 126 4 150 339 110 296 2.91e-15 77.4
MS.gene071859.t1 MTR_3g054320 24.121 398 246 12 39 422 32 387 3.86e-15 77.4
MS.gene071859.t1 MTR_4g084050 24.252 301 187 10 38 330 24 291 1.19e-14 75.9
MS.gene071859.t1 MTR_7g091840 23.824 319 186 9 39 339 67 346 2.13e-14 75.1
MS.gene071859.t1 MTR_1g086390 26.019 319 191 10 37 339 81 370 3.64e-14 74.7
MS.gene071859.t1 MTR_1g012390 25.307 407 246 15 35 424 26 391 3.91e-14 74.3
MS.gene071859.t1 MTR_2g060350 23.273 275 187 4 150 413 168 429 5.61e-14 73.9
MS.gene071859.t1 MTR_4g094715 27.492 331 185 14 31 339 55 352 6.43e-14 73.9
MS.gene071859.t1 MTR_7g035415 25.588 340 195 11 23 347 26 322 1.89e-13 71.6
MS.gene071859.t1 MTR_7g091850 23.691 401 237 12 37 413 76 431 4.56e-13 71.2
MS.gene071859.t1 MTR_3g084360 23.961 409 250 14 36 425 23 389 5.38e-13 70.9
MS.gene071859.t1 MTR_8g008765 22.946 353 200 11 39 375 33 329 1.20e-12 69.3
MS.gene071859.t1 MTR_8g010110 24.194 310 187 9 39 339 33 303 1.46e-12 69.3
MS.gene071859.t1 MTR_8g010090 23.885 314 183 10 39 339 33 303 2.00e-12 68.9
MS.gene071859.t1 MTR_2g049400 24.691 405 249 14 36 425 27 390 1.04e-11 66.6
MS.gene071859.t1 MTR_2g103410 28.141 199 119 5 150 339 157 340 2.27e-11 65.9
MS.gene071859.t1 MTR_8g010070 25.962 312 180 12 39 339 33 304 2.91e-11 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene071859.t1 AT3G16850 63.719 441 153 3 1 440 3 437 0.0 579
MS.gene071859.t1 AT3G06770 63.409 440 155 3 1 434 1 440 0.0 562
MS.gene071859.t1 AT3G06770 63.470 438 154 3 3 434 4 441 0.0 560
MS.gene071859.t1 AT5G49215 65.375 413 141 2 18 428 24 436 0.0 549
MS.gene071859.t1 AT5G49215 65.375 413 141 2 18 428 22 434 0.0 549
MS.gene071859.t1 AT3G06770 64.865 370 130 0 65 434 2 371 7.18e-174 492
MS.gene071859.t1 AT3G06770 64.865 370 130 0 65 434 2 371 7.18e-174 492
MS.gene071859.t1 AT3G62110 53.596 431 197 3 1 428 6 436 9.72e-167 478
MS.gene071859.t1 AT3G62110 53.596 431 197 3 1 428 6 436 9.72e-167 478
MS.gene071859.t1 AT3G06770 60.411 341 135 0 94 434 13 353 5.78e-145 418
MS.gene071859.t1 AT3G48950 49.377 401 201 2 30 428 38 438 1.55e-144 421
MS.gene071859.t1 AT4G23500 50.378 397 196 1 33 428 64 460 9.69e-141 412
MS.gene071859.t1 AT4G23820 48.028 431 214 3 3 428 6 431 1.29e-140 410
MS.gene071859.t1 AT3G61490 44.700 434 234 2 1 428 4 437 1.93e-136 401
MS.gene071859.t1 AT3G61490 44.700 434 234 2 1 428 4 437 1.93e-136 401
MS.gene071859.t1 AT3G61490 44.700 434 234 2 1 428 4 437 1.93e-136 401
MS.gene071859.t1 AT3G61490 44.700 434 234 2 1 428 19 452 4.44e-136 400
MS.gene071859.t1 AT3G61490 44.700 434 234 2 1 428 117 550 4.57e-135 401
MS.gene071859.t1 AT4G33440 47.529 425 220 2 18 440 51 474 7.03e-132 389
MS.gene071859.t1 AT2G23900 46.377 414 218 3 30 441 49 460 2.82e-131 387
MS.gene071859.t1 AT5G41870 46.420 433 220 5 1 428 10 435 2.78e-130 384
MS.gene071859.t1 AT3G42950 41.191 403 233 3 28 428 59 459 1.72e-115 347
MS.gene071859.t1 AT1G19170 42.169 415 235 4 17 428 69 481 2.79e-113 343
MS.gene071859.t1 AT3G15720 27.470 415 251 16 35 436 22 399 2.30e-24 105
MS.gene071859.t1 AT3G15720 27.470 415 251 16 35 436 21 398 2.46e-24 105
MS.gene071859.t1 AT3G15720 27.470 415 248 17 35 436 21 395 1.54e-22 100
MS.gene071859.t1 AT1G23460 24.810 395 255 13 37 421 43 405 2.43e-21 96.3
MS.gene071859.t1 AT1G23460 24.810 395 255 13 37 421 67 429 3.11e-21 96.3
MS.gene071859.t1 AT1G70500 25.445 393 245 13 37 416 67 424 1.12e-20 94.7
MS.gene071859.t1 AT3G15720 27.922 308 191 10 140 436 48 335 1.86e-20 93.2
MS.gene071859.t1 AT2G41850 28.302 318 183 10 34 339 65 349 5.37e-20 92.4
MS.gene071859.t1 AT3G57510 25.195 385 235 12 36 404 67 414 1.79e-19 90.9
MS.gene071859.t1 AT3G57790 25.428 409 221 13 36 408 34 394 8.48e-18 86.3
MS.gene071859.t1 AT3G57790 25.428 409 221 13 36 408 34 394 8.48e-18 86.3
MS.gene071859.t1 AT5G17200 25.987 304 178 8 37 332 39 303 1.25e-16 82.0
MS.gene071859.t1 AT3G26610 24.119 369 220 12 1 339 12 350 1.84e-16 82.0
MS.gene071859.t1 AT5G14650 24.601 313 193 10 36 339 50 328 1.89e-16 81.6
MS.gene071859.t1 AT1G48100 28.037 321 176 14 39 339 82 367 1.02e-15 79.7
MS.gene071859.t1 AT3G07970 25.993 277 175 7 150 413 167 426 3.66e-15 77.8
MS.gene071859.t1 AT1G56710 25.625 320 197 9 34 339 45 337 8.99e-15 76.6
MS.gene071859.t1 AT2G15470 25.610 410 254 15 33 425 27 402 9.04e-14 73.2
MS.gene071859.t1 AT2G43860 24.939 409 253 16 32 425 28 397 9.42e-14 73.2
MS.gene071859.t1 AT1G60590 23.824 340 190 11 38 339 124 432 1.54e-13 73.2
MS.gene071859.t1 AT1G02790 26.074 349 212 11 34 375 49 358 2.30e-13 72.0
MS.gene071859.t1 AT1G02460 25.857 321 190 12 38 339 90 381 2.73e-13 72.0
MS.gene071859.t1 AT2G26620 25.735 408 254 16 33 425 27 400 2.84e-13 71.6
MS.gene071859.t1 AT3G59850 23.573 403 256 13 35 424 21 384 5.61e-13 70.9
MS.gene071859.t1 AT2G43870 24.603 378 234 11 35 399 19 358 8.24e-13 70.1
MS.gene071859.t1 AT3G59850 23.192 401 260 11 35 424 65 428 1.08e-12 70.1
MS.gene071859.t1 AT2G15460 25.686 401 248 14 33 417 27 393 1.08e-12 70.1
MS.gene071859.t1 AT1G80170 24.213 413 258 14 33 425 46 423 2.06e-12 69.3
MS.gene071859.t1 AT2G15450 25.245 408 255 14 33 424 27 400 2.49e-12 68.9
MS.gene071859.t1 AT1G17150 24.331 411 251 13 33 424 29 398 2.75e-12 68.6
MS.gene071859.t1 AT5G14650 26.009 223 139 7 36 253 50 251 4.11e-12 67.4
MS.gene071859.t1 AT2G43880 23.383 402 258 15 36 425 30 393 7.07e-12 67.4
MS.gene071859.t1 AT1G43080 23.133 415 262 16 33 427 27 404 1.51e-11 66.2
MS.gene071859.t1 AT2G40310 24.455 413 255 16 33 425 27 402 2.57e-11 65.5
MS.gene071859.t1 AT4G13760 24.578 415 252 16 33 425 27 402 8.67e-11 63.9

Find 105 sgRNAs with CRISPR-Local

Find 213 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTCCGTTTCTTCAAGTAAA+TGG 0.088505 2.2:+53488755 MS.gene071859:CDS
AGCAATGCCGTGAATGTTTA+TGG 0.195198 2.2:+53486416 MS.gene071859:CDS
ATGTCGTTTGAATCCTAAAT+TGG 0.263811 2.2:+53486451 MS.gene071859:CDS
ATTGAAGCTTTACCTTCTTA+TGG 0.264280 2.2:+53487255 MS.gene071859:CDS
GGTACTATACCGGCAGTATA+AGG 0.271970 2.2:-53487766 None:intergenic
ATTTCAGCTAAAGGTAATTC+TGG 0.272185 2.2:+53488568 MS.gene071859:CDS
TGCAAAAGTGGAAGTGCTTT+TGG 0.294030 2.2:-53488616 None:intergenic
ATTATTTACCTGCAATAAAC+AGG 0.300438 2.2:-53487616 None:intergenic
AGTGTCTCTATATTGGAATT+TGG 0.306110 2.2:+53486488 MS.gene071859:CDS
CCACCAGGAAACTGGCTTAC+TGG 0.322724 2.2:+53486611 MS.gene071859:CDS
TATGTAACCACCTCTTCCTT+TGG 0.328460 2.2:-53488497 None:intergenic
TGAGATAGAAAATTGCATTC+TGG 0.331713 2.2:-53486550 None:intergenic
TGTGGCCTAGGATCCAATTT+AGG 0.332220 2.2:-53486464 None:intergenic
GATCAGTACGGCATTAAATA+TGG 0.336655 2.2:+53488316 MS.gene071859:CDS
GCCACACAGTGTCTCTATAT+TGG 0.338491 2.2:+53486481 MS.gene071859:CDS
TTGGAAGATGTGGTCATAAC+AGG 0.339246 2.2:+53487333 MS.gene071859:CDS
CCGGCAGTATAAGGGGATTC+TGG 0.339380 2.2:-53487757 None:intergenic
GTTCATCTTCGTGCATTTAC+CGG 0.342309 2.2:+53488370 MS.gene071859:CDS
GGAAAGAATATCACAATTGC+TGG 0.350318 2.2:+53488664 MS.gene071859:CDS
TCTCAAGTCATTTGCTGATA+AGG 0.351226 2.2:+53486568 MS.gene071859:CDS
ACCGGCTCTGCTATTTCATT+TGG 0.351598 2.2:+53488388 MS.gene071859:CDS
AAATGTTAACATGCTCTATC+AGG 0.358232 2.2:-53488441 None:intergenic
GGCAATAATGGAACCATTGA+TGG 0.360386 2.2:+53487454 MS.gene071859:intron
GTACTATACCGGCAGTATAA+GGG 0.366594 2.2:-53487765 None:intergenic
TTTGGATCAAATTTGTCATC+AGG 0.370603 2.2:-53488598 None:intergenic
ATGGAATGGTTTGGTGGGAC+TGG 0.373444 2.2:+53487479 MS.gene071859:CDS
GATGAGGACGACTGTGGTTA+AGG 0.378902 2.2:-53487516 None:intergenic
TCTATGTGCCACCAGGAAAC+TGG 0.379700 2.2:+53486603 MS.gene071859:CDS
TGCTTTATCACACATAGAAA+TGG 0.384138 2.2:+53488528 MS.gene071859:CDS
TTCCGTTTCTTCAAGTAAAT+GGG 0.384317 2.2:+53488756 MS.gene071859:CDS
TATACAGCAATTTCAGCTAA+AGG 0.395099 2.2:+53488559 MS.gene071859:CDS
TGCTGTCATTATTGGATCTC+AGG 0.395803 2.2:+53486679 MS.gene071859:CDS
CTTATATGTACTTTCTCACT+TGG 0.401622 2.2:-53488343 None:intergenic
CCAAGTTCTTGACAAGGTTT+AGG 0.403560 2.2:-53488817 None:intergenic
ATAACATTGAAGGATATAAC+TGG 0.408428 2.2:+53488643 MS.gene071859:CDS
GCATCCACCCTGTTTATTGC+AGG 0.409422 2.2:+53487608 MS.gene071859:CDS
AACCATTCCATTGCCATCAA+TGG 0.414602 2.2:-53487467 None:intergenic
AGATACAAGAGCTTGATAAA+TGG 0.415928 2.2:+53487303 MS.gene071859:CDS
CTCAAGTCATTTGCTGATAA+GGG 0.419974 2.2:+53486569 MS.gene071859:CDS
CCTAAACCTTGTCAAGAACT+TGG 0.423904 2.2:+53488817 MS.gene071859:CDS
TGAATCCTAAATTGGATCCT+AGG 0.429800 2.2:+53486459 MS.gene071859:CDS
TTCCCATTTACTTGAAGAAA+CGG 0.430980 2.2:-53488758 None:intergenic
ATATTGGAATTTGGTGCAGT+TGG 0.436498 2.2:+53486497 MS.gene071859:CDS
TTTGCATCACATAACATTGA+AGG 0.443614 2.2:+53488633 MS.gene071859:CDS
CTACCAGTAAGCCAGTTTCC+TGG 0.448344 2.2:-53486614 None:intergenic
ATAGAGACACTGTGTGGCCT+AGG 0.453912 2.2:-53486476 None:intergenic
GACGCAATTTCTCTCAAAAG+TGG 0.454842 2.2:+53488289 MS.gene071859:CDS
TTGTTTAGGATCCATCTCAT+TGG 0.466913 2.2:+53487226 MS.gene071859:intron
TTATGACCACATCTTCCAAC+TGG 0.467822 2.2:-53487329 None:intergenic
ATGAGGACGACTGTGGTTAA+GGG 0.472010 2.2:-53487515 None:intergenic
GAAGATTGTTATGTTTCCAT+TGG 0.475742 2.2:+53488262 MS.gene071859:CDS
GTGAGAAAGTACATATAAGA+AGG 0.477334 2.2:+53488347 MS.gene071859:CDS
ATGGGAATGTTCAAATGTCT+CGG 0.480367 2.2:+53488774 MS.gene071859:CDS
TCATTTGGTACTGAAATGTC+TGG 0.487250 2.2:+53488403 MS.gene071859:CDS
AAGCTCTTGTATCTTCCGTC+AGG 0.495493 2.2:-53487294 None:intergenic
TAGACAAGGTTATATTTGAA+AGG 0.499579 2.2:-53488729 None:intergenic
AACATCTCCATAAACATTCA+CGG 0.500990 2.2:-53486423 None:intergenic
CAATTTCTCTCAAAAGTGGA+TGG 0.507018 2.2:+53488293 MS.gene071859:CDS
GATGACGAAGAAGAATTTGA+AGG 0.513232 2.2:-53488844 None:intergenic
TGGGAATGTTCAAATGTCTC+GGG 0.517276 2.2:+53488775 MS.gene071859:CDS
ACCAAATGAAATAGCAGAGC+CGG 0.517644 2.2:-53488389 None:intergenic
AATGGAACCATTGATGGCAA+TGG 0.521099 2.2:+53487460 MS.gene071859:CDS
TCTTTCCCTCGGCCATAAGA+AGG 0.523156 2.2:-53487267 None:intergenic
AATGTTATGTGATGCAAAAG+TGG 0.524613 2.2:-53488628 None:intergenic
TCAATAACATTCCAATGAGA+TGG 0.528619 2.2:-53487237 None:intergenic
CCAGAATCCCCTTATACTGC+CGG 0.534389 2.2:+53487757 MS.gene071859:CDS
GCTTTACCTTCTTATGGCCG+AGG 0.543091 2.2:+53487261 MS.gene071859:CDS
CTTGATAAATGGTAACCAGT+TGG 0.546984 2.2:+53487314 MS.gene071859:CDS
TGGTAACCAGTTGGAAGATG+TGG 0.548355 2.2:+53487323 MS.gene071859:CDS
CGTCAGGAACATCTTTCCCT+CGG 0.553651 2.2:-53487278 None:intergenic
ACAGGGTGGATGCTATATGC+TGG 0.557217 2.2:-53487598 None:intergenic
AGAAATTGCGTCAAAACCAA+TGG 0.560982 2.2:-53488278 None:intergenic
TTCAGAACAACCAAAGGAAG+AGG 0.562716 2.2:+53488487 MS.gene071859:CDS
TTACTAACCTGGTACTATAC+CGG 0.564112 2.2:-53487776 None:intergenic
ATGGCAATGGAATGGTTTGG+TGG 0.564149 2.2:+53487473 MS.gene071859:CDS
GAATTTGGTGCAGTTGGAGA+TGG 0.565909 2.2:+53486503 MS.gene071859:CDS
TGGCAATGGAATGGTTTGGT+GGG 0.576997 2.2:+53487474 MS.gene071859:CDS
TTGATGGCAATGGAATGGTT+TGG 0.583352 2.2:+53487470 MS.gene071859:CDS
ATGACGAAGAAGAATTTGAA+GGG 0.583947 2.2:-53488843 None:intergenic
TACTATACCGGCAGTATAAG+GGG 0.586797 2.2:-53487764 None:intergenic
TGAGAAAGTACATATAAGAA+GGG 0.590695 2.2:+53488348 MS.gene071859:CDS
AGAAACGGAATTTGTAGACA+AGG 0.591928 2.2:-53488743 None:intergenic
CTTTACCTTCTTATGGCCGA+GGG 0.592122 2.2:+53487262 MS.gene071859:CDS
TGAAAGGCATATGTTTGTGA+AGG 0.592606 2.2:-53488713 None:intergenic
GATATAATTTCAACGAGATG+AGG 0.595103 2.2:-53487532 None:intergenic
GCTCAGCTCTATGTGCCACC+AGG 0.597340 2.2:+53486596 MS.gene071859:CDS
TATACTGCCGGTATAGTACC+AGG 0.604603 2.2:+53487769 MS.gene071859:CDS
TGTTACTACCTCATGAGTCT+CGG 0.607492 2.2:+53488868 MS.gene071859:CDS
AATTTCTCTCAAAAGTGGAT+GGG 0.608979 2.2:+53488294 MS.gene071859:CDS
AACCATTGATGGCAATGGAA+TGG 0.612706 2.2:+53487465 MS.gene071859:CDS
AAAAGTGGATGGGATCAGTA+CGG 0.626304 2.2:+53488304 MS.gene071859:CDS
TTTGGTACTGAAATGTCTGG+TGG 0.627310 2.2:+53488406 MS.gene071859:CDS
GAAAGGCATATGTTTGTGAA+GGG 0.628625 2.2:-53488712 None:intergenic
ACAATTGCTGGAATCTTGAG+TGG 0.630331 2.2:+53488676 MS.gene071859:CDS
GGGTTACCAAGTTCTTGACA+AGG 0.644208 2.2:-53488823 None:intergenic
CGAGGGAAAGATGTTCCTGA+CGG 0.645144 2.2:+53487279 MS.gene071859:CDS
ATTGAGTTCAGAACAACCAA+AGG 0.651524 2.2:+53488481 MS.gene071859:CDS
AGAACAACCAAAGGAAGAGG+TGG 0.657978 2.2:+53488490 MS.gene071859:CDS
AAGTCATTTGCTGATAAGGG+TGG 0.660065 2.2:+53486572 MS.gene071859:CDS
CAACGAGATGAGGACGACTG+TGG 0.660908 2.2:-53487522 None:intergenic
TTATTTACCTGCAATAAACA+GGG 0.669198 2.2:-53487615 None:intergenic
TTTACCTGCAATAAACAGGG+TGG 0.674542 2.2:-53487612 None:intergenic
GCAGTATAAGGGGATTCTGG+AGG 0.680237 2.2:-53487754 None:intergenic
TCCAATATAGAGACACTGTG+TGG 0.732684 2.2:-53486482 None:intergenic
CCAGTAAGCCAGTTTCCTGG+TGG 0.733343 2.2:-53486611 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACTTCAACAAAAATATTATA+AGG - chr2.2:53486022-53486041 None:intergenic 15.0%
!! ATGATATAATTGAATATACT+CGG - chr2.2:53488067-53488086 None:intergenic 15.0%
!!! CATTTTTCAAATAATTACTA+GGG + chr2.2:53488084-53488103 MS.gene071859:intron 15.0%
!!! TCATTTTTCAAATAATTACT+AGG + chr2.2:53488083-53488102 MS.gene071859:intron 15.0%
!!! TTTTTCATTTAATTTTTACC+AGG + chr2.2:53487433-53487452 MS.gene071859:intron 15.0%
!! ATCAATATATCAGATCAAAA+TGG - chr2.2:53485926-53485945 None:intergenic 20.0%
!! ATCTTTGAATGTATTGAAAT+TGG + chr2.2:53486790-53486809 MS.gene071859:intron 20.0%
!! TCATACATAAAATTTGAACA+TGG - chr2.2:53485823-53485842 None:intergenic 20.0%
!! TCTTTGAATGTATTGAAATT+GGG + chr2.2:53486791-53486810 MS.gene071859:intron 20.0%
!! TTACCATCTCAAATAATTAT+CGG - chr2.2:53487969-53487988 None:intergenic 20.0%
!! TTCTTCTTTGATACTTAATT+TGG + chr2.2:53487649-53487668 MS.gene071859:intron 20.0%
!!! AATTATATCATCTGATTTTG+TGG + chr2.2:53487546-53487565 MS.gene071859:CDS 20.0%
!!! TGCTTTGTTTTTCATTTTAA+GGG + chr2.2:53485725-53485744 MS.gene071859:intron 20.0%
!!! TTCTATGTTGTTTTAAATCT+GGG + chr2.2:53486321-53486340 MS.gene071859:intron 20.0%
!!! TTGCTTTGTTTTTCATTTTA+AGG + chr2.2:53485724-53485743 MS.gene071859:intron 20.0%
!!! TTTCTATGTTGTTTTAAATC+TGG + chr2.2:53486320-53486339 MS.gene071859:intron 20.0%
!!! TTTTTGTGTTTTGTTCATAT+GGG + chr2.2:53485797-53485816 MS.gene071859:intron 20.0%
! AATTGTGTATTGTTCCTATT+TGG + chr2.2:53487036-53487055 MS.gene071859:intron 25.0%
! AATTTGGCAGACAAAATTAA+GGG + chr2.2:53487665-53487684 MS.gene071859:intron 25.0%
! ACTAACAAAATTCTTACTCA+GGG - chr2.2:53486824-53486843 None:intergenic 25.0%
! ATAACATTGAAGGATATAAC+TGG + chr2.2:53488643-53488662 MS.gene071859:CDS 25.0%
! ATACCGATAATTATTTGAGA+TGG + chr2.2:53487963-53487982 MS.gene071859:intron 25.0%
! ATTATTTACCTGCAATAAAC+AGG - chr2.2:53487619-53487638 None:intergenic 25.0%
! CTTTAATATGTTTACTAACC+TGG - chr2.2:53487790-53487809 None:intergenic 25.0%
! TAATTTGGCAGACAAAATTA+AGG + chr2.2:53487664-53487683 MS.gene071859:intron 25.0%
! TAGACAAGGTTATATTTGAA+AGG - chr2.2:53488732-53488751 None:intergenic 25.0%
! TTATTTACCTGCAATAAACA+GGG - chr2.2:53487618-53487637 None:intergenic 25.0%
!! AACATTTTCAACTCAAATAG+TGG + chr2.2:53488457-53488476 MS.gene071859:CDS 25.0%
!! CCATGAGTAAAAAATCATTT+TGG - chr2.2:53486979-53486998 None:intergenic 25.0%
!! TAATTTTTACCAGGCAATAA+TGG + chr2.2:53487442-53487461 MS.gene071859:intron 25.0%
!! TGAGAAAGTACATATAAGAA+GGG + chr2.2:53488348-53488367 MS.gene071859:CDS 25.0%
!!! ATGTATGAGATTTGTTCTTT+TGG + chr2.2:53485835-53485854 MS.gene071859:intron 25.0%
!!! CCAAAATGATTTTTTACTCA+TGG + chr2.2:53486976-53486995 MS.gene071859:intron 25.0%
!!! GTTTTTGTGTTTTGTTCATA+TGG + chr2.2:53485796-53485815 MS.gene071859:intron 25.0%
!!! GTTTTTTTGTTCCTTTTGTT+TGG + chr2.2:53485858-53485877 MS.gene071859:intron 25.0%
!!! TCTCTTCAAGTTTTGATTTT+TGG + chr2.2:53485748-53485767 MS.gene071859:intron 25.0%
!!! TGTATGAGATTTGTTCTTTT+GGG + chr2.2:53485836-53485855 MS.gene071859:intron 25.0%
!!! TTCAAGTTTTGATTTTTGGT+TGG + chr2.2:53485752-53485771 MS.gene071859:intron 25.0%
AAATGTTAACATGCTCTATC+AGG - chr2.2:53488444-53488463 None:intergenic 30.0%
AACATCTCCATAAACATTCA+CGG - chr2.2:53486426-53486445 None:intergenic 30.0%
AATCAAATTTCAGGAGTTAC+TGG - chr2.2:53486732-53486751 None:intergenic 30.0%
AATGCTTCATAGGTTATGTT+TGG + chr2.2:53486296-53486315 MS.gene071859:intron 30.0%
AATGTTATGTGATGCAAAAG+TGG - chr2.2:53488631-53488650 None:intergenic 30.0%
AATTCACTTCAAATTGACAG+AGG - chr2.2:53486179-53486198 None:intergenic 30.0%
AATTTCTCTCAAAAGTGGAT+GGG + chr2.2:53488294-53488313 MS.gene071859:CDS 30.0%
AGATACAAGAGCTTGATAAA+TGG + chr2.2:53487303-53487322 MS.gene071859:CDS 30.0%
AGTGTCTCTATATTGGAATT+TGG + chr2.2:53486488-53486507 MS.gene071859:CDS 30.0%
ATGACGAAGAAGAATTTGAA+GGG - chr2.2:53488846-53488865 None:intergenic 30.0%
ATTCCGTTTCTTCAAGTAAA+TGG + chr2.2:53488755-53488774 MS.gene071859:CDS 30.0%
ATTGAAGCTTTACCTTCTTA+TGG + chr2.2:53487255-53487274 MS.gene071859:CDS 30.0%
ATTTCAGCTAAAGGTAATTC+TGG + chr2.2:53488568-53488587 MS.gene071859:CDS 30.0%
CACTAACAAAATTCTTACTC+AGG - chr2.2:53486825-53486844 None:intergenic 30.0%
CTTAAACTAGTTCCAAATCT+GGG + chr2.2:53485695-53485714 MS.gene071859:intron 30.0%
CTTATATGTACTTTCTCACT+TGG - chr2.2:53488346-53488365 None:intergenic 30.0%
GAAGATTGTTATGTTTCCAT+TGG + chr2.2:53488262-53488281 MS.gene071859:CDS 30.0%
GACGAAACTAATCAAATTTC+AGG - chr2.2:53486741-53486760 None:intergenic 30.0%
GATAGAAGCAAGAACAAATT+TGG - chr2.2:53487374-53487393 None:intergenic 30.0%
GATATAATTTCAACGAGATG+AGG - chr2.2:53487535-53487554 None:intergenic 30.0%
GCAAAAACAATGAAAGAACT+AGG - chr2.2:53486088-53486107 None:intergenic 30.0%
GCTATATTATTGTAGCGTAT+CGG + chr2.2:53487092-53487111 MS.gene071859:intron 30.0%
GTAAGCTATTGACATTTGAA+AGG - chr2.2:53488166-53488185 None:intergenic 30.0%
TAAACCAATTAATCACCAGA+AGG - chr2.2:53486354-53486373 None:intergenic 30.0%
TACAAAATGTTGTCAAATGG+CGG + chr2.2:53487059-53487078 MS.gene071859:intron 30.0%
TATACAGCAATTTCAGCTAA+AGG + chr2.2:53488559-53488578 MS.gene071859:CDS 30.0%
TCAATAACATTCCAATGAGA+TGG - chr2.2:53487240-53487259 None:intergenic 30.0%
TGAAATTTCATAAACATCCC+TGG - chr2.2:53486913-53486932 None:intergenic 30.0%
TGAGATAGAAAATTGCATTC+TGG - chr2.2:53486553-53486572 None:intergenic 30.0%
TGATTAACAACAATCACCAT+TGG - chr2.2:53485894-53485913 None:intergenic 30.0%
TGCTTTATCACACATAGAAA+TGG + chr2.2:53488528-53488547 MS.gene071859:CDS 30.0%
TGGTACAAAATGTTGTCAAA+TGG + chr2.2:53487056-53487075 MS.gene071859:intron 30.0%
TTCATGTGAAACCAATTTGA+GGG + chr2.2:53487183-53487202 MS.gene071859:intron 30.0%
TTCCCATTTACTTGAAGAAA+CGG - chr2.2:53488761-53488780 None:intergenic 30.0%
TTCCGTTTCTTCAAGTAAAT+GGG + chr2.2:53488756-53488775 MS.gene071859:CDS 30.0%
TTTCATGTGAAACCAATTTG+AGG + chr2.2:53487182-53487201 MS.gene071859:intron 30.0%
TTTGCATCACATAACATTGA+AGG + chr2.2:53488633-53488652 MS.gene071859:CDS 30.0%
TTTGGATCAAATTTGTCATC+AGG - chr2.2:53488601-53488620 None:intergenic 30.0%
! AAAATCATTTTGGACCTTGA+AGG - chr2.2:53486969-53486988 None:intergenic 30.0%
! ATGTCGTTTGAATCCTAAAT+TGG + chr2.2:53486451-53486470 MS.gene071859:CDS 30.0%
! CTGAGTAAGAATTTTGTTAG+TGG + chr2.2:53486823-53486842 MS.gene071859:intron 30.0%
! GACAACATTTTGTACCAAAT+AGG - chr2.2:53487053-53487072 None:intergenic 30.0%
! GTGAGAAAGTACATATAAGA+AGG + chr2.2:53488347-53488366 MS.gene071859:CDS 30.0%
! GTTTAAGGTGAAAACTCAAA+AGG - chr2.2:53485682-53485701 None:intergenic 30.0%
! TCTTGCTTCTATCAAGTTTT+TGG + chr2.2:53487381-53487400 MS.gene071859:intron 30.0%
! TGTATGTGATAATGCTTCAT+AGG + chr2.2:53486286-53486305 MS.gene071859:intron 30.0%
AAAGGTTGTCTATGAAGTAC+TGG - chr2.2:53488148-53488167 None:intergenic 35.0%
AACCAATTTGAGGGTAATTG+AGG + chr2.2:53487192-53487211 MS.gene071859:intron 35.0%
AAGCAATATAGTCCCAGATT+TGG - chr2.2:53485710-53485729 None:intergenic 35.0%
ACGAGGATCAAGCTTATTAA+GGG + chr2.2:53486878-53486897 MS.gene071859:intron 35.0%
AGAAACGGAATTTGTAGACA+AGG - chr2.2:53488746-53488765 None:intergenic 35.0%
AGAAATTGCGTCAAAACCAA+TGG - chr2.2:53488281-53488300 None:intergenic 35.0%
ATGGGAATGTTCAAATGTCT+CGG + chr2.2:53488774-53488793 MS.gene071859:CDS 35.0%
ATTGAGTTCAGAACAACCAA+AGG + chr2.2:53488481-53488500 MS.gene071859:CDS 35.0%
CAATTTCTCTCAAAAGTGGA+TGG + chr2.2:53488293-53488312 MS.gene071859:CDS 35.0%
CCTTAAACTAGTTCCAAATC+TGG + chr2.2:53485694-53485713 MS.gene071859:intron 35.0%
CTGGTAAAAAATGAAGCAAG+AGG - chr2.2:53486713-53486732 None:intergenic 35.0%
CTGTTATCCTCATTGTGAAA+TGG + chr2.2:53487818-53487837 MS.gene071859:intron 35.0%
CTTCAGATTGAATGAGTGTT+CGG + chr2.2:53487865-53487884 MS.gene071859:intron 35.0%
CTTGATAAATGGTAACCAGT+TGG + chr2.2:53487314-53487333 MS.gene071859:CDS 35.0%
CTTTCCTTCTGGTGATTAAT+TGG + chr2.2:53486347-53486366 MS.gene071859:intron 35.0%
GAAGTGATTCTCTCATTGAT+TGG + chr2.2:53486037-53486056 MS.gene071859:intron 35.0%
GATCAGTACGGCATTAAATA+TGG + chr2.2:53488316-53488335 MS.gene071859:CDS 35.0%
GATGACGAAGAAGAATTTGA+AGG - chr2.2:53488847-53488866 None:intergenic 35.0%
GCATATACATGCCAAACAAA+AGG - chr2.2:53485872-53485891 None:intergenic 35.0%
GGAAAGAATATCACAATTGC+TGG + chr2.2:53488664-53488683 MS.gene071859:CDS 35.0%
TGAATCCTAAATTGGATCCT+AGG + chr2.2:53486459-53486478 MS.gene071859:CDS 35.0%
TTACTAACCTGGTACTATAC+CGG - chr2.2:53487779-53487798 None:intergenic 35.0%
TTGAATGTAAGCACACATGT+TGG + chr2.2:53487994-53488013 MS.gene071859:intron 35.0%
! AGCACCTTTTTCCAAAAAGA+GGG - chr2.2:53486663-53486682 None:intergenic 35.0%
! CCAGATTTGGAACTAGTTTA+AGG - chr2.2:53485697-53485716 None:intergenic 35.0%
! CTCAAGTCATTTGCTGATAA+GGG + chr2.2:53486569-53486588 MS.gene071859:CDS 35.0%
! GAAAGGCATATGTTTGTGAA+GGG - chr2.2:53488715-53488734 None:intergenic 35.0%
! GAATTTTGTTAGTGGTTTCG+TGG + chr2.2:53486831-53486850 MS.gene071859:intron 35.0%
! TCATTTGGTACTGAAATGTC+TGG + chr2.2:53488403-53488422 MS.gene071859:CDS 35.0%
! TCTCAAGTCATTTGCTGATA+AGG + chr2.2:53486568-53486587 MS.gene071859:CDS 35.0%
! TGAAAGGCATATGTTTGTGA+AGG - chr2.2:53488716-53488735 None:intergenic 35.0%
! TGGTTTATGTGACCTTTTTC+AGG + chr2.2:53486367-53486386 MS.gene071859:intron 35.0%
! TTGTTTAGGATCCATCTCAT+TGG + chr2.2:53487226-53487245 MS.gene071859:intron 35.0%
!! AGGAGTGGTTTTTCTTGTTT+AGG + chr2.2:53487212-53487231 MS.gene071859:intron 35.0%
!! ATATTGGAATTTGGTGCAGT+TGG + chr2.2:53486497-53486516 MS.gene071859:CDS 35.0%
!! GAAAAAGGTGCTGTCATTAT+TGG + chr2.2:53486671-53486690 MS.gene071859:CDS 35.0%
!!! TTTATGTGACCTTTTTCAGG+TGG + chr2.2:53486370-53486389 MS.gene071859:intron 35.0%
!!! TTTCGTCTTTTTTATGTGCG+AGG + chr2.2:53486754-53486773 MS.gene071859:intron 35.0%
AAAAGTGGATGGGATCAGTA+CGG + chr2.2:53488304-53488323 MS.gene071859:CDS 40.0%
AACCATTCCATTGCCATCAA+TGG - chr2.2:53487470-53487489 None:intergenic 40.0%
ACAATTGCTGGAATCTTGAG+TGG + chr2.2:53488676-53488695 MS.gene071859:CDS 40.0%
ACCAAATGAAATAGCAGAGC+CGG - chr2.2:53488392-53488411 None:intergenic 40.0%
AGCAATGCCGTGAATGTTTA+TGG + chr2.2:53486416-53486435 MS.gene071859:CDS 40.0%
AGTTGGACACAAGCATGTAT+TGG - chr2.2:53487894-53487913 None:intergenic 40.0%
ATTTGAGGGTAATTGAGGAG+TGG + chr2.2:53487197-53487216 MS.gene071859:intron 40.0%
CACGAGGATCAAGCTTATTA+AGG + chr2.2:53486877-53486896 MS.gene071859:intron 40.0%
CCAACTATGAGGCAAAAACA+CGG + chr2.2:53487908-53487927 MS.gene071859:intron 40.0%
CCAAGTTCTTGACAAGGTTT+AGG - chr2.2:53488820-53488839 None:intergenic 40.0%
CCTAAACCTTGTCAAGAACT+TGG + chr2.2:53488817-53488836 MS.gene071859:CDS 40.0%
CTCCTCAATTACCCTCAAAT+TGG - chr2.2:53487197-53487216 None:intergenic 40.0%
CTTGTAAGTACGTTTCACTC+TGG + chr2.2:53485621-53485640 MS.gene071859:intron 40.0%
GACGCAATTTCTCTCAAAAG+TGG + chr2.2:53488289-53488308 MS.gene071859:CDS 40.0%
GTACTATACCGGCAGTATAA+GGG - chr2.2:53487768-53487787 None:intergenic 40.0%
GTCAATAGCTTACCAAACCT+TGG + chr2.2:53488173-53488192 MS.gene071859:intron 40.0%
GTTCATCTTCGTGCATTTAC+CGG + chr2.2:53488370-53488389 MS.gene071859:CDS 40.0%
GTTTGGCATGTATATGCCAA+TGG + chr2.2:53485875-53485894 MS.gene071859:intron 40.0%
TACTATACCGGCAGTATAAG+GGG - chr2.2:53487767-53487786 None:intergenic 40.0%
TATGTAACCACCTCTTCCTT+TGG - chr2.2:53488500-53488519 None:intergenic 40.0%
TCAATGGTTCCATTATTGCC+TGG - chr2.2:53487454-53487473 None:intergenic 40.0%
TCCAATATAGAGACACTGTG+TGG - chr2.2:53486485-53486504 None:intergenic 40.0%
TGACTAACAAAAGAGACACG+AGG + chr2.2:53486861-53486880 MS.gene071859:intron 40.0%
TGCTGTCATTATTGGATCTC+AGG + chr2.2:53486679-53486698 MS.gene071859:CDS 40.0%
TGGGAATGTTCAAATGTCTC+GGG + chr2.2:53488775-53488794 MS.gene071859:CDS 40.0%
TGTTACTACCTCATGAGTCT+CGG + chr2.2:53488868-53488887 MS.gene071859:CDS 40.0%
TTATGACCACATCTTCCAAC+TGG - chr2.2:53487332-53487351 None:intergenic 40.0%
TTCAGAACAACCAAAGGAAG+AGG + chr2.2:53488487-53488506 MS.gene071859:CDS 40.0%
TTGGAAGATGTGGTCATAAC+AGG + chr2.2:53487333-53487352 MS.gene071859:CDS 40.0%
TTTACCTGCAATAAACAGGG+TGG - chr2.2:53487615-53487634 None:intergenic 40.0%
TTTCCAAAAAGAGGGTGAGA+TGG - chr2.2:53486655-53486674 None:intergenic 40.0%
! AACCATTGATGGCAATGGAA+TGG + chr2.2:53487465-53487484 MS.gene071859:CDS 40.0%
! AAGTCATTTGCTGATAAGGG+TGG + chr2.2:53486572-53486591 MS.gene071859:CDS 40.0%
! AATCTGGGATACTTTCCTTC+TGG + chr2.2:53486336-53486355 MS.gene071859:intron 40.0%
! AATGGAACCATTGATGGCAA+TGG + chr2.2:53487460-53487479 MS.gene071859:CDS 40.0%
! CAGCACCTTTTTCCAAAAAG+AGG - chr2.2:53486664-53486683 None:intergenic 40.0%
! GAAGTGTCAATGCTACATAG+AGG - chr2.2:53487849-53487868 None:intergenic 40.0%
! GGCAATAATGGAACCATTGA+TGG + chr2.2:53487454-53487473 MS.gene071859:intron 40.0%
! GTGTCGATGTTAGTCACTTA+TGG - chr2.2:53488040-53488059 None:intergenic 40.0%
! TTGATGGCAATGGAATGGTT+TGG + chr2.2:53487470-53487489 MS.gene071859:CDS 40.0%
! TTTGGTACTGAAATGTCTGG+TGG + chr2.2:53488406-53488425 MS.gene071859:CDS 40.0%
!! CTCACCCTCTTTTTGGAAAA+AGG + chr2.2:53486656-53486675 MS.gene071859:CDS 40.0%
!!! TATTTTGACCGAGACTCATG+AGG - chr2.2:53488879-53488898 None:intergenic 40.0%
!!! TGCAAAAGTGGAAGTGCTTT+TGG - chr2.2:53488619-53488638 None:intergenic 40.0%
AAGCTCTTGTATCTTCCGTC+AGG - chr2.2:53487297-53487316 None:intergenic 45.0%
AGAACAACCAAAGGAAGAGG+TGG + chr2.2:53488490-53488509 MS.gene071859:CDS 45.0%
AGAGCAATGCCACCTGAAAA+AGG - chr2.2:53486382-53486401 None:intergenic 45.0%
AGCTTACCAAACCTTGGTGA+GGG + chr2.2:53488179-53488198 MS.gene071859:intron 45.0%
ATGAGGACGACTGTGGTTAA+GGG - chr2.2:53487518-53487537 None:intergenic 45.0%
CATGCTTGTGTCCAACTATG+AGG + chr2.2:53487897-53487916 MS.gene071859:intron 45.0%
CTTTACCTTCTTATGGCCGA+GGG + chr2.2:53487262-53487281 MS.gene071859:CDS 45.0%
GCCACACAGTGTCTCTATAT+TGG + chr2.2:53486481-53486500 MS.gene071859:CDS 45.0%
GGGTTACCAAGTTCTTGACA+AGG - chr2.2:53488826-53488845 None:intergenic 45.0%
GGTACTATACCGGCAGTATA+AGG - chr2.2:53487769-53487788 None:intergenic 45.0%
TAGCTTACCAAACCTTGGTG+AGG + chr2.2:53488178-53488197 MS.gene071859:intron 45.0%
TATACTGCCGGTATAGTACC+AGG + chr2.2:53487769-53487788 MS.gene071859:CDS 45.0%
TGGTAACCAGTTGGAAGATG+TGG + chr2.2:53487323-53487342 MS.gene071859:CDS 45.0%
TGTGGCCTAGGATCCAATTT+AGG - chr2.2:53486467-53486486 None:intergenic 45.0%
! ATGGCAATGGAATGGTTTGG+TGG + chr2.2:53487473-53487492 MS.gene071859:CDS 45.0%
! ATTAAGGGCACACGTATCCA+GGG + chr2.2:53486893-53486912 MS.gene071859:intron 45.0%
! GGCATTGCCATTTCACAATG+AGG - chr2.2:53487828-53487847 None:intergenic 45.0%
! TAGCCATCTCACCCTCTTTT+TGG + chr2.2:53486649-53486668 MS.gene071859:CDS 45.0%
! TATTAAGGGCACACGTATCC+AGG + chr2.2:53486892-53486911 MS.gene071859:intron 45.0%
! TGTCTCGGGATTTTCAGACT+CGG + chr2.2:53488789-53488808 MS.gene071859:CDS 45.0%
!! ACCGGCTCTGCTATTTCATT+TGG + chr2.2:53488388-53488407 MS.gene071859:CDS 45.0%
!! CCGTGTTTTTGCCTCATAGT+TGG - chr2.2:53487911-53487930 None:intergenic 45.0%
!! GAATTTGGTGCAGTTGGAGA+TGG + chr2.2:53486503-53486522 MS.gene071859:CDS 45.0%
!! TGGCAATGGAATGGTTTGGT+GGG + chr2.2:53487474-53487493 MS.gene071859:CDS 45.0%
AAGTGACCCTCACCAAGGTT+TGG - chr2.2:53488188-53488207 None:intergenic 50.0%
ACAGGGTGGATGCTATATGC+TGG - chr2.2:53487601-53487620 None:intergenic 50.0%
ATAGAGACACTGTGTGGCCT+AGG - chr2.2:53486479-53486498 None:intergenic 50.0%
CCAGAATCCCCTTATACTGC+CGG + chr2.2:53487757-53487776 MS.gene071859:CDS 50.0%
CGAGGGAAAGATGTTCCTGA+CGG + chr2.2:53487279-53487298 MS.gene071859:CDS 50.0%
CGTCAGGAACATCTTTCCCT+CGG - chr2.2:53487281-53487300 None:intergenic 50.0%
CTACCAGTAAGCCAGTTTCC+TGG - chr2.2:53486617-53486636 None:intergenic 50.0%
GATGAGGACGACTGTGGTTA+AGG - chr2.2:53487519-53487538 None:intergenic 50.0%
GCAGTATAAGGGGATTCTGG+AGG - chr2.2:53487757-53487776 None:intergenic 50.0%
GCATCCACCCTGTTTATTGC+AGG + chr2.2:53487608-53487627 MS.gene071859:CDS 50.0%
GCTTTACCTTCTTATGGCCG+AGG + chr2.2:53487261-53487280 MS.gene071859:CDS 50.0%
TAGCTAAGTGACCCTCACCA+AGG - chr2.2:53488193-53488212 None:intergenic 50.0%
TCACATACACGTGATCAGCG+CGG - chr2.2:53486275-53486294 None:intergenic 50.0%
TCTATGTGCCACCAGGAAAC+TGG + chr2.2:53486603-53486622 MS.gene071859:CDS 50.0%
TCTTTCCCTCGGCCATAAGA+AGG - chr2.2:53487270-53487289 None:intergenic 50.0%
TGAGACGCTTGTCACCTTCA+AGG + chr2.2:53486952-53486971 MS.gene071859:intron 50.0%
!! ACGATACTGACTCTGACTCG+TGG + chr2.2:53487940-53487959 MS.gene071859:intron 50.0%
!! ATGGAATGGTTTGGTGGGAC+TGG + chr2.2:53487479-53487498 MS.gene071859:CDS 50.0%
CAACGAGATGAGGACGACTG+TGG - chr2.2:53487525-53487544 None:intergenic 55.0%
CCAGTAAGCCAGTTTCCTGG+TGG - chr2.2:53486614-53486633 None:intergenic 55.0%
CCGGCAGTATAAGGGGATTC+TGG - chr2.2:53487760-53487779 None:intergenic 55.0%
! CCACCAGGAAACTGGCTTAC+TGG + chr2.2:53486611-53486630 MS.gene071859:CDS 55.0%
GCTCAGCTCTATGTGCCACC+AGG + chr2.2:53486596-53486615 MS.gene071859:CDS 60.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 53485618 53488912 53485618 ID=MS.gene071859
chr2.2 mRNA 53485618 53488912 53485618 ID=MS.gene071859.t1;Parent=MS.gene071859
chr2.2 exon 53485618 53485623 53485618 ID=MS.gene071859.t1.exon1;Parent=MS.gene071859.t1
chr2.2 CDS 53485618 53485623 53485618 ID=cds.MS.gene071859.t1;Parent=MS.gene071859.t1
chr2.2 exon 53486389 53486700 53486389 ID=MS.gene071859.t1.exon2;Parent=MS.gene071859.t1
chr2.2 CDS 53486389 53486700 53486389 ID=cds.MS.gene071859.t1;Parent=MS.gene071859.t1
chr2.2 exon 53487234 53487354 53487234 ID=MS.gene071859.t1.exon3;Parent=MS.gene071859.t1
chr2.2 CDS 53487234 53487354 53487234 ID=cds.MS.gene071859.t1;Parent=MS.gene071859.t1
chr2.2 exon 53487455 53487629 53487455 ID=MS.gene071859.t1.exon4;Parent=MS.gene071859.t1
chr2.2 CDS 53487455 53487629 53487455 ID=cds.MS.gene071859.t1;Parent=MS.gene071859.t1
chr2.2 exon 53487720 53487790 53487720 ID=MS.gene071859.t1.exon5;Parent=MS.gene071859.t1
chr2.2 CDS 53487720 53487790 53487720 ID=cds.MS.gene071859.t1;Parent=MS.gene071859.t1
chr2.2 exon 53488239 53488912 53488239 ID=MS.gene071859.t1.exon6;Parent=MS.gene071859.t1
chr2.2 CDS 53488239 53488912 53488239 ID=cds.MS.gene071859.t1;Parent=MS.gene071859.t1
Gene Sequence

>MS.gene071859

ATGCTTGTAAGTACGTTTCACTCTGGTTTCATTTTTTTTTTTTTTGTTAATGCTTTCTTTGCCTTTTGAGTTTTCACCTTAAACTAGTTCCAAATCTGGGACTATATTGCTTTGTTTTTCATTTTAAGGGTCTCTTCAAGTTTTGATTTTTGGTTGGAATTTATATGTTTAAGTCAAAGTTTTTGTGTTTTGTTCATATGGGCCATGTTCAAATTTTATGTATGAGATTTGTTCTTTTGGGTTTTTTTGTTCCTTTTGTTTGGCATGTATATGCCAATGGTGATTGTTGTTAATCAAATTTTAATCCATTTTGATCTGATATATTGATCTGAATAATTTGCATTTTAGTTTAATTTCATCTGAGAATTCACTTTTGAAGTTTTTTCTCATAAGCTCTTGTACCTTATAATATTTTTGTTGAAGTGATTCTCTCATTGATTGGTGTGTGAAAATGAGCATTTTTGTTGCCTAGTTCTTTCATTGTTTTTGCTATCAATTTTCTTGATTCTTCTATTCATTTGTTGTGTTTGTTCATTCTTGTTTCAGATGTGTTCTTAACCTCTGTCAATTTGAAGTGAATTGTTTTCTTCATGAACAAATTAATTATTTGCTTGATTAATTACATTCAATTAATTTGTTTGATCAAATTATTATCCGCGCTGATCACGTGTATGTGATAATGCTTCATAGGTTATGTTTGGCTTTCTATGTTGTTTTAAATCTGGGATACTTTCCTTCTGGTGATTAATTGGTTTATGTGACCTTTTTCAGGTGGCATTGCTCTTGCTTCTTGCATTGAGCAATGCCGTGAATGTTTATGGAGATGTTGAGATATGTCGTTTGAATCCTAAATTGGATCCTAGGCCACACAGTGTCTCTATATTGGAATTTGGTGCAGTTGGAGATGGTAAAACACTGAATACAATTGCATTCCAGAATGCAATTTTCTATCTCAAGTCATTTGCTGATAAGGGTGGTGCTCAGCTCTATGTGCCACCAGGAAACTGGCTTACTGGTAGTTTCAATCTTACTAGCCATCTCACCCTCTTTTTGGAAAAAGGTGCTGTCATTATTGGATCTCAGGTACTTTTACCTCTTGCTTCATTTTTTACCAGTAACTCCTGAAATTTGATTAGTTTCGTCTTTTTTATGTGCGAGGCATTGTCACTATAATCTTTGAATGTATTGAAATTGGGCAAGCATCCCTGAGTAAGAATTTTGTTAGTGGTTTCGTGGACTAAGCTGACTAACAAAAGAGACACGAGGATCAAGCTTATTAAGGGCACACGTATCCAGGGATGTTTATGAAATTTCAATACATTCAAGAATTATAGTGAGACGCTTGTCACCTTCAAGGTCCAAAATGATTTTTTACTCATGGTGAATGTGAAAAATTATAGCGTAGCAGAATGTGAATGAATTGTGTATTGTTCCTATTTGGTACAAAATGTTGTCAAATGGCGGCTATAACAGTGCTATATTATTGTAGCGTATCGGAAATTAAACAAACTACTATTTTCTGTGATTTGCTATTGATAACACTGATTTCTTGTGTATAAAGATATTTCATGTGAAACCAATTTGAGGGTAATTGAGGAGTGGTTTTTCTTGTTTAGGATCCATCTCATTGGAATGTTATTGAAGCTTTACCTTCTTATGGCCGAGGGAAAGATGTTCCTGACGGAAGATACAAGAGCTTGATAAATGGTAACCAGTTGGAAGATGTGGTCATAACAGGTTTGTCAATTCTGCTCCAAATTTGTTCTTGCTTCTATCAAGTTTTTGGCTTTTCTCTTTCTATATTGAATTTTCATATTTTTCATTTAATTTTTACCAGGCAATAATGGAACCATTGATGGCAATGGAATGGTTTGGTGGGACTGGTTTAAGTTTCATTCCCTTAACCACAGTCGTCCTCATCTCGTTGAAATTATATCATCTGATTTTGTGGTAGTTTCAAATCTTACATTCTTGAATGCTCCAGCATATAGCATCCACCCTGTTTATTGCAGGTAAATAATTTTCTGCTTCTTCTTCTTTGATACTTAATTTGGCAGACAAAATTAAGGGATGTTGATAAAGTTACATTTAATTTGATGCAGCAATGTACATATTCAAAACATTTCAATCTCTACTCCTCCAGAATCCCCTTATACTGCCGGTATAGTACCAGGTTAGTAAACATATTAAAGACTCAATTCTGTTATCCTCATTGTGAAATGGCAATGCCTCTATGTAGCATTGACACTTCAGATTGAATGAGTGTTCGGTGTCCAATACATGCTTGTGTCCAACTATGAGGCAAAAACACGGACATCAGACACGATACTGACTCTGACTCGTGGATACCGATAATTATTTGAGATGGTAAAATATTTGAATGTAAGCACACATGTTGGTGTTTTGTCGTGTCAGTGCTCCATAAGTGACTAACATCGACACGACACCGAGTATATTCAATTATATCATTTTTCAAATAATTACTAGGGTTGACGTGTTAGTGTCGTGTCTAATGTCACTGTCTGTACCAGTACTTCATAGACAACCTTTCAAATGTCAATAGCTTACCAAACCTTGGTGAGGGTCACTTAGCTAAACATTTGTGCTCTTCATGTTTTCAGATTCTTCTGATAATGTTTGTATAGAAGATTGTTATGTTTCCATTGGTTTTGACGCAATTTCTCTCAAAAGTGGATGGGATCAGTACGGCATTAAATATGGAAGACCAAGTGAGAAAGTACATATAAGAAGGGTTCATCTTCGTGCATTTACCGGCTCTGCTATTTCATTTGGTACTGAAATGTCTGGTGGAATTTCAAAAGTCCTGATAGAGCATGTTAACATTTTCAACTCAAATAGTGGCATTGAGTTCAGAACAACCAAAGGAAGAGGTGGTTACATAAAAGAAATTGCTTTATCACACATAGAAATGGAAAATGTTTATACAGCAATTTCAGCTAAAGGTAATTCTGGTTCTCATCCTGATGACAAATTTGATCCAAAAGCACTTCCACTTTTGCATCACATAACATTGAAGGATATAACTGGAAAGAATATCACAATTGCTGGAATCTTGAGTGGCATAGAAGAATCACCCTTCACAAACATATGCCTTTCAAATATAACCTTGTCTACAAATTCCGTTTCTTCAAGTAAATGGGAATGTTCAAATGTCTCGGGATTTTCAGACTCGGTGTTGCCTAAACCTTGTCAAGAACTTGGTAACCCTTCAAATTCTTCTTCGTCATCGTGTTACTACCTCATGAGTCTCGGTCAAAATACTGCAGCGATTTAA
Protein sequence

>MS.gene071859.t1

MLVALLLLLALSNAVNVYGDVEICRLNPKLDPRPHSVSILEFGAVGDGKTLNTIAFQNAIFYLKSFADKGGAQLYVPPGNWLTGSFNLTSHLTLFLEKGAVIIGSQDPSHWNVIEALPSYGRGKDVPDGRYKSLINGNQLEDVVITGNNGTIDGNGMVWWDWFKFHSLNHSRPHLVEIISSDFVVVSNLTFLNAPAYSIHPVYCSNVHIQNISISTPPESPYTAGIVPDSSDNVCIEDCYVSIGFDAISLKSGWDQYGIKYGRPSEKVHIRRVHLRAFTGSAISFGTEMSGGISKVLIEHVNIFNSNSGIEFRTTKGRGGYIKEIALSHIEMENVYTAISAKGNSGSHPDDKFDPKALPLLHHITLKDITGKNITIAGILSGIEESPFTNICLSNITLSTNSVSSSKWECSNVSGFSDSVLPKPCQELGNPSNSSSSSCYYLMSLGQNTAAI