Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051768.t1 | XP_024625148.1 | 98.9 | 182 | 2 | 0 | 2 | 183 | 248 | 429 | 3.50E-96 | 360.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051768.t1 | Q9FT45 | 83.0 | 182 | 25 | 1 | 2 | 183 | 242 | 417 | 7.8e-81 | 301.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051768.t1 | A0A072U3I4 | 98.9 | 182 | 2 | 0 | 2 | 183 | 248 | 429 | 2.5e-96 | 360.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051768 | MS.gene071859 | 0.804615 | 1.98E-49 | -1.69E-46 |
| MS.gene051768 | MS.gene23071 | 0.817813 | 2.71E-52 | -1.69E-46 |
| MS.gene051768 | MS.gene25226 | 0.950243 | 2.46E-108 | -1.69E-46 |
| MS.gene051768 | MS.gene28446 | 0.857645 | 1.42E-62 | -1.69E-46 |
| MS.gene051768 | MS.gene38133 | 0.821221 | 4.53E-53 | -1.69E-46 |
| MS.gene051768 | MS.gene46428 | 0.832975 | 6.96E-56 | -1.69E-46 |
| MS.gene051768 | MS.gene58126 | 0.874855 | 4.87E-68 | -1.69E-46 |
| MS.gene051768 | MS.gene63999 | 0.814309 | 1.64E-51 | -1.69E-46 |
| MS.gene051768 | MS.gene64201 | 0.811551 | 6.62E-51 | -1.69E-46 |
| MS.gene051768 | MS.gene64944 | 0.831235 | 1.87E-55 | -1.69E-46 |
| MS.gene051768 | MS.gene70272 | 0.880185 | 6.76E-70 | -1.69E-46 |
| MS.gene051768 | MS.gene75448 | 0.814558 | 1.45E-51 | -1.69E-46 |
| MS.gene051768 | MS.gene79244 | 0.820181 | 7.86E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051768.t1 | MTR_7g106760 | 98.901 | 182 | 2 | 0 | 2 | 183 | 248 | 429 | 7.03e-130 | 372 |
| MS.gene051768.t1 | MTR_4g050170 | 82.418 | 182 | 32 | 0 | 2 | 183 | 262 | 443 | 8.52e-106 | 311 |
| MS.gene051768.t1 | MTR_2g087810 | 76.923 | 182 | 37 | 2 | 2 | 183 | 239 | 415 | 5.81e-97 | 288 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051768.t1 | AT3G52370 | 83.060 | 183 | 23 | 2 | 2 | 183 | 242 | 417 | 2.70e-106 | 311 |
| MS.gene051768.t1 | AT3G52370 | 83.060 | 183 | 23 | 2 | 2 | 183 | 295 | 470 | 3.56e-106 | 313 |
| MS.gene051768.t1 | AT2G35860 | 82.967 | 182 | 26 | 1 | 2 | 183 | 250 | 426 | 1.19e-105 | 310 |
| MS.gene051768.t1 | AT5G06390 | 82.558 | 172 | 30 | 0 | 2 | 173 | 261 | 432 | 3.86e-102 | 301 |
| MS.gene051768.t1 | AT3G11700 | 89.542 | 153 | 16 | 0 | 2 | 154 | 264 | 416 | 5.45e-98 | 291 |
| MS.gene051768.t1 | AT5G05650 | 59.574 | 94 | 34 | 1 | 65 | 158 | 2 | 91 | 5.99e-34 | 117 |
Find 45 sgRNAs with CRISPR-Local
Find 47 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAATGAAATGGCGGATATTC+TGG | 0.243548 | 7.3:+7264623 | MS.gene051768:CDS |
| TATAACGCCGTTAGAAGATT+TGG | 0.248988 | 7.3:+7264831 | MS.gene051768:CDS |
| TCTGACGGTTCTGTCAAATT+TGG | 0.285494 | 7.3:+7264906 | MS.gene051768:CDS |
| AGGATATGTTCTAACAGTTT+TGG | 0.293643 | 7.3:+7264692 | MS.gene051768:CDS |
| TTCGCCATTGCTTCATCATT+TGG | 0.302310 | 7.3:-7264717 | None:intergenic |
| CTACCGTCGGTGTAAATATC+AGG | 0.354622 | 7.3:-7264960 | None:intergenic |
| AACGTCGTTAGCAACGGAAA+TGG | 0.374579 | 7.3:+7264653 | MS.gene051768:CDS |
| ATCCAAACTCTTCTCCATTA+CGG | 0.376670 | 7.3:+7264594 | MS.gene051768:CDS |
| CACCGTAATGGAGAAGAGTT+TGG | 0.378647 | 7.3:-7264596 | None:intergenic |
| GCCTCATAAAGTTGTTGCTC+AGG | 0.394027 | 7.3:+7264881 | MS.gene051768:CDS |
| ATAACGCCGTTAGAAGATTT+GGG | 0.405597 | 7.3:+7264832 | MS.gene051768:CDS |
| TGATAGTATATAATTTGTTC+CGG | 0.408846 | 7.3:-7264774 | None:intergenic |
| TTTCCGTTGCTAACGACGTT+AGG | 0.410525 | 7.3:-7264650 | None:intergenic |
| ACGTCGTTAGCAACGGAAAT+GGG | 0.422550 | 7.3:+7264654 | MS.gene051768:CDS |
| TCCTGAGCAACAACTTTATG+AGG | 0.429700 | 7.3:-7264882 | None:intergenic |
| TCATCTTCATTCTCTTCCTC+CGG | 0.431860 | 7.3:-7265020 | None:intergenic |
| CTCTCCTCCGTTTGATACTC+GGG | 0.454279 | 7.3:-7264804 | None:intergenic |
| ATTAACGACTGATCAATTAT+CGG | 0.461860 | 7.3:+7264740 | MS.gene051768:CDS |
| GACGGTAGAATTTCCGTTCA+AGG | 0.464023 | 7.3:+7264975 | MS.gene051768:CDS |
| ACTCTCCTCCGTTTGATACT+CGG | 0.469204 | 7.3:-7264805 | None:intergenic |
| TTTCATTGTAACCACCGTAA+TGG | 0.480560 | 7.3:-7264608 | None:intergenic |
| AAGAATTCAATACCTCTCCT+TGG | 0.486760 | 7.3:-7265098 | None:intergenic |
| TTACGGTGGTTACAATGAAA+TGG | 0.490556 | 7.3:+7264611 | MS.gene051768:CDS |
| ATTTCCGTTCAAGGTATTGA+CGG | 0.497370 | 7.3:+7264984 | MS.gene051768:CDS |
| CAACGGCGAACGTCAAGTAA+AGG | 0.500147 | 7.3:+7264566 | MS.gene051768:CDS |
| ATAATTTGTTCCGGTGCACC+TGG | 0.520254 | 7.3:-7264765 | None:intergenic |
| ATGGGTCGTTTAGTTTCTGA+AGG | 0.541305 | 7.3:+7264672 | MS.gene051768:CDS |
| TTAGAAGCAACTTTAACAAG+TGG | 0.547256 | 7.3:-7265074 | None:intergenic |
| GGCTCCAAATGATGAAGCAA+TGG | 0.555279 | 7.3:+7264713 | MS.gene051768:CDS |
| TTAAAGTTGCTTCTAAACCA+AGG | 0.557248 | 7.3:+7265081 | MS.gene051768:CDS |
| TATAATTCCCGAGTATCAAA+CGG | 0.563846 | 7.3:+7264797 | MS.gene051768:CDS |
| TCTTCATTCTCTTCCTCCGG+TGG | 0.568260 | 7.3:-7265017 | None:intergenic |
| ATTATCGGAACCAGGTGCAC+CGG | 0.569501 | 7.3:+7264755 | MS.gene051768:CDS |
| TAACCTAACGTCGTTAGCAA+CGG | 0.578368 | 7.3:+7264647 | MS.gene051768:CDS |
| AACACCGTCAATACCTTGAA+CGG | 0.599863 | 7.3:-7264988 | None:intergenic |
| GTTGTTGCTCAGGAATCTGA+CGG | 0.606043 | 7.3:+7264891 | MS.gene051768:CDS |
| GGTTCTGTCAAATTTGGTCA+CGG | 0.607360 | 7.3:+7264912 | MS.gene051768:CDS |
| GTCCACGTCATGCATTTCAA+CGG | 0.632120 | 7.3:+7264549 | None:intergenic |
| ACTGATCAATTATCGGAACC+AGG | 0.656433 | 7.3:+7264747 | MS.gene051768:CDS |
| GTTGCTTCTAAACCAAGGAG+AGG | 0.661362 | 7.3:+7265086 | MS.gene051768:CDS |
| GATCCTGATATTTACACCGA+CGG | 0.666955 | 7.3:+7264957 | MS.gene051768:CDS |
| CAAACTCTTCTCCATTACGG+TGG | 0.680073 | 7.3:+7264597 | MS.gene051768:CDS |
| TTGAACGGAAATTCTACCGT+CGG | 0.682101 | 7.3:-7264973 | None:intergenic |
| CGGTGGTTACAATGAAATGG+CGG | 0.682897 | 7.3:+7264614 | MS.gene051768:CDS |
| AATTCCCGAGTATCAAACGG+AGG | 0.728958 | 7.3:+7264800 | MS.gene051768:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TGATAGTATATAATTTGTTC+CGG | - | chr7.3:7264777-7264796 | None:intergenic | 20.0% |
| ! | ATTAACGACTGATCAATTAT+CGG | + | chr7.3:7264740-7264759 | MS.gene051768:CDS | 25.0% |
| !! | AATTTTCCCAAATCTTCTAA+CGG | - | chr7.3:7264841-7264860 | None:intergenic | 25.0% |
| TATAATTCCCGAGTATCAAA+CGG | + | chr7.3:7264797-7264816 | MS.gene051768:CDS | 30.0% | |
| TTAAAGTTGCTTCTAAACCA+AGG | + | chr7.3:7265081-7265100 | MS.gene051768:CDS | 30.0% | |
| TTAGAAGCAACTTTAACAAG+TGG | - | chr7.3:7265077-7265096 | None:intergenic | 30.0% | |
| ! | AGGATATGTTCTAACAGTTT+TGG | + | chr7.3:7264692-7264711 | MS.gene051768:CDS | 30.0% |
| ATAACGCCGTTAGAAGATTT+GGG | + | chr7.3:7264832-7264851 | MS.gene051768:CDS | 35.0% | |
| ATCCAAACTCTTCTCCATTA+CGG | + | chr7.3:7264594-7264613 | MS.gene051768:CDS | 35.0% | |
| TATAACGCCGTTAGAAGATT+TGG | + | chr7.3:7264831-7264850 | MS.gene051768:CDS | 35.0% | |
| TTACGGTGGTTACAATGAAA+TGG | + | chr7.3:7264611-7264630 | MS.gene051768:CDS | 35.0% | |
| TTTCATTGTAACCACCGTAA+TGG | - | chr7.3:7264611-7264630 | None:intergenic | 35.0% | |
| ! | TAACAAGTGGAGTTTTTGCA+CGG | - | chr7.3:7265064-7265083 | None:intergenic | 35.0% |
| !! | ATTTCCGTTCAAGGTATTGA+CGG | + | chr7.3:7264984-7265003 | MS.gene051768:CDS | 35.0% |
| AACACCGTCAATACCTTGAA+CGG | - | chr7.3:7264991-7265010 | None:intergenic | 40.0% | |
| ACTGATCAATTATCGGAACC+AGG | + | chr7.3:7264747-7264766 | MS.gene051768:CDS | 40.0% | |
| GATCCTGATATTTACACCGA+CGG | + | chr7.3:7264957-7264976 | MS.gene051768:CDS | 40.0% | |
| GGTTCTGTCAAATTTGGTCA+CGG | + | chr7.3:7264912-7264931 | MS.gene051768:CDS | 40.0% | |
| TAACCTAACGTCGTTAGCAA+CGG | + | chr7.3:7264647-7264666 | MS.gene051768:CDS | 40.0% | |
| TCATCTTCATTCTCTTCCTC+CGG | - | chr7.3:7265023-7265042 | None:intergenic | 40.0% | |
| TCCTGAGCAACAACTTTATG+AGG | - | chr7.3:7264885-7264904 | None:intergenic | 40.0% | |
| TCTGACGGTTCTGTCAAATT+TGG | + | chr7.3:7264906-7264925 | MS.gene051768:CDS | 40.0% | |
| TTCGCCATTGCTTCATCATT+TGG | - | chr7.3:7264720-7264739 | None:intergenic | 40.0% | |
| TTGAACGGAAATTCTACCGT+CGG | - | chr7.3:7264976-7264995 | None:intergenic | 40.0% | |
| ! | ATGGGTCGTTTAGTTTCTGA+AGG | + | chr7.3:7264672-7264691 | MS.gene051768:CDS | 40.0% |
| !! | CAATGAAATGGCGGATATTC+TGG | + | chr7.3:7264623-7264642 | MS.gene051768:CDS | 40.0% |
| AACGTCGTTAGCAACGGAAA+TGG | + | chr7.3:7264653-7264672 | MS.gene051768:CDS | 45.0% | |
| AATTCCCGAGTATCAAACGG+AGG | + | chr7.3:7264800-7264819 | MS.gene051768:CDS | 45.0% | |
| ACGTCGTTAGCAACGGAAAT+GGG | + | chr7.3:7264654-7264673 | MS.gene051768:CDS | 45.0% | |
| ACTCTCCTCCGTTTGATACT+CGG | - | chr7.3:7264808-7264827 | None:intergenic | 45.0% | |
| CAAACTCTTCTCCATTACGG+TGG | + | chr7.3:7264597-7264616 | MS.gene051768:CDS | 45.0% | |
| CGGTGGTTACAATGAAATGG+CGG | + | chr7.3:7264614-7264633 | MS.gene051768:CDS | 45.0% | |
| CTACCGTCGGTGTAAATATC+AGG | - | chr7.3:7264963-7264982 | None:intergenic | 45.0% | |
| GACGGTAGAATTTCCGTTCA+AGG | + | chr7.3:7264975-7264994 | MS.gene051768:CDS | 45.0% | |
| GCCTCATAAAGTTGTTGCTC+AGG | + | chr7.3:7264881-7264900 | MS.gene051768:CDS | 45.0% | |
| GGCTCCAAATGATGAAGCAA+TGG | + | chr7.3:7264713-7264732 | MS.gene051768:CDS | 45.0% | |
| GTTGTTGCTCAGGAATCTGA+CGG | + | chr7.3:7264891-7264910 | MS.gene051768:CDS | 45.0% | |
| TTTCCGTTGCTAACGACGTT+AGG | - | chr7.3:7264653-7264672 | None:intergenic | 45.0% | |
| ! | CACCGTAATGGAGAAGAGTT+TGG | - | chr7.3:7264599-7264618 | None:intergenic | 45.0% |
| !! | ATAATTTGTTCCGGTGCACC+TGG | - | chr7.3:7264768-7264787 | None:intergenic | 45.0% |
| ATTATCGGAACCAGGTGCAC+CGG | + | chr7.3:7264755-7264774 | MS.gene051768:CDS | 50.0% | |
| CAACGGCGAACGTCAAGTAA+AGG | + | chr7.3:7264566-7264585 | MS.gene051768:CDS | 50.0% | |
| CTCTCCTCCGTTTGATACTC+GGG | - | chr7.3:7264807-7264826 | None:intergenic | 50.0% | |
| TCTTCATTCTCTTCCTCCGG+TGG | - | chr7.3:7265020-7265039 | None:intergenic | 50.0% | |
| ! | GGAGTTTTTGCACGGCGAAT+AGG | - | chr7.3:7265056-7265075 | None:intergenic | 50.0% |
| ! | TGACGGTGTTCTTTTGCCAC+CGG | + | chr7.3:7265001-7265020 | MS.gene051768:CDS | 50.0% |
| ! | CGGTGTTCTTTTGCCACCGG+AGG | + | chr7.3:7265004-7265023 | MS.gene051768:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 7264558 | 7265107 | 7264558 | ID=MS.gene051768 |
| chr7.3 | mRNA | 7264558 | 7265107 | 7264558 | ID=MS.gene051768.t1;Parent=MS.gene051768 |
| chr7.3 | exon | 7264558 | 7265107 | 7264558 | ID=MS.gene051768.t1.exon1;Parent=MS.gene051768.t1 |
| chr7.3 | CDS | 7264558 | 7265107 | 7264558 | ID=cds.MS.gene051768.t1;Parent=MS.gene051768.t1 |
| Gene Sequence |
| Protein sequence |
