AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene071912

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene071912.t1 MTR_2g008960 93.668 379 19 2 1 379 1 374 0.0 674
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene071912.t1 AT5G64230 42.010 388 176 7 18 375 10 378 9.04e-89 273
MS.gene071912.t1 AT3G19920 32.911 316 163 5 92 371 111 413 3.15e-43 155
MS.gene071912.t1 AT3G19920 32.911 316 163 5 92 371 152 454 3.86e-43 156

Find 122 sgRNAs with CRISPR-Local

Find 193 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGGGCGGTGGCGGATAATTT+AGG 0.162639 2.2:-71418463 MS.gene071912:CDS
TTCAAAGAAGCCAAAGATTT+TGG 0.169588 2.2:-71417128 MS.gene071912:CDS
GAGGAGGGTGTGGAGGGTGG+TGG 0.218739 2.2:-71418614 MS.gene071912:CDS
TACTGCTGATCCTCGTTTGC+AGG 0.243765 2.2:-71418428 MS.gene071912:CDS
CGGGGCATCAATGCCATTAC+TGG 0.280805 2.2:+71417016 None:intergenic
TTCGTCTGCGCCAAAATCTT+TGG 0.282865 2.2:+71417118 None:intergenic
GGGTGTGGAGGGTGGTGGGG+TGG 0.285106 2.2:-71418609 MS.gene071912:CDS
GGAGGGTGTGGAGGGTGGTG+GGG 0.287513 2.2:-71418612 MS.gene071912:CDS
GAGGAGGAGGGTGTGGAGGG+TGG 0.291263 2.2:-71418617 MS.gene071912:CDS
AAGAACAGGTTCTTTCTCTT+TGG 0.297051 2.2:-71416917 MS.gene071912:CDS
GCTTCGCTTGTTAAACTTGC+AGG 0.297214 2.2:-71418406 MS.gene071912:intron
TGGTTTAAGTGGTGGTGTTC+CGG 0.299500 2.2:-71418566 MS.gene071912:CDS
GTGGAGGGTGGTGGGGTGGA+AGG 0.327039 2.2:-71418605 MS.gene071912:CDS
AGGAGGGTGTGGAGGGTGGT+GGG 0.327315 2.2:-71418613 MS.gene071912:CDS
GTTGCTTTGTCTTCAGCATT+TGG 0.333264 2.2:-71418730 MS.gene071912:CDS
TGGTGAGGAGGAGGGTGTGG+AGG 0.336421 2.2:-71418621 MS.gene071912:CDS
GGGCGGTGGCGGATAATTTA+GGG 0.342904 2.2:-71418462 MS.gene071912:CDS
GTGATGGAGGAGAGTGGTAT+AGG 0.347523 2.2:-71418645 MS.gene071912:CDS
AATTGGCAGCGGAGTTGGTT+TGG 0.367956 2.2:-71418537 MS.gene071912:CDS
TTGCTTTGTCTTCAGCATTT+GGG 0.372512 2.2:-71418729 MS.gene071912:CDS
GTGGAAGGTGAAGGGAGGTG+AGG 0.381406 2.2:-71418590 MS.gene071912:CDS
GGATGATGAAACGGAGGGTT+TGG 0.381540 2.2:+71419019 None:intergenic
CTCTCATTGATGCTCAATGC+CGG 0.381820 2.2:+71416996 None:intergenic
GGATGGTGAGGAGGAGGGTG+TGG 0.384248 2.2:-71418624 MS.gene071912:CDS
GCATAACATGATTGGAGGTT+TGG 0.384676 2.2:+71416858 None:intergenic
GGTGAAAAGGGAAGAGATTA+GGG 0.395429 2.2:+71419101 None:intergenic
GAATGAAACGGAGGAGGAAG+AGG 0.397994 2.2:+71418986 None:intergenic
TGGTGTTCCGGCGGAGAAAT+TGG 0.402368 2.2:-71418554 MS.gene071912:CDS
GGTGAGGAGGAGGGTGTGGA+GGG 0.415395 2.2:-71418620 MS.gene071912:CDS
TGGTGAAAAGGGAAGAGATT+AGG 0.417039 2.2:+71419100 None:intergenic
GGTATAGGATGGTGAGGAGG+AGG 0.417760 2.2:-71418630 MS.gene071912:CDS
TCTCATTGATGCTCAATGCC+GGG 0.418765 2.2:+71416997 None:intergenic
GATCAAGGAGAGGGTGAAGA+AGG 0.422863 2.2:-71418667 MS.gene071912:CDS
CATGGCTGCCATTGTTGTGC+AGG 0.422978 2.2:-71417040 MS.gene071912:CDS
GGTGGTGGGGTGGAAGGTGA+AGG 0.423207 2.2:-71418599 MS.gene071912:CDS
GGCTGAGTTGGAGAGGATGT+TGG 0.432888 2.2:-71416973 MS.gene071912:CDS
GTGGGGTGGAAGGTGAAGGG+AGG 0.443740 2.2:-71418595 MS.gene071912:CDS
CAACGGCGGTGGTGGTGAAA+AGG 0.444067 2.2:+71419088 None:intergenic
TGGAAGGTGAAGGGAGGTGA+GGG 0.448134 2.2:-71418589 MS.gene071912:CDS
CATCAATGAGAGGGCTGAGT+TGG 0.451437 2.2:-71416985 MS.gene071912:CDS
GGAGAAATTGGCAGCGGAGT+TGG 0.458127 2.2:-71418542 MS.gene071912:CDS
TGTAGTTGACAGAGAAGGAG+AGG 0.458499 2.2:+71419049 None:intergenic
AGGATGAGCAATGTGTAAAA+TGG 0.472912 2.2:+71416889 None:intergenic
GTTCGGAGGAGCGGCGGAAG+TGG 0.474833 2.2:+71419123 None:intergenic
GGAAAGCATTATAGAGGGGT+TGG 0.475155 2.2:-71416952 MS.gene071912:CDS
GTTGACAGAGAAGGAGAGGA+AGG 0.478361 2.2:+71419053 None:intergenic
AGGAGCGGCGGAAGTGGTAG+TGG 0.486154 2.2:+71419129 None:intergenic
TCCAATCATGTTATGCTCGC+TGG 0.490686 2.2:-71416851 MS.gene071912:CDS
GTATAGGATGGTGAGGAGGA+GGG 0.494671 2.2:-71418629 MS.gene071912:CDS
CGAAGCGGTTCGATCGTTAA+CGG 0.496200 2.2:-71418812 MS.gene071912:CDS
CTGATGAAGCACTTCGGAGA+TGG 0.497363 2.2:-71418483 MS.gene071912:CDS
AAGGTGGCGGAGAGAGATCA+AGG 0.501719 2.2:-71418682 MS.gene071912:CDS
GGAGGTGAGGGTGGTTTAAG+TGG 0.502354 2.2:-71418577 MS.gene071912:CDS
GGAGGAAGAGGTAGAAAGTG+AGG 0.503057 2.2:+71418998 None:intergenic
AGAAAGTGAGGATGATGAAA+CGG 0.504841 2.2:+71419010 None:intergenic
GAGGGTGAAGAAGGTGATGG+AGG 0.506425 2.2:-71418658 MS.gene071912:CDS
ACTTCGGAGATGGGCGGTGG+CGG 0.507790 2.2:-71418473 MS.gene071912:CDS
AAGAAGGAATGAAACGGAGG+AGG 0.508172 2.2:+71418980 None:intergenic
CTTTGTCTTCAGCATTTGGG+AGG 0.510290 2.2:-71418726 MS.gene071912:CDS
AGAGAAGGAGAGGAAGGTGG+TGG 0.511463 2.2:+71419059 None:intergenic
GATGTTGGAAAGCATTATAG+AGG 0.512128 2.2:-71416958 MS.gene071912:CDS
CAGTAATGGCATTGATGCCC+CGG 0.513836 2.2:-71417015 MS.gene071912:CDS
AACGGCGGTGGTGGTGAAAA+GGG 0.514502 2.2:+71419089 None:intergenic
GGAGAGGGTGAAGAAGGTGA+TGG 0.514958 2.2:-71418661 MS.gene071912:CDS
GTGGTGGGGTGGAAGGTGAA+GGG 0.517185 2.2:-71418598 MS.gene071912:CDS
ACCAGCGAGCATAACATGAT+TGG 0.520683 2.2:+71416850 None:intergenic
GAGAGTGGTATAGGATGGTG+AGG 0.520943 2.2:-71418636 MS.gene071912:CDS
GAGTTTGTAGTTGACAGAGA+AGG 0.525446 2.2:+71419044 None:intergenic
AGCGAGCATAACATGATTGG+AGG 0.527880 2.2:+71416853 None:intergenic
GGAACAAGAGAAGCAAGAAC+AGG 0.529481 2.2:-71416931 MS.gene071912:CDS
GAGATTAGGGTTCGGAGGAG+CGG 0.531244 2.2:+71419114 None:intergenic
GGCAGCGGAGTTGGTTTGGA+TGG 0.531399 2.2:-71418533 MS.gene071912:CDS
AGTGGTATAGGATGGTGAGG+AGG 0.536537 2.2:-71418633 MS.gene071912:CDS
GACAGAGAAGGAGAGGAAGG+TGG 0.536632 2.2:+71419056 None:intergenic
TGATGAAGCACTTCGGAGAT+GGG 0.536824 2.2:-71418482 MS.gene071912:CDS
TCCGCTGCCAATTTCTCCGC+CGG 0.537453 2.2:+71418547 None:intergenic
AGCACTTCGGAGATGGGCGG+TGG 0.537503 2.2:-71418476 MS.gene071912:CDS
CACATTGCTCATCCTCTGAC+TGG 0.539240 2.2:-71416881 MS.gene071912:CDS
AAGGTGAAGGGAGGTGAGGG+TGG 0.541781 2.2:-71418586 MS.gene071912:CDS
AAGAAGGTGATGGAGGAGAG+TGG 0.541790 2.2:-71418651 MS.gene071912:CDS
TGGGAGGGCGTTGAGGCAGT+TGG 0.543117 2.2:-71418710 MS.gene071912:CDS
AAAGGGAAGAGATTAGGGTT+CGG 0.546803 2.2:+71419106 None:intergenic
ATGTTGGAAAGCATTATAGA+GGG 0.549563 2.2:-71416957 MS.gene071912:CDS
CAGCATTTGGGAGGGCGTTG+AGG 0.554300 2.2:-71418717 MS.gene071912:CDS
CAAGATGAAGCAATGCATGC+AGG 0.557965 2.2:-71417084 MS.gene071912:CDS
CGGCGGAAGTGGTAGTGGCA+TGG 0.558032 2.2:+71419134 None:intergenic
ATTAGGGTTCGGAGGAGCGG+CGG 0.562847 2.2:+71419117 None:intergenic
CTCATTGATGCTCAATGCCG+GGG 0.565754 2.2:+71416998 None:intergenic
TTTGTCTTCAGCATTTGGGA+GGG 0.565879 2.2:-71418725 MS.gene071912:CDS
TTGTTGTGCAGGGCCAGTAA+TGG 0.566356 2.2:-71417029 MS.gene071912:CDS
GTAACGAATGAACACCTTCG+AGG 0.580463 2.2:+71418767 None:intergenic
GGGAAGAGATTAGGGTTCGG+AGG 0.585432 2.2:+71419109 None:intergenic
ATGAGAGGGCTGAGTTGGAG+AGG 0.589910 2.2:-71416980 MS.gene071912:CDS
ATCGATGATAATCACCTCGA+AGG 0.591758 2.2:-71418781 MS.gene071912:CDS
GGTGAGGGTGGTTTAAGTGG+TGG 0.593095 2.2:-71418574 MS.gene071912:CDS
AAGTGAGGATGATGAAACGG+AGG 0.593185 2.2:+71419013 None:intergenic
GTTGAGGCAGTTGGAAGTGA+AGG 0.595100 2.2:-71418701 MS.gene071912:CDS
CGGCATTGAGCATCAATGAG+AGG 0.597450 2.2:-71416995 MS.gene071912:CDS
GCGGAGAGAGATCAAGGAGA+GGG 0.598505 2.2:-71418676 MS.gene071912:CDS
TGTTGGAAAGCATTATAGAG+GGG 0.598822 2.2:-71416956 MS.gene071912:CDS
ACAAGCGAAGCCTGCAAACG+AGG 0.601298 2.2:+71418418 None:intergenic
GGCATTGAGCATCAATGAGA+GGG 0.602387 2.2:-71416994 MS.gene071912:CDS
TTGGAGGTTTGGCCAGTCAG+AGG 0.607532 2.2:+71416869 None:intergenic
GGTTTGGATGGTGAAGAAGA+TGG 0.612162 2.2:-71418521 MS.gene071912:CDS
CTGAGAAAGAGCACGTCCGA+TGG 0.614254 2.2:+71418879 None:intergenic
GAGGCAGTTGGAAGTGAAGG+TGG 0.615671 2.2:-71418698 MS.gene071912:CDS
TCCGGCGGAGAAATTGGCAG+CGG 0.615724 2.2:-71418548 MS.gene071912:CDS
GTGAAGAAGATGGTGGAGTG+TGG 0.615961 2.2:-71418511 MS.gene071912:CDS
ATGGCTGCCATTGTTGTGCA+GGG 0.617221 2.2:-71417039 MS.gene071912:CDS
AGTGAGGATGATGAAACGGA+GGG 0.622730 2.2:+71419014 None:intergenic
GGCGGAGAGAGATCAAGGAG+AGG 0.623247 2.2:-71418677 MS.gene071912:CDS
GCAGTTGGAAGTGAAGGTGG+CGG 0.625405 2.2:-71418695 MS.gene071912:CDS
ACACGAAACGACACTGCCAT+CGG 0.626709 2.2:-71418895 MS.gene071912:CDS
TTACTGGCCCTGCACAACAA+TGG 0.626932 2.2:+71417032 None:intergenic
TTTAAGTGGTGGTGTTCCGG+CGG 0.628276 2.2:-71418563 MS.gene071912:CDS
ATGAGCAATGTGTAAAATGG+TGG 0.634351 2.2:+71416892 None:intergenic
TTGGATGGTGAAGAAGATGG+TGG 0.645396 2.2:-71418518 MS.gene071912:CDS
TGGAGGAGAGTGGTATAGGA+TGG 0.647947 2.2:-71418641 MS.gene071912:CDS
AGTTAAAGATGTTACAGACA+TGG 0.655695 2.2:-71417058 MS.gene071912:CDS
TGAAGCACTTCGGAGATGGG+CGG 0.665635 2.2:-71418479 MS.gene071912:CDS
TAGTTCAAGAAAATACGAAG+CGG 0.688958 2.2:-71418827 MS.gene071912:CDS
GTTGTGCTGATGAAGCACTT+CGG 0.693143 2.2:-71418489 MS.gene071912:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ACTTTTTAAATCATTTAAAA+TGG - chr2.2:71418223-71418242 MS.gene071912:intron 10.0%
!!! GATTTTTTAAATAGATATAG+TGG - chr2.2:71418276-71418295 MS.gene071912:intron 15.0%
!!! TTTAAATCATTTAAAATGGT+TGG - chr2.2:71418227-71418246 MS.gene071912:intron 15.0%
!! ACATAAGAAAATATGAAGAA+TGG + chr2.2:71417609-71417628 None:intergenic 20.0%
!! ATATGAAGAATGGATATATA+TGG + chr2.2:71417599-71417618 None:intergenic 20.0%
!! ATTTATGATCAAGAATCTAA+TGG + chr2.2:71418512-71418531 None:intergenic 20.0%
!! TACAGAGTAGTTAAATTTAT+AGG - chr2.2:71418759-71418778 MS.gene071912:CDS 20.0%
!! TCAAAGCATAAACTAAAAAT+TGG + chr2.2:71418590-71418609 None:intergenic 20.0%
!! TCTTATTGTATGTATAACTT+TGG + chr2.2:71418181-71418200 None:intergenic 20.0%
!!! AAATGTATTTCTAGTTAAAG+TGG - chr2.2:71418609-71418628 MS.gene071912:CDS 20.0%
!!! AATGTATTTCTAGTTAAAGT+GGG - chr2.2:71418610-71418629 MS.gene071912:CDS 20.0%
!!! TAATTTTACAATTTTGACCA+TGG - chr2.2:71417655-71417674 MS.gene071912:intron 20.0%
!!! TTAGTTTAAAGTTAAAACCA+TGG + chr2.2:71417675-71417694 None:intergenic 20.0%
!!! TTTTCATTGAAGAAAAAAGA+AGG + chr2.2:71417016-71417035 None:intergenic 20.0%
! AATGGCCAATAAACTTATTA+TGG - chr2.2:71418387-71418406 MS.gene071912:intron 25.0%
! AGAAAAAAGAAGGAATGAAA+CGG + chr2.2:71417006-71417025 None:intergenic 25.0%
! ATGGCCAATAAACTTATTAT+GGG - chr2.2:71418388-71418407 MS.gene071912:intron 25.0%
! CCATCTTTATCAAAATTACT+TGG - chr2.2:71417739-71417758 MS.gene071912:intron 25.0%
! TAAACTAAAAATTGGATGAG+AGG + chr2.2:71418582-71418601 None:intergenic 25.0%
! TAACTTTGGATATAATTTCC+CGG + chr2.2:71418167-71418186 None:intergenic 25.0%
! TTGATCATAAATAACTTCCA+AGG - chr2.2:71418520-71418539 MS.gene071912:CDS 25.0%
! TTTATTGGCCATTATAAACT+TGG + chr2.2:71418380-71418399 None:intergenic 25.0%
!!! CCAAGTAATTTTGATAAAGA+TGG + chr2.2:71417742-71417761 None:intergenic 25.0%
!!! GTTCAAATTTAGAAGTGTTT+AGG - chr2.2:71418663-71418682 MS.gene071912:CDS 25.0%
AAAATATTAGCTCGAGAATG+TGG - chr2.2:71417782-71417801 MS.gene071912:intron 30.0%
AACTTTGGATATAATTTCCC+GGG + chr2.2:71418166-71418185 None:intergenic 30.0%
AAGCCCCATAATAAGTTTAT+TGG + chr2.2:71418395-71418414 None:intergenic 30.0%
AGTTAAAGATGTTACAGACA+TGG - chr2.2:71418919-71418938 MS.gene071912:CDS 30.0%
GCGAACATTAAAGATATCAT+AGG + chr2.2:71418793-71418812 None:intergenic 30.0%
TAAATAACTTCCAAGGATTG+TGG - chr2.2:71418527-71418546 MS.gene071912:CDS 30.0%
TAGTTCAAGAAAATACGAAG+CGG - chr2.2:71417150-71417169 MS.gene071912:intron 30.0%
TCTTGTCTCCAAGTTTATAA+TGG - chr2.2:71418369-71418388 MS.gene071912:intron 30.0%
TGGCCAATAAACTTATTATG+GGG - chr2.2:71418389-71418408 MS.gene071912:intron 30.0%
TTTAAGCCTTTGTACTCATT+AGG - chr2.2:71418120-71418139 MS.gene071912:intron 30.0%
TTTCAAACCACCTATATTCT+CGG + chr2.2:71417765-71417784 None:intergenic 30.0%
TTTGAACCACTATGAAGTAT+TGG + chr2.2:71418650-71418669 None:intergenic 30.0%
! TCGTATTTTCTTGAACTAAC+TGG + chr2.2:71417148-71417167 None:intergenic 30.0%
! TTCAAAGAAGCCAAAGATTT+TGG - chr2.2:71418849-71418868 MS.gene071912:CDS 30.0%
!! ATGTTGGAAAGCATTATAGA+GGG - chr2.2:71419020-71419039 MS.gene071912:CDS 30.0%
!! TTTTGTCTTGTTGCATTACA+AGG + chr2.2:71417908-71417927 None:intergenic 30.0%
AAAAAGAAGGAATGAAACGG+AGG + chr2.2:71417003-71417022 None:intergenic 35.0%
AAGAACAGGTTCTTTCTCTT+TGG - chr2.2:71419060-71419079 MS.gene071912:CDS 35.0%
AATTACTTGGCCGAGAATAT+AGG - chr2.2:71417752-71417771 MS.gene071912:intron 35.0%
AGAAAGTGAGGATGATGAAA+CGG + chr2.2:71416970-71416989 None:intergenic 35.0%
AGGATGAGCAATGTGTAAAA+TGG + chr2.2:71419091-71419110 None:intergenic 35.0%
ATGACTCCAATACTTCATAG+TGG - chr2.2:71418641-71418660 MS.gene071912:CDS 35.0%
ATGAGCAATGTGTAAAATGG+TGG + chr2.2:71419088-71419107 None:intergenic 35.0%
CCAACTTGTAATTCTAGCAA+GGG - chr2.2:71417938-71417957 MS.gene071912:intron 35.0%
CTAATGCCTAATGAGTACAA+AGG + chr2.2:71418129-71418148 None:intergenic 35.0%
TCCAACTTGTAATTCTAGCA+AGG - chr2.2:71417937-71417956 MS.gene071912:intron 35.0%
TTAAGACAAATCGTTGCCTT+TGG + chr2.2:71418087-71418106 None:intergenic 35.0%
TTGCTTTGTCTTCAGCATTT+GGG - chr2.2:71417248-71417267 MS.gene071912:intron 35.0%
! ATAGTAGCTTTTGACACCAA+AGG - chr2.2:71418068-71418087 MS.gene071912:intron 35.0%
! GAACTTATTCTGCGTTTTGA+TGG + chr2.2:71417053-71417072 None:intergenic 35.0%
! TTAGATTAGACTAGTTGTCC+CGG - chr2.2:71418145-71418164 MS.gene071912:intron 35.0%
!! GATGTTGGAAAGCATTATAG+AGG - chr2.2:71419019-71419038 MS.gene071912:CDS 35.0%
!! TGTTGGAAAGCATTATAGAG+GGG - chr2.2:71419021-71419040 MS.gene071912:CDS 35.0%
!! TTGGAAAATTTCTGAGGCAA+AGG - chr2.2:71417816-71417835 MS.gene071912:intron 35.0%
AAAGGGAAGAGATTAGGGTT+CGG + chr2.2:71416874-71416893 None:intergenic 40.0%
ATCGATGATAATCACCTCGA+AGG - chr2.2:71417196-71417215 MS.gene071912:intron 40.0%
CACTTTGTCAAAGCTGTTTG+TGG - chr2.2:71417871-71417890 MS.gene071912:intron 40.0%
CCCTTGCTAGAATTACAAGT+TGG + chr2.2:71417941-71417960 None:intergenic 40.0%
CTTTGAACAGAAATGCTGCT+TGG + chr2.2:71418836-71418855 None:intergenic 40.0%
GAGAGGTAACTCTTCATTCT+AGG + chr2.2:71418565-71418584 None:intergenic 40.0%
GAGTTTGTAGTTGACAGAGA+AGG + chr2.2:71416936-71416955 None:intergenic 40.0%
GCAAAGGAGGAAAGATGATA+AGG - chr2.2:71417832-71417851 MS.gene071912:intron 40.0%
GCATAACATGATTGGAGGTT+TGG + chr2.2:71419122-71419141 None:intergenic 40.0%
GGTGAAAAGGGAAGAGATTA+GGG + chr2.2:71416879-71416898 None:intergenic 40.0%
TGGTGAAAAGGGAAGAGATT+AGG + chr2.2:71416880-71416899 None:intergenic 40.0%
TTTCAGTCACAGCAACATTC+TGG - chr2.2:71418341-71418360 MS.gene071912:intron 40.0%
TTTGTCTTCAGCATTTGGGA+GGG - chr2.2:71417252-71417271 MS.gene071912:intron 40.0%
! GGTAGGTTGGAAAATTTCTG+AGG - chr2.2:71417810-71417829 MS.gene071912:intron 40.0%
! GTTGCTTTGTCTTCAGCATT+TGG - chr2.2:71417247-71417266 MS.gene071912:intron 40.0%
! TAGATTAGACTAGTTGTCCC+GGG - chr2.2:71418146-71418165 MS.gene071912:intron 40.0%
!! CTTATTCTGCGTTTTGATGG+TGG + chr2.2:71417050-71417069 None:intergenic 40.0%
!! GAAAATTTCTGAGGCAAAGG+AGG - chr2.2:71417819-71417838 MS.gene071912:intron 40.0%
AAGAACGCGAAAAACACGGA+CGG - chr2.2:71416812-71416831 MS.gene071912:CDS 45.0%
AAGAAGGAATGAAACGGAGG+AGG + chr2.2:71417000-71417019 None:intergenic 45.0%
AAGTGAGGATGATGAAACGG+AGG + chr2.2:71416967-71416986 None:intergenic 45.0%
ACCAGCGAGCATAACATGAT+TGG + chr2.2:71419130-71419149 None:intergenic 45.0%
AGCGAGCATAACATGATTGG+AGG + chr2.2:71419127-71419146 None:intergenic 45.0%
AGTGAGGATGATGAAACGGA+GGG + chr2.2:71416966-71416985 None:intergenic 45.0%
CAAGATGAAGCAATGCATGC+AGG - chr2.2:71418893-71418912 MS.gene071912:CDS 45.0%
CTCTCATTGATGCTCAATGC+CGG + chr2.2:71418984-71419003 None:intergenic 45.0%
CTTTGTCTTCAGCATTTGGG+AGG - chr2.2:71417251-71417270 MS.gene071912:intron 45.0%
GGAACAAGAGAAGCAAGAAC+AGG - chr2.2:71419046-71419065 MS.gene071912:CDS 45.0%
GGTTTGGATGGTGAAGAAGA+TGG - chr2.2:71417456-71417475 MS.gene071912:intron 45.0%
GTAACGAATGAACACCTTCG+AGG + chr2.2:71417213-71417232 None:intergenic 45.0%
TACTTGGCCGAGAATATAGG+TGG - chr2.2:71417755-71417774 MS.gene071912:intron 45.0%
TCCAATCATGTTATGCTCGC+TGG - chr2.2:71419126-71419145 MS.gene071912:CDS 45.0%
TCTCATTGATGCTCAATGCC+GGG + chr2.2:71418983-71419002 None:intergenic 45.0%
TGTAGTTGACAGAGAAGGAG+AGG + chr2.2:71416931-71416950 None:intergenic 45.0%
TTCGTCTGCGCCAAAATCTT+TGG + chr2.2:71418862-71418881 None:intergenic 45.0%
TTGGATGGTGAAGAAGATGG+TGG - chr2.2:71417459-71417478 MS.gene071912:intron 45.0%
! GCTTCGCTTGTTAAACTTGC+AGG - chr2.2:71417571-71417590 MS.gene071912:intron 45.0%
! GGCATTGAGCATCAATGAGA+GGG - chr2.2:71418983-71419002 MS.gene071912:CDS 45.0%
! GTTGTGCTGATGAAGCACTT+CGG - chr2.2:71417488-71417507 MS.gene071912:intron 45.0%
! TGATGAAGCACTTCGGAGAT+GGG - chr2.2:71417495-71417514 MS.gene071912:intron 45.0%
! TGGTTTAAGTGGTGGTGTTC+CGG - chr2.2:71417411-71417430 MS.gene071912:intron 45.0%
! TTTTGGCGCAGACGAAATTG+AGG - chr2.2:71418866-71418885 MS.gene071912:CDS 45.0%
!! GGAAAGCATTATAGAGGGGT+TGG - chr2.2:71419025-71419044 MS.gene071912:CDS 45.0%
!!! GAAGGTGGTGGTTTTGTCAA+CGG + chr2.2:71416909-71416928 None:intergenic 45.0%
ACACGAAACGACACTGCCAT+CGG - chr2.2:71417082-71417101 MS.gene071912:CDS 50.0%
AGTGGTATAGGATGGTGAGG+AGG - chr2.2:71417344-71417363 MS.gene071912:intron 50.0%
ATGGCTGCCATTGTTGTGCA+GGG - chr2.2:71418938-71418957 MS.gene071912:CDS 50.0%
CACATTGCTCATCCTCTGAC+TGG - chr2.2:71419096-71419115 MS.gene071912:CDS 50.0%
CATCAATGAGAGGGCTGAGT+TGG - chr2.2:71418992-71419011 MS.gene071912:CDS 50.0%
CGAAGCGGTTCGATCGTTAA+CGG - chr2.2:71417165-71417184 MS.gene071912:intron 50.0%
CTCATTGATGCTCAATGCCG+GGG + chr2.2:71418982-71419001 None:intergenic 50.0%
GAATGAAACGGAGGAGGAAG+AGG + chr2.2:71416994-71417013 None:intergenic 50.0%
GAATGTGGACGTAGGTAGGT+TGG - chr2.2:71417797-71417816 MS.gene071912:intron 50.0%
GAGAGTGGTATAGGATGGTG+AGG - chr2.2:71417341-71417360 MS.gene071912:intron 50.0%
GATCAAGGAGAGGGTGAAGA+AGG - chr2.2:71417310-71417329 MS.gene071912:intron 50.0%
GGAGGAAGAGGTAGAAAGTG+AGG + chr2.2:71416982-71417001 None:intergenic 50.0%
GGATGATGAAACGGAGGGTT+TGG + chr2.2:71416961-71416980 None:intergenic 50.0%
GGGGAAGAACGCGAAAAACA+CGG - chr2.2:71416808-71416827 MS.gene071912:CDS 50.0%
GTATAGGATGGTGAGGAGGA+GGG - chr2.2:71417348-71417367 MS.gene071912:intron 50.0%
GTTGACAGAGAAGGAGAGGA+AGG + chr2.2:71416927-71416946 None:intergenic 50.0%
GTTGAGGCAGTTGGAAGTGA+AGG - chr2.2:71417276-71417295 MS.gene071912:intron 50.0%
TACTGCTGATCCTCGTTTGC+AGG - chr2.2:71417549-71417568 MS.gene071912:intron 50.0%
TAGCTCGAGAATGTGGACGT+AGG - chr2.2:71417789-71417808 MS.gene071912:intron 50.0%
TCGAGAATGTGGACGTAGGT+AGG - chr2.2:71417793-71417812 MS.gene071912:intron 50.0%
TGCAGAGAGACCACAATCCT+TGG + chr2.2:71418540-71418559 None:intergenic 50.0%
TGGAGGAGAGTGGTATAGGA+TGG - chr2.2:71417336-71417355 MS.gene071912:intron 50.0%
TTACTGGCCCTGCACAACAA+TGG + chr2.2:71418948-71418967 None:intergenic 50.0%
TTGTTGTGCAGGGCCAGTAA+TGG - chr2.2:71418948-71418967 MS.gene071912:CDS 50.0%
! AATTGGCAGCGGAGTTGGTT+TGG - chr2.2:71417440-71417459 MS.gene071912:intron 50.0%
! CGGCATTGAGCATCAATGAG+AGG - chr2.2:71418982-71419001 MS.gene071912:CDS 50.0%
! CTGATGAAGCACTTCGGAGA+TGG - chr2.2:71417494-71417513 MS.gene071912:intron 50.0%
! GTGATGGAGGAGAGTGGTAT+AGG - chr2.2:71417332-71417351 MS.gene071912:intron 50.0%
! TTTAAGTGGTGGTGTTCCGG+CGG - chr2.2:71417414-71417433 MS.gene071912:intron 50.0%
!! AAGAAGGTGATGGAGGAGAG+TGG - chr2.2:71417326-71417345 MS.gene071912:intron 50.0%
!! CAGTAATGGCATTGATGCCC+CGG - chr2.2:71418962-71418981 MS.gene071912:CDS 50.0%
!! GTGAAGAAGATGGTGGAGTG+TGG - chr2.2:71417466-71417485 MS.gene071912:intron 50.0%
AACGCGAAAAACACGGACGG+CGG - chr2.2:71416815-71416834 MS.gene071912:CDS 55.0%
ACAAGCGAAGCCTGCAAACG+AGG + chr2.2:71417562-71417581 None:intergenic 55.0%
AGAGAAGGAGAGGAAGGTGG+TGG + chr2.2:71416921-71416940 None:intergenic 55.0%
CATGGCTGCCATTGTTGTGC+AGG - chr2.2:71418937-71418956 MS.gene071912:CDS 55.0%
GACAGAGAAGGAGAGGAAGG+TGG + chr2.2:71416924-71416943 None:intergenic 55.0%
GAGATTAGGGTTCGGAGGAG+CGG + chr2.2:71416866-71416885 None:intergenic 55.0%
GAGGCAGTTGGAAGTGAAGG+TGG - chr2.2:71417279-71417298 MS.gene071912:intron 55.0%
GCAGTTGGAAGTGAAGGTGG+CGG - chr2.2:71417282-71417301 MS.gene071912:intron 55.0%
GCGGAGAGAGATCAAGGAGA+GGG - chr2.2:71417301-71417320 MS.gene071912:intron 55.0%
GGAGAAATTGGCAGCGGAGT+TGG - chr2.2:71417435-71417454 MS.gene071912:intron 55.0%
GGAGAGGGTGAAGAAGGTGA+TGG - chr2.2:71417316-71417335 MS.gene071912:intron 55.0%
GGAGGTGAGGGTGGTTTAAG+TGG - chr2.2:71417400-71417419 MS.gene071912:intron 55.0%
GGGAAGAGATTAGGGTTCGG+AGG + chr2.2:71416871-71416890 None:intergenic 55.0%
GGTATAGGATGGTGAGGAGG+AGG - chr2.2:71417347-71417366 MS.gene071912:intron 55.0%
GGTGAGGGTGGTTTAAGTGG+TGG - chr2.2:71417403-71417422 MS.gene071912:intron 55.0%
TGGAAGGTGAAGGGAGGTGA+GGG - chr2.2:71417388-71417407 MS.gene071912:intron 55.0%
TGGTGTTCCGGCGGAGAAAT+TGG - chr2.2:71417423-71417442 MS.gene071912:intron 55.0%
TTGGAGGTTTGGCCAGTCAG+AGG + chr2.2:71419111-71419130 None:intergenic 55.0%
! AACGGCGGTGGTGGTGAAAA+GGG + chr2.2:71416891-71416910 None:intergenic 55.0%
! ATGAGAGGGCTGAGTTGGAG+AGG - chr2.2:71418997-71419016 MS.gene071912:CDS 55.0%
! CGGGGCATCAATGCCATTAC+TGG + chr2.2:71418964-71418983 None:intergenic 55.0%
! CTGAGAAAGAGCACGTCCGA+TGG + chr2.2:71417101-71417120 None:intergenic 55.0%
! GAGGGTGAAGAAGGTGATGG+AGG - chr2.2:71417319-71417338 MS.gene071912:intron 55.0%
! GGCTGAGTTGGAGAGGATGT+TGG - chr2.2:71419004-71419023 MS.gene071912:CDS 55.0%
! TGAAGCACTTCGGAGATGGG+CGG - chr2.2:71417498-71417517 MS.gene071912:intron 55.0%
!! AAGGTGGCGGAGAGAGATCA+AGG - chr2.2:71417295-71417314 MS.gene071912:intron 55.0%
!! GGGCGGTGGCGGATAATTTA+GGG - chr2.2:71417515-71417534 MS.gene071912:intron 55.0%
!! TGGGCGGTGGCGGATAATTT+AGG - chr2.2:71417514-71417533 MS.gene071912:intron 55.0%
!!! GGTGGTGGTTTTGTCAACGG+CGG + chr2.2:71416906-71416925 None:intergenic 55.0%
AAGGTGAAGGGAGGTGAGGG+TGG - chr2.2:71417391-71417410 MS.gene071912:intron 60.0%
ATTAGGGTTCGGAGGAGCGG+CGG + chr2.2:71416863-71416882 None:intergenic 60.0%
GGCGGAGAGAGATCAAGGAG+AGG - chr2.2:71417300-71417319 MS.gene071912:intron 60.0%
GTGGAAGGTGAAGGGAGGTG+AGG - chr2.2:71417387-71417406 MS.gene071912:intron 60.0%
TCCGCTGCCAATTTCTCCGC+CGG + chr2.2:71417433-71417452 None:intergenic 60.0%
TCCGGCGGAGAAATTGGCAG+CGG - chr2.2:71417429-71417448 MS.gene071912:intron 60.0%
! CAACGGCGGTGGTGGTGAAA+AGG + chr2.2:71416892-71416911 None:intergenic 60.0%
! GTGGTGGGGTGGAAGGTGAA+GGG - chr2.2:71417379-71417398 MS.gene071912:intron 60.0%
!! CAGCATTTGGGAGGGCGTTG+AGG - chr2.2:71417260-71417279 MS.gene071912:intron 60.0%
!! GGCAGCGGAGTTGGTTTGGA+TGG - chr2.2:71417444-71417463 MS.gene071912:intron 60.0%
!! GGTTTTGTCAACGGCGGTGG+TGG + chr2.2:71416900-71416919 None:intergenic 60.0%
!!! GGTGGTTTTGTCAACGGCGG+TGG + chr2.2:71416903-71416922 None:intergenic 60.0%
ACTTCGGAGATGGGCGGTGG+CGG - chr2.2:71417504-71417523 MS.gene071912:intron 65.0%
AGCACTTCGGAGATGGGCGG+TGG - chr2.2:71417501-71417520 MS.gene071912:intron 65.0%
AGGAGCGGCGGAAGTGGTAG+TGG + chr2.2:71416851-71416870 None:intergenic 65.0%
GCGAAAAACACGGACGGCGG+CGG - chr2.2:71416818-71416837 MS.gene071912:CDS 65.0%
GGATGGTGAGGAGGAGGGTG+TGG - chr2.2:71417353-71417372 MS.gene071912:intron 65.0%
GTGGGGTGGAAGGTGAAGGG+AGG - chr2.2:71417382-71417401 MS.gene071912:intron 65.0%
! AGGAGGGTGTGGAGGGTGGT+GGG - chr2.2:71417364-71417383 MS.gene071912:intron 65.0%
! GGTGAGGAGGAGGGTGTGGA+GGG - chr2.2:71417357-71417376 MS.gene071912:intron 65.0%
! TGGTGAGGAGGAGGGTGTGG+AGG - chr2.2:71417356-71417375 MS.gene071912:intron 65.0%
!! CGGCGGAAGTGGTAGTGGCA+TGG + chr2.2:71416846-71416865 None:intergenic 65.0%
!! GGTGGTGGGGTGGAAGGTGA+AGG - chr2.2:71417378-71417397 MS.gene071912:intron 65.0%
!! TGGGAGGGCGTTGAGGCAGT+TGG - chr2.2:71417267-71417286 MS.gene071912:intron 65.0%
GTTCGGAGGAGCGGCGGAAG+TGG + chr2.2:71416857-71416876 None:intergenic 70.0%
! GAGGAGGAGGGTGTGGAGGG+TGG - chr2.2:71417360-71417379 MS.gene071912:intron 70.0%
! GAGGAGGGTGTGGAGGGTGG+TGG - chr2.2:71417363-71417382 MS.gene071912:intron 70.0%
! GGAGGGTGTGGAGGGTGGTG+GGG - chr2.2:71417365-71417384 MS.gene071912:intron 70.0%
!! GTGGAGGGTGGTGGGGTGGA+AGG - chr2.2:71417372-71417391 MS.gene071912:intron 70.0%
!! GGGTGTGGAGGGTGGTGGGG+TGG - chr2.2:71417368-71417387 MS.gene071912:intron 75.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 71416806 71419193 71416806 ID=MS.gene071912
chr2.2 mRNA 71416806 71419193 71416806 ID=MS.gene071912.t1;Parent=MS.gene071912
chr2.2 exon 71418407 71419193 71418407 ID=MS.gene071912.t1.exon1;Parent=MS.gene071912.t1
chr2.2 CDS 71418407 71419193 71418407 ID=cds.MS.gene071912.t1;Parent=MS.gene071912.t1
chr2.2 exon 71416806 71417158 71416806 ID=MS.gene071912.t1.exon2;Parent=MS.gene071912.t1
chr2.2 CDS 71416806 71417158 71416806 ID=cds.MS.gene071912.t1;Parent=MS.gene071912.t1
Gene Sequence

>MS.gene071912

ATGGGGAAGAACGCGAAAAACACGGACGGCGGCGGCGCCATGCCACTACCACTTCCGCCGCTCCTCCGAACCCTAATCTCTTCCCTTTTCACCACCACCGCCGTTGACAAAACCACCACCTTCCTCTCCTTCTCTGTCAACTACAAACTCATCCAAACCCTCCGTTTCATCATCCTCACTTTCTACCTCTTCCTCCTCCGTTTCATTCCTTCTTTTTTCTTCAATGAAAACTACGAACACTCCACCATCAAAACGCAGAATAAGTTCACACACGATACACGAAACGACACTGCCATCGGACGTGCTCTTTCTCAGCTTCTTTCTGCATTAAACGATATTCCAGTTAGTTCAAGAAAATACGAAGCGGTTCGATCGTTAACGGAGAAGATTATCGATGATAATCACCTCGAAGGTGTTCATTCGTTACGTGAAGTTAATTGTGTTGCTTTGTCTTCAGCATTTGGGAGGGCGTTGAGGCAGTTGGAAGTGAAGGTGGCGGAGAGAGATCAAGGAGAGGGTGAAGAAGGTGATGGAGGAGAGTGGTATAGGATGGTGAGGAGGAGGGTGTGGAGGGTGGTGGGGTGGAAGGTGAAGGGAGGTGAGGGTGGTTTAAGTGGTGGTGTTCCGGCGGAGAAATTGGCAGCGGAGTTGGTTTGGATGGTGAAGAAGATGGTGGAGTGTGGTTGTGCTGATGAAGCACTTCGGAGATGGGCGGTGGCGGATAATTTAGGGTTTCTTGCTCTTACTGCTGATCCTCGTTTGCAGGCTTCGCTTGTTAAACTTGCAGGTACCATATATATCCATTCTTCATATTTTCTTATGTTATTATTTCAACTAGATGATTAACAATAATTTTACAATTTTGACCATGGTTTTAACTTTAAACTAATCATATATATTCAGCTAAATTAATCATAATTTTGCATGTAATTACCATCTTTATCAAAATTACTTGGCCGAGAATATAGGTGGTTTGAAAATATTAGCTCGAGAATGTGGACGTAGGTAGGTTGGAAAATTTCTGAGGCAAAGGAGGAAAGATGATAAGGTCGTGAATTGTTCAAACACTTTGTCAAAGCTGTTTGTGGTTGATATCATTCCTTGTAATGCAACAAGACAAAAATATTGTTGTCCAACTTGTAATTCTAGCAAGGGCACGTGTCTTGTTCTTATCATCTTTGAAAAATAAAGTAGCTGCATAAATAAATAATAAAGAAGCTGTTTTGTGAAATTCACTTTGATTTCAAAATTGCATGATAATAATAGTAGCTTTTGACACCAAAGGCAACGATTTGTCTTAAAATTAGCTATGAATTTAAGCCTTTGTACTCATTAGGCATTAGATTAGACTAGTTGTCCCGGGAAATTATATCCAAAGTTATACATACAATAAGAAAGAGTACAATTTAAATTTTATACTTTTTAAATCATTTAAAATGGTTGGCAGAAAGTTTATAGATTACAGCAAAAGATTTTTTAAATAGATATAGTGGCTGTCACAATGTTGAAATTCATTTAAAACATTGTTGTATCATTTTCAGTCACAGCAACATTCTGGCTTTGTCTTGTCTCCAAGTTTATAATGGCCAATAAACTTATTATGGGGCTTGAATCAATTTTCTATTCATCAAATAGAGATTAGTAGATTAGAAACATAAAAGAGATATAAGAGAAAATAAGTATGAGAAAACTAGAGTTGTTTTCCATTAGATTCTTGATCATAAATAACTTCCAAGGATTGTGGTCTCTCTGCACACCTAGAATGAAGAGTTACCTCTCATCCAATTTTTAGTTTATGCTTTGAAATGTATTTCTAGTTAAAGTGGGAGATTAAGATGACTCCAATACTTCATAGTGGTTCAAATTTAGAAGTGTTTAGGAAAATAAACTCAACACACTTCAAATTTCTTAATTTTCAGTAATTATTAATATTACAGCTCAATTGAATGACAATACAGAGTAGTTAAATTTATAGGCTAATCATCCTATGATATCTTTAATGTTCGCTAACAGTTGCTTTGAATTGTCCAAGCAGCATTTCTGTTCAAAGAAGCCAAAGATTTTGGCGCAGACGAAATTGAGGAAAGCAAGATGAAGCAATGCATGCAGGTAAAGTTAAAGATGTTACAGACATGGCTGCCATTGTTGTGCAGGGCCAGTAATGGCATTGATGCCCCGGCATTGAGCATCAATGAGAGGGCTGAGTTGGAGAGGATGTTGGAAAGCATTATAGAGGGGTTGGAACAAGAGAAGCAAGAACAGGTTCTTTCTCTTTGGCTCCACCATTTTACACATTGCTCATCCTCTGACTGGCCAAACCTCCAATCATGTTATGCTCGCTGGTGCTGTAAATCTCGCAAGCAGTTGCTGCTTTCGAGTGAAAATTGA
Protein sequence

>MS.gene071912.t1

MGKNAKNTDGGGAMPLPLPPLLRTLISSLFTTTAVDKTTTFLSFSVNYKLIQTLRFIILTFYLFLLRFIPSFFFNENYEHSTIKTQNKFTHDTRNDTAIGRALSQLLSALNDIPVSSRKYEAVRSLTEKIIDDNHLEGVHSLREVNCVALSSAFGRALRQLEVKVAERDQGEGEEGDGGEWYRMVRRRVWRVVGWKVKGGEGGLSGGVPAEKLAAELVWMVKKMVECGCADEALRRWAVADNLGFLALTADPRLQASLVKLAAFLFKEAKDFGADEIEESKMKQCMQVKLKMLQTWLPLLCRASNGIDAPALSINERAELERMLESIIEGLEQEKQEQVLSLWLHHFTHCSSSDWPNLQSCYARWCCKSRKQLLLSSEN