AlfalfaGEDB | MS.gene071981

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene071981.t1	AES63355.1	80.7	109	20	1	2	109	7	115	3.10E-36	161

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene071981.t1	G7IL67	80.7	109	20	1	2	109	7	115	2.3e-36	161.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene054892	MS.gene071981	0.804291	2.32E-49	-1.69E-46
MS.gene054893	MS.gene071981	0.800782	1.23E-48	-1.69E-46
MS.gene060412	MS.gene071981	0.80214	6.46E-49	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene071981.t1	MTR_2g007860	90.826	109	9	1	2	109	7	115	7.30e-67	197

Find 23 sgRNAs with CRISPR-Local

Find 25 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGTGACCTAATTCTCATTAA+AGG	0.176232	2.4:-75054529	None:intergenic
ACCTCCCTTTAATGAGAATT+AGG	0.311240	2.4:+75054524	MS.gene071981:CDS
TGCTATTACTAAGTTCTATT+AGG	0.322038	2.4:-75054278	None:intergenic
GTGACCTAATTCTCATTAAA+GGG	0.385524	2.4:-75054528	None:intergenic
ATCGAAGACGCAACAGAGTA+AGG	0.392483	2.4:+75054356	MS.gene071981:CDS
CGCTGCATCAATTTCCGTTA+GGG	0.412692	2.4:-75054250	None:intergenic
AAGGGAGGTAAAATAACTAT+AGG	0.423320	2.4:-75054510	None:intergenic
TCGCTGCATCAATTTCCGTT+AGG	0.435545	2.4:-75054251	None:intergenic
GGTTAAGGAGGAAGAATAAG+AGG	0.436235	2.4:+75054451	MS.gene071981:CDS
TAGTAATAGCACAGCCTACT+TGG	0.455809	2.4:+75054290	MS.gene071981:CDS
CGCTGTTGCTGCTATTGCTA+TGG	0.460075	2.4:-75054323	None:intergenic
AAATTGAAGATGAACGGTTA+AGG	0.495387	2.4:+75054436	MS.gene071981:CDS
CACATCCTCAACTGAATCTG+TGG	0.495772	2.4:-75054406	None:intergenic
ACTGAATCTGTGGGTGATGA+TGG	0.517018	2.4:-75054396	None:intergenic
AGATTCAGTTGAGGATGTGT+TGG	0.537928	2.4:+75054410	MS.gene071981:CDS
TTGAAGATGAACGGTTAAGG+AGG	0.572304	2.4:+75054439	MS.gene071981:CDS
TCGAAGACGCAACAGAGTAA+GGG	0.573379	2.4:+75054357	MS.gene071981:CDS
TGGCAGAAATTGAAGATGAA+CGG	0.589413	2.4:+75054430	MS.gene071981:CDS
CAATAGCAGCAACAGCGTCA+CGG	0.606320	2.4:+75054329	MS.gene071981:CDS
TATGTTGAAGAAGTGTATAG+AGG	0.618180	2.4:+75054480	MS.gene071981:CDS
ACATCCTCAACTGAATCTGT+GGG	0.636514	2.4:-75054405	None:intergenic
ACCTAATTCTCATTAAAGGG+AGG	0.645761	2.4:-75054525	None:intergenic
ATCACCCACAGATTCAGTTG+AGG	0.662122	2.4:+75054401	MS.gene071981:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	TGCTATTACTAAGTTCTATT+AGG	-	chr2.4:75054281-75054300	None:intergenic	25.0%
!!!	AAAACCATACTTTTTCTTCT+TGG	-	chr2.4:75054226-75054245	None:intergenic	25.0%
!!!	AAACCATACTTTTTCTTCTT+GGG	-	chr2.4:75054225-75054244	None:intergenic	25.0%
	AAATTGAAGATGAACGGTTA+AGG	+	chr2.4:75054436-75054455	MS.gene071981:CDS	30.0%
	AAGGGAGGTAAAATAACTAT+AGG	-	chr2.4:75054513-75054532	None:intergenic	30.0%
!	TATGTTGAAGAAGTGTATAG+AGG	+	chr2.4:75054480-75054499	MS.gene071981:CDS	30.0%
!!!	AACCATACTTTTTCTTCTTG+GGG	-	chr2.4:75054224-75054243	None:intergenic	30.0%
	AACCCCAAGAAGAAAAAGTA+TGG	+	chr2.4:75054219-75054238	MS.gene071981:CDS	35.0%
	TGGCAGAAATTGAAGATGAA+CGG	+	chr2.4:75054430-75054449	MS.gene071981:CDS	35.0%
!	ATGGCAATTTTCATCCAAGT+AGG	-	chr2.4:75054307-75054326	None:intergenic	35.0%
!!!	GTATGGTTTTTTCTCCCTAA+CGG	+	chr2.4:75054236-75054255	MS.gene071981:CDS	35.0%
	ACATCCTCAACTGAATCTGT+GGG	-	chr2.4:75054408-75054427	None:intergenic	40.0%
	AGATTCAGTTGAGGATGTGT+TGG	+	chr2.4:75054410-75054429	MS.gene071981:CDS	40.0%
	GGTTAAGGAGGAAGAATAAG+AGG	+	chr2.4:75054451-75054470	MS.gene071981:CDS	40.0%
	TAGTAATAGCACAGCCTACT+TGG	+	chr2.4:75054290-75054309	MS.gene071981:CDS	40.0%
	TTGAAGATGAACGGTTAAGG+AGG	+	chr2.4:75054439-75054458	MS.gene071981:CDS	40.0%
	ACTGAATCTGTGGGTGATGA+TGG	-	chr2.4:75054399-75054418	None:intergenic	45.0%
	ATCACCCACAGATTCAGTTG+AGG	+	chr2.4:75054401-75054420	MS.gene071981:CDS	45.0%
	ATCGAAGACGCAACAGAGTA+AGG	+	chr2.4:75054356-75054375	MS.gene071981:CDS	45.0%
	CACATCCTCAACTGAATCTG+TGG	-	chr2.4:75054409-75054428	None:intergenic	45.0%
	CGCTGCATCAATTTCCGTTA+GGG	-	chr2.4:75054253-75054272	None:intergenic	45.0%
	TCGAAGACGCAACAGAGTAA+GGG	+	chr2.4:75054357-75054376	MS.gene071981:CDS	45.0%
	TCGCTGCATCAATTTCCGTT+AGG	-	chr2.4:75054254-75054273	None:intergenic	45.0%
	CAATAGCAGCAACAGCGTCA+CGG	+	chr2.4:75054329-75054348	MS.gene071981:CDS	50.0%
	CGCTGTTGCTGCTATTGCTA+TGG	-	chr2.4:75054326-75054345	None:intergenic	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.4	gene	75054216	75054545	75054216	ID=MS.gene071981
chr2.4	mRNA	75054216	75054545	75054216	ID=MS.gene071981.t1;Parent=MS.gene071981
chr2.4	exon	75054216	75054545	75054216	ID=MS.gene071981.t1.exon1;Parent=MS.gene071981.t1
chr2.4	CDS	75054216	75054545	75054216	ID=cds.MS.gene071981.t1;Parent=MS.gene071981.t1

Gene Sequence	>MS.gene071981 ATGAACCCCAAGAAGAAAAAGTATGGTTTTTTCTCCCTAACGGAAATTGATGCAGCGAAACTCCTAATAGAACTTAGTAATAGCACAGCCTACTTGGATGAAAATTGCCATAGCAATAGCAGCAACAGCGTCACGGTGCAATCGAAGACGCAACAGAGTAAGGGAAGTGATGATATTTCACCATCATCACCCACAGATTCAGTTGAGGATGTGTTGGCAGAAATTGAAGATGAACGGTTAAGGAGGAAGAATAAGAGGTTTCGATATGTTGAAGAAGTGTATAGAGGTACAGATCCTATAGTTATTTTACCTCCCTTTAATGAGAATTAG
Protein sequence	>MS.gene071981.t1 MNPKKKKYGFFSLTEIDAAKLLIELSNSTAYLDENCHSNSSNSVTVQSKTQQSKGSDDISPSSPTDSVEDVLAEIEDERLRRKNKRFRYVEEVYRGTDPIVILPPFNEN

M. sativa cultivar XinJiangDaYe / MS.gene071981