AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene071993

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene071993.t1 MTR_2g007640 96.774 248 8 0 1 248 1 248 2.40e-179 492
MS.gene071993.t1 MTR_2g007640 96.889 225 7 0 1 225 1 225 1.81e-161 446
MS.gene071993.t1 MTR_2g007640 95.139 144 7 0 1 144 1 144 2.67e-97 282
MS.gene071993.t1 MTR_2g007300 63.000 200 71 2 49 248 47 243 2.95e-88 261
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene071993.t1 AT3G26070 57.971 207 81 4 46 248 38 242 5.45e-80 241
MS.gene071993.t1 AT3G26080 60.556 180 68 3 70 248 57 234 8.71e-72 219
MS.gene071993.t1 AT3G26080 52.153 209 68 4 70 248 57 263 2.47e-65 204

Find 59 sgRNAs with CRISPR-Local

Find 177 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTCATAGAGCTCAACTTTA+AGG 0.109395 2.4:+75212599 None:intergenic
CTGATTTGCTCAATGGAAAA+TGG 0.156678 2.4:-75212339 MS.gene071993:CDS
CGTATTTGGATGAAGATTTA+AGG 0.167214 2.4:-75210194 MS.gene071993:intron
TCAATTGGAAATTACGTATT+TGG 0.195162 2.4:-75210208 MS.gene071993:CDS
CCATTTGGTCGCAGGAATTT+TGG 0.196629 2.4:+75211139 None:intergenic
CTTCTTCAAAATTGCTAGTT+TGG 0.233095 2.4:-75210658 MS.gene071993:intron
CAACTTGTTTATCTTGAAAA+TGG 0.275931 2.4:-75209941 MS.gene071993:CDS
TTGAAGCACTCGCATCAAAT+AGG 0.280438 2.4:+75209898 None:intergenic
TATTGTACCTGGTTGTAAAA+TGG 0.296463 2.4:+75211058 None:intergenic
TGAGTTAAGTGGCACTAAAT+TGG 0.333704 2.4:+75210704 None:intergenic
ATGTTGGTGGTGTTGAATTT+TGG 0.338770 2.4:+75212724 None:intergenic
TAGCTGCTGATTCTGCAAAA+TGG 0.365601 2.4:-75212684 MS.gene071993:CDS
CAGATACCTATAAAATCATC+TGG 0.373103 2.4:-75210243 MS.gene071993:CDS
TGGGAGTGGTCGTGGTCAAT+TGG 0.414725 2.4:-75210223 MS.gene071993:CDS
TGGCAGAGTAGAAGTGGGAG+TGG 0.417431 2.4:+75212807 None:intergenic
TTCTGCAAAATGGAGGAACT+TGG 0.418599 2.4:-75212674 MS.gene071993:CDS
GCCTGGTAGATCTTTCCATT+TGG 0.421158 2.4:+75211124 None:intergenic
CTCAACTCTGATTTGCTCAA+TGG 0.427126 2.4:-75212346 MS.gene071993:CDS
TGAAGCACTCGCATCAAATA+GGG 0.432639 2.4:+75209899 None:intergenic
TCCACACGTTGTTGGTCCTC+AGG 0.437483 2.4:+75212541 None:intergenic
CCAAAATTCCTGCGACCAAA+TGG 0.438668 2.4:-75211139 MS.gene071993:CDS
TAACCTTGTCCACACGTTGT+TGG 0.440005 2.4:+75212533 None:intergenic
TGATTTGCTCAATGGAAAAT+GGG 0.442608 2.4:-75212338 MS.gene071993:CDS
TGAGGCTATGAACTCTGTTA+AGG 0.456748 2.4:-75212374 MS.gene071993:CDS
GCTACCCTTCTTGAGTTAAG+TGG 0.467062 2.4:+75210693 None:intergenic
GAAGATTTGGTTGTGAAGGA+TGG 0.468096 2.4:+75212764 None:intergenic
TAGTGCCACTTAACTCAAGA+AGG 0.474795 2.4:-75210698 MS.gene071993:CDS
GAATCAATTATACATTTGAA+TGG 0.483996 2.4:+75212787 None:intergenic
ATGTATCTGTATTAATTGCC+TGG 0.490157 2.4:+75211107 None:intergenic
AGAATATCAAGAGGTAATAG+AGG 0.493006 2.4:-75209964 MS.gene071993:CDS
GAATCAGCAGCTACTGATGA+TGG 0.500537 2.4:+75212694 None:intergenic
GCATCAAATAGGGACTCTAT+AGG 0.504016 2.4:+75209909 None:intergenic
AGATCTTTCCATTTGGTCGC+AGG 0.508870 2.4:+75211131 None:intergenic
TTCCTCCAGTTAGAAAACCT+TGG 0.517994 2.4:+75212641 None:intergenic
AAGATTTGGTTGTGAAGGAT+GGG 0.520551 2.4:+75212765 None:intergenic
AAATCATCTGGGAGTGGTCG+TGG 0.522383 2.4:-75210231 MS.gene071993:CDS
GAGCTCAAAATATAGAAACT+TGG 0.545026 2.4:-75211081 MS.gene071993:CDS
TTTGAATGGCAGAGTAGAAG+TGG 0.547151 2.4:+75212801 None:intergenic
AACTTGGTTTCAATCTTCCA+AGG 0.553044 2.4:-75212658 MS.gene071993:CDS
CTAGATTGCTAGTAAACTTG+AGG 0.574347 2.4:-75212392 MS.gene071993:intron
CAAATAGGGACTCTATAGGA+TGG 0.578281 2.4:+75209913 None:intergenic
TCCTGAGGACCAACAACGTG+TGG 0.586912 2.4:-75212542 MS.gene071993:CDS
CCTATAAAATCATCTGGGAG+TGG 0.588827 2.4:-75210237 MS.gene071993:CDS
AAGTGGCACTAAATTGGCAG+TGG 0.602208 2.4:+75210710 None:intergenic
GGACCAACAACGTGTGGACA+AGG 0.602478 2.4:-75212536 MS.gene071993:intron
TGATGATGGTAATATGATGT+TGG 0.604952 2.4:+75212708 None:intergenic
GCTTCAGCACCACGTTCAAG+TGG 0.605538 2.4:+75212568 None:intergenic
AAAGGAAGATTTGGTTGTGA+AGG 0.609629 2.4:+75212760 None:intergenic
TGATGGTAATATGATGTTGG+TGG 0.609634 2.4:+75212711 None:intergenic
TCTAACTGGAGGAAGAGGAA+AGG 0.618212 2.4:-75212632 MS.gene071993:CDS
AGTGCCACTTAACTCAAGAA+GGG 0.619471 2.4:-75210697 MS.gene071993:CDS
AGATACCTATAAAATCATCT+GGG 0.643096 2.4:-75210242 MS.gene071993:CDS
CTGCTGATTCTGCAAAATGG+AGG 0.648936 2.4:-75212681 MS.gene071993:CDS
GAACCTGACAGAATATCAAG+AGG 0.650729 2.4:-75209973 MS.gene071993:intron
TTGAATGGCAGAGTAGAAGT+GGG 0.655278 2.4:+75212802 None:intergenic
TTGAGCAAATCAGAGTTGAG+AGG 0.664638 2.4:+75212349 None:intergenic
ACCAAATGGAAAGATCTACC+AGG 0.666458 2.4:-75211125 MS.gene071993:CDS
TGGTGCTGAAGCTACTCCTG+AGG 0.671485 2.4:-75212557 MS.gene071993:CDS
ACAATTGAGCCACTTGAACG+TGG 0.739802 2.4:-75212577 MS.gene071993:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAATGATATTCTTTAATTTA+AGG - chr2.4:75210808-75210827 MS.gene071993:intron 10.0%
!! ATTAAAAGAAAAAATGTAAG+TGG + chr2.4:75211040-75211059 None:intergenic 15.0%
!!! AAAATTTAATGATTTAGAGT+TGG - chr2.4:75211369-75211388 MS.gene071993:intron 15.0%
!!! AAATTTAATGATTTAGAGTT+GGG - chr2.4:75211370-75211389 MS.gene071993:intron 15.0%
!!! ATTATTGAAAAGCTTTAAAA+CGG + chr2.4:75211943-75211962 None:intergenic 15.0%
!!! TAGCTATTTTAAATGTATTA+TGG - chr2.4:75210906-75210925 MS.gene071993:intron 15.0%
!!! TCTTATTTATGACTTTTATA+TGG - chr2.4:75210551-75210570 MS.gene071993:intron 15.0%
!! AAAAAAAAAAAACATCTTGC+AGG + chr2.4:75211113-75211132 None:intergenic 20.0%
!! AATTAATCATCACTTTACAA+GGG + chr2.4:75211564-75211583 None:intergenic 20.0%
!! ATCAAAATCGATAAAAGATA+TGG + chr2.4:75210748-75210767 None:intergenic 20.0%
!! CATAAATAAGAACCTATAAT+TGG + chr2.4:75210542-75210561 None:intergenic 20.0%
!! GAAAATTGAAAAGTTAATAC+TGG + chr2.4:75212121-75212140 None:intergenic 20.0%
!! GAATCAATTATACATTTGAA+TGG + chr2.4:75209960-75209979 None:intergenic 20.0%
!!! AAATAGCTAAATCAACATTT+TGG + chr2.4:75210895-75210914 None:intergenic 20.0%
!!! ATATCTTTTATCGATTTTGA+TGG - chr2.4:75210747-75210766 MS.gene071993:intron 20.0%
!!! ATTGATATTTTTCAATCCAA+GGG + chr2.4:75210627-75210646 None:intergenic 20.0%
!!! TTCACTAAAGATTTTTTTGA+TGG - chr2.4:75210773-75210792 MS.gene071993:intron 20.0%
! AAACAGATACTTAAACCAAT+GGG + chr2.4:75211718-75211737 None:intergenic 25.0%
! AAAGAAAAAATGTAAGTGGT+CGG + chr2.4:75211036-75211055 None:intergenic 25.0%
! AAATGTATTATGGATTCAGT+TGG - chr2.4:75210916-75210935 MS.gene071993:intron 25.0%
! AAGAACCTATAATTGGAATT+TGG + chr2.4:75210535-75210554 None:intergenic 25.0%
! AATCCATGTAGTTGAAATAT+TGG - chr2.4:75211748-75211767 MS.gene071993:intron 25.0%
! AATTTCAGAGATTAAATTGC+TGG - chr2.4:75210850-75210869 MS.gene071993:intron 25.0%
! AGATACCTATAAAATCATCT+GGG - chr2.4:75212502-75212521 MS.gene071993:intron 25.0%
! ATAGATAGAGCTTATAAAGA+TGG + chr2.4:75211210-75211229 None:intergenic 25.0%
! CAATTAATCATCACTTTACA+AGG + chr2.4:75211565-75211584 None:intergenic 25.0%
! CATAAAATCAATCACAAACT+AGG + chr2.4:75210322-75210341 None:intergenic 25.0%
! GATATCCAAATTCCAATTAT+AGG - chr2.4:75210527-75210546 MS.gene071993:intron 25.0%
! GTGCTATAAAATATTAAGTC+TGG - chr2.4:75212343-75212362 MS.gene071993:CDS 25.0%
! TAAACAGATACTTAAACCAA+TGG + chr2.4:75211719-75211738 None:intergenic 25.0%
! TAAAGCATATGTGCTTATAT+TGG - chr2.4:75210687-75210706 MS.gene071993:CDS 25.0%
! TAGATAGAGCTTATAAAGAT+GGG + chr2.4:75211209-75211228 None:intergenic 25.0%
! TCAATTGGAAATTACGTATT+TGG - chr2.4:75212536-75212555 MS.gene071993:intron 25.0%
! TGTTACATGCATAATAGTTT+AGG - chr2.4:75210261-75210280 MS.gene071993:intron 25.0%
! TTTCCAATATTTCAACTACA+TGG + chr2.4:75211754-75211773 None:intergenic 25.0%
!! AAAACAGACTCTTAAGTTTT+CGG - chr2.4:75211063-75211082 MS.gene071993:intron 25.0%
!! CAACTTGTTTATCTTGAAAA+TGG - chr2.4:75212803-75212822 MS.gene071993:CDS 25.0%
!! CATAATAGTTTAGGTTTATG+TGG - chr2.4:75210270-75210289 MS.gene071993:intron 25.0%
!! TAAATTTTCCATAAGTATGC+TGG + chr2.4:75211357-75211376 None:intergenic 25.0%
!! TCAGGAAAACATATTTTTCA+GGG + chr2.4:75212457-75212476 None:intergenic 25.0%
!!! AAACATATTTTTCAGGGATT+TGG + chr2.4:75212451-75212470 None:intergenic 25.0%
!!! AGTTTGTGATTGATTTTATG+TGG - chr2.4:75210322-75210341 MS.gene071993:intron 25.0%
!!! CATTGATATTTTTCAATCCA+AGG + chr2.4:75210628-75210647 None:intergenic 25.0%
AACTATATTGCATTAGGATC+TGG + chr2.4:75210502-75210521 None:intergenic 30.0%
ACTTCAAAATCAGCAGTATA+TGG - chr2.4:75212652-75212671 MS.gene071993:CDS 30.0%
AGAATATCAAGAGGTAATAG+AGG - chr2.4:75212780-75212799 MS.gene071993:CDS 30.0%
ATATGCACATATAAGTGATC+AGG - chr2.4:75212703-75212722 MS.gene071993:CDS 30.0%
ATATTCATACTCAATCAGCA+TGG + chr2.4:75212738-75212757 None:intergenic 30.0%
ATGTATCTGTATTAATTGCC+TGG + chr2.4:75211640-75211659 None:intergenic 30.0%
ATTTGATGAAGTTAGCATGT+AGG + chr2.4:75211971-75211990 None:intergenic 30.0%
CAACAACAGTGAAAATTTAC+GGG + chr2.4:75212010-75212029 None:intergenic 30.0%
CAGAGAAACTATATTGCATT+AGG + chr2.4:75210508-75210527 None:intergenic 30.0%
CAGATACCTATAAAATCATC+TGG - chr2.4:75212501-75212520 MS.gene071993:intron 30.0%
CGTATTTGGATGAAGATTTA+AGG - chr2.4:75212550-75212569 MS.gene071993:CDS 30.0%
CTTCTTCAAAATTGCTAGTT+TGG - chr2.4:75212086-75212105 MS.gene071993:intron 30.0%
GAGCTCAAAATATAGAAACT+TGG - chr2.4:75211663-75211682 MS.gene071993:intron 30.0%
GCAACAACAGTGAAAATTTA+CGG + chr2.4:75212011-75212030 None:intergenic 30.0%
GTTAAAAGACGATGACAAAT+AGG + chr2.4:75212152-75212171 None:intergenic 30.0%
TAGAAGAATTCAAGAAACCT+TGG - chr2.4:75211805-75211824 MS.gene071993:intron 30.0%
TATAAAACTACTCCTTTGTG+TGG - chr2.4:75211269-75211288 MS.gene071993:intron 30.0%
TATGCACATATAAGTGATCA+GGG - chr2.4:75212704-75212723 MS.gene071993:CDS 30.0%
TATTGTACCTGGTTGTAAAA+TGG + chr2.4:75211689-75211708 None:intergenic 30.0%
TTAACATGCTCATCAAAATG+TGG - chr2.4:75210978-75210997 MS.gene071993:intron 30.0%
TTTCATAGAGCTCAACTTTA+AGG + chr2.4:75210148-75210167 None:intergenic 30.0%
! AAAATATTAAGTCTGGAACC+AGG - chr2.4:75212350-75212369 MS.gene071993:CDS 30.0%
! AAGGGTCAAATTGTCTATTT+TGG - chr2.4:75211296-75211315 MS.gene071993:intron 30.0%
! GTCAGGAAAACATATTTTTC+AGG + chr2.4:75212458-75212477 None:intergenic 30.0%
! TAAATTTTCACTGTTGTTGC+AGG - chr2.4:75212011-75212030 MS.gene071993:intron 30.0%
! TCAATCAATTTTGCAAACAC+AGG - chr2.4:75210448-75210467 MS.gene071993:intron 30.0%
!! GCACATATGCTTTAAAATTG+AGG + chr2.4:75210681-75210700 None:intergenic 30.0%
!! GCATTTGAGTTGTTAATTAC+TGG - chr2.4:75212182-75212201 MS.gene071993:intron 30.0%
!! TGATGATGGTAATATGATGT+TGG + chr2.4:75210039-75210058 None:intergenic 30.0%
!! TGATTTGCTCAATGGAAAAT+GGG - chr2.4:75210406-75210425 MS.gene071993:intron 30.0%
!! TTTGTTTCTGGAATTCAACA+TGG - chr2.4:75212624-75212643 MS.gene071993:CDS 30.0%
AACTTGGTTTCAATCTTCCA+AGG - chr2.4:75210086-75210105 MS.gene071993:intron 35.0%
ATCACAAATCAACACATTCG+CGG - chr2.4:75212251-75212270 MS.gene071993:intron 35.0%
ATCCAAGGGACTAAATTGAT+AGG + chr2.4:75210613-75210632 None:intergenic 35.0%
ATGACTTACCAGCATACTTA+TGG - chr2.4:75211346-75211365 MS.gene071993:intron 35.0%
CTAGATTGCTAGTAAACTTG+AGG - chr2.4:75210352-75210371 MS.gene071993:intron 35.0%
GATAGGCACTTACAATTTCA+AGG + chr2.4:75210596-75210615 None:intergenic 35.0%
GATTAAAGATCAGATAGTCC+TGG + chr2.4:75212371-75212390 None:intergenic 35.0%
GCATTGTACACTTATTTGTC+AGG + chr2.4:75212475-75212494 None:intergenic 35.0%
TGAGTTAAGTGGCACTAAAT+TGG + chr2.4:75212043-75212062 None:intergenic 35.0%
TGATGGTAATATGATGTTGG+TGG + chr2.4:75210036-75210055 None:intergenic 35.0%
TTACCTCTTGATATTCTGTC+AGG + chr2.4:75212777-75212796 None:intergenic 35.0%
TTTGGCACTCGAAATTGTAA+GGG + chr2.4:75210877-75210896 None:intergenic 35.0%
! AAAGGAAGATTTGGTTGTGA+AGG + chr2.4:75209987-75210006 None:intergenic 35.0%
! AAGATTTGGTTGTGAAGGAT+GGG + chr2.4:75209982-75210001 None:intergenic 35.0%
! ATCTTCCAAGGTTTTCTAAC+TGG - chr2.4:75210098-75210117 MS.gene071993:intron 35.0%
! CAGGTCTATTTTACGAGATT+GGG + chr2.4:75211094-75211113 None:intergenic 35.0%
! CATTGTTACTTACATTTGCG+TGG + chr2.4:75211893-75211912 None:intergenic 35.0%
! GAATCAAACAGGGTTCTTTT+AGG - chr2.4:75211166-75211185 MS.gene071993:intron 35.0%
! GATGAAGATTTAAGGTGAGT+TGG - chr2.4:75212558-75212577 MS.gene071993:CDS 35.0%
! TCAATTTTGCAAACACAGGT+TGG - chr2.4:75210452-75210471 MS.gene071993:intron 35.0%
! TTCTTTTAGGCTAACAGATG+TGG - chr2.4:75211179-75211198 MS.gene071993:intron 35.0%
! TTTTGGCACTCGAAATTGTA+AGG + chr2.4:75210878-75210897 None:intergenic 35.0%
!! CTGATTTGCTCAATGGAAAA+TGG - chr2.4:75210405-75210424 MS.gene071993:intron 35.0%
!!! ATGTTGGTGGTGTTGAATTT+TGG + chr2.4:75210023-75210042 None:intergenic 35.0%
AAAGAACCCTGTTTGATTCC+TGG + chr2.4:75211165-75211184 None:intergenic 40.0%
AAGAACCCTGTTTGATTCCT+GGG + chr2.4:75211164-75211183 None:intergenic 40.0%
ACCAAATGGAAAGATCTACC+AGG - chr2.4:75211619-75211638 MS.gene071993:intron 40.0%
ACTCCTTTGTGTGGTCAAAA+GGG - chr2.4:75211278-75211297 MS.gene071993:intron 40.0%
CAAATAGGGACTCTATAGGA+TGG + chr2.4:75212834-75212853 None:intergenic 40.0%
CCTATAAAATCATCTGGGAG+TGG - chr2.4:75212507-75212526 MS.gene071993:intron 40.0%
CTGTCTTGCTCATTATTGAC+AGG + chr2.4:75211408-75211427 None:intergenic 40.0%
CTTGATATTCTGTCAGGTTC+AGG + chr2.4:75212771-75212790 None:intergenic 40.0%
GAACCTGACAGAATATCAAG+AGG - chr2.4:75212771-75212790 MS.gene071993:CDS 40.0%
GATCACAGCTCATTTGTTTC+TGG - chr2.4:75212612-75212631 MS.gene071993:CDS 40.0%
GGTCGGTCTACATTCAATAT+GGG + chr2.4:75211019-75211038 None:intergenic 40.0%
TACTCCTTTGTGTGGTCAAA+AGG - chr2.4:75211277-75211296 MS.gene071993:intron 40.0%
TGAGGCTATGAACTCTGTTA+AGG - chr2.4:75210370-75210389 MS.gene071993:intron 40.0%
TGCCTATCAATTTAGTCCCT+TGG - chr2.4:75210608-75210627 MS.gene071993:intron 40.0%
TGGTCGGTCTACATTCAATA+TGG + chr2.4:75211020-75211039 None:intergenic 40.0%
TTCCTCCAGTTAGAAAACCT+TGG + chr2.4:75210106-75210125 None:intergenic 40.0%
TTCTGCAAAATGGAGGAACT+TGG - chr2.4:75210070-75210089 MS.gene071993:intron 40.0%
TTGAATGGCAGAGTAGAAGT+GGG + chr2.4:75209945-75209964 None:intergenic 40.0%
TTGAGCAAATCAGAGTTGAG+AGG + chr2.4:75210398-75210417 None:intergenic 40.0%
TTTGAATGGCAGAGTAGAAG+TGG + chr2.4:75209946-75209965 None:intergenic 40.0%
! ATGGTTTGTGTCATGGAGAA+TGG - chr2.4:75212212-75212231 MS.gene071993:intron 40.0%
! CCACTCCCAGATGATTTTAT+AGG + chr2.4:75212510-75212529 None:intergenic 40.0%
! CTCAAGTCTCACAGTGATTT+TGG + chr2.4:75211234-75211253 None:intergenic 40.0%
! GAAGATTTGGTTGTGAAGGA+TGG + chr2.4:75209983-75210002 None:intergenic 40.0%
! GCAGGTCTATTTTACGAGAT+TGG + chr2.4:75211095-75211114 None:intergenic 40.0%
! GTTAATTACTGGCTACGTCA+TGG - chr2.4:75212193-75212212 MS.gene071993:intron 40.0%
! TGTGTCATGGAGAATGGATT+TGG - chr2.4:75212218-75212237 MS.gene071993:intron 40.0%
! TTCCAAGGTTTTCTAACTGG+AGG - chr2.4:75210101-75210120 MS.gene071993:intron 40.0%
!! AACTTGGCCATTTTACAACC+AGG - chr2.4:75211679-75211698 MS.gene071993:intron 40.0%
!! AGTGCCACTTAACTCAAGAA+GGG - chr2.4:75212047-75212066 MS.gene071993:intron 40.0%
!! CTCAACTCTGATTTGCTCAA+TGG - chr2.4:75210398-75210417 MS.gene071993:intron 40.0%
!! TAGCTGCTGATTCTGCAAAA+TGG - chr2.4:75210060-75210079 MS.gene071993:intron 40.0%
!! TAGTGCCACTTAACTCAAGA+AGG - chr2.4:75212046-75212065 MS.gene071993:intron 40.0%
!! TCAAGAAACCTTGGTGTTGA+CGG - chr2.4:75211814-75211833 MS.gene071993:intron 40.0%
!! TGGTGTTGAATTTTGGTCGT+GGG + chr2.4:75210016-75210035 None:intergenic 40.0%
AAGTGGCACTAAATTGGCAG+TGG + chr2.4:75212037-75212056 None:intergenic 45.0%
ACAATTGAGCCACTTGAACG+TGG - chr2.4:75210167-75210186 MS.gene071993:intron 45.0%
ACTAAAACCCGTCAACACCA+AGG + chr2.4:75211825-75211844 None:intergenic 45.0%
AGATCTTTCCATTTGGTCGC+AGG + chr2.4:75211616-75211635 None:intergenic 45.0%
ATCTCACACATTCACGCAGT+CGG + chr2.4:75212411-75212430 None:intergenic 45.0%
ATGGGCAGAACTATTGTACC+TGG + chr2.4:75211700-75211719 None:intergenic 45.0%
CATTCGCGGAGTTAACACAA+TGG - chr2.4:75212265-75212284 MS.gene071993:intron 45.0%
CCAAAATTCCTGCGACCAAA+TGG - chr2.4:75211605-75211624 MS.gene071993:intron 45.0%
GAATCAGCAGCTACTGATGA+TGG + chr2.4:75210053-75210072 None:intergenic 45.0%
GAGCTGTGATCATGTGAGAA+GGG + chr2.4:75212603-75212622 None:intergenic 45.0%
GCCTGGTAGATCTTTCCATT+TGG + chr2.4:75211623-75211642 None:intergenic 45.0%
GCTACCCTTCTTGAGTTAAG+TGG + chr2.4:75212054-75212073 None:intergenic 45.0%
TAACCTTGTCCACACGTTGT+TGG + chr2.4:75210214-75210233 None:intergenic 45.0%
TCTAACTGGAGGAAGAGGAA+AGG - chr2.4:75210112-75210131 MS.gene071993:intron 45.0%
TGAGCTGTGATCATGTGAGA+AGG + chr2.4:75212604-75212623 None:intergenic 45.0%
! CCATTTGGTCGCAGGAATTT+TGG + chr2.4:75211608-75211627 None:intergenic 45.0%
! CTACGTCATGGTTTGTGTCA+TGG - chr2.4:75212205-75212224 MS.gene071993:intron 45.0%
! GGTACAATAGTTCTGCCCAT+TGG - chr2.4:75211700-75211719 MS.gene071993:intron 45.0%
! GGTTTTCTAACTGGAGGAAG+AGG - chr2.4:75210107-75210126 MS.gene071993:intron 45.0%
! TGACCCTTTTGACCACACAA+AGG + chr2.4:75211284-75211303 None:intergenic 45.0%
!! ACGGGTTTTAGTGTCGCAAT+AGG - chr2.4:75211833-75211852 MS.gene071993:intron 45.0%
!! CAAGAAACCTTGGTGTTGAC+GGG - chr2.4:75211815-75211834 MS.gene071993:intron 45.0%
!! CAGGTTGGTTTCTAGAGTAG+AGG - chr2.4:75210467-75210486 MS.gene071993:intron 45.0%
!! CTGCTGATTCTGCAAAATGG+AGG - chr2.4:75210063-75210082 MS.gene071993:intron 45.0%
!! GGTGTTGAATTTTGGTCGTG+GGG + chr2.4:75210015-75210034 None:intergenic 45.0%
!! GTGGTGTTGAATTTTGGTCG+TGG + chr2.4:75210017-75210036 None:intergenic 45.0%
!! GTGTTGAATTTTGGTCGTGG+GGG + chr2.4:75210014-75210033 None:intergenic 45.0%
!! TCGCAGGAATTTTGGTCTCT+AGG + chr2.4:75211600-75211619 None:intergenic 45.0%
AAAGTGATAACAGCCAGCCC+AGG - chr2.4:75211144-75211163 MS.gene071993:CDS 50.0%
AAATCATCTGGGAGTGGTCG+TGG - chr2.4:75212513-75212532 MS.gene071993:intron 50.0%
TCACGCAGTCGGCATATTGA+TGG + chr2.4:75212400-75212419 None:intergenic 50.0%
! GGGGGTGCAAAAGGAAGATT+TGG + chr2.4:75209996-75210015 None:intergenic 50.0%
! GTCGGCATATTGATGGCAGA+TGG + chr2.4:75212393-75212412 None:intergenic 50.0%
ACCCTGTTTGATTCCTGGGC+TGG + chr2.4:75211160-75211179 None:intergenic 55.0%
AGCCAGCCCAGGAATCAAAC+AGG - chr2.4:75211155-75211174 MS.gene071993:intron 55.0%
GCCAGCCCAGGAATCAAACA+GGG - chr2.4:75211156-75211175 MS.gene071993:intron 55.0%
GCTTCAGCACCACGTTCAAG+TGG + chr2.4:75210179-75210198 None:intergenic 55.0%
GGACCAACAACGTGTGGACA+AGG - chr2.4:75210208-75210227 MS.gene071993:CDS 55.0%
TCCACACGTTGTTGGTCCTC+AGG + chr2.4:75210206-75210225 None:intergenic 55.0%
TCCTGAGGACCAACAACGTG+TGG - chr2.4:75210202-75210221 MS.gene071993:CDS 55.0%
TGGCAGAGTAGAAGTGGGAG+TGG + chr2.4:75209940-75209959 None:intergenic 55.0%
TGGGAGTGGTCGTGGTCAAT+TGG - chr2.4:75212521-75212540 MS.gene071993:intron 55.0%
! TGGTGCTGAAGCTACTCCTG+AGG - chr2.4:75210187-75210206 MS.gene071993:intron 55.0%
! TTTGGTCGTGGGGGTGCAAA+AGG + chr2.4:75210005-75210024 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 75209912 75212854 75209912 ID=MS.gene071993
chr2.4 mRNA 75209912 75212854 75209912 ID=MS.gene071993.t1;Parent=MS.gene071993
chr2.4 exon 75212537 75212854 75212537 ID=MS.gene071993.t1.exon1;Parent=MS.gene071993.t1
chr2.4 CDS 75212537 75212854 75212537 ID=cds.MS.gene071993.t1;Parent=MS.gene071993.t1
chr2.4 exon 75212297 75212410 75212297 ID=MS.gene071993.t1.exon2;Parent=MS.gene071993.t1
chr2.4 CDS 75212297 75212410 75212297 ID=cds.MS.gene071993.t1;Parent=MS.gene071993.t1
chr2.4 exon 75211066 75211164 75211066 ID=MS.gene071993.t1.exon3;Parent=MS.gene071993.t1
chr2.4 CDS 75211066 75211164 75211066 ID=cds.MS.gene071993.t1;Parent=MS.gene071993.t1
chr2.4 exon 75210659 75210733 75210659 ID=MS.gene071993.t1.exon4;Parent=MS.gene071993.t1
chr2.4 CDS 75210659 75210733 75210659 ID=cds.MS.gene071993.t1;Parent=MS.gene071993.t1
chr2.4 exon 75210195 75210262 75210195 ID=MS.gene071993.t1.exon5;Parent=MS.gene071993.t1
chr2.4 CDS 75210195 75210262 75210195 ID=cds.MS.gene071993.t1;Parent=MS.gene071993.t1
chr2.4 exon 75209912 75209984 75209912 ID=MS.gene071993.t1.exon6;Parent=MS.gene071993.t1
chr2.4 CDS 75209912 75209984 75209912 ID=cds.MS.gene071993.t1;Parent=MS.gene071993.t1
Gene Sequence

>MS.gene071993

ATGACTTATCTCTCAGCAGTTCTCTCCACTCCCACTTCTACTCTGCCATTCAAATGTATAATTGATTCCCATCCTTCACAACCAAATCTTCCTTTTGCACCCCCACGACCAAAATTCAACACCACCAACATCATATTACCATCATCAGTAGCTGCTGATTCTGCAAAATGGAGGAACTTGGTTTCAATCTTCCAAGGTTTTCTAACTGGAGGAAGAGGAAAGGATGTTCAGAGCCTTAAAGTTGAGCTCTATGAAACAATTGAGCCACTTGAACGTGGTGCTGAAGCTACTCCTGAGGACCAACAACGTGTGGACAAGGTTAAAGTTATTAAAAGTACAAACAGTAACATGTTACATGCATAATAGTTTAGGTTTATGTGGCTTTGTTTTATTGTATTACTCTGTTTCCTAGTTTGTGATTGATTTTATGTGGATCTTGTCTAGATTGCTAGTAAACTTGAGGCTATGAACTCTGTTAAGGAGCCTCTCAACTCTGATTTGCTCAATGGAAAATGGGAGCTTTTATATACAACATCTCAATCAATTTTGCAAACACAGGTTGGTTTCTAGAGTAGAGGTTTATAACTCCAGATCCTAATGCAATATAGTTTCTCTGATATCCAAATTCCAATTATAGGTTCTTATTTATGACTTTTATATGGAGTAAATAAACATTTCAGTCCTTGAAATTGTAAGTGCCTATCAATTTAGTCCCTTGGATTGAAAAATATCAATGAATTTAGTATTTTTTTCTTCAAAATTCGCACCTCAATTTTAAAGCATATGTGCTTATATTGGATGCAACAAAAGACTATTTAAGTCGATATTTAGTACCATATCTTTTATCGATTTTGATGGATGTTCACTAAAGATTTTTTTGATGGAGAGATTAAATTAAATGATATTCTTTAATTTAAGGACTAATTTGCTATTTTGTAAATTTCAGAGATTAAATTGCTGGACCCTTACAATTTCGAGTGCCAAAATGTTGATTTAGCTATTTTAAATGTATTATGGATTCAGTTGGCACGTATCATAAGTCATAACTGATCTGTTTGCAGTTTAATTAACATGCTCATCAAAATGTGGCAATTAGCTGTACTTCCCATATTGAATGTAGACCGACCACTTACATTTTTTCTTTTAATAACAAAACAGACTCTTAAGTTTTCGGTTTAGCCCAATCTCGTAAAATAGACCTGCAAGATGTTTTTTTTTTTTTTTTTGAATAAAAAGTGATAACAGCCAGCCCAGGAATCAAACAGGGTTCTTTTAGGCTAACAGATGTGGATTGCCCATCTTTATAAGCTCTATCTATGCCAAAATCACTGTGAGACTTGAGTTTTTTTTTTCTCAGTATAAAACTACTCCTTTGTGTGGTCAAAAGGGTCAAATTGTCTATTTTGGTTACTCTTATTGACAGAAACTAATTTGATGACTTACCAGCATACTTATGGAAAATTTAATGATTTAGAGTTGGGCATAGATTGAGTCCTGTCAATAATGAGCAAGACAGTTTTGCTTGACTTACATTAGTTTATCAGTTGATCTTGATTCAAACAAGAATTGCACGTTCTAATTCTCTTTTTGTTGTTTTCATTATAACATGATCAATTTTGCGATGTTGTTTTTATATTTATATTCATTTGCCCTTGTAAAGTGATGATTAATTGATTGTAAACTGTCCTAGAGACCAAAATTCCTGCGACCAAATGGAAAGATCTACCAGGCAATTAATACAGATACATTGAGAGCTCAAAATATAGAAACTTGGCCATTTTACAACCAGGTACAATAGTTCTGCCCATTGGTTTAAGTATCTGTTTACATTGTGTAAATCCATGTAGTTGAAATATTGGAAATAAAAATCTTGCTTTGATATGAGCTGTTCAATAGAAGAATTCAAGAAACCTTGGTGTTGACGGGTTTTAGTGTCGCAATAGGAACTTATGCTAATATGATTTTGAGATGAACATGACCACGCAAATGTAAGTAACAATGTCTTGTTGTAACATGATGTGTTGAAGACCGTTTTAAAGCTTTTCAATAATAAGTTCCTACATGCTAACTTCATCAAATTCATAATGTACTGTAGCCCGTAAATTTTCACTGTTGTTGCAGGCCACTGCCAATTTAGTGCCACTTAACTCAAGAAGGGTAGCTGTCAAGTTTGACTTCTTCAAAATTGCTAGTTTGGTATGTACTGCCAGTATTAACTTTTCAATTTTCATATTTAGCCTATTTGTCATCGTCTTTTAACATACGATCATGCATTTGAGTTGTTAATTACTGGCTACGTCATGGTTTGTGTCATGGAGAATGGATTTGGATTATATCTAATCACAAATCAACACATTCGCGGAGTTAACACAATGGTGACTGTTGTATTGAAATCTAACAGTACAAAAAACATGTAGATTTTATTTTTCTTGTGCTATAAAATATTAAGTCTGGAACCAGGACTATCTGATCTTTAATCCATCTGCCATCAATATGCCGACTGCGTGAATGTGTGAGATTTGCGACTGCATACAGTCCAAATCCCTGAAAAATATGTTTTCCTGACAAATAAGTGTACAATGCTAATTACAGATACCTATAAAATCATCTGGGAGTGGTCGTGGTCAATTGGAAATTACGTATTTGGATGAAGATTTAAGGTGAGTTGGTACTAATAGAATTAATTGTCCCTTCTCACATGATCACAGCTCATTTGTTTCTGGAATTCAACATGGTTTGCACTTCAAAATCAGCAGTATATGGTTAAATACTAGTTTGTCAAACATAACATATATGCACATATAAGTGATCAGGGTTTGAATTCCATGCTGATTGAGTATGAATATCTTTCTTTTGCCTGAACCTGACAGAATATCAAGAGGTAATAGAGGCAACTTGTTTATCTTGAAAATGGTAGATCCATCCTATAGAGTCCCTATTTGA
Protein sequence

>MS.gene071993.t1

MTYLSAVLSTPTSTLPFKCIIDSHPSQPNLPFAPPRPKFNTTNIILPSSVAADSAKWRNLVSIFQGFLTGGRGKDVQSLKVELYETIEPLERGAEATPEDQQRVDKIASKLEAMNSVKEPLNSDLLNGKWELLYTTSQSILQTQRPKFLRPNGKIYQAINTDTLRAQNIETWPFYNQATANLVPLNSRRVAVKFDFFKIASLIPIKSSGSGRGQLEITYLDEDLRISRGNRGNLFILKMVDPSYRVPI