AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene072291


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene072291.t1 MTR_8g012960 97.235 217 6 0 1 217 37 253 3.33e-153 426
MS.gene072291.t1 MTR_7g056147 66.063 221 54 7 1 217 1 204 3.83e-84 249
MS.gene072291.t1 MTR_7g056147 66.063 221 54 7 1 217 38 241 1.08e-83 249
MS.gene072291.t1 MTR_7g056147 66.063 221 54 7 1 217 39 242 1.65e-83 248
MS.gene072291.t1 MTR_5g096030 48.485 66 31 2 155 217 2 67 9.26e-13 62.0
MS.gene072291.t1 MTR_4g104085 50.000 54 27 0 155 208 19 72 2.29e-11 58.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene072291.t1 AT3G25070 35.294 221 87 8 1 217 37 205 1.10e-21 89.4
MS.gene072291.t1 AT3G25070 37.755 196 66 8 1 192 50 193 1.35e-21 89.4
MS.gene072291.t1 AT5G55850 55.319 47 19 1 155 201 3 47 2.70e-11 58.2
MS.gene072291.t1 AT5G55850 55.319 47 19 1 155 201 3 47 2.87e-11 58.9
MS.gene072291.t1 AT5G55850 55.319 47 19 1 155 201 19 63 7.79e-11 58.2

Find 76 sgRNAs with CRISPR-Local

Find 86 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGAACAGCTGCACCTTTATC+AGG 0.262008 8.3:-75393456 None:intergenic
CTCTGAATTGGCTCTGGGTT+TGG 0.278752 8.3:-75392935 None:intergenic
CCTGAGCTTTGTCATTAGTT+TGG 0.314715 8.3:-75393620 None:intergenic
CCAGCAGTACTTTGTCTTGA+AGG 0.363594 8.3:-75393129 None:intergenic
GGTGCAGCTGTTCCAAAGTT+TGG 0.378951 8.3:+75393465 MS.gene072291:CDS
GAGGAGAGACAGGGTGGGGC+CGG 0.380564 8.3:+75393552 MS.gene072291:CDS
AGATTTGCAATATGTTAAAC+AGG 0.383444 8.3:-75393651 None:intergenic
ATAACATGCGGCCTCTCATT+AGG 0.384811 8.3:-75393591 None:intergenic
GCCAATTCAGAGGATCTATC+TGG 0.386827 8.3:+75392946 MS.gene072291:CDS
ATTGGCTCTGGGTTTGGTAT+TGG 0.387368 8.3:-75392929 None:intergenic
CGGCCTCTCATTAGGTGTGC+CGG 0.392536 8.3:-75393583 None:intergenic
TCCAGATAGATCCTCTGAAT+TGG 0.407045 8.3:-75392947 None:intergenic
TGTACCATGGCTGCTGTCAT+AGG 0.408751 8.3:-75393244 None:intergenic
AGGTGTGCCGGGTGCATGTC+CGG 0.413031 8.3:-75393571 None:intergenic
CAAAGTAAGAGAGGAGAGAC+AGG 0.416279 8.3:+75393542 MS.gene072291:CDS
TGTTCCAAAGTTTGGTGATT+GGG 0.423365 8.3:+75393473 MS.gene072291:CDS
TAGATCCTCTGAATTGGCTC+TGG 0.423435 8.3:-75392941 None:intergenic
CTGTTCCAAAGTTTGGTGAT+TGG 0.430040 8.3:+75393472 MS.gene072291:CDS
CCTTCAAGACAAAGTACTGC+TGG 0.444639 8.3:+75393129 MS.gene072291:CDS
CTGATGTTGCAGCCTGATAA+AGG 0.447195 8.3:+75393444 MS.gene072291:intron
GTTTCATCCCCTCGATTAGC+TGG 0.459418 8.3:-75393288 None:intergenic
GGTCTTAACCGAGATCTTCC+CGG 0.461138 8.3:-75393267 None:intergenic
CTTTGTCATTAGTTTGGTTG+CGG 0.463206 8.3:-75393614 None:intergenic
AAGACGAGGTGTAGAATCAT+TGG 0.469444 8.3:-75393076 None:intergenic
CTGGAAGAGATAGTCCCTCT+TGG 0.470130 8.3:+75393205 MS.gene072291:CDS
GGCCTCTCATTAGGTGTGCC+GGG 0.483664 8.3:-75393582 None:intergenic
ACGGTCACCCCTTCATCGTC+AGG 0.485020 8.3:+75393170 MS.gene072291:CDS
ACATCCCAATCACCAAACTT+TGG 0.494032 8.3:-75393477 None:intergenic
GGTTTGGTATTGGAAGGATG+TGG 0.498106 8.3:-75392919 None:intergenic
GAGCTCTAGCCTGACGATGA+AGG 0.501709 8.3:-75393179 None:intergenic
GTATAGCCATCAGCTGATGC+AGG 0.504777 8.3:-75393507 None:intergenic
TGGAAGAGATAGTCCCTCTT+GGG 0.516000 8.3:+75393206 MS.gene072291:CDS
TAAGCAATATGTTGACTCTC+CGG 0.517127 8.3:+75393023 MS.gene072291:CDS
GGATCTATCTGGAAAGGGAT+TGG 0.518744 8.3:+75392957 MS.gene072291:CDS
GCCATGACAATGTAAGCAAT+AGG 0.523259 8.3:+75393049 MS.gene072291:CDS
GGAGAGTCAACATATTGCTT+AGG 0.526243 8.3:-75393021 None:intergenic
AGCTCTAGCCTGACGATGAA+GGG 0.528571 8.3:-75393178 None:intergenic
TCCTATTGCTTACATTGTCA+TGG 0.532366 8.3:-75393050 None:intergenic
AATGATTCTACACCTCGTCT+TGG 0.537591 8.3:+75393078 MS.gene072291:CDS
TCAGAGGATCTATCTGGAAA+GGG 0.538299 8.3:+75392952 MS.gene072291:CDS
CTTACATTGTCATGGCGAGC+CGG 0.540021 8.3:-75393042 None:intergenic
AGATCCTCTGAATTGGCTCT+GGG 0.540595 8.3:-75392940 None:intergenic
GCTCTGGGTTTGGTATTGGA+AGG 0.547279 8.3:-75392925 None:intergenic
TTCAGAGGATCTATCTGGAA+AGG 0.551409 8.3:+75392951 MS.gene072291:CDS
ATGGTACACCGGGAAGATCT+CGG 0.552829 8.3:+75393259 MS.gene072291:CDS
GGTGGGGCCGGACATGCACC+CGG 0.553291 8.3:+75393564 MS.gene072291:CDS
TCTACACCTCGTCTTGGAGT+AGG 0.553839 8.3:+75393084 MS.gene072291:CDS
CGTCAGGCTAGAGCTCCTGC+TGG 0.560727 8.3:+75393186 MS.gene072291:CDS
CCAAACTAATGACAAAGCTC+AGG 0.564139 8.3:+75393620 MS.gene072291:CDS
GACAGCAGCCATGGTACACC+GGG 0.575291 8.3:+75393249 MS.gene072291:CDS
GAGGGACTATCTCTTCCAGC+AGG 0.582014 8.3:-75393201 None:intergenic
AATTCCTATGACAGCAGCCA+TGG 0.582281 8.3:+75393240 MS.gene072291:CDS
TTATTCTTTCCTTCCCAAGA+GGG 0.587434 8.3:-75393219 None:intergenic
GGTCTGAGCACAGCGTTGAA+CGG 0.589876 8.3:+75393151 MS.gene072291:CDS
CTTCAAGACAAAGTACTGCT+GGG 0.593023 8.3:+75393130 MS.gene072291:CDS
TGACAGCAGCCATGGTACAC+CGG 0.594877 8.3:+75393248 MS.gene072291:CDS
GGTTAAGACCAGCTAATCGA+GGG 0.601360 8.3:+75393280 MS.gene072291:CDS
TTGCAATATGTTAAACAGGA+GGG 0.612341 8.3:-75393647 None:intergenic
AGTAACCCTGCATCAGCTGA+TGG 0.613824 8.3:+75393501 MS.gene072291:CDS
GTTTGGTTGCGGATAACATG+CGG 0.615150 8.3:-75393603 None:intergenic
AAGAGAGGAGAGACAGGGTG+GGG 0.615205 8.3:+75393548 MS.gene072291:CDS
GAGATCTTCCCGGTGTACCA+TGG 0.615868 8.3:-75393257 None:intergenic
CGGTTAAGACCAGCTAATCG+AGG 0.616969 8.3:+75393279 MS.gene072291:CDS
CACCCGGCACACCTAATGAG+AGG 0.620241 8.3:+75393580 MS.gene072291:CDS
GCTCTAGCCTGACGATGAAG+GGG 0.622879 8.3:-75393177 None:intergenic
TTTGCAATATGTTAAACAGG+AGG 0.630782 8.3:-75393648 None:intergenic
TAAGAGAGGAGAGACAGGGT+GGG 0.631626 8.3:+75393547 MS.gene072291:CDS
TATAGCCATCAGCTGATGCA+GGG 0.633969 8.3:-75393506 None:intergenic
GAGTCGCATAAAAGTCTCGA+TGG 0.635478 8.3:+75392994 MS.gene072291:CDS
AGAGATAGTCCCTCTTGGGA+AGG 0.636769 8.3:+75393210 MS.gene072291:CDS
ATTATTCTTTCCTTCCCAAG+AGG 0.649386 8.3:-75393220 None:intergenic
CAAACCCAGAGCCAATTCAG+AGG 0.659677 8.3:+75392936 MS.gene072291:CDS
GTAAGAGAGGAGAGACAGGG+TGG 0.661332 8.3:+75393546 MS.gene072291:CDS
AAAGTAAGAGAGGAGAGACA+GGG 0.706360 8.3:+75393543 MS.gene072291:CDS
GTTAAGACCAGCTAATCGAG+GGG 0.714196 8.3:+75393281 MS.gene072291:CDS
GCAGAACCTACTCCAAGACG+AGG 0.735764 8.3:-75393090 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! GCATATATATTATTGTACCT+TGG + chr8.3:75393374-75393393 MS.gene072291:intron 25.0%
!! TTGCGAATATGTTATGATTT+TGG + chr8.3:75393417-75393436 MS.gene072291:intron 25.0%
AAAATATCACGATGATTCCA+AGG - chr8.3:75393394-75393413 None:intergenic 30.0%
! ACTAAATCGACATTTTCTTC+AGG - chr8.3:75392874-75392893 None:intergenic 30.0%
! CATTTTCAACAAAGTAAGAG+AGG + chr8.3:75393533-75393552 MS.gene072291:CDS 30.0%
! CTAAATCGACATTTTCTTCA+GGG - chr8.3:75392873-75392892 None:intergenic 30.0%
ATTATTCTTTCCTTCCCAAG+AGG - chr8.3:75393223-75393242 None:intergenic 35.0%
TCCTATTGCTTACATTGTCA+TGG - chr8.3:75393053-75393072 None:intergenic 35.0%
TTATTCTTTCCTTCCCAAGA+GGG - chr8.3:75393222-75393241 None:intergenic 35.0%
! ACATTTTCTTCAGGGTCATT+CGG - chr8.3:75392865-75392884 None:intergenic 35.0%
! CTTTGTCATTAGTTTGGTTG+CGG - chr8.3:75393617-75393636 None:intergenic 35.0%
! TGTTCCAAAGTTTGGTGATT+GGG + chr8.3:75393473-75393492 MS.gene072291:CDS 35.0%
! TTTGTCTTGAAGGTTTTCGT+CGG - chr8.3:75393122-75393141 None:intergenic 35.0%
!! TAAGCAATATGTTGACTCTC+CGG + chr8.3:75393023-75393042 MS.gene072291:CDS 35.0%
AAAGTAAGAGAGGAGAGACA+GGG + chr8.3:75393543-75393562 MS.gene072291:CDS 40.0%
AAGACGAGGTGTAGAATCAT+TGG - chr8.3:75393079-75393098 None:intergenic 40.0%
AATGATTCTACACCTCGTCT+TGG + chr8.3:75393078-75393097 MS.gene072291:CDS 40.0%
ACATCCCAATCACCAAACTT+TGG - chr8.3:75393480-75393499 None:intergenic 40.0%
CCAAACTAATGACAAAGCTC+AGG + chr8.3:75393620-75393639 MS.gene072291:CDS 40.0%
GCCATGACAATGTAAGCAAT+AGG + chr8.3:75393049-75393068 MS.gene072291:CDS 40.0%
GGAGAGTCAACATATTGCTT+AGG - chr8.3:75393024-75393043 None:intergenic 40.0%
TCAGAGGATCTATCTGGAAA+GGG + chr8.3:75392952-75392971 MS.gene072291:CDS 40.0%
TCCAGATAGATCCTCTGAAT+TGG - chr8.3:75392950-75392969 None:intergenic 40.0%
TCCTATGTTATCACCATGCA+TGG + chr8.3:75393333-75393352 MS.gene072291:intron 40.0%
TGCATGGTGATAACATAGGA+TGG - chr8.3:75393333-75393352 None:intergenic 40.0%
TTCAGAGGATCTATCTGGAA+AGG + chr8.3:75392951-75392970 MS.gene072291:CDS 40.0%
TTTACTCTTCATGCCATGCA+TGG - chr8.3:75393349-75393368 None:intergenic 40.0%
! CATTTTCTTCAGGGTCATTC+GGG - chr8.3:75392864-75392883 None:intergenic 40.0%
! CCTGAGCTTTGTCATTAGTT+TGG - chr8.3:75393623-75393642 None:intergenic 40.0%
! CTGTTCCAAAGTTTGGTGAT+TGG + chr8.3:75393472-75393491 MS.gene072291:CDS 40.0%
!! CTTCAAGACAAAGTACTGCT+GGG + chr8.3:75393130-75393149 MS.gene072291:CDS 40.0%
AATTCCTATGACAGCAGCCA+TGG + chr8.3:75393240-75393259 MS.gene072291:CDS 45.0%
ATAACATGCGGCCTCTCATT+AGG - chr8.3:75393594-75393613 None:intergenic 45.0%
CAAAGTAAGAGAGGAGAGAC+AGG + chr8.3:75393542-75393561 MS.gene072291:CDS 45.0%
CCAGCAGTACTTTGTCTTGA+AGG - chr8.3:75393132-75393151 None:intergenic 45.0%
CTGATGTTGCAGCCTGATAA+AGG + chr8.3:75393444-75393463 MS.gene072291:intron 45.0%
GAGTCGCATAAAAGTCTCGA+TGG + chr8.3:75392994-75393013 MS.gene072291:CDS 45.0%
GCCAATTCAGAGGATCTATC+TGG + chr8.3:75392946-75392965 MS.gene072291:CDS 45.0%
GCCATGCATGGTGATAACAT+AGG - chr8.3:75393337-75393356 None:intergenic 45.0%
GGATCTATCTGGAAAGGGAT+TGG + chr8.3:75392957-75392976 MS.gene072291:CDS 45.0%
GGTTAAGACCAGCTAATCGA+GGG + chr8.3:75393280-75393299 MS.gene072291:CDS 45.0%
GTTAAGACCAGCTAATCGAG+GGG + chr8.3:75393281-75393300 MS.gene072291:CDS 45.0%
GTTTGGTTGCGGATAACATG+CGG - chr8.3:75393606-75393625 None:intergenic 45.0%
TATAGCCATCAGCTGATGCA+GGG - chr8.3:75393509-75393528 None:intergenic 45.0%
TGGAAGAGATAGTCCCTCTT+GGG + chr8.3:75393206-75393225 MS.gene072291:CDS 45.0%
! AGATCCTCTGAATTGGCTCT+GGG - chr8.3:75392943-75392962 None:intergenic 45.0%
! GGTTTGGTATTGGAAGGATG+TGG - chr8.3:75392922-75392941 None:intergenic 45.0%
! TAGATCCTCTGAATTGGCTC+TGG - chr8.3:75392944-75392963 None:intergenic 45.0%
!! ATTGGCTCTGGGTTTGGTAT+TGG - chr8.3:75392932-75392951 None:intergenic 45.0%
!! CCTTCAAGACAAAGTACTGC+TGG + chr8.3:75393129-75393148 MS.gene072291:CDS 45.0%
AGAGATAGTCCCTCTTGGGA+AGG + chr8.3:75393210-75393229 MS.gene072291:CDS 50.0%
AGTAACCCTGCATCAGCTGA+TGG + chr8.3:75393501-75393520 MS.gene072291:CDS 50.0%
ATGGTACACCGGGAAGATCT+CGG + chr8.3:75393259-75393278 MS.gene072291:CDS 50.0%
CAAACCCAGAGCCAATTCAG+AGG + chr8.3:75392936-75392955 MS.gene072291:CDS 50.0%
CGGTTAAGACCAGCTAATCG+AGG + chr8.3:75393279-75393298 MS.gene072291:CDS 50.0%
CTGGAAGAGATAGTCCCTCT+TGG + chr8.3:75393205-75393224 MS.gene072291:CDS 50.0%
CTTACATTGTCATGGCGAGC+CGG - chr8.3:75393045-75393064 None:intergenic 50.0%
GGAACAGCTGCACCTTTATC+AGG - chr8.3:75393459-75393478 None:intergenic 50.0%
GGTCTTAACCGAGATCTTCC+CGG - chr8.3:75393270-75393289 None:intergenic 50.0%
GGTGCAGCTGTTCCAAAGTT+TGG + chr8.3:75393465-75393484 MS.gene072291:CDS 50.0%
GTATAGCCATCAGCTGATGC+AGG - chr8.3:75393510-75393529 None:intergenic 50.0%
GTTTCATCCCCTCGATTAGC+TGG - chr8.3:75393291-75393310 None:intergenic 50.0%
TAAGAGAGGAGAGACAGGGT+GGG + chr8.3:75393547-75393566 MS.gene072291:CDS 50.0%
TCTACACCTCGTCTTGGAGT+AGG + chr8.3:75393084-75393103 MS.gene072291:CDS 50.0%
TGTACCATGGCTGCTGTCAT+AGG - chr8.3:75393247-75393266 None:intergenic 50.0%
! AGCTCTAGCCTGACGATGAA+GGG - chr8.3:75393181-75393200 None:intergenic 50.0%
! GCTCTGGGTTTGGTATTGGA+AGG - chr8.3:75392928-75392947 None:intergenic 50.0%
!! CTCTGAATTGGCTCTGGGTT+TGG - chr8.3:75392938-75392957 None:intergenic 50.0%
AAGAGAGGAGAGACAGGGTG+GGG + chr8.3:75393548-75393567 MS.gene072291:CDS 55.0%
GAGATCTTCCCGGTGTACCA+TGG - chr8.3:75393260-75393279 None:intergenic 55.0%
GAGGGACTATCTCTTCCAGC+AGG - chr8.3:75393204-75393223 None:intergenic 55.0%
GCAGAACCTACTCCAAGACG+AGG - chr8.3:75393093-75393112 None:intergenic 55.0%
GCTCTAGCCTGACGATGAAG+GGG - chr8.3:75393180-75393199 None:intergenic 55.0%
GTAAGAGAGGAGAGACAGGG+TGG + chr8.3:75393546-75393565 MS.gene072291:CDS 55.0%
TGACAGCAGCCATGGTACAC+CGG + chr8.3:75393248-75393267 MS.gene072291:CDS 55.0%
! GAGCTCTAGCCTGACGATGA+AGG - chr8.3:75393182-75393201 None:intergenic 55.0%
! GGTCTGAGCACAGCGTTGAA+CGG + chr8.3:75393151-75393170 MS.gene072291:CDS 55.0%
ACGGTCACCCCTTCATCGTC+AGG + chr8.3:75393170-75393189 MS.gene072291:CDS 60.0%
CACCCGGCACACCTAATGAG+AGG + chr8.3:75393580-75393599 MS.gene072291:CDS 60.0%
CGGCCTCTCATTAGGTGTGC+CGG - chr8.3:75393586-75393605 None:intergenic 60.0%
GACAGCAGCCATGGTACACC+GGG + chr8.3:75393249-75393268 MS.gene072291:CDS 60.0%
GGCCTCTCATTAGGTGTGCC+GGG - chr8.3:75393585-75393604 None:intergenic 60.0%
AGGTGTGCCGGGTGCATGTC+CGG - chr8.3:75393574-75393593 None:intergenic 65.0%
CGTCAGGCTAGAGCTCCTGC+TGG + chr8.3:75393186-75393205 MS.gene072291:CDS 65.0%
GAGGAGAGACAGGGTGGGGC+CGG + chr8.3:75393552-75393571 MS.gene072291:CDS 70.0%
GGTGGGGCCGGACATGCACC+CGG + chr8.3:75393564-75393583 MS.gene072291:CDS 75.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 75392853 75393659 75392853 ID=MS.gene072291
chr8.3 mRNA 75392853 75393659 75392853 ID=MS.gene072291.t1;Parent=MS.gene072291
chr8.3 exon 75392853 75393311 75392853 ID=MS.gene072291.t1.exon1;Parent=MS.gene072291.t1
chr8.3 CDS 75392853 75393311 75392853 ID=cds.MS.gene072291.t1;Parent=MS.gene072291.t1
chr8.3 exon 75393456 75393659 75393456 ID=MS.gene072291.t1.exon2;Parent=MS.gene072291.t1
chr8.3 CDS 75393456 75393659 75393456 ID=cds.MS.gene072291.t1;Parent=MS.gene072291.t1
Gene Sequence

>MS.gene072291

ATGATAAACCCGAATGACCCTGAAGAAAATGTCGATTTAGTTCTTGATAATTCATCGTCTGATCATCCACATCCTTCCAATACCAAACCCAGAGCCAATTCAGAGGATCTATCTGGAAAGGGATTGGTGCAATCAACAATTGAGTCGCATAAAAGTCTCGATGGTGATCCTAAGCAATATGTTGACTCTCCGGCTCGCCATGACAATGTAAGCAATAGGAGTTCCAATGATTCTACACCTCGTCTTGGAGTAGGTTCTGCTGATAACCGACGAAAACCTTCAAGACAAAGTACTGCTGGGTCTGAGCACAGCGTTGAACGGTCACCCCTTCATCGTCAGGCTAGAGCTCCTGCTGGAAGAGATAGTCCCTCTTGGGAAGGAAAGAATAATTCCTATGACAGCAGCCATGGTACACCGGGAAGATCTCGGTTAAGACCAGCTAATCGAGGGGATGAAACTGTTAGTTTTTCTTTTCTTCCATCCTATGTTATCACCATGCATGGCATGAAGAGTAAAAATCTGCATATATATTATTGTACCTTGGAATCATCGTGATATTTTCTATTGCGAATATGTTATGATTTTGGCATTCTGATGTTGCAGCCTGATAAAGGTGCAGCTGTTCCAAAGTTTGGTGATTGGGATGTGAGTAACCCTGCATCAGCTGATGGCTATACTCACATTTTCAACAAAGTAAGAGAGGAGAGACAGGGTGGGGCCGGACATGCACCCGGCACACCTAATGAGAGGCCGCATGTTATCCGCAACCAAACTAATGACAAAGCTCAGGTATGCCCTCCTGTTTAA

Protein sequence

>MS.gene072291.t1

MINPNDPEENVDLVLDNSSSDHPHPSNTKPRANSEDLSGKGLVQSTIESHKSLDGDPKQYVDSPARHDNVSNRSSNDSTPRLGVGSADNRRKPSRQSTAGSEHSVERSPLHRQARAPAGRDSPSWEGKNNSYDSSHGTPGRSRLRPANRGDETPDKGAAVPKFGDWDVSNPASADGYTHIFNKVREERQGGAGHAPGTPNERPHVIRNQTNDKAQVCPPV