Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059943.t1 | XP_013457732.1 | 98.5 | 206 | 3 | 0 | 1 | 206 | 1 | 206 | 3.10E-109 | 404.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059943.t1 | Q9FGS5 | 51.7 | 209 | 90 | 5 | 1 | 202 | 1 | 205 | 5.8e-48 | 192.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059943.t1 | I3SMV0 | 98.5 | 206 | 3 | 0 | 1 | 206 | 1 | 206 | 2.2e-109 | 404.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049147 | MS.gene059943 | 0.800936 | 1.14E-48 | -1.69E-46 |
| MS.gene049148 | MS.gene059943 | 0.800751 | 1.24E-48 | -1.69E-46 |
| MS.gene049634 | MS.gene059943 | 0.80546 | 1.32E-49 | -1.69E-46 |
| MS.gene050163 | MS.gene059943 | -0.800066 | 1.72E-48 | -1.69E-46 |
| MS.gene050173 | MS.gene059943 | 0.831747 | 1.40E-55 | -1.69E-46 |
| MS.gene050216 | MS.gene059943 | -0.829941 | 3.88E-55 | -1.69E-46 |
| MS.gene050381 | MS.gene059943 | 0.81652 | 5.30E-52 | -1.69E-46 |
| MS.gene050504 | MS.gene059943 | -0.816295 | 5.95E-52 | -1.69E-46 |
| MS.gene053561 | MS.gene059943 | 0.819531 | 1.11E-52 | -1.69E-46 |
| MS.gene054616 | MS.gene059943 | 0.819972 | 8.77E-53 | -1.69E-46 |
| MS.gene058171 | MS.gene059943 | 0.823332 | 1.47E-53 | -1.69E-46 |
| MS.gene058173 | MS.gene059943 | 0.811102 | 8.28E-51 | -1.69E-46 |
| MS.gene058175 | MS.gene059943 | 0.823833 | 1.12E-53 | -1.69E-46 |
| MS.gene058840 | MS.gene059943 | 0.840535 | 8.20E-58 | -1.69E-46 |
| MS.gene059943 | MS.gene059944 | 0.820108 | 8.16E-53 | -1.69E-46 |
| MS.gene059943 | MS.gene06136 | 0.855677 | 5.37E-62 | -1.69E-46 |
| MS.gene059943 | MS.gene062071 | 0.803964 | 2.71E-49 | -1.69E-46 |
| MS.gene059943 | MS.gene062923 | -0.813382 | 2.63E-51 | -1.69E-46 |
| MS.gene059943 | MS.gene06346 | -0.801725 | 7.86E-49 | -1.69E-46 |
| MS.gene059943 | MS.gene063635 | 0.813867 | 2.06E-51 | -1.69E-46 |
| MS.gene059943 | MS.gene064019 | 0.843203 | 1.62E-58 | -1.69E-46 |
| MS.gene059943 | MS.gene067047 | -0.810076 | 1.38E-50 | -1.69E-46 |
| MS.gene059943 | MS.gene067978 | -0.804221 | 2.40E-49 | -1.69E-46 |
| MS.gene059943 | MS.gene068043 | 0.81767 | 2.92E-52 | -1.69E-46 |
| MS.gene059943 | MS.gene068320 | 0.810349 | 1.20E-50 | -1.69E-46 |
| MS.gene059943 | MS.gene071843 | 0.824006 | 1.02E-53 | -1.69E-46 |
| MS.gene059943 | MS.gene071942 | 0.845448 | 4.04E-59 | -1.69E-46 |
| MS.gene059943 | MS.gene072291 | 0.818184 | 2.24E-52 | -1.69E-46 |
| MS.gene059943 | MS.gene072806 | 0.813812 | 2.12E-51 | -1.69E-46 |
| MS.gene059943 | MS.gene07427 | -0.81868 | 1.73E-52 | -1.69E-46 |
| MS.gene059943 | MS.gene07428 | -0.818082 | 2.36E-52 | -1.69E-46 |
| MS.gene059943 | MS.gene07448 | 0.816991 | 4.16E-52 | -1.69E-46 |
| MS.gene059943 | MS.gene074503 | -0.80156 | 8.50E-49 | -1.69E-46 |
| MS.gene059943 | MS.gene08520 | -0.80518 | 1.51E-49 | -1.69E-46 |
| MS.gene059943 | MS.gene09722 | -0.803748 | 3.01E-49 | -1.69E-46 |
| MS.gene059943 | MS.gene20093 | 0.81175 | 5.99E-51 | -1.69E-46 |
| MS.gene059943 | MS.gene20808 | 0.801522 | 8.65E-49 | -1.69E-46 |
| MS.gene059943 | MS.gene21370 | 0.822136 | 2.78E-53 | -1.69E-46 |
| MS.gene059943 | MS.gene21595 | 0.83651 | 8.97E-57 | -1.69E-46 |
| MS.gene059943 | MS.gene21600 | -0.809194 | 2.13E-50 | -1.69E-46 |
| MS.gene059943 | MS.gene21867 | 0.802155 | 6.42E-49 | -1.69E-46 |
| MS.gene059943 | MS.gene22597 | 0.831719 | 1.42E-55 | -1.69E-46 |
| MS.gene059943 | MS.gene23018 | -0.807711 | 4.42E-50 | -1.69E-46 |
| MS.gene059943 | MS.gene24359 | 0.802161 | 6.40E-49 | -1.69E-46 |
| MS.gene059943 | MS.gene25483 | -0.801069 | 1.07E-48 | -1.69E-46 |
| MS.gene059943 | MS.gene25666 | -0.806478 | 8.07E-50 | -1.69E-46 |
| MS.gene059943 | MS.gene25675 | -0.802503 | 5.44E-49 | -1.69E-46 |
| MS.gene059943 | MS.gene26152 | 0.809122 | 2.21E-50 | -1.69E-46 |
| MS.gene059943 | MS.gene26384 | -0.807023 | 6.19E-50 | -1.69E-46 |
| MS.gene059943 | MS.gene26662 | -0.805968 | 1.03E-49 | -1.69E-46 |
| MS.gene059943 | MS.gene27061 | -0.802964 | 4.37E-49 | -1.69E-46 |
| MS.gene059943 | MS.gene27550 | 0.819767 | 9.77E-53 | -1.69E-46 |
| MS.gene059943 | MS.gene27711 | 0.810089 | 1.37E-50 | -1.69E-46 |
| MS.gene059943 | MS.gene27779 | 0.828695 | 7.79E-55 | -1.69E-46 |
| MS.gene059943 | MS.gene27827 | 0.841198 | 5.50E-58 | -1.69E-46 |
| MS.gene059943 | MS.gene28444 | -0.814487 | 1.50E-51 | -1.69E-46 |
| MS.gene059943 | MS.gene29026 | 0.800226 | 1.59E-48 | -1.69E-46 |
| MS.gene059943 | MS.gene29460 | -0.848945 | 4.43E-60 | -1.69E-46 |
| MS.gene059943 | MS.gene31404 | 0.823136 | 1.63E-53 | -1.69E-46 |
| MS.gene059943 | MS.gene32026 | -0.809588 | 1.76E-50 | -1.69E-46 |
| MS.gene059943 | MS.gene34025 | -0.825282 | 5.10E-54 | -1.69E-46 |
| MS.gene059943 | MS.gene35546 | 0.800619 | 1.32E-48 | -1.69E-46 |
| MS.gene059943 | MS.gene35633 | 0.822466 | 2.33E-53 | -1.69E-46 |
| MS.gene059943 | MS.gene36927 | 0.827536 | 1.48E-54 | -1.69E-46 |
| MS.gene059943 | MS.gene37192 | 0.802092 | 6.61E-49 | -1.69E-46 |
| MS.gene059943 | MS.gene37538 | 0.806598 | 7.61E-50 | -1.69E-46 |
| MS.gene059943 | MS.gene37609 | 0.810678 | 1.02E-50 | -1.69E-46 |
| MS.gene059943 | MS.gene38094 | 0.83918 | 1.85E-57 | -1.69E-46 |
| MS.gene059943 | MS.gene38095 | 0.855559 | 5.82E-62 | -1.69E-46 |
| MS.gene059943 | MS.gene38097 | 0.862688 | 4.25E-64 | -1.69E-46 |
| MS.gene059943 | MS.gene38155 | 0.825619 | 4.25E-54 | -1.69E-46 |
| MS.gene059943 | MS.gene38172 | 0.833623 | 4.80E-56 | -1.69E-46 |
| MS.gene059943 | MS.gene38173 | 0.810362 | 1.20E-50 | -1.69E-46 |
| MS.gene059943 | MS.gene38741 | 0.816478 | 5.42E-52 | -1.69E-46 |
| MS.gene059943 | MS.gene39520 | -0.808234 | 3.42E-50 | -1.69E-46 |
| MS.gene059943 | MS.gene39533 | 0.802176 | 6.35E-49 | -1.69E-46 |
| MS.gene059943 | MS.gene39782 | 0.813214 | 2.87E-51 | -1.69E-46 |
| MS.gene059943 | MS.gene40699 | 0.81338 | 2.64E-51 | -1.69E-46 |
| MS.gene059943 | MS.gene40943 | -0.80144 | 8.99E-49 | -1.69E-46 |
| MS.gene059943 | MS.gene41340 | 0.809648 | 1.71E-50 | -1.69E-46 |
| MS.gene059943 | MS.gene43433 | 0.811443 | 6.98E-51 | -1.69E-46 |
| MS.gene059943 | MS.gene44781 | -0.809705 | 1.66E-50 | -1.69E-46 |
| MS.gene059943 | MS.gene46024 | 0.80846 | 3.06E-50 | -1.69E-46 |
| MS.gene059943 | MS.gene46027 | 0.825797 | 3.85E-54 | -1.69E-46 |
| MS.gene059943 | MS.gene46433 | 0.822445 | 2.36E-53 | -1.69E-46 |
| MS.gene059943 | MS.gene47031 | 0.819232 | 1.29E-52 | -1.69E-46 |
| MS.gene059943 | MS.gene47889 | -0.804887 | 1.74E-49 | -1.69E-46 |
| MS.gene059943 | MS.gene48315 | 0.911083 | 9.08E-83 | -1.69E-46 |
| MS.gene059943 | MS.gene49181 | 0.816783 | 4.63E-52 | -1.69E-46 |
| MS.gene059943 | MS.gene49499 | -0.805843 | 1.10E-49 | -1.69E-46 |
| MS.gene059943 | MS.gene50712 | -0.815488 | 9.01E-52 | -1.69E-46 |
| MS.gene059943 | MS.gene52062 | 0.80764 | 4.58E-50 | -1.69E-46 |
| MS.gene059943 | MS.gene54277 | 0.831889 | 1.29E-55 | -1.69E-46 |
| MS.gene059943 | MS.gene54782 | -0.820394 | 7.02E-53 | -1.69E-46 |
| MS.gene059943 | MS.gene54882 | 0.801479 | 8.83E-49 | -1.69E-46 |
| MS.gene059943 | MS.gene55036 | 0.835164 | 1.97E-56 | -1.69E-46 |
| MS.gene059943 | MS.gene56456 | -0.812885 | 3.38E-51 | -1.69E-46 |
| MS.gene059943 | MS.gene57949 | 0.834153 | 3.53E-56 | -1.69E-46 |
| MS.gene059943 | MS.gene58464 | 0.843797 | 1.12E-58 | -1.69E-46 |
| MS.gene059943 | MS.gene59395 | 0.803661 | 3.13E-49 | -1.69E-46 |
| MS.gene059943 | MS.gene59555 | 0.847307 | 1.26E-59 | -1.69E-46 |
| MS.gene059943 | MS.gene60649 | 0.823126 | 1.64E-53 | -1.69E-46 |
| MS.gene059943 | MS.gene63020 | -0.80057 | 1.35E-48 | -1.69E-46 |
| MS.gene059943 | MS.gene64686 | 0.800196 | 1.61E-48 | -1.69E-46 |
| MS.gene059943 | MS.gene65990 | -0.809041 | 2.30E-50 | -1.69E-46 |
| MS.gene059943 | MS.gene66360 | 0.812588 | 3.93E-51 | -1.69E-46 |
| MS.gene059943 | MS.gene66463 | 0.801486 | 8.80E-49 | -1.69E-46 |
| MS.gene059943 | MS.gene67232 | -0.807313 | 5.37E-50 | -1.69E-46 |
| MS.gene059943 | MS.gene67682 | 0.856677 | 2.74E-62 | -1.69E-46 |
| MS.gene059943 | MS.gene68181 | 0.814568 | 1.44E-51 | -1.69E-46 |
| MS.gene059943 | MS.gene68457 | -0.817189 | 3.75E-52 | -1.69E-46 |
| MS.gene059943 | MS.gene68458 | -0.832144 | 1.12E-55 | -1.69E-46 |
| MS.gene059943 | MS.gene68851 | 0.836161 | 1.10E-56 | -1.69E-46 |
| MS.gene059943 | MS.gene68948 | 0.84046 | 8.58E-58 | -1.69E-46 |
| MS.gene059943 | MS.gene70692 | -0.809377 | 1.95E-50 | -1.69E-46 |
| MS.gene059943 | MS.gene71268 | -0.803926 | 2.76E-49 | -1.69E-46 |
| MS.gene059943 | MS.gene73256 | 0.824199 | 9.19E-54 | -1.69E-46 |
| MS.gene059943 | MS.gene73753 | 0.803743 | 3.01E-49 | -1.69E-46 |
| MS.gene059943 | MS.gene74217 | -0.801565 | 8.48E-49 | -1.69E-46 |
| MS.gene059943 | MS.gene75048 | 0.835372 | 1.74E-56 | -1.69E-46 |
| MS.gene059943 | MS.gene75561 | -0.802614 | 5.16E-49 | -1.69E-46 |
| MS.gene059943 | MS.gene76573 | 0.811976 | 5.35E-51 | -1.69E-46 |
| MS.gene059943 | MS.gene76797 | -0.803325 | 3.68E-49 | -1.69E-46 |
| MS.gene059943 | MS.gene77223 | 0.813606 | 2.35E-51 | -1.69E-46 |
| MS.gene059943 | MS.gene77471 | 0.812063 | 5.12E-51 | -1.69E-46 |
| MS.gene059943 | MS.gene84947 | 0.803671 | 3.12E-49 | -1.69E-46 |
| MS.gene059943 | MS.gene85293 | 0.800697 | 1.28E-48 | -1.69E-46 |
| MS.gene059943 | MS.gene85601 | -0.81921 | 1.31E-52 | -1.69E-46 |
| MS.gene059943 | MS.gene85638 | 0.803231 | 3.85E-49 | -1.69E-46 |
| MS.gene059943 | MS.gene85996 | 0.84815 | 7.36E-60 | -1.69E-46 |
| MS.gene059943 | MS.gene86222 | 0.846778 | 1.75E-59 | -1.69E-46 |
| MS.gene059943 | MS.gene88816 | 0.815925 | 7.20E-52 | -1.69E-46 |
| MS.gene059943 | MS.gene89218 | -0.818344 | 2.06E-52 | -1.69E-46 |
| MS.gene059943 | MS.gene90201 | 0.81977 | 9.76E-53 | -1.69E-46 |
| MS.gene059943 | MS.gene90796 | -0.802787 | 4.75E-49 | -1.69E-46 |
| MS.gene059943 | MS.gene91973 | 0.815894 | 7.32E-52 | -1.69E-46 |
| MS.gene059943 | MS.gene91977 | 0.814486 | 1.50E-51 | -1.69E-46 |
| MS.gene059943 | MS.gene92589 | 0.814137 | 1.80E-51 | -1.69E-46 |
| MS.gene059943 | MS.gene92808 | 0.809715 | 1.65E-50 | -1.69E-46 |
| MS.gene059943 | MS.gene94790 | 0.821898 | 3.16E-53 | -1.69E-46 |
| MS.gene059943 | MS.gene95272 | 0.841802 | 3.81E-58 | -1.69E-46 |
| MS.gene059943 | MS.gene96524 | -0.800144 | 1.65E-48 | -1.69E-46 |
| MS.gene059943 | MS.gene97158 | 0.803258 | 3.80E-49 | -1.69E-46 |
| MS.gene059943 | MS.gene97682 | 0.833607 | 4.84E-56 | -1.69E-46 |
| MS.gene059943 | MS.gene98501 | 0.806009 | 1.01E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059943.t1 | MTR_4g104700 | 98.544 | 206 | 3 | 0 | 1 | 206 | 1 | 206 | 8.42e-154 | 424 |
| MS.gene059943.t1 | MTR_4g104730 | 98.058 | 206 | 4 | 0 | 1 | 206 | 1 | 206 | 2.36e-153 | 423 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059943.t1 | AT5G50200 | 51.675 | 209 | 90 | 5 | 1 | 202 | 1 | 205 | 2.09e-66 | 203 |
| MS.gene059943.t1 | AT5G50200 | 51.675 | 209 | 90 | 5 | 1 | 202 | 1 | 205 | 2.09e-66 | 203 |
| MS.gene059943.t1 | AT5G50200 | 51.675 | 209 | 90 | 5 | 1 | 202 | 1 | 205 | 2.09e-66 | 203 |
| MS.gene059943.t1 | AT4G24715 | 50.242 | 207 | 95 | 5 | 1 | 202 | 23 | 226 | 9.23e-65 | 200 |
Find 50 sgRNAs with CRISPR-Local
Find 76 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTAGCACAACTTCAGCTTTA+TGG | 0.134503 | 8.1:-19802610 | None:intergenic |
| ATCTACGCAGTTCTGTTATC+TGG | 0.302271 | 8.1:+19802516 | MS.gene059943:intron |
| ACTTACCTTGTCCCTGCTTA+GGG | 0.312527 | 8.1:-19801965 | None:intergenic |
| TACTTACCTTGTCCCTGCTT+AGG | 0.355450 | 8.1:-19801966 | None:intergenic |
| TGTTTAGCTGAACTTTGTTA+TGG | 0.379387 | 8.1:+19801894 | MS.gene059943:CDS |
| TCTTACCCGCGTTCGTGGTT+TGG | 0.381755 | 8.1:-19802848 | None:intergenic |
| ACTGAATTCAATTACTGCTT+TGG | 0.387524 | 8.1:-19803005 | None:intergenic |
| AACGATAAGGAAGTGGCTTA+TGG | 0.411170 | 8.1:+19802825 | MS.gene059943:CDS |
| TAGGGGAAGCTGTGACTTCA+AGG | 0.413086 | 8.1:-19801947 | None:intergenic |
| TGCATTTGATTCCAACGATA+AGG | 0.421816 | 8.1:+19802812 | MS.gene059943:CDS |
| GATTCAGCGCGACGTGCCTC+AGG | 0.452711 | 8.1:+19802767 | MS.gene059943:CDS |
| GCTTTCTCAGTTGTGTCCCT+AGG | 0.459425 | 8.1:+19802945 | MS.gene059943:CDS |
| AGTCACAGCTTCCCCTAAGC+AGG | 0.463378 | 8.1:+19801953 | MS.gene059943:CDS |
| GGTACTCTTACCCGCGTTCG+TGG | 0.474972 | 8.1:-19802853 | None:intergenic |
| AGCTGAACTTTGTTATGGAA+AGG | 0.496120 | 8.1:+19801899 | MS.gene059943:CDS |
| ATAAGCCACTTCCTTATCGT+TGG | 0.499427 | 8.1:-19802823 | None:intergenic |
| AGACCGAGGATGAACTCTCT+AGG | 0.513840 | 8.1:+19802667 | MS.gene059943:CDS |
| TATGATGAGTCTGTTCCAGC+TGG | 0.517572 | 8.1:-19802582 | None:intergenic |
| TGATTCCAACGATAAGGAAG+TGG | 0.521314 | 8.1:+19802818 | MS.gene059943:CDS |
| CTAAACAAGACCTTCCCAGC+TGG | 0.523958 | 8.1:+19802567 | MS.gene059943:CDS |
| GTTGGAAGCATTGTAGGGCA+TGG | 0.526614 | 8.1:-19802718 | None:intergenic |
| TGAGTCTGTTCCAGCTGGGA+AGG | 0.539300 | 8.1:-19802577 | None:intergenic |
| TGCGTTGATCTCAAACAAGT+TGG | 0.540469 | 8.1:-19802874 | None:intergenic |
| CTACAATGCTTCCAACAAAA+CGG | 0.541838 | 8.1:+19802725 | MS.gene059943:CDS |
| GTCCCTAGAGAGTTCATCCT+CGG | 0.558273 | 8.1:-19802670 | None:intergenic |
| TAGACAAAATCAGTGGAACA+TGG | 0.559795 | 8.1:+19802541 | MS.gene059943:CDS |
| GACCGAGGATGAACTCTCTA+GGG | 0.561011 | 8.1:+19802668 | MS.gene059943:CDS |
| AGGGGAAGCTGTGACTTCAA+GGG | 0.577454 | 8.1:-19801946 | None:intergenic |
| CTTACCTTGTCCCTGCTTAG+GGG | 0.578473 | 8.1:-19801964 | None:intergenic |
| CAATATAGAAGAACACACCT+AGG | 0.601286 | 8.1:-19802962 | None:intergenic |
| TTTGAGATCAACGCAATCAG+TGG | 0.604199 | 8.1:+19802882 | MS.gene059943:CDS |
| AGACAAAATCAGTGGAACAT+GGG | 0.618150 | 8.1:+19802542 | MS.gene059943:CDS |
| ATCGTTGGAATCAAATGCAT+AGG | 0.623735 | 8.1:-19802808 | None:intergenic |
| GTCACAGCTTCCCCTAAGCA+GGG | 0.629784 | 8.1:+19801954 | MS.gene059943:CDS |
| TATGGCCAAACCACGAACGC+GGG | 0.634750 | 8.1:+19802843 | MS.gene059943:CDS |
| TTATGGCCAAACCACGAACG+CGG | 0.634987 | 8.1:+19802842 | MS.gene059943:CDS |
| GCTTCCCCTAAGCAGGGACA+AGG | 0.636634 | 8.1:+19801960 | MS.gene059943:CDS |
| GCGCTGAATCAACCACTCGA+AGG | 0.649799 | 8.1:-19802754 | None:intergenic |
| CCTCGGTCTTTCTCCAAGCG+CGG | 0.651070 | 8.1:-19802653 | None:intergenic |
| AATATAGAAGAACACACCTA+GGG | 0.653049 | 8.1:-19802961 | None:intergenic |
| TATGGCAGCACACAAGCTTG+TGG | 0.654698 | 8.1:+19801851 | None:intergenic |
| TCTGGAGTAGACAAAATCAG+TGG | 0.660740 | 8.1:+19802534 | MS.gene059943:CDS |
| TGAGAAAGCACTGAAGACAA+CGG | 0.661115 | 8.1:-19802931 | None:intergenic |
| AAACGGTTCAAACCTTCGAG+TGG | 0.663100 | 8.1:+19802742 | MS.gene059943:CDS |
| CGTACAAAGTACGTTGCCTG+AGG | 0.678441 | 8.1:-19802783 | None:intergenic |
| AGCAGATATCAAGAGTCGCG+TGG | 0.681698 | 8.1:-19802908 | None:intergenic |
| ATGATGAGTCTGTTCCAGCT+GGG | 0.682275 | 8.1:-19802581 | None:intergenic |
| AGATATCAAGAGTCGCGTGG+CGG | 0.700580 | 8.1:-19802905 | None:intergenic |
| CCGCGCTTGGAGAAAGACCG+AGG | 0.703551 | 8.1:+19802653 | MS.gene059943:CDS |
| AAAGCTGAAGTTGTGCTACG+CGG | 0.733243 | 8.1:+19802614 | MS.gene059943:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GATAAGACATTTATTTATTA+AGG | - | chr8.1:19802406-19802425 | None:intergenic | 15.0% |
| !! | TGTTCTTCTATATTGAAAAA+AGG | + | chr8.1:19802970-19802989 | MS.gene059943:CDS | 20.0% |
| !! | TTATTCAAATTCAAGAAAAG+AGG | - | chr8.1:19802200-19802219 | None:intergenic | 20.0% |
| !! | TTCTATATTGAAAAAAGGAA+GGG | + | chr8.1:19802975-19802994 | MS.gene059943:CDS | 20.0% |
| !!! | GAACATTTTTCAAGAAATTT+TGG | + | chr8.1:19802236-19802255 | MS.gene059943:intron | 20.0% |
| ! | ATAATCACATAAATCAAGGT+TGG | - | chr8.1:19802170-19802189 | None:intergenic | 25.0% |
| ! | CAAGTATGCAATCATAATAT+AGG | - | chr8.1:19802428-19802447 | None:intergenic | 25.0% |
| ! | CTTCTATATTGAAAAAAGGA+AGG | + | chr8.1:19802974-19802993 | MS.gene059943:CDS | 25.0% |
| ! | GAACATAATCACATAAATCA+AGG | - | chr8.1:19802174-19802193 | None:intergenic | 25.0% |
| ! | TACATTACAAATGATCACAA+AGG | + | chr8.1:19802330-19802349 | MS.gene059943:intron | 25.0% |
| ! | TTCTTGAAAAATGTTCATAC+TGG | - | chr8.1:19802232-19802251 | None:intergenic | 25.0% |
| !! | AAGCTGATTAAACTCAATTT+TGG | + | chr8.1:19802485-19802504 | MS.gene059943:intron | 25.0% |
| AATATAGAAGAACACACCTA+GGG | - | chr8.1:19802964-19802983 | None:intergenic | 30.0% | |
| ATTGAGTTTAATCAGCTTGA+AGG | - | chr8.1:19802483-19802502 | None:intergenic | 30.0% | |
| CTTAATAAATAGTAACGCAC+CGG | - | chr8.1:19802278-19802297 | None:intergenic | 30.0% | |
| TCAAATTCAAGAAAAGAGGA+AGG | - | chr8.1:19802196-19802215 | None:intergenic | 30.0% | |
| TGTTTAGCTGAACTTTGTTA+TGG | + | chr8.1:19801894-19801913 | MS.gene059943:CDS | 30.0% | |
| !!! | TTTTTCAAGAAATTTTGGCC+AGG | + | chr8.1:19802241-19802260 | MS.gene059943:intron | 30.0% |
| AGACAAAATCAGTGGAACAT+GGG | + | chr8.1:19802542-19802561 | MS.gene059943:CDS | 35.0% | |
| ATCGTTGGAATCAAATGCAT+AGG | - | chr8.1:19802811-19802830 | None:intergenic | 35.0% | |
| CAATATAGAAGAACACACCT+AGG | - | chr8.1:19802965-19802984 | None:intergenic | 35.0% | |
| CGGTGCGTTACTATTTATTA+AGG | + | chr8.1:19802276-19802295 | MS.gene059943:intron | 35.0% | |
| CTACAATGCTTCCAACAAAA+CGG | + | chr8.1:19802725-19802744 | MS.gene059943:CDS | 35.0% | |
| TAGACAAAATCAGTGGAACA+TGG | + | chr8.1:19802541-19802560 | MS.gene059943:CDS | 35.0% | |
| TGCATTTGATTCCAACGATA+AGG | + | chr8.1:19802812-19802831 | MS.gene059943:CDS | 35.0% | |
| ! | AGCTGAACTTTGTTATGGAA+AGG | + | chr8.1:19801899-19801918 | MS.gene059943:CDS | 35.0% |
| !! | GAAATTTTGGCCAGGTTTAA+TGG | + | chr8.1:19802249-19802268 | MS.gene059943:intron | 35.0% |
| !!! | GTTTTGTTGGAAGCATTGTA+GGG | - | chr8.1:19802726-19802745 | None:intergenic | 35.0% |
| AAAAATTGCAACGCGACTTG+CGG | - | chr8.1:19802310-19802329 | None:intergenic | 40.0% | |
| AACGATAAGGAAGTGGCTTA+TGG | + | chr8.1:19802825-19802844 | MS.gene059943:CDS | 40.0% | |
| ATAAGCCACTTCCTTATCGT+TGG | - | chr8.1:19802826-19802845 | None:intergenic | 40.0% | |
| ATCTACGCAGTTCTGTTATC+TGG | + | chr8.1:19802516-19802535 | MS.gene059943:intron | 40.0% | |
| GTAGCACAACTTCAGCTTTA+TGG | - | chr8.1:19802613-19802632 | None:intergenic | 40.0% | |
| TGATTCCAACGATAAGGAAG+TGG | + | chr8.1:19802818-19802837 | MS.gene059943:CDS | 40.0% | |
| ! | GAAGGTTTGAACCGTTTTGT+TGG | - | chr8.1:19802739-19802758 | None:intergenic | 40.0% |
| ! | GCATGGCTAACATTTTGTGT+TGG | - | chr8.1:19802704-19802723 | None:intergenic | 40.0% |
| ! | TCTGGAGTAGACAAAATCAG+TGG | + | chr8.1:19802534-19802553 | MS.gene059943:CDS | 40.0% |
| ! | TGCGTTGATCTCAAACAAGT+TGG | - | chr8.1:19802877-19802896 | None:intergenic | 40.0% |
| ! | TTTGAGATCAACGCAATCAG+TGG | + | chr8.1:19802882-19802901 | MS.gene059943:CDS | 40.0% |
| !! | TGAGAAAGCACTGAAGACAA+CGG | - | chr8.1:19802934-19802953 | None:intergenic | 40.0% |
| !!! | CGTTTTGTTGGAAGCATTGT+AGG | - | chr8.1:19802727-19802746 | None:intergenic | 40.0% |
| AAACGGTTCAAACCTTCGAG+TGG | + | chr8.1:19802742-19802761 | MS.gene059943:CDS | 45.0% | |
| AAAGCTGAAGTTGTGCTACG+CGG | + | chr8.1:19802614-19802633 | MS.gene059943:CDS | 45.0% | |
| ACTTACCTTGTCCCTGCTTA+GGG | - | chr8.1:19801968-19801987 | None:intergenic | 45.0% | |
| ATGATGAGTCTGTTCCAGCT+GGG | - | chr8.1:19802584-19802603 | None:intergenic | 45.0% | |
| TACTTACCTTGTCCCTGCTT+AGG | - | chr8.1:19801969-19801988 | None:intergenic | 45.0% | |
| TATGATGAGTCTGTTCCAGC+TGG | - | chr8.1:19802585-19802604 | None:intergenic | 45.0% | |
| AGACCGAGGATGAACTCTCT+AGG | + | chr8.1:19802667-19802686 | MS.gene059943:CDS | 50.0% | |
| AGATATCAAGAGTCGCGTGG+CGG | - | chr8.1:19802908-19802927 | None:intergenic | 50.0% | |
| AGCAGATATCAAGAGTCGCG+TGG | - | chr8.1:19802911-19802930 | None:intergenic | 50.0% | |
| AGGGGAAGCTGTGACTTCAA+GGG | - | chr8.1:19801949-19801968 | None:intergenic | 50.0% | |
| CGTACAAAGTACGTTGCCTG+AGG | - | chr8.1:19802786-19802805 | None:intergenic | 50.0% | |
| CTAAACAAGACCTTCCCAGC+TGG | + | chr8.1:19802567-19802586 | MS.gene059943:CDS | 50.0% | |
| CTTACCTTGTCCCTGCTTAG+GGG | - | chr8.1:19801967-19801986 | None:intergenic | 50.0% | |
| GACCGAGGATGAACTCTCTA+GGG | + | chr8.1:19802668-19802687 | MS.gene059943:CDS | 50.0% | |
| GCTTTCTCAGTTGTGTCCCT+AGG | + | chr8.1:19802945-19802964 | MS.gene059943:CDS | 50.0% | |
| GTCCCTAGAGAGTTCATCCT+CGG | - | chr8.1:19802673-19802692 | None:intergenic | 50.0% | |
| TAAGCCAAAAAGACCGCGCT+TGG | + | chr8.1:19802640-19802659 | MS.gene059943:CDS | 50.0% | |
| TAGGGGAAGCTGTGACTTCA+AGG | - | chr8.1:19801950-19801969 | None:intergenic | 50.0% | |
| TGGCCAGGTTTAATGGAGTC+CGG | + | chr8.1:19802256-19802275 | MS.gene059943:intron | 50.0% | |
| TTATGGCCAAACCACGAACG+CGG | + | chr8.1:19802842-19802861 | MS.gene059943:CDS | 50.0% | |
| ! | TTCTCCAAGCGCGGTCTTTT+TGG | - | chr8.1:19802647-19802666 | None:intergenic | 50.0% |
| !! | GTTGGAAGCATTGTAGGGCA+TGG | - | chr8.1:19802721-19802740 | None:intergenic | 50.0% |
| !!! | AGCGCGGTCTTTTTGGCTTA+TGG | - | chr8.1:19802640-19802659 | None:intergenic | 50.0% |
| AGTCACAGCTTCCCCTAAGC+AGG | + | chr8.1:19801953-19801972 | MS.gene059943:CDS | 55.0% | |
| GCACCGGACTCCATTAAACC+TGG | - | chr8.1:19802262-19802281 | None:intergenic | 55.0% | |
| GCGCTGAATCAACCACTCGA+AGG | - | chr8.1:19802757-19802776 | None:intergenic | 55.0% | |
| GTCACAGCTTCCCCTAAGCA+GGG | + | chr8.1:19801954-19801973 | MS.gene059943:CDS | 55.0% | |
| TATGGCCAAACCACGAACGC+GGG | + | chr8.1:19802843-19802862 | MS.gene059943:CDS | 55.0% | |
| TCTTACCCGCGTTCGTGGTT+TGG | - | chr8.1:19802851-19802870 | None:intergenic | 55.0% | |
| TGAGTCTGTTCCAGCTGGGA+AGG | - | chr8.1:19802580-19802599 | None:intergenic | 55.0% | |
| CCTCGGTCTTTCTCCAAGCG+CGG | - | chr8.1:19802656-19802675 | None:intergenic | 60.0% | |
| GCTTCCCCTAAGCAGGGACA+AGG | + | chr8.1:19801960-19801979 | MS.gene059943:CDS | 60.0% | |
| GGTACTCTTACCCGCGTTCG+TGG | - | chr8.1:19802856-19802875 | None:intergenic | 60.0% | |
| CCGCGCTTGGAGAAAGACCG+AGG | + | chr8.1:19802653-19802672 | MS.gene059943:CDS | 65.0% | |
| GATTCAGCGCGACGTGCCTC+AGG | + | chr8.1:19802767-19802786 | MS.gene059943:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 19801852 | 19803016 | 19801852 | ID=MS.gene059943 |
| chr8.1 | mRNA | 19801852 | 19803016 | 19801852 | ID=MS.gene059943.t1;Parent=MS.gene059943 |
| chr8.1 | exon | 19801852 | 19801981 | 19801852 | ID=MS.gene059943.t1.exon1;Parent=MS.gene059943.t1 |
| chr8.1 | CDS | 19801852 | 19801981 | 19801852 | ID=cds.MS.gene059943.t1;Parent=MS.gene059943.t1 |
| chr8.1 | exon | 19802526 | 19803016 | 19802526 | ID=MS.gene059943.t1.exon2;Parent=MS.gene059943.t1 |
| chr8.1 | CDS | 19802526 | 19803016 | 19802526 | ID=cds.MS.gene059943.t1;Parent=MS.gene059943.t1 |
| Gene Sequence |
| Protein sequence |
