Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene072544.t1 | XP_003626174.1 | 93.5 | 46 | 3 | 0 | 1 | 46 | 1 | 46 | 7.60E-19 | 102.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene072544.t1 | Q8LC59 | 65.6 | 32 | 10 | 1 | 15 | 46 | 20 | 50 | 1.7e-06 | 53.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene072544.t1 | G7KWI0 | 93.5 | 46 | 3 | 0 | 1 | 46 | 1 | 46 | 5.5e-19 | 102.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene072544.t1 | TF | C2C2-GATA |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049821 | MS.gene072544 | 0.811475 | 6.87E-51 | -1.69E-46 |
| MS.gene052519 | MS.gene072544 | 0.802248 | 6.14E-49 | -1.69E-46 |
| MS.gene05415 | MS.gene072544 | 0.825289 | 5.08E-54 | -1.69E-46 |
| MS.gene055818 | MS.gene072544 | 0.807251 | 5.54E-50 | -1.69E-46 |
| MS.gene057060 | MS.gene072544 | 0.822267 | 2.60E-53 | -1.69E-46 |
| MS.gene057208 | MS.gene072544 | 0.808413 | 3.14E-50 | -1.69E-46 |
| MS.gene057693 | MS.gene072544 | 0.801146 | 1.03E-48 | -1.69E-46 |
| MS.gene060365 | MS.gene072544 | 0.808009 | 3.82E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene072544.t1 | MTR_7g112330 | 93.478 | 46 | 3 | 0 | 1 | 46 | 1 | 46 | 6.19e-28 | 97.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 17 sgRNAs with CRISPR-Local
Find 112 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCCTCTCCAAAGTGGTGTTT+TGG | 0.317787 | 7.3:+4271929 | None:intergenic |
| AATGATACCTTGGGTCCATT+TGG | 0.368205 | 7.3:+4271903 | None:intergenic |
| AATGGGAATCATGGATCAAA+TGG | 0.411033 | 7.3:-4272133 | MS.gene072544:CDS |
| ATGTTGTTCATACGATGAAA+TGG | 0.428480 | 7.3:-4271988 | MS.gene072544:CDS |
| AATCATGGATCAAATGGATA+AGG | 0.481862 | 7.3:-4272127 | MS.gene072544:intron |
| CTTTGGAGAGGAGGACCAAA+TGG | 0.503109 | 7.3:-4271918 | MS.gene072544:CDS |
| TGTGAATCAAATGGGAATCA+TGG | 0.514991 | 7.3:-4272142 | None:intergenic |
| AAACCACCAAAACACCACTT+TGG | 0.542523 | 7.3:-4271935 | MS.gene072544:CDS |
| TGTTGTTCATACGATGAAAT+GGG | 0.559954 | 7.3:-4271987 | MS.gene072544:CDS |
| AGAATGAGATTAAATTGTCT+AGG | 0.578156 | 7.3:+4270858 | None:intergenic |
| ACATCGTAGCAGTAAGCACT+AGG | 0.588556 | 7.3:+4270819 | None:intergenic |
| CACTAGGTGGACGATTGTAG+TGG | 0.592815 | 7.3:+4270835 | None:intergenic |
| TTTGGTCCTCCTCTCCAAAG+TGG | 0.621363 | 7.3:+4271921 | None:intergenic |
| AAAACACCACTTTGGAGAGG+AGG | 0.625709 | 7.3:-4271927 | MS.gene072544:CDS |
| AGGAGGACCAAATGGACCCA+AGG | 0.633971 | 7.3:-4271910 | MS.gene072544:intron |
| ACCAAAACACCACTTTGGAG+AGG | 0.669357 | 7.3:-4271930 | MS.gene072544:CDS |
| TCGTAGCAGTAAGCACTAGG+TGG | 0.701990 | 7.3:+4270822 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GAAATAAAATAAAAAAGAAA+AGG | + | chr7.3:4271883-4271902 | None:intergenic | 10.0% |
| !!! | ATATTTTTTTTTGTTTTTGT+TGG | - | chr7.3:4271277-4271296 | MS.gene072544:intron | 10.0% |
| !!! | ATGTTTATTAATTTGATTAA+AGG | - | chr7.3:4271219-4271238 | MS.gene072544:intron | 10.0% |
| !!! | TATTTTTTTTTGTTTTTGTT+GGG | - | chr7.3:4271278-4271297 | MS.gene072544:intron | 10.0% |
| !!! | TGTTTATTAATTTGATTAAA+GGG | - | chr7.3:4271220-4271239 | MS.gene072544:intron | 10.0% |
| !! | AAAAAAAAAAAATGATACCT+TGG | + | chr7.3:4271052-4271071 | None:intergenic | 15.0% |
| !! | AAAAAAAAAAATGATACCTT+GGG | + | chr7.3:4271051-4271070 | None:intergenic | 15.0% |
| !! | AAACAATAATCATATCAAAA+AGG | + | chr7.3:4271184-4271203 | None:intergenic | 15.0% |
| !! | AAGTGAATAATATATCAAAT+TGG | + | chr7.3:4271155-4271174 | None:intergenic | 15.0% |
| !! | AATATTAAAATGTTAGAAGA+AGG | - | chr7.3:4271622-4271641 | MS.gene072544:intron | 15.0% |
| !! | ATATTAAAATGTTAGAAGAA+GGG | - | chr7.3:4271623-4271642 | MS.gene072544:intron | 15.0% |
| !!! | GTTTTCAAATATAGATAAAA+TGG | + | chr7.3:4271127-4271146 | None:intergenic | 15.0% |
| !! | AAGTATTTCACATTTATGAA+TGG | - | chr7.3:4271962-4271981 | MS.gene072544:CDS | 20.0% |
| !! | AAGTTACAAAATGATGAAAT+GGG | + | chr7.3:4271078-4271097 | None:intergenic | 20.0% |
| !! | AGCATAGATATATAAACAAA+AGG | + | chr7.3:4271906-4271925 | None:intergenic | 20.0% |
| !! | AGTATTTCACATTTATGAAT+GGG | - | chr7.3:4271963-4271982 | MS.gene072544:CDS | 20.0% |
| !! | GCATAGATATATAAACAAAA+GGG | + | chr7.3:4271905-4271924 | None:intergenic | 20.0% |
| !! | TAAGTTACAAAATGATGAAA+TGG | + | chr7.3:4271079-4271098 | None:intergenic | 20.0% |
| !! | TATTAAAATGTTAGAAGAAG+GGG | - | chr7.3:4271624-4271643 | MS.gene072544:intron | 20.0% |
| !! | TGAATAATATATCAAATTGG+TGG | + | chr7.3:4271152-4271171 | None:intergenic | 20.0% |
| !!! | CTTTCTTTAGTTTTTAGAAA+GGG | - | chr7.3:4271781-4271800 | MS.gene072544:intron | 20.0% |
| !!! | TGATTGTTATTATTGTTCTT+TGG | - | chr7.3:4271987-4272006 | MS.gene072544:CDS | 20.0% |
| ! | AAACAAAGAAGGATTAAACT+AGG | - | chr7.3:4271525-4271544 | MS.gene072544:intron | 25.0% |
| ! | AAAGAGAGAAAACAAAAAAG+AGG | + | chr7.3:4271728-4271747 | None:intergenic | 25.0% |
| ! | AGAATGAGATTAAATTGTCT+AGG | + | chr7.3:4272087-4272106 | None:intergenic | 25.0% |
| ! | AGATAAAATGGAAGTGAAAA+AGG | + | chr7.3:4271115-4271134 | None:intergenic | 25.0% |
| ! | ATTAAAATGTTAGAAGAAGG+GGG | - | chr7.3:4271625-4271644 | MS.gene072544:intron | 25.0% |
| ! | CTTCTTTCTATTTGTTTAAC+AGG | - | chr7.3:4270930-4270949 | MS.gene072544:intron | 25.0% |
| ! | GTATTTCACATTTATGAATG+GGG | - | chr7.3:4271964-4271983 | MS.gene072544:CDS | 25.0% |
| !! | CCTTTCTAAAAACTAAAGAA+AGG | + | chr7.3:4271783-4271802 | None:intergenic | 25.0% |
| !!! | CCTTTCTTTAGTTTTTAGAA+AGG | - | chr7.3:4271780-4271799 | MS.gene072544:intron | 25.0% |
| !!! | TTTTGTTTTCTCTCTTTCTT+TGG | - | chr7.3:4271731-4271750 | MS.gene072544:intron | 25.0% |
| AAAATGAAGTTAGTGGCATT+GGG | - | chr7.3:4271480-4271499 | MS.gene072544:intron | 30.0% | |
| AATCATGGATCAAATGGATA+AGG | - | chr7.3:4270815-4270834 | MS.gene072544:CDS | 30.0% | |
| ACTTAGTTCCAAATCTTGTT+AGG | - | chr7.3:4271816-4271835 | MS.gene072544:intron | 30.0% | |
| ATGTTGTTCATACGATGAAA+TGG | - | chr7.3:4270954-4270973 | MS.gene072544:intron | 30.0% | |
| CTTAGTTCCAAATCTTGTTA+GGG | - | chr7.3:4271817-4271836 | MS.gene072544:intron | 30.0% | |
| GTGTTTGAAAATGAAGTTAG+TGG | - | chr7.3:4271473-4271492 | MS.gene072544:intron | 30.0% | |
| TGTTGTTCATACGATGAAAT+GGG | - | chr7.3:4270955-4270974 | MS.gene072544:intron | 30.0% | |
| TTAAAATGTTAGAAGAAGGG+GGG | - | chr7.3:4271626-4271645 | MS.gene072544:intron | 30.0% | |
| ! | TTCTATCCTTTTAGACTCTA+GGG | - | chr7.3:4272015-4272034 | MS.gene072544:intron | 30.0% |
| ! | TTTCTATCCTTTTAGACTCT+AGG | - | chr7.3:4272014-4272033 | MS.gene072544:intron | 30.0% |
| AAGGATTAAACTAGGTGAAG+AGG | - | chr7.3:4271533-4271552 | MS.gene072544:intron | 35.0% | |
| AATCAGGTACAGAAAGAGAA+GGG | - | chr7.3:4271329-4271348 | MS.gene072544:intron | 35.0% | |
| ACTAATAACTAACTGAACCG+AGG | + | chr7.3:4271940-4271959 | None:intergenic | 35.0% | |
| CAAAAACCCTAGAGTCTAAA+AGG | + | chr7.3:4272024-4272043 | None:intergenic | 35.0% | |
| GAAAATGAAGTTAGTGGCAT+TGG | - | chr7.3:4271479-4271498 | MS.gene072544:intron | 35.0% | |
| TAAAATGTTAGAAGAAGGGG+GGG | - | chr7.3:4271627-4271646 | MS.gene072544:intron | 35.0% | |
| TCGTCCAACAAAAAAGAATC+CGG | + | chr7.3:4272058-4272077 | None:intergenic | 35.0% | |
| TGTTGCAGAAGAAACAAAGA+AGG | - | chr7.3:4271514-4271533 | MS.gene072544:intron | 35.0% | |
| TTGTAGCAAAGGACAAGAAA+GGG | - | chr7.3:4271356-4271375 | MS.gene072544:intron | 35.0% | |
| TTTCTTTGAGCTTACTAGCT+AGG | - | chr7.3:4270852-4270871 | MS.gene072544:CDS | 35.0% | |
| !! | TATGCTAATTTGTTCAGCCT+CGG | - | chr7.3:4271920-4271939 | MS.gene072544:CDS | 35.0% |
| !!! | AAAGAAAAGGCACTCCTTTT+TGG | + | chr7.3:4271870-4271889 | None:intergenic | 35.0% |
| !!! | ATCTCCGGATTCTTTTTTGT+TGG | - | chr7.3:4272051-4272070 | MS.gene072544:intron | 35.0% |
| !!! | GGTTTTTGTTGTTCAATCTC+CGG | - | chr7.3:4272036-4272055 | MS.gene072544:intron | 35.0% |
| AAAATGTTAGAAGAAGGGGG+GGG | - | chr7.3:4271628-4271647 | MS.gene072544:intron | 40.0% | |
| AAACCACCAAAACACCACTT+TGG | - | chr7.3:4271007-4271026 | MS.gene072544:intron | 40.0% | |
| AATGATACCTTGGGTCCATT+TGG | + | chr7.3:4271042-4271061 | None:intergenic | 40.0% | |
| ATCAGGTACAGAAAGAGAAG+GGG | - | chr7.3:4271330-4271349 | MS.gene072544:intron | 40.0% | |
| CAAAGGACAAGAAAGGGAAA+GGG | - | chr7.3:4271362-4271381 | MS.gene072544:intron | 40.0% | |
| CCAAATCTTGTTAGGGACTA+GGG | - | chr7.3:4271824-4271843 | MS.gene072544:intron | 40.0% | |
| GAATCAGGTACAGAAAGAGA+AGG | - | chr7.3:4271328-4271347 | MS.gene072544:intron | 40.0% | |
| GTTGTAGCAAAGGACAAGAA+AGG | - | chr7.3:4271355-4271374 | MS.gene072544:intron | 40.0% | |
| TCCAAATCTTGTTAGGGACT+AGG | - | chr7.3:4271823-4271842 | MS.gene072544:intron | 40.0% | |
| ! | CACTCCTTTTTGGATTTGGA+TGG | + | chr7.3:4271860-4271879 | None:intergenic | 40.0% |
| !! | AAGGCACTCCTTTTTGGATT+TGG | + | chr7.3:4271864-4271883 | None:intergenic | 40.0% |
| AAATGTTAGAAGAAGGGGGG+GGG | - | chr7.3:4271629-4271648 | MS.gene072544:intron | 45.0% | |
| AAGGTGCACAGAATGGGAAT+GGG | - | chr7.3:4271400-4271419 | MS.gene072544:intron | 45.0% | |
| ACAAGCTGCTGTGTGTTTGA+TGG | - | chr7.3:4271557-4271576 | MS.gene072544:intron | 45.0% | |
| ACATCGTAGCAGTAAGCACT+AGG | + | chr7.3:4272126-4272145 | None:intergenic | 45.0% | |
| AGAAGGGGTTGTTGTAGCAA+AGG | - | chr7.3:4271345-4271364 | MS.gene072544:intron | 45.0% | |
| AGGGAAAGGAAGAGAGAGAA+AGG | - | chr7.3:4271381-4271400 | MS.gene072544:intron | 45.0% | |
| CCCTAGTCCCTAACAAGATT+TGG | + | chr7.3:4271827-4271846 | None:intergenic | 45.0% | |
| GACAAGAAAGGGAAAGGGAA+AGG | - | chr7.3:4271367-4271386 | MS.gene072544:intron | 45.0% | |
| GCAAAGGACAAGAAAGGGAA+AGG | - | chr7.3:4271361-4271380 | MS.gene072544:intron | 45.0% | |
| GTGGCCATCCAAATCCAAAA+AGG | - | chr7.3:4271853-4271872 | MS.gene072544:intron | 45.0% | |
| ! | AAAACACCACTTTGGAGAGG+AGG | - | chr7.3:4271015-4271034 | MS.gene072544:intron | 45.0% |
| ! | AAAGGTGCACAGAATGGGAA+TGG | - | chr7.3:4271399-4271418 | MS.gene072544:intron | 45.0% |
| ! | ACCAAAACACCACTTTGGAG+AGG | - | chr7.3:4271012-4271031 | MS.gene072544:intron | 45.0% |
| ! | AGAGAGAAAGGTGCACAGAA+TGG | - | chr7.3:4271393-4271412 | MS.gene072544:intron | 45.0% |
| ! | GAGAGAAAGGTGCACAGAAT+GGG | - | chr7.3:4271394-4271413 | MS.gene072544:intron | 45.0% |
| ! | TCCTCTCCAAAGTGGTGTTT+TGG | + | chr7.3:4271016-4271035 | None:intergenic | 45.0% |
| ! | TGTTTGATGGCACTCTCTTG+TGG | - | chr7.3:4271570-4271589 | MS.gene072544:intron | 45.0% |
| !! | TCTCCAAAGTGGTGTTTTGG+TGG | + | chr7.3:4271013-4271032 | None:intergenic | 45.0% |
| AATGTTAGAAGAAGGGGGGG+GGG | - | chr7.3:4271630-4271649 | MS.gene072544:intron | 50.0% | |
| CACTAGGTGGACGATTGTAG+TGG | + | chr7.3:4272110-4272129 | None:intergenic | 50.0% | |
| TAGACGTTGTGCAACGCTTG+TGG | - | chr7.3:4271306-4271325 | MS.gene072544:intron | 50.0% | |
| TCGTAGCAGTAAGCACTAGG+TGG | + | chr7.3:4272123-4272142 | None:intergenic | 50.0% | |
| TGCACAGAATGGGAATGGGA+GGG | - | chr7.3:4271404-4271423 | MS.gene072544:intron | 50.0% | |
| TGTGCAACGCTTGTGGAATC+AGG | - | chr7.3:4271313-4271332 | MS.gene072544:intron | 50.0% | |
| TTGTTAGGGACTAGGGAGTG+AGG | - | chr7.3:4271831-4271850 | MS.gene072544:intron | 50.0% | |
| TTTGGTCCTCCTCTCCAAAG+TGG | + | chr7.3:4271024-4271043 | None:intergenic | 50.0% | |
| ! | CTTTGGAGAGGAGGACCAAA+TGG | - | chr7.3:4271024-4271043 | MS.gene072544:intron | 50.0% |
| AGGAGGACCAAATGGACCCA+AGG | - | chr7.3:4271032-4271051 | MS.gene072544:intron | 55.0% | |
| ATGTTAGAAGAAGGGGGGGG+GGG | - | chr7.3:4271631-4271650 | MS.gene072544:intron | 55.0% | |
| GCACAGAATGGGAATGGGAG+GGG | - | chr7.3:4271405-4271424 | MS.gene072544:intron | 55.0% | |
| GGTGGTACTAGCAGTAGCAG+TGG | - | chr7.3:4271426-4271445 | MS.gene072544:intron | 55.0% | |
| GTGCACAGAATGGGAATGGG+AGG | - | chr7.3:4271403-4271422 | MS.gene072544:intron | 55.0% | |
| TTAGGGACTAGGGAGTGAGG+TGG | - | chr7.3:4271834-4271853 | MS.gene072544:intron | 55.0% | |
| CAGAATGGGAATGGGAGGGG+TGG | - | chr7.3:4271408-4271427 | MS.gene072544:intron | 60.0% | |
| TGTTAGAAGAAGGGGGGGGG+GGG | - | chr7.3:4271632-4271651 | MS.gene072544:intron | 60.0% | |
| GTTAGAAGAAGGGGGGGGGG+GGG | - | chr7.3:4271633-4271652 | MS.gene072544:intron | 65.0% | |
| TTAGAAGAAGGGGGGGGGGG+GGG | - | chr7.3:4271634-4271653 | MS.gene072544:intron | 65.0% | |
| TAGAAGAAGGGGGGGGGGGG+GGG | - | chr7.3:4271635-4271654 | MS.gene072544:intron | 70.0% | |
| AGAAGAAGGGGGGGGGGGGG+GGG | - | chr7.3:4271636-4271655 | MS.gene072544:intron | 75.0% | |
| ! | AAGAAGGGGGGGGGGGGGGG+GGG | - | chr7.3:4271638-4271657 | MS.gene072544:intron | 80.0% |
| ! | GAAGAAGGGGGGGGGGGGGG+GGG | - | chr7.3:4271637-4271656 | MS.gene072544:intron | 80.0% |
| ! | AGAAGGGGGGGGGGGGGGGG+GGG | - | chr7.3:4271639-4271658 | MS.gene072544:intron | 85.0% |
| ! | AAGGGGGGGGGGGGGGGGGG+GGG | - | chr7.3:4271641-4271660 | MS.gene072544:intron | 90.0% |
| ! | GAAGGGGGGGGGGGGGGGGG+GGG | - | chr7.3:4271640-4271659 | MS.gene072544:intron | 90.0% |
| ! | AGGGGGGGGGGGGGGGGGGG+GGG | - | chr7.3:4271642-4271661 | MS.gene072544:intron | 95.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 4270810 | 4272154 | 4270810 | ID=MS.gene072544 |
| chr7.3 | mRNA | 4270810 | 4272154 | 4270810 | ID=MS.gene072544.t1;Parent=MS.gene072544 |
| chr7.3 | exon | 4272128 | 4272154 | 4272128 | ID=MS.gene072544.t1.exon1;Parent=MS.gene072544.t1 |
| chr7.3 | CDS | 4272128 | 4272154 | 4272128 | ID=cds.MS.gene072544.t1;Parent=MS.gene072544.t1 |
| chr7.3 | exon | 4271911 | 4272012 | 4271911 | ID=MS.gene072544.t1.exon2;Parent=MS.gene072544.t1 |
| chr7.3 | CDS | 4271911 | 4272012 | 4271911 | ID=cds.MS.gene072544.t1;Parent=MS.gene072544.t1 |
| chr7.3 | exon | 4270810 | 4270928 | 4270810 | ID=MS.gene072544.t1.exon3;Parent=MS.gene072544.t1 |
| chr7.3 | CDS | 4270810 | 4270928 | 4270810 | ID=cds.MS.gene072544.t1;Parent=MS.gene072544.t1 |
| Gene Sequence |
| Protein sequence |
