Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07259.t1 | XP_003591454.1 | 97.3 | 185 | 5 | 0 | 1 | 185 | 1 | 185 | 3.20E-97 | 364.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07259.t1 | G7IEH4 | 97.3 | 185 | 5 | 0 | 1 | 185 | 1 | 185 | 2.3e-97 | 364.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene04998 | MS.gene07259 | 0.811956 | 5.40E-51 | -1.69E-46 |
| MS.gene051226 | MS.gene07259 | 0.835312 | 1.81E-56 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07259.t1 | MTR_1g087710 | 97.297 | 185 | 5 | 0 | 1 | 185 | 1 | 185 | 1.69e-135 | 376 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07259.t1 | AT5G38700 | 47.568 | 185 | 73 | 8 | 4 | 178 | 7 | 177 | 2.52e-42 | 140 |
| MS.gene07259.t1 | AT4G02170 | 44.444 | 180 | 72 | 8 | 3 | 178 | 9 | 164 | 1.54e-37 | 127 |
Find 33 sgRNAs with CRISPR-Local
Find 33 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGTATTGTAGAAGAGAAATT+TGG | 0.265595 | 1.3:+61958772 | None:intergenic |
| CAATCAGTTCGTGTCTCTGC+TGG | 0.266163 | 1.3:+61958740 | None:intergenic |
| CCAAAGTATTTGAAACGTTT+AGG | 0.333723 | 1.3:-61959158 | MS.gene07259:CDS |
| AAGTAGTGAATTTGATTAGT+AGG | 0.363808 | 1.3:+61959122 | None:intergenic |
| GGTTGAATTATGTGAAGAAA+TGG | 0.410470 | 1.3:-61958886 | MS.gene07259:CDS |
| CCTAAACGTTTCAAATACTT+TGG | 0.411626 | 1.3:+61959158 | None:intergenic |
| AAGGTGAAGGAATAGATAAA+CGG | 0.442626 | 1.3:-61958842 | MS.gene07259:CDS |
| ATGCTCTACGACCAACATTA+CGG | 0.446197 | 1.3:+61959264 | None:intergenic |
| CACGTAAAAGTGGTGTGTTT+AGG | 0.450583 | 1.3:-61959211 | MS.gene07259:CDS |
| GTTGAATTATGTGAAGAAAT+GGG | 0.488508 | 1.3:-61958885 | MS.gene07259:CDS |
| AATTTCTCTTCTACAATACA+AGG | 0.499143 | 1.3:-61958769 | MS.gene07259:CDS |
| AGGTGAAGGAATAGATAAAC+GGG | 0.501962 | 1.3:-61958841 | MS.gene07259:CDS |
| GTTGCGGCTCTCATTGTTGT+GGG | 0.511706 | 1.3:-61959234 | MS.gene07259:CDS |
| TTTAGAGAAGAAGTGCAAGA+AGG | 0.523132 | 1.3:-61958861 | MS.gene07259:CDS |
| ATATTGTCGAATATGACAAT+GGG | 0.524788 | 1.3:-61958962 | MS.gene07259:CDS |
| GGGTTTATACACGGAATATG+AGG | 0.528412 | 1.3:+61959020 | None:intergenic |
| AATCAGTTCGTGTCTCTGCT+GGG | 0.530386 | 1.3:+61958741 | None:intergenic |
| AAGTGAAAGCGCAACGATGA+TGG | 0.530892 | 1.3:+61959044 | None:intergenic |
| AAGAGTGATCTAGTCGATGA+AGG | 0.538924 | 1.3:-61958936 | MS.gene07259:CDS |
| GAAGAAGTGCAAGAAGGTGA+AGG | 0.541678 | 1.3:-61958855 | MS.gene07259:CDS |
| TGTTGCGGCTCTCATTGTTG+TGG | 0.565724 | 1.3:-61959235 | MS.gene07259:CDS |
| GTAATGTTGGTCGTAGAGCA+TGG | 0.569096 | 1.3:-61959262 | MS.gene07259:CDS |
| GAACATAGGTGTCTCATCAA+AGG | 0.572782 | 1.3:+61959085 | None:intergenic |
| TCATAATCAAAATCAACATG+TGG | 0.573660 | 1.3:+61958999 | None:intergenic |
| TCTTTGAAGAACCGTAATGT+TGG | 0.586513 | 1.3:-61959275 | MS.gene07259:CDS |
| CATAATCAAAATCAACATGT+GGG | 0.597783 | 1.3:+61959000 | None:intergenic |
| TTGTTGTGGGCACGTAAAAG+TGG | 0.612261 | 1.3:-61959221 | MS.gene07259:CDS |
| GCTGTAGTCTCATTTGCTGA+CGG | 0.612685 | 1.3:+61958796 | None:intergenic |
| GATATTGTCGAATATGACAA+TGG | 0.619173 | 1.3:-61958963 | MS.gene07259:CDS |
| TCAACATGTGGGTTTATACA+CGG | 0.620730 | 1.3:+61959011 | None:intergenic |
| ATTACGGTTCTTCAAAGACA+TGG | 0.628084 | 1.3:+61959280 | None:intergenic |
| GTAGAGCATGGAATATGTTG+CGG | 0.642910 | 1.3:-61959250 | MS.gene07259:CDS |
| TACATCTTGACATTGAACAT+AGG | 0.655429 | 1.3:+61959071 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAGTAGTGAATTTGATTAGT+AGG | + | chr1.3:61958902-61958921 | None:intergenic | 25.0% |
| ! | AATTTCTCTTCTACAATACA+AGG | - | chr1.3:61959252-61959271 | MS.gene07259:CDS | 25.0% |
| ! | ATATTGTCGAATATGACAAT+GGG | - | chr1.3:61959059-61959078 | MS.gene07259:CDS | 25.0% |
| ! | CATAATCAAAATCAACATGT+GGG | + | chr1.3:61959024-61959043 | None:intergenic | 25.0% |
| ! | GTTGAATTATGTGAAGAAAT+GGG | - | chr1.3:61959136-61959155 | MS.gene07259:CDS | 25.0% |
| ! | TCATAATCAAAATCAACATG+TGG | + | chr1.3:61959025-61959044 | None:intergenic | 25.0% |
| ! | TGTATTGTAGAAGAGAAATT+TGG | + | chr1.3:61959252-61959271 | None:intergenic | 25.0% |
| !! | ATGACGAAGAATTTTATCAT+GGG | - | chr1.3:61959113-61959132 | MS.gene07259:CDS | 25.0% |
| !! | TATGACGAAGAATTTTATCA+TGG | - | chr1.3:61959112-61959131 | MS.gene07259:CDS | 25.0% |
| AAGGTGAAGGAATAGATAAA+CGG | - | chr1.3:61959179-61959198 | MS.gene07259:CDS | 30.0% | |
| CCTAAACGTTTCAAATACTT+TGG | + | chr1.3:61958866-61958885 | None:intergenic | 30.0% | |
| GATATTGTCGAATATGACAA+TGG | - | chr1.3:61959058-61959077 | MS.gene07259:CDS | 30.0% | |
| GGTTGAATTATGTGAAGAAA+TGG | - | chr1.3:61959135-61959154 | MS.gene07259:CDS | 30.0% | |
| TACATCTTGACATTGAACAT+AGG | + | chr1.3:61958953-61958972 | None:intergenic | 30.0% | |
| ! | CCAAAGTATTTGAAACGTTT+AGG | - | chr1.3:61958863-61958882 | MS.gene07259:CDS | 30.0% |
| ! | TGACGAAGAATTTTATCATG+GGG | - | chr1.3:61959114-61959133 | MS.gene07259:CDS | 30.0% |
| AGGTGAAGGAATAGATAAAC+GGG | - | chr1.3:61959180-61959199 | MS.gene07259:CDS | 35.0% | |
| TCAACATGTGGGTTTATACA+CGG | + | chr1.3:61959013-61959032 | None:intergenic | 35.0% | |
| ! | TCTTTGAAGAACCGTAATGT+TGG | - | chr1.3:61958746-61958765 | MS.gene07259:CDS | 35.0% |
| ! | TTTAGAGAAGAAGTGCAAGA+AGG | - | chr1.3:61959160-61959179 | MS.gene07259:CDS | 35.0% |
| AAGAGTGATCTAGTCGATGA+AGG | - | chr1.3:61959085-61959104 | MS.gene07259:CDS | 40.0% | |
| ATGCTCTACGACCAACATTA+CGG | + | chr1.3:61958760-61958779 | None:intergenic | 40.0% | |
| CACGTAAAAGTGGTGTGTTT+AGG | - | chr1.3:61958810-61958829 | MS.gene07259:CDS | 40.0% | |
| GAACATAGGTGTCTCATCAA+AGG | + | chr1.3:61958939-61958958 | None:intergenic | 40.0% | |
| GGGTTTATACACGGAATATG+AGG | + | chr1.3:61959004-61959023 | None:intergenic | 40.0% | |
| GTAGAGCATGGAATATGTTG+CGG | - | chr1.3:61958771-61958790 | MS.gene07259:CDS | 40.0% | |
| GTAATGTTGGTCGTAGAGCA+TGG | - | chr1.3:61958759-61958778 | MS.gene07259:CDS | 45.0% | |
| TTGTTGTGGGCACGTAAAAG+TGG | - | chr1.3:61958800-61958819 | MS.gene07259:CDS | 45.0% | |
| ! | AAGTGAAAGCGCAACGATGA+TGG | + | chr1.3:61958980-61958999 | None:intergenic | 45.0% |
| ! | GAAGAAGTGCAAGAAGGTGA+AGG | - | chr1.3:61959166-61959185 | MS.gene07259:CDS | 45.0% |
| ! | GCTGTAGTCTCATTTGCTGA+CGG | + | chr1.3:61959228-61959247 | None:intergenic | 45.0% |
| GTTGCGGCTCTCATTGTTGT+GGG | - | chr1.3:61958787-61958806 | MS.gene07259:CDS | 50.0% | |
| TGTTGCGGCTCTCATTGTTG+TGG | - | chr1.3:61958786-61958805 | MS.gene07259:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.3 | gene | 61958743 | 61959300 | 61958743 | ID=MS.gene07259 |
| chr1.3 | mRNA | 61958743 | 61959300 | 61958743 | ID=MS.gene07259.t1;Parent=MS.gene07259 |
| chr1.3 | exon | 61958743 | 61959300 | 61958743 | ID=MS.gene07259.t1.exon1;Parent=MS.gene07259.t1 |
| chr1.3 | CDS | 61958743 | 61959300 | 61958743 | ID=cds.MS.gene07259.t1;Parent=MS.gene07259.t1 |
| Gene Sequence |
| Protein sequence |
