Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051226.t1 | XP_024631374.1 | 93 | 416 | 27 | 1 | 1 | 416 | 98 | 511 | 5.60E-227 | 796.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051226.t1 | Q9M2U3 | 30.4 | 322 | 193 | 9 | 76 | 374 | 53 | 366 | 4.6e-36 | 153.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051226.t1 | I3S691 | 92.5 | 416 | 29 | 1 | 1 | 416 | 98 | 511 | 7.6e-226 | 792.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene04998 | MS.gene051226 | 0.864904 | 8.69E-65 | -1.69E-46 |
| MS.gene050414 | MS.gene051226 | 0.813052 | 3.11E-51 | -1.69E-46 |
| MS.gene051043 | MS.gene051226 | 0.810971 | 8.84E-51 | -1.69E-46 |
| MS.gene051226 | MS.gene051837 | 0.865056 | 7.79E-65 | -1.69E-46 |
| MS.gene051226 | MS.gene052016 | 0.825505 | 4.52E-54 | -1.69E-46 |
| MS.gene051226 | MS.gene052296 | 0.82136 | 4.21E-53 | -1.69E-46 |
| MS.gene051226 | MS.gene053215 | 0.812068 | 5.11E-51 | -1.69E-46 |
| MS.gene051226 | MS.gene054617 | 0.84322 | 1.60E-58 | -1.69E-46 |
| MS.gene051226 | MS.gene054892 | 0.832436 | 9.46E-56 | -1.69E-46 |
| MS.gene051226 | MS.gene054893 | 0.833403 | 5.44E-56 | -1.69E-46 |
| MS.gene051226 | MS.gene057093 | 0.863598 | 2.22E-64 | -1.69E-46 |
| MS.gene051226 | MS.gene057432 | 0.835473 | 1.64E-56 | -1.69E-46 |
| MS.gene051226 | MS.gene057622 | 0.818584 | 1.82E-52 | -1.69E-46 |
| MS.gene051226 | MS.gene060964 | 0.81964 | 1.04E-52 | -1.69E-46 |
| MS.gene051226 | MS.gene061543 | 0.807242 | 5.56E-50 | -1.69E-46 |
| MS.gene051226 | MS.gene061546 | 0.82815 | 1.05E-54 | -1.69E-46 |
| MS.gene051226 | MS.gene064243 | 0.809719 | 1.65E-50 | -1.69E-46 |
| MS.gene051226 | MS.gene065073 | 0.816714 | 4.80E-52 | -1.69E-46 |
| MS.gene051226 | MS.gene067477 | 0.854819 | 9.55E-62 | -1.69E-46 |
| MS.gene051226 | MS.gene06998 | 0.815033 | 1.14E-51 | -1.69E-46 |
| MS.gene051226 | MS.gene07073 | 0.812931 | 3.31E-51 | -1.69E-46 |
| MS.gene051226 | MS.gene070918 | 0.803835 | 2.88E-49 | -1.69E-46 |
| MS.gene051226 | MS.gene072325 | 0.837803 | 4.19E-57 | -1.69E-46 |
| MS.gene051226 | MS.gene072326 | 0.832457 | 9.35E-56 | -1.69E-46 |
| MS.gene051226 | MS.gene07259 | 0.835312 | 1.81E-56 | -1.69E-46 |
| MS.gene051226 | MS.gene07267 | 0.865012 | 8.04E-65 | -1.69E-46 |
| MS.gene051226 | MS.gene07347 | 0.807977 | 3.88E-50 | -1.69E-46 |
| MS.gene051226 | MS.gene08120 | 0.812256 | 4.64E-51 | -1.69E-46 |
| MS.gene051226 | MS.gene08627 | 0.810437 | 1.15E-50 | -1.69E-46 |
| MS.gene051226 | MS.gene20064 | 0.804392 | 2.21E-49 | -1.69E-46 |
| MS.gene051226 | MS.gene20780 | 0.806094 | 9.72E-50 | -1.69E-46 |
| MS.gene051226 | MS.gene20788 | 0.868783 | 5.05E-66 | -1.69E-46 |
| MS.gene051226 | MS.gene20839 | 0.871622 | 5.94E-67 | -1.69E-46 |
| MS.gene051226 | MS.gene22433 | 0.806877 | 6.65E-50 | -1.69E-46 |
| MS.gene051226 | MS.gene22541 | 0.928602 | 2.29E-92 | -1.69E-46 |
| MS.gene051226 | MS.gene22543 | 0.926934 | 2.37E-91 | -1.69E-46 |
| MS.gene051226 | MS.gene24827 | 0.810088 | 1.37E-50 | -1.69E-46 |
| MS.gene051226 | MS.gene24855 | 0.877375 | 6.61E-69 | -1.69E-46 |
| MS.gene051226 | MS.gene24856 | 0.864508 | 1.16E-64 | -1.69E-46 |
| MS.gene051226 | MS.gene25061 | 0.827681 | 1.37E-54 | -1.69E-46 |
| MS.gene051226 | MS.gene26632 | 0.807278 | 5.46E-50 | -1.69E-46 |
| MS.gene051226 | MS.gene28023 | 0.800775 | 1.23E-48 | -1.69E-46 |
| MS.gene051226 | MS.gene28820 | 0.804233 | 2.38E-49 | -1.69E-46 |
| MS.gene051226 | MS.gene29793 | 0.813095 | 3.05E-51 | -1.69E-46 |
| MS.gene051226 | MS.gene29794 | 0.864611 | 1.07E-64 | -1.69E-46 |
| MS.gene051226 | MS.gene32688 | 0.820158 | 7.95E-53 | -1.69E-46 |
| MS.gene051226 | MS.gene32929 | 0.804226 | 2.39E-49 | -1.69E-46 |
| MS.gene051226 | MS.gene35562 | 0.842616 | 2.32E-58 | -1.69E-46 |
| MS.gene051226 | MS.gene35563 | 0.865446 | 5.87E-65 | -1.69E-46 |
| MS.gene051226 | MS.gene36866 | 0.809627 | 1.72E-50 | -1.69E-46 |
| MS.gene051226 | MS.gene36868 | 0.829354 | 5.39E-55 | -1.69E-46 |
| MS.gene051226 | MS.gene38368 | 0.812783 | 3.56E-51 | -1.69E-46 |
| MS.gene051226 | MS.gene38542 | 0.821778 | 3.37E-53 | -1.69E-46 |
| MS.gene051226 | MS.gene39645 | 0.800623 | 1.32E-48 | -1.69E-46 |
| MS.gene051226 | MS.gene41022 | 0.86039 | 2.14E-63 | -1.69E-46 |
| MS.gene051226 | MS.gene41597 | 0.824709 | 6.97E-54 | -1.69E-46 |
| MS.gene051226 | MS.gene42514 | 0.803972 | 2.70E-49 | -1.69E-46 |
| MS.gene051226 | MS.gene42516 | 0.813116 | 3.01E-51 | -1.69E-46 |
| MS.gene051226 | MS.gene42653 | 0.817429 | 3.31E-52 | -1.69E-46 |
| MS.gene051226 | MS.gene43381 | 0.83538 | 1.74E-56 | -1.69E-46 |
| MS.gene051226 | MS.gene44532 | 0.80304 | 4.21E-49 | -1.69E-46 |
| MS.gene051226 | MS.gene45281 | 0.807636 | 4.59E-50 | -1.69E-46 |
| MS.gene051226 | MS.gene46442 | 0.858202 | 9.69E-63 | -1.69E-46 |
| MS.gene051226 | MS.gene46918 | 0.858083 | 1.05E-62 | -1.69E-46 |
| MS.gene051226 | MS.gene49271 | 0.807052 | 6.10E-50 | -1.69E-46 |
| MS.gene051226 | MS.gene49627 | 0.810195 | 1.30E-50 | -1.69E-46 |
| MS.gene051226 | MS.gene49700 | 0.802557 | 5.30E-49 | -1.69E-46 |
| MS.gene051226 | MS.gene49733 | 0.800144 | 1.65E-48 | -1.69E-46 |
| MS.gene051226 | MS.gene50189 | 0.850761 | 1.38E-60 | -1.69E-46 |
| MS.gene051226 | MS.gene50190 | 0.805018 | 1.63E-49 | -1.69E-46 |
| MS.gene051226 | MS.gene54123 | 0.807602 | 4.66E-50 | -1.69E-46 |
| MS.gene051226 | MS.gene54187 | 0.818859 | 1.57E-52 | -1.69E-46 |
| MS.gene051226 | MS.gene54188 | 0.849 | 4.28E-60 | -1.69E-46 |
| MS.gene051226 | MS.gene54738 | 0.860375 | 2.16E-63 | -1.69E-46 |
| MS.gene051226 | MS.gene56623 | 0.826571 | 2.52E-54 | -1.69E-46 |
| MS.gene051226 | MS.gene56667 | 0.81675 | 4.71E-52 | -1.69E-46 |
| MS.gene051226 | MS.gene57009 | 0.862539 | 4.72E-64 | -1.69E-46 |
| MS.gene051226 | MS.gene57517 | 0.86021 | 2.42E-63 | -1.69E-46 |
| MS.gene051226 | MS.gene57519 | 0.823631 | 1.25E-53 | -1.69E-46 |
| MS.gene051226 | MS.gene57601 | 0.849786 | 2.59E-60 | -1.69E-46 |
| MS.gene051226 | MS.gene57704 | 0.865717 | 4.82E-65 | -1.69E-46 |
| MS.gene051226 | MS.gene57783 | 0.802681 | 5.00E-49 | -1.69E-46 |
| MS.gene051226 | MS.gene57980 | 0.806545 | 7.81E-50 | -1.69E-46 |
| MS.gene051226 | MS.gene58768 | 0.876896 | 9.69E-69 | -1.69E-46 |
| MS.gene051226 | MS.gene58769 | 0.872535 | 2.95E-67 | -1.69E-46 |
| MS.gene051226 | MS.gene58793 | 0.874203 | 8.11E-68 | -1.69E-46 |
| MS.gene051226 | MS.gene59435 | 0.829856 | 4.07E-55 | -1.69E-46 |
| MS.gene051226 | MS.gene61282 | 0.835593 | 1.53E-56 | -1.69E-46 |
| MS.gene051226 | MS.gene63043 | 0.810368 | 1.19E-50 | -1.69E-46 |
| MS.gene051226 | MS.gene65315 | 0.802174 | 6.36E-49 | -1.69E-46 |
| MS.gene051226 | MS.gene65380 | 0.802006 | 6.88E-49 | -1.69E-46 |
| MS.gene051226 | MS.gene65682 | 0.812539 | 4.03E-51 | -1.69E-46 |
| MS.gene051226 | MS.gene68028 | 0.822504 | 2.29E-53 | -1.69E-46 |
| MS.gene051226 | MS.gene70188 | 0.865248 | 6.78E-65 | -1.69E-46 |
| MS.gene051226 | MS.gene70277 | 0.819039 | 1.43E-52 | -1.69E-46 |
| MS.gene051226 | MS.gene71734 | 0.868247 | 7.53E-66 | -1.69E-46 |
| MS.gene051226 | MS.gene71994 | 0.813913 | 2.01E-51 | -1.69E-46 |
| MS.gene051226 | MS.gene72353 | 0.830141 | 3.47E-55 | -1.69E-46 |
| MS.gene051226 | MS.gene72611 | 0.843033 | 1.80E-58 | -1.69E-46 |
| MS.gene051226 | MS.gene73468 | 0.869242 | 3.58E-66 | -1.69E-46 |
| MS.gene051226 | MS.gene75798 | 0.808096 | 3.66E-50 | -1.69E-46 |
| MS.gene051226 | MS.gene77362 | 0.854199 | 1.44E-61 | -1.69E-46 |
| MS.gene051226 | MS.gene80201 | 0.81025 | 1.27E-50 | -1.69E-46 |
| MS.gene051226 | MS.gene81604 | 0.862869 | 3.73E-64 | -1.69E-46 |
| MS.gene051226 | MS.gene82042 | 0.815628 | 8.39E-52 | -1.69E-46 |
| MS.gene051226 | MS.gene83077 | 0.806115 | 9.62E-50 | -1.69E-46 |
| MS.gene051226 | MS.gene83604 | 0.85313 | 2.93E-61 | -1.69E-46 |
| MS.gene051226 | MS.gene83618 | 0.94187 | 1.96E-101 | -1.69E-46 |
| MS.gene051226 | MS.gene85281 | 0.824182 | 9.27E-54 | -1.69E-46 |
| MS.gene051226 | MS.gene88032 | 0.801019 | 1.10E-48 | -1.69E-46 |
| MS.gene051226 | MS.gene88946 | 0.80861 | 2.85E-50 | -1.69E-46 |
| MS.gene051226 | MS.gene90260 | 0.807378 | 5.20E-50 | -1.69E-46 |
| MS.gene051226 | MS.gene90263 | 0.805328 | 1.41E-49 | -1.69E-46 |
| MS.gene051226 | MS.gene90362 | 0.841231 | 5.39E-58 | -1.69E-46 |
| MS.gene051226 | MS.gene90467 | 0.844551 | 7.05E-59 | -1.69E-46 |
| MS.gene051226 | MS.gene90533 | 0.801657 | 8.12E-49 | -1.69E-46 |
| MS.gene051226 | MS.gene91539 | 0.851025 | 1.16E-60 | -1.69E-46 |
| MS.gene051226 | MS.gene92946 | 0.819613 | 1.06E-52 | -1.69E-46 |
| MS.gene051226 | MS.gene93972 | 0.845162 | 4.82E-59 | -1.69E-46 |
| MS.gene051226 | MS.gene95614 | 0.801542 | 8.57E-49 | -1.69E-46 |
| MS.gene051226 | MS.gene98294 | 0.852758 | 3.74E-61 | -1.69E-46 |
| MS.gene051226 | MS.gene98295 | 0.876329 | 1.52E-68 | -1.69E-46 |
| MS.gene051226 | MS.gene98721 | 0.825693 | 4.08E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051226.t1 | AT5G12010 | 59.302 | 430 | 149 | 4 | 1 | 417 | 86 | 502 | 0.0 | 531 |
| MS.gene051226.t1 | AT4G29780 | 59.620 | 421 | 164 | 4 | 1 | 417 | 122 | 540 | 1.48e-176 | 504 |
| MS.gene051226.t1 | AT3G55350 | 30.061 | 326 | 189 | 9 | 76 | 374 | 53 | 366 | 5.20e-43 | 155 |
| MS.gene051226.t1 | AT3G63270 | 29.618 | 314 | 188 | 10 | 76 | 363 | 43 | 349 | 1.16e-38 | 144 |
| MS.gene051226.t1 | AT3G19120 | 27.687 | 307 | 190 | 9 | 88 | 376 | 114 | 406 | 2.56e-25 | 107 |
| MS.gene051226.t1 | AT1G72275 | 26.797 | 306 | 162 | 14 | 88 | 374 | 92 | 354 | 4.99e-19 | 89.0 |
| MS.gene051226.t1 | AT1G72275 | 26.797 | 306 | 162 | 14 | 88 | 374 | 92 | 354 | 5.95e-19 | 88.6 |
Find 100 sgRNAs with CRISPR-Local
Find 108 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATTCTTCAAATTTCCTAATT+TGG | 0.108987 | 2.3:+49270511 | None:intergenic |
| TAGGAAATTTGAAGAATATT+TGG | 0.147062 | 2.3:-49270505 | MS.gene051226:CDS |
| CAAGATTTGCCTGTTGTTCT+TGG | 0.174992 | 2.3:-49270296 | MS.gene051226:CDS |
| AAATTCAGGTTATTCACTTA+TGG | 0.234627 | 2.3:-49270474 | MS.gene051226:CDS |
| ATTAGTGAAATTGAGAAAAT+TGG | 0.245237 | 2.3:-49270386 | MS.gene051226:CDS |
| ATTTGGATAGTTGGAAATTC+AGG | 0.257969 | 2.3:-49270488 | MS.gene051226:CDS |
| AATGCAAGATAATGCAGTTT+TGG | 0.265766 | 2.3:-49270558 | MS.gene051226:CDS |
| GTCAGATTCTTATGCTTATA+TGG | 0.273058 | 2.3:+49270437 | None:intergenic |
| TCCTTCATTGTTTCTTCATC+TGG | 0.276668 | 2.3:+49270800 | None:intergenic |
| TTGAAGAATATTTGGATAGT+TGG | 0.287680 | 2.3:-49270497 | MS.gene051226:CDS |
| TCAGCTATTGTATCTGGTTC+TGG | 0.289548 | 2.3:-49271235 | MS.gene051226:CDS |
| TTCTTCCATGTAATGATTCA+TGG | 0.295521 | 2.3:+49271339 | None:intergenic |
| GCTGCTTCAGCTATTGTATC+TGG | 0.300058 | 2.3:-49271241 | MS.gene051226:CDS |
| TGAAGATGGTGGTTGTTCTT+TGG | 0.311168 | 2.3:-49270180 | MS.gene051226:CDS |
| TTATATGAGAGGGCCAAATT+AGG | 0.319401 | 2.3:-49270524 | MS.gene051226:CDS |
| AGGCTGGTTTCTAAGAAATT+CGG | 0.322875 | 2.3:-49270908 | MS.gene051226:CDS |
| TTTACCTTAGGTGCAATTAT+AGG | 0.323232 | 2.3:+49270707 | None:intergenic |
| GGCTGGTTTCTAAGAAATTC+GGG | 0.333472 | 2.3:-49270907 | MS.gene051226:CDS |
| CAACAAGCACCAAGAACAAC+AGG | 0.334974 | 2.3:+49270287 | None:intergenic |
| ACCGCCACACGCTGGCGAAC+CGG | 0.336332 | 2.3:+49270965 | None:intergenic |
| TTGTTCTTTGGATTCTTTGA+AGG | 0.339140 | 2.3:-49270168 | MS.gene051226:CDS |
| CAAGAGTTTGAGGGTAGTTT+CGG | 0.352557 | 2.3:-49270770 | MS.gene051226:CDS |
| TACATTACACCACCAACATT+TGG | 0.366525 | 2.3:+49270743 | None:intergenic |
| AAAATTGGTAAAGATGCTTT+TGG | 0.369188 | 2.3:-49270371 | MS.gene051226:CDS |
| AGGTTATTCACTTATGGATT+GGG | 0.375307 | 2.3:-49270468 | MS.gene051226:CDS |
| CAGGTTATTCACTTATGGAT+TGG | 0.377146 | 2.3:-49270469 | MS.gene051226:CDS |
| CATCATGGTCGTGCTGGTAC+TGG | 0.387672 | 2.3:-49270116 | MS.gene051226:CDS |
| GACGCTGCGTGACGCTATTC+CGG | 0.388589 | 2.3:-49270984 | MS.gene051226:CDS |
| TGGCGACTGGCGAACCGCTT+AGG | 0.409248 | 2.3:-49270928 | MS.gene051226:CDS |
| ACCCACAGCCTCCGTTGAAT+AGG | 0.413538 | 2.3:+49271151 | None:intergenic |
| TTGCAACCGTGAAGATTTCC+CGG | 0.413793 | 2.3:-49271098 | MS.gene051226:CDS |
| CAACTCTCCCACCAGTCTTT+AGG | 0.414933 | 2.3:+49271118 | None:intergenic |
| TGGTAGGTTGAAAGGTAGGT+GGG | 0.415263 | 2.3:-49270351 | MS.gene051226:CDS |
| TGAGATTATGAATGAAGAAA+TGG | 0.419917 | 2.3:-49270246 | MS.gene051226:CDS |
| AGCTATTCAGTAACTGTTCA+AGG | 0.427841 | 2.3:-49270635 | MS.gene051226:CDS |
| ATTTCCCGGATGATGAATTC+CGG | 0.433406 | 2.3:-49271084 | MS.gene051226:CDS |
| GTTTCTAAGAAATTCGGGTT+AGG | 0.437170 | 2.3:-49270902 | MS.gene051226:CDS |
| TGGTCCAGACCAACGTGGTC+TGG | 0.437477 | 2.3:-49271215 | MS.gene051226:CDS |
| TAAGCGGTTCGCCAGTCGCC+AGG | 0.444621 | 2.3:+49270930 | None:intergenic |
| GCTCATTATTTGTTACATCA+TGG | 0.448680 | 2.3:-49270131 | MS.gene051226:CDS |
| AGCCTATTCAACGGAGGCTG+TGG | 0.449951 | 2.3:-49271153 | MS.gene051226:CDS |
| GACTGGCGAACCGCTTAGGC+TGG | 0.458846 | 2.3:-49270924 | MS.gene051226:CDS |
| TGGTCCAGACCACGTTGGTC+TGG | 0.459547 | 2.3:+49271211 | None:intergenic |
| TAATGCAGAAATTTCTTCGT+TGG | 0.465479 | 2.3:-49270823 | MS.gene051226:CDS |
| TTGGTAGGTTGAAAGGTAGG+TGG | 0.468359 | 2.3:-49270352 | MS.gene051226:CDS |
| CATCGCCGGAATTCATCATC+CGG | 0.483016 | 2.3:+49271079 | None:intergenic |
| TTCGGTATTCCAAATGTTGG+TGG | 0.485519 | 2.3:-49270752 | MS.gene051226:CDS |
| GGAGGCTGTGGGTGAAAGAC+CGG | 0.491651 | 2.3:-49271141 | MS.gene051226:CDS |
| AATATCTTTCGAATCTGTAC+CGG | 0.495460 | 2.3:+49271186 | None:intergenic |
| TGTATATACCGCCTGGCGAC+TGG | 0.495948 | 2.3:-49270941 | MS.gene051226:CDS |
| TTGTTACATCATGGTCGTGC+TGG | 0.496299 | 2.3:-49270122 | MS.gene051226:CDS |
| TTACTGATGTGTGTATTGGT+TGG | 0.498611 | 2.3:-49270589 | MS.gene051226:CDS |
| TACAAACCAACATTTACCTT+AGG | 0.499068 | 2.3:+49270695 | None:intergenic |
| GATGTGTGTATTGGTTGGCC+TGG | 0.500931 | 2.3:-49270584 | MS.gene051226:CDS |
| CATCATCCGGGAAATCTTCA+CGG | 0.507925 | 2.3:+49271092 | None:intergenic |
| CAAGGTGTGGTAAATAGTAA+AGG | 0.508102 | 2.3:-49270617 | MS.gene051226:CDS |
| TGAAAGACCGGCCTAAAGAC+TGG | 0.509719 | 2.3:-49271129 | MS.gene051226:CDS |
| GGTAGGTTGAAAGGTAGGTG+GGG | 0.512384 | 2.3:-49270350 | MS.gene051226:CDS |
| GTAGGTTGAAAGGTAGGTGG+GGG | 0.513890 | 2.3:-49270349 | MS.gene051226:CDS |
| ATGAAGAAATGGATGATGAA+TGG | 0.517962 | 2.3:-49270235 | MS.gene051226:CDS |
| CTGCAACGAGCTGGATTCAT+CGG | 0.520703 | 2.3:-49271023 | MS.gene051226:CDS |
| TGTTCCTATAATTGCACCTA+AGG | 0.522840 | 2.3:-49270711 | MS.gene051226:CDS |
| ATTGCACCTAAGGTAAATGT+TGG | 0.524057 | 2.3:-49270701 | MS.gene051226:CDS |
| CAACATGATCTGCAACGAGC+TGG | 0.528812 | 2.3:-49271032 | MS.gene051226:CDS |
| GCCTCCGTTGAATAGGCTGC+TGG | 0.530009 | 2.3:+49271158 | None:intergenic |
| AGTTTCGGTATTCCAAATGT+TGG | 0.530144 | 2.3:-49270755 | MS.gene051226:CDS |
| GGAAACGAAACAAGAGTTTG+AGG | 0.531217 | 2.3:-49270780 | MS.gene051226:CDS |
| TCGTTGTTGTTTGATGATGT+TGG | 0.531698 | 2.3:+49271290 | None:intergenic |
| TCCGGTTCGCCAGCGTGTGG+CGG | 0.537854 | 2.3:-49270966 | MS.gene051226:CDS |
| GGATGATGAATGGAAGTTTG+AGG | 0.540969 | 2.3:-49270225 | MS.gene051226:CDS |
| ATATCTTTCGAATCTGTACC+GGG | 0.545779 | 2.3:+49271187 | None:intergenic |
| CTTTCGAATCTGTACCGGGC+TGG | 0.548784 | 2.3:+49271191 | None:intergenic |
| GCCAGATGAAGAAACAATGA+AGG | 0.551617 | 2.3:-49270801 | MS.gene051226:CDS |
| AATCTGACGTGGACACAACA+TGG | 0.554899 | 2.3:-49270422 | MS.gene051226:CDS |
| GCATTATCTTGCATTGAACC+AGG | 0.558688 | 2.3:+49270566 | None:intergenic |
| GAAACGAAACAAGAGTTTGA+GGG | 0.571318 | 2.3:-49270779 | MS.gene051226:CDS |
| GCCTATTCAACGGAGGCTGT+GGG | 0.575143 | 2.3:-49271152 | MS.gene051226:CDS |
| GGCGGTATGTATATACCGCC+TGG | 0.576391 | 2.3:-49270948 | MS.gene051226:CDS |
| ATCGCCGGAATTCATCATCC+GGG | 0.580423 | 2.3:+49271080 | None:intergenic |
| TACTCATCCGAAAACATCGC+CGG | 0.582531 | 2.3:+49271065 | None:intergenic |
| TTCTTAGAAACCAGCCTAAG+CGG | 0.597242 | 2.3:+49270914 | None:intergenic |
| TTCAGTAACTGTTCAAGGTG+TGG | 0.599865 | 2.3:-49270630 | MS.gene051226:CDS |
| GATGAAATGATTGTTGAAGA+TGG | 0.603950 | 2.3:-49270194 | MS.gene051226:CDS |
| ACAAACCATGAATCATTACA+TGG | 0.604340 | 2.3:-49271344 | None:intergenic |
| CAACGTGGTCTGGACCAGCC+CGG | 0.612903 | 2.3:-49271205 | MS.gene051226:CDS |
| AGACCGGCCTAAAGACTGGT+GGG | 0.625890 | 2.3:-49271125 | MS.gene051226:CDS |
| TCTCCCACCAGTCTTTAGGC+CGG | 0.626133 | 2.3:+49271122 | None:intergenic |
| GTTGGTCTAACTGAGAGTAA+TGG | 0.636217 | 2.3:+49271308 | None:intergenic |
| TATTCCGGTTCGCCAGCGTG+TGG | 0.639543 | 2.3:-49270969 | MS.gene051226:CDS |
| ATCTGACGTGGACACAACAT+GGG | 0.652309 | 2.3:-49270421 | MS.gene051226:CDS |
| TCAACCAGCAGCCTATTCAA+CGG | 0.665567 | 2.3:-49271162 | MS.gene051226:CDS |
| AAGACCGGCCTAAAGACTGG+TGG | 0.667437 | 2.3:-49271126 | MS.gene051226:CDS |
| TAACACAAGCTTATGACACG+TGG | 0.669447 | 2.3:+49270874 | None:intergenic |
| GAAATGATTGTTGAAGATGG+TGG | 0.682776 | 2.3:-49270191 | MS.gene051226:CDS |
| GCGGTTCGCCAGTCGCCAGG+CGG | 0.683772 | 2.3:+49270933 | None:intergenic |
| ACCAGCAGCCTATTCAACGG+AGG | 0.702601 | 2.3:-49271159 | MS.gene051226:CDS |
| CGGGCTGGTCCAGACCACGT+TGG | 0.734063 | 2.3:+49271206 | None:intergenic |
| GGTTCTGGTCCAGACCAACG+TGG | 0.734063 | 2.3:-49271220 | MS.gene051226:CDS |
| ATATACATACCGCCACACGC+TGG | 0.742881 | 2.3:+49270957 | None:intergenic |
| ATAAGCATAAGAATCTGACG+TGG | 0.747957 | 2.3:-49270433 | MS.gene051226:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTAGTGAAATTGAGAAAAT+TGG | - | chr2.3:49271057-49271076 | MS.gene051226:CDS | 20.0% |
| !! | ATTCTTCAAATTTCCTAATT+TGG | + | chr2.3:49270935-49270954 | None:intergenic | 20.0% |
| !! | TAGGAAATTTGAAGAATATT+TGG | - | chr2.3:49270938-49270957 | MS.gene051226:CDS | 20.0% |
| ! | TGAGATTATGAATGAAGAAA+TGG | - | chr2.3:49271197-49271216 | MS.gene051226:CDS | 25.0% |
| ! | TTGAAGAATATTTGGATAGT+TGG | - | chr2.3:49270946-49270965 | MS.gene051226:CDS | 25.0% |
| !! | AAAATTGGTAAAGATGCTTT+TGG | - | chr2.3:49271072-49271091 | MS.gene051226:CDS | 25.0% |
| !! | AAATTCAGGTTATTCACTTA+TGG | - | chr2.3:49270969-49270988 | MS.gene051226:CDS | 25.0% |
| !!! | AAAAAAGTGCTTTATATGAG+AGG | - | chr2.3:49270908-49270927 | MS.gene051226:CDS | 25.0% |
| !!! | AAAAAGTGCTTTATATGAGA+GGG | - | chr2.3:49270909-49270928 | MS.gene051226:CDS | 25.0% |
| !!! | TTACTGAATAGCTAGTTTTT+TGG | + | chr2.3:49270801-49270820 | None:intergenic | 25.0% |
| AATATCTTTCGAATCTGTAC+CGG | + | chr2.3:49270260-49270279 | None:intergenic | 30.0% | |
| AGGTTATTCACTTATGGATT+GGG | - | chr2.3:49270975-49270994 | MS.gene051226:CDS | 30.0% | |
| ATGAAGAAATGGATGATGAA+TGG | - | chr2.3:49271208-49271227 | MS.gene051226:CDS | 30.0% | |
| ATTTGGATAGTTGGAAATTC+AGG | - | chr2.3:49270955-49270974 | MS.gene051226:CDS | 30.0% | |
| GATGAAATGATTGTTGAAGA+TGG | - | chr2.3:49271249-49271268 | MS.gene051226:CDS | 30.0% | |
| GTCAGATTCTTATGCTTATA+TGG | + | chr2.3:49271009-49271028 | None:intergenic | 30.0% | |
| TAATGCAGAAATTTCTTCGT+TGG | - | chr2.3:49270620-49270639 | MS.gene051226:CDS | 30.0% | |
| TACAAACCAACATTTACCTT+AGG | + | chr2.3:49270751-49270770 | None:intergenic | 30.0% | |
| TTAACAAAAAACTCACTGAG+AGG | - | chr2.3:49270773-49270792 | MS.gene051226:CDS | 30.0% | |
| TTGTTCTTTGGATTCTTTGA+AGG | - | chr2.3:49271275-49271294 | MS.gene051226:CDS | 30.0% | |
| TTTACCTTAGGTGCAATTAT+AGG | + | chr2.3:49270739-49270758 | None:intergenic | 30.0% | |
| ! | AATGCAAGATAATGCAGTTT+TGG | - | chr2.3:49270885-49270904 | MS.gene051226:CDS | 30.0% |
| ! | GCTCATTATTTGTTACATCA+TGG | - | chr2.3:49271312-49271331 | MS.gene051226:CDS | 30.0% |
| ! | GTTTTTACTGATGTGTGTAT+TGG | - | chr2.3:49270850-49270869 | MS.gene051226:CDS | 30.0% |
| AGCTATTCAGTAACTGTTCA+AGG | - | chr2.3:49270808-49270827 | MS.gene051226:CDS | 35.0% | |
| AGTTTCGGTATTCCAAATGT+TGG | - | chr2.3:49270688-49270707 | MS.gene051226:CDS | 35.0% | |
| ATAAGCATAAGAATCTGACG+TGG | - | chr2.3:49271010-49271029 | MS.gene051226:CDS | 35.0% | |
| ATATCTTTCGAATCTGTACC+GGG | + | chr2.3:49270259-49270278 | None:intergenic | 35.0% | |
| ATTGCACCTAAGGTAAATGT+TGG | - | chr2.3:49270742-49270761 | MS.gene051226:CDS | 35.0% | |
| CAAGGTGTGGTAAATAGTAA+AGG | - | chr2.3:49270826-49270845 | MS.gene051226:CDS | 35.0% | |
| CAGGTTATTCACTTATGGAT+TGG | - | chr2.3:49270974-49270993 | MS.gene051226:CDS | 35.0% | |
| GAAACGAAACAAGAGTTTGA+GGG | - | chr2.3:49270664-49270683 | MS.gene051226:CDS | 35.0% | |
| GAAATGATTGTTGAAGATGG+TGG | - | chr2.3:49271252-49271271 | MS.gene051226:CDS | 35.0% | |
| TACATTACACCACCAACATT+TGG | + | chr2.3:49270703-49270722 | None:intergenic | 35.0% | |
| TGTTCCTATAATTGCACCTA+AGG | - | chr2.3:49270732-49270751 | MS.gene051226:CDS | 35.0% | |
| TTATATGAGAGGGCCAAATT+AGG | - | chr2.3:49270919-49270938 | MS.gene051226:CDS | 35.0% | |
| ! | GTTTCTAAGAAATTCGGGTT+AGG | - | chr2.3:49270541-49270560 | MS.gene051226:CDS | 35.0% |
| ! | TCCTTCATTGTTTCTTCATC+TGG | + | chr2.3:49270646-49270665 | None:intergenic | 35.0% |
| ! | TTACTGATGTGTGTATTGGT+TGG | - | chr2.3:49270854-49270873 | MS.gene051226:CDS | 35.0% |
| ! | TTGGTAAAGATGCTTTTGGT+AGG | - | chr2.3:49271076-49271095 | MS.gene051226:CDS | 35.0% |
| !! | AGGCTGGTTTCTAAGAAATT+CGG | - | chr2.3:49270535-49270554 | MS.gene051226:CDS | 35.0% |
| !! | TCGTTGTTGTTTGATGATGT+TGG | + | chr2.3:49270156-49270175 | None:intergenic | 35.0% |
| ATTTCCCGGATGATGAATTC+CGG | - | chr2.3:49270359-49270378 | MS.gene051226:CDS | 40.0% | |
| CAAGAGTTTGAGGGTAGTTT+CGG | - | chr2.3:49270673-49270692 | MS.gene051226:CDS | 40.0% | |
| CAAGATTTGCCTGTTGTTCT+TGG | - | chr2.3:49271147-49271166 | MS.gene051226:CDS | 40.0% | |
| GCATTATCTTGCATTGAACC+AGG | + | chr2.3:49270880-49270899 | None:intergenic | 40.0% | |
| GCCAGATGAAGAAACAATGA+AGG | - | chr2.3:49270642-49270661 | MS.gene051226:CDS | 40.0% | |
| GGAAACGAAACAAGAGTTTG+AGG | - | chr2.3:49270663-49270682 | MS.gene051226:CDS | 40.0% | |
| GGATGATGAATGGAAGTTTG+AGG | - | chr2.3:49271218-49271237 | MS.gene051226:CDS | 40.0% | |
| TAACACAAGCTTATGACACG+TGG | + | chr2.3:49270572-49270591 | None:intergenic | 40.0% | |
| TTCAGTAACTGTTCAAGGTG+TGG | - | chr2.3:49270813-49270832 | MS.gene051226:CDS | 40.0% | |
| TTCGGTATTCCAAATGTTGG+TGG | - | chr2.3:49270691-49270710 | MS.gene051226:CDS | 40.0% | |
| TTCTTAGAAACCAGCCTAAG+CGG | + | chr2.3:49270532-49270551 | None:intergenic | 40.0% | |
| ! | ATGAATTCCGGCGATGTTTT+CGG | - | chr2.3:49270371-49270390 | MS.gene051226:CDS | 40.0% |
| ! | GTTGGTCTAACTGAGAGTAA+TGG | + | chr2.3:49270138-49270157 | None:intergenic | 40.0% |
| ! | TCAGCTATTGTATCTGGTTC+TGG | - | chr2.3:49270208-49270227 | MS.gene051226:CDS | 40.0% |
| ! | TGAAGATGGTGGTTGTTCTT+TGG | - | chr2.3:49271263-49271282 | MS.gene051226:CDS | 40.0% |
| !! | CTTTTGGTAGGTTGAAAGGT+AGG | - | chr2.3:49271088-49271107 | MS.gene051226:CDS | 40.0% |
| !! | GATGCTTTTGGTAGGTTGAA+AGG | - | chr2.3:49271084-49271103 | MS.gene051226:CDS | 40.0% |
| !! | GGCTGGTTTCTAAGAAATTC+GGG | - | chr2.3:49270536-49270555 | MS.gene051226:CDS | 40.0% |
| AATCTGACGTGGACACAACA+TGG | - | chr2.3:49271021-49271040 | MS.gene051226:CDS | 45.0% | |
| ATCTGACGTGGACACAACAT+GGG | - | chr2.3:49271022-49271041 | MS.gene051226:CDS | 45.0% | |
| CAACAAGCACCAAGAACAAC+AGG | + | chr2.3:49271159-49271178 | None:intergenic | 45.0% | |
| CATCATCCGGGAAATCTTCA+CGG | + | chr2.3:49270354-49270373 | None:intergenic | 45.0% | |
| GCTGCTTCAGCTATTGTATC+TGG | - | chr2.3:49270202-49270221 | MS.gene051226:CDS | 45.0% | |
| TACTCATCCGAAAACATCGC+CGG | + | chr2.3:49270381-49270400 | None:intergenic | 45.0% | |
| TCAACCAGCAGCCTATTCAA+CGG | - | chr2.3:49270281-49270300 | MS.gene051226:CDS | 45.0% | |
| TTGCAACCGTGAAGATTTCC+CGG | - | chr2.3:49270345-49270364 | MS.gene051226:CDS | 45.0% | |
| TTGTTACATCATGGTCGTGC+TGG | - | chr2.3:49271321-49271340 | MS.gene051226:CDS | 45.0% | |
| ! | TGGTAGGTTGAAAGGTAGGT+GGG | - | chr2.3:49271092-49271111 | MS.gene051226:CDS | 45.0% |
| ! | TTGGTAGGTTGAAAGGTAGG+TGG | - | chr2.3:49271091-49271110 | MS.gene051226:CDS | 45.0% |
| ATATACATACCGCCACACGC+TGG | + | chr2.3:49270489-49270508 | None:intergenic | 50.0% | |
| ATCGCCGGAATTCATCATCC+GGG | + | chr2.3:49270366-49270385 | None:intergenic | 50.0% | |
| CAACATGATCTGCAACGAGC+TGG | - | chr2.3:49270411-49270430 | MS.gene051226:CDS | 50.0% | |
| CAACTCTCCCACCAGTCTTT+AGG | + | chr2.3:49270328-49270347 | None:intergenic | 50.0% | |
| CATCGCCGGAATTCATCATC+CGG | + | chr2.3:49270367-49270386 | None:intergenic | 50.0% | |
| CTGCAACGAGCTGGATTCAT+CGG | - | chr2.3:49270420-49270439 | MS.gene051226:CDS | 50.0% | |
| TGAAAGACCGGCCTAAAGAC+TGG | - | chr2.3:49270314-49270333 | MS.gene051226:CDS | 50.0% | |
| ! | GGTAGGTTGAAAGGTAGGTG+GGG | - | chr2.3:49271093-49271112 | MS.gene051226:CDS | 50.0% |
| ! | GTAGGTTGAAAGGTAGGTGG+GGG | - | chr2.3:49271094-49271113 | MS.gene051226:CDS | 50.0% |
| !! | GATGTGTGTATTGGTTGGCC+TGG | - | chr2.3:49270859-49270878 | MS.gene051226:CDS | 50.0% |
| !! | GGTGGGGGTGTTTGAAGAAA+AGG | - | chr2.3:49271109-49271128 | MS.gene051226:CDS | 50.0% |
| AAGACCGGCCTAAAGACTGG+TGG | - | chr2.3:49270317-49270336 | MS.gene051226:CDS | 55.0% | |
| ACCAGCAGCCTATTCAACGG+AGG | - | chr2.3:49270284-49270303 | MS.gene051226:CDS | 55.0% | |
| ACCCACAGCCTCCGTTGAAT+AGG | + | chr2.3:49270295-49270314 | None:intergenic | 55.0% | |
| AGACCGGCCTAAAGACTGGT+GGG | - | chr2.3:49270318-49270337 | MS.gene051226:CDS | 55.0% | |
| AGCCTATTCAACGGAGGCTG+TGG | - | chr2.3:49270290-49270309 | MS.gene051226:CDS | 55.0% | |
| CTTTCGAATCTGTACCGGGC+TGG | + | chr2.3:49270255-49270274 | None:intergenic | 55.0% | |
| GCCTATTCAACGGAGGCTGT+GGG | - | chr2.3:49270291-49270310 | MS.gene051226:CDS | 55.0% | |
| GGCGGTATGTATATACCGCC+TGG | - | chr2.3:49270495-49270514 | MS.gene051226:CDS | 55.0% | |
| TCTCCCACCAGTCTTTAGGC+CGG | + | chr2.3:49270324-49270343 | None:intergenic | 55.0% | |
| TGTATATACCGCCTGGCGAC+TGG | - | chr2.3:49270502-49270521 | MS.gene051226:CDS | 55.0% | |
| ! | CATCATGGTCGTGCTGGTAC+TGG | - | chr2.3:49271327-49271346 | MS.gene051226:CDS | 55.0% |
| GACGCTGCGTGACGCTATTC+CGG | - | chr2.3:49270459-49270478 | MS.gene051226:CDS | 60.0% | |
| GCCTCCGTTGAATAGGCTGC+TGG | + | chr2.3:49270288-49270307 | None:intergenic | 60.0% | |
| GGAGGCTGTGGGTGAAAGAC+CGG | - | chr2.3:49270302-49270321 | MS.gene051226:CDS | 60.0% | |
| TGGTCCAGACCAACGTGGTC+TGG | - | chr2.3:49270228-49270247 | MS.gene051226:CDS | 60.0% | |
| TGGTCCAGACCACGTTGGTC+TGG | + | chr2.3:49270235-49270254 | None:intergenic | 60.0% | |
| ! | GGTTCTGGTCCAGACCAACG+TGG | - | chr2.3:49270223-49270242 | MS.gene051226:CDS | 60.0% |
| ! | TATTCCGGTTCGCCAGCGTG+TGG | - | chr2.3:49270474-49270493 | MS.gene051226:CDS | 60.0% |
| CAACGTGGTCTGGACCAGCC+CGG | - | chr2.3:49270238-49270257 | MS.gene051226:CDS | 65.0% | |
| GACTGGCGAACCGCTTAGGC+TGG | - | chr2.3:49270519-49270538 | MS.gene051226:CDS | 65.0% | |
| TAAGCGGTTCGCCAGTCGCC+AGG | + | chr2.3:49270516-49270535 | None:intergenic | 65.0% | |
| TGGCGACTGGCGAACCGCTT+AGG | - | chr2.3:49270515-49270534 | MS.gene051226:CDS | 65.0% | |
| ACCGCCACACGCTGGCGAAC+CGG | + | chr2.3:49270481-49270500 | None:intergenic | 70.0% | |
| ! | TCCGGTTCGCCAGCGTGTGG+CGG | - | chr2.3:49270477-49270496 | MS.gene051226:CDS | 70.0% |
| !! | CGGGCTGGTCCAGACCACGT+TGG | + | chr2.3:49270240-49270259 | None:intergenic | 70.0% |
| GCGGTTCGCCAGTCGCCAGG+CGG | + | chr2.3:49270513-49270532 | None:intergenic | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.3 | gene | 49270106 | 49271359 | 49270106 | ID=MS.gene051226 |
| chr2.3 | mRNA | 49270106 | 49271359 | 49270106 | ID=MS.gene051226.t1;Parent=MS.gene051226 |
| chr2.3 | exon | 49270106 | 49271359 | 49270106 | ID=MS.gene051226.t1.exon1;Parent=MS.gene051226.t1 |
| chr2.3 | CDS | 49270106 | 49271359 | 49270106 | ID=cds.MS.gene051226.t1;Parent=MS.gene051226.t1 |
| Gene Sequence |
| Protein sequence |
