AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene072635

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene072635.t1 MTR_7g110810 80.106 377 27 6 1 333 1 373 0.0 557
MS.gene072635.t1 MTR_1g084980 50.323 155 62 4 112 262 409 552 5.19e-35 135
MS.gene072635.t1 MTR_1g069155 39.412 170 90 3 78 243 232 392 7.69e-28 114
MS.gene072635.t1 MTR_7g111320 40.654 214 93 8 112 303 379 580 8.18e-28 114
MS.gene072635.t1 MTR_7g099540 51.240 121 48 4 128 248 223 332 1.89e-25 106
MS.gene072635.t1 MTR_3g449770 40.559 143 75 4 128 269 278 411 4.81e-24 102
MS.gene072635.t1 MTR_7g099540 48.819 127 48 5 128 248 223 338 3.40e-23 100
MS.gene072635.t1 MTR_1g019240 55.294 85 36 2 141 225 65 147 3.49e-20 89.0
MS.gene072635.t1 MTR_5g017040 42.276 123 64 2 119 238 62 180 1.59e-19 88.2
MS.gene072635.t1 MTR_4g131160 55.357 56 25 0 174 229 207 262 8.42e-16 78.2
MS.gene072635.t1 MTR_4g131160 55.357 56 25 0 174 229 210 265 8.54e-16 78.2
MS.gene072635.t1 MTR_1g084980 41.176 119 61 3 112 226 409 522 1.26e-15 78.2
MS.gene072635.t1 MTR_1g080890 51.786 56 27 0 174 229 204 259 5.93e-15 75.1
MS.gene072635.t1 MTR_3g103030 36.735 98 53 2 160 255 224 314 4.17e-13 69.3
MS.gene072635.t1 MTR_1g017350 46.970 66 34 1 174 238 271 336 8.43e-13 69.3
MS.gene072635.t1 MTR_1g017350 46.970 66 34 1 174 238 271 336 8.98e-13 68.9
MS.gene072635.t1 MTR_1g017350 46.970 66 34 1 174 238 271 336 9.37e-13 68.9
MS.gene072635.t1 MTR_1g017350 46.970 66 34 1 174 238 271 336 1.01e-12 68.9
MS.gene072635.t1 MTR_1g106460 35.065 77 43 1 179 255 254 323 3.35e-12 67.0
MS.gene072635.t1 MTR_3g116770 36.111 108 60 3 174 272 202 309 1.26e-11 65.5
MS.gene072635.t1 MTR_3g116770 36.111 108 60 3 174 272 202 309 1.30e-11 65.5
MS.gene072635.t1 MTR_5g048860 35.652 115 59 3 174 273 376 490 1.62e-11 65.5
MS.gene072635.t1 MTR_4g070320 35.514 107 63 3 129 234 114 215 2.49e-11 64.3
MS.gene072635.t1 MTR_8g024770 29.365 126 79 1 104 229 220 335 2.59e-11 64.7
MS.gene072635.t1 MTR_0246s0020 45.588 68 37 0 158 225 183 250 2.71e-11 64.3
MS.gene072635.t1 MTR_0246s0020 45.588 68 37 0 158 225 183 250 2.83e-11 64.3
MS.gene072635.t1 MTR_8g065740 44.118 68 37 1 174 240 316 383 3.91e-11 64.3
MS.gene072635.t1 MTR_5g005110 33.333 81 47 1 174 254 235 308 5.45e-11 63.2
MS.gene072635.t1 MTR_4g009110 47.297 74 39 0 146 219 147 220 6.81e-11 63.2
MS.gene072635.t1 MTR_7g053410 46.552 58 30 1 174 230 328 385 7.99e-11 63.2
MS.gene072635.t1 MTR_7g053410 46.552 58 30 1 174 230 328 385 9.18e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene072635.t1 AT2G46970 39.216 204 109 8 112 309 139 333 4.68e-31 120
MS.gene072635.t1 AT2G46970 39.216 204 109 8 112 309 148 342 6.85e-31 120
MS.gene072635.t1 AT2G46970 39.216 204 109 8 112 309 170 364 7.27e-31 120
MS.gene072635.t1 AT1G09530 47.368 133 61 3 110 234 277 408 2.23e-29 118
MS.gene072635.t1 AT1G09530 47.368 133 61 3 110 234 277 408 2.23e-29 118
MS.gene072635.t1 AT1G09530 47.368 133 61 3 110 234 277 408 2.23e-29 118
MS.gene072635.t1 AT1G09530 47.368 133 61 3 110 234 277 408 2.23e-29 118
MS.gene072635.t1 AT1G09530 47.368 133 61 3 110 234 277 408 2.23e-29 118
MS.gene072635.t1 AT1G09530 47.368 133 61 3 110 234 277 408 2.23e-29 118
MS.gene072635.t1 AT2G20180 40.964 166 83 3 146 307 190 344 1.38e-27 112
MS.gene072635.t1 AT2G20180 40.964 166 83 3 146 307 190 344 1.38e-27 112
MS.gene072635.t1 AT2G20180 40.964 166 83 3 146 307 190 344 1.38e-27 112
MS.gene072635.t1 AT2G20180 40.964 166 83 3 146 307 261 415 6.20e-27 110
MS.gene072635.t1 AT2G20180 40.964 166 83 3 146 307 261 415 6.20e-27 110
MS.gene072635.t1 AT2G20180 40.964 166 83 3 146 307 261 415 6.20e-27 110
MS.gene072635.t1 AT2G20180 40.964 166 83 3 146 307 261 415 6.20e-27 110
MS.gene072635.t1 AT4G28800 37.297 185 90 8 98 277 198 361 4.17e-23 99.8
MS.gene072635.t1 AT2G20180 54.430 79 36 0 146 224 261 339 3.90e-22 95.9
MS.gene072635.t1 AT3G59060 36.620 213 119 8 128 328 215 423 1.61e-21 95.1
MS.gene072635.t1 AT5G67110 45.045 111 57 3 154 261 79 188 1.66e-21 91.3
MS.gene072635.t1 AT4G28790 39.041 146 71 5 140 277 245 380 2.49e-21 94.4
MS.gene072635.t1 AT3G59060 38.503 187 104 6 128 304 215 400 3.90e-21 94.0
MS.gene072635.t1 AT3G59060 38.503 187 104 6 128 304 215 400 3.90e-21 94.0
MS.gene072635.t1 AT3G59060 38.503 187 104 6 128 304 215 400 3.90e-21 94.0
MS.gene072635.t1 AT4G36930 57.746 71 25 1 169 234 197 267 1.20e-19 89.0
MS.gene072635.t1 AT4G28800 36.464 181 91 7 98 277 210 367 1.53e-19 89.4
MS.gene072635.t1 AT2G43010 52.041 98 37 2 128 225 226 313 7.66e-18 84.3
MS.gene072635.t1 AT2G43010 52.041 98 37 2 128 225 226 313 7.73e-18 84.3
MS.gene072635.t1 AT2G43010 52.041 98 37 2 128 225 233 320 8.60e-18 84.3
MS.gene072635.t1 AT2G43010 52.041 98 37 2 128 225 226 313 9.78e-18 84.0
MS.gene072635.t1 AT2G43010 52.041 98 37 2 128 225 268 355 1.19e-17 84.0
MS.gene072635.t1 AT4G28815 42.857 91 35 2 161 251 66 139 1.59e-17 80.9
MS.gene072635.t1 AT3G62090 36.842 171 99 6 157 325 159 322 2.04e-17 82.4
MS.gene072635.t1 AT3G62090 36.842 171 99 6 157 325 176 339 2.24e-17 82.4
MS.gene072635.t1 AT2G24260 57.143 56 24 0 174 229 46 101 4.69e-17 79.7
MS.gene072635.t1 AT4G28815 42.857 91 35 2 161 251 142 215 4.86e-17 80.9
MS.gene072635.t1 AT4G00050 56.000 75 30 2 154 226 95 168 7.24e-17 80.1
MS.gene072635.t1 AT4G00050 56.000 75 30 2 154 226 197 270 1.24e-16 80.5
MS.gene072635.t1 AT4G00050 56.000 75 30 2 154 226 197 270 1.33e-16 80.5
MS.gene072635.t1 AT4G28790 46.591 88 42 2 140 223 245 331 1.44e-16 79.7
MS.gene072635.t1 AT2G24260 57.143 56 24 0 174 229 149 204 2.21e-16 79.3
MS.gene072635.t1 AT4G30980 55.357 56 25 0 174 229 141 196 5.99e-16 77.8
MS.gene072635.t1 AT4G30980 55.357 56 25 0 174 229 189 244 1.16e-15 77.4
MS.gene072635.t1 AT5G58010 43.373 83 41 2 148 229 88 165 1.65e-15 76.3
MS.gene072635.t1 AT4G28811 50.588 85 41 1 142 226 331 414 3.36e-13 70.9
MS.gene072635.t1 AT5G61270 41.489 94 55 0 134 227 43 136 4.52e-13 68.9
MS.gene072635.t1 AT5G61270 41.489 94 55 0 134 227 43 136 4.52e-13 68.9
MS.gene072635.t1 AT5G61270 41.489 94 55 0 134 227 131 224 6.79e-13 69.3
MS.gene072635.t1 AT1G10120 32.231 121 68 3 128 234 158 278 1.66e-12 68.2
MS.gene072635.t1 AT1G10120 32.231 121 68 3 128 234 158 278 1.66e-12 68.2
MS.gene072635.t1 AT5G50915 38.462 91 54 2 149 237 121 211 2.96e-12 66.2
MS.gene072635.t1 AT5G50915 38.462 91 54 2 149 237 121 211 2.96e-12 66.2
MS.gene072635.t1 AT5G50915 39.326 89 52 2 149 235 121 209 4.01e-12 66.2
MS.gene072635.t1 AT5G50915 39.326 89 52 2 149 235 121 209 4.01e-12 66.2
MS.gene072635.t1 AT1G68920 43.939 66 36 1 174 238 313 378 1.28e-11 65.9
MS.gene072635.t1 AT1G68920 43.939 66 36 1 174 238 314 379 1.34e-11 65.9
MS.gene072635.t1 AT1G68920 43.939 66 36 1 174 238 314 379 1.34e-11 65.9
MS.gene072635.t1 AT1G68920 43.939 66 36 1 174 238 314 379 1.34e-11 65.9
MS.gene072635.t1 AT2G14760 31.148 122 65 4 139 256 219 325 1.42e-11 65.1
MS.gene072635.t1 AT4G36540 30.597 134 85 3 102 234 87 213 1.44e-11 64.7
MS.gene072635.t1 AT4G36540 30.597 134 85 3 102 234 87 213 1.61e-11 64.7
MS.gene072635.t1 AT1G32640 30.556 180 95 5 49 219 340 498 1.63e-11 65.9
MS.gene072635.t1 AT1G02340 56.863 51 21 1 177 226 141 191 1.69e-11 64.3
MS.gene072635.t1 AT1G02340 56.863 51 21 1 177 226 134 184 1.82e-11 64.3
MS.gene072635.t1 AT5G43175 35.366 82 46 1 174 255 143 217 2.14e-11 63.2
MS.gene072635.t1 AT2G14760 33.333 87 49 2 172 256 259 338 2.14e-11 64.7
MS.gene072635.t1 AT1G26260 42.424 66 37 1 174 238 212 277 2.55e-11 64.7
MS.gene072635.t1 AT1G26260 42.424 66 37 1 174 238 234 299 2.90e-11 64.3
MS.gene072635.t1 AT1G26260 42.424 66 37 1 174 238 234 299 2.90e-11 64.3
MS.gene072635.t1 AT1G26260 42.424 66 37 1 174 238 234 299 2.90e-11 64.3
MS.gene072635.t1 AT1G26260 42.424 66 37 1 174 238 234 299 2.90e-11 64.3
MS.gene072635.t1 AT1G26260 42.424 66 37 1 174 238 183 248 2.90e-11 64.3
MS.gene072635.t1 AT4G34530 41.176 68 39 1 174 240 183 250 2.95e-11 63.9
MS.gene072635.t1 AT5G62610 49.091 55 27 1 174 227 164 218 3.00e-11 63.5
MS.gene072635.t1 AT5G62610 49.091 55 27 1 174 227 164 218 3.00e-11 63.5
MS.gene072635.t1 AT2G18300 43.548 62 34 1 174 234 196 257 3.71e-11 63.9
MS.gene072635.t1 AT2G18300 43.548 62 34 1 174 234 196 257 3.85e-11 63.9
MS.gene072635.t1 AT3G23690 42.424 66 37 1 174 238 202 267 4.22e-11 63.9
MS.gene072635.t1 AT1G59640 45.455 55 29 1 174 227 147 201 4.60e-11 62.8
MS.gene072635.t1 AT1G25330 49.091 55 27 1 174 227 115 169 4.77e-11 62.4
MS.gene072635.t1 AT2G18300 43.548 62 34 1 174 234 196 257 5.36e-11 63.5
MS.gene072635.t1 AT5G48560 47.273 55 28 1 174 227 312 366 8.53e-11 63.2
MS.gene072635.t1 AT3G07340 35.644 101 63 2 129 227 223 323 8.85e-11 63.2
MS.gene072635.t1 AT5G48560 47.273 55 28 1 174 227 312 366 9.10e-11 63.2
MS.gene072635.t1 AT2G46510 50.000 52 26 0 174 225 396 447 9.24e-11 63.2

Find 66 sgRNAs with CRISPR-Local

Find 151 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CACAACATTTGATGGGTTTC+AGG 0.156734 7.4:-5203980 MS.gene072635:CDS
TTTCTCTTTCCTCTAATTCT+AGG 0.178319 7.4:+5205775 None:intergenic
GACCCTCAAGCTTCAGTTAC+AGG 0.189644 7.4:-5204203 MS.gene072635:intron
TCACATACCTTATTGCAATT+TGG 0.224679 7.4:+5204383 None:intergenic
TTGACATGTGTGGTGGTAAT+TGG 0.291651 7.4:+5203871 None:intergenic
ACACCAACACCAACCTCTTT+TGG 0.299511 7.4:-5203801 MS.gene072635:CDS
CCTTTCATTCCTCCTATCAT+TGG 0.324461 7.4:-5203837 MS.gene072635:CDS
CTTACATCATCACTTAAATA+TGG 0.331025 7.4:+5205637 None:intergenic
ATGTCCTTCCAGGTTGATTC+TGG 0.340577 7.4:+5203775 None:intergenic
GACTAGCCACATGTCCTTCC+AGG 0.347583 7.4:+5203765 None:intergenic
ACCAATGCACCTAGAATTAG+AGG 0.347789 7.4:-5205784 MS.gene072635:CDS
CCATCACCCATTTGTGGAAT+AGG 0.357478 7.4:+5203942 None:intergenic
AACATCATAAAATGATTAAC+TGG 0.360190 7.4:+5204047 None:intergenic
TTCATATGCAGAGTTTGATT+TGG 0.364667 7.4:+5205864 None:intergenic
AACGACAGAGTTCAGATGTT+TGG 0.380475 7.4:-5203903 MS.gene072635:CDS
TATGAATGCACAACATTTGA+TGG 0.383363 7.4:-5203988 MS.gene072635:CDS
ACAGTTTCCTATTCCACAAA+TGG 0.388703 7.4:-5203949 MS.gene072635:CDS
TCCTCTAATTCTAGGTGCAT+TGG 0.390333 7.4:+5205783 None:intergenic
GCTCATACCAAATTGCAATA+AGG 0.391698 7.4:-5204390 MS.gene072635:intron
GACGTTGCAGATTATGTCAA+TGG 0.409902 7.4:-5204087 MS.gene072635:intron
ACCAAAGTTTAGATCATTTG+AGG 0.426290 7.4:+5205693 None:intergenic
AATTCATAATTTATCTGAAA+GGG 0.436637 7.4:-5205358 MS.gene072635:intron
GCCTCAAATGATCTAAACTT+TGG 0.436672 7.4:-5205694 MS.gene072635:CDS
TTGATTCTGGTTACCAAAAG+AGG 0.453744 7.4:+5203788 None:intergenic
ACTCTGTTTCCATTATGAGC+TGG 0.454011 7.4:+5205405 None:intergenic
AAAGAAAAGCCAGCTCATAA+TGG 0.458159 7.4:-5205414 MS.gene072635:CDS
AGGAGGAATGAAAGGTGCAT+TGG 0.466560 7.4:+5203845 None:intergenic
GGAGGAATGAAAGGTGCATT+GGG 0.473309 7.4:+5203846 None:intergenic
GTGGAAGAGTTTCCAATGAT+AGG 0.480699 7.4:+5203825 None:intergenic
TACCTGTAACTGAAGCTTGA+GGG 0.481997 7.4:+5204201 None:intergenic
GTGACACCATCACCCATTTG+TGG 0.484725 7.4:+5203936 None:intergenic
CAGTTTCCTATTCCACAAAT+GGG 0.491361 7.4:-5203948 MS.gene072635:CDS
TAATTCTAGGTGCATTGGTA+TGG 0.508040 7.4:+5205788 None:intergenic
TGAAGAAACACAAGAAAATA+CGG 0.510993 7.4:-5205439 MS.gene072635:CDS
TGGTGTTAGAAAATCATATG+AGG 0.518657 7.4:-5205674 MS.gene072635:CDS
TCAAGTGAGCATACAGAAGA+TGG 0.531560 7.4:+5205718 None:intergenic
ACAGAGTCAAGAGAAGCTAC+AGG 0.536275 7.4:-5205389 MS.gene072635:CDS
ATGAATGCACAACATTTGAT+GGG 0.539585 7.4:-5203987 MS.gene072635:CDS
AAATTCATAATTTATCTGAA+AGG 0.542829 7.4:-5205359 MS.gene072635:CDS
ACGTTGCAGATTATGTCAAT+GGG 0.542904 7.4:-5204086 MS.gene072635:intron
AGTACTATTCTTACTTTGGG+AGG 0.546973 7.4:+5206140 None:intergenic
GGAATAGGAAACTGTATTTG+TGG 0.547372 7.4:+5203957 None:intergenic
CATGTGTGGTGGTAATTGGT+TGG 0.548002 7.4:+5203875 None:intergenic
AGAGGTTGGTGTTGGTGTGG+TGG 0.551444 7.4:+5203806 None:intergenic
TTCATGTTCATATGATGTGC+GGG 0.552429 7.4:+5204023 None:intergenic
ATGACAAGAAGCAATGGCCT+AGG 0.554224 7.4:-5206186 MS.gene072635:CDS
CCTATTCCACAAATGGGTGA+TGG 0.561913 7.4:-5203942 MS.gene072635:CDS
TTCTGGTTACCAAAAGAGGT+TGG 0.567323 7.4:+5203792 None:intergenic
TCAGAATGTTCATCAACGGT+TGG 0.575923 7.4:+5205823 None:intergenic
AGCTTCAGAATGTTCATCAA+CGG 0.575933 7.4:+5205819 None:intergenic
TTTGGTAACCAGAATCAACC+TGG 0.577623 7.4:-5203783 MS.gene072635:CDS
TTACCTGTAACTGAAGCTTG+AGG 0.590352 7.4:+5204200 None:intergenic
ACTTAAATATGGAGAGTCAT+CGG 0.599086 7.4:+5205648 None:intergenic
ATTCATGTTCATATGATGTG+CGG 0.605271 7.4:+5204022 None:intergenic
CCAATGATAGGAGGAATGAA+AGG 0.605339 7.4:+5203837 None:intergenic
AGGTGCATTGGTATGGCTAG+AGG 0.613493 7.4:+5205795 None:intergenic
TCAGAGATGACAAGAAGCAA+TGG 0.621101 7.4:-5206192 MS.gene072635:CDS
GGAATGAAAATCACTGTGAG+AGG 0.626133 7.4:+5206161 None:intergenic
GAAGAGTTTCCAATGATAGG+AGG 0.626184 7.4:+5203828 None:intergenic
AATCACTGTGAGAGGAACCT+AGG 0.630251 7.4:+5206169 None:intergenic
TTACCAAAAGAGGTTGGTGT+TGG 0.647408 7.4:+5203798 None:intergenic
GTAACCAGAATCAACCTGGA+AGG 0.666181 7.4:-5203779 MS.gene072635:CDS
AAAAGAGGTTGGTGTTGGTG+TGG 0.666327 7.4:+5203803 None:intergenic
AATCAACCTGGAAGGACATG+TGG 0.668906 7.4:-5203771 MS.gene072635:CDS
TTGGGAATTGACATGTGTGG+TGG 0.729000 7.4:+5203864 None:intergenic
GCATTGGGAATTGACATGTG+TGG 0.733578 7.4:+5203861 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATAAGTTATATGAAATTAA+AGG + chr7.4:5205502-5205521 None:intergenic 10.0%
!! AAATTCATAATTTATCTGAA+AGG - chr7.4:5204618-5204637 MS.gene072635:intron 15.0%
!! AATTCATAATTTATCTGAAA+GGG - chr7.4:5204619-5204638 MS.gene072635:intron 15.0%
!! GAAAAAAAAAACTAATTAAG+AGG + chr7.4:5205067-5205086 None:intergenic 15.0%
!! AACATCATAAAATGATTAAC+TGG + chr7.4:5205933-5205952 None:intergenic 20.0%
!! AATAGAAGAGTTAAACAATT+TGG + chr7.4:5205103-5205122 None:intergenic 20.0%
!! ATTGTTGCATAGAAAATTTA+AGG + chr7.4:5205813-5205832 None:intergenic 20.0%
!! CATGATTAAAATTGAACAAT+AGG + chr7.4:5205133-5205152 None:intergenic 20.0%
!!! CTATTGTTCAATTTTAATCA+TGG - chr7.4:5205131-5205150 MS.gene072635:intron 20.0%
!!! TTCTGTATGCAATTTAATTT+CGG - chr7.4:5204694-5204713 MS.gene072635:intron 20.0%
! AATAAAGTTCAACTTCAACA+AGG + chr7.4:5205655-5205674 None:intergenic 25.0%
! CTTACATCATCACTTAAATA+TGG + chr7.4:5204343-5204362 None:intergenic 25.0%
! GTGAAATTGATAAATTGGAA+TGG - chr7.4:5204912-5204931 MS.gene072635:intron 25.0%
! TACAAACGTAAAATAGCATA+TGG + chr7.4:5204380-5204399 None:intergenic 25.0%
! TGAAGAAACACAAGAAAATA+CGG - chr7.4:5204538-5204557 MS.gene072635:intron 25.0%
! TTTGTAATTAAACAAAGACG+TGG - chr7.4:5204974-5204993 MS.gene072635:intron 25.0%
!! TATTTAAGTGATGATGTAAG+TGG - chr7.4:5204343-5204362 MS.gene072635:intron 25.0%
!! TCTGTATGCAATTTAATTTC+GGG - chr7.4:5204695-5204714 MS.gene072635:intron 25.0%
!!! TGCTTTGTTTTTGTTATAGA+TGG - chr7.4:5205711-5205730 MS.gene072635:CDS 25.0%
ACCAAAGTTTAGATCATTTG+AGG + chr7.4:5204287-5204306 None:intergenic 30.0%
ACTTAAATATGGAGAGTCAT+CGG + chr7.4:5204332-5204351 None:intergenic 30.0%
ATAATTTATCTGAAAGGGTG+AGG - chr7.4:5204624-5204643 MS.gene072635:intron 30.0%
ATGAATGCACAACATTTGAT+GGG - chr7.4:5205990-5206009 MS.gene072635:intron 30.0%
ATTCATGTTCATATGATGTG+CGG + chr7.4:5205958-5205977 None:intergenic 30.0%
CAATTTATCAATTTCACGTG+AGG + chr7.4:5204909-5204928 None:intergenic 30.0%
CTCACGTGAAATTGATAAAT+TGG - chr7.4:5204907-5204926 MS.gene072635:intron 30.0%
GGGTGTGAATATCAAAAAAA+TGG - chr7.4:5203950-5203969 MS.gene072635:CDS 30.0%
GTGAGTACTATTCTTACTTT+GGG + chr7.4:5203843-5203862 None:intergenic 30.0%
TATGAATGCACAACATTTGA+TGG - chr7.4:5205989-5206008 MS.gene072635:intron 30.0%
TCACATACCTTATTGCAATT+TGG + chr7.4:5205597-5205616 None:intergenic 30.0%
TGTGACAGAATTTGAATAAC+AGG + chr7.4:5205001-5205020 None:intergenic 30.0%
TGTGAGTACTATTCTTACTT+TGG + chr7.4:5203844-5203863 None:intergenic 30.0%
TTCATATGCAGAGTTTGATT+TGG + chr7.4:5204116-5204135 None:intergenic 30.0%
TTTCTCTTTCCTCTAATTCT+AGG + chr7.4:5204205-5204224 None:intergenic 30.0%
! TGGTGTTAGAAAATCATATG+AGG - chr7.4:5204303-5204322 MS.gene072635:intron 30.0%
!! CTTCTTTTGATTCTTTCTCT+TGG + chr7.4:5204042-5204061 None:intergenic 30.0%
AAAGAAAAGCCAGCTCATAA+TGG - chr7.4:5204563-5204582 MS.gene072635:intron 35.0%
ACAGTTTCCTATTCCACAAA+TGG - chr7.4:5206028-5206047 MS.gene072635:intron 35.0%
ACGTTGCAGATTATGTCAAT+GGG - chr7.4:5205891-5205910 MS.gene072635:CDS 35.0%
AGCTTCAGAATGTTCATCAA+CGG + chr7.4:5204161-5204180 None:intergenic 35.0%
AGGAAAACTGTATGAGTTCA+CGG + chr7.4:5205047-5205066 None:intergenic 35.0%
AGTACTATTCTTACTTTGGG+AGG + chr7.4:5203840-5203859 None:intergenic 35.0%
ATCAAGCTTCGTAAGAATGA+TGG + chr7.4:5204497-5204516 None:intergenic 35.0%
CAGTTTCCTATTCCACAAAT+GGG - chr7.4:5206029-5206048 MS.gene072635:intron 35.0%
CGAAGACAAATCAACGATTT+CGG - chr7.4:5205194-5205213 MS.gene072635:intron 35.0%
GCCTCAAATGATCTAAACTT+TGG - chr7.4:5204283-5204302 MS.gene072635:intron 35.0%
GCTCATACCAAATTGCAATA+AGG - chr7.4:5205587-5205606 MS.gene072635:intron 35.0%
GGAATAGGAAACTGTATTTG+TGG + chr7.4:5206023-5206042 None:intergenic 35.0%
TCACAGAAACAGTACTTGAT+GGG - chr7.4:5203929-5203948 MS.gene072635:CDS 35.0%
TCCTCTCAAAAAACCAAGAT+TGG - chr7.4:5203880-5203899 MS.gene072635:CDS 35.0%
TGTTTCGGTAGATTGTTGAT+TGG + chr7.4:5205162-5205181 None:intergenic 35.0%
TTCATGTTCATATGATGTGC+GGG + chr7.4:5205957-5205976 None:intergenic 35.0%
! TAATTCTAGGTGCATTGGTA+TGG + chr7.4:5204192-5204211 None:intergenic 35.0%
! TTGATTCTGGTTACCAAAAG+AGG + chr7.4:5206192-5206211 None:intergenic 35.0%
!! GTTTGGTTTGAATCCAATCT+TGG + chr7.4:5203896-5203915 None:intergenic 35.0%
!!! TCCAATCTTGGTTTTTTGAG+AGG + chr7.4:5203884-5203903 None:intergenic 35.0%
AAACTGTATGAGTTCACGGA+AGG + chr7.4:5205043-5205062 None:intergenic 40.0%
AACGACAGAGTTCAGATGTT+TGG - chr7.4:5206074-5206093 MS.gene072635:intron 40.0%
ACCAATGCACCTAGAATTAG+AGG - chr7.4:5204193-5204212 MS.gene072635:intron 40.0%
ACTCTGTTTCCATTATGAGC+TGG + chr7.4:5204575-5204594 None:intergenic 40.0%
AGGGAGAAGATTCACACATT+TGG - chr7.4:5205359-5205378 MS.gene072635:CDS 40.0%
AGGGAGAAGATTCACACATT+TGG + chr7.4:5205378-5205359 None:intergenic 40.0%
CACAGAAACAGTACTTGATG+GGG - chr7.4:5203930-5203949 MS.gene072635:CDS 40.0%
CAGATTCGTTTGTGGTGAAA+GGG - chr7.4:5205288-5205307 MS.gene072635:intron 40.0%
CCAATGATAGGAGGAATGAA+AGG + chr7.4:5206143-5206162 None:intergenic 40.0%
CCTTTCATTCCTCCTATCAT+TGG - chr7.4:5206140-5206159 MS.gene072635:intron 40.0%
CTCACAGAAACAGTACTTGA+TGG - chr7.4:5203928-5203947 MS.gene072635:CDS 40.0%
GAAGAGTTTCCAATGATAGG+AGG + chr7.4:5206152-5206171 None:intergenic 40.0%
GACGTTGCAGATTATGTCAA+TGG - chr7.4:5205890-5205909 MS.gene072635:CDS 40.0%
GGAATGAAAATCACTGTGAG+AGG + chr7.4:5203819-5203838 None:intergenic 40.0%
GTGGAAGAGTTTCCAATGAT+AGG + chr7.4:5206155-5206174 None:intergenic 40.0%
TACCTGTAACTGAAGCTTGA+GGG + chr7.4:5205779-5205798 None:intergenic 40.0%
TCAAGTGAGCATACAGAAGA+TGG + chr7.4:5204262-5204281 None:intergenic 40.0%
TCAGAATGTTCATCAACGGT+TGG + chr7.4:5204157-5204176 None:intergenic 40.0%
TCAGAGATGACAAGAAGCAA+TGG - chr7.4:5203785-5203804 MS.gene072635:CDS 40.0%
TCCTCTAATTCTAGGTGCAT+TGG + chr7.4:5204197-5204216 None:intergenic 40.0%
TTACCTGTAACTGAAGCTTG+AGG + chr7.4:5205780-5205799 None:intergenic 40.0%
TTACTATGAAGCACGAACAC+TGG - chr7.4:5204405-5204424 MS.gene072635:CDS 40.0%
TTGACATGTGTGGTGGTAAT+TGG + chr7.4:5206109-5206128 None:intergenic 40.0%
! TTCTGGTTACCAAAAGAGGT+TGG + chr7.4:5206188-5206207 None:intergenic 40.0%
! TTTGGTAACCAGAATCAACC+TGG - chr7.4:5206194-5206213 MS.gene072635:CDS 40.0%
!! ATTTTTACCATGCTGCTTGC+TGG + chr7.4:5204879-5204898 None:intergenic 40.0%
!! CACAACATTTGATGGGTTTC+AGG - chr7.4:5205997-5206016 MS.gene072635:intron 40.0%
!! CATTTGGATTTGCTGTGTTG+TGG - chr7.4:5205375-5205394 MS.gene072635:CDS 40.0%
!! CATTTGGATTTGCTGTGTTG+TGG + chr7.4:5205394-5205375 None:intergenic 40.0%
!! GAAGCATTGCTTTGATCGAA+TGG - chr7.4:5205419-5205438 MS.gene072635:CDS 40.0%
!! GAAGCATTGCTTTGATCGAA+TGG + chr7.4:5205438-5205419 None:intergenic 40.0%
!! GTGAGTTTGTTGTTGCAGTT+TGG + chr7.4:5203913-5203932 None:intergenic 40.0%
!! GTTGATTTGAGAAACACTGC+AGG + chr7.4:5204004-5204023 None:intergenic 40.0%
!! TTACCAAAAGAGGTTGGTGT+TGG + chr7.4:5206182-5206201 None:intergenic 40.0%
!! TTTTTACCATGCTGCTTGCT+GGG + chr7.4:5204878-5204897 None:intergenic 40.0%
AACCGAAGCAGATTCGTTTG+TGG - chr7.4:5205280-5205299 MS.gene072635:intron 45.0%
AATCAACCTGGAAGGACATG+TGG - chr7.4:5206206-5206225 MS.gene072635:CDS 45.0%
AATCACTGTGAGAGGAACCT+AGG + chr7.4:5203811-5203830 None:intergenic 45.0%
ACAGAGTCAAGAGAAGCTAC+AGG - chr7.4:5204588-5204607 MS.gene072635:intron 45.0%
ACGATTCCAGAAGACAACAG+AGG - chr7.4:5205241-5205260 MS.gene072635:intron 45.0%
ATGACAAGAAGCAATGGCCT+AGG - chr7.4:5203791-5203810 MS.gene072635:CDS 45.0%
CAAACGAATCTGCTTCGGTT+AGG + chr7.4:5205280-5205299 None:intergenic 45.0%
CACCACAAACGAATCTGCTT+CGG + chr7.4:5205285-5205304 None:intergenic 45.0%
CATGTGTGGTGGTAATTGGT+TGG + chr7.4:5206105-5206124 None:intergenic 45.0%
CCATCACCCATTTGTGGAAT+AGG + chr7.4:5206038-5206057 None:intergenic 45.0%
CCTATTCCACAAATGGGTGA+TGG - chr7.4:5206035-5206054 MS.gene072635:intron 45.0%
CGATTCCAGAAGACAACAGA+GGG - chr7.4:5205242-5205261 MS.gene072635:intron 45.0%
GCAGATTCGTTTGTGGTGAA+AGG - chr7.4:5205287-5205306 MS.gene072635:intron 45.0%
GCATTGGGAATTGACATGTG+TGG + chr7.4:5206119-5206138 None:intergenic 45.0%
GTAACCAGAATCAACCTGGA+AGG - chr7.4:5206198-5206217 MS.gene072635:CDS 45.0%
TCGCGAGTGGAATCATGTTT+CGG + chr7.4:5205177-5205196 None:intergenic 45.0%
TTGGAGACTTGGAGGAGAAA+GGG - chr7.4:5205340-5205359 MS.gene072635:intron 45.0%
TTGGAGACTTGGAGGAGAAA+GGG + chr7.4:5205359-5205340 None:intergenic 45.0%
TTGGGAATTGACATGTGTGG+TGG + chr7.4:5206116-5206135 None:intergenic 45.0%
! ACACCAACACCAACCTCTTT+TGG - chr7.4:5206176-5206195 MS.gene072635:CDS 45.0%
! AGGAGGAATGAAAGGTGCAT+TGG + chr7.4:5206135-5206154 None:intergenic 45.0%
! GGAGGAATGAAAGGTGCATT+GGG + chr7.4:5206134-5206153 None:intergenic 45.0%
!! AAAAGAGGTTGGTGTTGGTG+TGG + chr7.4:5206177-5206196 None:intergenic 45.0%
!! ATGTCCTTCCAGGTTGATTC+TGG + chr7.4:5206205-5206224 None:intergenic 45.0%
!! TCTGTTGTCTTCTGGAATCG+TGG + chr7.4:5205242-5205261 None:intergenic 45.0%
AACCCAACATAGCTGCTTGC+TGG - chr7.4:5205316-5205335 MS.gene072635:intron 50.0%
AACCCAGCAAGCAGCTATGT+TGG + chr7.4:5205322-5205341 None:intergenic 50.0%
AAGGCGGTGGGAGTAATAGA+TGG + chr7.4:5205024-5205043 None:intergenic 50.0%
ACCCAACATAGCTGCTTGCT+GGG - chr7.4:5205317-5205336 MS.gene072635:intron 50.0%
ACCCAGCAAGCAGCTATGTT+GGG + chr7.4:5205321-5205340 None:intergenic 50.0%
ACTGTGTCTGTGTCTGTGTC+TGG + chr7.4:5204443-5204462 None:intergenic 50.0%
CCAGAAGACAACAGAGGGTA+CGG - chr7.4:5205247-5205266 MS.gene072635:intron 50.0%
CTGTATGAGTTCACGGAAGG+CGG + chr7.4:5205040-5205059 None:intergenic 50.0%
GACCCTCAAGCTTCAGTTAC+AGG - chr7.4:5205774-5205793 MS.gene072635:CDS 50.0%
GTGACACCATCACCCATTTG+TGG + chr7.4:5206044-5206063 None:intergenic 50.0%
GTTGGAGACTTGGAGGAGAA+AGG - chr7.4:5205339-5205358 MS.gene072635:intron 50.0%
GTTGGAGACTTGGAGGAGAA+AGG + chr7.4:5205358-5205339 None:intergenic 50.0%
TGGAATGGAGCCTTCCACAA+CGG - chr7.4:5204927-5204946 MS.gene072635:intron 50.0%
! AACATAGCTGCTTGCTGGGT+TGG - chr7.4:5205321-5205340 MS.gene072635:intron 50.0%
! AGGTGCATTGGTATGGCTAG+AGG + chr7.4:5204185-5204204 None:intergenic 50.0%
!! CGTTGATTTGTCTTCGCGAG+TGG + chr7.4:5205190-5205209 None:intergenic 50.0%
AACGGGAGGTGGAGGAGAAA+AGG - chr7.4:5204945-5204964 MS.gene072635:intron 55.0%
ATGAGTTCACGGAAGGCGGT+GGG + chr7.4:5205036-5205055 None:intergenic 55.0%
CCGTACCCTCTGTTGTCTTC+TGG + chr7.4:5205250-5205269 None:intergenic 55.0%
GGAATGGAGCCTTCCACAAC+GGG - chr7.4:5204928-5204947 MS.gene072635:intron 55.0%
TATGAGTTCACGGAAGGCGG+TGG + chr7.4:5205037-5205056 None:intergenic 55.0%
! TACCATGCTGCTTGCTGGGT+TGG + chr7.4:5204874-5204893 None:intergenic 55.0%
! TGCTTGCTGGGTTGGAGACT+TGG - chr7.4:5205329-5205348 MS.gene072635:intron 55.0%
! TGCTTGCTGGGTTGGAGACT+TGG + chr7.4:5205348-5205329 None:intergenic 55.0%
! TTGCTGGGTTGGAGACTTGG+AGG - chr7.4:5205332-5205351 MS.gene072635:intron 55.0%
! TTGCTGGGTTGGAGACTTGG+AGG + chr7.4:5205351-5205332 None:intergenic 55.0%
!! AGAGGTTGGTGTTGGTGTGG+TGG + chr7.4:5206174-5206193 None:intergenic 55.0%
!! TCTTCTGGAATCGTGGCTGC+CGG + chr7.4:5205235-5205254 None:intergenic 55.0%
ATGGAGCCTTCCACAACGGG+AGG - chr7.4:5204931-5204950 MS.gene072635:intron 60.0%
CTCCAACCCAGCAAGCAGCA+TGG - chr7.4:5204869-5204888 MS.gene072635:intron 60.0%
TTCTCCTCCACCTCCCGTTG+TGG + chr7.4:5204944-5204963 None:intergenic 60.0%
!! TCGGATTGCGAGAGTGTCGC+CGG - chr7.4:5205213-5205232 MS.gene072635:intron 60.0%
CCTCCACCTCCCGTTGTGGA+AGG + chr7.4:5204940-5204959 None:intergenic 65.0%
CCTTCCACAACGGGAGGTGG+AGG - chr7.4:5204937-5204956 MS.gene072635:intron 65.0%
GAGCCTTCCACAACGGGAGG+TGG - chr7.4:5204934-5204953 MS.gene072635:intron 65.0%


Chromosome Type Strat End Strand Name
chr7.4 gene 5203767 5206232 5203767 ID=MS.gene072635
chr7.4 mRNA 5203767 5206232 5203767 ID=MS.gene072635.t1;Parent=MS.gene072635
chr7.4 exon 5206154 5206232 5206154 ID=MS.gene072635.t1.exon1;Parent=MS.gene072635.t1
chr7.4 CDS 5206154 5206232 5206154 ID=cds.MS.gene072635.t1;Parent=MS.gene072635.t1
chr7.4 exon 5205642 5205991 5205642 ID=MS.gene072635.t1.exon2;Parent=MS.gene072635.t1
chr7.4 CDS 5205642 5205991 5205642 ID=cds.MS.gene072635.t1;Parent=MS.gene072635.t1
chr7.4 exon 5205359 5205466 5205359 ID=MS.gene072635.t1.exon3;Parent=MS.gene072635.t1
chr7.4 CDS 5205359 5205466 5205359 ID=cds.MS.gene072635.t1;Parent=MS.gene072635.t1
chr7.4 exon 5204391 5204456 5204391 ID=MS.gene072635.t1.exon4;Parent=MS.gene072635.t1
chr7.4 CDS 5204391 5204456 5204391 ID=cds.MS.gene072635.t1;Parent=MS.gene072635.t1
chr7.4 exon 5204204 5204269 5204204 ID=MS.gene072635.t1.exon5;Parent=MS.gene072635.t1
chr7.4 CDS 5204204 5204269 5204204 ID=cds.MS.gene072635.t1;Parent=MS.gene072635.t1
chr7.4 exon 5203767 5204099 5203767 ID=MS.gene072635.t1.exon6;Parent=MS.gene072635.t1
chr7.4 CDS 5203767 5204099 5203767 ID=cds.MS.gene072635.t1;Parent=MS.gene072635.t1
Gene Sequence

>MS.gene072635

ATGTTTGATTCTAGCAACTCAGAGATGACAAGAAGCAATGGCCTAGGTTCCTCTCACAGTGATTTTCATTCCTCCCAAAGTAAGAATAGTACTCACATGTTCACATATTTAACTCCTCTCAAAAAACCAAGATTGGATTCAAACCAAACTGCAACAACAAACTCACAGAAACAGTACTTGATGGGGTGTGAATATCAAAAAAATGGAAAAGTGAACTTCTCAAACTTTTCTATACCTGCAGTGTTTCTCAAATCAACTCATCAACAAAGAGTCCAAGAGAAAGAATCAAAAGAAGTTGCTTCTACAAAATTTCAACAACAAAAACACTTAACTTTAAATGCTGCTACCAAATCAAACTCTGCATATGAAAGAAGTAGAGAATTACTACCAACCGTTGATGAACATTCTGAAGCTGCCTCTAGCCATACCAATGCACCTAGAATTAGAGGAAAGAGAAAAGCTAGTGCTATTAACTTATGTAATGCACGAGAACCATCTTCTGTATGCTCACTTGAAGCCTCAAATGATCTAAACTTTGGTGTTAGAAAATCATATGAGGACACCGATGACTCTCCATATTTAAGTGATGATGTAAGTGGAATTCAAGAAACCATATGCTATTTTACGTTTGTAGTTAGTTACTATGAAGCACGAACACTGGACACGACACGTACCAGACACAGACACAGACACAGTTTCAATATGAAGTGTTATGTTATATTAGTTACCATCATTCTTACGAAGCTTGATTGATTTGATGTTGCAGAATGATGAAGAAACACAAGAAAATACGGTGAAAGAAAAGCCAGCTCATAATGGAAACAGAGTCAAGAGAAGCTACAGGAATGCAAAAATTCATAATTTATCTGAAAGGGTGAGGAACTTTAAAATGAGTTAAAAAAAATAAAAATAAATATTTGTATGTATTTCTGTATGCAATTTAATTTCGGGTGTGATTCACAGCAGTCAAAATAATAATACAGTCATGCAGTCAAATTAATTACAGCCATTCGATCAAAGCAATGCTTCAGATTCACTCACACTTAAAATCCACAACACAGCAAATCCAAATGTGTGAATCTTCTCCCTTTCTCCTCCAAGTCTCCAACCCAGCAAGCAGCATGGTAAAAATTAAAATGCCTCACGTGAAATTGATAAATTGGAATGGAGCCTTCCACAACGGGAGGTGGAGGAGAAAAGGAACAATTTTGTAATTAAACAAAGACGTGGTCCTGTTATTCAAATTCTGTCACACCATCTATTACTCCCACCGCCTTCCGTGAACTCATACAGTTTTCCTCTTAATTAGTTTTTTTTTTCTTTCTTCATATTTCCAAATTGTTTAACTCTTCTATTATAGAATCCTATTGTTCAATTTTAATCATGGAGCAACCAATCAACAATCTACCGAAACATGATTCCACTCGCGAAGACAAATCAACGATTTCGGATTGCGAGAGTGTCGCCGGCAGCCACGATTCCAGAAGACAACAGAGGGTACGGATATCAACCTAACCGAAGCAGATTCGTTTGTGGTGAAAGGGATATCAACCCAACATAGCTGCTTGCTGGGTTGGAGACTTGGAGGAGAAAGGGAGAAGATTCACACATTTGGATTTGCTGTGTTGTGGATTTTAAGTGTGAGTGAATCTGAAGCATTGCTTTGATCGAATGGCTGTAATTAATTTGACTGCATGACTGTATTATTATTTTGACTGCTGTGAATCACATTCCTTTAATTTCATATAACTTATAAATATTTATTGATATTTTCAGAAGAGAAGAGATAAAATTAATGAGAAAATACGTGCATTGAAAGAGCTCATACCAAATTGCAATAAGGTATGTGAATATTAATTTATTTTGATTTTTGTAACAAGAAAGACCTTGTTGAAGTTGAACTTTATTTGTTTTAATTAATTAATTATATAAATATAAACGTTTTGCTTTGTTTTTGTTATAGATGGACAAAGCTTCAATGCTTGACGATGCAATCGATTATCTTAAGACCCTCAAGCTTCAGTTACAGGTAAAATTTTATGTCCTTAAATTTTCTATGCAACAATATTTAAATTGAGATTTCACTATATAATCTTATATTAAATATTAATTTTATGAATTATGACGTTGCAGATTATGTCAATGGGAAGAGCACTTTGTATGCCAGTTAATCATTTTATGATGTTACCCGCACATCATATGAACATGAATATGAATATGAATATGAATGCACAACATTTGATGGGTTTCAGGCCACAAATACAGTTTCCTATTCCACAAATGGGTGATGGTGTCACAAACAATAACAACGACAGAGTTCAGATGTTTGGTTTCTCCAACCAATTACCACCACACATGTCAATTCCCAATGCACCTTTCATTCCTCCTATCATTGGAAACTCTTCCACCACACCAACACCAACCTCTTTTGGTAACCAGAATCAACCTGGAAGGACATGTGGCTAG
Protein sequence

>MS.gene072635.t1

MFDSSNSEMTRSNGLGSSHSDFHSSQMFLKSTHQQRVQEKESKEVASTKFQQQKHLTLNAATKSNSAYERSRELLPTVDEHSEAASSHTNAPRIRGKRKASAINLCNAREPSSVCSLEASNDLNFGVRKSYEDTDDSPYLSDDNDEETQENTVKEKPAHNGNRVKRSYRNAKIHNLSERKRRDKINEKIRALKELIPNCNKMDKASMLDDAIDYLKTLKLQLQIMSMGRALCMPVNHFMMLPAHHMNMNMNMNMNAQHLMGFRPQIQFPIPQMGDGVTNNNNDRVQMFGFSNQLPPHMSIPNAPFIPPIIGNSSTTPTPTSFGNQNQPGRTCG