Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene073769.t1 | XP_003590505.1 | 93.7 | 222 | 14 | 0 | 1 | 222 | 1 | 222 | 4.60E-119 | 437.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene073769.t1 | P08688 | 44.6 | 222 | 115 | 6 | 6 | 220 | 7 | 227 | 2.9e-45 | 183.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene073769.t1 | G7IBL7 | 93.7 | 222 | 14 | 0 | 1 | 222 | 1 | 222 | 3.3e-119 | 437.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene054742 | MS.gene073769 | 0.823626 | 1.25E-53 | -1.69E-46 |
| MS.gene056633 | MS.gene073769 | -0.803917 | 2.77E-49 | -1.69E-46 |
| MS.gene060087 | MS.gene073769 | 0.841353 | 5.00E-58 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene073769.t1 | MTR_1g066530 | 93.694 | 222 | 14 | 0 | 1 | 222 | 1 | 222 | 7.68e-157 | 433 |
| MS.gene073769.t1 | MTR_6g073540 | 50.000 | 230 | 106 | 2 | 1 | 221 | 1 | 230 | 1.39e-74 | 225 |
| MS.gene073769.t1 | MTR_6g072470 | 51.500 | 200 | 91 | 1 | 1 | 194 | 1 | 200 | 1.28e-67 | 207 |
| MS.gene073769.t1 | MTR_6g048620 | 48.649 | 222 | 107 | 5 | 6 | 220 | 5 | 226 | 6.78e-65 | 201 |
| MS.gene073769.t1 | MTR_6g045077 | 45.000 | 220 | 116 | 3 | 4 | 220 | 3 | 220 | 4.33e-63 | 196 |
| MS.gene073769.t1 | MTR_6g048910 | 45.455 | 220 | 113 | 4 | 6 | 220 | 5 | 222 | 2.58e-62 | 194 |
| MS.gene073769.t1 | MTR_0695s0030 | 44.545 | 220 | 117 | 4 | 6 | 220 | 65 | 284 | 8.83e-62 | 195 |
| MS.gene073769.t1 | MTR_8g038100 | 45.000 | 220 | 116 | 4 | 6 | 220 | 281 | 500 | 1.48e-59 | 196 |
| MS.gene073769.t1 | MTR_8g038070 | 44.545 | 220 | 117 | 4 | 6 | 220 | 219 | 438 | 2.16e-59 | 193 |
| MS.gene073769.t1 | MTR_0057s0100 | 41.364 | 220 | 124 | 5 | 6 | 220 | 7 | 226 | 9.02e-54 | 172 |
| MS.gene073769.t1 | MTR_0057s0170 | 41.364 | 220 | 124 | 5 | 6 | 220 | 7 | 226 | 9.92e-53 | 169 |
| MS.gene073769.t1 | MTR_7g103990 | 41.704 | 223 | 120 | 8 | 6 | 220 | 7 | 227 | 4.09e-50 | 163 |
| MS.gene073769.t1 | MTR_0216s0030 | 40.404 | 198 | 110 | 3 | 4 | 197 | 1 | 194 | 1.04e-47 | 155 |
| MS.gene073769.t1 | MTR_0807s0020 | 37.500 | 224 | 130 | 5 | 1 | 220 | 1 | 218 | 3.17e-46 | 152 |
| MS.gene073769.t1 | MTR_6g040290 | 40.359 | 223 | 125 | 6 | 6 | 220 | 7 | 229 | 1.59e-45 | 151 |
| MS.gene073769.t1 | MTR_6g048880 | 40.449 | 178 | 96 | 5 | 50 | 220 | 4 | 178 | 1.42e-39 | 134 |
| MS.gene073769.t1 | MTR_7g012000 | 39.904 | 208 | 95 | 5 | 6 | 209 | 7 | 188 | 3.38e-39 | 134 |
| MS.gene073769.t1 | MTR_6g048810 | 50.355 | 141 | 64 | 4 | 84 | 220 | 162 | 300 | 6.79e-37 | 131 |
| MS.gene073769.t1 | MTR_4g102470 | 40.556 | 180 | 84 | 5 | 6 | 182 | 7 | 166 | 2.38e-33 | 119 |
| MS.gene073769.t1 | MTR_6g049040 | 40.146 | 137 | 65 | 3 | 1 | 135 | 1 | 122 | 5.73e-25 | 95.9 |
| MS.gene073769.t1 | MTR_6g049040 | 35.043 | 117 | 65 | 2 | 72 | 187 | 16 | 122 | 3.13e-16 | 72.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 44 sgRNAs with CRISPR-Local
Find 50 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTCGGTACGGTCTTTGAAAA+TGG | 0.203555 | 1.1:+49060488 | MS.gene073769:CDS |
| CTTGAACACGTAACATTCAT+AGG | 0.229009 | 1.1:-49060051 | None:intergenic |
| ATCCGGAGCGAAATCTATTT+TGG | 0.242862 | 1.1:-49060255 | None:intergenic |
| TTTAACAACACGATCCTTCT+TGG | 0.279901 | 1.1:-49060111 | None:intergenic |
| TAGATCTCTAGAAGTTTAAT+AGG | 0.303436 | 1.1:-49060661 | None:intergenic |
| ACTCATTACTCCATTCGTTC+TGG | 0.346885 | 1.1:+49060452 | MS.gene073769:CDS |
| AATGGTGTTTCATCAAGAAA+TGG | 0.395080 | 1.1:-49060161 | None:intergenic |
| TCCAGAGAAGATTATTGCTT+CGG | 0.411108 | 1.1:-49060222 | None:intergenic |
| AACATGGTAGTGATTGGAAC+AGG | 0.421529 | 1.1:-49060302 | None:intergenic |
| GTTACGTGTTCAAGAAGAAA+AGG | 0.439700 | 1.1:+49060060 | MS.gene073769:CDS |
| ACACGATCCTTCTTGGCTCC+TGG | 0.452509 | 1.1:-49060104 | None:intergenic |
| CAATGGAACATGGTAGTGAT+TGG | 0.462327 | 1.1:-49060308 | None:intergenic |
| GAATAAGAAAACATTATGTC+CGG | 0.469058 | 1.1:-49060381 | None:intergenic |
| TGATGAAACACCATTTGAAA+AGG | 0.482259 | 1.1:+49060169 | MS.gene073769:CDS |
| ACCGAAGCAATAATCTTCTC+TGG | 0.483775 | 1.1:+49060221 | MS.gene073769:CDS |
| TTTAATAGGCTCTGAAGAGC+AGG | 0.498729 | 1.1:-49060647 | None:intergenic |
| GTTCAAGAAGAAAAGGTTTG+CGG | 0.504927 | 1.1:+49060067 | MS.gene073769:CDS |
| AACAGGGCCATCGAGTAAAT+CGG | 0.507747 | 1.1:-49060285 | None:intergenic |
| TTGAACACGTAACATTCATA+GGG | 0.512326 | 1.1:-49060050 | None:intergenic |
| AGTAAATCGGTATCTGAATC+CGG | 0.514277 | 1.1:-49060272 | None:intergenic |
| GCGGTTGTGAATTATGCTCC+AGG | 0.516853 | 1.1:+49060086 | MS.gene073769:CDS |
| TGTCAATGGTAGAGTAAAGC+TGG | 0.518786 | 1.1:+49060610 | MS.gene073769:CDS |
| GACAATACAGTCGCCTTCGT+TGG | 0.522082 | 1.1:-49060591 | None:intergenic |
| GACGCTGCAATTAAGTGGAC+CGG | 0.527798 | 1.1:+49060362 | MS.gene073769:CDS |
| AAACCATCGGAAGTATGAAT+CGG | 0.529141 | 1.1:-49060137 | None:intergenic |
| TTCGGTGCCTTCTGTCTCAA+AGG | 0.532520 | 1.1:-49060204 | None:intergenic |
| GAATAGACGCTGCAATTAAG+TGG | 0.535911 | 1.1:+49060357 | MS.gene073769:CDS |
| AGGAAATGAGTATGTTCGTA+TGG | 0.536361 | 1.1:+49060406 | MS.gene073769:CDS |
| TACCATGTTCCATTGTCTCA+AGG | 0.539896 | 1.1:+49060316 | MS.gene073769:CDS |
| GAAGAGCAGGCCATTCATCA+TGG | 0.540017 | 1.1:-49060634 | None:intergenic |
| AAGCCGATTCATACTTCCGA+TGG | 0.550807 | 1.1:+49060134 | MS.gene073769:CDS |
| TAAAGCTGGTCCATGATGAA+TGG | 0.552708 | 1.1:+49060624 | MS.gene073769:CDS |
| AAAACGTTCCCTTGAGACAA+TGG | 0.561335 | 1.1:-49060325 | None:intergenic |
| TGCCAAAATAGATTTCGCTC+CGG | 0.565519 | 1.1:+49060253 | MS.gene073769:CDS |
| ATCAAGAAATGGAAAACCAT+CGG | 0.570603 | 1.1:-49060150 | None:intergenic |
| GATTGTGCCTTTGAGACAGA+AGG | 0.572261 | 1.1:+49060197 | MS.gene073769:CDS |
| ACCATGTTCCATTGTCTCAA+GGG | 0.576700 | 1.1:+49060317 | MS.gene073769:CDS |
| GAAATGCAGTATCAATGACT+TGG | 0.591249 | 1.1:-49060016 | None:intergenic |
| TTATGCTCCAGGAGCCAAGA+AGG | 0.592184 | 1.1:+49060097 | MS.gene073769:CDS |
| TCAGATACCGATTTACTCGA+TGG | 0.596226 | 1.1:+49060278 | MS.gene073769:CDS |
| GAAGGCGACTGTATTGTCAA+TGG | 0.604836 | 1.1:+49060596 | MS.gene073769:CDS |
| CGTATTGATATTTCCAACGA+AGG | 0.614900 | 1.1:+49060578 | MS.gene073769:CDS |
| TCCCTTGAGACAATGGAACA+TGG | 0.652701 | 1.1:-49060318 | None:intergenic |
| ACATGGTAGTGATTGGAACA+GGG | 0.671114 | 1.1:-49060301 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | CATAATGTTTTCTTATTCAA+AGG | + | chr1.1:49060386-49060405 | MS.gene073769:CDS | 20.0% |
| ! | GAATAAGAAAACATTATGTC+CGG | - | chr1.1:49060384-49060403 | None:intergenic | 25.0% |
| AATGGTGTTTCATCAAGAAA+TGG | - | chr1.1:49060164-49060183 | None:intergenic | 30.0% | |
| ATCAAGAAATGGAAAACCAT+CGG | - | chr1.1:49060153-49060172 | None:intergenic | 30.0% | |
| TGATGAAACACCATTTGAAA+AGG | + | chr1.1:49060169-49060188 | MS.gene073769:CDS | 30.0% | |
| TTGAACACGTAACATTCATA+GGG | - | chr1.1:49060053-49060072 | None:intergenic | 30.0% | |
| ! | AATCAAGCTTACGTTTTCAA+AGG | + | chr1.1:49060542-49060561 | MS.gene073769:CDS | 30.0% |
| ! | CAATCTACTTCCTTTTCAAA+TGG | - | chr1.1:49060182-49060201 | None:intergenic | 30.0% |
| !! | AAGGGAACGTTTTTAGAAAA+TGG | + | chr1.1:49060335-49060354 | MS.gene073769:CDS | 30.0% |
| !! | TTTTAAAAGTTTGCTCGGTA+CGG | + | chr1.1:49060475-49060494 | MS.gene073769:CDS | 30.0% |
| !!! | ACTTTTAAAACCAGAACGAA+TGG | - | chr1.1:49060465-49060484 | None:intergenic | 30.0% |
| !!! | TCTGGTTTTAAAAGTTTGCT+CGG | + | chr1.1:49060470-49060489 | MS.gene073769:CDS | 30.0% |
| AGGAAATGAGTATGTTCGTA+TGG | + | chr1.1:49060406-49060425 | MS.gene073769:CDS | 35.0% | |
| AGTAAATCGGTATCTGAATC+CGG | - | chr1.1:49060275-49060294 | None:intergenic | 35.0% | |
| CTTGAACACGTAACATTCAT+AGG | - | chr1.1:49060054-49060073 | None:intergenic | 35.0% | |
| GAAATGCAGTATCAATGACT+TGG | - | chr1.1:49060019-49060038 | None:intergenic | 35.0% | |
| GTTACGTGTTCAAGAAGAAA+AGG | + | chr1.1:49060060-49060079 | MS.gene073769:CDS | 35.0% | |
| GTTCAAGAAGAAAAGGTTTG+CGG | + | chr1.1:49060067-49060086 | MS.gene073769:CDS | 35.0% | |
| TCCAGAGAAGATTATTGCTT+CGG | - | chr1.1:49060225-49060244 | None:intergenic | 35.0% | |
| TTTAACAACACGATCCTTCT+TGG | - | chr1.1:49060114-49060133 | None:intergenic | 35.0% | |
| ! | AAACCATCGGAAGTATGAAT+CGG | - | chr1.1:49060140-49060159 | None:intergenic | 35.0% |
| ! | CGTATTGATATTTCCAACGA+AGG | + | chr1.1:49060578-49060597 | MS.gene073769:CDS | 35.0% |
| AAAACGTTCCCTTGAGACAA+TGG | - | chr1.1:49060328-49060347 | None:intergenic | 40.0% | |
| ACCATGTTCCATTGTCTCAA+GGG | + | chr1.1:49060317-49060336 | MS.gene073769:CDS | 40.0% | |
| ACCGAAGCAATAATCTTCTC+TGG | + | chr1.1:49060221-49060240 | MS.gene073769:CDS | 40.0% | |
| ACTCATTACTCCATTCGTTC+TGG | + | chr1.1:49060452-49060471 | MS.gene073769:CDS | 40.0% | |
| GAATAGACGCTGCAATTAAG+TGG | + | chr1.1:49060357-49060376 | MS.gene073769:CDS | 40.0% | |
| TAAAGCTGGTCCATGATGAA+TGG | + | chr1.1:49060624-49060643 | MS.gene073769:CDS | 40.0% | |
| TACCATGTTCCATTGTCTCA+AGG | + | chr1.1:49060316-49060335 | MS.gene073769:CDS | 40.0% | |
| TGCCAAAATAGATTTCGCTC+CGG | + | chr1.1:49060253-49060272 | MS.gene073769:CDS | 40.0% | |
| TGTCAATGGTAGAGTAAAGC+TGG | + | chr1.1:49060610-49060629 | MS.gene073769:CDS | 40.0% | |
| ! | AACATGGTAGTGATTGGAAC+AGG | - | chr1.1:49060305-49060324 | None:intergenic | 40.0% |
| ! | ACATGGTAGTGATTGGAACA+GGG | - | chr1.1:49060304-49060323 | None:intergenic | 40.0% |
| ! | ATCCGGAGCGAAATCTATTT+TGG | - | chr1.1:49060258-49060277 | None:intergenic | 40.0% |
| ! | CAATGGAACATGGTAGTGAT+TGG | - | chr1.1:49060311-49060330 | None:intergenic | 40.0% |
| !! | TCAGATACCGATTTACTCGA+TGG | + | chr1.1:49060278-49060297 | MS.gene073769:CDS | 40.0% |
| !! | TTTAATAGGCTCTGAAGAGC+AGG | - | chr1.1:49060650-49060669 | None:intergenic | 40.0% |
| AACAGGGCCATCGAGTAAAT+CGG | - | chr1.1:49060288-49060307 | None:intergenic | 45.0% | |
| GAAGGCGACTGTATTGTCAA+TGG | + | chr1.1:49060596-49060615 | MS.gene073769:CDS | 45.0% | |
| TCCCTTGAGACAATGGAACA+TGG | - | chr1.1:49060321-49060340 | None:intergenic | 45.0% | |
| ! | AAGCCGATTCATACTTCCGA+TGG | + | chr1.1:49060134-49060153 | MS.gene073769:CDS | 45.0% |
| ! | CTCGGTACGGTCTTTGAAAA+TGG | + | chr1.1:49060488-49060507 | MS.gene073769:CDS | 45.0% |
| !! | GATTGTGCCTTTGAGACAGA+AGG | + | chr1.1:49060197-49060216 | MS.gene073769:CDS | 45.0% |
| GAAGAGCAGGCCATTCATCA+TGG | - | chr1.1:49060637-49060656 | None:intergenic | 50.0% | |
| GACAATACAGTCGCCTTCGT+TGG | - | chr1.1:49060594-49060613 | None:intergenic | 50.0% | |
| GACGCTGCAATTAAGTGGAC+CGG | + | chr1.1:49060362-49060381 | MS.gene073769:CDS | 50.0% | |
| GCGGTTGTGAATTATGCTCC+AGG | + | chr1.1:49060086-49060105 | MS.gene073769:CDS | 50.0% | |
| TTATGCTCCAGGAGCCAAGA+AGG | + | chr1.1:49060097-49060116 | MS.gene073769:CDS | 50.0% | |
| !! | TTCGGTGCCTTCTGTCTCAA+AGG | - | chr1.1:49060207-49060226 | None:intergenic | 50.0% |
| ACACGATCCTTCTTGGCTCC+TGG | - | chr1.1:49060107-49060126 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.1 | gene | 49060008 | 49060676 | 49060008 | ID=MS.gene073769 |
| chr1.1 | mRNA | 49060008 | 49060676 | 49060008 | ID=MS.gene073769.t1;Parent=MS.gene073769 |
| chr1.1 | exon | 49060008 | 49060676 | 49060008 | ID=MS.gene073769.t1.exon1;Parent=MS.gene073769.t1 |
| chr1.1 | CDS | 49060008 | 49060676 | 49060008 | ID=cds.MS.gene073769.t1;Parent=MS.gene073769.t1 |
| Gene Sequence |
| Protein sequence |
