AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene056633


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene056633.t1 MTR_4g068770 98.667 150 2 0 2 151 113 262 8.77e-107 305
MS.gene056633.t1 MTR_3g032820 66.038 106 10 1 3 82 33 138 6.52e-40 131
MS.gene056633.t1 MTR_5g025750 47.101 138 57 4 2 134 124 250 3.35e-35 123
MS.gene056633.t1 MTR_3g032210 80.645 62 9 1 2 63 92 150 5.93e-29 105
MS.gene056633.t1 MTR_3g032770 82.759 58 8 1 2 57 7 64 1.47e-26 96.7
MS.gene056633.t1 MTR_3g032170 62.500 80 18 2 2 81 22 89 1.32e-25 94.0
MS.gene056633.t1 MTR_4g082590 32.432 148 92 2 2 149 152 291 2.86e-25 98.2
MS.gene056633.t1 MTR_3g032217 78.000 50 11 0 26 75 1 50 2.12e-20 79.3
MS.gene056633.t1 MTR_3g032100 67.925 53 16 1 34 85 3 55 2.64e-17 72.0
MS.gene056633.t1 MTR_3g032390 64.286 56 8 2 26 81 1 44 3.64e-13 60.8
MS.gene056633.t1 MTR_3g032405 68.182 44 5 1 36 79 8 42 1.48e-12 59.3
MS.gene056633.t1 MTR_3g032810 87.500 32 4 0 106 137 1 32 2.22e-11 55.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene056633.t1 AT5G13720 88.667 150 17 0 2 151 112 261 8.37e-96 277
MS.gene056633.t1 AT4G19390 53.741 147 62 1 2 148 42 182 3.17e-48 154
MS.gene056633.t1 AT4G19390 53.741 147 62 1 2 148 130 270 5.15e-47 154

Find 23 sgRNAs with CRISPR-Local

Find 40 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAAAGAGAGAGGATCCTTTA+AGG 0.208592 4.3:+36443483 None:intergenic
TCTGTCGACCGTGCCCTTAA+AGG 0.289306 4.3:-36443497 MS.gene056633:CDS
CTTAAAGGATCCTCTCTCTT+TGG 0.305772 4.3:-36443482 MS.gene056633:CDS
GCTGCAGATGTTTATCTTGC+TGG 0.317902 4.3:-36443593 MS.gene056633:intron
TTGAAGCATACAAAGTCTAT+TGG 0.342156 4.3:-36443745 MS.gene056633:CDS
CTTTGGAATGTTCGCTTTGA+AGG 0.345818 4.3:-36443465 MS.gene056633:intron
AAAGAGAGAGGATCCTTTAA+GGG 0.401841 4.3:+36443484 None:intergenic
CTGCTTCTAGTTGAAGCAAT+TGG 0.409753 4.3:-36443683 MS.gene056633:intron
AATGTTTGAGAGGAGCAAAA+TGG 0.444488 4.3:-36443263 MS.gene056633:CDS
GGTTGTCATTGCCACAGGAT+TGG 0.447202 4.3:-36443242 MS.gene056633:CDS
CTTGCTTATGCAGATTATGA+AGG 0.473431 4.3:+36443167 None:intergenic
AGGAGTTCACACTGGAAAGA+TGG 0.517911 4.3:-36443708 MS.gene056633:CDS
CGATGAACTGAAAACAAAAG+TGG 0.531141 4.3:-36443317 MS.gene056633:CDS
AAAATGGTTGTCATTGCCAC+AGG 0.534800 4.3:-36443247 MS.gene056633:CDS
GATGAACTGAAAACAAAAGT+GGG 0.560414 4.3:-36443316 MS.gene056633:CDS
TATTGGACAAGTTGTGTCAA+AGG 0.561661 4.3:-36443728 MS.gene056633:CDS
GAGAGGATCCTTTAAGGGCA+CGG 0.580531 4.3:+36443489 None:intergenic
TTAAGGGCACGGTCGACAGA+AGG 0.584565 4.3:+36443500 None:intergenic
ACAGAAGGTTGCACATCATG+AGG 0.628237 4.3:+36443515 None:intergenic
AGCGAACATTCCAAAGAGAG+AGG 0.634453 4.3:+36443472 None:intergenic
TGTGTCAAAGGAGTTCACAC+TGG 0.642348 4.3:-36443716 MS.gene056633:CDS
ACTTAGTAAATCCAATCCTG+TGG 0.659554 4.3:+36443231 None:intergenic
ATGAACTGAAAACAAAAGTG+GGG 0.689995 4.3:-36443315 MS.gene056633:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CTTTCTTGAATTCAATAAAA+TGG - chr4.3:36443399-36443418 MS.gene056633:intron 20.0%
!!! ATATATTCTCGATCATTTTA+GGG - chr4.3:36443245-36443264 MS.gene056633:CDS 20.0%
!!! CATTTTATTGAATTCAAGAA+AGG + chr4.3:36443401-36443420 None:intergenic 20.0%
!!! TATATATTCTCGATCATTTT+AGG - chr4.3:36443244-36443263 MS.gene056633:CDS 20.0%
! ATATCCAGTCAAATATATGA+AGG + chr4.3:36443227-36443246 None:intergenic 25.0%
! TTCTTGTAAAAATGTTTGAG+AGG - chr4.3:36443754-36443773 MS.gene056633:CDS 25.0%
ATGAACTGAAAACAAAAGTG+GGG - chr4.3:36443712-36443731 MS.gene056633:CDS 30.0%
GATGAACTGAAAACAAAAGT+GGG - chr4.3:36443711-36443730 MS.gene056633:CDS 30.0%
TTGAAGCATACAAAGTCTAT+TGG - chr4.3:36443282-36443301 MS.gene056633:CDS 30.0%
! TTAGCCTTCATATATTTGAC+TGG - chr4.3:36443220-36443239 MS.gene056633:CDS 30.0%
!!! GGTACTGTTATGTTGATTTT+TGG - chr4.3:36443455-36443474 MS.gene056633:intron 30.0%
!!! GTTATGTTGATTTTTGGCAT+GGG - chr4.3:36443461-36443480 MS.gene056633:intron 30.0%
!!! TGTTATGTTGATTTTTGGCA+TGG - chr4.3:36443460-36443479 MS.gene056633:intron 30.0%
AAAGAGAGAGGATCCTTTAA+GGG + chr4.3:36443546-36443565 None:intergenic 35.0%
AATGTTTGAGAGGAGCAAAA+TGG - chr4.3:36443764-36443783 MS.gene056633:CDS 35.0%
ACTTAGTAAATCCAATCCTG+TGG + chr4.3:36443799-36443818 None:intergenic 35.0%
CGATGAACTGAAAACAAAAG+TGG - chr4.3:36443710-36443729 MS.gene056633:CDS 35.0%
CGTTAAGATAATTGAACAGC+TGG + chr4.3:36443631-36443650 None:intergenic 35.0%
CTTGCTTATGCAGATTATGA+AGG + chr4.3:36443863-36443882 None:intergenic 35.0%
TATTGGACAAGTTGTGTCAA+AGG - chr4.3:36443299-36443318 MS.gene056633:CDS 35.0%
! GTATATATCGTCTCCTTTTC+AGG - chr4.3:36443656-36443675 MS.gene056633:intron 35.0%
!!! ATTTTTGGCATGGGCTTATA+TGG - chr4.3:36443470-36443489 MS.gene056633:CDS 35.0%
AAAATGGTTGTCATTGCCAC+AGG - chr4.3:36443780-36443799 MS.gene056633:intron 40.0%
CAAAGAGAGAGGATCCTTTA+AGG + chr4.3:36443547-36443566 None:intergenic 40.0%
CTTAAAGGATCCTCTCTCTT+TGG - chr4.3:36443545-36443564 MS.gene056633:CDS 40.0%
! CTGCTTCTAGTTGAAGCAAT+TGG - chr4.3:36443344-36443363 MS.gene056633:CDS 40.0%
!! CTTTGGAATGTTCGCTTTGA+AGG - chr4.3:36443562-36443581 MS.gene056633:CDS 40.0%
!! GAGCTGATTTTCATCCACTT+AGG + chr4.3:36443687-36443706 None:intergenic 40.0%
ACAGAAGGTTGCACATCATG+AGG + chr4.3:36443515-36443534 None:intergenic 45.0%
AGCGAACATTCCAAAGAGAG+AGG + chr4.3:36443558-36443577 None:intergenic 45.0%
AGGAGTTCACACTGGAAAGA+TGG - chr4.3:36443319-36443338 MS.gene056633:CDS 45.0%
GCTGCAGATGTTTATCTTGC+TGG - chr4.3:36443434-36443453 MS.gene056633:intron 45.0%
TGTGTCAAAGGAGTTCACAC+TGG - chr4.3:36443311-36443330 MS.gene056633:CDS 45.0%
!! TATCGTCTCCTTTTCAGGAG+AGG - chr4.3:36443661-36443680 MS.gene056633:intron 45.0%
CACTTAGGCCTCTCCTGAAA+AGG + chr4.3:36443672-36443691 None:intergenic 50.0%
GGTTGTCATTGCCACAGGAT+TGG - chr4.3:36443785-36443804 MS.gene056633:intron 50.0%
! GAGAGGATCCTTTAAGGGCA+CGG + chr4.3:36443541-36443560 None:intergenic 50.0%
!! CTTTTCAGGAGAGGCCTAAG+TGG - chr4.3:36443670-36443689 MS.gene056633:intron 50.0%
TCTGTCGACCGTGCCCTTAA+AGG - chr4.3:36443530-36443549 MS.gene056633:CDS 55.0%
! TTAAGGGCACGGTCGACAGA+AGG + chr4.3:36443530-36443549 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 36443162 36443887 36443162 ID=MS.gene056633
chr4.3 mRNA 36443162 36443887 36443162 ID=MS.gene056633.t1;Parent=MS.gene056633
chr4.3 exon 36443885 36443887 36443885 ID=MS.gene056633.t1.exon1;Parent=MS.gene056633.t1
chr4.3 CDS 36443885 36443887 36443885 ID=cds.MS.gene056633.t1;Parent=MS.gene056633.t1
chr4.3 exon 36443684 36443783 36443684 ID=MS.gene056633.t1.exon2;Parent=MS.gene056633.t1
chr4.3 CDS 36443684 36443783 36443684 ID=cds.MS.gene056633.t1;Parent=MS.gene056633.t1
chr4.3 exon 36443466 36443608 36443466 ID=MS.gene056633.t1.exon3;Parent=MS.gene056633.t1
chr4.3 CDS 36443466 36443608 36443466 ID=cds.MS.gene056633.t1;Parent=MS.gene056633.t1
chr4.3 exon 36443162 36443371 36443162 ID=MS.gene056633.t1.exon4;Parent=MS.gene056633.t1
chr4.3 CDS 36443162 36443371 36443162 ID=cds.MS.gene056633.t1;Parent=MS.gene056633.t1
Gene Sequence

>MS.gene056633

ATGGTAAGTAGTAGTAAAACAATATAGCTATTGTCATATTAATAAACTTATTCATGTCTTAGCCTTCATATATTTGACTGGATATATATTCTCGATCATTTTAGGGCTGTGTTTATATTGTTGAAGCATACAAAGTCTATTGGACAAGTTGTGTCAAAGGAGTTCACACTGGAAAGATGGTTCTGCTTCTAGTTGAAGCAATTGGTAAATTTATCATTTTTTTATACATACTCTGACCTTTCTTGAATTCAATAAAATGGACGAATCTTGCTGCTGCAGATGTTTATCTTGCTGGTACTGTTATGTTGATTTTTGGCATGGGCTTATATGGATTGTTCATCAGCAACACACCTCATGATGTGCAACCTTCTGTCGACCGTGCCCTTAAAGGATCCTCTCTCTTTGGAATGTTCGCTTTGAAGGTAATCATTATCGAACTATTTATAGATATTTAATTTTTAACAATCCAGCTGTTCAATTATCTTAACGAACATGTATATATCGTCTCCTTTTCAGGAGAGGCCTAAGTGGATGAAAATCAGCTCACTCGATGAACTGAAAACAAAAGTGGGGCATGTTATTGTAATGATTCTTCTTGTAAAAATGTTTGAGAGGAGCAAAATGGTTGTCATTGCCACAGGATTGGATTTACTAAGTTATTCAGTTTGTATTTTCTTATCATCTGCATCTTTGTATATCCTTCATAATCTGCATAAGCAAGATTAA

Protein sequence

>MS.gene056633.t1

MGCVYIVEAYKVYWTSCVKGVHTGKMVLLLVEAIDVYLAGTVMLIFGMGLYGLFISNTPHDVQPSVDRALKGSSLFGMFALKERPKWMKISSLDELKTKVGHVIVMILLVKMFERSKMVVIATGLDLLSYSVCIFLSSASLYILHNLHKQD