AlfalfaGEDB | MS.gene07383

M. sativa cultivar XinJiangDaYe / MS.gene07383

KEGG: -
GO: -
PFAM: PF04852.15

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene07383.t1	XP_003590857.2	96	201	3	2	1	200	1	197	5.50E-71	277.3

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene07383.t1	Q9LW68	87.6	129	16	0	51	179	43	171	6.1e-63	241.9

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene07383.t1	G7I5I7	96.0	201	3	2	1	200	1	197	4.0e-71	277.3

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene051719	MS.gene07383	0.823963	1.04E-53	-1.69E-46
MS.gene051720	MS.gene07383	0.818349	2.05E-52	-1.69E-46
MS.gene054791	MS.gene07383	0.801288	9.67E-49	-1.69E-46
MS.gene060519	MS.gene07383	0.818809	1.61E-52	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene07383.t1	MTR_1g075990	97.512	201	0	2	1	200	1	197	4.87e-142	394
MS.gene07383.t1	MTR_1g442860	72.487	189	43	2	1	180	1	189	4.70e-91	266
MS.gene07383.t1	MTR_4g094428	81.119	143	22	1	44	186	39	176	3.26e-86	254
MS.gene07383.t1	MTR_5g072510	78.571	140	30	0	50	189	31	170	4.04e-84	248
MS.gene07383.t1	MTR_7g097030	93.333	135	9	0	52	186	60	194	1.05e-82	244
MS.gene07383.t1	MTR_1g069825	94.656	131	7	0	56	186	52	182	1.27e-81	242
MS.gene07383.t1	MTR_2g016110	71.622	148	42	0	43	190	22	169	1.74e-74	223
MS.gene07383.t1	MTR_3g031830	80.597	134	26	0	56	189	42	175	3.17e-74	223
MS.gene07383.t1	MTR_2g092950	68.212	151	42	1	29	179	2	146	1.23e-73	219
MS.gene07383.t1	MTR_4g079930	68.707	147	41	1	33	179	11	152	2.43e-73	219
MS.gene07383.t1	MTR_2g076400	75.182	137	34	0	54	190	31	167	9.85e-73	218
MS.gene07383.t1	MTR_7g115700	65.839	161	48	3	28	187	4	158	1.26e-71	215
MS.gene07383.t1	MTR_1g080210	83.200	125	21	0	56	180	40	164	1.10e-68	207
MS.gene07383.t1	MTR_8g086350	73.016	126	34	0	54	179	29	154	1.69e-57	179
MS.gene07383.t1	MTR_1g114020	74.510	51	13	0	49	99	64	114	3.39e-21	86.3

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene07383.t1	AT2G31160	81.595	163	20	1	35	187	23	185	4.27e-84	248
MS.gene07383.t1	AT1G07090	78.723	141	27	1	47	187	25	162	1.55e-83	246
MS.gene07383.t1	AT3G23290	78.235	170	29	1	19	180	3	172	7.29e-82	241
MS.gene07383.t1	AT5G58500	76.596	141	32	1	55	194	18	158	4.48e-80	236
MS.gene07383.t1	AT5G28490	81.379	145	23	1	41	185	14	154	9.95e-74	221
MS.gene07383.t1	AT3G04510	82.308	130	23	0	56	185	33	162	1.05e-70	214
MS.gene07383.t1	AT2G42610	71.233	146	39	1	42	187	14	156	1.84e-70	212
MS.gene07383.t1	AT2G42610	71.233	146	39	1	42	187	14	156	1.84e-70	212
MS.gene07383.t1	AT1G78815	70.079	127	38	0	53	179	37	163	5.44e-66	201
MS.gene07383.t1	AT4G18610	69.841	126	37	1	55	179	36	161	4.20e-63	194
MS.gene07383.t1	AT1G16910	65.909	132	45	0	53	184	20	151	2.04e-62	191

Find 33 sgRNAs with CRISPR-Local

Find 41 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GGACAAGGTGCAGGTGGATT+TGG	0.285867	1.2:-64520352	None:intergenic
CTTAGAGTGTTTGTCGTTGC+TGG	0.294103	1.2:-64520151	None:intergenic
AGAAGAGACTGGAACACTTT+TGG	0.322091	1.2:+64520193	MS.gene07383:CDS
GACTCTTTCTTCGTGAAGTT+AGG	0.348358	1.2:+64520491	MS.gene07383:CDS
TTAAGGTATCTTGATCAGTT+TGG	0.366705	1.2:+64520286	MS.gene07383:CDS
TTGTCCTCTCAGACAAGCTT+GGG	0.367576	1.2:+64520375	MS.gene07383:CDS
GTGGTGGTCTATGGTTTCTT+AGG	0.368488	1.2:-64520222	None:intergenic
AAACCTGAAGATAACCCTTT+TGG	0.390636	1.2:+64520454	MS.gene07383:CDS
GGTGGTGGAGGTTGAATTTG+AGG	0.396468	1.2:-64520568	None:intergenic
ACTTAATTAGTTGCATTTGA+TGG	0.399489	1.2:-64520592	None:intergenic
GCACCAAAAGGGTTATCTTC+AGG	0.403413	1.2:-64520457	None:intergenic
ACTCTTTCTTCGTGAAGTTA+GGG	0.427767	1.2:+64520492	MS.gene07383:CDS
GTCTTCTGAGGTTGTTGTGT+TGG	0.429348	1.2:-64520080	None:intergenic
CTTGTCCTCTCAGACAAGCT+TGG	0.437451	1.2:+64520374	MS.gene07383:CDS
GCATTTGATGGTGGTGGTGG+AGG	0.439596	1.2:-64520580	None:intergenic
GTTGCATTTGATGGTGGTGG+TGG	0.444483	1.2:-64520583	None:intergenic
GGAAGTCTTGATGCATTGAT+TGG	0.461550	1.2:+64520397	MS.gene07383:CDS
CTAACAGCACGAGCACCAAA+AGG	0.492363	1.2:-64520469	None:intergenic
AGAGTGAAAGTGGTGGTCTA+TGG	0.493036	1.2:-64520231	None:intergenic
TTAGTTGCATTTGATGGTGG+TGG	0.493298	1.2:-64520586	None:intergenic
CTTTCACTCTCACGTTGTAG+TGG	0.531680	1.2:+64520244	MS.gene07383:CDS
AGAGGACAAGGACAAGGTGC+AGG	0.565916	1.2:-64520361	None:intergenic
CAAGCTTGTCTGAGAGGACA+AGG	0.577230	1.2:-64520373	None:intergenic
CTACAACGTGAGAGTGAAAG+TGG	0.580478	1.2:-64520241	None:intergenic
CTTCCCCAAGCTTGTCTGAG+AGG	0.586547	1.2:-64520379	None:intergenic
TAACAGCACGAGCACCAAAA+GGG	0.587993	1.2:-64520468	None:intergenic
GGACAAGGACAAGGTGCAGG+TGG	0.600667	1.2:-64520358	None:intergenic
GACTCTCAATCTAAAGCTAG+AGG	0.600860	1.2:+64520514	MS.gene07383:CDS
TAATTAGTTGCATTTGATGG+TGG	0.602526	1.2:-64520589	None:intergenic
AGAGTGTTTGTCGTTGCTGG+TGG	0.637477	1.2:-64520148	None:intergenic
TGTCCTCTCAGACAAGCTTG+GGG	0.658773	1.2:+64520376	MS.gene07383:CDS
TGTCTGAGAGGACAAGGACA+AGG	0.664143	1.2:-64520367	None:intergenic
CAACGTGAGAGTGAAAGTGG+TGG	0.746060	1.2:-64520238	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	CACATGTTCTTGAATTTTTA+AGG	+	chr1.2:64520269-64520288	MS.gene07383:CDS	25.0%
	AGAATCAAAAAAGAAGAGAC+TGG	+	chr1.2:64520182-64520201	MS.gene07383:CDS	30.0%
	TAATTAGTTGCATTTGATGG+TGG	-	chr1.2:64520592-64520611	None:intergenic	30.0%
	TGATCAGTTTGGAAAAACTA+AGG	+	chr1.2:64520297-64520316	MS.gene07383:CDS	30.0%
!	TTAAGGTATCTTGATCAGTT+TGG	+	chr1.2:64520286-64520305	MS.gene07383:CDS	30.0%
!!!	TCACAGATTTGTCCTTTTTT+TGG	+	chr1.2:64520325-64520344	MS.gene07383:CDS	30.0%
	ACTCTTTCTTCGTGAAGTTA+GGG	+	chr1.2:64520492-64520511	MS.gene07383:CDS	35.0%
	GGATTTGGATGACCAAAAAA+AGG	-	chr1.2:64520340-64520359	None:intergenic	35.0%
!	AAACCTGAAGATAACCCTTT+TGG	+	chr1.2:64520454-64520473	MS.gene07383:CDS	35.0%
!!	GATGTTTTTGTTGTCTTCTG+AGG	-	chr1.2:64520095-64520114	None:intergenic	35.0%
	GACTCTTTCTTCGTGAAGTT+AGG	+	chr1.2:64520491-64520510	MS.gene07383:CDS	40.0%
!	AGAAGAGACTGGAACACTTT+TGG	+	chr1.2:64520193-64520212	MS.gene07383:CDS	40.0%
!	GACTCTCAATCTAAAGCTAG+AGG	+	chr1.2:64520514-64520533	MS.gene07383:CDS	40.0%
!	GGAAGTCTTGATGCATTGAT+TGG	+	chr1.2:64520397-64520416	MS.gene07383:CDS	40.0%
!!	CTGCTTTTGAAGAGAATGGT+GGG	+	chr1.2:64520431-64520450	MS.gene07383:CDS	40.0%
!!	TTAGTTGCATTTGATGGTGG+TGG	-	chr1.2:64520589-64520608	None:intergenic	40.0%
!!!	AGAGCTGCTTTTGAAGAGAA+TGG	+	chr1.2:64520427-64520446	MS.gene07383:CDS	40.0%
	AGAGTGAAAGTGGTGGTCTA+TGG	-	chr1.2:64520234-64520253	None:intergenic	45.0%
	CTACAACGTGAGAGTGAAAG+TGG	-	chr1.2:64520244-64520263	None:intergenic	45.0%
	CTTTCACTCTCACGTTGTAG+TGG	+	chr1.2:64520244-64520263	MS.gene07383:CDS	45.0%
	GCACCAAAAGGGTTATCTTC+AGG	-	chr1.2:64520460-64520479	None:intergenic	45.0%
	GTGGTGGTCTATGGTTTCTT+AGG	-	chr1.2:64520225-64520244	None:intergenic	45.0%
	TAACAGCACGAGCACCAAAA+GGG	-	chr1.2:64520471-64520490	None:intergenic	45.0%
	TTGTCCTCTCAGACAAGCTT+GGG	+	chr1.2:64520375-64520394	MS.gene07383:CDS	45.0%
!	CTTAGAGTGTTTGTCGTTGC+TGG	-	chr1.2:64520154-64520173	None:intergenic	45.0%
!!	GTCTTCTGAGGTTGTTGTGT+TGG	-	chr1.2:64520083-64520102	None:intergenic	45.0%
!!!	GCTGCTTTTGAAGAGAATGG+TGG	+	chr1.2:64520430-64520449	MS.gene07383:CDS	45.0%
	CAACGTGAGAGTGAAAGTGG+TGG	-	chr1.2:64520241-64520260	None:intergenic	50.0%
	CAAGCTTGTCTGAGAGGACA+AGG	-	chr1.2:64520376-64520395	None:intergenic	50.0%
	CTAACAGCACGAGCACCAAA+AGG	-	chr1.2:64520472-64520491	None:intergenic	50.0%
	CTTGTCCTCTCAGACAAGCT+TGG	+	chr1.2:64520374-64520393	MS.gene07383:CDS	50.0%
	TGTCCTCTCAGACAAGCTTG+GGG	+	chr1.2:64520376-64520395	MS.gene07383:CDS	50.0%
	TGTCTGAGAGGACAAGGACA+AGG	-	chr1.2:64520370-64520389	None:intergenic	50.0%
!	AGAGTGTTTGTCGTTGCTGG+TGG	-	chr1.2:64520151-64520170	None:intergenic	50.0%
!!	GGTGGTGGAGGTTGAATTTG+AGG	-	chr1.2:64520571-64520590	None:intergenic	50.0%
!!	GTTGCATTTGATGGTGGTGG+TGG	-	chr1.2:64520586-64520605	None:intergenic	50.0%
	AGAGGACAAGGACAAGGTGC+AGG	-	chr1.2:64520364-64520383	None:intergenic	55.0%
	CTTCCCCAAGCTTGTCTGAG+AGG	-	chr1.2:64520382-64520401	None:intergenic	55.0%
!	GGACAAGGTGCAGGTGGATT+TGG	-	chr1.2:64520355-64520374	None:intergenic	55.0%
!!	GCATTTGATGGTGGTGGTGG+AGG	-	chr1.2:64520583-64520602	None:intergenic	55.0%
	GGACAAGGACAAGGTGCAGG+TGG	-	chr1.2:64520361-64520380	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.2	gene	64520010	64520612	64520010	ID=MS.gene07383
chr1.2	mRNA	64520010	64520612	64520010	ID=MS.gene07383.t1;Parent=MS.gene07383
chr1.2	exon	64520010	64520612	64520010	ID=MS.gene07383.t1.exon1;Parent=MS.gene07383.t1
chr1.2	CDS	64520010	64520612	64520010	ID=cds.MS.gene07383.t1;Parent=MS.gene07383.t1

Gene Sequence

>MS.gene07383

ATGAATTCATTTGAAGAATTCAACTCATCATCATCAACAAACACACAAACAAAAAACATCATCAACTTCACCAACACAACAACCTCAGAAGACAACAAAAACATCTCAAATTTCACATCTTCTTCTTCTTCAGCAGTACCACCAGCAACGACAAACACTCTAAGTCGCTATGAGAATCAAAAAAGAAGAGACTGGAACACTTTTGGTCAATACCTAAGAAACCATAGACCACCACTTTCACTCTCACGTTGTAGTGGAGCACATGTTCTTGAATTTTTAAGGTATCTTGATCAGTTTGGAAAAACTAAGGTTCACTCACAGATTTGTCCTTTTTTTGGTCATCCAAATCCACCTGCACCTTGTCCTTGTCCTCTCAGACAAGCTTGGGGAAGTCTTGATGCATTGATTGGAAGACTAAGAGCTGCTTTTGAAGAGAATGGTGGGAAACCTGAAGATAACCCTTTTGGTGCTCGTGCTGTTAGACTCTTTCTTCGTGAAGTTAGGGACTCTCAATCTAAAGCTAGAGGTATTAGCTATGAGAAAAAGAAGCGTAAAAGACCTCAAATTCAACCTCCACCACCACCATCAAATGCAACTAATTAA

Protein sequence

>MS.gene07383.t1

MNSFEEFNSSSSTNTQTKNIINFTNTTTSEDNKNISNFTSSSSSAVPPATTNTLSRYENQKRRDWNTFGQYLRNHRPPLSLSRCSGAHVLEFLRYLDQFGKTKVHSQICPFFGHPNPPAPCPCPLRQAWGSLDALIGRLRAAFEENGGKPEDNPFGARAVRLFLREVRDSQSKARGISYEKKKRKRPQIQPPPPPSNATN