AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene07386

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene07386.t1 MTR_1g076130 99.699 332 1 0 1 332 1 332 0.0 681
MS.gene07386.t1 MTR_1g043040 93.675 332 21 0 1 332 1 332 0.0 623
MS.gene07386.t1 MTR_8g005980 84.498 329 51 0 3 331 85 413 0.0 559
MS.gene07386.t1 MTR_1g090730 39.818 329 191 4 3 328 92 416 5.91e-74 234
MS.gene07386.t1 MTR_1g090730 41.852 270 153 1 3 272 92 357 1.85e-62 204
MS.gene07386.t1 MTR_7g037650 73.913 46 12 0 15 60 24 69 7.01e-14 72.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene07386.t1 AT1G04410 90.060 332 33 0 1 332 1 332 0.0 605
MS.gene07386.t1 AT5G43330 89.458 332 35 0 1 332 1 332 0.0 597
MS.gene07386.t1 AT5G56720 72.590 332 91 0 1 332 40 371 0.0 510
MS.gene07386.t1 AT5G58330 41.641 329 185 4 3 328 96 420 5.21e-79 248
MS.gene07386.t1 AT5G58330 41.641 329 185 4 3 328 97 421 5.83e-79 248
MS.gene07386.t1 AT5G58330 41.587 315 177 4 17 328 2 312 5.01e-76 237

Find 92 sgRNAs with CRISPR-Local

Find 183 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTGCATTCCCTCTTCTTAA+AGG 0.234412 1.2:-64472821 MS.gene07386:intron
TGGAGGATCACGGGCTGGTC+AGG 0.252087 1.2:+64472905 None:intergenic
ATCACTGAACGGTGTTAAAA+TGG 0.283888 1.2:-64472855 MS.gene07386:CDS
TGACAAGATTGGACCATAAC+AGG 0.286946 1.2:-64471727 MS.gene07386:CDS
CACATTAGAGATTGGGTTCT+AGG 0.308508 1.2:-64471319 MS.gene07386:CDS
CATTTCTTGTTTGACAAGAT+TGG 0.308718 1.2:-64471738 MS.gene07386:CDS
ATCAAGGCATTGGTGTTTGC+CGG 0.326996 1.2:+64471797 None:intergenic
TGCAATATCATCAGGGAACC+TGG 0.363492 1.2:-64471205 MS.gene07386:intron
TGGAGTCAATATTGCTGTTA+TGG 0.370670 1.2:-64472629 MS.gene07386:CDS
CTCTTGTCCCTATGATTGCC+AGG 0.371373 1.2:-64472943 MS.gene07386:CDS
TATTTGCCATTCGCCATTGC+GGG 0.380422 1.2:+64471109 None:intergenic
ACAGTTGCGTGGTTGACATC+AGG 0.383245 1.2:+64471614 None:intergenic
TGATTGCAGCACCCCGTTGT+TGG 0.384508 1.2:+64471395 None:intergenic
TTTGACCCAGAGCCCTGTTA+TGG 0.392891 1.2:+64471714 None:intergenic
TCGCCATTGCGGGTTGTGAC+AGG 0.397845 1.2:+64471119 None:intergenic
ATCACTCCCCTGGCAATCAT+AGG 0.399565 1.2:+64472935 None:intergenic
GCTTTCTGAGGAGAAGGCTT+TGG 0.399799 1.2:-64470847 MS.gene07386:CDS
CTTGTGACCACATTAGAGAT+TGG 0.400822 1.2:-64471327 MS.gene07386:CDS
CATAGCTACCGTCCAACAAC+GGG 0.401578 1.2:-64471407 MS.gene07386:CDS
CACCAATGCCTTGATCTTGA+AGG 0.415103 1.2:-64471789 MS.gene07386:CDS
TTTAACACCGTTCAGTGATT+CGG 0.419237 1.2:+64472859 None:intergenic
GGCATTGGTGTTTGCCGGGT+TGG 0.424208 1.2:+64471802 None:intergenic
CCTGTTCGTGTTCTTGTCAC+TGG 0.433814 1.2:-64473653 MS.gene07386:CDS
CCAGTGACAAGAACACGAAC+AGG 0.438629 1.2:+64473653 None:intergenic
TTGTGACCACATTAGAGATT+GGG 0.444343 1.2:-64471326 MS.gene07386:CDS
CCGTTCAGTGATTCGGCTGC+TGG 0.450461 1.2:+64472866 None:intergenic
TTTCTCAAGAGCAGAAGCTT+GGG 0.453532 1.2:+64472534 None:intergenic
GTTTCTCAAGAGCAGAAGCT+TGG 0.456679 1.2:+64472533 None:intergenic
ATTGGACCATAACAGGGCTC+TGG 0.463189 1.2:-64471720 MS.gene07386:CDS
TTCAGTGATTCGGCTGCTGG+TGG 0.473142 1.2:+64472869 None:intergenic
AATATTGCTGTTATGGTTGG+TGG 0.480289 1.2:-64472622 MS.gene07386:CDS
GTCAATATTGCTGTTATGGT+TGG 0.483183 1.2:-64472625 MS.gene07386:CDS
GGAGGATCACGGGCTGGTCA+GGG 0.487190 1.2:+64472906 None:intergenic
AACTCGTGGCTGATGATGCC+TGG 0.490237 1.2:-64471559 MS.gene07386:intron
GTTCTTGTCACTGGTGCTGC+AGG 0.494198 1.2:-64473644 MS.gene07386:intron
GGCGAATGGCAAATAGTTCA+AGG 0.495465 1.2:-64471101 MS.gene07386:intron
TTCCTTCAAGATCAAGGCAT+TGG 0.495936 1.2:+64471787 None:intergenic
TGCTACAACTGATGTGGTTG+AGG 0.500221 1.2:-64472659 MS.gene07386:CDS
CGCCATTGCGGGTTGTGACA+GGG 0.503139 1.2:+64471120 None:intergenic
AGGGCCGAGCATCACTCCCC+TGG 0.504498 1.2:+64472925 None:intergenic
GGTTCTCGTTGTTGCCAACC+CGG 0.510041 1.2:-64471816 MS.gene07386:CDS
AGAAGAGCTTTCTGAGGAGA+AGG 0.511032 1.2:-64470853 MS.gene07386:CDS
TCAAGGCATTGGTGTTTGCC+GGG 0.513479 1.2:+64471798 None:intergenic
AGCGAATTCCTTCAAGATCA+AGG 0.519920 1.2:+64471781 None:intergenic
TCAGGGAACCTGGGTATCGA+TGG 0.524300 1.2:-64471195 MS.gene07386:intron
GGATCTTACAATGTACCTGC+TGG 0.524735 1.2:-64471158 MS.gene07386:CDS
GGTGGATTCCCTAGAAAAGA+AGG 0.528773 1.2:-64472604 MS.gene07386:CDS
CGGTGTTAAAATGGAGTTGG+TGG 0.529018 1.2:-64472846 MS.gene07386:CDS
TCAAGCATGTGGAGGATCAC+GGG 0.532929 1.2:+64472896 None:intergenic
ATTCCCTAGAAAAGAAGGTA+TGG 0.539427 1.2:-64472599 MS.gene07386:CDS
TCGATGGGAGTTTATTCTGA+TGG 0.539461 1.2:-64471179 MS.gene07386:CDS
GTCAAGCATGTGGAGGATCA+CGG 0.540541 1.2:+64472895 None:intergenic
AATGAATAGATTAGTCCAGC+AGG 0.546223 1.2:+64471143 None:intergenic
CAGTTGCGTGGTTGACATCA+GGG 0.549966 1.2:+64471615 None:intergenic
AACTCAAACCTTTAAGAAGA+GGG 0.552886 1.2:+64472813 None:intergenic
TCACTCCCCTGGCAATCATA+GGG 0.553376 1.2:+64472936 None:intergenic
TTGGACCATAACAGGGCTCT+GGG 0.556375 1.2:-64471719 MS.gene07386:CDS
TGCAGCACCCCGTTGTTGGA+CGG 0.557691 1.2:+64471399 None:intergenic
CTATTTGCCATTCGCCATTG+CGG 0.559494 1.2:+64471108 None:intergenic
TCACAACCCGCAATGGCGAA+TGG 0.563222 1.2:-64471115 MS.gene07386:CDS
GACAGCAGAAGAGCTTTCTG+AGG 0.563983 1.2:-64470859 MS.gene07386:CDS
TTCCCTGTCACAACCCGCAA+TGG 0.564846 1.2:-64471122 MS.gene07386:CDS
ATTGCCAGGGGAGTGATGCT+CGG 0.570606 1.2:-64472929 MS.gene07386:CDS
CATCATCAGCCACGAGTTCA+CGG 0.570980 1.2:+64471564 None:intergenic
AAACTCAAACCTTTAAGAAG+AGG 0.571371 1.2:+64472812 None:intergenic
TCTTGTCCCTATGATTGCCA+GGG 0.574263 1.2:-64472942 MS.gene07386:CDS
GCATGTGGAGGATCACGGGC+TGG 0.580601 1.2:+64472900 None:intergenic
TCAGTGATTCGGCTGCTGGT+GGG 0.585206 1.2:+64472870 None:intergenic
GATGTGGTTGAGGGATGCAC+TGG 0.588132 1.2:-64472649 MS.gene07386:CDS
CAGGGAACCTGGGTATCGAT+GGG 0.588520 1.2:-64471194 MS.gene07386:CDS
GTGGTCACAAGCAGCGCTAG+CGG 0.592624 1.2:+64471339 None:intergenic
GCTACAACTGATGTGGTTGA+GGG 0.594490 1.2:-64472658 MS.gene07386:CDS
GACAAGATTGGACCATAACA+GGG 0.599626 1.2:-64471726 MS.gene07386:CDS
ATAAACTCCCATCGATACCC+AGG 0.604419 1.2:+64471187 None:intergenic
CTGGTGGGATGTCAAGCATG+TGG 0.608538 1.2:+64472885 None:intergenic
CAGGGTACTGACTTGATGAA+TGG 0.610269 1.2:+64471633 None:intergenic
CTAGAACCCAATCTCTAATG+TGG 0.612432 1.2:+64471320 None:intergenic
TCATAGCTACCGTCCAACAA+CGG 0.613333 1.2:-64471408 MS.gene07386:CDS
TGTTGTTGCTACAACTGATG+TGG 0.615089 1.2:-64472665 MS.gene07386:CDS
TCAGCCACGAGTTCACGGAC+AGG 0.623183 1.2:+64471569 None:intergenic
ACATGCTGCTGCAAACTGCA+AGG 0.624025 1.2:-64472512 MS.gene07386:intron
GCAATATCATCAGGGAACCT+GGG 0.624982 1.2:-64471204 MS.gene07386:intron
GAACGGTGTTAAAATGGAGT+TGG 0.636523 1.2:-64472849 MS.gene07386:CDS
AAAGCCTGTCCGTGAACTCG+TGG 0.642217 1.2:-64471573 MS.gene07386:CDS
TTGGGTTCTAGGAACTCCTG+AGG 0.644314 1.2:-64471308 MS.gene07386:intron
GGAAACAAGCTGTCTAAGAG+AGG 0.644922 1.2:+64470814 None:intergenic
CTTGTCCCTATGATTGCCAG+GGG 0.651791 1.2:-64472941 MS.gene07386:CDS
CCAGCAGCCGAATCACTGAA+CGG 0.657663 1.2:-64472866 MS.gene07386:CDS
CTAGAAAAGAAGGTATGGAG+AGG 0.663254 1.2:-64472594 MS.gene07386:CDS
GTGGGATGTCAAGCATGTGG+AGG 0.682561 1.2:+64472888 None:intergenic
CAGTGACAAGAACACGAACA+GGG 0.694789 1.2:+64473654 None:intergenic
ATAGCTACCGTCCAACAACG+GGG 0.807747 1.2:-64471406 MS.gene07386:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTAATTCAAATTCTTTATA+TGG - chr1.2:64473228-64473247 MS.gene07386:intron 10.0%
!! AGAAAATTGCATAATTATAA+GGG + chr1.2:64471170-64471189 None:intergenic 15.0%
!! ATGTGAAAAATGTTATAATT+TGG - chr1.2:64472831-64472850 MS.gene07386:CDS 15.0%
!! TGTGAAAAATGTTATAATTT+GGG - chr1.2:64472832-64472851 MS.gene07386:CDS 15.0%
!!! TTAAACATTTTAACCATATT+TGG + chr1.2:64471436-64471455 None:intergenic 15.0%
!!! TTGTTTTATATAGCAAATAA+AGG - chr1.2:64473517-64473536 MS.gene07386:intron 15.0%
!! ACAACTTAATTAAATTCGTA+TGG + chr1.2:64472365-64472384 None:intergenic 20.0%
!! ATTTCAACATAAAATCTTTG+AGG - chr1.2:64471470-64471489 MS.gene07386:intron 20.0%
!! ATTTCTATAAACTCTCAAAT+AGG + chr1.2:64471221-64471240 None:intergenic 20.0%
!! CAGAAAATTGCATAATTATA+AGG + chr1.2:64471171-64471190 None:intergenic 20.0%
!! GTGAAAAATGTTATAATTTG+GGG - chr1.2:64472833-64472852 MS.gene07386:CDS 20.0%
!! GTTTCCAAAAATATGTAAAA+AGG + chr1.2:64472600-64472619 None:intergenic 20.0%
!! TTGTGAAAATTATTTGTTTC+AGG - chr1.2:64471804-64471823 MS.gene07386:CDS 20.0%
!! TTTAGAAATAGAACTATGTT+TGG - chr1.2:64470998-64471017 MS.gene07386:intron 20.0%
!! TTTCTATAAACTCTCAAATA+GGG + chr1.2:64471220-64471239 None:intergenic 20.0%
!!! AAAAATGTTTTGCCAATTTT+AGG - chr1.2:64471512-64471531 MS.gene07386:intron 20.0%
!!! AGATTTTGATAAGTTATTAC+TGG - chr1.2:64473416-64473435 MS.gene07386:intron 20.0%
!!! ATTTGTCTAATTTTTTAACC+AGG - chr1.2:64473571-64473590 MS.gene07386:intron 20.0%
!!! CATATTTTTGACATAAAATG+CGG + chr1.2:64472066-64472085 None:intergenic 20.0%
!!! TACGAATTTAATTAAGTTGT+TGG - chr1.2:64472365-64472384 MS.gene07386:intron 20.0%
!!! TAGTCCTTTTTACATATTTT+TGG - chr1.2:64472593-64472612 MS.gene07386:CDS 20.0%
!!! TATGTCTTTTGTTTTGTTTA+AGG - chr1.2:64470904-64470923 MS.gene07386:intron 20.0%
! AAATGCTTTGCAAGATTAAA+AGG - chr1.2:64471779-64471798 MS.gene07386:CDS 25.0%
! AACTAAAATAAATTCAGTGC+AGG + chr1.2:64472458-64472477 None:intergenic 25.0%
! ACAGAGTTTAAAGAAATGTA+AGG + chr1.2:64472645-64472664 None:intergenic 25.0%
! AGGTAACCATAATAAATTCT+GGG - chr1.2:64470868-64470887 MS.gene07386:CDS 25.0%
! ATACTTCAGTAAATGCAAAA+TGG + chr1.2:64473028-64473047 None:intergenic 25.0%
! CAAAAATATGTTCCATTCTA+TGG - chr1.2:64472076-64472095 MS.gene07386:intron 25.0%
! GCCTTAATACAAATAAAAGA+CGG + chr1.2:64473493-64473512 None:intergenic 25.0%
! TATCCAATTTGTCCTAAAAT+TGG + chr1.2:64471527-64471546 None:intergenic 25.0%
! TGAAAGAGATACATGAAAAA+GGG + chr1.2:64472112-64472131 None:intergenic 25.0%
! TGAAGTTGTTTATCCAAATA+TGG - chr1.2:64471420-64471439 MS.gene07386:CDS 25.0%
! TTTATTTCCATAATCTCTCT+AGG - chr1.2:64471272-64471291 MS.gene07386:intron 25.0%
!! AAACAAGAAATGTTTTTCTC+AGG + chr1.2:64472746-64472765 None:intergenic 25.0%
!! TAAATGTTACTTGAACTTCT+TGG - chr1.2:64471713-64471732 MS.gene07386:CDS 25.0%
!! TTTCAATTGATGAGTTTTCA+AGG - chr1.2:64473596-64473615 MS.gene07386:intron 25.0%
!! TTTTCATTGCAATATCATCA+GGG - chr1.2:64473280-64473299 MS.gene07386:intron 25.0%
!!! ATGTGTATTTTGGTCATATA+TGG - chr1.2:64472512-64472531 MS.gene07386:intron 25.0%
!!! TCCGTCTTTTATTTGTATTA+AGG - chr1.2:64473489-64473508 MS.gene07386:intron 25.0%
!!! TGAGTTTTCAAGGAAAAAAT+TGG - chr1.2:64473606-64473625 MS.gene07386:intron 25.0%
AAAGAGATACATGAAAAAGG+GGG + chr1.2:64472110-64472129 None:intergenic 30.0%
AAGTTATCCTAGAGAGATTA+TGG + chr1.2:64471282-64471301 None:intergenic 30.0%
ATATGGAATGACTTCAACTA+AGG - chr1.2:64473245-64473264 MS.gene07386:intron 30.0%
CAATCAAGAAACATGATACA+AGG + chr1.2:64470972-64470991 None:intergenic 30.0%
CAGGTAACCATAATAAATTC+TGG - chr1.2:64470867-64470886 MS.gene07386:CDS 30.0%
CTGAAAGAGATACATGAAAA+AGG + chr1.2:64472113-64472132 None:intergenic 30.0%
CTTGTATCATGTTTCTTGAT+TGG - chr1.2:64470970-64470989 MS.gene07386:intron 30.0%
GAAAGAGATACATGAAAAAG+GGG + chr1.2:64472111-64472130 None:intergenic 30.0%
GAGAAACCCAGAATTTATTA+TGG + chr1.2:64470877-64470896 None:intergenic 30.0%
TAGGGAAAATACTACAATTG+AGG + chr1.2:64471202-64471221 None:intergenic 30.0%
TCAAATAAGTCAATCCAAAC+AGG + chr1.2:64472137-64472156 None:intergenic 30.0%
! AAACTCAAACCTTTAAGAAG+AGG + chr1.2:64471683-64471702 None:intergenic 30.0%
! AACTCAAACCTTTAAGAAGA+GGG + chr1.2:64471682-64471701 None:intergenic 30.0%
! AGAAATGTTTTTCTCAGGAA+TGG + chr1.2:64472741-64472760 None:intergenic 30.0%
! CATTTCTTGTTTGACAAGAT+TGG - chr1.2:64472754-64472773 MS.gene07386:intron 30.0%
! CTTTTCATTGCAATATCATC+AGG - chr1.2:64473279-64473298 MS.gene07386:intron 30.0%
! TGATGTAATTTTCATCGTCA+AGG - chr1.2:64473048-64473067 MS.gene07386:intron 30.0%
! TTAGTTTAGTGCTGAAAAAG+TGG - chr1.2:64472472-64472491 MS.gene07386:intron 30.0%
! TTTTCATCGTCAAGGTTAAA+TGG - chr1.2:64473056-64473075 MS.gene07386:intron 30.0%
!!! TTGCCAATTTTAGGACAAAT+TGG - chr1.2:64471521-64471540 MS.gene07386:intron 30.0%
AACTCATCAATTGAAAGTCC+TGG + chr1.2:64473592-64473611 None:intergenic 35.0%
AGAAACAGATTTCACCAGAA+TGG + chr1.2:64472554-64472573 None:intergenic 35.0%
ATTAACTGCTTGCAACTACT+AGG + chr1.2:64471096-64471115 None:intergenic 35.0%
ATTCAGTGCAGGTAATGTAT+TGG + chr1.2:64472447-64472466 None:intergenic 35.0%
ATTCCCTAGAAAAGAAGGTA+TGG - chr1.2:64471893-64471912 MS.gene07386:intron 35.0%
CCAACTTCAAACCAGAAATA+TGG + chr1.2:64471743-64471762 None:intergenic 35.0%
CTTATTTCCATAAGCTCTAC+AGG - chr1.2:64472246-64472265 MS.gene07386:intron 35.0%
GCTTGAGCATTCAGAATTAA+AGG + chr1.2:64472010-64472029 None:intergenic 35.0%
TATTTGTCCATCAACATACC+AGG + chr1.2:64472954-64472973 None:intergenic 35.0%
TTGTGACCACATTAGAGATT+GGG - chr1.2:64473166-64473185 MS.gene07386:intron 35.0%
TTTAACACCGTTCAGTGATT+CGG + chr1.2:64471636-64471655 None:intergenic 35.0%
! AATATTGCTGTTATGGTTGG+TGG - chr1.2:64471870-64471889 MS.gene07386:intron 35.0%
! AATGAATAGATTAGTCCAGC+AGG + chr1.2:64473352-64473371 None:intergenic 35.0%
! ATCACTGAACGGTGTTAAAA+TGG - chr1.2:64471637-64471656 MS.gene07386:CDS 35.0%
! ATGTTTTTCTCAGGAATGGA+TGG + chr1.2:64472737-64472756 None:intergenic 35.0%
! GTCAATATTGCTGTTATGGT+TGG - chr1.2:64471867-64471886 MS.gene07386:intron 35.0%
! TCTCCATACCTTCTTTTCTA+GGG + chr1.2:64471899-64471918 None:intergenic 35.0%
! TGGAGTCAATATTGCTGTTA+TGG - chr1.2:64471863-64471882 MS.gene07386:intron 35.0%
!! CCATATTTCTGGTTTGAAGT+TGG - chr1.2:64471740-64471759 MS.gene07386:CDS 35.0%
!! TGGTTGGTTCATGTGTATTT+TGG - chr1.2:64472502-64472521 MS.gene07386:intron 35.0%
AAAAAGTGGTTCCATCTGGT+TGG - chr1.2:64472486-64472505 MS.gene07386:intron 40.0%
AAGCTATCCTGTAGAGCTTA+TGG + chr1.2:64472256-64472275 None:intergenic 40.0%
AAGTCCTTGTGATTCCATTC+TGG - chr1.2:64472537-64472556 MS.gene07386:CDS 40.0%
AGCGAATTCCTTCAAGATCA+AGG + chr1.2:64472714-64472733 None:intergenic 40.0%
ATACACATGAACCAACCAGA+TGG + chr1.2:64472500-64472519 None:intergenic 40.0%
CACATTAGAGATTGGGTTCT+AGG - chr1.2:64473173-64473192 MS.gene07386:intron 40.0%
CTAGAACCCAATCTCTAATG+TGG + chr1.2:64473175-64473194 None:intergenic 40.0%
CTTGTGACCACATTAGAGAT+TGG - chr1.2:64473165-64473184 MS.gene07386:intron 40.0%
GACAAGATTGGACCATAACA+GGG - chr1.2:64472766-64472785 MS.gene07386:intron 40.0%
GTATCTCTTTCAGACCTGTT+TGG - chr1.2:64472120-64472139 MS.gene07386:intron 40.0%
TAAACTCTGTATCACCATGC+AGG - chr1.2:64472655-64472674 MS.gene07386:CDS 40.0%
TCGATGGGAGTTTATTCTGA+TGG - chr1.2:64473313-64473332 MS.gene07386:intron 40.0%
TGACAAGATTGGACCATAAC+AGG - chr1.2:64472765-64472784 MS.gene07386:intron 40.0%
TGTTGTTGCTACAACTGATG+TGG - chr1.2:64471827-64471846 MS.gene07386:intron 40.0%
TTCACCAGAATGGAATCACA+AGG + chr1.2:64472544-64472563 None:intergenic 40.0%
TTCCTTCAAGATCAAGGCAT+TGG + chr1.2:64472708-64472727 None:intergenic 40.0%
TTCTTGGACAGCCATATTTC+TGG - chr1.2:64471729-64471748 MS.gene07386:CDS 40.0%
TTTCTCAAGAGCAGAAGCTT+GGG + chr1.2:64471961-64471980 None:intergenic 40.0%
! CTAGAAAAGAAGGTATGGAG+AGG - chr1.2:64471898-64471917 MS.gene07386:intron 40.0%
! CTCTCCATACCTTCTTTTCT+AGG + chr1.2:64471900-64471919 None:intergenic 40.0%
! GAACGGTGTTAAAATGGAGT+TGG - chr1.2:64471643-64471662 MS.gene07386:CDS 40.0%
! GGTTTGAAGTTGGATTGTGT+TGG - chr1.2:64471750-64471769 MS.gene07386:CDS 40.0%
!! AAGCGATTTTAACAGTTGCG+TGG + chr1.2:64472892-64472911 None:intergenic 40.0%
AAGCACTTGTGAGACTGCTT+GGG - chr1.2:64472177-64472196 MS.gene07386:intron 45.0%
AGCAAGACATAACCAACCTC+AGG + chr1.2:64473203-64473222 None:intergenic 45.0%
ATAAACTCCCATCGATACCC+AGG + chr1.2:64473308-64473327 None:intergenic 45.0%
ATACATGAAAAAGGGGGAGG+AGG + chr1.2:64472104-64472123 None:intergenic 45.0%
CACCAATGCCTTGATCTTGA+AGG - chr1.2:64472703-64472722 MS.gene07386:intron 45.0%
CAGTGACAAGAACACGAACA+GGG + chr1.2:64470841-64470860 None:intergenic 45.0%
CTATTTGCCATTCGCCATTG+CGG + chr1.2:64473387-64473406 None:intergenic 45.0%
GAGATACATGAAAAAGGGGG+AGG + chr1.2:64472107-64472126 None:intergenic 45.0%
GATGATGCCTGGTATGTTGA+TGG - chr1.2:64472944-64472963 MS.gene07386:CDS 45.0%
GCAATATCATCAGGGAACCT+GGG - chr1.2:64473288-64473307 MS.gene07386:intron 45.0%
GCTACAACTGATGTGGTTGA+GGG - chr1.2:64471834-64471853 MS.gene07386:intron 45.0%
GCTGAAAAAGTGGTTCCATC+TGG - chr1.2:64472482-64472501 MS.gene07386:intron 45.0%
GCTGCATTCCCTCTTCTTAA+AGG - chr1.2:64471671-64471690 MS.gene07386:CDS 45.0%
GGATCTTACAATGTACCTGC+TGG - chr1.2:64473334-64473353 MS.gene07386:intron 45.0%
GGCGAATGGCAAATAGTTCA+AGG - chr1.2:64473391-64473410 MS.gene07386:intron 45.0%
GTCTCACAAGTGCTTGCTTA+TGG + chr1.2:64472172-64472191 None:intergenic 45.0%
GTTTCTCAAGAGCAGAAGCT+TGG + chr1.2:64471962-64471981 None:intergenic 45.0%
TATTTGCCATTCGCCATTGC+GGG + chr1.2:64473386-64473405 None:intergenic 45.0%
TCATAGCTACCGTCCAACAA+CGG - chr1.2:64473084-64473103 MS.gene07386:intron 45.0%
TCTTGTCCCTATGATTGCCA+GGG - chr1.2:64471550-64471569 MS.gene07386:intron 45.0%
TGCAATATCATCAGGGAACC+TGG - chr1.2:64473287-64473306 MS.gene07386:intron 45.0%
TGCTACAACTGATGTGGTTG+AGG - chr1.2:64471833-64471852 MS.gene07386:intron 45.0%
! AGAAGAGCTTTCTGAGGAGA+AGG - chr1.2:64473639-64473658 MS.gene07386:intron 45.0%
! CGGTGTTAAAATGGAGTTGG+TGG - chr1.2:64471646-64471665 MS.gene07386:CDS 45.0%
! GGTGGATTCCCTAGAAAAGA+AGG - chr1.2:64471888-64471907 MS.gene07386:intron 45.0%
!! ATCAAGGCATTGGTGTTTGC+CGG + chr1.2:64472698-64472717 None:intergenic 45.0%
!! CAGGGTACTGACTTGATGAA+TGG + chr1.2:64472862-64472881 None:intergenic 45.0%
ACAGTTGCGTGGTTGACATC+AGG + chr1.2:64472881-64472900 None:intergenic 50.0%
ACATGCTGCTGCAAACTGCA+AGG - chr1.2:64471980-64471999 MS.gene07386:intron 50.0%
ATAGCTACCGTCCAACAACG+GGG - chr1.2:64473086-64473105 MS.gene07386:intron 50.0%
ATCACTCCCCTGGCAATCAT+AGG + chr1.2:64471560-64471579 None:intergenic 50.0%
ATTGGACCATAACAGGGCTC+TGG - chr1.2:64472772-64472791 MS.gene07386:intron 50.0%
CAAGCACTTGTGAGACTGCT+TGG - chr1.2:64472176-64472195 MS.gene07386:intron 50.0%
CAGTTGCGTGGTTGACATCA+GGG + chr1.2:64472880-64472899 None:intergenic 50.0%
CATAGCTACCGTCCAACAAC+GGG - chr1.2:64473085-64473104 MS.gene07386:intron 50.0%
CATCATCAGCCACGAGTTCA+CGG + chr1.2:64472931-64472950 None:intergenic 50.0%
CCAGTGACAAGAACACGAAC+AGG + chr1.2:64470842-64470861 None:intergenic 50.0%
CCTGTTCGTGTTCTTGTCAC+TGG - chr1.2:64470839-64470858 MS.gene07386:CDS 50.0%
CTCTTGTCCCTATGATTGCC+AGG - chr1.2:64471549-64471568 MS.gene07386:intron 50.0%
CTTGTCCCTATGATTGCCAG+GGG - chr1.2:64471551-64471570 MS.gene07386:intron 50.0%
GACAGCAGAAGAGCTTTCTG+AGG - chr1.2:64473633-64473652 MS.gene07386:intron 50.0%
GTCAAGCATGTGGAGGATCA+CGG + chr1.2:64471600-64471619 None:intergenic 50.0%
TCAAGCATGTGGAGGATCAC+GGG + chr1.2:64471599-64471618 None:intergenic 50.0%
TCACTCCCCTGGCAATCATA+GGG + chr1.2:64471559-64471578 None:intergenic 50.0%
TTGGACCATAACAGGGCTCT+GGG - chr1.2:64472773-64472792 MS.gene07386:intron 50.0%
TTTGACCCAGAGCCCTGTTA+TGG + chr1.2:64472781-64472800 None:intergenic 50.0%
! GCTTTCTGAGGAGAAGGCTT+TGG - chr1.2:64473645-64473664 MS.gene07386:CDS 50.0%
!! GTTCTAGGAACTCCTGAGGT+TGG - chr1.2:64473188-64473207 MS.gene07386:intron 50.0%
!! TCAAGGCATTGGTGTTTGCC+GGG + chr1.2:64472697-64472716 None:intergenic 50.0%
!! TTGGGTTCTAGGAACTCCTG+AGG - chr1.2:64473184-64473203 MS.gene07386:intron 50.0%
AAAGCCTGTCCGTGAACTCG+TGG - chr1.2:64472919-64472938 MS.gene07386:CDS 55.0%
AGACTGCTTGGGAGAGCTCA+TGG - chr1.2:64472188-64472207 MS.gene07386:intron 55.0%
ATTGCCAGGGGAGTGATGCT+CGG - chr1.2:64471563-64471582 MS.gene07386:CDS 55.0%
CCAGCAGCCGAATCACTGAA+CGG - chr1.2:64471626-64471645 MS.gene07386:CDS 55.0%
CTGGTGGGATGTCAAGCATG+TGG + chr1.2:64471610-64471629 None:intergenic 55.0%
GATGTGGTTGAGGGATGCAC+TGG - chr1.2:64471843-64471862 MS.gene07386:intron 55.0%
GGCAACAACGAGAACCTGCA+TGG + chr1.2:64472672-64472691 None:intergenic 55.0%
GGGGAGGAGGAACCATAGAA+TGG + chr1.2:64472091-64472110 None:intergenic 55.0%
GGTTCTCGTTGTTGCCAACC+CGG - chr1.2:64472676-64472695 MS.gene07386:intron 55.0%
GTGGGATGTCAAGCATGTGG+AGG + chr1.2:64471607-64471626 None:intergenic 55.0%
TCACAACCCGCAATGGCGAA+TGG - chr1.2:64473377-64473396 MS.gene07386:intron 55.0%
TGATTGCAGCACCCCGTTGT+TGG + chr1.2:64473100-64473119 None:intergenic 55.0%
TTCCCTGTCACAACCCGCAA+TGG - chr1.2:64473370-64473389 MS.gene07386:intron 55.0%
! AACTCGTGGCTGATGATGCC+TGG - chr1.2:64472933-64472952 MS.gene07386:CDS 55.0%
! TCAGTGATTCGGCTGCTGGT+GGG + chr1.2:64471625-64471644 None:intergenic 55.0%
! TTCAGTGATTCGGCTGCTGG+TGG + chr1.2:64471626-64471645 None:intergenic 55.0%
!! CAGGGAACCTGGGTATCGAT+GGG - chr1.2:64473298-64473317 MS.gene07386:intron 55.0%
!! GTTCTTGTCACTGGTGCTGC+AGG - chr1.2:64470848-64470867 MS.gene07386:CDS 55.0%
!! TCAGGGAACCTGGGTATCGA+TGG - chr1.2:64473297-64473316 MS.gene07386:intron 55.0%
GTGGTCACAAGCAGCGCTAG+CGG + chr1.2:64473156-64473175 None:intergenic 60.0%
TCAGCCACGAGTTCACGGAC+AGG + chr1.2:64472926-64472945 None:intergenic 60.0%
TGCAGCACCCCGTTGTTGGA+CGG + chr1.2:64473096-64473115 None:intergenic 60.0%
! CCGTTCAGTGATTCGGCTGC+TGG + chr1.2:64471629-64471648 None:intergenic 60.0%
! CGCCATTGCGGGTTGTGACA+GGG + chr1.2:64473375-64473394 None:intergenic 60.0%
! TCGCCATTGCGGGTTGTGAC+AGG + chr1.2:64473376-64473395 None:intergenic 60.0%
!! GGCATTGGTGTTTGCCGGGT+TGG + chr1.2:64472693-64472712 None:intergenic 60.0%
GCATGTGGAGGATCACGGGC+TGG + chr1.2:64471595-64471614 None:intergenic 65.0%
!! GGAGGATCACGGGCTGGTCA+GGG + chr1.2:64471589-64471608 None:intergenic 65.0%
!! TGGAGGATCACGGGCTGGTC+AGG + chr1.2:64471590-64471609 None:intergenic 65.0%
AGGGCCGAGCATCACTCCCC+TGG + chr1.2:64471570-64471589 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 64470827 64473687 64470827 ID=MS.gene07386
chr1.2 mRNA 64470827 64473687 64470827 ID=MS.gene07386.t1;Parent=MS.gene07386
chr1.2 exon 64473645 64473687 64473645 ID=MS.gene07386.t1.exon1;Parent=MS.gene07386.t1
chr1.2 CDS 64473645 64473687 64473645 ID=cds.MS.gene07386.t1;Parent=MS.gene07386.t1
chr1.2 exon 64472822 64472980 64472822 ID=MS.gene07386.t1.exon2;Parent=MS.gene07386.t1
chr1.2 CDS 64472822 64472980 64472822 ID=cds.MS.gene07386.t1;Parent=MS.gene07386.t1
chr1.2 exon 64472513 64472688 64472513 ID=MS.gene07386.t1.exon3;Parent=MS.gene07386.t1
chr1.2 CDS 64472513 64472688 64472513 ID=cds.MS.gene07386.t1;Parent=MS.gene07386.t1
chr1.2 exon 64471560 64471837 64471560 ID=MS.gene07386.t1.exon4;Parent=MS.gene07386.t1
chr1.2 CDS 64471560 64471837 64471560 ID=cds.MS.gene07386.t1;Parent=MS.gene07386.t1
chr1.2 exon 64471309 64471444 64471309 ID=MS.gene07386.t1.exon5;Parent=MS.gene07386.t1
chr1.2 CDS 64471309 64471444 64471309 ID=cds.MS.gene07386.t1;Parent=MS.gene07386.t1
chr1.2 exon 64471102 64471213 64471102 ID=MS.gene07386.t1.exon6;Parent=MS.gene07386.t1
chr1.2 CDS 64471102 64471213 64471102 ID=cds.MS.gene07386.t1;Parent=MS.gene07386.t1
chr1.2 exon 64470827 64470921 64470827 ID=MS.gene07386.t1.exon7;Parent=MS.gene07386.t1
chr1.2 CDS 64470827 64470921 64470827 ID=cds.MS.gene07386.t1;Parent=MS.gene07386.t1
Gene Sequence

>MS.gene07386

ATGGCTAAAGACCCTGTTCGTGTTCTTGTCACTGGTGCTGCAGGTAACCATAATAAATTCTGGGTTTCTCTCAAAATTATGTCTTTTGTTTTGTTTAAGGATATTTTATTATCATATTTTTTTTTATCAGATCTGATCTGATCCTTGTATCATGTTTCTTGATTGGTTTTATTTAGAAATAGAACTATGTTTGGTTTATAATTGCATTAGCTCTTGATTTCTCATGATATGTGCTTACTCTATGATCAATTAAGAAAATAAAAAATCCTAGTAGTTGCAAGCAGTTAATATATTTTTTAAAAAATTTATATGCATTTACTTTCATTTGTTAAAAATAGATCCCTTATAATTATGCAATTTTCTGATAGTATACCTCAATTGTAGTATTTTCCCTATTTGAGAGTTTATAGAAATAACATATGACATGTTTATAAGTTGTTTTCAGTTTATTTCCATAATCTCTCTAGGATAACTTGTGAAAAAAAGCTTGTAGTATATATGAAAACAATTTGTCTTTATTTTAACTTTTGATATAGAAATAGCTTATGCATAATCATTTATACATTTTAAGCATTTATGCTGTAAGTGTTTAGTGAAGTTGTTTATCCAAATATGGTTAAAATGTTTAAAAAAGCAATTTTTGATTTCAACATAAAATCTTTGAGGCATATTTTTTTTTGTACTAAAAAATGTTTTGCCAATTTTAGGACAAATTGGATATGCTCTTGTCCCTATGATTGCCAGGGGAGTGATGCTCGGCCCTGACCAGCCCGTGATCCTCCACATGCTTGACATCCCACCAGCAGCCGAATCACTGAACGGTGTTAAAATGGAGTTGGTGGATGCTGCATTCCCTCTTCTTAAAGGTTTGAGTTTTATTCTTTGCTAAATGTTACTTGAACTTCTTGGACAGCCATATTTCTGGTTTGAAGTTGGATTGTGTTGGTTGATTAAATGCTTTGCAAGATTAAAAGGTATTGTGAAAATTATTTGTTTCAGGTGTTGTTGCTACAACTGATGTGGTTGAGGGATGCACTGGAGTCAATATTGCTGTTATGGTTGGTGGATTCCCTAGAAAAGAAGGTATGGAGAGGAAAGATGTGATGACAAAAAATGTCTCTATTTACAAGTCCCAAGCTTCTGCTCTTGAGAAACATGCTGCTGCAAACTGCAAGGTGATCCTTTAATTCTGAATGCTCAAGCTTTTGTCTTAATTAATTTCAATTTCTAGTTCTGCCGCATTTTATGTCAAAAATATGTTCCATTCTATGGTTCCTCCTCCCCCTTTTTCATGTATCTCTTTCAGACCTGTTTGGATTGACTTATTTGAACTTATATACTACCATAAGCAAGCACTTGTGAGACTGCTTGGGAGAGCTCATGGAAACAGCTCATGACATGTTCGTGAGATGTTTTCAGCTTATTTCCATAAGCTCTACAGGATAGCTTATGAAAACAGTTCATAGTTTATATGAAAACAATTTGACTTTATTTTATTGTTTTTTATAGAAATAGCTTACACATAAGCACTTATGCCATACGAATTTAATTAAGTTGTTGGCTGATCGATTGATTGAACGCTATTATTGTCATAGTTTTTGTGTGATAAGTGTAAAGCCAATACATTACCTGCACTGAATTTATTTTAGTTTAGTGCTGAAAAAGTGGTTCCATCTGGTTGGTTCATGTGTATTTTGGTCATATATGGCAAAGTCCTTGTGATTCCATTCTGGTGAAATCTGTTTCTTTTCTTCACAGAAAATGAGTAGTCCTTTTTACATATTTTTGGAAACATCAATTACATTTTATGTATTTCCTTACATTTCTTTAAACTCTGTATCACCATGCAGGTTCTCGTTGTTGCCAACCCGGCAAACACCAATGCCTTGATCTTGAAGGAATTCGCTCCATCCATTCCTGAGAAAAACATTTCTTGTTTGACAAGATTGGACCATAACAGGGCTCTGGGTCAAATTTCTGAAAGACTGAACGTTGAAGTTTCTAATGTGAAAAATGTTATAATTTGGGGCAACCATTCATCAAGTCAGTACCCTGATGTCAACCACGCAACTGTTAAAATCGCTTCTGCAGAAAAGCCTGTCCGTGAACTCGTGGCTGATGATGCCTGGTATGTTGATGGACAAATACAATGACTTGCAATTTAAACTTTTTTTTACGTATTTATTACATTTTTTTTTCCATTTTGCATTTACTGAAGTATTGATGTAATTTTCATCGTCAAGGTTAAATGGAGAATTCATAGCTACCGTCCAACAACGGGGTGCTGCAATCATTAAAGCTAGAAAGCTTTCAAGTGCACTATCTGCCGCTAGCGCTGCTTGTGACCACATTAGAGATTGGGTTCTAGGAACTCCTGAGGTTGGTTATGTCTTGCTCAATTTTTAATTCAAATTCTTTATATGGAATGACTTCAACTAAGGTTTATGTTTATCTTTTCATTGCAATATCATCAGGGAACCTGGGTATCGATGGGAGTTTATTCTGATGGATCTTACAATGTACCTGCTGGACTAATCTATTCATTCCCTGTCACAACCCGCAATGGCGAATGGCAAATAGTTCAAGGTAAGATTTTGATAAGTTATTACTGGCTCTTGAATTCTTATTGTTTAAAGTGTTAATACTTCATACTTAACGTTTGTCCGTCTTTTATTTGTATTAAGGCATAGTTGTTTTATATAGCAAATAAAGGTTTGTTTTGTGTATGTATCTGATGTAAGTTAATTTGTCTAATTTTTTAACCAGGACTTTCAATTGATGAGTTTTCAAGGAAAAAATTGGACTTGACAGCAGAAGAGCTTTCTGAGGAGAAGGCTTTGGCTTACTCATGCCTCTCTTAG
Protein sequence

>MS.gene07386.t1

MAKDPVRVLVTGAAGQIGYALVPMIARGVMLGPDQPVILHMLDIPPAAESLNGVKMELVDAAFPLLKGVVATTDVVEGCTGVNIAVMVGGFPRKEGMERKDVMTKNVSIYKSQASALEKHAAANCKVLVVANPANTNALILKEFAPSIPEKNISCLTRLDHNRALGQISERLNVEVSNVKNVIIWGNHSSSQYPDVNHATVKIASAEKPVRELVADDAWLNGEFIATVQQRGAAIIKARKLSSALSAASAACDHIRDWVLGTPEGTWVSMGVYSDGSYNVPAGLIYSFPVTTRNGEWQIVQGLSIDEFSRKKLDLTAEELSEEKALAYSCLS