Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene073887.t1 | XP_024630846.1 | 99.2 | 479 | 4 | 0 | 1 | 479 | 6 | 484 | 1.20E-273 | 951.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene073887.t1 | Q9AV81 | 74.2 | 481 | 119 | 3 | 1 | 479 | 6 | 483 | 5.7e-208 | 724.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene073887.t1 | G7I632 | 99.2 | 479 | 4 | 0 | 1 | 479 | 4 | 482 | 8.6e-274 | 951.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049180 | MS.gene073887 | 0.863038 | 3.31E-64 | -1.69E-46 |
| MS.gene050741 | MS.gene073887 | 0.813026 | 3.15E-51 | -1.69E-46 |
| MS.gene053443 | MS.gene073887 | 0.81343 | 2.57E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 99 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAATCAATCGAATAGGATTT+TGG | 0.110325 | 1.1:-27776820 | MS.gene073887:intron |
| GACTGCAATGCTGCTCTTTC+CGG | 0.154596 | 1.1:-27773593 | MS.gene073887:CDS |
| GCATTAGCTGTCACAGAATT+TGG | 0.171506 | 1.1:+27773896 | None:intergenic |
| ACAGAATTTGGAGTAGAAAT+AGG | 0.203246 | 1.1:+27773908 | None:intergenic |
| CATCCCGATGGTCTTATTCT+TGG | 0.266936 | 1.1:-27770344 | MS.gene073887:CDS |
| AAAACTCTTCCTGATTTATC+TGG | 0.271266 | 1.1:-27769192 | MS.gene073887:CDS |
| GTGCAGGCAGCAAGCATTCC+TGG | 0.291861 | 1.1:-27776569 | MS.gene073887:CDS |
| TCTTGATATCCTTCATTCTA+AGG | 0.302903 | 1.1:-27773288 | MS.gene073887:intron |
| TTTGGGAAGTGTCCAGTTAC+AGG | 0.305772 | 1.1:-27776802 | MS.gene073887:CDS |
| CGTGATTGCAAGGCTTAAAA+AGG | 0.320139 | 1.1:-27773978 | MS.gene073887:CDS |
| TTTGACACCACAGGCTCATC+TGG | 0.321677 | 1.1:+27779202 | None:intergenic |
| GGATCCTACCTTGCAGTTGC+AGG | 0.322521 | 1.1:-27769775 | MS.gene073887:CDS |
| ATTTCAGATTCCTGCGACTT+TGG | 0.324712 | 1.1:-27773390 | MS.gene073887:intron |
| CAGATTCTCTTGTTAAGATC+TGG | 0.328133 | 1.1:-27770310 | MS.gene073887:CDS |
| GTTGGAGTTGCTGAGTCATA+TGG | 0.342835 | 1.1:+27769921 | None:intergenic |
| CAGTTGCAGGCTCTGATGTT+AGG | 0.348094 | 1.1:-27769762 | MS.gene073887:intron |
| GCAAATGTTGCTAAGTTTGA+TGG | 0.357923 | 1.1:-27770178 | MS.gene073887:CDS |
| GGAATGCTTGCTGCCTGCAC+TGG | 0.360377 | 1.1:+27776572 | None:intergenic |
| CTCATGATGGTGTAAAGCTT+TGG | 0.362749 | 1.1:-27769980 | MS.gene073887:CDS |
| TTTGTGACTGCATCACTTGA+TGG | 0.370664 | 1.1:-27770686 | MS.gene073887:CDS |
| TGCAGTCACAAAGTAATTAT+TGG | 0.371362 | 1.1:+27770697 | None:intergenic |
| CTTGCTGCCTGCACTGGTCT+AGG | 0.374275 | 1.1:+27776578 | None:intergenic |
| ACTGCAATGCTGCTCTTTCC+GGG | 0.374696 | 1.1:-27773592 | MS.gene073887:CDS |
| GGATGTAATTTCTTCGCATT+AGG | 0.388848 | 1.1:+27773650 | None:intergenic |
| ACTCAGCGGTCACTCTAAAA+AGG | 0.394859 | 1.1:-27773005 | MS.gene073887:intron |
| AAGCGTGAAGTTTGTAGGTC+AGG | 0.396085 | 1.1:-27772899 | MS.gene073887:CDS |
| CTTAAACAAAACTAGCAAAC+AGG | 0.402528 | 1.1:-27773321 | MS.gene073887:CDS |
| CTTCCTGATTTATCTGGAAC+AGG | 0.403899 | 1.1:-27769186 | None:intergenic |
| CAGGGTGAATCTATCATAAC+TGG | 0.405085 | 1.1:-27772880 | MS.gene073887:CDS |
| GATATTGTGCCGATCAAAAC+AGG | 0.410359 | 1.1:-27776760 | MS.gene073887:CDS |
| GTAAGCGGCTCTCCTGTAAC+TGG | 0.411774 | 1.1:+27776790 | None:intergenic |
| TCATGATGGTGTAAAGCTTT+GGG | 0.421276 | 1.1:-27769979 | MS.gene073887:CDS |
| CTGCTTGCCGCGTGATTGCA+AGG | 0.421314 | 1.1:-27773988 | MS.gene073887:CDS |
| TATTGCAGACAGTTCGTTTG+TGG | 0.428520 | 1.1:-27772433 | MS.gene073887:intron |
| ATTAATCGAGAGGCATATAA+TGG | 0.430404 | 1.1:-27779152 | MS.gene073887:intron |
| CATAACTGGTTCAGCAGATA+AGG | 0.431703 | 1.1:-27772866 | MS.gene073887:intron |
| TGTTAGCTCAGAAATTATAC+TGG | 0.433157 | 1.1:+27773616 | None:intergenic |
| TTACAGGACATAATCGCAAC+TGG | 0.446375 | 1.1:-27773085 | MS.gene073887:intron |
| ATTATACTGGAAGATATTCC+AGG | 0.453120 | 1.1:+27773629 | None:intergenic |
| CAGAATTTGGAGTAGAAATA+GGG | 0.462176 | 1.1:+27773909 | None:intergenic |
| CTGCATCACTTGATGGCAGC+TGG | 0.462700 | 1.1:-27770679 | MS.gene073887:CDS |
| TACAGGAGAGCCGCTTACTC+TGG | 0.463440 | 1.1:-27776785 | MS.gene073887:CDS |
| AGATTCTCTTGTTAAGATCT+GGG | 0.470622 | 1.1:-27770309 | MS.gene073887:CDS |
| GAGTCAATACCAGAGTTTGT+TGG | 0.471796 | 1.1:+27769903 | None:intergenic |
| GCAAGCATTCCTGGCATGCT+TGG | 0.474027 | 1.1:-27776560 | MS.gene073887:CDS |
| GTAACAAGCGTGAAGTTTGT+AGG | 0.474487 | 1.1:-27772904 | MS.gene073887:CDS |
| GTAGTATCCATTCTCAGAAA+AGG | 0.476806 | 1.1:+27770129 | None:intergenic |
| ATGCTCCTGTTCATAGCAAT+GGG | 0.482893 | 1.1:-27773877 | MS.gene073887:CDS |
| AGACAGTTCGTTTGTGGCAA+GGG | 0.486431 | 1.1:-27772427 | MS.gene073887:CDS |
| AGCTGCAGATGTATAGCCTT+CGG | 0.487652 | 1.1:+27770370 | None:intergenic |
| ATGCTGCTCTTTCCGGGAAG+AGG | 0.487878 | 1.1:-27773586 | MS.gene073887:CDS |
| CTCATTTATAGGAATGGGAT+GGG | 0.490049 | 1.1:-27776315 | MS.gene073887:intron |
| GATGGTCTTATTCTTGGAAC+TGG | 0.491366 | 1.1:-27770338 | MS.gene073887:CDS |
| CAGACAGTTCGTTTGTGGCA+AGG | 0.499376 | 1.1:-27772428 | MS.gene073887:CDS |
| AAAGTAATACCTTAGAATGA+AGG | 0.500411 | 1.1:+27773279 | None:intergenic |
| AATGCTCCTGTTCATAGCAA+TGG | 0.505400 | 1.1:-27773878 | MS.gene073887:CDS |
| TGGAACATTCCAAGCATGCC+AGG | 0.505625 | 1.1:+27776551 | None:intergenic |
| AGCTGCTGATGACGAGGAGT+TGG | 0.507322 | 1.1:-27773675 | MS.gene073887:CDS |
| CCTCATTTATAGGAATGGGA+TGG | 0.507870 | 1.1:-27776316 | MS.gene073887:intron |
| TTGGAGTTGCTGAGTCATAT+GGG | 0.509156 | 1.1:+27769922 | None:intergenic |
| GTACCTGTTCCAGATAAATC+AGG | 0.512887 | 1.1:+27769183 | None:intergenic |
| CAAAGTAATTATTGGTGGCA+TGG | 0.525985 | 1.1:+27770705 | None:intergenic |
| CTAAGTTTGATGGACATGTC+GGG | 0.530267 | 1.1:-27770168 | MS.gene073887:CDS |
| AGTGAAGCCTAGACCAGTGC+AGG | 0.532479 | 1.1:-27776585 | MS.gene073887:CDS |
| AGCGTGAAGTTTGTAGGTCA+GGG | 0.533196 | 1.1:-27772898 | MS.gene073887:CDS |
| AAAAGGAACGAGACGAAGCT+AGG | 0.533326 | 1.1:-27773961 | MS.gene073887:CDS |
| GTTCCAAGAATAAGACCATC+GGG | 0.534047 | 1.1:+27770341 | None:intergenic |
| TAACTGCAAACAGATCTTGA+AGG | 0.534904 | 1.1:-27772387 | MS.gene073887:CDS |
| GTTCATAGCAATGGGAAAAG+AGG | 0.541436 | 1.1:-27773869 | MS.gene073887:intron |
| TTGCAAATGTGAAATCTGAA+TGG | 0.541920 | 1.1:-27769224 | MS.gene073887:CDS |
| GTAGCTAATACTTGTCCTGA+TGG | 0.546408 | 1.1:+27773028 | None:intergenic |
| TTGTGGCAAGGGTCAGATGA+TGG | 0.550255 | 1.1:-27772416 | MS.gene073887:CDS |
| TCAACAGAAGCCAAAGTCGC+AGG | 0.550956 | 1.1:+27773380 | None:intergenic |
| TTCAGGAACTTGTCTGACTC+AGG | 0.555913 | 1.1:-27770639 | MS.gene073887:intron |
| AGCTAGGTCTATACTTGCAC+AGG | 0.561984 | 1.1:-27773945 | MS.gene073887:CDS |
| TAGGTTTCTGACTCTTCCGA+AGG | 0.569370 | 1.1:-27770386 | MS.gene073887:CDS |
| CTATACTTGCACAGGCAGAA+AGG | 0.575588 | 1.1:-27773937 | MS.gene073887:CDS |
| TCAGCAACTCCAACAAACTC+TGG | 0.577891 | 1.1:-27769912 | MS.gene073887:intron |
| AGGCTCATCTGGAACCTCAC+CGG | 0.580055 | 1.1:+27779213 | None:intergenic |
| AGAGCCTGCAACTGCAAGGT+AGG | 0.584256 | 1.1:+27769771 | None:intergenic |
| GTTAAGTCATGCTCTATACC+AGG | 0.586754 | 1.1:-27776239 | MS.gene073887:intron |
| AATGCGAAGAAATTACATCC+TGG | 0.591581 | 1.1:-27773647 | MS.gene073887:CDS |
| AGTCACAAAGTAATTATTGG+TGG | 0.592605 | 1.1:+27770700 | None:intergenic |
| GCTAAGTTTGATGGACATGT+CGG | 0.592762 | 1.1:-27770169 | MS.gene073887:CDS |
| AGTTCCAAGAATAAGACCAT+CGG | 0.599921 | 1.1:+27770340 | None:intergenic |
| CTAATACTTGTCCTGATGGA+CGG | 0.602167 | 1.1:+27773032 | None:intergenic |
| CATCAGAGCCTGCAACTGCA+AGG | 0.620846 | 1.1:+27769767 | None:intergenic |
| TCGAGAAGAGATTAATCGAG+AGG | 0.622459 | 1.1:-27779162 | MS.gene073887:CDS |
| ACAATATCATCCAGAGTAAG+CGG | 0.631161 | 1.1:+27776775 | None:intergenic |
| AGCAGCAGTTGCATACAGCA+AGG | 0.634251 | 1.1:-27776267 | MS.gene073887:CDS |
| TGATAGACCGCAGCTCATGA+TGG | 0.652388 | 1.1:-27769993 | MS.gene073887:intron |
| TGTGCCGATCAAAACAGGCA+AGG | 0.675176 | 1.1:-27776755 | MS.gene073887:intron |
| TTAAACAAAACTAGCAAACA+GGG | 0.680512 | 1.1:-27773320 | MS.gene073887:CDS |
| CTTTACACCATCATGAGCTG+CGG | 0.684333 | 1.1:+27769986 | None:intergenic |
| GCAGTGGAGTTTGACCACAG+TGG | 0.700459 | 1.1:-27769796 | MS.gene073887:intron |
| TGAGGTTCCAGATGAGCCTG+TGG | 0.703744 | 1.1:-27779209 | MS.gene073887:CDS |
| TGAGAATGGATACTACCTTG+CGG | 0.717359 | 1.1:-27770122 | MS.gene073887:intron |
| CAAGTATTAGCTACACTCAG+CGG | 0.734442 | 1.1:-27773019 | MS.gene073887:CDS |
| CTGCAAGGTAGGATCCACTG+TGG | 0.800585 | 1.1:+27769782 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.1 | gene | 27769187 | 27779238 | 27769187 | ID=MS.gene073887 |
| chr1.1 | mRNA | 27769187 | 27779238 | 27769187 | ID=MS.gene073887.t1;Parent=MS.gene073887 |
| chr1.1 | exon | 27779153 | 27779238 | 27779153 | ID=MS.gene073887.t1.exon1;Parent=MS.gene073887.t1 |
| chr1.1 | CDS | 27779153 | 27779238 | 27779153 | ID=cds.MS.gene073887.t1;Parent=MS.gene073887.t1 |
| chr1.1 | exon | 27776756 | 27776827 | 27776756 | ID=MS.gene073887.t1.exon2;Parent=MS.gene073887.t1 |
| chr1.1 | CDS | 27776756 | 27776827 | 27776756 | ID=cds.MS.gene073887.t1;Parent=MS.gene073887.t1 |
| chr1.1 | exon | 27776547 | 27776609 | 27776547 | ID=MS.gene073887.t1.exon3;Parent=MS.gene073887.t1 |
| chr1.1 | CDS | 27776547 | 27776609 | 27776547 | ID=cds.MS.gene073887.t1;Parent=MS.gene073887.t1 |
| chr1.1 | exon | 27776240 | 27776326 | 27776240 | ID=MS.gene073887.t1.exon4;Parent=MS.gene073887.t1 |
| chr1.1 | CDS | 27776240 | 27776326 | 27776240 | ID=cds.MS.gene073887.t1;Parent=MS.gene073887.t1 |
| chr1.1 | exon | 27773870 | 27774017 | 27773870 | ID=MS.gene073887.t1.exon5;Parent=MS.gene073887.t1 |
| chr1.1 | CDS | 27773870 | 27774017 | 27773870 | ID=cds.MS.gene073887.t1;Parent=MS.gene073887.t1 |
| chr1.1 | exon | 27773574 | 27773695 | 27773574 | ID=MS.gene073887.t1.exon6;Parent=MS.gene073887.t1 |
| chr1.1 | CDS | 27773574 | 27773695 | 27773574 | ID=cds.MS.gene073887.t1;Parent=MS.gene073887.t1 |
| chr1.1 | exon | 27773289 | 27773405 | 27773289 | ID=MS.gene073887.t1.exon7;Parent=MS.gene073887.t1 |
| chr1.1 | CDS | 27773289 | 27773405 | 27773289 | ID=cds.MS.gene073887.t1;Parent=MS.gene073887.t1 |
| chr1.1 | exon | 27773006 | 27773101 | 27773006 | ID=MS.gene073887.t1.exon8;Parent=MS.gene073887.t1 |
| chr1.1 | CDS | 27773006 | 27773101 | 27773006 | ID=cds.MS.gene073887.t1;Parent=MS.gene073887.t1 |
| chr1.1 | exon | 27772867 | 27772926 | 27772867 | ID=MS.gene073887.t1.exon9;Parent=MS.gene073887.t1 |
| chr1.1 | CDS | 27772867 | 27772926 | 27772867 | ID=cds.MS.gene073887.t1;Parent=MS.gene073887.t1 |
| chr1.1 | exon | 27772373 | 27772447 | 27772373 | ID=MS.gene073887.t1.exon10;Parent=MS.gene073887.t1 |
| chr1.1 | CDS | 27772373 | 27772447 | 27772373 | ID=cds.MS.gene073887.t1;Parent=MS.gene073887.t1 |
| chr1.1 | exon | 27770640 | 27770744 | 27770640 | ID=MS.gene073887.t1.exon11;Parent=MS.gene073887.t1 |
| chr1.1 | CDS | 27770640 | 27770744 | 27770640 | ID=cds.MS.gene073887.t1;Parent=MS.gene073887.t1 |
| chr1.1 | exon | 27770295 | 27770405 | 27770295 | ID=MS.gene073887.t1.exon12;Parent=MS.gene073887.t1 |
| chr1.1 | CDS | 27770295 | 27770405 | 27770295 | ID=cds.MS.gene073887.t1;Parent=MS.gene073887.t1 |
| chr1.1 | exon | 27770123 | 27770200 | 27770123 | ID=MS.gene073887.t1.exon13;Parent=MS.gene073887.t1 |
| chr1.1 | CDS | 27770123 | 27770200 | 27770123 | ID=cds.MS.gene073887.t1;Parent=MS.gene073887.t1 |
| chr1.1 | exon | 27769913 | 27770009 | 27769913 | ID=MS.gene073887.t1.exon14;Parent=MS.gene073887.t1 |
| chr1.1 | CDS | 27769913 | 27770009 | 27769913 | ID=cds.MS.gene073887.t1;Parent=MS.gene073887.t1 |
| chr1.1 | exon | 27769763 | 27769814 | 27769763 | ID=MS.gene073887.t1.exon15;Parent=MS.gene073887.t1 |
| chr1.1 | CDS | 27769763 | 27769814 | 27769763 | ID=cds.MS.gene073887.t1;Parent=MS.gene073887.t1 |
| chr1.1 | exon | 27769187 | 27769257 | 27769187 | ID=MS.gene073887.t1.exon16;Parent=MS.gene073887.t1 |
| chr1.1 | CDS | 27769187 | 27769257 | 27769187 | ID=cds.MS.gene073887.t1;Parent=MS.gene073887.t1 |
| Gene Sequence |
| Protein sequence |
