Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene074424.t1 | XP_013444456.1 | 100 | 62 | 0 | 0 | 21 | 82 | 68 | 129 | 2.90E-26 | 127.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene074424.t1 | A0A072TMQ7 | 100.0 | 62 | 0 | 0 | 21 | 82 | 68 | 129 | 2.1e-26 | 127.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050800 | MS.gene074424 | 0.83297 | 6.98E-56 | -1.69E-46 |
| MS.gene051113 | MS.gene074424 | 0.93625 | 2.34E-97 | -1.69E-46 |
| MS.gene051387 | MS.gene074424 | -0.818099 | 2.34E-52 | -1.69E-46 |
| MS.gene056448 | MS.gene074424 | -0.803993 | 2.67E-49 | -1.69E-46 |
| MS.gene056942 | MS.gene074424 | -0.80827 | 3.36E-50 | -1.69E-46 |
| MS.gene060804 | MS.gene074424 | -0.805907 | 1.06E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene074424.t1 | MTR_8g021025 | 100.000 | 62 | 0 | 0 | 21 | 82 | 68 | 129 | 2.89e-41 | 131 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 25 sgRNAs with CRISPR-Local
Find 31 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACCTTCCGCCGTCTGAATA+TGG | 0.240508 | 8.2:-73971555 | None:intergenic |
| CAAAAGTGCTTTCCATAAAA+AGG | 0.284199 | 8.2:-73971772 | None:intergenic |
| TCTACTTATCCTTCTCGGTT+TGG | 0.359316 | 8.2:+73971818 | MS.gene074424:CDS |
| ACTTATCTACTTATCCTTCT+CGG | 0.384698 | 8.2:+73971813 | MS.gene074424:CDS |
| TAGGTTATTGAAACGGCTTA+AGG | 0.428816 | 8.2:+73971682 | MS.gene074424:intron |
| GAATATGGAGACCTTGCGGC+GGG | 0.492635 | 8.2:-73971540 | None:intergenic |
| AATATCTTAGGTTATTGAAA+CGG | 0.511068 | 8.2:+73971675 | MS.gene074424:intron |
| CTTGATAATTTACATGAAGA+GGG | 0.523231 | 8.2:-73971858 | None:intergenic |
| ACTTGATAATTTACATGAAG+AGG | 0.529374 | 8.2:-73971859 | None:intergenic |
| GTGAAAGATCTTATTGAAGC+TGG | 0.539200 | 8.2:+73971722 | MS.gene074424:CDS |
| ATTTACATGAAGAGGGTTCG+AGG | 0.539387 | 8.2:-73971851 | None:intergenic |
| AACATGGCAGCCCCGCCGCA+AGG | 0.546242 | 8.2:+73971529 | None:intergenic |
| GCGTTGTTGAAAAGTTTCAA+TGG | 0.549801 | 8.2:-73971750 | None:intergenic |
| CTCCATATTCAGACGGCGGA+AGG | 0.556606 | 8.2:+73971553 | MS.gene074424:CDS |
| AAGGTTCAAACGGTGAGGCT+AGG | 0.576644 | 8.2:+73971572 | MS.gene074424:CDS |
| CAGACGGCGGAAGGTTCAAA+CGG | 0.612212 | 8.2:+73971562 | MS.gene074424:CDS |
| TAATCTGAACCAAACCGAGA+AGG | 0.614730 | 8.2:-73971827 | None:intergenic |
| TGAATATGGAGACCTTGCGG+CGG | 0.640070 | 8.2:-73971541 | None:intergenic |
| TAGATAAGTTAACACCAAGA+GGG | 0.640424 | 8.2:-73971799 | None:intergenic |
| GTAGATAAGTTAACACCAAG+AGG | 0.647293 | 8.2:-73971800 | None:intergenic |
| GCAAGGTCTCCATATTCAGA+CGG | 0.653088 | 8.2:+73971546 | MS.gene074424:CDS |
| GTCTGAATATGGAGACCTTG+CGG | 0.668375 | 8.2:-73971544 | None:intergenic |
| GGCGGAAGGTTCAAACGGTG+AGG | 0.671562 | 8.2:+73971567 | MS.gene074424:CDS |
| AATATGGAGACCTTGCGGCG+GGG | 0.701594 | 8.2:-73971539 | None:intergenic |
| AGGTCTCCATATTCAGACGG+CGG | 0.717616 | 8.2:+73971549 | MS.gene074424:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATATCTTAGGTTATTGAAA+CGG | + | chr8.2:73971675-73971694 | MS.gene074424:intron | 20.0% |
| ! | GCATAGTTAGAATGTTTAAA+AGG | + | chr8.2:73971627-73971646 | MS.gene074424:intron | 25.0% |
| ACTTATCTACTTATCCTTCT+CGG | + | chr8.2:73971813-73971832 | MS.gene074424:CDS | 30.0% | |
| GGCTTAAGTTACAATATCTT+AGG | + | chr8.2:73971663-73971682 | MS.gene074424:intron | 30.0% | |
| TAGATAAGTTAACACCAAGA+GGG | - | chr8.2:73971802-73971821 | None:intergenic | 30.0% | |
| ! | TTTTCAACAACGCCTTTTTA+TGG | + | chr8.2:73971760-73971779 | MS.gene074424:CDS | 30.0% |
| !! | CAAAAGTGCTTTCCATAAAA+AGG | - | chr8.2:73971775-73971794 | None:intergenic | 30.0% |
| GCGTTGTTGAAAAGTTTCAA+TGG | - | chr8.2:73971753-73971772 | None:intergenic | 35.0% | |
| GTAGATAAGTTAACACCAAG+AGG | - | chr8.2:73971803-73971822 | None:intergenic | 35.0% | |
| GTGAAAGATCTTATTGAAGC+TGG | + | chr8.2:73971722-73971741 | MS.gene074424:CDS | 35.0% | |
| TAGGTTATTGAAACGGCTTA+AGG | + | chr8.2:73971682-73971701 | MS.gene074424:intron | 35.0% | |
| TTAAAAGGATGCGTGTAAAG+TGG | + | chr8.2:73971642-73971661 | MS.gene074424:intron | 35.0% | |
| TAATCTGAACCAAACCGAGA+AGG | - | chr8.2:73971830-73971849 | None:intergenic | 40.0% | |
| TCTACTTATCCTTCTCGGTT+TGG | + | chr8.2:73971818-73971837 | MS.gene074424:CDS | 40.0% | |
| AAACCACGGCGGAGATTAAT+TGG | + | chr8.2:73971602-73971621 | MS.gene074424:intron | 45.0% | |
| GCAAGGTCTCCATATTCAGA+CGG | + | chr8.2:73971546-73971565 | MS.gene074424:CDS | 45.0% | |
| GTCTGAATATGGAGACCTTG+CGG | - | chr8.2:73971547-73971566 | None:intergenic | 45.0% | |
| AACCTTCCGCCGTCTGAATA+TGG | - | chr8.2:73971558-73971577 | None:intergenic | 50.0% | |
| AAGGTTCAAACGGTGAGGCT+AGG | + | chr8.2:73971572-73971591 | MS.gene074424:CDS | 50.0% | |
| AGGTCTCCATATTCAGACGG+CGG | + | chr8.2:73971549-73971568 | MS.gene074424:CDS | 50.0% | |
| GGCTAGGTAGCAAGAAACCA+CGG | + | chr8.2:73971588-73971607 | MS.gene074424:intron | 50.0% | |
| TAGGTAGCAAGAAACCACGG+CGG | + | chr8.2:73971591-73971610 | MS.gene074424:intron | 50.0% | |
| TGAATATGGAGACCTTGCGG+CGG | - | chr8.2:73971544-73971563 | None:intergenic | 50.0% | |
| !! | AGCACTTTTGCTGTCCCTCT+TGG | + | chr8.2:73971785-73971804 | MS.gene074424:CDS | 50.0% |
| AATATGGAGACCTTGCGGCG+GGG | - | chr8.2:73971542-73971561 | None:intergenic | 55.0% | |
| CAGACGGCGGAAGGTTCAAA+CGG | + | chr8.2:73971562-73971581 | MS.gene074424:CDS | 55.0% | |
| CCACCAATTAATCTCCGCCG+TGG | - | chr8.2:73971608-73971627 | None:intergenic | 55.0% | |
| CCACGGCGGAGATTAATTGG+TGG | + | chr8.2:73971605-73971624 | MS.gene074424:intron | 55.0% | |
| CTCCATATTCAGACGGCGGA+AGG | + | chr8.2:73971553-73971572 | MS.gene074424:CDS | 55.0% | |
| GAATATGGAGACCTTGCGGC+GGG | - | chr8.2:73971543-73971562 | None:intergenic | 55.0% | |
| GGCGGAAGGTTCAAACGGTG+AGG | + | chr8.2:73971567-73971586 | MS.gene074424:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.2 | gene | 73971532 | 73971871 | 73971532 | ID=MS.gene074424 |
| chr8.2 | mRNA | 73971532 | 73971871 | 73971532 | ID=MS.gene074424.t1;Parent=MS.gene074424 |
| chr8.2 | exon | 73971532 | 73971593 | 73971532 | ID=MS.gene074424.t1.exon1;Parent=MS.gene074424.t1 |
| chr8.2 | CDS | 73971532 | 73971593 | 73971532 | ID=cds.MS.gene074424.t1;Parent=MS.gene074424.t1 |
| chr8.2 | exon | 73971685 | 73971871 | 73971685 | ID=MS.gene074424.t1.exon2;Parent=MS.gene074424.t1 |
| chr8.2 | CDS | 73971685 | 73971871 | 73971685 | ID=cds.MS.gene074424.t1;Parent=MS.gene074424.t1 |
| Gene Sequence |
| Protein sequence |
