AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene051113


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene051113.t1 MTR_8g021025 96.899 129 3 1 1 128 1 129 6.61e-88 251
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene051113.t1 AT3G14060 44.037 109 57 3 17 124 16 121 2.58e-19 77.8
MS.gene051113.t1 AT1G54120 41.071 112 63 3 7 115 3 114 4.27e-18 74.7

Find 41 sgRNAs with CRISPR-Local

Find 39 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACCTTCCGCCGTCTGAATA+TGG 0.240508 8.3:-70202383 None:intergenic
CAAAAGTGCTTTCCATAAAA+AGG 0.284199 8.3:-70202600 None:intergenic
GCATAGTTAGAATGTTTAAA+AGG 0.289065 8.3:+70202455 MS.gene051113:CDS
GGCTTAAGTTACAATATCTT+AGG 0.307785 8.3:+70202491 MS.gene051113:CDS
AAACCACGGCGGAGATTAAT+TGG 0.312099 8.3:+70202430 MS.gene051113:CDS
TGTCATGGTGGTGGTGGTGA+TGG 0.342985 8.3:-70202335 None:intergenic
TCTACTTATCCTTCTCGGTT+TGG 0.359316 8.3:+70202646 MS.gene051113:CDS
ACTTATCTACTTATCCTTCT+CGG 0.384698 8.3:+70202641 MS.gene051113:CDS
GTCATGGTGGTGGTGGTGAT+GGG 0.399546 8.3:-70202334 None:intergenic
TAGGTTATTGAAACGGCTTA+AGG 0.428816 8.3:+70202510 MS.gene051113:CDS
CTGCCATGTTGTCATGGTGG+TGG 0.440074 8.3:-70202344 None:intergenic
CCATGTTGTCATGGTGGTGG+TGG 0.486167 8.3:-70202341 None:intergenic
GAATATGGAGACCTTGCGGC+GGG 0.492635 8.3:-70202368 None:intergenic
GCGGGGCTGCCATGTTGTCA+TGG 0.506143 8.3:-70202350 None:intergenic
AATATCTTAGGTTATTGAAA+CGG 0.511068 8.3:+70202503 MS.gene051113:CDS
CTTGATAATTTACATGAAGA+GGG 0.523231 8.3:-70202686 None:intergenic
ACTTGATAATTTACATGAAG+AGG 0.529374 8.3:-70202687 None:intergenic
GTGAAAGATCTTATTGAAGC+TGG 0.539200 8.3:+70202550 MS.gene051113:CDS
ATTTACATGAAGAGGGTTCG+AGG 0.539387 8.3:-70202679 None:intergenic
AACATGGCAGCCCCGCCGCA+AGG 0.546242 8.3:+70202357 MS.gene051113:CDS
GCGTTGTTGAAAAGTTTCAA+TGG 0.549801 8.3:-70202578 None:intergenic
CTCCATATTCAGACGGCGGA+AGG 0.556606 8.3:+70202381 MS.gene051113:CDS
TTAAAAGGATGCGTGTAAAG+TGG 0.560887 8.3:+70202470 MS.gene051113:CDS
AAGGTTCAAACGGTGAGGCT+AGG 0.576644 8.3:+70202400 MS.gene051113:CDS
CAGACGGCGGAAGGTTCAAA+CGG 0.612212 8.3:+70202390 MS.gene051113:CDS
TAATCTGAACCAAACCGAGA+AGG 0.614730 8.3:-70202655 None:intergenic
CCACCACCACCATGACAACA+TGG 0.623806 8.3:+70202341 MS.gene051113:CDS
CCACGGCGGAGATTAATTGG+TGG 0.631671 8.3:+70202433 MS.gene051113:CDS
GGGCTGCCATGTTGTCATGG+TGG 0.634932 8.3:-70202347 None:intergenic
TGAATATGGAGACCTTGCGG+CGG 0.640070 8.3:-70202369 None:intergenic
TAGATAAGTTAACACCAAGA+GGG 0.640424 8.3:-70202627 None:intergenic
GTAGATAAGTTAACACCAAG+AGG 0.647293 8.3:-70202628 None:intergenic
CCACCAATTAATCTCCGCCG+TGG 0.651481 8.3:-70202433 None:intergenic
GCAAGGTCTCCATATTCAGA+CGG 0.653088 8.3:+70202374 MS.gene051113:CDS
TTGTTTGTGATCTAGAGCCA+TGG 0.655355 8.3:-70202311 None:intergenic
GGCTAGGTAGCAAGAAACCA+CGG 0.666237 8.3:+70202416 MS.gene051113:CDS
GTCTGAATATGGAGACCTTG+CGG 0.668375 8.3:-70202372 None:intergenic
GGCGGAAGGTTCAAACGGTG+AGG 0.671562 8.3:+70202395 MS.gene051113:CDS
AATATGGAGACCTTGCGGCG+GGG 0.701594 8.3:-70202367 None:intergenic
AGGTCTCCATATTCAGACGG+CGG 0.717616 8.3:+70202377 MS.gene051113:CDS
TAGGTAGCAAGAAACCACGG+CGG 0.844201 8.3:+70202419 MS.gene051113:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATATCTTAGGTTATTGAAA+CGG + chr8.3:70202503-70202522 MS.gene051113:CDS 20.0%
! GCATAGTTAGAATGTTTAAA+AGG + chr8.3:70202455-70202474 MS.gene051113:CDS 25.0%
ACTTATCTACTTATCCTTCT+CGG + chr8.3:70202641-70202660 MS.gene051113:CDS 30.0%
GGCTTAAGTTACAATATCTT+AGG + chr8.3:70202491-70202510 MS.gene051113:CDS 30.0%
TAGATAAGTTAACACCAAGA+GGG - chr8.3:70202630-70202649 None:intergenic 30.0%
! TTTTCAACAACGCCTTTTTA+TGG + chr8.3:70202588-70202607 MS.gene051113:CDS 30.0%
!! CAAAAGTGCTTTCCATAAAA+AGG - chr8.3:70202603-70202622 None:intergenic 30.0%
GCGTTGTTGAAAAGTTTCAA+TGG - chr8.3:70202581-70202600 None:intergenic 35.0%
GTAGATAAGTTAACACCAAG+AGG - chr8.3:70202631-70202650 None:intergenic 35.0%
GTGAAAGATCTTATTGAAGC+TGG + chr8.3:70202550-70202569 MS.gene051113:CDS 35.0%
TAGGTTATTGAAACGGCTTA+AGG + chr8.3:70202510-70202529 MS.gene051113:CDS 35.0%
TTAAAAGGATGCGTGTAAAG+TGG + chr8.3:70202470-70202489 MS.gene051113:CDS 35.0%
TAATCTGAACCAAACCGAGA+AGG - chr8.3:70202658-70202677 None:intergenic 40.0%
TCTACTTATCCTTCTCGGTT+TGG + chr8.3:70202646-70202665 MS.gene051113:CDS 40.0%
AAACCACGGCGGAGATTAAT+TGG + chr8.3:70202430-70202449 MS.gene051113:CDS 45.0%
GCAAGGTCTCCATATTCAGA+CGG + chr8.3:70202374-70202393 MS.gene051113:CDS 45.0%
GTCTGAATATGGAGACCTTG+CGG - chr8.3:70202375-70202394 None:intergenic 45.0%
AACCTTCCGCCGTCTGAATA+TGG - chr8.3:70202386-70202405 None:intergenic 50.0%
AAGGTTCAAACGGTGAGGCT+AGG + chr8.3:70202400-70202419 MS.gene051113:CDS 50.0%
AGGTCTCCATATTCAGACGG+CGG + chr8.3:70202377-70202396 MS.gene051113:CDS 50.0%
GGCTAGGTAGCAAGAAACCA+CGG + chr8.3:70202416-70202435 MS.gene051113:CDS 50.0%
TAGGTAGCAAGAAACCACGG+CGG + chr8.3:70202419-70202438 MS.gene051113:CDS 50.0%
TGAATATGGAGACCTTGCGG+CGG - chr8.3:70202372-70202391 None:intergenic 50.0%
!! AGCACTTTTGCTGTCCCTCT+TGG + chr8.3:70202613-70202632 MS.gene051113:CDS 50.0%
AATATGGAGACCTTGCGGCG+GGG - chr8.3:70202370-70202389 None:intergenic 55.0%
CAGACGGCGGAAGGTTCAAA+CGG + chr8.3:70202390-70202409 MS.gene051113:CDS 55.0%
CCACCAATTAATCTCCGCCG+TGG - chr8.3:70202436-70202455 None:intergenic 55.0%
CCACCACCACCATGACAACA+TGG + chr8.3:70202341-70202360 MS.gene051113:CDS 55.0%
CCACGGCGGAGATTAATTGG+TGG + chr8.3:70202433-70202452 MS.gene051113:CDS 55.0%
CTCCATATTCAGACGGCGGA+AGG + chr8.3:70202381-70202400 MS.gene051113:CDS 55.0%
CTGCCATGTTGTCATGGTGG+TGG - chr8.3:70202347-70202366 None:intergenic 55.0%
GAATATGGAGACCTTGCGGC+GGG - chr8.3:70202371-70202390 None:intergenic 55.0%
!! CCATGTTGTCATGGTGGTGG+TGG - chr8.3:70202344-70202363 None:intergenic 55.0%
!! GTCATGGTGGTGGTGGTGAT+GGG - chr8.3:70202337-70202356 None:intergenic 55.0%
!! TGTCATGGTGGTGGTGGTGA+TGG - chr8.3:70202338-70202357 None:intergenic 55.0%
GGCGGAAGGTTCAAACGGTG+AGG + chr8.3:70202395-70202414 MS.gene051113:CDS 60.0%
GGGCTGCCATGTTGTCATGG+TGG - chr8.3:70202350-70202369 None:intergenic 60.0%
GCGGGGCTGCCATGTTGTCA+TGG - chr8.3:70202353-70202372 None:intergenic 65.0%
AACATGGCAGCCCCGCCGCA+AGG + chr8.3:70202357-70202376 MS.gene051113:CDS 70.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 70202313 70202699 70202313 ID=MS.gene051113
chr8.3 mRNA 70202313 70202699 70202313 ID=MS.gene051113.t1;Parent=MS.gene051113
chr8.3 exon 70202313 70202699 70202313 ID=MS.gene051113.t1.exon1;Parent=MS.gene051113.t1
chr8.3 CDS 70202313 70202699 70202313 ID=cds.MS.gene051113.t1;Parent=MS.gene051113.t1
Gene Sequence

>MS.gene051113

ATGGCTCTAGATCACAAACAACCCATCACCACCACCACCATGACAACATGGCAGCCCCGCCGCAAGGTCTCCATATTCAGACGGCGGAAGGTTCAAACGGTGAGGCTAGGTAGCAAGAAACCACGGCGGAGATTAATTGGTGGCATAGTTAGAATGTTTAAAAGGATGCGTGTAAAGTGGCTTAAGTTACAATATCTTAGGTTATTGAAACGGCTTAAGGAACATTATAGAAACGTTGTGAAAGATCTTATTGAAGCTGGTGCTACCATTGAAACTTTTCAACAACGCCTTTTTATGGAAAGCACTTTTGCTGTCCCTCTTGGTGTTAACTTATCTACTTATCCTTCTCGGTTTGGTTCAGATTATCCTCGAACCCTCTTCATGTAA

Protein sequence

>MS.gene051113.t1

MALDHKQPITTTTMTTWQPRRKVSIFRRRKVQTVRLGSKKPRRRLIGGIVRMFKRMRVKWLKLQYLRLLKRLKEHYRNVVKDLIEAGATIETFQQRLFMESTFAVPLGVNLSTYPSRFGSDYPRTLFM