Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050800.t1 | XP_003626135.2 | 100 | 54 | 0 | 0 | 1 | 54 | 1 | 54 | 2.40E-21 | 110.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050800.t1 | Q9ZNS6 | 81.5 | 54 | 10 | 0 | 1 | 54 | 1 | 54 | 7.3e-19 | 93.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050800.t1 | Q2HRR6 | 100.0 | 54 | 0 | 0 | 1 | 54 | 1 | 54 | 1.7e-21 | 110.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050800 | MS.gene051113 | 0.840929 | 6.47E-58 | -1.69E-46 |
| MS.gene050800 | MS.gene051694 | -0.813447 | 2.55E-51 | -1.69E-46 |
| MS.gene050800 | MS.gene053596 | 0.821488 | 3.93E-53 | -1.69E-46 |
| MS.gene050800 | MS.gene056942 | -0.800521 | 1.39E-48 | -1.69E-46 |
| MS.gene050800 | MS.gene058157 | -0.805238 | 1.47E-49 | -1.69E-46 |
| MS.gene050800 | MS.gene063742 | 0.823158 | 1.61E-53 | -1.69E-46 |
| MS.gene050800 | MS.gene063743 | 0.823907 | 1.08E-53 | -1.69E-46 |
| MS.gene050800 | MS.gene063746 | 0.806377 | 8.47E-50 | -1.69E-46 |
| MS.gene050800 | MS.gene063747 | 0.810427 | 1.16E-50 | -1.69E-46 |
| MS.gene050800 | MS.gene063748 | 0.824495 | 7.83E-54 | -1.69E-46 |
| MS.gene050800 | MS.gene064062 | 0.818376 | 2.03E-52 | -1.69E-46 |
| MS.gene050800 | MS.gene068009 | 0.830951 | 2.20E-55 | -1.69E-46 |
| MS.gene050800 | MS.gene072079 | 0.805386 | 1.37E-49 | -1.69E-46 |
| MS.gene050800 | MS.gene072324 | 0.833551 | 5.00E-56 | -1.69E-46 |
| MS.gene050800 | MS.gene072602 | 0.934797 | 2.31E-96 | -1.69E-46 |
| MS.gene050800 | MS.gene074424 | 0.83297 | 6.98E-56 | -1.69E-46 |
| MS.gene050800 | MS.gene07524 | -0.816912 | 4.33E-52 | -1.69E-46 |
| MS.gene050800 | MS.gene20448 | -0.804063 | 2.59E-49 | -1.69E-46 |
| MS.gene050800 | MS.gene25114 | 0.830272 | 3.22E-55 | -1.69E-46 |
| MS.gene050800 | MS.gene26726 | 0.822155 | 2.76E-53 | -1.69E-46 |
| MS.gene050800 | MS.gene30407 | 0.841894 | 3.60E-58 | -1.69E-46 |
| MS.gene050800 | MS.gene30911 | 0.808251 | 3.40E-50 | -1.69E-46 |
| MS.gene050800 | MS.gene33504 | -0.815624 | 8.40E-52 | -1.69E-46 |
| MS.gene050800 | MS.gene34092 | -0.813762 | 2.17E-51 | -1.69E-46 |
| MS.gene050800 | MS.gene37743 | 0.840448 | 8.64E-58 | -1.69E-46 |
| MS.gene050800 | MS.gene40322 | 0.869472 | 3.02E-66 | -1.69E-46 |
| MS.gene050800 | MS.gene43940 | 0.807557 | 4.77E-50 | -1.69E-46 |
| MS.gene050800 | MS.gene45287 | -0.808229 | 3.43E-50 | -1.69E-46 |
| MS.gene050800 | MS.gene46900 | 0.800361 | 1.49E-48 | -1.69E-46 |
| MS.gene050800 | MS.gene47828 | -0.818939 | 1.51E-52 | -1.69E-46 |
| MS.gene050800 | MS.gene57812 | 0.825937 | 3.57E-54 | -1.69E-46 |
| MS.gene050800 | MS.gene59273 | 0.809584 | 1.76E-50 | -1.69E-46 |
| MS.gene050800 | MS.gene66304 | 0.826985 | 2.01E-54 | -1.69E-46 |
| MS.gene050800 | MS.gene68121 | 0.800778 | 1.23E-48 | -1.69E-46 |
| MS.gene050800 | MS.gene70732 | -0.804769 | 1.84E-49 | -1.69E-46 |
| MS.gene050800 | MS.gene74906 | 0.807145 | 5.83E-50 | -1.69E-46 |
| MS.gene050800 | MS.gene75310 | 0.876292 | 1.57E-68 | -1.69E-46 |
| MS.gene050800 | MS.gene83460 | -0.806621 | 7.53E-50 | -1.69E-46 |
| MS.gene050800 | MS.gene85380 | -0.801405 | 9.14E-49 | -1.69E-46 |
| MS.gene050800 | MS.gene86841 | -0.809998 | 1.43E-50 | -1.69E-46 |
| MS.gene050800 | MS.gene88000 | 0.808577 | 2.89E-50 | -1.69E-46 |
| MS.gene050800 | MS.gene88405 | 0.800668 | 1.29E-48 | -1.69E-46 |
| MS.gene050800 | MS.gene89531 | -0.824828 | 6.53E-54 | -1.69E-46 |
| MS.gene050800 | MS.gene90245 | 0.819123 | 1.37E-52 | -1.69E-46 |
| MS.gene050800 | MS.gene97182 | 0.818838 | 1.59E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050800.t1 | MTR_7g111450 | 100.000 | 54 | 0 | 0 | 1 | 54 | 1 | 54 | 8.33e-33 | 106 |
| MS.gene050800.t1 | MTR_7g111380 | 87.037 | 54 | 7 | 0 | 1 | 54 | 1 | 54 | 2.10e-29 | 98.2 |
| MS.gene050800.t1 | MTR_7g111350 | 81.481 | 54 | 10 | 0 | 1 | 54 | 1 | 54 | 2.25e-26 | 90.5 |
| MS.gene050800.t1 | MTR_6g033495 | 61.538 | 52 | 20 | 0 | 1 | 52 | 1 | 52 | 1.67e-19 | 73.2 |
| MS.gene050800.t1 | MTR_4g130675 | 62.500 | 48 | 17 | 1 | 6 | 52 | 8 | 55 | 8.08e-14 | 60.5 |
| MS.gene050800.t1 | MTR_4g130660 | 56.522 | 46 | 19 | 1 | 8 | 52 | 10 | 55 | 7.41e-12 | 54.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050800.t1 | AT3G05890 | 81.481 | 54 | 10 | 0 | 1 | 54 | 1 | 54 | 1.83e-26 | 90.9 |
| MS.gene050800.t1 | AT3G05880 | 75.926 | 54 | 13 | 0 | 1 | 54 | 1 | 54 | 3.56e-25 | 87.4 |
| MS.gene050800.t1 | AT2G38905 | 62.264 | 53 | 20 | 0 | 1 | 53 | 1 | 53 | 2.73e-20 | 75.1 |
| MS.gene050800.t1 | AT1G57550 | 50.980 | 51 | 25 | 0 | 4 | 54 | 2 | 52 | 1.47e-15 | 63.2 |
| MS.gene050800.t1 | AT4G28088 | 57.692 | 52 | 21 | 1 | 2 | 52 | 4 | 55 | 9.79e-15 | 61.6 |
| MS.gene050800.t1 | AT2G24040 | 64.583 | 48 | 16 | 1 | 6 | 52 | 8 | 55 | 1.34e-14 | 61.2 |
| MS.gene050800.t1 | AT4G30650 | 65.217 | 46 | 15 | 1 | 8 | 52 | 10 | 55 | 4.59e-14 | 60.1 |
| MS.gene050800.t1 | AT4G30660 | 67.391 | 46 | 14 | 1 | 8 | 52 | 10 | 55 | 2.93e-13 | 58.2 |
| MS.gene050800.t1 | AT4G30660 | 67.391 | 46 | 14 | 1 | 8 | 52 | 10 | 55 | 2.93e-13 | 58.2 |
Find 0 sgRNAs with CRISPR-Local
Find 21 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAATTAATTAATCAATTGTT+TGG | - | 50603:17418-17437 | MS.gene050800:intron | 10.0% |
| !! | CAAAAAAAAAAGAAGAAGAA+AGG | + | 50603:17380-17399 | MS.gene050800:intergenic | 20.0% |
| !! | TTAATTAATCAATTGTTTGG+TGG | - | 50603:17421-17440 | MS.gene050800:intron | 20.0% |
| !! | TTGGTGGTAATGTATTATAT+AGG | - | 50603:17437-17456 | MS.gene050800:intron | 25.0% |
| ! | GTGGTAATGTATTATATAGG+TGG | - | 50603:17440-17459 | MS.gene050800:intron | 30.0% |
| !!! | GGAAGAGAATTTTAGTTACA+TGG | + | 50603:17359-17378 | MS.gene050800:intergenic | 30.0% |
| AGATAGCATAGAGAATTCCA+GGG | + | 50603:17506-17525 | None:intergenic | 35.0% | |
| GTATTATATAGGTGGAGTTC+TGG | - | 50603:17448-17467 | MS.gene050800:intron | 35.0% | |
| TAGATAGCATAGAGAATTCC+AGG | + | 50603:17507-17526 | MS.gene050800:intergenic | 35.0% | |
| !!! | TGTTTGGTGCTAACACTTTT+TGG | - | 50603:17474-17493 | MS.gene050800:CDS | 35.0% |
| TTACATGGCAGCCAAACTTG+AGG | + | 50603:17344-17363 | MS.gene050800:intergenic | 45.0% | |
| !!! | ACACTTTTTGGCTATCTCCC+TGG | - | 50603:17486-17505 | MS.gene050800:CDS | 45.0% |
| AACTTGAGGAAGACACCCAG+AGG | + | 50603:17330-17349 | None:intergenic | 50.0% | |
| CTGGGTGTCTTCCTCAAGTT+TGG | - | 50603:17330-17349 | MS.gene050800:CDS | 50.0% | |
| TGAGGAAGACACCCAGAGGA+GGG | + | 50603:17326-17345 | MS.gene050800:intergenic | 55.0% | |
| TTGAGGAAGACACCCAGAGG+AGG | + | 50603:17327-17346 | MS.gene050800:intergenic | 55.0% | |
| !! | GGTGGAGTTCTGGCTCTGTT+TGG | - | 50603:17458-17477 | MS.gene050800:intron | 55.0% |
| ACCCAGAGGAGGGAGGATGA+TGG | + | 50603:17316-17335 | None:intergenic | 60.0% | |
| GCCATCATCCTCCCTCCTCT+GGG | - | 50603:17312-17331 | MS.gene050800:CDS | 60.0% | |
| TGCCATCATCCTCCCTCCTC+TGG | - | 50603:17311-17330 | MS.gene050800:CDS | 60.0% | |
| GGAAGACACCCAGAGGAGGG+AGG | + | 50603:17323-17342 | MS.gene050800:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 50603 | gene | 17279 | 17542 | 17279 | ID=MS.gene050800 |
| 50603 | mRNA | 17279 | 17542 | 17279 | ID=MS.gene050800.t1;Parent=MS.gene050800 |
| 50603 | exon | 17462 | 17542 | 17462 | ID=MS.gene050800.t1.exon1;Parent=MS.gene050800.t1 |
| 50603 | CDS | 17462 | 17542 | 17462 | ID=cds.MS.gene050800.t1;Parent=MS.gene050800.t1 |
| 50603 | exon | 17279 | 17362 | 17279 | ID=MS.gene050800.t1.exon2;Parent=MS.gene050800.t1 |
| 50603 | CDS | 17279 | 17362 | 17279 | ID=cds.MS.gene050800.t1;Parent=MS.gene050800.t1 |
| Gene Sequence |
| Protein sequence |
