Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07455.t1 | GAU45563.1 | 82.9 | 152 | 25 | 1 | 1 | 151 | 1 | 152 | 5.20E-62 | 247.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07455.t1 | A0A2Z6NTR3 | 82.9 | 152 | 25 | 1 | 1 | 151 | 1 | 152 | 3.8e-62 | 247.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050361 | MS.gene07455 | 0.812359 | 4.41E-51 | -1.69E-46 |
| MS.gene050941 | MS.gene07455 | 0.800942 | 1.14E-48 | -1.69E-46 |
| MS.gene058081 | MS.gene07455 | 0.808928 | 2.43E-50 | -1.69E-46 |
| MS.gene060296 | MS.gene07455 | 0.813268 | 2.79E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07455.t1 | MTR_1g077090 | 97.419 | 155 | 4 | 0 | 1 | 155 | 1 | 155 | 5.38e-99 | 305 |
| MS.gene07455.t1 | MTR_6g065070 | 49.593 | 123 | 32 | 3 | 38 | 155 | 2 | 99 | 5.07e-25 | 102 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07455.t1 | AT5G08450 | 39.450 | 109 | 56 | 4 | 55 | 157 | 112 | 216 | 3.35e-12 | 65.9 |
| MS.gene07455.t1 | AT5G08450 | 39.450 | 109 | 56 | 4 | 55 | 157 | 112 | 216 | 3.35e-12 | 65.9 |
| MS.gene07455.t1 | AT5G08450 | 39.450 | 109 | 56 | 4 | 55 | 157 | 112 | 216 | 3.35e-12 | 65.9 |
Find 0 sgRNAs with CRISPR-Local
Find 77 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TATCATATGTCTTATGATAT+AGG | + | 8693:19303-19322 | MS.gene07455:CDS | 20.0% |
| ! | AAGAATCAGAAAAATACCAT+AGG | + | 8693:19712-19731 | MS.gene07455:intron | 25.0% |
| ! | AGAATCAGAAAAATACCATA+GGG | + | 8693:19713-19732 | MS.gene07455:intron | 25.0% |
| ! | AGAGAAAGATAGAAAAAGAA+AGG | + | 8693:19920-19939 | MS.gene07455:CDS | 25.0% |
| ! | GATAGTCATAATGATTCAAA+AGG | + | 8693:19630-19649 | MS.gene07455:CDS | 25.0% |
| !!! | AGAAAAGGATAGTTTTAATG+CGG | + | 8693:19665-19684 | MS.gene07455:intron | 25.0% |
| AAAATATATTCTGATCCTCC+TGG | + | 8693:19738-19757 | MS.gene07455:CDS | 30.0% | |
| TATGTCTTATGATATAGGTC+AGG | + | 8693:19308-19327 | MS.gene07455:CDS | 30.0% | |
| ! | GAGAACAAAATTGATTCCAA+AGG | + | 8693:19882-19901 | MS.gene07455:CDS | 30.0% |
| ! | TACTCATTTGAAACTGAAGA+CGG | - | 8693:19285-19304 | None:intergenic | 30.0% |
| !! | CAGAATATATTTTTGCCCTA+TGG | - | 8693:19731-19750 | None:intergenic | 30.0% |
| AGGTGATACTAAGACAGAAA+AGG | + | 8693:19650-19669 | MS.gene07455:intron | 35.0% | |
| ATGATATAGGTCAGGATTCT+CGG | + | 8693:19316-19335 | MS.gene07455:CDS | 35.0% | |
| ATTCTTTCCAATCCAAGTGA+AGG | - | 8693:19698-19717 | None:intergenic | 35.0% | |
| GACTCTTCATGAGATTTCTT+AGG | - | 8693:19204-19223 | None:intergenic | 35.0% | |
| GATGTTTGGAGAAGTAAGAA+AGG | + | 8693:19527-19546 | MS.gene07455:CDS | 35.0% | |
| TGAGAAGAGGTTAGAATCAA+GGG | + | 8693:19443-19462 | MS.gene07455:CDS | 35.0% | |
| TTTAGATCTATCGTCACCTT+TGG | - | 8693:19901-19920 | None:intergenic | 35.0% | |
| ! | TCAGAGTGCTAAAAGTGAAA+AGG | + | 8693:19554-19573 | MS.gene07455:CDS | 35.0% |
| !! | TTTTCACTTTTAGCACTCTG+AGG | - | 8693:19555-19574 | None:intergenic | 35.0% |
| ACAAGGATGGTAGACATGAT+CGG | + | 8693:19607-19626 | MS.gene07455:CDS | 40.0% | |
| ACTGAGAAGAAGGAGATGTT+TGG | + | 8693:19513-19532 | MS.gene07455:CDS | 40.0% | |
| AGTTAGAGGAGATGACAACA+AGG | + | 8693:19590-19609 | MS.gene07455:CDS | 40.0% | |
| ATCCGATACACTGAATGAAG+AGG | - | 8693:19378-19397 | None:intergenic | 40.0% | |
| ATGTTACGTGGGAATACACA+AGG | + | 8693:19783-19802 | MS.gene07455:CDS | 40.0% | |
| CCAAGGATAGTAGAGATATC+CGG | + | 8693:19469-19488 | MS.gene07455:CDS | 40.0% | |
| CTCCTCTTCATTCAGTGTAT+CGG | + | 8693:19373-19392 | MS.gene07455:CDS | 40.0% | |
| CTGAGAAGAGGTTAGAATCA+AGG | + | 8693:19442-19461 | MS.gene07455:CDS | 40.0% | |
| CTTGATTCTAACCTCTTCTC+AGG | - | 8693:19444-19463 | None:intergenic | 40.0% | |
| GAATACACAAGGTTCAGTTG+AGG | + | 8693:19794-19813 | MS.gene07455:CDS | 40.0% | |
| GATGCACATGTTGAAGTTAG+AGG | + | 8693:19576-19595 | MS.gene07455:CDS | 40.0% | |
| GTTAGAATCAAGGGAATCCA+AGG | + | 8693:19452-19471 | MS.gene07455:CDS | 40.0% | |
| TCGTATCACGATTCTCAAAC+CGG | - | 8693:19491-19510 | None:intergenic | 40.0% | |
| TGAGGATCACTAGAAGATGA+CGG | - | 8693:19402-19421 | None:intergenic | 40.0% | |
| TGATACGAAGACTGAGAAGA+AGG | + | 8693:19503-19522 | MS.gene07455:CDS | 40.0% | |
| TGATATAGGTCAGGATTCTC+GGG | + | 8693:19317-19336 | MS.gene07455:CDS | 40.0% | |
| TGTGTATTCCCACGTAACAT+GGG | - | 8693:19783-19802 | None:intergenic | 40.0% | |
| TTGTGTATTCCCACGTAACA+TGG | - | 8693:19784-19803 | None:intergenic | 40.0% | |
| !!! | GATAGTTTTAATGCGGCTAG+CGG | + | 8693:19672-19691 | MS.gene07455:intron | 40.0% |
| ACACAAGGTTCAGTTGAGGT+TGG | + | 8693:19798-19817 | MS.gene07455:CDS | 45.0% | |
| AGAGGAGATGACAACAAGGA+TGG | + | 8693:19594-19613 | MS.gene07455:CDS | 45.0% | |
| AGAGTTCATCAGCAGAACCA+AGG | + | 8693:19826-19845 | MS.gene07455:CDS | 45.0% | |
| AGGTTCAGTTGAGGTTGGAA+AGG | + | 8693:19803-19822 | MS.gene07455:CDS | 45.0% | |
| CAAGGATGGTAGACATGATC+GGG | + | 8693:19608-19627 | MS.gene07455:CDS | 45.0% | |
| CCGGATATCTCTACTATCCT+TGG | - | 8693:19472-19491 | None:intergenic | 45.0% | |
| GAGTTCATCAGCAGAACCAA+GGG | + | 8693:19827-19846 | MS.gene07455:CDS | 45.0% | |
| AACCTCTTCTCAGGACCAAC+AGG | - | 8693:19435-19454 | None:intergenic | 50.0% | |
| ACTGAAGACGGCGCCAATTT+CGG | - | 8693:19273-19292 | None:intergenic | 50.0% | |
| ATCCTGTTGGTCCTGAGAAG+AGG | + | 8693:19430-19449 | MS.gene07455:CDS | 50.0% | |
| CGCAGGTGCATATCCGAAAT+TGG | + | 8693:19257-19276 | MS.gene07455:CDS | 50.0% | |
| GATCTTCTCTCCACATCACG+AGG | - | 8693:19354-19373 | None:intergenic | 50.0% | |
| TATAGGTCAGGATTCTCGGG+TGG | + | 8693:19320-19339 | MS.gene07455:CDS | 50.0% | |
| TATGCACCTGCGTCTTCATG+CGG | - | 8693:19249-19268 | None:intergenic | 50.0% | |
| TCAGCAGAACCAAGGGAGTA+TGG | + | 8693:19834-19853 | MS.gene07455:CDS | 50.0% | |
| AAACACCCGCATGAAGACGC+AGG | + | 8693:19240-19259 | MS.gene07455:CDS | 55.0% | |
| AACCCTGGCCCATGTTACGT+GGG | + | 8693:19772-19791 | MS.gene07455:CDS | 55.0% | |
| ATGCACCTGCGTCTTCATGC+GGG | - | 8693:19248-19267 | None:intergenic | 55.0% | |
| ATTCCCACGTAACATGGGCC+AGG | - | 8693:19778-19797 | None:intergenic | 55.0% | |
| CTAGCGGCCTTCACTTGGAT+TGG | + | 8693:19688-19707 | MS.gene07455:intron | 55.0% | |
| CTCAGGACCAACAGGATGCT+CGG | - | 8693:19427-19446 | None:intergenic | 55.0% | |
| GGAAGCTCGTGAAGCTGTTG+GGG | + | 8693:19860-19879 | MS.gene07455:CDS | 55.0% | |
| GGGAAGCTCGTGAAGCTGTT+GGG | + | 8693:19859-19878 | MS.gene07455:CDS | 55.0% | |
| TTCCCACGTAACATGGGCCA+GGG | - | 8693:19777-19796 | None:intergenic | 55.0% | |
| ! | AGAACCAAGGGAGTATGGCG+GGG | + | 8693:19839-19858 | MS.gene07455:CDS | 55.0% |
| ! | CAGAACCAAGGGAGTATGGC+GGG | + | 8693:19838-19857 | MS.gene07455:CDS | 55.0% |
| ! | GCAGAACCAAGGGAGTATGG+CGG | + | 8693:19837-19856 | MS.gene07455:CDS | 55.0% |
| !! | GGTAAAAGCGCCTCGTGATG+TGG | + | 8693:19341-19360 | MS.gene07455:CDS | 55.0% |
| CCAACAGGATGCTCGGCATG+AGG | - | 8693:19420-19439 | None:intergenic | 60.0% | |
| CCTCATGCCGAGCATCCTGT+TGG | + | 8693:19417-19436 | MS.gene07455:CDS | 60.0% | |
| GAACCCTGGCCCATGTTACG+TGG | + | 8693:19771-19790 | MS.gene07455:CDS | 60.0% | |
| GGGGAAGCTCGTGAAGCTGT+TGG | + | 8693:19858-19877 | MS.gene07455:CDS | 60.0% | |
| TGATCCTCCTGGCGCGAGTT+TGG | + | 8693:19749-19768 | MS.gene07455:CDS | 60.0% | |
| CAGGGTTCCAAACTCGCGCC+AGG | - | 8693:19759-19778 | None:intergenic | 65.0% | |
| GCTTCCCCGCCATACTCCCT+TGG | - | 8693:19846-19865 | None:intergenic | 65.0% | |
| GGTTCCAAACTCGCGCCAGG+AGG | - | 8693:19756-19775 | None:intergenic | 65.0% | |
| TGCGGCTAGCGGCCTTCACT+TGG | + | 8693:19683-19702 | MS.gene07455:intron | 65.0% | |
| ! | CTGGCGCGAGTTTGGAACCC+TGG | + | 8693:19757-19776 | MS.gene07455:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 8693 | gene | 19189 | 19948 | 19189 | ID=MS.gene07455 |
| 8693 | mRNA | 19189 | 19948 | 19189 | ID=MS.gene07455.t1;Parent=MS.gene07455 |
| 8693 | exon | 19189 | 19651 | 19189 | ID=MS.gene07455.t1.exon1;Parent=MS.gene07455.t1 |
| 8693 | CDS | 19189 | 19651 | 19189 | ID=cds.MS.gene07455.t1;Parent=MS.gene07455.t1 |
| 8693 | exon | 19734 | 19948 | 19734 | ID=MS.gene07455.t1.exon2;Parent=MS.gene07455.t1 |
| 8693 | CDS | 19734 | 19948 | 19734 | ID=cds.MS.gene07455.t1;Parent=MS.gene07455.t1 |
| Gene Sequence |
| Protein sequence |
