AlfalfaGEDB | MS.gene050361

M. sativa cultivar XinJiangDaYe / MS.gene050361

KEGG: -
GO: GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005575 GO:0005634 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0097159 GO:0110165 GO:1901363
PFAM: PF03110.17

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene050361.t1	XP_003591325.1	95.7	253	11	0	1	253	1	253	4.00E-135	490.7

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene050361.t1	Q9SMX9	57.3	248	62	8	6	253	8	211	5.4e-64	245.7

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene050361.t1	G7IBS5	95.7	253	11	0	1	253	1	253	2.9e-135	490.7

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TFs/TRs:

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Gene ID	Type	Classification
MS.gene050361.t1	TF	SBP

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050084	MS.gene050361	0.801399	9.17E-49	-1.69E-46
MS.gene050085	MS.gene050361	0.811253	7.68E-51	-1.69E-46
MS.gene050361	MS.gene050646	0.811319	7.43E-51	-1.69E-46
MS.gene050361	MS.gene050654	0.812235	4.69E-51	-1.69E-46
MS.gene050361	MS.gene050762	0.806855	6.72E-50	-1.69E-46
MS.gene050361	MS.gene050763	0.803819	2.91E-49	-1.69E-46
MS.gene050361	MS.gene051631	-0.809595	1.75E-50	-1.69E-46
MS.gene050361	MS.gene051856	0.806782	6.96E-50	-1.69E-46
MS.gene050361	MS.gene052242	-0.804157	2.47E-49	-1.69E-46
MS.gene050361	MS.gene052609	0.803332	3.67E-49	-1.69E-46

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PPI:

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene050361.t1	MTR_1g086250	95.652	253	11	0	1	253	1	253	7.98e-158	464
MS.gene050361.t1	MTR_7g110320	65.041	246	64	8	8	240	2	238	4.68e-91	286
MS.gene050361.t1	MTR_7g110320	66.409	259	65	8	8	253	2	251	2.36e-90	287
MS.gene050361.t1	MTR_2g046550	54.826	259	98	7	1	253	1	246	5.28e-80	259
MS.gene050361.t1	MTR_1g035010	56.115	139	56	3	115	251	74	209	3.18e-41	150
MS.gene050361.t1	MTR_2g078770	62.376	101	38	0	132	232	43	143	1.82e-36	127
MS.gene050361.t1	MTR_8g463140	70.588	85	25	0	146	230	64	148	1.34e-35	125
MS.gene050361.t1	MTR_8g005960	42.051	195	90	6	47	221	70	261	5.42e-35	127
MS.gene050361.t1	MTR_8g096780	58.511	94	39	0	133	226	85	178	1.43e-33	125
MS.gene050361.t1	MTR_4g088555	64.368	87	31	0	135	221	51	137	1.88e-33	119

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene050361.t1	AT2G47070	57.258	248	62	8	6	253	8	211	8.78e-74	241
MS.gene050361.t1	AT3G60030	53.815	249	78	7	16	253	11	233	6.73e-73	239
MS.gene050361.t1	AT1G20980	58.915	129	45	2	130	250	103	231	3.61e-42	153
MS.gene050361.t1	AT1G76580	39.224	232	82	7	30	252	40	221	2.84e-39	144
MS.gene050361.t1	AT1G76580	39.224	232	82	7	30	252	40	221	3.30e-39	144
MS.gene050361.t1	AT1G76580	39.224	232	82	7	30	252	40	221	9.91e-39	143
MS.gene050361.t1	AT2G47070	78.313	83	17	1	171	253	1	82	1.09e-36	137
MS.gene050361.t1	AT2G33810	65.060	83	29	0	140	222	47	129	6.91e-35	122
MS.gene050361.t1	AT1G69170	61.458	96	37	0	132	227	109	204	1.16e-34	128
MS.gene050361.t1	AT1G02065	69.620	79	24	0	147	225	188	266	1.76e-33	124

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Find 73 sgRNAs with CRISPR-Local

Find 179 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAGGCTAGTAGGGCTCTTGT+GGG	0.562216	1.2:+64975194	MS.gene050361:CDS
AAGGGAATAAGGAAGGGGAT+AGG	0.469713	1.2:+64974943	MS.gene050361:CDS
AATCAAGAAGCTGTTCCTAA+TGG	0.397811	1.2:+64976654	MS.gene050361:CDS
ACCGGGATCCCAGACCGGAG+TGG	0.666079	1.2:-64974864	None:intergenic
ACTCCGGTCTGGGATCCCGG+TGG	0.685385	1.2:+64974866	MS.gene050361:CDS
AGATCTTGAGAAAGGGAATA+AGG	0.299596	1.2:+64974932	MS.gene050361:CDS
AGATGCATTCTAAGGCTAGT+AGG	0.412881	1.2:+64975183	MS.gene050361:CDS
AGCTGTCGAAGACGCTTGGC+TGG	0.450145	1.2:+64976606	MS.gene050361:CDS
AGGAAGGAGATCTTGAGAAA+GGG	0.547901	1.2:+64974925	MS.gene050361:CDS
AGGACATGTTTCACCGGCTG+TGG	0.490256	1.2:+64975037	MS.gene050361:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAATTGGGTTTGTCTAACT+GGG	+	chr1.2:64975646-64975665	MS.gene050361:intron	30.0%
AAACGTTAGATAATTTGGGA+CGG	-	chr1.2:64975911-64975930	None:intergenic	30.0%
AACAGAAAGCAATCAATAAG+AGG	-	chr1.2:64975267-64975286	None:intergenic	30.0%
AATACTGCCTAATATCATGA+TGG	+	chr1.2:64976407-64976426	MS.gene050361:intron	30.0%
ATTAAAGGATAACAAGGACT+TGG	-	chr1.2:64975807-64975826	None:intergenic	30.0%
ATTGCCCTTAATAAATGGTA+TGG	+	chr1.2:64975985-64976004	MS.gene050361:intron	30.0%
CTGAAAATCTATGTTGTTGA+TGG	+	chr1.2:64975371-64975390	MS.gene050361:intron	30.0%
GAATTTGAATGATTGGAGAT+GGG	+	chr1.2:64974767-64974786	MS.gene050361:CDS	30.0%
GCCAAAACGTTAGATAATTT+GGG	-	chr1.2:64975915-64975934	None:intergenic	30.0%
TAAAATATGCTACAGCAGTA+TGG	-	chr1.2:64976323-64976342	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1.2	gene	64974675	64976750	64974675	ID=MS.gene050361
chr1.2	mRNA	64974675	64976750	64974675	ID=MS.gene050361.t1;Parent=MS.gene050361
chr1.2	exon	64974675	64975252	64974675	ID=MS.gene050361.t1.exon1;Parent=MS.gene050361.t1
chr1.2	CDS	64974675	64975252	64974675	ID=cds.MS.gene050361.t1;Parent=MS.gene050361.t1
chr1.2	exon	64976569	64976750	64976569	ID=MS.gene050361.t1.exon2;Parent=MS.gene050361.t1
chr1.2	CDS	64976569	64976750	64976569	ID=cds.MS.gene050361.t1;Parent=MS.gene050361.t1

Gene Sequence

>MS.gene050361

ATGGGGACAAGGTTGGGTGCTGAAAATTATCATTTTTATGGTGTTGGTGGTTCATCTGATTTGAGTGGGATGGGGAGGAGGTCAAGGGAATGGAATTTGAATGATTGGAGATGGGATGGTGATTTGTTTATAGCTAGCCGTGTGAATCCGGTGCAGGCGGACAGTTTGCGAGTGGGACAACAGTTTTTCCCACTCCGGTCTGGGATCCCGGTGGTTGGAGGTTCGTCCAATACGTCTTCTTCATGCTCCGAGGAAGGAGATCTTGAGAAAGGGAATAAGGAAGGGGATAGGAAAAGGAGAGTTATTGTGCTGGAAGATGATGGTTTGAATGATGAAGCTGGTGCACTTAGCTTAAATCTTGCAGGACATGTTTCACCGGCTGTGGAAAGGGACGGAAAAAAGAGCAGAGTAGCTGGAGGAACTTCGAATCGAGCTGTCTGTCAGGTTGAAGACTGTGGTGCAGATCTGAGTAGAGGTAAGGATTATCACAGACGTCATAAAGTTTGTGAGATGCATTCTAAGGCTAGTAGGGCTCTTGTGGGAAATGCAATGCAGAGGTTTTGTCAACAGTGTAGTAGGTAATTAATTTCCTCTTATTGATTGCTTTCTGTTTTTGCTTTGGTTGATAGTTATGTGTGTATAGGAAATAAATGCTATTGTTGTTAATCAATTACCGCTCCAAAATGTAATCAATATCTGAAAATCTATGTTGTTGATGGCGCCGCGATAGCGGAATAGTGTAGTGGGTCAGGGTTTGAAGAAATGGCCTCGCTCTCTTGCACACGACATGATTCCTCCCCCTCCCCGCGCCTGCAATGTGCCATTGTTCGAAAGTTTGCCATGTGAGATCTCTCTATATAATTAATTGATCTCATTGCACATGCAACACCGACATACAAACTATGCTCGCTATTGAATAATCAGTGTAAAATTAGGGCTTTTACCTGTCATAGCCTACGCATCCCTGGTGCAAAATTGGGTTTGTCTAACTGGGCCAAACATGTGCAGTTCCTCTCCCAATTTTCCTGAGGAGGGTTATTGGCCTCACGACCCACAGCCCAAACTCTTTCGTTTTTTTAATTAAATTAATTTAAGCGAGTGAAAAAAACTAAATTTAATTTAATTCTGTCCAAGTCCTTGTTATCCTTTAATTTTTTAATTCTAATTATATTTAGATTACATTTATAACCTGATTTTGATGGGAATGGACAAAGTTAACTATAATTACTCCCTCCGTCCCAAATTATCTAACGTTTTGGCCATGTAATTAATTTTATATGCGAAAGAGATATAATGAGTTGTTTTACAAAATTGCCCTTAATAAATGGTATGGCAAAAAAATCAATTAAAGAATTGAAAGAAGAGAGAGTAATAAATAGTTAAGGATATAATAGGAAAATTAACATTAATGTTTCATTGGTATTGTAAAGCGACATATAATTTGGGACAATTTTTTTTTCAAAGCGACATATAATTGGGACGGAGGGAGTATTAAGTATGATTTTTTTAAGGCAAATTATTAAGTATGATCGTCTCTTTAATTTTGTTGTACTTTTCTACTTTTGAGTATTTATTGTATCTTGTTGGAATTTGTGTTTACTTTGTACACTGTTAGTCTTCGCAACAGCGGTTATCCGGCTATCCGCCATACTGCTGTAGCATATTTTAGGTTTGGTGCTATGGGCTGCCATCTGTTATTAACAAGTTAGCTGAAAATTGAAAACTATATCTCAATACTGCCTAATATCATGATGGATTTTTATTAGTACTCCTTTATTCAATAATTTTGTATTAATTTTTATGTAGGATTCTCTCAAAAAAAAATAAATTTTTTTTATGCAAGTGTTTGATGTAAATAACACTATTGCATGACATTCTTTTACCCCATCAACAGGTTTCACATACTTCAAGAGTTCGATGAAGGAAAGAGAAGCTGTCGAAGACGCTTGGCTGGCCATAACAAACGGAGAAGGAAAACAAATCAAGAAGCTGTTCCTAATGGAAGTCCAACAAATGATGATCAGACTAGTAGTTATCTGTTGATTAGTTTATTGAAGATACTTTCAAATATGCATT

Protein sequence

>MS.gene050361.t1

MGTRLGAENYHFYGVGGSSDLSGMGRRSREWNLNDWRWDGDLFIASRVNPVQADSLRVGQQFFPLRSGIPVVGGSSNTSSSCSEEGDLEKGNKEGDRKRRVIVLEDDGLNDEAGALSLNLAGHVSPAVERDGKKSRVAGGTSNRAVCQVEDCGADLSRGKDYHRRHKVCEMHSKASRALVGNAMQRFCQQCSRFHILQEFDEGKRSCRRRLAGHNKRRRKTNQEAVPNGSPTNDDQTSSYLLISLLKILSNMH