Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07641.t1 | XP_013451990.1 | 89.1 | 119 | 13 | 0 | 1 | 119 | 1 | 119 | 5.60E-55 | 223.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07641.t1 | G7ZYN0 | 89.1 | 119 | 13 | 0 | 1 | 119 | 1 | 119 | 4.0e-55 | 223.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050381 | MS.gene07641 | 0.801809 | 7.56E-49 | -1.69E-46 |
| MS.gene053420 | MS.gene07641 | 0.800258 | 1.57E-48 | -1.69E-46 |
| MS.gene058171 | MS.gene07641 | 0.807823 | 4.19E-50 | -1.69E-46 |
| MS.gene058173 | MS.gene07641 | 0.806531 | 7.86E-50 | -1.69E-46 |
| MS.gene058175 | MS.gene07641 | 0.803934 | 2.75E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07641.t1 | MTR_6g039830 | 89.076 | 119 | 13 | 0 | 1 | 119 | 1 | 119 | 4.57e-77 | 223 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07641.t1 | AT1G71740 | 52.212 | 113 | 39 | 4 | 22 | 119 | 18 | 130 | 9.30e-31 | 106 |
| MS.gene07641.t1 | AT1G49000 | 53.061 | 49 | 17 | 1 | 72 | 114 | 102 | 150 | 8.93e-11 | 56.2 |
Find 26 sgRNAs with CRISPR-Local
Find 25 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTGGCAGAAGAATATACTTA+TGG | 0.292425 | 6.1:-22416820 | MS.gene07641:CDS |
| TCACTAAGCACATCAAATAT+TGG | 0.311867 | 6.1:-22417020 | MS.gene07641:CDS |
| TCATTGTTTAGCTCTATAAC+AGG | 0.379640 | 6.1:+22416891 | None:intergenic |
| AACAAGAAGAGGATTCGATT+TGG | 0.401052 | 6.1:-22416839 | MS.gene07641:CDS |
| TGTCAGCTTCCTGATTTCTC+TGG | 0.417988 | 6.1:-22416789 | MS.gene07641:CDS |
| TGGCAGAAGAATATACTTAT+GGG | 0.423192 | 6.1:-22416819 | MS.gene07641:CDS |
| GTGCTTCTCATTCGCGCAAA+TGG | 0.423947 | 6.1:-22416950 | MS.gene07641:CDS |
| ATTCGCGCAAATGGCGCCAT+TGG | 0.444761 | 6.1:-22416941 | MS.gene07641:CDS |
| GCTCACTTGCAACGCATTTG+CGG | 0.465518 | 6.1:-22416919 | MS.gene07641:CDS |
| ACGCAACGGCACACATCATA+AGG | 0.467823 | 6.1:+22416982 | None:intergenic |
| CCCTACTTCCATGTTAGTAA+TGG | 0.489206 | 6.1:+22416711 | None:intergenic |
| TTGATGTGCTTAGTGAATGT+TGG | 0.502194 | 6.1:+22417027 | None:intergenic |
| ATGCGTTGCAAGTGAGCCAA+TGG | 0.513591 | 6.1:+22416925 | None:intergenic |
| AATGGAAGAGAAGTTCTAGC+AGG | 0.517247 | 6.1:+22416729 | None:intergenic |
| ATTATGACACCAGAGAAATC+AGG | 0.521571 | 6.1:+22416780 | None:intergenic |
| CTTCTCTTCCATTACTAACA+TGG | 0.528632 | 6.1:-22416719 | MS.gene07641:CDS |
| TCCATTACTAACATGGAAGT+AGG | 0.540252 | 6.1:-22416712 | None:intergenic |
| TAGAGAAGAAGAACAAGAAG+AGG | 0.545056 | 6.1:-22416850 | MS.gene07641:CDS |
| CACTTGCAACGCATTTGCGG+AGG | 0.564076 | 6.1:-22416916 | MS.gene07641:CDS |
| CCATTACTAACATGGAAGTA+GGG | 0.570236 | 6.1:-22416711 | None:intergenic |
| CAGAAGAATATACTTATGGG+TGG | 0.597191 | 6.1:-22416816 | MS.gene07641:CDS |
| ATGGAAGAGAAGTTCTAGCA+GGG | 0.600945 | 6.1:+22416730 | None:intergenic |
| GTCATAATCTATGATTCTGA+TGG | 0.631979 | 6.1:-22416765 | MS.gene07641:CDS |
| AGCACACATGAGCAACGCAA+CGG | 0.634273 | 6.1:+22416968 | None:intergenic |
| AGAAGAATATACTTATGGGT+GGG | 0.641405 | 6.1:-22416815 | MS.gene07641:CDS |
| AATGAAGTTGTGATGATCAG+TGG | 0.678528 | 6.1:-22416873 | MS.gene07641:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AGAAGAATATACTTATGGGT+GGG | - | chr6.1:22416948-22416967 | MS.gene07641:CDS | 30.0% | |
| GTCATAATCTATGATTCTGA+TGG | - | chr6.1:22416998-22417017 | MS.gene07641:CDS | 30.0% | |
| TCACTAAGCACATCAAATAT+TGG | - | chr6.1:22416743-22416762 | MS.gene07641:CDS | 30.0% | |
| TGGCAGAAGAATATACTTAT+GGG | - | chr6.1:22416944-22416963 | MS.gene07641:CDS | 30.0% | |
| TTGGCAGAAGAATATACTTA+TGG | - | chr6.1:22416943-22416962 | MS.gene07641:CDS | 30.0% | |
| !! | TCATTGTTTAGCTCTATAAC+AGG | + | chr6.1:22416875-22416894 | None:intergenic | 30.0% |
| !!! | ACATCAAATATTGGTTTTGC+TGG | - | chr6.1:22416752-22416771 | MS.gene07641:CDS | 30.0% |
| AACAAGAAGAGGATTCGATT+TGG | - | chr6.1:22416924-22416943 | MS.gene07641:CDS | 35.0% | |
| AATGAAGTTGTGATGATCAG+TGG | - | chr6.1:22416890-22416909 | MS.gene07641:CDS | 35.0% | |
| ATTATGACACCAGAGAAATC+AGG | + | chr6.1:22416986-22417005 | None:intergenic | 35.0% | |
| CAGAAGAATATACTTATGGG+TGG | - | chr6.1:22416947-22416966 | MS.gene07641:CDS | 35.0% | |
| CTTCTCTTCCATTACTAACA+TGG | - | chr6.1:22417044-22417063 | MS.gene07641:CDS | 35.0% | |
| GAATGTTGGATCACAAAAAG+TGG | + | chr6.1:22416725-22416744 | None:intergenic | 35.0% | |
| TAGAGAAGAAGAACAAGAAG+AGG | - | chr6.1:22416913-22416932 | MS.gene07641:CDS | 35.0% | |
| TTGATGTGCTTAGTGAATGT+TGG | + | chr6.1:22416739-22416758 | None:intergenic | 35.0% | |
| ! | AATGGAAGAGAAGTTCTAGC+AGG | + | chr6.1:22417037-22417056 | None:intergenic | 40.0% |
| ! | ATGGAAGAGAAGTTCTAGCA+GGG | + | chr6.1:22417036-22417055 | None:intergenic | 40.0% |
| TGTCAGCTTCCTGATTTCTC+TGG | - | chr6.1:22416974-22416993 | MS.gene07641:CDS | 45.0% | |
| ACGCAACGGCACACATCATA+AGG | + | chr6.1:22416784-22416803 | None:intergenic | 50.0% | |
| AGCACACATGAGCAACGCAA+CGG | + | chr6.1:22416798-22416817 | None:intergenic | 50.0% | |
| ATGCGTTGCAAGTGAGCCAA+TGG | + | chr6.1:22416841-22416860 | None:intergenic | 50.0% | |
| GCTCACTTGCAACGCATTTG+CGG | - | chr6.1:22416844-22416863 | MS.gene07641:CDS | 50.0% | |
| GTGCTTCTCATTCGCGCAAA+TGG | - | chr6.1:22416813-22416832 | MS.gene07641:CDS | 50.0% | |
| ATTCGCGCAAATGGCGCCAT+TGG | - | chr6.1:22416822-22416841 | MS.gene07641:CDS | 55.0% | |
| CACTTGCAACGCATTTGCGG+AGG | - | chr6.1:22416847-22416866 | MS.gene07641:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.1 | gene | 22416713 | 22417072 | 22416713 | ID=MS.gene07641 |
| chr6.1 | mRNA | 22416713 | 22417072 | 22416713 | ID=MS.gene07641.t1;Parent=MS.gene07641 |
| chr6.1 | exon | 22416713 | 22417072 | 22416713 | ID=MS.gene07641.t1.exon1;Parent=MS.gene07641.t1 |
| chr6.1 | CDS | 22416713 | 22417072 | 22416713 | ID=cds.MS.gene07641.t1;Parent=MS.gene07641.t1 |
| Gene Sequence |
| Protein sequence |
