Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07781.t1 | XP_003627109.1 | 89.5 | 524 | 40 | 5 | 1 | 516 | 1 | 517 | 5.00E-217 | 763.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07781.t1 | Q8H1F5 | 62.9 | 334 | 99 | 6 | 18 | 349 | 21 | 331 | 3.2e-95 | 350.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07781.t1 | G7LHG9 | 89.5 | 524 | 40 | 5 | 1 | 516 | 1 | 517 | 3.6e-217 | 763.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene07781.t1 | TF | C2H2 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052723 | MS.gene07781 | 0.872625 | 2.75E-67 | -1.69E-46 |
| MS.gene052725 | MS.gene07781 | 0.802225 | 6.21E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07781.t1 | MTR_8g017210 | 91.221 | 524 | 31 | 5 | 1 | 516 | 1 | 517 | 0.0 | 863 |
| MS.gene07781.t1 | MTR_2g099990 | 57.985 | 526 | 135 | 22 | 18 | 516 | 1 | 467 | 0.0 | 517 |
| MS.gene07781.t1 | MTR_2g099990 | 57.395 | 453 | 118 | 18 | 84 | 516 | 1 | 398 | 2.35e-155 | 449 |
| MS.gene07781.t1 | MTR_1g016010 | 47.784 | 519 | 190 | 15 | 1 | 510 | 1 | 447 | 4.47e-133 | 394 |
| MS.gene07781.t1 | MTR_4g059870 | 45.243 | 515 | 211 | 15 | 29 | 506 | 8 | 488 | 7.84e-119 | 358 |
| MS.gene07781.t1 | MTR_1g094115 | 88.415 | 164 | 19 | 0 | 58 | 221 | 45 | 208 | 9.44e-111 | 339 |
| MS.gene07781.t1 | MTR_1g112270 | 87.500 | 160 | 20 | 0 | 63 | 222 | 47 | 206 | 3.14e-107 | 331 |
| MS.gene07781.t1 | MTR_2g093960 | 68.981 | 216 | 56 | 4 | 59 | 265 | 31 | 244 | 1.24e-104 | 320 |
| MS.gene07781.t1 | MTR_1g112270 | 82.738 | 168 | 29 | 0 | 55 | 222 | 50 | 217 | 1.10e-103 | 323 |
| MS.gene07781.t1 | MTR_2g090745 | 50.992 | 353 | 131 | 8 | 70 | 385 | 17 | 364 | 1.73e-101 | 312 |
| MS.gene07781.t1 | MTR_1g094115 | 79.878 | 164 | 16 | 1 | 58 | 221 | 45 | 191 | 7.26e-95 | 298 |
| MS.gene07781.t1 | MTR_2g017720 | 76.667 | 120 | 23 | 1 | 65 | 184 | 318 | 432 | 4.57e-57 | 196 |
| MS.gene07781.t1 | MTR_3g102000 | 71.717 | 99 | 21 | 2 | 87 | 181 | 36 | 131 | 5.37e-41 | 147 |
| MS.gene07781.t1 | MTR_5g030560 | 72.000 | 50 | 14 | 0 | 65 | 114 | 11 | 60 | 2.36e-20 | 85.9 |
| MS.gene07781.t1 | MTR_1g093095 | 33.333 | 156 | 89 | 6 | 83 | 225 | 109 | 262 | 1.72e-19 | 89.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07781.t1 | AT3G13810 | 48.007 | 552 | 182 | 17 | 15 | 516 | 17 | 513 | 6.43e-142 | 419 |
| MS.gene07781.t1 | AT3G13810 | 48.007 | 552 | 182 | 17 | 15 | 516 | 17 | 513 | 6.43e-142 | 419 |
| MS.gene07781.t1 | AT3G13810 | 48.007 | 552 | 182 | 17 | 15 | 516 | 12 | 508 | 6.51e-142 | 418 |
| MS.gene07781.t1 | AT3G13810 | 47.905 | 549 | 181 | 17 | 18 | 516 | 1 | 494 | 2.34e-140 | 414 |
| MS.gene07781.t1 | AT1G55110 | 49.390 | 492 | 177 | 14 | 14 | 501 | 17 | 440 | 5.71e-134 | 396 |
| MS.gene07781.t1 | AT1G55110 | 49.390 | 492 | 177 | 14 | 14 | 501 | 17 | 440 | 5.71e-134 | 396 |
| MS.gene07781.t1 | AT1G55110 | 49.390 | 492 | 177 | 14 | 14 | 501 | 17 | 440 | 5.71e-134 | 396 |
| MS.gene07781.t1 | AT3G13810 | 45.699 | 558 | 192 | 17 | 15 | 516 | 12 | 514 | 4.63e-128 | 383 |
| MS.gene07781.t1 | AT3G13810 | 45.586 | 555 | 191 | 17 | 18 | 516 | 1 | 500 | 1.69e-126 | 379 |
| MS.gene07781.t1 | AT5G44160 | 49.015 | 406 | 169 | 8 | 29 | 400 | 8 | 409 | 2.04e-112 | 341 |
| MS.gene07781.t1 | AT5G66730 | 75.362 | 207 | 34 | 2 | 17 | 222 | 4 | 194 | 5.28e-112 | 342 |
| MS.gene07781.t1 | AT3G50700 | 72.857 | 210 | 38 | 2 | 17 | 223 | 4 | 197 | 1.16e-111 | 339 |
| MS.gene07781.t1 | AT4G02670 | 52.243 | 379 | 107 | 12 | 27 | 399 | 17 | 327 | 2.00e-109 | 332 |
| MS.gene07781.t1 | AT4G02670 | 52.243 | 379 | 107 | 12 | 27 | 399 | 19 | 329 | 2.02e-109 | 332 |
| MS.gene07781.t1 | AT1G14580 | 72.277 | 202 | 52 | 1 | 21 | 222 | 18 | 215 | 6.56e-109 | 332 |
| MS.gene07781.t1 | AT1G14580 | 72.277 | 202 | 52 | 1 | 21 | 222 | 18 | 215 | 6.56e-109 | 332 |
| MS.gene07781.t1 | AT3G45260 | 81.768 | 181 | 24 | 2 | 60 | 231 | 39 | 219 | 8.80e-109 | 331 |
| MS.gene07781.t1 | AT3G45260 | 81.768 | 181 | 24 | 2 | 60 | 231 | 39 | 219 | 8.80e-109 | 331 |
| MS.gene07781.t1 | AT1G14580 | 72.277 | 202 | 52 | 1 | 21 | 222 | 32 | 229 | 1.78e-108 | 332 |
| MS.gene07781.t1 | AT2G02070 | 79.888 | 179 | 34 | 1 | 60 | 238 | 52 | 228 | 9.70e-106 | 328 |
| MS.gene07781.t1 | AT2G02070 | 79.888 | 179 | 34 | 1 | 60 | 238 | 52 | 228 | 9.70e-106 | 328 |
| MS.gene07781.t1 | AT5G03150 | 74.129 | 201 | 40 | 3 | 60 | 257 | 53 | 244 | 5.74e-105 | 323 |
| MS.gene07781.t1 | AT5G44160 | 49.589 | 365 | 148 | 7 | 70 | 400 | 8 | 370 | 3.03e-103 | 317 |
| MS.gene07781.t1 | AT5G60470 | 78.947 | 171 | 35 | 1 | 56 | 225 | 40 | 210 | 1.63e-102 | 316 |
| MS.gene07781.t1 | AT5G60470 | 78.947 | 171 | 35 | 1 | 56 | 225 | 36 | 206 | 1.98e-102 | 315 |
| MS.gene07781.t1 | AT2G02080 | 43.319 | 464 | 181 | 12 | 58 | 466 | 52 | 488 | 2.59e-102 | 317 |
| MS.gene07781.t1 | AT2G02080 | 43.319 | 464 | 181 | 12 | 58 | 466 | 52 | 488 | 2.59e-102 | 317 |
| MS.gene07781.t1 | AT2G02080 | 43.319 | 464 | 181 | 12 | 58 | 466 | 52 | 488 | 2.59e-102 | 317 |
| MS.gene07781.t1 | AT2G02080 | 43.319 | 464 | 181 | 12 | 58 | 466 | 52 | 488 | 2.59e-102 | 317 |
| MS.gene07781.t1 | AT5G60470 | 73.988 | 173 | 43 | 2 | 52 | 222 | 26 | 198 | 3.72e-94 | 294 |
| MS.gene07781.t1 | AT2G02080 | 41.553 | 438 | 174 | 12 | 84 | 466 | 1 | 411 | 4.31e-87 | 275 |
| MS.gene07781.t1 | AT2G02080 | 41.553 | 438 | 174 | 12 | 84 | 466 | 1 | 411 | 4.31e-87 | 275 |
| MS.gene07781.t1 | AT1G25250 | 65.605 | 157 | 51 | 2 | 60 | 213 | 33 | 189 | 5.18e-74 | 239 |
| MS.gene07781.t1 | AT1G68130 | 65.385 | 156 | 52 | 2 | 60 | 213 | 41 | 196 | 1.78e-73 | 239 |
| MS.gene07781.t1 | AT2G01940 | 64.198 | 162 | 56 | 2 | 56 | 215 | 40 | 201 | 3.07e-73 | 239 |
| MS.gene07781.t1 | AT1G25250 | 62.577 | 163 | 56 | 3 | 56 | 213 | 4 | 166 | 5.22e-71 | 231 |
| MS.gene07781.t1 | AT2G01940 | 63.190 | 163 | 57 | 3 | 56 | 215 | 40 | 202 | 1.04e-69 | 230 |
| MS.gene07781.t1 | AT1G68130 | 66.364 | 110 | 35 | 2 | 106 | 213 | 1 | 110 | 3.58e-47 | 167 |
| MS.gene07781.t1 | AT1G25250 | 65.766 | 111 | 35 | 2 | 106 | 213 | 1 | 111 | 9.75e-47 | 165 |
| MS.gene07781.t1 | AT2G01940 | 64.286 | 112 | 38 | 2 | 106 | 215 | 1 | 112 | 8.02e-46 | 164 |
| MS.gene07781.t1 | AT1G34370 | 33.544 | 158 | 91 | 3 | 69 | 212 | 14 | 171 | 7.92e-24 | 102 |
| MS.gene07781.t1 | AT1G34370 | 33.544 | 158 | 91 | 3 | 69 | 212 | 224 | 381 | 1.26e-23 | 104 |
| MS.gene07781.t1 | AT1G34370 | 33.544 | 158 | 91 | 3 | 69 | 212 | 224 | 381 | 1.26e-23 | 104 |
| MS.gene07781.t1 | AT5G22890 | 33.735 | 166 | 94 | 5 | 61 | 212 | 191 | 354 | 1.80e-23 | 102 |
| MS.gene07781.t1 | AT1G13290 | 34.437 | 151 | 85 | 6 | 83 | 221 | 95 | 243 | 1.81e-19 | 89.4 |
| MS.gene07781.t1 | AT1G08290 | 28.509 | 228 | 137 | 9 | 9 | 212 | 87 | 312 | 1.42e-18 | 87.4 |
| MS.gene07781.t1 | AT1G51220 | 33.094 | 139 | 83 | 4 | 83 | 212 | 172 | 309 | 4.51e-18 | 85.9 |
| MS.gene07781.t1 | AT3G20880 | 31.447 | 159 | 93 | 5 | 83 | 226 | 250 | 407 | 5.19e-18 | 86.7 |
| MS.gene07781.t1 | AT3G57670 | 33.571 | 140 | 81 | 5 | 83 | 212 | 211 | 348 | 7.55e-18 | 85.9 |
| MS.gene07781.t1 | AT1G34790 | 33.557 | 149 | 89 | 4 | 73 | 212 | 129 | 276 | 6.96e-17 | 82.0 |
Find 97 sgRNAs with CRISPR-Local
Find 138 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTCTTCTCACCATGCTTTC+TGG | 0.052883 | 8.1:-80684183 | None:intergenic |
| CACCAAACATTTCATCAAAT+TGG | 0.116876 | 8.1:-80685637 | None:intergenic |
| TTCTCTAGTGCCACATGTTT+TGG | 0.232714 | 8.1:-80684264 | None:intergenic |
| TGTAATCATAAGATGGTAAA+AGG | 0.239938 | 8.1:+80683610 | None:intergenic |
| CCTAGTCTTGTTCATGTTCC+TGG | 0.244428 | 8.1:+80685299 | MS.gene07781:CDS |
| TCTGGTAACAGAACTGAAAT+TGG | 0.252456 | 8.1:+80683715 | MS.gene07781:CDS |
| GACGGTGGCGGCGGCGGCGG+CGG | 0.256319 | 8.1:+80685728 | MS.gene07781:CDS |
| GCCGCCGCCGCCACCGTCAT+TGG | 0.303592 | 8.1:-80685723 | None:intergenic |
| CTTGTTGAGGATTATTGAAT+TGG | 0.313076 | 8.1:-80685100 | None:intergenic |
| CATGATCCATCAAGAGCATT+AGG | 0.324615 | 8.1:+80684131 | MS.gene07781:CDS |
| TGTTGTTGTGAGGGTAAAAT+TGG | 0.350354 | 8.1:-80685032 | None:intergenic |
| AGGTTTGACAAGAGATTTCT+TGG | 0.358914 | 8.1:+80685763 | MS.gene07781:CDS |
| AATGACGGTGGCGGCGGCGG+CGG | 0.359203 | 8.1:+80685725 | MS.gene07781:CDS |
| CAAGAGATTTCTTGGGGCTT+AGG | 0.363505 | 8.1:+80685771 | MS.gene07781:CDS |
| ACCAAACATTTCATCAAATT+GGG | 0.370739 | 8.1:-80685636 | None:intergenic |
| ATGATCCATCAAGAGCATTA+GGG | 0.372273 | 8.1:+80684132 | MS.gene07781:CDS |
| ATAGCTATAACCTCAGCATC+AGG | 0.391905 | 8.1:-80683924 | None:intergenic |
| AATAATGTCTCTGGTAACTT+TGG | 0.395509 | 8.1:+80685476 | MS.gene07781:CDS |
| CTTCCACTACTTGTTGCTGT+TGG | 0.402652 | 8.1:-80683645 | None:intergenic |
| GGTGAAGCTAGTGTTTCTTC+TGG | 0.407702 | 8.1:+80683697 | MS.gene07781:CDS |
| AAATCCCCTAATGCTCTTGA+TGG | 0.408372 | 8.1:-80684137 | None:intergenic |
| GTTGCAGAAAGCAGCTCAAA+TGG | 0.410516 | 8.1:+80685379 | MS.gene07781:CDS |
| TCTGAAATTCCACCTTGGCT+TGG | 0.415974 | 8.1:+80685179 | MS.gene07781:CDS |
| TGATGCACACAAGTTGGTTC+TGG | 0.417382 | 8.1:-80684110 | None:intergenic |
| AGTGACATTCTAAGTATTGC+AGG | 0.426084 | 8.1:+80685806 | MS.gene07781:CDS |
| AATATGCTGTTCAATCTGAT+TGG | 0.427517 | 8.1:+80684231 | MS.gene07781:CDS |
| ACCAATGACGGTGGCGGCGG+CGG | 0.436786 | 8.1:+80685722 | MS.gene07781:CDS |
| ATTCTAAGTATTGCAGGGAT+TGG | 0.439295 | 8.1:+80685812 | MS.gene07781:CDS |
| ACATGAACAAGACTAGGGTT+AGG | 0.441835 | 8.1:-80685293 | None:intergenic |
| ACCCAATTTGATGAAATGTT+TGG | 0.443043 | 8.1:+80685635 | MS.gene07781:CDS |
| ATTTGTGAGATCTGCAACAA+AGG | 0.447156 | 8.1:+80683981 | MS.gene07781:CDS |
| GAAAACAAACCTGGGTTACC+AGG | 0.450492 | 8.1:-80683817 | None:intergenic |
| TCAAACTTAACTTCTGCATC+TGG | 0.461869 | 8.1:+80683676 | MS.gene07781:CDS |
| GATTCTCTCAATAATGTCTC+TGG | 0.462323 | 8.1:+80685467 | MS.gene07781:CDS |
| AAAGCCATCATGGCAAGGTT+AGG | 0.473421 | 8.1:+80685874 | MS.gene07781:CDS |
| TGGGTCTTAATATTCATAGT+TGG | 0.475879 | 8.1:-80685437 | None:intergenic |
| GGTGCAACAATGAATAGAAC+TGG | 0.479773 | 8.1:+80685401 | MS.gene07781:CDS |
| TCAAGTTCAACAACAATACT+TGG | 0.481347 | 8.1:+80683756 | MS.gene07781:CDS |
| CCAGGAACATGAACAAGACT+AGG | 0.482912 | 8.1:-80685299 | None:intergenic |
| GTTCTTTGCTTCAACTTCCA+TGG | 0.484808 | 8.1:-80684053 | None:intergenic |
| ATCAATCTCAAAAGCCATCA+TGG | 0.486220 | 8.1:+80685864 | MS.gene07781:CDS |
| CATGAACAAGACTAGGGTTA+GGG | 0.500721 | 8.1:-80685292 | None:intergenic |
| GGTTTGACAAGAGATTTCTT+GGG | 0.503476 | 8.1:+80685764 | MS.gene07781:CDS |
| ACCACCAATGACGGTGGCGG+CGG | 0.504903 | 8.1:+80685719 | MS.gene07781:CDS |
| TGGACCTAACCTTGCCATGA+TGG | 0.507628 | 8.1:-80685878 | None:intergenic |
| AGTATTGTTGTTGAACTTGA+GGG | 0.512238 | 8.1:-80683753 | None:intergenic |
| CATTATTGAGAGAATCAACT+TGG | 0.518652 | 8.1:-80685460 | None:intergenic |
| GAAGAAGTAGTTGCAGTAGT+TGG | 0.520425 | 8.1:-80685551 | None:intergenic |
| GGCGGCGGTGGTGGAAATGA+AGG | 0.520549 | 8.1:+80685743 | MS.gene07781:CDS |
| ATTTAGTTACCTTGAGAAGA+GGG | 0.520888 | 8.1:-80684306 | None:intergenic |
| GAGAGATGATCAACATTATG+TGG | 0.524492 | 8.1:-80685203 | None:intergenic |
| CAATTTGATGAAATGTTTGG+TGG | 0.530509 | 8.1:+80685638 | MS.gene07781:CDS |
| GAAAGCATGGTGAGAAGAAG+TGG | 0.533075 | 8.1:+80684186 | MS.gene07781:CDS |
| GCCGCCGCCACCGTCATTGG+TGG | 0.534618 | 8.1:-80685720 | None:intergenic |
| TGTGTGCTGAAGGTGCTGTT+TGG | 0.537428 | 8.1:-80685340 | None:intergenic |
| AAGTATTGTTGTTGAACTTG+AGG | 0.537745 | 8.1:-80683754 | None:intergenic |
| TTGCAGAAAGCAGCTCAAAT+GGG | 0.549522 | 8.1:+80685380 | MS.gene07781:CDS |
| GAAGGTGCTGTTTGGTAAGA+AGG | 0.550233 | 8.1:-80685332 | None:intergenic |
| AGAGCATTAGGGGATTTGAC+TGG | 0.562614 | 8.1:+80684143 | MS.gene07781:CDS |
| ACATTATGTGGGCCAAGCCA+AGG | 0.564626 | 8.1:-80685191 | None:intergenic |
| ATTGAGAGAATCAACTTGGT+GGG | 0.565155 | 8.1:-80685456 | None:intergenic |
| ACCTTTCTGAAATTCCACCT+TGG | 0.568922 | 8.1:+80685174 | MS.gene07781:CDS |
| GCCAAGGTGGAATTTCAGAA+AGG | 0.572630 | 8.1:-80685175 | None:intergenic |
| GTTCCAACAGCAACAAGTAG+TGG | 0.573848 | 8.1:+80683642 | MS.gene07781:CDS |
| GATGAAGGTTGTTGTTGTGA+GGG | 0.574323 | 8.1:-80685041 | None:intergenic |
| AATCTTCAACTTCACAGAAG+AGG | 0.577210 | 8.1:+80684020 | MS.gene07781:CDS |
| AATTTAGTTACCTTGAGAAG+AGG | 0.584975 | 8.1:-80684307 | None:intergenic |
| TAAGAAGGAAGTGTGGGACC+AGG | 0.585129 | 8.1:-80685317 | None:intergenic |
| TATTGAGAGAATCAACTTGG+TGG | 0.585481 | 8.1:-80685457 | None:intergenic |
| ACAACCACCAATGACGGTGG+CGG | 0.585647 | 8.1:+80685716 | MS.gene07781:CDS |
| AGAAACCTCCCTGGTAACCC+AGG | 0.588025 | 8.1:+80683808 | MS.gene07781:CDS |
| TCTAAAACAACCACCAATGA+CGG | 0.588296 | 8.1:+80685710 | MS.gene07781:CDS |
| AAATTATGAAGATCTTGTTG+AGG | 0.598408 | 8.1:-80685113 | None:intergenic |
| GTGACATTCTAAGTATTGCA+GGG | 0.598839 | 8.1:+80685807 | MS.gene07781:CDS |
| TGATGAAGGTTGTTGTTGTG+AGG | 0.603801 | 8.1:-80685042 | None:intergenic |
| GTTTGACAAGAGATTTCTTG+GGG | 0.605661 | 8.1:+80685765 | MS.gene07781:CDS |
| GAAGTAGTTGCAGTAGTTGG+TGG | 0.611520 | 8.1:-80685548 | None:intergenic |
| TGATGAGAAGATGATGATGA+AGG | 0.612181 | 8.1:-80685056 | None:intergenic |
| GTAGCTGACATGTGTGCTGA+AGG | 0.614431 | 8.1:-80685350 | None:intergenic |
| TGATCCATCAAGAGCATTAG+GGG | 0.622634 | 8.1:+80684133 | MS.gene07781:CDS |
| TCTCAAAAGCCATCATGGCA+AGG | 0.624497 | 8.1:+80685869 | MS.gene07781:CDS |
| TGGGTTGCTTCAAATCCAGA+AGG | 0.625506 | 8.1:-80685617 | None:intergenic |
| CAGGAACATGAACAAGACTA+GGG | 0.634110 | 8.1:-80685298 | None:intergenic |
| ACTGTGGAACCCTCTTCTCA+AGG | 0.634375 | 8.1:+80684297 | MS.gene07781:CDS |
| AGAATGTCACTATGTGACAA+TGG | 0.635720 | 8.1:-80685794 | None:intergenic |
| GAAGAGGTCATAATTTACCA+TGG | 0.635939 | 8.1:+80684036 | MS.gene07781:CDS |
| TAGCTATAACCTCAGCATCA+GGG | 0.639613 | 8.1:-80683923 | None:intergenic |
| AGAGATGATCAACATTATGT+GGG | 0.641603 | 8.1:-80685202 | None:intergenic |
| GGATCATGATGCACACAAGT+TGG | 0.642093 | 8.1:-80684116 | None:intergenic |
| AAAACAAACCTGGGTTACCA+GGG | 0.660704 | 8.1:-80683816 | None:intergenic |
| ACAAACCTGGGTTACCAGGG+AGG | 0.662065 | 8.1:-80683813 | None:intergenic |
| AAAACAACCACCAATGACGG+TGG | 0.670222 | 8.1:+80685713 | MS.gene07781:CDS |
| GTTTGGTAAGAAGGAAGTGT+GGG | 0.674496 | 8.1:-80685323 | None:intergenic |
| AAAGCTCATTCCAAAACATG+TGG | 0.678165 | 8.1:+80684254 | MS.gene07781:CDS |
| TGTTTGGTAAGAAGGAAGTG+TGG | 0.686026 | 8.1:-80685324 | None:intergenic |
| TTATGTGGGCCAAGCCAAGG+TGG | 0.692215 | 8.1:-80685188 | None:intergenic |
| AGAGAATATAGATGTGACTG+TGG | 0.703138 | 8.1:+80684281 | MS.gene07781:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TATTAATATTATACATCTTC+CGG | + | chr8.1:80684453-80684472 | MS.gene07781:intron | 15.0% |
| !! | TTAATTTCATTCATTTATGA+AGG | + | chr8.1:80684375-80684394 | MS.gene07781:intron | 15.0% |
| !! | TTGATAAACATGTTATTAAT+GGG | + | chr8.1:80683865-80683884 | MS.gene07781:intron | 15.0% |
| !! | TTTGATAAACATGTTATTAA+TGG | + | chr8.1:80683864-80683883 | MS.gene07781:intron | 15.0% |
| !! | AACAAAACAAAACAAAAAGA+AGG | - | chr8.1:80683845-80683864 | None:intergenic | 20.0% |
| !! | AAGACAAATAGTCTATTATT+AGG | - | chr8.1:80684766-80684785 | None:intergenic | 20.0% |
| !! | CATTAAAAAATTTCCTTACT+AGG | - | chr8.1:80684738-80684757 | None:intergenic | 20.0% |
| !!! | TTAATGGGTTTTTTTTTGTT+TGG | + | chr8.1:80683880-80683899 | MS.gene07781:intron | 20.0% |
| ! | AAAAAGTAAAGAGAGAGAAA+AGG | + | chr8.1:80684819-80684838 | MS.gene07781:intron | 25.0% |
| ! | AAATTATGAAGATCTTGTTG+AGG | - | chr8.1:80685116-80685135 | None:intergenic | 25.0% |
| ! | ACCAAACATTTCATCAAATT+GGG | - | chr8.1:80685639-80685658 | None:intergenic | 25.0% |
| ! | AGAATGGAATTAGTGATTAT+GGG | - | chr8.1:80684697-80684716 | None:intergenic | 25.0% |
| ! | ATTTCATTCATTTATGAAGG+TGG | + | chr8.1:80684378-80684397 | MS.gene07781:intron | 25.0% |
| !!! | AGGTTTCTCTTTTTTTTCAT+TGG | - | chr8.1:80683796-80683815 | None:intergenic | 25.0% |
| !!! | GTTGCTCTTTTTTCAATAAT+GGG | - | chr8.1:80685139-80685158 | None:intergenic | 25.0% |
| !!! | TGTTGCTCTTTTTTCAATAA+TGG | - | chr8.1:80685140-80685159 | None:intergenic | 25.0% |
| !!! | TTTTTTGCTAGTAAACTCTT+TGG | - | chr8.1:80683954-80683973 | None:intergenic | 25.0% |
| !!! | TTTTTTTTCATTGGTTGAGT+TGG | - | chr8.1:80683787-80683806 | None:intergenic | 25.0% |
| AAAAGTGGAAATTCCTAGTA+AGG | + | chr8.1:80684722-80684741 | MS.gene07781:intron | 30.0% | |
| AATAATGTCTCTGGTAACTT+TGG | + | chr8.1:80685476-80685495 | MS.gene07781:CDS | 30.0% | |
| AATATGCTGTTCAATCTGAT+TGG | + | chr8.1:80684231-80684250 | MS.gene07781:CDS | 30.0% | |
| AATTTAGTTACCTTGAGAAG+AGG | - | chr8.1:80684310-80684329 | None:intergenic | 30.0% | |
| ACACACTTGATTTCTTACAT+TGG | + | chr8.1:80684637-80684656 | MS.gene07781:intron | 30.0% | |
| ACCCAATTTGATGAAATGTT+TGG | + | chr8.1:80685635-80685654 | MS.gene07781:CDS | 30.0% | |
| AGAGATGATCAACATTATGT+GGG | - | chr8.1:80685205-80685224 | None:intergenic | 30.0% | |
| AGAGTTTACTAGCAAAAAAC+AGG | + | chr8.1:80683955-80683974 | MS.gene07781:CDS | 30.0% | |
| ATTTAGTTACCTTGAGAAGA+GGG | - | chr8.1:80684309-80684328 | None:intergenic | 30.0% | |
| CAATTTGATGAAATGTTTGG+TGG | + | chr8.1:80685638-80685657 | MS.gene07781:CDS | 30.0% | |
| CACCAAACATTTCATCAAAT+TGG | - | chr8.1:80685640-80685659 | None:intergenic | 30.0% | |
| CAGAATGGAATTAGTGATTA+TGG | - | chr8.1:80684698-80684717 | None:intergenic | 30.0% | |
| CATTATTGAGAGAATCAACT+TGG | - | chr8.1:80685463-80685482 | None:intergenic | 30.0% | |
| CTTGTTGAGGATTATTGAAT+TGG | - | chr8.1:80685103-80685122 | None:intergenic | 30.0% | |
| TAATCACTAATTCCATTCTG+TGG | + | chr8.1:80684698-80684717 | MS.gene07781:intron | 30.0% | |
| TCAAGTTCAACAACAATACT+TGG | + | chr8.1:80683756-80683775 | MS.gene07781:CDS | 30.0% | |
| TGCTTATAATAGTGATCAGA+GGG | + | chr8.1:80684589-80684608 | MS.gene07781:intron | 30.0% | |
| TGGGTCTTAATATTCATAGT+TGG | - | chr8.1:80685440-80685459 | None:intergenic | 30.0% | |
| TTGCTTATAATAGTGATCAG+AGG | + | chr8.1:80684588-80684607 | MS.gene07781:intron | 30.0% | |
| ! | AAGTATTGTTGTTGAACTTG+AGG | - | chr8.1:80683757-80683776 | None:intergenic | 30.0% |
| ! | AGTATTGTTGTTGAACTTGA+GGG | - | chr8.1:80683756-80683775 | None:intergenic | 30.0% |
| ! | TTATGATGAGTTTTGCAGAA+GGG | + | chr8.1:80684932-80684951 | MS.gene07781:intron | 30.0% |
| ! | TTTATGATGAGTTTTGCAGA+AGG | + | chr8.1:80684931-80684950 | MS.gene07781:intron | 30.0% |
| ! | TTTTGCTTATAACAGTGAAC+CGG | - | chr8.1:80684475-80684494 | None:intergenic | 30.0% |
| !!! | TAGGTTTTGAATTGTATCCT+AGG | + | chr8.1:80684404-80684423 | MS.gene07781:intron | 30.0% |
| AAAAAAAAGAGAAACCTCCC+TGG | + | chr8.1:80683799-80683818 | MS.gene07781:CDS | 35.0% | |
| AAAGCTCATTCCAAAACATG+TGG | + | chr8.1:80684254-80684273 | MS.gene07781:CDS | 35.0% | |
| AAGAAGGTTGAAAACAAACC+TGG | - | chr8.1:80683829-80683848 | None:intergenic | 35.0% | |
| AAGTAAAGAGAGAGAAAAGG+AGG | + | chr8.1:80684822-80684841 | MS.gene07781:intron | 35.0% | |
| AATCTTCAACTTCACAGAAG+AGG | + | chr8.1:80684020-80684039 | MS.gene07781:CDS | 35.0% | |
| AGAAGGTTGAAAACAAACCT+GGG | - | chr8.1:80683828-80683847 | None:intergenic | 35.0% | |
| AGAATGTCACTATGTGACAA+TGG | - | chr8.1:80685797-80685816 | None:intergenic | 35.0% | |
| AGAGAATATAGATGTGACTG+TGG | + | chr8.1:80684281-80684300 | MS.gene07781:CDS | 35.0% | |
| AGGTTTGACAAGAGATTTCT+TGG | + | chr8.1:80685763-80685782 | MS.gene07781:CDS | 35.0% | |
| AGTAAAGAGAGAGAAAAGGA+GGG | + | chr8.1:80684823-80684842 | MS.gene07781:intron | 35.0% | |
| ATCAATCTCAAAAGCCATCA+TGG | + | chr8.1:80685864-80685883 | MS.gene07781:CDS | 35.0% | |
| ATTCCATTCTGTGGAAAAAG+TGG | + | chr8.1:80684707-80684726 | MS.gene07781:intron | 35.0% | |
| ATTGAGAGAATCAACTTGGT+GGG | - | chr8.1:80685459-80685478 | None:intergenic | 35.0% | |
| ATTTGTGAGATCTGCAACAA+AGG | + | chr8.1:80683981-80684000 | MS.gene07781:CDS | 35.0% | |
| GAAGAGGTCATAATTTACCA+TGG | + | chr8.1:80684036-80684055 | MS.gene07781:CDS | 35.0% | |
| GAGAGATGATCAACATTATG+TGG | - | chr8.1:80685206-80685225 | None:intergenic | 35.0% | |
| GATTCTCTCAATAATGTCTC+TGG | + | chr8.1:80685467-80685486 | MS.gene07781:CDS | 35.0% | |
| GGTTTGACAAGAGATTTCTT+GGG | + | chr8.1:80685764-80685783 | MS.gene07781:CDS | 35.0% | |
| GTTTGACAAGAGATTTCTTG+GGG | + | chr8.1:80685765-80685784 | MS.gene07781:CDS | 35.0% | |
| TATTGAGAGAATCAACTTGG+TGG | - | chr8.1:80685460-80685479 | None:intergenic | 35.0% | |
| TCAAACTTAACTTCTGCATC+TGG | + | chr8.1:80683676-80683695 | MS.gene07781:CDS | 35.0% | |
| TCATTTATGAAGGTGGTCAT+AGG | + | chr8.1:80684385-80684404 | MS.gene07781:intron | 35.0% | |
| TCTGGTAACAGAACTGAAAT+TGG | + | chr8.1:80683715-80683734 | MS.gene07781:CDS | 35.0% | |
| TGTTGTTGTGAGGGTAAAAT+TGG | - | chr8.1:80685035-80685054 | None:intergenic | 35.0% | |
| ! | AGTGACATTCTAAGTATTGC+AGG | + | chr8.1:80685806-80685825 | MS.gene07781:CDS | 35.0% |
| ! | ATTCTAAGTATTGCAGGGAT+TGG | + | chr8.1:80685812-80685831 | MS.gene07781:CDS | 35.0% |
| ! | ATTGTATCCTAGGTTGACTT+TGG | + | chr8.1:80684414-80684433 | MS.gene07781:intron | 35.0% |
| ! | GTGACATTCTAAGTATTGCA+GGG | + | chr8.1:80685807-80685826 | MS.gene07781:CDS | 35.0% |
| ! | TCTAAAACAACCACCAATGA+CGG | + | chr8.1:80685710-80685729 | MS.gene07781:CDS | 35.0% |
| ! | TGATGAGAAGATGATGATGA+AGG | - | chr8.1:80685059-80685078 | None:intergenic | 35.0% |
| ! | TTTTCTTCTTCATCTCCTTC+TGG | + | chr8.1:80685602-80685621 | MS.gene07781:CDS | 35.0% |
| !! | ATGATCCATCAAGAGCATTA+GGG | + | chr8.1:80684132-80684151 | MS.gene07781:CDS | 35.0% |
| !! | TAGAGCATTTTGTGATGCAT+TGG | + | chr8.1:80684971-80684990 | MS.gene07781:CDS | 35.0% |
| !!! | TTTCCACTTTTTCCACAGAA+TGG | - | chr8.1:80684713-80684732 | None:intergenic | 35.0% |
| !!! | TTTTTCATTGGTTGAGTTGG+AGG | - | chr8.1:80683784-80683803 | None:intergenic | 35.0% |
| AAAACAAACCTGGGTTACCA+GGG | - | chr8.1:80683819-80683838 | None:intergenic | 40.0% | |
| AAATCCCCTAATGCTCTTGA+TGG | - | chr8.1:80684140-80684159 | None:intergenic | 40.0% | |
| ACATGAACAAGACTAGGGTT+AGG | - | chr8.1:80685296-80685315 | None:intergenic | 40.0% | |
| ATAGCTATAACCTCAGCATC+AGG | - | chr8.1:80683927-80683946 | None:intergenic | 40.0% | |
| CAGGAACATGAACAAGACTA+GGG | - | chr8.1:80685301-80685320 | None:intergenic | 40.0% | |
| CATGAACAAGACTAGGGTTA+GGG | - | chr8.1:80685295-80685314 | None:intergenic | 40.0% | |
| GAAGAAGTAGTTGCAGTAGT+TGG | - | chr8.1:80685554-80685573 | None:intergenic | 40.0% | |
| GGTGCAACAATGAATAGAAC+TGG | + | chr8.1:80685401-80685420 | MS.gene07781:CDS | 40.0% | |
| GTTCTTTGCTTCAACTTCCA+TGG | - | chr8.1:80684056-80684075 | None:intergenic | 40.0% | |
| TAGCTATAACCTCAGCATCA+GGG | - | chr8.1:80683926-80683945 | None:intergenic | 40.0% | |
| TGTTTGGTAAGAAGGAAGTG+TGG | - | chr8.1:80685327-80685346 | None:intergenic | 40.0% | |
| TTATGCACCAAAGTCAACCT+AGG | - | chr8.1:80684424-80684443 | None:intergenic | 40.0% | |
| TTGCAGAAAGCAGCTCAAAT+GGG | + | chr8.1:80685380-80685399 | MS.gene07781:CDS | 40.0% | |
| ! | AAGCACTTTTCCAGAAAGCA+TGG | + | chr8.1:80684173-80684192 | MS.gene07781:CDS | 40.0% |
| ! | GATGAAGGTTGTTGTTGTGA+GGG | - | chr8.1:80685044-80685063 | None:intergenic | 40.0% |
| ! | GTTTGGTAAGAAGGAAGTGT+GGG | - | chr8.1:80685326-80685345 | None:intergenic | 40.0% |
| ! | TGATGAAGGTTGTTGTTGTG+AGG | - | chr8.1:80685045-80685064 | None:intergenic | 40.0% |
| ! | TTCTCTAGTGCCACATGTTT+TGG | - | chr8.1:80684267-80684286 | None:intergenic | 40.0% |
| !! | ACCTTTCTGAAATTCCACCT+TGG | + | chr8.1:80685174-80685193 | MS.gene07781:CDS | 40.0% |
| !! | CATGATCCATCAAGAGCATT+AGG | + | chr8.1:80684131-80684150 | MS.gene07781:CDS | 40.0% |
| !! | TGATCCATCAAGAGCATTAG+GGG | + | chr8.1:80684133-80684152 | MS.gene07781:CDS | 40.0% |
| AAAACAACCACCAATGACGG+TGG | + | chr8.1:80685713-80685732 | MS.gene07781:CDS | 45.0% | |
| CAAGAGATTTCTTGGGGCTT+AGG | + | chr8.1:80685771-80685790 | MS.gene07781:CDS | 45.0% | |
| CCAGGAACATGAACAAGACT+AGG | - | chr8.1:80685302-80685321 | None:intergenic | 45.0% | |
| CTTCTTCTCACCATGCTTTC+TGG | - | chr8.1:80684186-80684205 | None:intergenic | 45.0% | |
| GAAAACAAACCTGGGTTACC+AGG | - | chr8.1:80683820-80683839 | None:intergenic | 45.0% | |
| GAAGTAGTTGCAGTAGTTGG+TGG | - | chr8.1:80685551-80685570 | None:intergenic | 45.0% | |
| GCCAAGGTGGAATTTCAGAA+AGG | - | chr8.1:80685178-80685197 | None:intergenic | 45.0% | |
| GGATCATGATGCACACAAGT+TGG | - | chr8.1:80684119-80684138 | None:intergenic | 45.0% | |
| GGTGAAGCTAGTGTTTCTTC+TGG | + | chr8.1:80683697-80683716 | MS.gene07781:CDS | 45.0% | |
| GTTCCAACAGCAACAAGTAG+TGG | + | chr8.1:80683642-80683661 | MS.gene07781:CDS | 45.0% | |
| GTTGCAGAAAGCAGCTCAAA+TGG | + | chr8.1:80685379-80685398 | MS.gene07781:CDS | 45.0% | |
| TCTCAAAAGCCATCATGGCA+AGG | + | chr8.1:80685869-80685888 | MS.gene07781:CDS | 45.0% | |
| TGGGTTGCTTCAAATCCAGA+AGG | - | chr8.1:80685620-80685639 | None:intergenic | 45.0% | |
| ! | AGAGCATTAGGGGATTTGAC+TGG | + | chr8.1:80684143-80684162 | MS.gene07781:CDS | 45.0% |
| ! | CTTCCACTACTTGTTGCTGT+TGG | - | chr8.1:80683648-80683667 | None:intergenic | 45.0% |
| ! | GAAAGCATGGTGAGAAGAAG+TGG | + | chr8.1:80684186-80684205 | MS.gene07781:CDS | 45.0% |
| ! | TCTGAAATTCCACCTTGGCT+TGG | + | chr8.1:80685179-80685198 | MS.gene07781:CDS | 45.0% |
| !! | CCTAGTCTTGTTCATGTTCC+TGG | + | chr8.1:80685299-80685318 | MS.gene07781:CDS | 45.0% |
| !! | GAAGGTGCTGTTTGGTAAGA+AGG | - | chr8.1:80685335-80685354 | None:intergenic | 45.0% |
| !! | TGATGCACACAAGTTGGTTC+TGG | - | chr8.1:80684113-80684132 | None:intergenic | 45.0% |
| !! | TTTTTCAGACCCTGATGCTG+AGG | + | chr8.1:80683914-80683933 | MS.gene07781:intron | 45.0% |
| ACATTATGTGGGCCAAGCCA+AGG | - | chr8.1:80685194-80685213 | None:intergenic | 50.0% | |
| ACTGTGGAACCCTCTTCTCA+AGG | + | chr8.1:80684297-80684316 | MS.gene07781:CDS | 50.0% | |
| GTAGCTGACATGTGTGCTGA+AGG | - | chr8.1:80685353-80685372 | None:intergenic | 50.0% | |
| ! | TAAGAAGGAAGTGTGGGACC+AGG | - | chr8.1:80685320-80685339 | None:intergenic | 50.0% |
| !! | TGTGTGCTGAAGGTGCTGTT+TGG | - | chr8.1:80685343-80685362 | None:intergenic | 50.0% |
| ACAAACCTGGGTTACCAGGG+AGG | - | chr8.1:80683816-80683835 | None:intergenic | 55.0% | |
| ACAACCACCAATGACGGTGG+CGG | + | chr8.1:80685716-80685735 | MS.gene07781:CDS | 55.0% | |
| AGAAACCTCCCTGGTAACCC+AGG | + | chr8.1:80683808-80683827 | MS.gene07781:CDS | 55.0% | |
| TTATGTGGGCCAAGCCAAGG+TGG | - | chr8.1:80685191-80685210 | None:intergenic | 55.0% | |
| ! | ACCACCAATGACGGTGGCGG+CGG | + | chr8.1:80685719-80685738 | MS.gene07781:CDS | 65.0% |
| !! | GGCGGCGGTGGTGGAAATGA+AGG | + | chr8.1:80685743-80685762 | MS.gene07781:CDS | 65.0% |
| ! | ACCAATGACGGTGGCGGCGG+CGG | + | chr8.1:80685722-80685741 | MS.gene07781:CDS | 70.0% |
| GCCGCCGCCACCGTCATTGG+TGG | - | chr8.1:80685723-80685742 | None:intergenic | 75.0% | |
| ! | AATGACGGTGGCGGCGGCGG+CGG | + | chr8.1:80685725-80685744 | MS.gene07781:CDS | 75.0% |
| ! | GCCGCCGCCGCCACCGTCAT+TGG | - | chr8.1:80685726-80685745 | None:intergenic | 80.0% |
| !! | GACGGTGGCGGCGGCGGCGG+CGG | + | chr8.1:80685728-80685747 | MS.gene07781:CDS | 90.0% |
| ! | GGCGGCGGCGGCGGCGGTGG+TGG | + | chr8.1:80685734-80685753 | MS.gene07781:CDS | 95.0% |
| !! | GGTGGCGGCGGCGGCGGCGG+TGG | + | chr8.1:80685731-80685750 | MS.gene07781:CDS | 95.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 80683622 | 80685895 | 80683622 | ID=MS.gene07781 |
| chr8.1 | mRNA | 80683622 | 80685895 | 80683622 | ID=MS.gene07781.t1;Parent=MS.gene07781 |
| chr8.1 | exon | 80683622 | 80683829 | 80683622 | ID=MS.gene07781.t1.exon1;Parent=MS.gene07781.t1 |
| chr8.1 | CDS | 80683622 | 80683829 | 80683622 | ID=cds.MS.gene07781.t1;Parent=MS.gene07781.t1 |
| chr8.1 | exon | 80683922 | 80684318 | 80683922 | ID=MS.gene07781.t1.exon2;Parent=MS.gene07781.t1 |
| chr8.1 | CDS | 80683922 | 80684318 | 80683922 | ID=cds.MS.gene07781.t1;Parent=MS.gene07781.t1 |
| chr8.1 | exon | 80684950 | 80685895 | 80684950 | ID=MS.gene07781.t1.exon3;Parent=MS.gene07781.t1 |
| chr8.1 | CDS | 80684950 | 80685895 | 80684950 | ID=cds.MS.gene07781.t1;Parent=MS.gene07781.t1 |
| Gene Sequence |
| Protein sequence |
