AlfalfaGEDB | MS.gene052725

M. sativa cultivar XinJiangDaYe / MS.gene052725

KEGG: -
GO: GO:0003674 GO:0003676 GO:0005488 GO:0097159 GO:1901363
PFAM: PF12171.11

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene052725.t1	XP_003627109.1	99	204	0	1	1	202	1	204	2.50E-111	411.4

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene052725.t1	Q8H1F5	78.6	187	37	3	18	202	21	206	6.0e-82	305.1

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene052725.t1	G7LHG9	99.0	204	0	1	1	202	1	204	1.8e-111	411.4

TFs/TRs:

Gene ID	Type	Classification
MS.gene052725.t1	TF	C2H2

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene052723	MS.gene052725	0.911649	4.79E-83	-1.69E-46
MS.gene052725	MS.gene07781	0.802225	6.21E-49	-1.69E-46
MS.gene052725	MS.gene07783	0.945999	1.06E-104	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene052725.t1	MTR_8g017210	99.020	204	0	1	1	202	1	204	8.72e-151	428
MS.gene052725.t1	MTR_2g099990	82.292	192	23	4	18	202	1	188	1.30e-109	322
MS.gene052725.t1	MTR_1g094115	88.276	145	17	0	58	202	45	189	2.99e-98	295
MS.gene052725.t1	MTR_4g059870	79.429	175	34	2	29	202	8	181	3.01e-98	294
MS.gene052725.t1	MTR_1g016010	71.921	203	37	3	1	202	1	184	1.87e-96	288
MS.gene052725.t1	MTR_1g112270	87.857	140	17	0	63	202	47	186	4.81e-95	288
MS.gene052725.t1	MTR_2g093960	86.207	145	19	1	59	202	31	175	4.27e-93	278
MS.gene052725.t1	MTR_1g112270	81.457	151	17	1	63	202	47	197	2.40e-91	278
MS.gene052725.t1	MTR_2g090745	86.466	133	18	0	70	202	17	149	1.73e-85	259
MS.gene052725.t1	MTR_2g099990	94.958	119	6	0	84	202	1	119	5.61e-84	254
MS.gene052725.t1	MTR_1g094115	78.621	145	14	1	58	202	45	172	4.54e-82	253
MS.gene052725.t1	MTR_2g017720	76.667	120	23	1	65	184	318	432	4.86e-59	191
MS.gene052725.t1	MTR_3g102000	71.717	99	21	2	87	181	36	131	5.31e-43	144
MS.gene052725.t1	MTR_5g030560	72.000	50	14	0	65	114	11	60	1.46e-20	82.0
MS.gene052725.t1	MTR_1g093095	35.385	130	70	5	83	200	109	236	2.16e-18	81.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene052725.t1	AT1G55110	78.534	191	38	3	14	202	17	206	1.74e-107	316
MS.gene052725.t1	AT1G55110	78.534	191	38	3	14	202	17	206	1.74e-107	316
MS.gene052725.t1	AT1G55110	78.534	191	38	3	14	202	17	206	1.74e-107	316
MS.gene052725.t1	AT3G13810	78.173	197	34	3	15	202	17	213	5.28e-106	314
MS.gene052725.t1	AT3G13810	78.173	197	34	3	15	202	17	213	5.28e-106	314
MS.gene052725.t1	AT3G13810	78.173	197	34	3	15	202	12	208	6.13e-106	314
MS.gene052725.t1	AT3G13810	78.351	194	33	3	18	202	1	194	3.57e-104	309
MS.gene052725.t1	AT5G66730	73.262	187	33	2	17	202	4	174	6.44e-98	293
MS.gene052725.t1	AT3G50700	70.899	189	36	2	17	202	4	176	1.38e-97	291
MS.gene052725.t1	AT1G03840	85.430	151	20	1	52	202	35	183	1.39e-96	290
MS.gene052725.t1	AT1G14580	70.879	182	49	1	21	202	18	195	1.70e-96	288
MS.gene052725.t1	AT1G14580	70.879	182	49	1	21	202	18	195	1.70e-96	288
MS.gene052725.t1	AT1G14580	70.879	182	49	1	21	202	32	209	2.70e-96	288
MS.gene052725.t1	AT2G02080	83.448	145	24	0	58	202	52	196	1.27e-95	288
MS.gene052725.t1	AT2G02080	83.448	145	24	0	58	202	52	196	1.27e-95	288
MS.gene052725.t1	AT2G02080	83.448	145	24	0	58	202	52	196	1.27e-95	288
MS.gene052725.t1	AT2G02080	83.448	145	24	0	58	202	52	196	1.27e-95	288
MS.gene052725.t1	AT5G44160	75.862	174	40	1	29	202	8	179	2.62e-95	285
MS.gene052725.t1	AT3G45260	86.806	144	18	1	60	202	39	182	2.73e-94	282
MS.gene052725.t1	AT3G45260	86.806	144	18	1	60	202	39	182	2.73e-94	282
MS.gene052725.t1	AT4G02670	75.556	180	40	3	27	202	19	198	7.34e-94	280
MS.gene052725.t1	AT4G02670	75.556	180	40	3	27	202	17	196	8.40e-94	279
MS.gene052725.t1	AT1G03840	84.106	151	20	2	52	202	35	181	9.55e-93	280
MS.gene052725.t1	AT3G13810	71.921	203	42	4	15	202	12	214	1.22e-91	277
MS.gene052725.t1	AT5G03150	85.417	144	20	1	60	202	53	196	1.24e-91	277
MS.gene052725.t1	AT5G60470	76.220	164	35	2	40	202	23	183	1.29e-91	275
MS.gene052725.t1	AT5G60470	76.220	164	35	2	40	202	27	187	1.34e-91	276
MS.gene052725.t1	AT2G02070	83.217	143	24	0	60	202	52	194	1.20e-90	277
MS.gene052725.t1	AT2G02070	83.217	143	24	0	60	202	52	194	1.20e-90	277
MS.gene052725.t1	AT3G13810	72.000	200	41	4	18	202	1	200	6.54e-90	273
MS.gene052725.t1	AT5G44160	85.714	133	19	0	70	202	8	140	1.12e-85	259
MS.gene052725.t1	AT5G60470	74.510	153	37	2	52	202	26	178	1.14e-82	253
MS.gene052725.t1	AT2G02080	85.714	119	17	0	84	202	1	119	8.40e-80	245
MS.gene052725.t1	AT2G02080	85.714	119	17	0	84	202	1	119	8.40e-80	245
MS.gene052725.t1	AT1G25250	67.123	146	45	2	60	202	33	178	8.35e-72	223
MS.gene052725.t1	AT1G68130	66.897	145	46	2	60	202	41	185	2.22e-71	223
MS.gene052725.t1	AT2G01940	65.772	149	49	2	56	202	40	188	4.03e-71	223
MS.gene052725.t1	AT1G25250	63.816	152	50	3	56	202	4	155	1.85e-69	216
MS.gene052725.t1	AT2G01940	64.667	150	50	3	56	203	40	188	5.83e-69	217
MS.gene052725.t1	AT1G68130	68.687	99	29	2	106	202	1	99	8.03e-45	152
MS.gene052725.t1	AT1G25250	68.000	100	29	2	106	202	1	100	1.50e-44	150
MS.gene052725.t1	AT2G01940	66.667	99	31	2	106	202	1	99	2.35e-43	149
MS.gene052725.t1	AT1G34370	33.784	148	84	3	69	202	14	161	2.33e-22	92.0
MS.gene052725.t1	AT1G34370	33.784	148	84	3	69	202	224	371	7.55e-22	92.8
MS.gene052725.t1	AT1G34370	33.784	148	84	3	69	202	224	371	7.55e-22	92.8
MS.gene052725.t1	AT5G22890	34.615	156	86	5	61	202	191	344	1.86e-21	90.9
MS.gene052725.t1	AT1G08290	28.037	214	131	7	9	200	87	299	1.32e-18	82.8
MS.gene052725.t1	AT1G13290	35.938	128	71	5	83	200	95	221	9.85e-18	79.7
MS.gene052725.t1	AT1G51220	33.333	126	75	3	83	200	172	296	1.36e-17	79.7
MS.gene052725.t1	AT3G20880	33.083	133	80	3	76	200	243	374	1.85e-17	80.1
MS.gene052725.t1	AT3G57670	33.858	127	73	4	83	200	211	335	3.43e-17	79.0
MS.gene052725.t1	AT1G34790	33.824	136	81	3	73	200	129	263	1.79e-16	76.3

Find 36 sgRNAs with CRISPR-Local

Find 50 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTTCTTCTCACCATGCTTTC+TGG	0.052883	8.1:-80657697	None:intergenic
TTCTCTAGTGCCACATGTTT+TGG	0.232714	8.1:-80657778	None:intergenic
TGTAATCATAAGATGGTAAA+AGG	0.239938	8.1:+80657124	None:intergenic
TCTGGTAACAGAACTGAAAT+TGG	0.252456	8.1:+80657229	MS.gene052725:CDS
CATGATCCATCAAGAGCATT+AGG	0.324615	8.1:+80657645	MS.gene052725:CDS
ATGATCCATCAAGAGCATTA+GGG	0.372273	8.1:+80657646	MS.gene052725:CDS
TCATTTATGAAGGTGGTCAT+AGG	0.384432	8.1:+80657899	MS.gene052725:intron
ATAGCTATAACCTCAGCATC+AGG	0.391905	8.1:-80657438	None:intergenic
CTTCCACTACTTGTTGCTGT+TGG	0.402652	8.1:-80657159	None:intergenic
GGTGAAGCTAGTGTTTCTTC+TGG	0.407702	8.1:+80657211	MS.gene052725:CDS
AAATCCCCTAATGCTCTTGA+TGG	0.408372	8.1:-80657651	None:intergenic
TGATGCACACAAGTTGGTTC+TGG	0.417382	8.1:-80657624	None:intergenic
AATATGCTGTTCAATCTGAT+TGG	0.427517	8.1:+80657745	MS.gene052725:CDS
ATTTGTGAGATCTGCAACAA+AGG	0.447156	8.1:+80657495	MS.gene052725:CDS
GAAAACAAACCTGGGTTACC+AGG	0.450492	8.1:-80657331	None:intergenic
TCAAACTTAACTTCTGCATC+TGG	0.461869	8.1:+80657190	MS.gene052725:CDS
TCAAGTTCAACAACAATACT+TGG	0.481347	8.1:+80657270	MS.gene052725:CDS
GTTCTTTGCTTCAACTTCCA+TGG	0.484808	8.1:-80657567	None:intergenic
AGTATTGTTGTTGAACTTGA+GGG	0.512238	8.1:-80657267	None:intergenic
ATTTAGTTACCTTGAGAAGA+GGG	0.520888	8.1:-80657820	None:intergenic
GAAAGCATGGTGAGAAGAAG+TGG	0.533075	8.1:+80657700	MS.gene052725:CDS
AAGTATTGTTGTTGAACTTG+AGG	0.537745	8.1:-80657268	None:intergenic
AGAGCATTAGGGGATTTGAC+TGG	0.562614	8.1:+80657657	MS.gene052725:CDS
GTTCCAACAGCAACAAGTAG+TGG	0.573848	8.1:+80657156	MS.gene052725:CDS
AATCTTCAACTTCACAGAAG+AGG	0.577210	8.1:+80657534	MS.gene052725:CDS
AATTTAGTTACCTTGAGAAG+AGG	0.584975	8.1:-80657821	None:intergenic
AGAAACCTCCCTGGTAACCC+AGG	0.588025	8.1:+80657322	MS.gene052725:CDS
TGATCCATCAAGAGCATTAG+GGG	0.622634	8.1:+80657647	MS.gene052725:CDS
ACTGTGGAACCCTCTTCTCA+AGG	0.634375	8.1:+80657811	MS.gene052725:CDS
GAAGAGGTCATAATTTACCA+TGG	0.635939	8.1:+80657550	MS.gene052725:CDS
TAGCTATAACCTCAGCATCA+GGG	0.639613	8.1:-80657437	None:intergenic
GGATCATGATGCACACAAGT+TGG	0.642093	8.1:-80657630	None:intergenic
AAAACAAACCTGGGTTACCA+GGG	0.660704	8.1:-80657330	None:intergenic
ACAAACCTGGGTTACCAGGG+AGG	0.662065	8.1:-80657327	None:intergenic
AAAGCTCATTCCAAAACATG+TGG	0.678165	8.1:+80657768	MS.gene052725:CDS
AGAGAATATAGATGTGACTG+TGG	0.703138	8.1:+80657795	MS.gene052725:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TTAATTTCATTCATTTATGA+AGG	+	chr8.1:80657889-80657908	MS.gene052725:intron	15.0%
!!	TTGATAAACATGTTATTAAT+GGG	+	chr8.1:80657379-80657398	MS.gene052725:intron	15.0%
!!	TTTGATAAACATGTTATTAA+TGG	+	chr8.1:80657378-80657397	MS.gene052725:intron	15.0%
!!	AACAAAACAAAACAAAAAGA+AGG	-	chr8.1:80657359-80657378	None:intergenic	20.0%
!!!	TTAATGGGTTTTTTTTTGTT+TGG	+	chr8.1:80657394-80657413	MS.gene052725:intron	20.0%
!	ATTTCATTCATTTATGAAGG+TGG	+	chr8.1:80657892-80657911	MS.gene052725:intron	25.0%
!!!	AGGTTTCTCTTTTTTTTCAT+TGG	-	chr8.1:80657310-80657329	None:intergenic	25.0%
!!!	TTTTTTGCTAGTAAACTCTT+TGG	-	chr8.1:80657468-80657487	None:intergenic	25.0%
!!!	TTTTTTTTCATTGGTTGAGT+TGG	-	chr8.1:80657301-80657320	None:intergenic	25.0%
	AATATGCTGTTCAATCTGAT+TGG	+	chr8.1:80657745-80657764	MS.gene052725:CDS	30.0%
	AATTTAGTTACCTTGAGAAG+AGG	-	chr8.1:80657824-80657843	None:intergenic	30.0%
	AGAGTTTACTAGCAAAAAAC+AGG	+	chr8.1:80657469-80657488	MS.gene052725:CDS	30.0%
	ATTTAGTTACCTTGAGAAGA+GGG	-	chr8.1:80657823-80657842	None:intergenic	30.0%
	TCAAGTTCAACAACAATACT+TGG	+	chr8.1:80657270-80657289	MS.gene052725:CDS	30.0%
!	AAGTATTGTTGTTGAACTTG+AGG	-	chr8.1:80657271-80657290	None:intergenic	30.0%
!	AGTATTGTTGTTGAACTTGA+GGG	-	chr8.1:80657270-80657289	None:intergenic	30.0%
	AAAAAAAAGAGAAACCTCCC+TGG	+	chr8.1:80657313-80657332	MS.gene052725:CDS	35.0%
	AAAGCTCATTCCAAAACATG+TGG	+	chr8.1:80657768-80657787	MS.gene052725:CDS	35.0%
	AAGAAGGTTGAAAACAAACC+TGG	-	chr8.1:80657343-80657362	None:intergenic	35.0%
	AATCTTCAACTTCACAGAAG+AGG	+	chr8.1:80657534-80657553	MS.gene052725:CDS	35.0%
	AGAAGGTTGAAAACAAACCT+GGG	-	chr8.1:80657342-80657361	None:intergenic	35.0%
	AGAGAATATAGATGTGACTG+TGG	+	chr8.1:80657795-80657814	MS.gene052725:CDS	35.0%
	ATTTGTGAGATCTGCAACAA+AGG	+	chr8.1:80657495-80657514	MS.gene052725:CDS	35.0%
	GAAGAGGTCATAATTTACCA+TGG	+	chr8.1:80657550-80657569	MS.gene052725:CDS	35.0%
	TCAAACTTAACTTCTGCATC+TGG	+	chr8.1:80657190-80657209	MS.gene052725:CDS	35.0%
	TCTGGTAACAGAACTGAAAT+TGG	+	chr8.1:80657229-80657248	MS.gene052725:CDS	35.0%
!!	ATGATCCATCAAGAGCATTA+GGG	+	chr8.1:80657646-80657665	MS.gene052725:CDS	35.0%
!!!	TTTTTCATTGGTTGAGTTGG+AGG	-	chr8.1:80657298-80657317	None:intergenic	35.0%
	AAAACAAACCTGGGTTACCA+GGG	-	chr8.1:80657333-80657352	None:intergenic	40.0%
	AAATCCCCTAATGCTCTTGA+TGG	-	chr8.1:80657654-80657673	None:intergenic	40.0%
	ATAGCTATAACCTCAGCATC+AGG	-	chr8.1:80657441-80657460	None:intergenic	40.0%
	GTTCTTTGCTTCAACTTCCA+TGG	-	chr8.1:80657570-80657589	None:intergenic	40.0%
	TAGCTATAACCTCAGCATCA+GGG	-	chr8.1:80657440-80657459	None:intergenic	40.0%
!	AAGCACTTTTCCAGAAAGCA+TGG	+	chr8.1:80657687-80657706	MS.gene052725:CDS	40.0%
!	TTCTCTAGTGCCACATGTTT+TGG	-	chr8.1:80657781-80657800	None:intergenic	40.0%
!!	CATGATCCATCAAGAGCATT+AGG	+	chr8.1:80657645-80657664	MS.gene052725:CDS	40.0%
!!	TGATCCATCAAGAGCATTAG+GGG	+	chr8.1:80657647-80657666	MS.gene052725:CDS	40.0%
	CTTCTTCTCACCATGCTTTC+TGG	-	chr8.1:80657700-80657719	None:intergenic	45.0%
	GAAAACAAACCTGGGTTACC+AGG	-	chr8.1:80657334-80657353	None:intergenic	45.0%
	GGATCATGATGCACACAAGT+TGG	-	chr8.1:80657633-80657652	None:intergenic	45.0%
	GGTGAAGCTAGTGTTTCTTC+TGG	+	chr8.1:80657211-80657230	MS.gene052725:CDS	45.0%
	GTTCCAACAGCAACAAGTAG+TGG	+	chr8.1:80657156-80657175	MS.gene052725:CDS	45.0%
!	AGAGCATTAGGGGATTTGAC+TGG	+	chr8.1:80657657-80657676	MS.gene052725:CDS	45.0%
!	CTTCCACTACTTGTTGCTGT+TGG	-	chr8.1:80657162-80657181	None:intergenic	45.0%
!	GAAAGCATGGTGAGAAGAAG+TGG	+	chr8.1:80657700-80657719	MS.gene052725:CDS	45.0%
!!	TGATGCACACAAGTTGGTTC+TGG	-	chr8.1:80657627-80657646	None:intergenic	45.0%
!!	TTTTTCAGACCCTGATGCTG+AGG	+	chr8.1:80657428-80657447	MS.gene052725:intron	45.0%
	ACTGTGGAACCCTCTTCTCA+AGG	+	chr8.1:80657811-80657830	MS.gene052725:CDS	50.0%
	ACAAACCTGGGTTACCAGGG+AGG	-	chr8.1:80657330-80657349	None:intergenic	55.0%
	AGAAACCTCCCTGGTAACCC+AGG	+	chr8.1:80657322-80657341	MS.gene052725:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.1	gene	80657136	80657920	80657136	ID=MS.gene052725
chr8.1	mRNA	80657136	80657920	80657136	ID=MS.gene052725.t1;Parent=MS.gene052725
chr8.1	exon	80657136	80657343	80657136	ID=MS.gene052725.t1.exon1;Parent=MS.gene052725.t1
chr8.1	CDS	80657136	80657343	80657136	ID=cds.MS.gene052725.t1;Parent=MS.gene052725.t1
chr8.1	exon	80657436	80657832	80657436	ID=MS.gene052725.t1.exon2;Parent=MS.gene052725.t1
chr8.1	CDS	80657436	80657832	80657436	ID=cds.MS.gene052725.t1;Parent=MS.gene052725.t1
chr8.1	exon	80657911	80657920	80657911	ID=MS.gene052725.t1.exon3;Parent=MS.gene052725.t1
chr8.1	CDS	80657911	80657920	80657911	ID=cds.MS.gene052725.t1;Parent=MS.gene052725.t1

Gene Sequence

>MS.gene052725

ATGGTAAAAGGTTTGATGATGTTCCAACAGCAACAAGTAGTGGAAGAAAACATGTCAAACTTAACTTCTGCATCTGGTGAAGCTAGTGTTTCTTCTGGTAACAGAACTGAAATTGGTACTAGTAGTAGTTACCCTCAAGTTCAACAACAATACTTGGTTCCTCCAACTCAACCAATGAAAAAAAAGAGAAACCTCCCTGGTAACCCAGGTTTGTTTTCAACCTTCTTTTTGTTTTGTTTTGTTTTGATAAACATGTTATTAATGGGTTTTTTTTTGTTTGGAAAATGATGTTTTTTTCAGACCCTGATGCTGAGGTTATAGCTATGTCACCAAAGAGTTTACTAGCAAAAAACAGGTTCATTTGTGAGATCTGCAACAAAGGCTTTCAAAGAGATCAAAATCTTCAACTTCACAGAAGAGGTCATAATTTACCATGGAAGTTGAAGCAAAGAACAAGTAAAGAGATTAGAAAGAAAGTTTATGTATGTCCAGAACCAACTTGTGTGCATCATGATCCATCAAGAGCATTAGGGGATTTGACTGGAATCAAGAAGCACTTTTCCAGAAAGCATGGTGAGAAGAAGTGGAAATGTGAAAAGTGTTCAAAGAAATATGCTGTTCAATCTGATTGGAAAGCTCATTCCAAAACATGTGGCACTAGAGAATATAGATGTGACTGTGGAACCCTCTTCTCAAGGTAACTAAATTAACATCAAAATATTCAATGTCATGTGAATTTTTTTAGTTCAGACATTAATTTCATTCATTTATGAAGGTGGTCATAG

Protein sequence

>MS.gene052725.t1

MVKGLMMFQQQQVVEENMSNLTSASGEASVSSGNRTEIGTSSSYPQVQQQYLVPPTQPMKKKRNLPGNPDPDAEVIAMSPKSLLAKNRFICEICNKGFQRDQNLQLHRRGHNLPWKLKQRTSKEIRKKVYVCPEPTCVHHDPSRALGDLTGIKKHFSRKHGEKKWKCEKCSKKYAVQSDWKAHSKTCGTREYRCDCGTLFSRWS