Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07860.t1 | XP_013444416.1 | 99.1 | 352 | 3 | 0 | 1 | 352 | 1 | 352 | 7.10E-191 | 676.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07860.t1 | Q5RD67 | 29.1 | 340 | 190 | 8 | 22 | 350 | 10 | 309 | 1.3e-28 | 128.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07860.t1 | A0A072TMM7 | 99.1 | 352 | 3 | 0 | 1 | 352 | 1 | 352 | 5.1e-191 | 676.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene07860 | MS.gene047200 | PPI |
| MS.gene07860 | MS.gene072058 | PPI |
| MS.gene07860 | MS.gene00617 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07860.t1 | MTR_8g019480 | 99.148 | 352 | 3 | 0 | 1 | 352 | 1 | 352 | 0.0 | 715 |
| MS.gene07860.t1 | MTR_3g104810 | 64.689 | 354 | 114 | 4 | 1 | 349 | 1 | 348 | 3.45e-157 | 444 |
| MS.gene07860.t1 | MTR_7g015870 | 46.505 | 329 | 146 | 5 | 21 | 345 | 9 | 311 | 2.28e-94 | 285 |
| MS.gene07860.t1 | MTR_7g015870 | 46.933 | 326 | 143 | 5 | 27 | 348 | 19 | 318 | 3.15e-94 | 283 |
| MS.gene07860.t1 | MTR_6g015860 | 45.075 | 335 | 154 | 6 | 21 | 351 | 5 | 313 | 9.95e-94 | 282 |
| MS.gene07860.t1 | MTR_2g032610 | 40.426 | 329 | 167 | 6 | 21 | 344 | 17 | 321 | 1.53e-81 | 251 |
| MS.gene07860.t1 | MTR_1g097890 | 26.797 | 306 | 166 | 7 | 43 | 348 | 61 | 308 | 1.16e-25 | 105 |
| MS.gene07860.t1 | MTR_8g092080 | 27.124 | 306 | 165 | 9 | 43 | 348 | 62 | 309 | 2.11e-21 | 93.6 |
| MS.gene07860.t1 | MTR_5g073040 | 27.331 | 311 | 174 | 7 | 47 | 349 | 53 | 319 | 4.86e-21 | 92.8 |
| MS.gene07860.t1 | MTR_5g073040 | 30.928 | 194 | 101 | 6 | 41 | 218 | 135 | 311 | 3.29e-12 | 67.0 |
| MS.gene07860.t1 | MTR_4g114320 | 24.921 | 317 | 195 | 8 | 34 | 348 | 123 | 398 | 9.88e-21 | 92.8 |
| MS.gene07860.t1 | MTR_6g034515 | 27.181 | 298 | 157 | 12 | 45 | 330 | 79 | 328 | 3.37e-19 | 87.8 |
| MS.gene07860.t1 | MTR_6g034515 | 30.857 | 175 | 114 | 5 | 44 | 218 | 171 | 338 | 7.07e-11 | 63.2 |
| MS.gene07860.t1 | MTR_4g012850 | 23.962 | 313 | 201 | 7 | 40 | 350 | 21 | 298 | 3.04e-17 | 81.6 |
| MS.gene07860.t1 | MTR_8g098240 | 26.101 | 318 | 185 | 10 | 27 | 341 | 314 | 584 | 2.82e-16 | 80.5 |
| MS.gene07860.t1 | MTR_1g012030 | 24.324 | 296 | 182 | 8 | 49 | 343 | 155 | 409 | 5.55e-16 | 79.0 |
| MS.gene07860.t1 | MTR_1g012030 | 25.581 | 215 | 120 | 4 | 132 | 346 | 140 | 314 | 7.92e-12 | 66.2 |
| MS.gene07860.t1 | MTR_4g088545 | 24.290 | 317 | 199 | 11 | 37 | 343 | 14 | 299 | 3.43e-15 | 75.5 |
| MS.gene07860.t1 | MTR_7g068340 | 26.794 | 209 | 132 | 5 | 35 | 241 | 187 | 376 | 9.28e-15 | 75.1 |
| MS.gene07860.t1 | MTR_7g064980 | 24.828 | 290 | 179 | 7 | 62 | 348 | 65 | 318 | 1.84e-14 | 73.6 |
| MS.gene07860.t1 | MTR_7g109135 | 23.432 | 303 | 189 | 8 | 49 | 343 | 38 | 305 | 2.31e-14 | 73.6 |
| MS.gene07860.t1 | MTR_8g076760 | 24.116 | 311 | 199 | 9 | 47 | 347 | 28 | 311 | 1.69e-13 | 70.9 |
| MS.gene07860.t1 | MTR_2g102010 | 25.818 | 275 | 166 | 7 | 48 | 320 | 32 | 270 | 2.28e-13 | 70.1 |
| MS.gene07860.t1 | MTR_8g027975 | 30.846 | 201 | 124 | 7 | 35 | 221 | 154 | 353 | 3.87e-13 | 70.1 |
| MS.gene07860.t1 | MTR_8g027975 | 23.929 | 280 | 174 | 6 | 77 | 343 | 99 | 352 | 7.13e-12 | 66.2 |
| MS.gene07860.t1 | MTR_4g016730 | 28.272 | 191 | 102 | 5 | 35 | 221 | 196 | 355 | 4.98e-13 | 69.7 |
| MS.gene07860.t1 | MTR_5g009500 | 29.500 | 200 | 126 | 7 | 35 | 221 | 141 | 338 | 2.71e-12 | 67.4 |
| MS.gene07860.t1 | MTR_5g009500 | 25.180 | 278 | 171 | 6 | 75 | 341 | 84 | 335 | 2.74e-11 | 64.3 |
| MS.gene07860.t1 | MTR_5g055410 | 29.756 | 205 | 112 | 8 | 49 | 238 | 135 | 322 | 6.29e-12 | 66.2 |
| MS.gene07860.t1 | MTR_5g055410 | 25.828 | 302 | 183 | 9 | 49 | 343 | 37 | 304 | 1.12e-11 | 65.5 |
| MS.gene07860.t1 | MTR_1g087840 | 24.759 | 311 | 175 | 10 | 49 | 343 | 35 | 302 | 1.13e-11 | 65.5 |
| MS.gene07860.t1 | MTR_2g098070 | 30.921 | 152 | 85 | 4 | 74 | 223 | 221 | 354 | 1.46e-11 | 65.5 |
| MS.gene07860.t1 | MTR_2g436930 | 23.929 | 280 | 168 | 9 | 73 | 339 | 72 | 319 | 1.63e-11 | 65.1 |
| MS.gene07860.t1 | MTR_2g436930 | 28.191 | 188 | 121 | 7 | 41 | 216 | 134 | 319 | 6.88e-11 | 63.2 |
| MS.gene07860.t1 | MTR_3g107510 | 26.484 | 219 | 113 | 5 | 124 | 342 | 123 | 293 | 1.74e-11 | 65.5 |
| MS.gene07860.t1 | MTR_7g084400 | 29.319 | 191 | 114 | 5 | 34 | 219 | 309 | 483 | 2.38e-11 | 65.1 |
| MS.gene07860.t1 | MTR_4g088545 | 24.336 | 226 | 136 | 6 | 120 | 341 | 1 | 195 | 2.95e-11 | 63.2 |
| MS.gene07860.t1 | MTR_4g119770 | 29.319 | 191 | 113 | 8 | 40 | 214 | 134 | 318 | 3.21e-11 | 63.9 |
| MS.gene07860.t1 | MTR_4g057850 | 24.830 | 294 | 161 | 10 | 44 | 322 | 33 | 281 | 3.59e-11 | 63.9 |
| MS.gene07860.t1 | MTR_2g436930 | 28.191 | 188 | 121 | 7 | 41 | 216 | 44 | 229 | 3.95e-11 | 62.8 |
| MS.gene07860.t1 | MTR_3g025800 | 26.103 | 272 | 160 | 6 | 33 | 286 | 28 | 276 | 4.92e-11 | 63.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07860.t1 | AT1G72820 | 72.727 | 352 | 84 | 5 | 1 | 349 | 1 | 343 | 6.20e-180 | 502 |
| MS.gene07860.t1 | AT1G72820 | 72.727 | 352 | 84 | 5 | 1 | 349 | 1 | 343 | 6.20e-180 | 502 |
| MS.gene07860.t1 | AT5G26200 | 62.242 | 339 | 107 | 3 | 15 | 349 | 21 | 342 | 5.53e-149 | 424 |
| MS.gene07860.t1 | AT5G15640 | 47.416 | 329 | 145 | 6 | 21 | 345 | 16 | 320 | 9.45e-95 | 285 |
| MS.gene07860.t1 | AT5G15640 | 47.256 | 328 | 144 | 7 | 22 | 345 | 11 | 313 | 5.94e-92 | 278 |
| MS.gene07860.t1 | AT1G34065 | 25.974 | 308 | 169 | 8 | 43 | 349 | 92 | 341 | 2.93e-22 | 96.7 |
| MS.gene07860.t1 | AT1G34065 | 25.974 | 308 | 169 | 8 | 43 | 349 | 85 | 334 | 2.98e-22 | 96.3 |
| MS.gene07860.t1 | AT4G11440 | 26.100 | 341 | 209 | 9 | 3 | 341 | 301 | 600 | 9.82e-22 | 97.1 |
| MS.gene07860.t1 | AT4G11440 | 26.100 | 341 | 209 | 9 | 3 | 341 | 301 | 600 | 9.82e-22 | 97.1 |
| MS.gene07860.t1 | AT2G37890 | 27.208 | 283 | 160 | 9 | 68 | 343 | 88 | 331 | 1.60e-19 | 88.6 |
| MS.gene07860.t1 | AT1G07030 | 27.273 | 319 | 185 | 7 | 31 | 343 | 38 | 315 | 1.22e-18 | 85.9 |
| MS.gene07860.t1 | AT4G39460 | 25.817 | 306 | 169 | 9 | 43 | 348 | 68 | 315 | 1.29e-18 | 85.9 |
| MS.gene07860.t1 | AT4G39460 | 25.817 | 306 | 169 | 9 | 43 | 348 | 68 | 315 | 1.29e-18 | 85.9 |
| MS.gene07860.t1 | AT4G39460 | 25.817 | 306 | 169 | 9 | 43 | 348 | 68 | 315 | 1.29e-18 | 85.9 |
| MS.gene07860.t1 | AT2G30160 | 27.815 | 302 | 176 | 9 | 47 | 343 | 56 | 320 | 7.10e-18 | 84.0 |
| MS.gene07860.t1 | AT2G30160 | 31.606 | 193 | 113 | 5 | 37 | 218 | 134 | 318 | 1.75e-13 | 70.9 |
| MS.gene07860.t1 | AT2G30160 | 26.066 | 211 | 116 | 5 | 132 | 342 | 43 | 213 | 3.10e-12 | 67.4 |
| MS.gene07860.t1 | AT3G53940 | 26.354 | 277 | 160 | 7 | 73 | 343 | 121 | 359 | 8.87e-16 | 78.2 |
| MS.gene07860.t1 | AT3G20240 | 25.914 | 301 | 172 | 8 | 34 | 320 | 52 | 315 | 6.51e-15 | 75.5 |
| MS.gene07860.t1 | AT3G20240 | 29.444 | 180 | 105 | 6 | 41 | 214 | 169 | 332 | 6.22e-13 | 69.3 |
| MS.gene07860.t1 | AT4G32400 | 23.585 | 318 | 200 | 9 | 34 | 349 | 114 | 390 | 9.95e-15 | 75.1 |
| MS.gene07860.t1 | AT1G79900 | 24.746 | 295 | 178 | 8 | 48 | 342 | 31 | 281 | 6.68e-14 | 71.6 |
| MS.gene07860.t1 | AT1G79900 | 29.050 | 179 | 112 | 3 | 44 | 222 | 121 | 284 | 1.04e-11 | 65.5 |
| MS.gene07860.t1 | AT5G01340 | 28.041 | 296 | 150 | 12 | 44 | 322 | 28 | 277 | 6.22e-13 | 68.9 |
| MS.gene07860.t1 | AT1G25380 | 25.243 | 309 | 177 | 9 | 49 | 343 | 37 | 305 | 1.18e-12 | 68.6 |
| MS.gene07860.t1 | AT1G25380 | 25.243 | 309 | 177 | 9 | 49 | 343 | 37 | 305 | 1.18e-12 | 68.6 |
| MS.gene07860.t1 | AT5G48970 | 23.782 | 349 | 207 | 10 | 19 | 339 | 12 | 329 | 1.44e-12 | 68.2 |
| MS.gene07860.t1 | AT3G51870 | 25.926 | 189 | 119 | 3 | 35 | 221 | 191 | 360 | 2.28e-12 | 67.8 |
| MS.gene07860.t1 | AT5G66380 | 24.430 | 307 | 196 | 9 | 45 | 343 | 22 | 300 | 2.55e-12 | 67.4 |
| MS.gene07860.t1 | AT5G51050 | 23.750 | 320 | 201 | 9 | 25 | 342 | 215 | 493 | 4.12e-12 | 67.4 |
| MS.gene07860.t1 | AT5G48970 | 24.460 | 278 | 174 | 7 | 72 | 339 | 7 | 258 | 4.44e-12 | 66.2 |
| MS.gene07860.t1 | AT5G48970 | 27.193 | 228 | 120 | 9 | 11 | 214 | 51 | 256 | 3.28e-11 | 63.5 |
| MS.gene07860.t1 | AT2G47490 | 25.000 | 308 | 177 | 10 | 49 | 343 | 33 | 299 | 8.46e-12 | 65.9 |
| MS.gene07860.t1 | AT1G74240 | 24.611 | 321 | 182 | 13 | 29 | 322 | 27 | 314 | 1.69e-11 | 65.1 |
| MS.gene07860.t1 | AT1G14560 | 22.185 | 302 | 191 | 7 | 51 | 341 | 49 | 317 | 3.13e-11 | 64.3 |
| MS.gene07860.t1 | AT4G01100 | 28.436 | 211 | 134 | 8 | 35 | 231 | 95 | 302 | 3.31e-11 | 63.9 |
| MS.gene07860.t1 | AT3G54110 | 25.902 | 305 | 175 | 10 | 49 | 342 | 31 | 295 | 3.50e-11 | 63.9 |
| MS.gene07860.t1 | AT1G74240 | 25.168 | 298 | 168 | 12 | 47 | 322 | 2 | 266 | 5.36e-11 | 63.5 |
| MS.gene07860.t1 | AT4G01100 | 28.436 | 211 | 134 | 8 | 35 | 231 | 145 | 352 | 5.40e-11 | 63.5 |
| MS.gene07860.t1 | AT4G01100 | 28.436 | 211 | 134 | 8 | 35 | 231 | 159 | 366 | 7.66e-11 | 63.2 |
| MS.gene07860.t1 | AT5G46800 | 26.606 | 218 | 122 | 7 | 132 | 341 | 109 | 296 | 7.71e-11 | 62.8 |
| MS.gene07860.t1 | AT5G46800 | 26.606 | 218 | 122 | 7 | 132 | 341 | 109 | 296 | 7.71e-11 | 62.8 |
| MS.gene07860.t1 | AT5G46800 | 26.606 | 218 | 122 | 7 | 132 | 341 | 109 | 296 | 7.71e-11 | 62.8 |
Find 95 sgRNAs with CRISPR-Local
Find 114 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGGGGTTTGTATAGAGGTTT+TGG | 0.169259 | 8.3:-71493417 | MS.gene07860:CDS |
| CTGTTGCACAAAGGATGGTT+TGG | 0.306479 | 8.3:-71493341 | MS.gene07860:CDS |
| TGGGTTTGGTACTTCGTTGA+TGG | 0.323359 | 8.3:-71493733 | MS.gene07860:CDS |
| GGAACTGCTACGGTTGGTTT+TGG | 0.337433 | 8.3:-71493651 | MS.gene07860:CDS |
| GATGCAGACGGTTAGAAGTT+TGG | 0.354056 | 8.3:-71493088 | MS.gene07860:CDS |
| GCAATTGCTAATGCGGTTGC+TGG | 0.368970 | 8.3:-71493606 | MS.gene07860:CDS |
| TGTTGCACAAAGGATGGTTT+GGG | 0.377065 | 8.3:-71493340 | MS.gene07860:CDS |
| CCCGGCTAGGGCTTTGTATA+TGG | 0.377308 | 8.3:-71493703 | MS.gene07860:CDS |
| TCGTCTGTAAGATATGTTAA+TGG | 0.389810 | 8.3:-71493480 | MS.gene07860:CDS |
| TAAAACCTCTTGATTCTTTG+CGG | 0.394234 | 8.3:+71492954 | None:intergenic |
| GGTTGGATGGCTTGTTATAG+AGG | 0.399503 | 8.3:-71493054 | MS.gene07860:CDS |
| AGAGCGTTGTATCGTGGGTT+TGG | 0.403147 | 8.3:-71493747 | MS.gene07860:CDS |
| ATGGCTTGTTATAGAGGATT+GGG | 0.407744 | 8.3:-71493048 | MS.gene07860:CDS |
| AGGATGGTTTGGGGTGGAGT+TGG | 0.412562 | 8.3:-71493330 | MS.gene07860:CDS |
| ATAGAGGATTGGGACCTAGA+TGG | 0.418086 | 8.3:-71493038 | MS.gene07860:CDS |
| GGTCCTAGGGGTTTGTATAG+AGG | 0.420101 | 8.3:-71493423 | MS.gene07860:CDS |
| TGCTTTGATTACTATGCCAT+TGG | 0.422067 | 8.3:-71493175 | MS.gene07860:CDS |
| TACCTTGAACCATCAACCTT+TGG | 0.422147 | 8.3:+71493529 | None:intergenic |
| ACAAGGCTGCAGGTCTTAGA+TGG | 0.426779 | 8.3:-71493141 | MS.gene07860:CDS |
| GATGGCTTGTTATAGAGGAT+TGG | 0.434742 | 8.3:-71493049 | MS.gene07860:CDS |
| TGAGGAAGAGGTAGAGTCAG+AGG | 0.443657 | 8.3:-71493967 | MS.gene07860:CDS |
| GACGGTTAGAAGTTTGGTTA+AGG | 0.449500 | 8.3:-71493082 | MS.gene07860:CDS |
| GGTTAAGGAAGGTGGTTGGA+TGG | 0.449945 | 8.3:-71493067 | MS.gene07860:CDS |
| GGGTTTGGTACTTCGTTGAT+GGG | 0.450005 | 8.3:-71493732 | MS.gene07860:CDS |
| TTGTGAGAACGGATGGTCCT+AGG | 0.450225 | 8.3:-71493437 | MS.gene07860:CDS |
| TTGTAATTCAAGTGATCCTA+AGG | 0.453755 | 8.3:-71493505 | MS.gene07860:CDS |
| TGTTGTGAGCCAAAGGTTGA+TGG | 0.459063 | 8.3:-71493538 | MS.gene07860:CDS |
| AGAAGTTTGGTTAAGGAAGG+TGG | 0.466995 | 8.3:-71493075 | MS.gene07860:CDS |
| CTGTTGTTGTGTTGAAAACT+AGG | 0.469626 | 8.3:-71493839 | MS.gene07860:CDS |
| ATGCGCCTTCAAATGCAGTA+TGG | 0.472358 | 8.3:-71493377 | MS.gene07860:CDS |
| GGTTTAGAGCGTTGTATCGT+GGG | 0.474518 | 8.3:-71493752 | MS.gene07860:CDS |
| AGGAAAATTGTGAGAACGGA+TGG | 0.476248 | 8.3:-71493444 | MS.gene07860:CDS |
| GTTAGAAGTTTGGTTAAGGA+AGG | 0.488369 | 8.3:-71493078 | MS.gene07860:CDS |
| GCCCACCATACTGCATTTGA+AGG | 0.489267 | 8.3:+71493372 | None:intergenic |
| CTTTGGCTCACAACATCAAT+CGG | 0.491438 | 8.3:+71493546 | None:intergenic |
| TTCGTTGATGGGTACAATCC+CGG | 0.494552 | 8.3:-71493721 | MS.gene07860:CDS |
| AAAACTGTTATGGCAGTTCA+GGG | 0.494556 | 8.3:-71493228 | MS.gene07860:CDS |
| AGCCAAAGGTTGATGGTTCA+AGG | 0.498610 | 8.3:-71493531 | MS.gene07860:CDS |
| GTCTTAGATGGCGATGAGAA+TGG | 0.502262 | 8.3:-71493129 | MS.gene07860:CDS |
| ATGTGCCAGCTGAAATTGAT+TGG | 0.503156 | 8.3:-71493935 | MS.gene07860:CDS |
| GCCATATACAAAGCCCTAGC+CGG | 0.504895 | 8.3:+71493702 | None:intergenic |
| ATTTGCCAATCAATTTCAGC+TGG | 0.506380 | 8.3:+71493930 | None:intergenic |
| TGTGAGAACGGATGGTCCTA+GGG | 0.509331 | 8.3:-71493436 | MS.gene07860:CDS |
| TAGAGGATTGGGACCTAGAT+GGG | 0.510090 | 8.3:-71493037 | MS.gene07860:CDS |
| TGATGGGTACAATCCCGGCT+AGG | 0.512863 | 8.3:-71493716 | MS.gene07860:CDS |
| ACTTGAGTAACAAGAAAGGC+GGG | 0.515766 | 8.3:-71493302 | MS.gene07860:CDS |
| TTTGAGTGTGAATGAGGAAG+AGG | 0.516208 | 8.3:-71493979 | MS.gene07860:CDS |
| GGAATCAGTGCAGCAATGGC+AGG | 0.517764 | 8.3:-71493207 | MS.gene07860:CDS |
| ATATTCTGTTGCACAAAGGA+TGG | 0.521296 | 8.3:-71493346 | MS.gene07860:CDS |
| GATGGGTACAATCCCGGCTA+GGG | 0.527294 | 8.3:-71493715 | MS.gene07860:CDS |
| CTTTAGCAGCTCAGCTTGTG+TGG | 0.529736 | 8.3:-71493572 | MS.gene07860:CDS |
| GTTTGGTTAAGGAAGGTGGT+TGG | 0.535300 | 8.3:-71493071 | MS.gene07860:CDS |
| TACAATCAAGACAAGGCTGC+AGG | 0.537518 | 8.3:-71493151 | MS.gene07860:CDS |
| GGGTTTAGAGCGTTGTATCG+TGG | 0.538900 | 8.3:-71493753 | MS.gene07860:CDS |
| CCATATACAAAGCCCTAGCC+GGG | 0.540476 | 8.3:+71493703 | None:intergenic |
| AACGACATTGAAGCCCATCT+AGG | 0.543587 | 8.3:+71493024 | None:intergenic |
| AACGTAGGAACTGCTACGGT+TGG | 0.544642 | 8.3:-71493657 | MS.gene07860:CDS |
| TATCTTACAGACGAACCCTT+AGG | 0.545681 | 8.3:+71493489 | None:intergenic |
| CGCCTTCAAATGCAGTATGG+TGG | 0.550964 | 8.3:-71493374 | MS.gene07860:CDS |
| GGAAGTCACAACAACAATGG+AGG | 0.554499 | 8.3:-71493276 | MS.gene07860:CDS |
| TTAGCAATTGCAGCAGCTGT+CGG | 0.556204 | 8.3:+71493618 | None:intergenic |
| GCCTTCAAATGCAGTATGGT+GGG | 0.558047 | 8.3:-71493373 | MS.gene07860:CDS |
| CTTGTCTTGATTGTATCCAA+TGG | 0.562754 | 8.3:+71493159 | None:intergenic |
| TTTGTATATGGCGGCGCTTG+AGG | 0.563477 | 8.3:-71493691 | MS.gene07860:CDS |
| GGCGATGAGAATGGCCGTCG+TGG | 0.574613 | 8.3:-71493120 | MS.gene07860:CDS |
| TCAGGGAATCAGTGCAGCAA+TGG | 0.574833 | 8.3:-71493211 | MS.gene07860:CDS |
| AGCTGCTGCAATTGCTAATG+CGG | 0.576587 | 8.3:-71493613 | MS.gene07860:CDS |
| AGTAACAAGAAAGGCGGGGA+GGG | 0.585042 | 8.3:-71493297 | MS.gene07860:CDS |
| AGGGGCTAATGTGTTGAGGC+CGG | 0.590691 | 8.3:-71493256 | MS.gene07860:CDS |
| GAAGTCACAACAACAATGGA+GGG | 0.599876 | 8.3:-71493275 | MS.gene07860:CDS |
| TACTTGAGTAACAAGAAAGG+CGG | 0.600908 | 8.3:-71493303 | MS.gene07860:CDS |
| TGTAATTCAAGTGATCCTAA+GGG | 0.603205 | 8.3:-71493504 | MS.gene07860:CDS |
| TATTACTTGAGTAACAAGAA+AGG | 0.608260 | 8.3:-71493306 | MS.gene07860:CDS |
| GTTGCACAAAGGATGGTTTG+GGG | 0.609426 | 8.3:-71493339 | MS.gene07860:CDS |
| CGATTGATGTTGTGAGCCAA+AGG | 0.611253 | 8.3:-71493545 | MS.gene07860:CDS |
| GAGGGAAGTCACAACAACAA+TGG | 0.611518 | 8.3:-71493279 | MS.gene07860:CDS |
| CTTCATATTCTGTTGCACAA+AGG | 0.611809 | 8.3:-71493350 | MS.gene07860:CDS |
| AATGGATTTGAGTGTGAATG+AGG | 0.612137 | 8.3:-71493985 | MS.gene07860:CDS |
| GCTTTCCGCAAAGAATCAAG+AGG | 0.618949 | 8.3:-71492959 | MS.gene07860:CDS |
| AAACCTCTATACAAACCCCT+AGG | 0.620850 | 8.3:+71493420 | None:intergenic |
| ATCAGTGCAGCAATGGCAGG+AGG | 0.625647 | 8.3:-71493204 | MS.gene07860:CDS |
| GCACAAAGGATGGTTTGGGG+TGG | 0.629155 | 8.3:-71493336 | MS.gene07860:CDS |
| GGCTAGGGCTTTGTATATGG+CGG | 0.640912 | 8.3:-71493700 | MS.gene07860:CDS |
| ATGGAGGGGCTAATGTGTTG+AGG | 0.647603 | 8.3:-71493260 | MS.gene07860:CDS |
| CTAACCGTCTGCATCACTGT+TGG | 0.653039 | 8.3:+71493096 | None:intergenic |
| GCATCACTGTTGGTCCACGA+CGG | 0.662402 | 8.3:+71493106 | None:intergenic |
| CTTGAGTAACAAGAAAGGCG+GGG | 0.665534 | 8.3:-71493301 | MS.gene07860:CDS |
| GAGTAACGTAGGAACTGCTA+CGG | 0.669145 | 8.3:-71493661 | MS.gene07860:CDS |
| GAGTAACAAGAAAGGCGGGG+AGG | 0.670261 | 8.3:-71493298 | MS.gene07860:CDS |
| GAGGTTACTAAGAGTAACGT+AGG | 0.677918 | 8.3:-71493672 | MS.gene07860:CDS |
| GTGAGAACGGATGGTCCTAG+GGG | 0.680233 | 8.3:-71493435 | MS.gene07860:CDS |
| CATTGGATACAATCAAGACA+AGG | 0.683298 | 8.3:-71493158 | MS.gene07860:CDS |
| CAGAAAACAAAGCAGCACCG+AGG | 0.690959 | 8.3:+71493883 | None:intergenic |
| TGGACCAACAGTGATGCAGA+CGG | 0.713938 | 8.3:-71493100 | MS.gene07860:CDS |
| AAGTCACAACAACAATGGAG+GGG | 0.716540 | 8.3:-71493274 | MS.gene07860:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TATTACTTGAGTAACAAGAA+AGG | - | chr8.3:71493626-71493645 | MS.gene07860:CDS | 25.0% |
| !!! | TTAATGGTATTGATGCTTTT+AGG | - | chr8.3:71493468-71493487 | MS.gene07860:CDS | 25.0% |
| !!! | TTTTAGGAAAATTGTGAGAA+CGG | - | chr8.3:71493484-71493503 | MS.gene07860:CDS | 25.0% |
| TAAAACCTCTTGATTCTTTG+CGG | + | chr8.3:71493981-71494000 | None:intergenic | 30.0% | |
| TCGTCTGTAAGATATGTTAA+TGG | - | chr8.3:71493452-71493471 | MS.gene07860:CDS | 30.0% | |
| TGTAATTCAAGTGATCCTAA+GGG | - | chr8.3:71493428-71493447 | MS.gene07860:CDS | 30.0% | |
| TTGTAATTCAAGTGATCCTA+AGG | - | chr8.3:71493427-71493446 | MS.gene07860:CDS | 30.0% | |
| ! | AAATCCAAGTTTTTCTTCCT+CGG | - | chr8.3:71493032-71493051 | MS.gene07860:CDS | 30.0% |
| !! | CAACCTATGAGCTTTTAAAA+CGG | - | chr8.3:71493951-71493970 | MS.gene07860:CDS | 30.0% |
| AAAAACTGTTATGGCAGTTC+AGG | - | chr8.3:71493703-71493722 | MS.gene07860:CDS | 35.0% | |
| AAAACTGTTATGGCAGTTCA+GGG | - | chr8.3:71493704-71493723 | MS.gene07860:CDS | 35.0% | |
| ATATTCTGTTGCACAAAGGA+TGG | - | chr8.3:71493586-71493605 | MS.gene07860:CDS | 35.0% | |
| ATTTGCCAATCAATTTCAGC+TGG | + | chr8.3:71493005-71493024 | None:intergenic | 35.0% | |
| CATTGGATACAATCAAGACA+AGG | - | chr8.3:71493774-71493793 | MS.gene07860:CDS | 35.0% | |
| CTGTTGTTGTGTTGAAAACT+AGG | - | chr8.3:71493093-71493112 | MS.gene07860:CDS | 35.0% | |
| CTTCATATTCTGTTGCACAA+AGG | - | chr8.3:71493582-71493601 | MS.gene07860:CDS | 35.0% | |
| CTTGTCTTGATTGTATCCAA+TGG | + | chr8.3:71493776-71493795 | None:intergenic | 35.0% | |
| GTTAGAAGTTTGGTTAAGGA+AGG | - | chr8.3:71493854-71493873 | MS.gene07860:CDS | 35.0% | |
| TACTTGAGTAACAAGAAAGG+CGG | - | chr8.3:71493629-71493648 | MS.gene07860:CDS | 35.0% | |
| TGCTTTGATTACTATGCCAT+TGG | - | chr8.3:71493757-71493776 | MS.gene07860:CDS | 35.0% | |
| ! | GCCATAACAGTTTTTGTATC+CGG | + | chr8.3:71493698-71493717 | None:intergenic | 35.0% |
| !! | ACTGCGTTTTCGTTGATTAA+GGG | - | chr8.3:71493152-71493171 | MS.gene07860:CDS | 35.0% |
| !! | ATGGCTTGTTATAGAGGATT+GGG | - | chr8.3:71493884-71493903 | MS.gene07860:CDS | 35.0% |
| !! | CTAGTTTTCAACACAACAAC+AGG | + | chr8.3:71493095-71493114 | None:intergenic | 35.0% |
| !!! | AAGCCGTTTTAAAAGCTCAT+AGG | + | chr8.3:71493957-71493976 | None:intergenic | 35.0% |
| !!! | GTATAGAGGTTTTGGGATAT+CGG | - | chr8.3:71493523-71493542 | MS.gene07860:CDS | 35.0% |
| AAACCTCTATACAAACCCCT+AGG | + | chr8.3:71493515-71493534 | None:intergenic | 40.0% | |
| AAGTCACAACAACAATGGAG+GGG | - | chr8.3:71493658-71493677 | MS.gene07860:CDS | 40.0% | |
| ACTTGAGTAACAAGAAAGGC+GGG | - | chr8.3:71493630-71493649 | MS.gene07860:CDS | 40.0% | |
| AGAAGTTTGGTTAAGGAAGG+TGG | - | chr8.3:71493857-71493876 | MS.gene07860:CDS | 40.0% | |
| AGGAAAATTGTGAGAACGGA+TGG | - | chr8.3:71493488-71493507 | MS.gene07860:CDS | 40.0% | |
| ATGTGCCAGCTGAAATTGAT+TGG | - | chr8.3:71492997-71493016 | MS.gene07860:CDS | 40.0% | |
| GAAGTCACAACAACAATGGA+GGG | - | chr8.3:71493657-71493676 | MS.gene07860:CDS | 40.0% | |
| GACGGTTAGAAGTTTGGTTA+AGG | - | chr8.3:71493850-71493869 | MS.gene07860:CDS | 40.0% | |
| GAGGTTACTAAGAGTAACGT+AGG | - | chr8.3:71493260-71493279 | MS.gene07860:CDS | 40.0% | |
| GCCGGATACAAAAACTGTTA+TGG | - | chr8.3:71493694-71493713 | MS.gene07860:CDS | 40.0% | |
| TACCTTGAACCATCAACCTT+TGG | + | chr8.3:71493406-71493425 | None:intergenic | 40.0% | |
| TATCTTACAGACGAACCCTT+AGG | + | chr8.3:71493446-71493465 | None:intergenic | 40.0% | |
| TGTTGCACAAAGGATGGTTT+GGG | - | chr8.3:71493592-71493611 | MS.gene07860:CDS | 40.0% | |
| TTTGAGTGTGAATGAGGAAG+AGG | - | chr8.3:71492953-71492972 | MS.gene07860:CDS | 40.0% | |
| ! | AGGGGTTTGTATAGAGGTTT+TGG | - | chr8.3:71493515-71493534 | MS.gene07860:CDS | 40.0% |
| ! | CTTTGGCTCACAACATCAAT+CGG | + | chr8.3:71493389-71493408 | None:intergenic | 40.0% |
| ! | GGGGTTTGTATAGAGGTTTT+GGG | - | chr8.3:71493516-71493535 | MS.gene07860:CDS | 40.0% |
| !! | CTGCGTTTTCGTTGATTAAG+GGG | - | chr8.3:71493153-71493172 | MS.gene07860:CDS | 40.0% |
| !! | GACTGCGTTTTCGTTGATTA+AGG | - | chr8.3:71493151-71493170 | MS.gene07860:CDS | 40.0% |
| !! | GATGGCTTGTTATAGAGGAT+TGG | - | chr8.3:71493883-71493902 | MS.gene07860:CDS | 40.0% |
| !! | TGCGTTTTCGTTGATTAAGG+GGG | - | chr8.3:71493154-71493173 | MS.gene07860:CDS | 40.0% |
| !! | TTTTCGTTGATTAAGGGGGA+GGG | - | chr8.3:71493158-71493177 | MS.gene07860:CDS | 40.0% |
| AACGACATTGAAGCCCATCT+AGG | + | chr8.3:71493911-71493930 | None:intergenic | 45.0% | |
| AGCTGCTGCAATTGCTAATG+CGG | - | chr8.3:71493319-71493338 | MS.gene07860:CDS | 45.0% | |
| ATAGAGGATTGGGACCTAGA+TGG | - | chr8.3:71493894-71493913 | MS.gene07860:CDS | 45.0% | |
| ATGCGCCTTCAAATGCAGTA+TGG | - | chr8.3:71493555-71493574 | MS.gene07860:CDS | 45.0% | |
| CGATTGATGTTGTGAGCCAA+AGG | - | chr8.3:71493387-71493406 | MS.gene07860:CDS | 45.0% | |
| CTGTTGCACAAAGGATGGTT+TGG | - | chr8.3:71493591-71493610 | MS.gene07860:CDS | 45.0% | |
| CTTGAGTAACAAGAAAGGCG+GGG | - | chr8.3:71493631-71493650 | MS.gene07860:CDS | 45.0% | |
| GAGGGAAGTCACAACAACAA+TGG | - | chr8.3:71493653-71493672 | MS.gene07860:CDS | 45.0% | |
| GAGTAACGTAGGAACTGCTA+CGG | - | chr8.3:71493271-71493290 | MS.gene07860:CDS | 45.0% | |
| GATGCAGACGGTTAGAAGTT+TGG | - | chr8.3:71493844-71493863 | MS.gene07860:CDS | 45.0% | |
| GCCTTCAAATGCAGTATGGT+GGG | - | chr8.3:71493559-71493578 | MS.gene07860:CDS | 45.0% | |
| GCTTTCCGCAAAGAATCAAG+AGG | - | chr8.3:71493973-71493992 | MS.gene07860:CDS | 45.0% | |
| GGAAGTCACAACAACAATGG+AGG | - | chr8.3:71493656-71493675 | MS.gene07860:CDS | 45.0% | |
| GTCTTAGATGGCGATGAGAA+TGG | - | chr8.3:71493803-71493822 | MS.gene07860:CDS | 45.0% | |
| GTTGCACAAAGGATGGTTTG+GGG | - | chr8.3:71493593-71493612 | MS.gene07860:CDS | 45.0% | |
| GTTTGGTTAAGGAAGGTGGT+TGG | - | chr8.3:71493861-71493880 | MS.gene07860:CDS | 45.0% | |
| TACAATCAAGACAAGGCTGC+AGG | - | chr8.3:71493781-71493800 | MS.gene07860:CDS | 45.0% | |
| TAGAGGATTGGGACCTAGAT+GGG | - | chr8.3:71493895-71493914 | MS.gene07860:CDS | 45.0% | |
| TGTTGTGAGCCAAAGGTTGA+TGG | - | chr8.3:71493394-71493413 | MS.gene07860:CDS | 45.0% | |
| TTAGCAATTGCAGCAGCTGT+CGG | + | chr8.3:71493317-71493336 | None:intergenic | 45.0% | |
| ! | AGCACCGAGGAAGAAAAACT+TGG | + | chr8.3:71493039-71493058 | None:intergenic | 45.0% |
| ! | GGGTTTGGTACTTCGTTGAT+GGG | - | chr8.3:71493200-71493219 | MS.gene07860:CDS | 45.0% |
| ! | TGGGTTTGGTACTTCGTTGA+TGG | - | chr8.3:71493199-71493218 | MS.gene07860:CDS | 45.0% |
| ! | TTCGTTGATGGGTACAATCC+CGG | - | chr8.3:71493211-71493230 | MS.gene07860:CDS | 45.0% |
| ! | TTTCGTTGATTAAGGGGGAG+GGG | - | chr8.3:71493159-71493178 | MS.gene07860:CDS | 45.0% |
| !! | AGCCAAAGGTTGATGGTTCA+AGG | - | chr8.3:71493401-71493420 | MS.gene07860:CDS | 45.0% |
| !! | GGTTGGATGGCTTGTTATAG+AGG | - | chr8.3:71493878-71493897 | MS.gene07860:CDS | 45.0% |
| !! | GGTTTAGAGCGTTGTATCGT+GGG | - | chr8.3:71493180-71493199 | MS.gene07860:CDS | 45.0% |
| !! | GTTTTCGTTGATTAAGGGGG+AGG | - | chr8.3:71493157-71493176 | MS.gene07860:CDS | 45.0% |
| AACGTAGGAACTGCTACGGT+TGG | - | chr8.3:71493275-71493294 | MS.gene07860:CDS | 50.0% | |
| ACAAGGCTGCAGGTCTTAGA+TGG | - | chr8.3:71493791-71493810 | MS.gene07860:CDS | 50.0% | |
| AGTAACAAGAAAGGCGGGGA+GGG | - | chr8.3:71493635-71493654 | MS.gene07860:CDS | 50.0% | |
| ATGGAGGGGCTAATGTGTTG+AGG | - | chr8.3:71493672-71493691 | MS.gene07860:CDS | 50.0% | |
| CAGAAAACAAAGCAGCACCG+AGG | + | chr8.3:71493052-71493071 | None:intergenic | 50.0% | |
| CCATATACAAAGCCCTAGCC+GGG | + | chr8.3:71493232-71493251 | None:intergenic | 50.0% | |
| CGCCTTCAAATGCAGTATGG+TGG | - | chr8.3:71493558-71493577 | MS.gene07860:CDS | 50.0% | |
| CTAACCGTCTGCATCACTGT+TGG | + | chr8.3:71493839-71493858 | None:intergenic | 50.0% | |
| CTTTAGCAGCTCAGCTTGTG+TGG | - | chr8.3:71493360-71493379 | MS.gene07860:CDS | 50.0% | |
| GCAATTGCTAATGCGGTTGC+TGG | - | chr8.3:71493326-71493345 | MS.gene07860:CDS | 50.0% | |
| GCCATATACAAAGCCCTAGC+CGG | + | chr8.3:71493233-71493252 | None:intergenic | 50.0% | |
| GCCCACCATACTGCATTTGA+AGG | + | chr8.3:71493563-71493582 | None:intergenic | 50.0% | |
| GGTCCTAGGGGTTTGTATAG+AGG | - | chr8.3:71493509-71493528 | MS.gene07860:CDS | 50.0% | |
| GGTTAAGGAAGGTGGTTGGA+TGG | - | chr8.3:71493865-71493884 | MS.gene07860:CDS | 50.0% | |
| TCAGGGAATCAGTGCAGCAA+TGG | - | chr8.3:71493721-71493740 | MS.gene07860:CDS | 50.0% | |
| TGAGGAAGAGGTAGAGTCAG+AGG | - | chr8.3:71492965-71492984 | MS.gene07860:CDS | 50.0% | |
| TGGACCAACAGTGATGCAGA+CGG | - | chr8.3:71493832-71493851 | MS.gene07860:CDS | 50.0% | |
| TGTGAGAACGGATGGTCCTA+GGG | - | chr8.3:71493496-71493515 | MS.gene07860:CDS | 50.0% | |
| TTGTGAGAACGGATGGTCCT+AGG | - | chr8.3:71493495-71493514 | MS.gene07860:CDS | 50.0% | |
| TTTGTATATGGCGGCGCTTG+AGG | - | chr8.3:71493241-71493260 | MS.gene07860:CDS | 50.0% | |
| ! | GGCTAGGGCTTTGTATATGG+CGG | - | chr8.3:71493232-71493251 | MS.gene07860:CDS | 50.0% |
| !! | AGAGCGTTGTATCGTGGGTT+TGG | - | chr8.3:71493185-71493204 | MS.gene07860:CDS | 50.0% |
| !! | GGGTTTAGAGCGTTGTATCG+TGG | - | chr8.3:71493179-71493198 | MS.gene07860:CDS | 50.0% |
| !!! | CTCGGTGCTGCTTTGTTTTC+TGG | - | chr8.3:71493050-71493069 | MS.gene07860:CDS | 50.0% |
| !!! | GGAACTGCTACGGTTGGTTT+TGG | - | chr8.3:71493281-71493300 | MS.gene07860:CDS | 50.0% |
| AGGGGCTAATGTGTTGAGGC+CGG | - | chr8.3:71493676-71493695 | MS.gene07860:CDS | 55.0% | |
| ATCAGTGCAGCAATGGCAGG+AGG | - | chr8.3:71493728-71493747 | MS.gene07860:CDS | 55.0% | |
| GAGTAACAAGAAAGGCGGGG+AGG | - | chr8.3:71493634-71493653 | MS.gene07860:CDS | 55.0% | |
| GATGGGTACAATCCCGGCTA+GGG | - | chr8.3:71493217-71493236 | MS.gene07860:CDS | 55.0% | |
| GCATCACTGTTGGTCCACGA+CGG | + | chr8.3:71493829-71493848 | None:intergenic | 55.0% | |
| GGAATCAGTGCAGCAATGGC+AGG | - | chr8.3:71493725-71493744 | MS.gene07860:CDS | 55.0% | |
| GTGAGAACGGATGGTCCTAG+GGG | - | chr8.3:71493497-71493516 | MS.gene07860:CDS | 55.0% | |
| ! | AGGATGGTTTGGGGTGGAGT+TGG | - | chr8.3:71493602-71493621 | MS.gene07860:CDS | 55.0% |
| ! | CCCGGCTAGGGCTTTGTATA+TGG | - | chr8.3:71493229-71493248 | MS.gene07860:CDS | 55.0% |
| ! | GCACAAAGGATGGTTTGGGG+TGG | - | chr8.3:71493596-71493615 | MS.gene07860:CDS | 55.0% |
| ! | TGATGGGTACAATCCCGGCT+AGG | - | chr8.3:71493216-71493235 | MS.gene07860:CDS | 55.0% |
| GGCGATGAGAATGGCCGTCG+TGG | - | chr8.3:71493812-71493831 | MS.gene07860:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 71492948 | 71494006 | 71492948 | ID=MS.gene07860 |
| chr8.3 | mRNA | 71492948 | 71494006 | 71492948 | ID=MS.gene07860.t1;Parent=MS.gene07860 |
| chr8.3 | exon | 71492948 | 71494006 | 71492948 | ID=MS.gene07860.t1.exon1;Parent=MS.gene07860.t1 |
| chr8.3 | CDS | 71492948 | 71494006 | 71492948 | ID=cds.MS.gene07860.t1;Parent=MS.gene07860.t1 |
| Gene Sequence |
| Protein sequence |
