Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07906.t1 | XP_003627277.1 | 95.2 | 209 | 10 | 0 | 1 | 209 | 1 | 209 | 2.50E-106 | 394.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07906.t1 | Q9ZRA4 | 58.5 | 207 | 84 | 2 | 3 | 207 | 1 | 207 | 2.1e-61 | 236.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07906.t1 | G7L6U9 | 95.2 | 209 | 10 | 0 | 1 | 209 | 1 | 209 | 1.8e-106 | 394.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene05104 | MS.gene07906 | 0.801255 | 9.82E-49 | -1.69E-46 |
| MS.gene056856 | MS.gene07906 | 0.80292 | 4.46E-49 | -1.69E-46 |
| MS.gene057261 | MS.gene07906 | 0.818483 | 1.91E-52 | -1.69E-46 |
| MS.gene058099 | MS.gene07906 | 0.813148 | 2.96E-51 | -1.69E-46 |
| MS.gene058157 | MS.gene07906 | -0.813003 | 3.19E-51 | -1.69E-46 |
| MS.gene060405 | MS.gene07906 | 0.803872 | 2.83E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07906.t1 | MTR_8g020630 | 95.215 | 209 | 10 | 0 | 1 | 209 | 1 | 209 | 1.53e-144 | 401 |
| MS.gene07906.t1 | MTR_8g020650 | 94.737 | 209 | 11 | 0 | 1 | 209 | 1 | 209 | 1.87e-137 | 383 |
| MS.gene07906.t1 | MTR_8g020670 | 88.517 | 209 | 24 | 0 | 1 | 209 | 1 | 209 | 1.31e-133 | 373 |
| MS.gene07906.t1 | MTR_2g044040 | 73.684 | 209 | 55 | 0 | 1 | 209 | 1 | 209 | 6.58e-111 | 316 |
| MS.gene07906.t1 | MTR_0284s0010 | 73.684 | 209 | 55 | 0 | 1 | 209 | 1 | 209 | 6.77e-109 | 311 |
| MS.gene07906.t1 | MTR_2g045100 | 70.813 | 209 | 61 | 0 | 1 | 209 | 1 | 209 | 1.85e-106 | 305 |
| MS.gene07906.t1 | MTR_2g036120 | 70.531 | 207 | 61 | 0 | 1 | 207 | 1 | 207 | 3.89e-105 | 301 |
| MS.gene07906.t1 | MTR_3g019710 | 70.813 | 209 | 61 | 0 | 1 | 209 | 1 | 209 | 9.23e-105 | 300 |
| MS.gene07906.t1 | MTR_5g031460 | 68.421 | 209 | 66 | 0 | 1 | 209 | 1 | 209 | 1.14e-100 | 290 |
| MS.gene07906.t1 | MTR_3g019650 | 68.900 | 209 | 64 | 1 | 1 | 209 | 1 | 208 | 1.21e-99 | 287 |
| MS.gene07906.t1 | MTR_3g019680 | 68.900 | 209 | 64 | 1 | 1 | 209 | 1 | 208 | 3.10e-99 | 286 |
| MS.gene07906.t1 | MTR_7g011340 | 70.335 | 209 | 62 | 0 | 1 | 209 | 1 | 209 | 3.13e-98 | 284 |
| MS.gene07906.t1 | MTR_4g010340 | 58.937 | 207 | 83 | 2 | 3 | 209 | 1 | 205 | 6.03e-84 | 248 |
| MS.gene07906.t1 | MTR_3g019810 | 64.532 | 203 | 66 | 1 | 1 | 203 | 107 | 303 | 1.26e-83 | 251 |
| MS.gene07906.t1 | MTR_8g020590 | 57.895 | 209 | 85 | 3 | 3 | 209 | 1 | 208 | 5.43e-81 | 240 |
| MS.gene07906.t1 | MTR_2g019780 | 56.744 | 215 | 84 | 3 | 1 | 209 | 1 | 212 | 2.28e-80 | 239 |
| MS.gene07906.t1 | MTR_3g073170 | 56.338 | 213 | 86 | 3 | 1 | 209 | 1 | 210 | 1.31e-79 | 237 |
| MS.gene07906.t1 | MTR_2g041550 | 55.981 | 209 | 88 | 3 | 3 | 209 | 4 | 210 | 4.32e-78 | 233 |
| MS.gene07906.t1 | MTR_8g020760 | 56.398 | 211 | 90 | 2 | 1 | 209 | 1 | 211 | 4.71e-78 | 233 |
| MS.gene07906.t1 | MTR_2g041580 | 54.976 | 211 | 92 | 3 | 1 | 209 | 1 | 210 | 3.80e-77 | 231 |
| MS.gene07906.t1 | MTR_8g020610 | 56.459 | 209 | 87 | 3 | 3 | 209 | 1 | 207 | 2.69e-76 | 228 |
| MS.gene07906.t1 | MTR_2g041570 | 54.502 | 211 | 93 | 3 | 1 | 209 | 1 | 210 | 2.87e-76 | 228 |
| MS.gene07906.t1 | MTR_8g020770 | 54.245 | 212 | 94 | 3 | 1 | 209 | 1 | 212 | 4.49e-76 | 228 |
| MS.gene07906.t1 | MTR_8g020763 | 57.692 | 182 | 76 | 1 | 29 | 209 | 9 | 190 | 6.88e-72 | 216 |
| MS.gene07906.t1 | MTR_8g020750 | 52.830 | 212 | 97 | 3 | 1 | 209 | 1 | 212 | 9.49e-72 | 217 |
| MS.gene07906.t1 | MTR_4g027415 | 72.535 | 142 | 39 | 0 | 1 | 142 | 1 | 142 | 2.74e-64 | 196 |
| MS.gene07906.t1 | MTR_2g072560 | 66.087 | 115 | 39 | 0 | 1 | 115 | 1 | 115 | 4.59e-51 | 162 |
| MS.gene07906.t1 | MTR_3g019610 | 70.755 | 106 | 31 | 0 | 1 | 106 | 1 | 106 | 1.17e-48 | 155 |
| MS.gene07906.t1 | MTR_7g111240 | 37.500 | 192 | 116 | 3 | 21 | 208 | 23 | 214 | 1.45e-36 | 127 |
| MS.gene07906.t1 | MTR_1g112200 | 36.408 | 206 | 122 | 6 | 3 | 205 | 8 | 207 | 7.05e-36 | 125 |
| MS.gene07906.t1 | MTR_7g111240 | 37.500 | 192 | 113 | 4 | 21 | 208 | 23 | 211 | 1.23e-35 | 125 |
| MS.gene07906.t1 | MTR_1g085140 | 36.126 | 191 | 118 | 3 | 22 | 208 | 28 | 218 | 1.60e-35 | 125 |
| MS.gene07906.t1 | MTR_1g085500 | 34.286 | 210 | 131 | 4 | 3 | 208 | 11 | 217 | 1.89e-34 | 122 |
| MS.gene07906.t1 | MTR_2g031270 | 36.649 | 191 | 112 | 4 | 21 | 205 | 26 | 213 | 5.50e-34 | 120 |
| MS.gene07906.t1 | MTR_2g030855 | 35.859 | 198 | 118 | 4 | 14 | 205 | 18 | 212 | 7.72e-34 | 120 |
| MS.gene07906.t1 | MTR_1g060120 | 34.375 | 192 | 118 | 2 | 22 | 205 | 30 | 221 | 1.91e-33 | 119 |
| MS.gene07906.t1 | MTR_1g079490 | 34.597 | 211 | 130 | 3 | 3 | 205 | 7 | 217 | 4.77e-33 | 118 |
| MS.gene07906.t1 | MTR_6g005330 | 37.500 | 216 | 123 | 5 | 1 | 205 | 1 | 215 | 7.51e-33 | 118 |
| MS.gene07906.t1 | MTR_4g017050 | 36.530 | 219 | 120 | 7 | 1 | 204 | 1 | 215 | 1.21e-32 | 117 |
| MS.gene07906.t1 | MTR_5g046410 | 36.530 | 219 | 120 | 7 | 1 | 204 | 1 | 215 | 1.61e-32 | 117 |
| MS.gene07906.t1 | MTR_5g046430 | 36.530 | 219 | 120 | 7 | 1 | 204 | 1 | 215 | 1.61e-32 | 117 |
| MS.gene07906.t1 | MTR_4g017030 | 36.530 | 219 | 120 | 7 | 1 | 204 | 1 | 215 | 1.61e-32 | 117 |
| MS.gene07906.t1 | MTR_6g005310 | 39.683 | 189 | 106 | 4 | 24 | 205 | 28 | 215 | 1.66e-32 | 117 |
| MS.gene07906.t1 | MTR_6g005360 | 39.683 | 189 | 106 | 4 | 24 | 205 | 28 | 215 | 2.69e-32 | 116 |
| MS.gene07906.t1 | MTR_2g019250 | 36.073 | 219 | 121 | 7 | 1 | 204 | 1 | 215 | 4.56e-32 | 116 |
| MS.gene07906.t1 | MTR_4g017040 | 35.484 | 217 | 125 | 6 | 1 | 204 | 1 | 215 | 7.62e-32 | 115 |
| MS.gene07906.t1 | MTR_6g005340 | 39.153 | 189 | 107 | 4 | 24 | 205 | 28 | 215 | 8.91e-32 | 115 |
| MS.gene07906.t1 | MTR_1g023770 | 33.010 | 206 | 126 | 4 | 6 | 205 | 10 | 209 | 1.34e-31 | 114 |
| MS.gene07906.t1 | MTR_6g005380 | 37.500 | 192 | 111 | 4 | 11 | 194 | 15 | 205 | 2.29e-28 | 106 |
| MS.gene07906.t1 | MTR_2g030895 | 35.465 | 172 | 104 | 4 | 41 | 205 | 7 | 178 | 7.28e-28 | 103 |
| MS.gene07906.t1 | MTR_2g030865 | 35.465 | 172 | 104 | 4 | 41 | 205 | 7 | 178 | 2.73e-26 | 100 |
| MS.gene07906.t1 | MTR_8g007300 | 36.567 | 134 | 84 | 1 | 37 | 169 | 47 | 180 | 6.37e-26 | 100 |
| MS.gene07906.t1 | MTR_5g036570 | 31.937 | 191 | 125 | 4 | 22 | 207 | 31 | 221 | 7.47e-26 | 100 |
| MS.gene07906.t1 | MTR_3g096330 | 34.659 | 176 | 104 | 3 | 5 | 169 | 556 | 731 | 1.11e-25 | 104 |
| MS.gene07906.t1 | MTR_6g005350 | 33.962 | 212 | 127 | 5 | 6 | 205 | 4 | 214 | 4.61e-25 | 97.8 |
| MS.gene07906.t1 | MTR_2g086630 | 38.365 | 159 | 92 | 4 | 52 | 205 | 47 | 204 | 1.99e-23 | 93.2 |
| MS.gene07906.t1 | MTR_2g086620 | 37.736 | 159 | 93 | 4 | 52 | 205 | 47 | 204 | 9.30e-23 | 91.7 |
| MS.gene07906.t1 | MTR_2g031300 | 44.340 | 106 | 55 | 3 | 41 | 142 | 7 | 112 | 8.56e-22 | 87.8 |
| MS.gene07906.t1 | MTR_2g086640 | 36.842 | 133 | 82 | 2 | 77 | 207 | 73 | 205 | 1.11e-20 | 85.9 |
| MS.gene07906.t1 | MTR_2g096110 | 35.915 | 142 | 74 | 3 | 40 | 178 | 2 | 129 | 3.65e-19 | 80.5 |
| MS.gene07906.t1 | MTR_4g052770 | 36.296 | 135 | 84 | 2 | 73 | 205 | 71 | 205 | 1.08e-18 | 80.9 |
| MS.gene07906.t1 | MTR_4g052780 | 36.296 | 135 | 84 | 2 | 73 | 205 | 71 | 205 | 1.87e-18 | 80.1 |
| MS.gene07906.t1 | MTR_7g029500 | 52.830 | 53 | 25 | 0 | 150 | 202 | 38 | 90 | 2.09e-13 | 63.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07906.t1 | AT1G72610 | 60.622 | 193 | 74 | 2 | 19 | 209 | 16 | 208 | 1.57e-77 | 231 |
| MS.gene07906.t1 | AT5G20630 | 55.502 | 209 | 91 | 2 | 1 | 207 | 1 | 209 | 3.62e-75 | 226 |
| MS.gene07906.t1 | AT5G39160 | 40.722 | 194 | 105 | 4 | 20 | 206 | 26 | 216 | 1.56e-40 | 137 |
| MS.gene07906.t1 | AT5G39190 | 41.327 | 196 | 105 | 5 | 20 | 206 | 26 | 220 | 3.15e-40 | 137 |
| MS.gene07906.t1 | AT5G39160 | 40.816 | 196 | 106 | 5 | 20 | 206 | 26 | 220 | 1.04e-39 | 135 |
| MS.gene07906.t1 | AT3G05950 | 41.969 | 193 | 102 | 5 | 22 | 205 | 30 | 221 | 1.68e-39 | 135 |
| MS.gene07906.t1 | AT5G39130 | 41.327 | 196 | 105 | 5 | 20 | 206 | 26 | 220 | 1.91e-39 | 135 |
| MS.gene07906.t1 | AT3G04200 | 39.378 | 193 | 109 | 5 | 22 | 206 | 31 | 223 | 5.57e-39 | 134 |
| MS.gene07906.t1 | AT5G26700 | 37.198 | 207 | 126 | 4 | 5 | 208 | 7 | 212 | 9.64e-39 | 133 |
| MS.gene07906.t1 | AT5G39110 | 41.361 | 191 | 103 | 4 | 24 | 205 | 30 | 220 | 1.91e-38 | 132 |
| MS.gene07906.t1 | AT1G09560 | 40.957 | 188 | 107 | 3 | 22 | 205 | 28 | 215 | 3.79e-38 | 131 |
| MS.gene07906.t1 | AT3G62020 | 35.545 | 211 | 126 | 5 | 8 | 208 | 8 | 218 | 6.60e-38 | 131 |
| MS.gene07906.t1 | AT1G18980 | 36.765 | 204 | 123 | 4 | 6 | 205 | 17 | 218 | 2.04e-37 | 130 |
| MS.gene07906.t1 | AT3G05930 | 39.153 | 189 | 110 | 3 | 22 | 205 | 27 | 215 | 2.08e-37 | 129 |
| MS.gene07906.t1 | AT5G39180 | 40.625 | 192 | 103 | 5 | 24 | 205 | 29 | 219 | 7.39e-37 | 128 |
| MS.gene07906.t1 | AT1G02335 | 34.742 | 213 | 131 | 4 | 4 | 208 | 6 | 218 | 9.48e-37 | 128 |
| MS.gene07906.t1 | AT5G39120 | 38.426 | 216 | 121 | 6 | 1 | 205 | 5 | 219 | 1.05e-36 | 128 |
| MS.gene07906.t1 | AT5G39150 | 40.625 | 192 | 103 | 5 | 24 | 205 | 29 | 219 | 2.36e-36 | 127 |
| MS.gene07906.t1 | AT3G62020 | 37.853 | 177 | 105 | 4 | 37 | 208 | 13 | 189 | 1.08e-35 | 124 |
| MS.gene07906.t1 | AT5G39190 | 40.449 | 178 | 98 | 3 | 37 | 206 | 21 | 198 | 1.31e-35 | 124 |
| MS.gene07906.t1 | AT5G39160 | 39.888 | 178 | 99 | 3 | 37 | 206 | 21 | 198 | 4.23e-35 | 123 |
| MS.gene07906.t1 | AT3G04150 | 35.204 | 196 | 119 | 4 | 22 | 209 | 30 | 225 | 1.30e-33 | 120 |
| MS.gene07906.t1 | AT5G38960 | 36.000 | 200 | 111 | 5 | 20 | 206 | 28 | 223 | 2.53e-33 | 119 |
| MS.gene07906.t1 | AT3G10080 | 38.265 | 196 | 103 | 7 | 22 | 205 | 30 | 219 | 2.89e-33 | 119 |
| MS.gene07906.t1 | AT1G18970 | 35.821 | 201 | 123 | 4 | 9 | 205 | 20 | 218 | 7.15e-33 | 118 |
| MS.gene07906.t1 | AT3G04150 | 35.556 | 180 | 109 | 3 | 37 | 209 | 59 | 238 | 1.08e-32 | 118 |
| MS.gene07906.t1 | AT3G10080 | 38.265 | 196 | 103 | 7 | 22 | 205 | 101 | 290 | 1.37e-32 | 119 |
| MS.gene07906.t1 | AT4G14630 | 34.951 | 206 | 117 | 5 | 13 | 205 | 21 | 222 | 1.22e-31 | 115 |
| MS.gene07906.t1 | AT5G38940 | 38.144 | 194 | 112 | 5 | 20 | 205 | 28 | 221 | 2.39e-31 | 114 |
| MS.gene07906.t1 | AT5G38940 | 38.144 | 194 | 112 | 5 | 20 | 205 | 52 | 245 | 3.35e-31 | 114 |
| MS.gene07906.t1 | AT1G74820 | 36.022 | 186 | 113 | 4 | 24 | 205 | 42 | 225 | 6.68e-31 | 113 |
| MS.gene07906.t1 | AT5G61750 | 41.176 | 136 | 78 | 2 | 37 | 170 | 40 | 175 | 1.09e-29 | 109 |
| MS.gene07906.t1 | AT5G38930 | 37.113 | 194 | 114 | 5 | 20 | 205 | 28 | 221 | 1.14e-29 | 110 |
| MS.gene07906.t1 | AT3G04190 | 32.338 | 201 | 128 | 4 | 13 | 205 | 21 | 221 | 1.86e-29 | 109 |
| MS.gene07906.t1 | AT5G38910 | 36.869 | 198 | 109 | 5 | 20 | 205 | 26 | 219 | 2.59e-29 | 108 |
| MS.gene07906.t1 | AT3G04170 | 34.518 | 197 | 112 | 6 | 20 | 205 | 28 | 218 | 5.07e-29 | 108 |
| MS.gene07906.t1 | AT3G04180 | 33.505 | 194 | 121 | 4 | 20 | 205 | 28 | 221 | 1.66e-27 | 104 |
| MS.gene07906.t1 | AT1G10460 | 32.222 | 180 | 93 | 4 | 7 | 183 | 38 | 191 | 2.49e-19 | 82.8 |
| MS.gene07906.t1 | AT5G38910 | 30.256 | 195 | 89 | 5 | 20 | 205 | 26 | 182 | 1.02e-14 | 69.7 |
Find 54 sgRNAs with CRISPR-Local
Find 62 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAAAGAGTTGTTGGTGTTTC+CGG | 0.213827 | 8.3:+70514515 | None:intergenic |
| CAACTCAGAAGGTAAATTAT+TGG | 0.312792 | 8.3:+70514155 | None:intergenic |
| GTTGAAACCGGGCGATCTTA+TGG | 0.323878 | 8.3:-70514295 | MS.gene07906:CDS |
| CGGAGATTCCAAGTCCATTA+AGG | 0.359129 | 8.3:+70514451 | None:intergenic |
| TGATAGCCTGAAGGAGTATC+TGG | 0.361912 | 8.3:+70514594 | None:intergenic |
| GTCTTTCATGGTTTCGTAGC+CGG | 0.393248 | 8.3:-70514534 | MS.gene07906:CDS |
| TTGAACTATTATCAATAATT+CGG | 0.400836 | 8.3:+70514368 | None:intergenic |
| TTTCCAGGCCTTAATGGACT+TGG | 0.418861 | 8.3:-70514459 | MS.gene07906:CDS |
| CGGTTTGGTGGAAGAGGGTA+GGG | 0.424148 | 8.3:-70514081 | None:intergenic |
| TCTTCACTTGTGCAAGATCA+AGG | 0.425262 | 8.3:+70514115 | None:intergenic |
| TTATTCTAAGATGTTGAAAC+CGG | 0.428787 | 8.3:-70514307 | MS.gene07906:CDS |
| AAGGCTCCAGATACTCCTTC+AGG | 0.435016 | 8.3:-70514600 | MS.gene07906:CDS |
| AAAATCATTGACAGAAGCTT+GGG | 0.456575 | 8.3:+70514638 | None:intergenic |
| AAGCTAAAGGCTCGGTTTGG+TGG | 0.464757 | 8.3:-70514093 | MS.gene07906:CDS |
| TGCAGCATTGAAAGAGTTGT+TGG | 0.465463 | 8.3:+70514506 | None:intergenic |
| TAATTTACCTTCTGAGTTGG+TGG | 0.466180 | 8.3:-70514151 | MS.gene07906:CDS |
| CAATAATTTACCTTCTGAGT+TGG | 0.469901 | 8.3:-70514154 | MS.gene07906:CDS |
| TATCAATAATTCGGTAGCAC+CGG | 0.476275 | 8.3:+70514377 | None:intergenic |
| TCGGTTTGGTGGAAGAGGGT+AGG | 0.483402 | 8.3:-70514082 | None:intergenic |
| TTTCAAATTAACACTGGTAA+GGG | 0.484943 | 8.3:-70514249 | MS.gene07906:CDS |
| AAGAAGCTAAAGGCTCGGTT+TGG | 0.499905 | 8.3:-70514096 | MS.gene07906:CDS |
| GAATTATTGATAATAGTTCA+AGG | 0.514232 | 8.3:-70514366 | MS.gene07906:CDS |
| AAGGCTCGGTTTGGTGGAAG+AGG | 0.518444 | 8.3:-70514087 | MS.gene07906:CDS |
| TTGACAGAAGCTTGGGAAGT+GGG | 0.521205 | 8.3:+70514645 | None:intergenic |
| GTTCAAGGTGAAATAACTGC+TGG | 0.522312 | 8.3:-70514351 | MS.gene07906:CDS |
| AGATCAAGTAATTGAGCACC+AGG | 0.524185 | 8.3:+70514189 | None:intergenic |
| ATCAATAATTCGGTAGCACC+GGG | 0.538906 | 8.3:+70514378 | None:intergenic |
| ATTCCAAGTCCATTAAGGCC+TGG | 0.549761 | 8.3:+70514456 | None:intergenic |
| ATTGACAGAAGCTTGGGAAG+TGG | 0.549798 | 8.3:+70514644 | None:intergenic |
| GTCTGATGTGATATTTGCAA+GGG | 0.551461 | 8.3:+70514563 | None:intergenic |
| GCACCGGGATGAGTATGCAT+TGG | 0.553990 | 8.3:+70514393 | None:intergenic |
| GGTTTGGTGGAAGAGGGTAG+GGG | 0.560788 | 8.3:-70514080 | None:intergenic |
| ATTCCAATGCATACTCATCC+CGG | 0.573458 | 8.3:-70514396 | MS.gene07906:CDS |
| CGTCTGATGTGATATTTGCA+AGG | 0.576346 | 8.3:+70514562 | None:intergenic |
| TGCACAAGTGAAGAAGCTAA+AGG | 0.586569 | 8.3:-70514106 | MS.gene07906:CDS |
| TGAAAATGCAACATCCCTTG+TGG | 0.593070 | 8.3:+70514267 | None:intergenic |
| CGCTATGTCTAACCGCGCTG+CGG | 0.604451 | 8.3:+70514431 | None:intergenic |
| GTTTGTGCCACCAACTCAGA+AGG | 0.612692 | 8.3:+70514144 | None:intergenic |
| ATCTTAGAATAAACCGCGGT+AGG | 0.612903 | 8.3:+70514318 | None:intergenic |
| TGACAGAAGCTTGGGAAGTG+GGG | 0.613146 | 8.3:+70514646 | None:intergenic |
| AAGTGAAGAAGCTAAAGGCT+CGG | 0.623995 | 8.3:-70514101 | MS.gene07906:CDS |
| TATTCTAAGATGTTGAAACC+GGG | 0.635109 | 8.3:-70514306 | MS.gene07906:CDS |
| TGGATTTCTGACACCTACCG+CGG | 0.639675 | 8.3:-70514331 | MS.gene07906:CDS |
| TGGAAAATCAGTGACGAATG+CGG | 0.643979 | 8.3:+70514476 | None:intergenic |
| CGGTTAGACATAGCGGAAGG+TGG | 0.644542 | 8.3:-70514423 | MS.gene07906:CDS |
| AGGCTCGGTTTGGTGGAAGA+GGG | 0.645017 | 8.3:-70514086 | MS.gene07906:CDS |
| CGCAGCGCGGTTAGACATAG+CGG | 0.655117 | 8.3:-70514430 | MS.gene07906:CDS |
| GCGCGGTTAGACATAGCGGA+AGG | 0.662984 | 8.3:-70514426 | MS.gene07906:CDS |
| TCTGATGTGATATTTGCAAG+GGG | 0.669912 | 8.3:+70514564 | None:intergenic |
| GGTTTGCAGTGATAGCCTGA+AGG | 0.681067 | 8.3:+70514585 | None:intergenic |
| AAAATCAGTGACGAATGCGG+AGG | 0.711598 | 8.3:+70514479 | None:intergenic |
| CAACATCTTAGAATAAACCG+CGG | 0.729066 | 8.3:+70514314 | None:intergenic |
| GATCAAGTAATTGAGCACCA+GGG | 0.730668 | 8.3:+70514190 | None:intergenic |
| GACTTGGAATCTCCGCAGCG+CGG | 0.753131 | 8.3:-70514443 | MS.gene07906:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTGAACTATTATCAATAATT+CGG | + | chr8.3:70514408-70514427 | None:intergenic | 15.0% |
| !!! | GAATTATTGATAATAGTTCA+AGG | - | chr8.3:70514407-70514426 | MS.gene07906:CDS | 20.0% |
| !!! | TTTTTGTGTAGCAAATTTAA+AGG | - | chr8.3:70514154-70514173 | MS.gene07906:CDS | 20.0% |
| ! | TTTCAAATTAACACTGGTAA+GGG | - | chr8.3:70514524-70514543 | MS.gene07906:CDS | 25.0% |
| !! | TTGCATTTTCAAATTAACAC+TGG | - | chr8.3:70514518-70514537 | MS.gene07906:CDS | 25.0% |
| !! | TTTTCAAATTAACACTGGTA+AGG | - | chr8.3:70514523-70514542 | MS.gene07906:CDS | 25.0% |
| !!! | TTATTCTAAGATGTTGAAAC+CGG | - | chr8.3:70514466-70514485 | MS.gene07906:CDS | 25.0% |
| AAAAATCATTGACAGAAGCT+TGG | + | chr8.3:70514139-70514158 | None:intergenic | 30.0% | |
| AAAATCATTGACAGAAGCTT+GGG | + | chr8.3:70514138-70514157 | None:intergenic | 30.0% | |
| CAACTCAGAAGGTAAATTAT+TGG | + | chr8.3:70514621-70514640 | None:intergenic | 30.0% | |
| CAATAATTTACCTTCTGAGT+TGG | - | chr8.3:70514619-70514638 | MS.gene07906:CDS | 30.0% | |
| ! | ATCTTATGGTTTTTCCACAA+GGG | - | chr8.3:70514492-70514511 | MS.gene07906:CDS | 30.0% |
| !! | TATTCTAAGATGTTGAAACC+GGG | - | chr8.3:70514467-70514486 | MS.gene07906:CDS | 30.0% |
| CAACATCTTAGAATAAACCG+CGG | + | chr8.3:70514462-70514481 | None:intergenic | 35.0% | |
| GTCTGATGTGATATTTGCAA+GGG | + | chr8.3:70514213-70514232 | None:intergenic | 35.0% | |
| TATCAATAATTCGGTAGCAC+CGG | + | chr8.3:70514399-70514418 | None:intergenic | 35.0% | |
| TCTGATGTGATATTTGCAAG+GGG | + | chr8.3:70514212-70514231 | None:intergenic | 35.0% | |
| ! | GATCTTATGGTTTTTCCACA+AGG | - | chr8.3:70514491-70514510 | MS.gene07906:CDS | 35.0% |
| ! | TAATTTACCTTCTGAGTTGG+TGG | - | chr8.3:70514622-70514641 | MS.gene07906:CDS | 35.0% |
| ! | TCAGACGATTTTGTCTTTCA+TGG | - | chr8.3:70514227-70514246 | MS.gene07906:CDS | 35.0% |
| AAGTGAAGAAGCTAAAGGCT+CGG | - | chr8.3:70514672-70514691 | MS.gene07906:CDS | 40.0% | |
| AGATCAAGTAATTGAGCACC+AGG | + | chr8.3:70514587-70514606 | None:intergenic | 40.0% | |
| ATCAATAATTCGGTAGCACC+GGG | + | chr8.3:70514398-70514417 | None:intergenic | 40.0% | |
| ATCTTAGAATAAACCGCGGT+AGG | + | chr8.3:70514458-70514477 | None:intergenic | 40.0% | |
| ATTCCAATGCATACTCATCC+CGG | - | chr8.3:70514377-70514396 | MS.gene07906:CDS | 40.0% | |
| CGTCTGATGTGATATTTGCA+AGG | + | chr8.3:70514214-70514233 | None:intergenic | 40.0% | |
| GATCAAGTAATTGAGCACCA+GGG | + | chr8.3:70514586-70514605 | None:intergenic | 40.0% | |
| GTTCAAGGTGAAATAACTGC+TGG | - | chr8.3:70514422-70514441 | MS.gene07906:CDS | 40.0% | |
| TCTTCACTTGTGCAAGATCA+AGG | + | chr8.3:70514661-70514680 | None:intergenic | 40.0% | |
| TGAAAATGCAACATCCCTTG+TGG | + | chr8.3:70514509-70514528 | None:intergenic | 40.0% | |
| TGCACAAGTGAAGAAGCTAA+AGG | - | chr8.3:70514667-70514686 | MS.gene07906:CDS | 40.0% | |
| TGGAAAATCAGTGACGAATG+CGG | + | chr8.3:70514300-70514319 | None:intergenic | 40.0% | |
| ! | ACTTTTAGTAGTGCTAACCC+TGG | - | chr8.3:70514566-70514585 | MS.gene07906:CDS | 40.0% |
| ! | GAAAGAGTTGTTGGTGTTTC+CGG | + | chr8.3:70514261-70514280 | None:intergenic | 40.0% |
| ! | TGCAGCATTGAAAGAGTTGT+TGG | + | chr8.3:70514270-70514289 | None:intergenic | 40.0% |
| !! | ACTGATTTTCCAGGCCTTAA+TGG | - | chr8.3:70514308-70514327 | MS.gene07906:CDS | 40.0% |
| AAAATCAGTGACGAATGCGG+AGG | + | chr8.3:70514297-70514316 | None:intergenic | 45.0% | |
| ATTCCAAGTCCATTAAGGCC+TGG | + | chr8.3:70514320-70514339 | None:intergenic | 45.0% | |
| ATTGACAGAAGCTTGGGAAG+TGG | + | chr8.3:70514132-70514151 | None:intergenic | 45.0% | |
| CGGAGATTCCAAGTCCATTA+AGG | + | chr8.3:70514325-70514344 | None:intergenic | 45.0% | |
| GGAAAAACCATAAGATCGCC+CGG | + | chr8.3:70514488-70514507 | None:intergenic | 45.0% | |
| GTCTTTCATGGTTTCGTAGC+CGG | - | chr8.3:70514239-70514258 | MS.gene07906:CDS | 45.0% | |
| TTGACAGAAGCTTGGGAAGT+GGG | + | chr8.3:70514131-70514150 | None:intergenic | 45.0% | |
| ! | AAGAAGCTAAAGGCTCGGTT+TGG | - | chr8.3:70514677-70514696 | MS.gene07906:CDS | 45.0% |
| !! | TGATAGCCTGAAGGAGTATC+TGG | + | chr8.3:70514182-70514201 | None:intergenic | 45.0% |
| !! | TTTCCAGGCCTTAATGGACT+TGG | - | chr8.3:70514314-70514333 | MS.gene07906:CDS | 45.0% |
| !!! | GCATTCGTCACTGATTTTCC+AGG | - | chr8.3:70514299-70514318 | MS.gene07906:CDS | 45.0% |
| AAGGCTCCAGATACTCCTTC+AGG | - | chr8.3:70514173-70514192 | MS.gene07906:CDS | 50.0% | |
| GGTTTGCAGTGATAGCCTGA+AGG | + | chr8.3:70514191-70514210 | None:intergenic | 50.0% | |
| GTTGAAACCGGGCGATCTTA+TGG | - | chr8.3:70514478-70514497 | MS.gene07906:CDS | 50.0% | |
| GTTTGTGCCACCAACTCAGA+AGG | + | chr8.3:70514632-70514651 | None:intergenic | 50.0% | |
| TGACAGAAGCTTGGGAAGTG+GGG | + | chr8.3:70514130-70514149 | None:intergenic | 50.0% | |
| TGGATTTCTGACACCTACCG+CGG | - | chr8.3:70514442-70514461 | MS.gene07906:CDS | 50.0% | |
| !! | AAGCTAAAGGCTCGGTTTGG+TGG | - | chr8.3:70514680-70514699 | MS.gene07906:CDS | 50.0% |
| GCACCGGGATGAGTATGCAT+TGG | + | chr8.3:70514383-70514402 | None:intergenic | 55.0% | |
| ! | CGGTTAGACATAGCGGAAGG+TGG | - | chr8.3:70514350-70514369 | MS.gene07906:CDS | 55.0% |
| !! | AAGGCTCGGTTTGGTGGAAG+AGG | - | chr8.3:70514686-70514705 | MS.gene07906:CDS | 55.0% |
| !! | AGGCTCGGTTTGGTGGAAGA+GGG | - | chr8.3:70514687-70514706 | MS.gene07906:CDS | 55.0% |
| CGCAGCGCGGTTAGACATAG+CGG | - | chr8.3:70514343-70514362 | MS.gene07906:CDS | 60.0% | |
| CGCTATGTCTAACCGCGCTG+CGG | + | chr8.3:70514345-70514364 | None:intergenic | 60.0% | |
| GACTTGGAATCTCCGCAGCG+CGG | - | chr8.3:70514330-70514349 | MS.gene07906:CDS | 60.0% | |
| ! | GCGCGGTTAGACATAGCGGA+AGG | - | chr8.3:70514347-70514366 | MS.gene07906:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 70514083 | 70514712 | 70514083 | ID=MS.gene07906 |
| chr8.3 | mRNA | 70514083 | 70514712 | 70514083 | ID=MS.gene07906.t1;Parent=MS.gene07906 |
| chr8.3 | exon | 70514083 | 70514712 | 70514083 | ID=MS.gene07906.t1.exon1;Parent=MS.gene07906.t1 |
| chr8.3 | CDS | 70514083 | 70514712 | 70514083 | ID=cds.MS.gene07906.t1;Parent=MS.gene07906.t1 |
| Gene Sequence |
| Protein sequence |
