Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08145.t1 | ABD33256.2 | 80 | 140 | 16 | 2 | 1 | 128 | 12 | 151 | 5.20E-44 | 187.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08145.t1 | G7L1J1 | 80.0 | 140 | 16 | 2 | 1 | 128 | 1 | 140 | 3.7e-44 | 187.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene04991 | MS.gene08145 | -0.802982 | 4.33E-49 | -1.69E-46 |
| MS.gene050317 | MS.gene08145 | 0.801883 | 7.30E-49 | -1.69E-46 |
| MS.gene06010 | MS.gene08145 | 0.801694 | 7.98E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08145.t1 | MTR_7g086580 | 78.146 | 151 | 16 | 3 | 1 | 134 | 1 | 151 | 1.80e-67 | 202 |
| MS.gene08145.t1 | MTR_7g086730 | 56.322 | 87 | 33 | 2 | 42 | 127 | 385 | 467 | 2.17e-12 | 63.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 18 sgRNAs with CRISPR-Local
Find 52 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGCAATGCAAGAAGATAATA+TGG | 0.244685 | 7.1:-18831060 | MS.gene08145:CDS |
| ATCATGCTACAAGGATGAAT+TGG | 0.329507 | 7.1:-18831443 | MS.gene08145:CDS |
| AGAAGATAATATGGGAAAAT+CGG | 0.365645 | 7.1:-18831051 | MS.gene08145:CDS |
| TGAATCTGCTGTTGCAACTT+TGG | 0.378209 | 7.1:-18830702 | MS.gene08145:CDS |
| TCTTGGATGTATAGGTCAAC+TGG | 0.394861 | 7.1:-18830388 | MS.gene08145:intron |
| TAAAATGCTACTTAGGTTGA+TGG | 0.413008 | 7.1:-18831106 | MS.gene08145:intron |
| AGCAGTTGCAGCAGCTAAGA+AGG | 0.468010 | 7.1:-18830789 | MS.gene08145:CDS |
| TCATGCTACAAGGATGAATT+GGG | 0.473692 | 7.1:-18831442 | MS.gene08145:CDS |
| CTCTGATATCGACGACGAAG+AGG | 0.535143 | 7.1:-18831413 | MS.gene08145:intron |
| TTGTATGTACCTCATCCTCT+TGG | 0.538819 | 7.1:+18831026 | None:intergenic |
| GCATCTCTTTCTCGTTGCAA+AGG | 0.540391 | 7.1:+18831080 | None:intergenic |
| AAAATGCTACTTAGGTTGAT+GGG | 0.556262 | 7.1:-18831105 | MS.gene08145:intron |
| GCAATGCAAGAAGATAATAT+GGG | 0.581162 | 7.1:-18831059 | MS.gene08145:CDS |
| TTTGAATAAATGTACTATGG+AGG | 0.588379 | 7.1:-18830750 | MS.gene08145:CDS |
| AAATCGGAACCAAGAGGATG+AGG | 0.600852 | 7.1:-18831035 | MS.gene08145:intron |
| AACTTTGGCAAAATTCAGAA+CGG | 0.631746 | 7.1:-18830687 | MS.gene08145:intron |
| ATGGGAAAATCGGAACCAAG+AGG | 0.689281 | 7.1:-18831041 | MS.gene08145:CDS |
| ACTGTGTGAATCATGCTACA+AGG | 0.702948 | 7.1:-18831452 | MS.gene08145:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAATCATATTCTAATTTAA+TGG | + | chr7.1:18831390-18831409 | None:intergenic | 10.0% |
| !!! | ATTTATTATTAGTATTTTGT+TGG | - | chr7.1:18831044-18831063 | MS.gene08145:CDS | 10.0% |
| !!! | TCATTTATATTAGTTTTAAA+TGG | - | chr7.1:18831498-18831517 | MS.gene08145:CDS | 10.0% |
| !! | TTCAAAATAAATCTGAAAAT+AGG | + | chr7.1:18830600-18830619 | None:intergenic | 15.0% |
| !!! | TATTTTGAATAAATGTACTA+TGG | - | chr7.1:18831164-18831183 | MS.gene08145:intron | 15.0% |
| !!! | TTACATTTTTAACACAATTT+AGG | + | chr7.1:18831331-18831350 | None:intergenic | 15.0% |
| !!! | TGATTTTTCAAATTAAGCTA+AGG | - | chr7.1:18831403-18831422 | MS.gene08145:intron | 20.0% |
| !!! | TTTCAGATTTATTTTGAACA+TGG | - | chr7.1:18830602-18830621 | MS.gene08145:intron | 20.0% |
| ! | AATAATAAATAGCAAACCAG+TGG | + | chr7.1:18831034-18831053 | None:intergenic | 25.0% |
| ! | AGAAGATAATATGGGAAAAT+CGG | - | chr7.1:18830866-18830885 | MS.gene08145:intron | 25.0% |
| ! | ATTGTTCAAAAACTTGAACT+GGG | - | chr7.1:18830985-18831004 | MS.gene08145:intron | 25.0% |
| ! | ATTTATTGTCATAGTAGTGT+TGG | - | chr7.1:18830925-18830944 | MS.gene08145:intron | 25.0% |
| ! | CTCAACTTTATATTTCTTTC+AGG | - | chr7.1:18831089-18831108 | MS.gene08145:CDS | 25.0% |
| ! | TTTGAATAAATGTACTATGG+AGG | - | chr7.1:18831167-18831186 | MS.gene08145:intron | 25.0% |
| !! | ACTCTAATCTATGTTTTCTA+AGG | - | chr7.1:18831361-18831380 | MS.gene08145:intron | 25.0% |
| !! | ATTTTGAACATGGTCTTTTT+GGG | - | chr7.1:18830612-18830631 | MS.gene08145:intron | 25.0% |
| !!! | AAAGTCAACTATTTTGATCT+TGG | - | chr7.1:18830442-18830461 | MS.gene08145:intron | 25.0% |
| !!! | ATGACTGTTACATTGTTTTT+TGG | - | chr7.1:18830570-18830589 | MS.gene08145:intron | 25.0% |
| !!! | ATTCATTGTTTTGCAATTCA+TGG | + | chr7.1:18830375-18830394 | None:intergenic | 25.0% |
| !!! | TATTTTGAACATGGTCTTTT+TGG | - | chr7.1:18830611-18830630 | MS.gene08145:intron | 25.0% |
| !!! | TTAATTTGCCAGATATATAG+AGG | + | chr7.1:18831449-18831468 | None:intergenic | 25.0% |
| AAACAGAAGTAAATTTGGAG+AGG | + | chr7.1:18830668-18830687 | None:intergenic | 30.0% | |
| AACTTTGGCAAAATTCAGAA+CGG | - | chr7.1:18831230-18831249 | MS.gene08145:intron | 30.0% | |
| GATTGTTCAAAAACTTGAAC+TGG | - | chr7.1:18830984-18831003 | MS.gene08145:intron | 30.0% | |
| GCAACAAACAGAAGTAAATT+TGG | + | chr7.1:18830673-18830692 | None:intergenic | 30.0% | |
| GCAATGCAAGAAGATAATAT+GGG | - | chr7.1:18830858-18830877 | MS.gene08145:intron | 30.0% | |
| TGAACAATCATGATAGACTA+TGG | + | chr7.1:18830973-18830992 | None:intergenic | 30.0% | |
| TGCAATGCAAGAAGATAATA+TGG | - | chr7.1:18830857-18830876 | MS.gene08145:intron | 30.0% | |
| TTTAAATGGAACACCATTCT+TGG | - | chr7.1:18831512-18831531 | MS.gene08145:CDS | 30.0% | |
| ! | TTGCTGTTAAAATGCTACTT+AGG | - | chr7.1:18830804-18830823 | MS.gene08145:CDS | 30.0% |
| !! | AAAATGCTACTTAGGTTGAT+GGG | - | chr7.1:18830812-18830831 | MS.gene08145:intron | 30.0% |
| !! | TAAAATGCTACTTAGGTTGA+TGG | - | chr7.1:18830811-18830830 | MS.gene08145:intron | 30.0% |
| AACACCATTCTTGGATGTAT+AGG | - | chr7.1:18831521-18831540 | MS.gene08145:CDS | 35.0% | |
| ACAATGTAACAGTCATTTGC+AGG | + | chr7.1:18830566-18830585 | None:intergenic | 35.0% | |
| ATCATGCTACAAGGATGAAT+TGG | - | chr7.1:18830474-18830493 | MS.gene08145:intron | 35.0% | |
| CCAGATATATAGAGGAATGA+TGG | + | chr7.1:18831441-18831460 | None:intergenic | 35.0% | |
| CCATCATTCCTCTATATATC+TGG | - | chr7.1:18831438-18831457 | MS.gene08145:CDS | 35.0% | |
| TCATGCTACAAGGATGAATT+GGG | - | chr7.1:18830475-18830494 | MS.gene08145:intron | 35.0% | |
| TTAAGCTAAGGAGTCAAGAA+AGG | - | chr7.1:18831415-18831434 | MS.gene08145:CDS | 35.0% | |
| TTGACCTATACATCCAAGAA+TGG | + | chr7.1:18831528-18831547 | None:intergenic | 35.0% | |
| ! | AGCATACTTATGTTTTCCAC+TGG | - | chr7.1:18831015-18831034 | MS.gene08145:intron | 35.0% |
| ACTGTGTGAATCATGCTACA+AGG | - | chr7.1:18830465-18830484 | MS.gene08145:intron | 40.0% | |
| TCTTGGATGTATAGGTCAAC+TGG | - | chr7.1:18831529-18831548 | MS.gene08145:CDS | 40.0% | |
| TGAATCTGCTGTTGCAACTT+TGG | - | chr7.1:18831215-18831234 | MS.gene08145:intron | 40.0% | |
| TTGGCAAAATTCAGAACGGT+AGG | - | chr7.1:18831234-18831253 | MS.gene08145:intron | 40.0% | |
| TTGTATGTACCTCATCCTCT+TGG | + | chr7.1:18830894-18830913 | None:intergenic | 40.0% | |
| AAATCGGAACCAAGAGGATG+AGG | - | chr7.1:18830882-18830901 | MS.gene08145:intron | 45.0% | |
| AGTCAGTGTGTTGTGCATGA+CGG | + | chr7.1:18830780-18830799 | None:intergenic | 45.0% | |
| ATGGGAAAATCGGAACCAAG+AGG | - | chr7.1:18830876-18830895 | MS.gene08145:intron | 45.0% | |
| GCATCTCTTTCTCGTTGCAA+AGG | + | chr7.1:18830840-18830859 | None:intergenic | 45.0% | |
| AGCAGTTGCAGCAGCTAAGA+AGG | - | chr7.1:18831128-18831147 | MS.gene08145:intron | 50.0% | |
| CTCTGATATCGACGACGAAG+AGG | - | chr7.1:18830504-18830523 | MS.gene08145:intron | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.1 | gene | 18830361 | 18831578 | 18830361 | ID=MS.gene08145 |
| chr7.1 | mRNA | 18830361 | 18831578 | 18830361 | ID=MS.gene08145.t1;Parent=MS.gene08145 |
| chr7.1 | exon | 18831414 | 18831578 | 18831414 | ID=MS.gene08145.t1.exon1;Parent=MS.gene08145.t1 |
| chr7.1 | CDS | 18831414 | 18831578 | 18831414 | ID=cds.MS.gene08145.t1;Parent=MS.gene08145.t1 |
| chr7.1 | exon | 18831036 | 18831113 | 18831036 | ID=MS.gene08145.t1.exon2;Parent=MS.gene08145.t1 |
| chr7.1 | CDS | 18831036 | 18831113 | 18831036 | ID=cds.MS.gene08145.t1;Parent=MS.gene08145.t1 |
| chr7.1 | exon | 18830688 | 18830828 | 18830688 | ID=MS.gene08145.t1.exon3;Parent=MS.gene08145.t1 |
| chr7.1 | CDS | 18830688 | 18830828 | 18830688 | ID=cds.MS.gene08145.t1;Parent=MS.gene08145.t1 |
| chr7.1 | exon | 18830361 | 18830396 | 18830361 | ID=MS.gene08145.t1.exon4;Parent=MS.gene08145.t1 |
| chr7.1 | CDS | 18830361 | 18830396 | 18830361 | ID=cds.MS.gene08145.t1;Parent=MS.gene08145.t1 |
| Gene Sequence |
| Protein sequence |
