AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene08189

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene08189.t1 MTR_7g086000 93.978 465 27 1 1 464 1 465 0.0 865
MS.gene08189.t1 MTR_7g085990 44.864 477 235 12 4 464 2 466 1.11e-132 391
MS.gene08189.t1 MTR_7g085930 44.025 477 239 11 4 464 6 470 7.56e-126 374
MS.gene08189.t1 MTR_7g085960 72.368 228 33 2 237 464 1 198 3.47e-114 334
MS.gene08189.t1 MTR_4g127650 38.745 462 259 13 4 456 10 456 4.20e-95 295
MS.gene08189.t1 MTR_3g049150 36.325 468 278 12 4 464 2 456 2.13e-93 290
MS.gene08189.t1 MTR_3g026950 37.259 467 262 14 4 456 2 451 5.24e-87 273
MS.gene08189.t1 MTR_4g013050 36.797 462 234 12 1 456 1 410 1.13e-86 271
MS.gene08189.t1 MTR_4g097090 37.885 454 263 12 10 456 15 456 2.42e-86 272
MS.gene08189.t1 MTR_4g007540 33.700 454 274 13 4 454 3 432 4.17e-80 255
MS.gene08189.t1 MTR_2g105330 33.040 454 276 13 4 454 3 431 1.11e-79 254
MS.gene08189.t1 MTR_2g064495 32.599 454 278 13 4 454 3 431 6.36e-78 249
MS.gene08189.t1 MTR_8g075610 34.579 428 248 14 31 454 20 419 1.94e-76 245
MS.gene08189.t1 MTR_3g098980 33.171 410 227 18 12 412 22 393 1.82e-53 185
MS.gene08189.t1 MTR_8g096530 31.818 418 243 14 2 412 5 387 3.70e-52 182
MS.gene08189.t1 MTR_7g027175 32.178 404 232 15 11 407 23 391 1.16e-49 175
MS.gene08189.t1 MTR_7g085970 60.811 148 28 4 43 189 8 126 3.63e-47 159
MS.gene08189.t1 MTR_3g049140 36.122 263 149 12 210 464 2 253 1.18e-39 144
MS.gene08189.t1 MTR_7g084940 27.700 426 267 15 2 420 14 405 3.37e-39 147
MS.gene08189.t1 MTR_1g040175 28.993 407 241 16 11 406 16 385 6.51e-39 147
MS.gene08189.t1 MTR_1g040215 27.407 405 250 14 12 407 17 386 7.70e-39 146
MS.gene08189.t1 MTR_7g084910 28.868 433 239 18 2 420 15 392 5.51e-38 144
MS.gene08189.t1 MTR_1g053315 27.959 490 282 20 2 460 16 465 4.78e-36 139
MS.gene08189.t1 MTR_5g090810 28.158 380 218 15 54 407 61 411 1.01e-32 129
MS.gene08189.t1 MTR_5g076700 25.951 447 277 17 11 440 17 426 2.08e-32 129
MS.gene08189.t1 MTR_1g040195 27.387 398 231 19 73 454 55 410 2.59e-32 128
MS.gene08189.t1 MTR_7g114490 26.840 462 272 19 11 451 17 433 2.87e-32 129
MS.gene08189.t1 MTR_3g101930 29.177 401 232 17 44 424 54 422 3.37e-32 128
MS.gene08189.t1 MTR_5g090830 28.421 380 217 15 54 407 61 411 4.33e-32 128
MS.gene08189.t1 MTR_1g081660 24.816 407 272 12 5 406 17 394 1.38e-31 126
MS.gene08189.t1 MTR_7g056087 24.283 453 298 14 1 440 1 421 1.62e-31 125
MS.gene08189.t1 MTR_7g114470 25.221 452 275 18 11 440 18 428 4.59e-31 125
MS.gene08189.t1 MTR_3g071320 27.989 368 247 10 3 362 4 361 2.32e-30 122
MS.gene08189.t1 MTR_2g015430 27.378 431 244 16 57 463 56 441 6.86e-30 121
MS.gene08189.t1 MTR_3g086020 25.586 469 280 18 2 460 3 412 8.31e-30 120
MS.gene08189.t1 MTR_4g055170 26.714 423 254 16 11 412 19 406 1.16e-29 121
MS.gene08189.t1 MTR_1g040315 27.253 466 244 22 12 464 17 400 1.27e-29 120
MS.gene08189.t1 MTR_7g114550 25.466 483 288 22 1 459 7 441 1.30e-29 120
MS.gene08189.t1 MTR_7g114520 25.054 467 285 20 11 452 18 444 3.03e-29 119
MS.gene08189.t1 MTR_3g465990 26.279 430 270 17 4 416 10 409 5.14e-29 119
MS.gene08189.t1 MTR_3g112180 28.700 446 254 18 12 438 20 420 6.53e-29 119
MS.gene08189.t1 MTR_3g101900 28.645 391 220 17 62 431 69 421 1.15e-28 118
MS.gene08189.t1 MTR_1g040320 25.495 404 223 12 11 406 16 349 1.24e-28 117
MS.gene08189.t1 MTR_5g090880 26.209 393 238 15 42 407 46 413 1.40e-28 118
MS.gene08189.t1 MTR_7g114560 25.161 465 292 18 11 459 17 441 3.45e-28 116
MS.gene08189.t1 MTR_3g465980 27.209 430 266 18 4 416 10 409 3.77e-28 116
MS.gene08189.t1 MTR_8g079980 26.256 438 274 17 6 433 6 404 5.53e-28 115
MS.gene08189.t1 MTR_3g465930 29.134 381 233 15 2 363 8 370 3.29e-27 114
MS.gene08189.t1 MTR_7g014160 25.806 496 296 22 3 456 8 473 3.60e-27 114
MS.gene08189.t1 MTR_3g070170 27.352 457 248 20 12 424 20 436 4.41e-27 113
MS.gene08189.t1 MTR_7g014200 23.770 488 311 17 3 456 8 468 4.94e-27 113
MS.gene08189.t1 MTR_7g014250 25.470 479 305 14 5 456 3 456 5.99e-27 113
MS.gene08189.t1 MTR_2g007880 23.256 473 309 14 1 459 14 446 8.69e-27 112
MS.gene08189.t1 MTR_6g053810 27.937 383 236 16 3 365 8 370 9.38e-27 112
MS.gene08189.t1 MTR_7g014330 26.216 473 294 15 9 456 14 456 7.25e-26 110
MS.gene08189.t1 MTR_3g450710 26.190 378 216 16 59 406 65 409 2.00e-25 108
MS.gene08189.t1 MTR_1g094790 25.000 424 266 13 2 407 1 390 2.27e-25 108
MS.gene08189.t1 MTR_3g102020 27.020 396 237 19 62 440 72 432 3.12e-25 108
MS.gene08189.t1 MTR_3g101960 27.407 405 242 19 62 449 71 440 7.96e-25 107
MS.gene08189.t1 MTR_3g450690 26.542 373 218 15 59 406 72 413 1.25e-24 106
MS.gene08189.t1 MTR_0190s0020 27.873 409 238 18 59 449 63 432 1.58e-24 105
MS.gene08189.t1 MTR_3g465910 28.496 379 235 17 2 363 8 367 4.81e-24 105
MS.gene08189.t1 MTR_5g026320 26.196 439 286 15 19 449 35 443 5.67e-24 104
MS.gene08189.t1 MTR_7g014610 25.410 488 311 19 3 456 7 475 8.96e-24 103
MS.gene08189.t1 MTR_3g101970 27.009 448 254 20 19 440 18 418 1.46e-23 103
MS.gene08189.t1 MTR_7g110610 25.877 456 241 21 11 453 12 383 2.13e-23 102
MS.gene08189.t1 MTR_7g014230 25.051 487 311 14 1 459 1 461 2.67e-23 102
MS.gene08189.t1 MTR_8g041770 27.251 411 260 16 47 448 50 430 2.70e-23 102
MS.gene08189.t1 MTR_6g045553 24.946 465 264 17 2 420 12 437 3.07e-23 102
MS.gene08189.t1 MTR_5g084870 22.414 464 301 15 2 452 1 418 7.30e-23 100
MS.gene08189.t1 MTR_3g102010 26.750 400 237 19 62 440 68 432 7.59e-23 101
MS.gene08189.t1 MTR_3g465950 28.354 328 203 13 2 313 8 319 1.19e-22 100
MS.gene08189.t1 MTR_8g079970 24.009 454 292 17 2 442 1 414 3.36e-22 99.0
MS.gene08189.t1 MTR_7g014620 26.374 455 291 18 17 456 7 432 5.90e-22 98.2
MS.gene08189.t1 MTR_8g467580 25.236 424 258 15 4 407 1 385 5.93e-22 98.6
MS.gene08189.t1 MTR_4g094842 26.030 461 281 19 9 448 16 437 5.95e-22 98.6
MS.gene08189.t1 MTR_2g045550 37.241 145 80 2 1 145 1 134 7.32e-22 92.0
MS.gene08189.t1 MTR_8g467590 24.078 461 284 18 11 449 10 426 9.98e-22 97.8
MS.gene08189.t1 MTR_5g081960 24.025 487 262 17 12 431 14 459 1.00e-20 95.1
MS.gene08189.t1 MTR_7g013850 24.235 392 227 15 59 407 68 432 2.43e-20 94.0
MS.gene08189.t1 MTR_6g015830 23.868 486 293 19 10 459 19 463 2.51e-20 93.6
MS.gene08189.t1 MTR_3g465870 28.239 301 194 9 2 292 10 298 3.00e-20 93.2
MS.gene08189.t1 MTR_0502s0010 29.771 262 144 12 98 344 2 238 3.78e-20 89.7
MS.gene08189.t1 MTR_3g088420 27.072 362 225 15 62 413 58 390 7.19e-20 92.0
MS.gene08189.t1 MTR_6g015850 23.941 472 285 19 21 459 30 460 7.28e-20 92.4
MS.gene08189.t1 MTR_7g026860 27.322 366 212 16 62 407 58 389 1.58e-19 90.9
MS.gene08189.t1 MTR_6g015815 25.105 474 280 20 21 459 30 463 2.54e-19 90.5
MS.gene08189.t1 MTR_4g094835 25.971 412 247 20 57 448 62 435 4.88e-19 89.7
MS.gene08189.t1 MTR_4g022825 26.650 409 247 19 59 448 55 429 1.57e-18 88.2
MS.gene08189.t1 MTR_5g084850 23.567 471 306 16 2 464 1 425 1.87e-18 87.8
MS.gene08189.t1 MTR_8g079710 26.161 409 234 15 59 440 59 426 2.61e-18 87.4
MS.gene08189.t1 MTR_6g015315 20.358 447 298 13 39 459 41 455 1.50e-17 85.1
MS.gene08189.t1 MTR_3g028210 26.168 428 268 16 6 407 11 416 5.74e-17 83.6
MS.gene08189.t1 MTR_3g026310 24.638 414 257 16 59 448 55 437 6.17e-17 83.2
MS.gene08189.t1 MTR_1g035890 26.198 313 194 11 9 308 14 302 6.99e-17 83.2
MS.gene08189.t1 MTR_4g022720 25.126 398 256 17 62 448 58 424 1.05e-16 82.4
MS.gene08189.t1 MTR_4g022290 23.944 426 254 16 59 458 60 441 1.33e-16 82.0
MS.gene08189.t1 MTR_2g089650 25.114 438 267 20 3 407 8 417 2.49e-16 81.6
MS.gene08189.t1 MTR_4g022670 24.877 406 245 19 40 416 32 406 2.96e-16 81.3
MS.gene08189.t1 MTR_4g007460 34.722 144 78 5 74 215 2 131 7.41e-16 75.1
MS.gene08189.t1 MTR_2g089765 23.434 495 311 16 7 461 21 487 1.90e-15 79.0
MS.gene08189.t1 MTR_4g022800 25.266 376 223 17 62 413 58 399 2.77e-15 78.2
MS.gene08189.t1 MTR_2g089755 27.143 420 233 23 31 407 40 429 7.88e-15 77.0
MS.gene08189.t1 MTR_4g094838 24.942 429 232 18 49 448 54 421 8.26e-15 76.6
MS.gene08189.t1 MTR_4g022760 26.230 305 197 13 62 352 58 348 1.04e-14 76.3
MS.gene08189.t1 MTR_3g100150 25.237 317 199 10 5 308 12 303 5.55e-14 74.3
MS.gene08189.t1 MTR_2g045540 39.048 105 50 4 210 313 2 93 1.01e-13 67.8
MS.gene08189.t1 MTR_0042s0190 25.249 301 180 14 162 450 3 270 1.59e-13 71.6
MS.gene08189.t1 MTR_2g036780 26.592 267 161 8 24 280 34 275 2.62e-13 72.0
MS.gene08189.t1 MTR_7g014360 23.399 406 252 11 69 456 22 386 4.20e-13 71.2
MS.gene08189.t1 MTR_7g014630 27.895 190 111 6 3 176 8 187 1.99e-12 68.9
MS.gene08189.t1 MTR_2g089670 24.615 390 248 14 40 407 64 429 4.50e-12 68.2
MS.gene08189.t1 MTR_0042s0190 27.551 196 117 8 262 450 28 205 3.00e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene08189.t1 AT2G19070 37.149 463 272 11 1 456 1 451 3.04e-100 308
MS.gene08189.t1 AT5G48930 32.251 462 274 13 2 454 1 432 1.87e-73 238
MS.gene08189.t1 AT5G57840 31.522 460 284 13 2 454 1 436 4.24e-63 211
MS.gene08189.t1 AT5G41040 31.010 416 243 16 2 407 13 394 3.38e-54 187
MS.gene08189.t1 AT5G41040 31.010 416 243 16 2 407 29 410 3.52e-54 188
MS.gene08189.t1 AT3G48720 32.518 409 224 20 11 407 16 384 9.87e-48 170
MS.gene08189.t1 AT5G63560 30.958 407 229 16 11 407 16 380 3.26e-44 160
MS.gene08189.t1 AT1G65450 26.652 469 295 15 2 456 16 449 2.75e-39 147
MS.gene08189.t1 AT3G03480 26.042 480 296 18 2 463 15 453 7.59e-36 138
MS.gene08189.t1 AT5G17540 25.261 479 299 22 3 464 8 444 1.70e-35 137
MS.gene08189.t1 AT3G26040 27.609 460 288 16 11 464 10 430 2.13e-35 137
MS.gene08189.t1 AT1G24430 28.440 436 269 15 2 433 3 399 6.89e-34 132
MS.gene08189.t1 AT2G40230 28.744 414 253 13 4 407 5 386 7.53e-34 132
MS.gene08189.t1 AT1G03390 27.426 474 288 20 2 452 15 455 5.58e-33 130
MS.gene08189.t1 AT1G27620 28.667 450 272 18 11 453 16 423 6.92e-33 130
MS.gene08189.t1 AT3G29635 25.536 466 285 16 22 459 26 457 9.67e-33 129
MS.gene08189.t1 AT1G78990 27.978 361 216 14 59 407 67 395 4.30e-32 127
MS.gene08189.t1 AT5G07080 27.805 410 265 16 11 412 21 407 6.58e-32 127
MS.gene08189.t1 AT1G32910 27.123 365 233 13 52 407 58 398 8.33e-32 127
MS.gene08189.t1 AT3G62160 25.714 420 240 17 11 412 12 377 1.53e-30 123
MS.gene08189.t1 AT3G30280 26.258 457 278 14 11 450 13 427 2.43e-30 122
MS.gene08189.t1 AT4G15390 26.782 463 269 18 11 450 15 430 2.83e-29 119
MS.gene08189.t1 AT4G15390 26.782 463 269 18 11 450 15 430 2.83e-29 119
MS.gene08189.t1 AT2G25150 27.945 433 273 17 11 432 20 424 1.37e-28 118
MS.gene08189.t1 AT3G29670 24.823 423 274 15 44 452 53 445 4.61e-28 116
MS.gene08189.t1 AT3G47170 28.093 388 237 16 11 372 19 390 1.58e-26 112
MS.gene08189.t1 AT2G23510 26.877 413 261 16 11 412 20 402 1.06e-25 109
MS.gene08189.t1 AT5G47950 26.077 441 262 17 11 440 13 400 2.29e-25 108
MS.gene08189.t1 AT5G16410 24.317 366 226 13 57 407 89 418 4.12e-25 108
MS.gene08189.t1 AT5G23940 27.073 410 250 17 59 450 65 443 5.18e-25 107
MS.gene08189.t1 AT1G24420 24.580 476 296 15 3 464 4 430 6.63e-25 107
MS.gene08189.t1 AT1G24420 24.580 476 296 15 3 464 4 430 6.63e-25 107
MS.gene08189.t1 AT3G29590 26.265 415 250 16 66 457 68 449 4.45e-24 104
MS.gene08189.t1 AT1G31490 24.146 410 279 13 1 407 3 383 7.09e-24 103
MS.gene08189.t1 AT5G07870 27.885 416 264 17 59 462 72 463 1.07e-23 103
MS.gene08189.t1 AT3G29680 24.268 478 305 16 4 458 6 449 1.40e-23 103
MS.gene08189.t1 AT3G50280 26.893 383 217 20 59 417 59 402 3.28e-23 102
MS.gene08189.t1 AT5G23970 23.937 447 287 18 2 440 1 402 3.44e-23 102
MS.gene08189.t1 AT5G07860 27.792 403 244 17 59 449 72 439 3.82e-23 102
MS.gene08189.t1 AT4G31910 24.734 376 238 12 59 412 64 416 5.88e-23 101
MS.gene08189.t1 AT1G65450 24.762 315 197 9 152 456 1 285 1.18e-22 98.2
MS.gene08189.t1 AT3G50270 27.208 419 242 22 21 417 30 407 1.50e-22 100
MS.gene08189.t1 AT5G67150 27.610 431 246 25 42 448 47 435 2.22e-21 96.7
MS.gene08189.t1 AT5G39090 25.577 477 294 21 6 457 8 448 3.72e-21 96.3
MS.gene08189.t1 AT5G07850 24.105 419 270 18 59 462 70 455 7.41e-21 95.1
MS.gene08189.t1 AT5G42830 25.061 407 258 18 59 449 64 439 7.47e-21 95.1
MS.gene08189.t1 AT5G47980 22.458 472 320 16 2 464 3 437 2.15e-19 90.9
MS.gene08189.t1 AT1G03940 24.033 491 298 21 6 457 11 465 2.07e-18 87.8
MS.gene08189.t1 AT4G15400 24.262 474 301 19 2 464 3 429 3.14e-18 87.0
MS.gene08189.t1 AT3G50300 25.522 431 245 22 59 459 59 443 1.27e-17 85.5
MS.gene08189.t1 AT5G39050 23.890 473 311 14 12 457 18 468 2.98e-17 84.3
MS.gene08189.t1 AT3G50280 25.260 289 180 13 136 417 13 272 4.63e-16 79.3
MS.gene08189.t1 AT1G03495 25.731 342 211 14 6 318 11 338 8.46e-16 79.0
MS.gene08189.t1 AT3G50290 25.214 234 152 9 136 362 19 236 8.40e-13 68.9

Find 87 sgRNAs with CRISPR-Local

Find 117 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTTTGCCTAAGAATTATTT+TGG 0.081602 7.3:+22763927 MS.gene08189:CDS
CCGTTTAATGAGACCGATTT+TGG 0.124040 7.3:+22764203 MS.gene08189:CDS
GCTAAAAGAAATGGACAATT+TGG 0.222470 7.3:+22764140 MS.gene08189:CDS
GATATCACTTTAAAAGATTT+AGG 0.281321 7.3:+22763327 MS.gene08189:CDS
CTGGGTCGTTCTGTTCTTGA+TGG 0.283808 7.3:+22763483 MS.gene08189:CDS
AAGTGCGCTTTGAAGGAAAT+TGG 0.321861 7.3:+22763850 MS.gene08189:CDS
CTATGTATGAACGGTGGATT+TGG 0.326559 7.3:-22763029 None:intergenic
CCTTGTGGGTCACTCACTCT+TGG 0.327948 7.3:+22763453 MS.gene08189:CDS
TTCCCAACCACATAACAAAA+AGG 0.329036 7.3:-22764008 None:intergenic
ACTCACTCTTGGTCTTGCTC+TGG 0.329835 7.3:+22763464 MS.gene08189:CDS
TAAAGTTGGTATCCATGGTT+AGG 0.353132 7.3:-22763117 None:intergenic
CAACTTCATTGCAACAATAA+AGG 0.357124 7.3:+22763279 MS.gene08189:CDS
CAACCAACAACATACAAATT+CGG 0.366121 7.3:-22764170 None:intergenic
AGAAGGATATGAATTTGTTA+AGG 0.368085 7.3:+22764099 MS.gene08189:CDS
GCATTCCCAAAATAATTCTT+AGG 0.370343 7.3:-22763933 None:intergenic
AGGTTATTAGTTGTTACATA+TGG 0.371511 7.3:+22763814 MS.gene08189:CDS
AACAAAGGAACATCTTCAAC+TGG 0.377562 7.3:-22763408 None:intergenic
ACCAAGGTTGTTAACCTTGT+TGG 0.383376 7.3:-22763879 None:intergenic
AGTTGAAGATGTTCCTTTGT+TGG 0.391503 7.3:+22763410 MS.gene08189:CDS
AGTGAGGGAAGCTATTAAAA+AGG 0.397885 7.3:+22764031 MS.gene08189:CDS
TATATTCTTGATGAAGCTAA+TGG 0.402079 7.3:+22764287 MS.gene08189:CDS
CCTGGTATTGTTAATTCTGA+TGG 0.403024 7.3:+22764251 MS.gene08189:CDS
ACTCACTACTATCCCTTTGC+AGG 0.409005 7.3:+22763219 MS.gene08189:CDS
AACAAAGTTCTATCATGAAA+TGG 0.413409 7.3:-22763579 None:intergenic
TTGACACTATCATCACAGTT+TGG 0.414530 7.3:-22764402 None:intergenic
CCTTGGTTAATTGTAGAAAC+AGG 0.414948 7.3:+22763895 MS.gene08189:CDS
GAAGGATATGAATTTGTTAA+GGG 0.417826 7.3:+22764100 MS.gene08189:CDS
GTTCTTGATGGTTCTTCTGC+TGG 0.417833 7.3:+22763495 MS.gene08189:CDS
AAACCTGATTGTATGGTTCC+TGG 0.419876 7.3:+22764233 MS.gene08189:CDS
ATCATGAAATGGAATCATAC+TGG 0.425406 7.3:-22763568 None:intergenic
ATAGTAGTGAGTGAGTGTTT+TGG 0.427197 7.3:-22763208 None:intergenic
CCAAAATCGGTCTCATTAAA+CGG 0.433375 7.3:-22764203 None:intergenic
AGTTTACCAAGTTTCCTTGT+GGG 0.443352 7.3:+22763439 MS.gene08189:CDS
CTCACTCTTGGTCTTGCTCT+GGG 0.445044 7.3:+22763465 MS.gene08189:CDS
ATTGTTAATTCTGATGGTAT+TGG 0.447032 7.3:+22764257 MS.gene08189:CDS
GACCCGAATTTGTATGTTGT+TGG 0.459492 7.3:+22764167 MS.gene08189:CDS
GTTTGTAAAGTGCGCTTTGA+AGG 0.462639 7.3:+22763843 MS.gene08189:CDS
TGTTTGTGAAGAGGTAAAGT+TGG 0.465289 7.3:-22763131 None:intergenic
AACCAACAACATACAAATTC+GGG 0.478369 7.3:-22764169 None:intergenic
TAATTCTGATGGTATTGGTA+AGG 0.485819 7.3:+22764262 MS.gene08189:CDS
CCAAAATAATTCTTAGGCAA+AGG 0.492931 7.3:-22763927 None:intergenic
CGAATTTGTATGTTGTTGGT+TGG 0.496564 7.3:+22764171 MS.gene08189:CDS
GGTGAATTTCAACATGGTAG+TGG 0.502907 7.3:-22763718 None:intergenic
TGCAATGAAGTTGTAGTAAG+TGG 0.507183 7.3:-22763269 None:intergenic
CAGTTTACCAAGTTTCCTTG+TGG 0.509783 7.3:+22763438 MS.gene08189:CDS
CAGCTTAATCTTCCTGCAAA+GGG 0.516823 7.3:-22763231 None:intergenic
CTGGTAGTTTCATTAGCTCG+TGG 0.518036 7.3:+22763514 MS.gene08189:CDS
TTATTAGTTGTTACATATGG+AGG 0.520920 7.3:+22763817 MS.gene08189:CDS
ACCAACAAGGTTAACAACCT+TGG 0.525311 7.3:+22763878 MS.gene08189:CDS
GTGGTTGGTGTTGTCTCTGA+AGG 0.528212 7.3:-22763057 None:intergenic
CCATCAGAATTAACAATACC+AGG 0.531351 7.3:-22764251 None:intergenic
GAGTGACCCACAAGGAAACT+TGG 0.531384 7.3:-22763445 None:intergenic
AAGAGATGAGTGATGATGAA+TGG 0.535532 7.3:-22763158 None:intergenic
AGAGGTAAAGTTGGTATCCA+TGG 0.536783 7.3:-22763122 None:intergenic
TCGTGGGCTAAACTTGCTAA+AGG 0.545668 7.3:+22763531 MS.gene08189:CDS
ATACCAGGAACCATACAATC+AGG 0.550344 7.3:-22764236 None:intergenic
AAATAATTCTTAGGCAAAGG+TGG 0.552771 7.3:-22763924 None:intergenic
CCAGCTTAATCTTCCTGCAA+AGG 0.553884 7.3:-22763232 None:intergenic
CTTGATGAAGCTAATGGTGA+TGG 0.559185 7.3:+22764293 MS.gene08189:CDS
AGGTTGTTAACCTTGTTGGT+TGG 0.562457 7.3:-22763875 None:intergenic
AAAGTTGGTATCCATGGTTA+GGG 0.563700 7.3:-22763116 None:intergenic
TGGTAGTTTCATTAGCTCGT+GGG 0.574135 7.3:+22763515 MS.gene08189:CDS
CCTTTGCAGGAAGATTAAGC+TGG 0.576850 7.3:+22763232 MS.gene08189:CDS
GAGCTTGAGTTGATCACAGA+GGG 0.580856 7.3:-22763094 None:intergenic
GAAAGAGAGAGTGTGGTGGT+TGG 0.587131 7.3:-22763072 None:intergenic
ACAGAGGGAAAGAGAGAGTG+TGG 0.591568 7.3:-22763079 None:intergenic
TGATTATGTAGCAAATCAGA+AGG 0.592162 7.3:+22764082 MS.gene08189:CDS
GTTATGTGGTTGGGAAAGTG+AGG 0.594320 7.3:+22764015 MS.gene08189:CDS
CAACTCAAGCTCCCTAACCA+TGG 0.599984 7.3:+22763105 MS.gene08189:CDS
AAAAGAAATGGACAATTTGG+TGG 0.606770 7.3:+22764143 MS.gene08189:CDS
CCTGTTTCTACAATTAACCA+AGG 0.607399 7.3:-22763895 None:intergenic
GAAAACGTGGTGGTAAATGA+AGG 0.612694 7.3:-22763614 None:intergenic
GAACAAGTTTGTGTCACCGT+AGG 0.630705 7.3:-22763963 None:intergenic
AGCCAACAACAATGAAAACA+AGG 0.634819 7.3:+22763770 MS.gene08189:CDS
GTAAACTGAACAACCAACAA+AGG 0.642328 7.3:-22763423 None:intergenic
GAAATTGGAACCAACCAACA+AGG 0.652154 7.3:+22763865 MS.gene08189:CDS
AACATGGTAGTGGCAAAGAG+TGG 0.657016 7.3:-22763708 None:intergenic
ACAAAGGAACATCTTCAACT+GGG 0.664068 7.3:-22763407 None:intergenic
AACAATGAAAACAAGGTGCG+AGG 0.665020 7.3:+22763777 MS.gene08189:CDS
TTATGTGGTTGGGAAAGTGA+GGG 0.670881 7.3:+22764016 MS.gene08189:CDS
CCAAGAGTGAGTGACCCACA+AGG 0.679354 7.3:-22763453 None:intergenic
AAGGTGTAACTATGTATGAA+CGG 0.693375 7.3:-22763038 None:intergenic
GAGGGAAAGAGAGAGTGTGG+TGG 0.702222 7.3:-22763076 None:intergenic
AACTCATGATGATGAAAACG+TGG 0.716513 7.3:-22763627 None:intergenic
GTGTAACTATGTATGAACGG+TGG 0.717757 7.3:-22763035 None:intergenic
TCATGATGATGAAAACGTGG+TGG 0.758242 7.3:-22763624 None:intergenic
GGAGCTTGAGTTGATCACAG+AGG 0.762986 7.3:-22763095 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATAATTTTGCTAAAAGAAA+TGG + chr7.3:22764131-22764150 MS.gene08189:CDS 15.0%
!!! AAGTTTTTCAACTTGTTTTT+TGG - chr7.3:22763742-22763761 None:intergenic 20.0%
!!! GATATCACTTTAAAAGATTT+AGG + chr7.3:22763327-22763346 MS.gene08189:CDS 20.0%
!!! TAATTTTTGGAACAAGTTTT+TGG - chr7.3:22763377-22763396 None:intergenic 20.0%
! AACAAAGTTCTATCATGAAA+TGG - chr7.3:22763582-22763601 None:intergenic 25.0%
! AGGTTATTAGTTGTTACATA+TGG + chr7.3:22763814-22763833 MS.gene08189:CDS 25.0%
! ATTGTTAATTCTGATGGTAT+TGG + chr7.3:22764257-22764276 MS.gene08189:CDS 25.0%
! TATATTCTTGATGAAGCTAA+TGG + chr7.3:22764287-22764306 MS.gene08189:CDS 25.0%
! TTATTAGTTGTTACATATGG+AGG + chr7.3:22763817-22763836 MS.gene08189:CDS 25.0%
!! ATTACTACTATTTTCCCAAA+GGG - chr7.3:22763664-22763683 None:intergenic 25.0%
!! CTTTGCCTAAGAATTATTTT+GGG + chr7.3:22763928-22763947 MS.gene08189:CDS 25.0%
!! TATTACTACTATTTTCCCAA+AGG - chr7.3:22763665-22763684 None:intergenic 25.0%
!!! AGAAGGATATGAATTTGTTA+AGG + chr7.3:22764099-22764118 MS.gene08189:CDS 25.0%
!!! GAAGGATATGAATTTGTTAA+GGG + chr7.3:22764100-22764119 MS.gene08189:CDS 25.0%
!!! TTTTAAAGTGATATCACATG+TGG - chr7.3:22763322-22763341 None:intergenic 25.0%
!!! TTTTTTGGTGAATTTCAACA+TGG - chr7.3:22763727-22763746 None:intergenic 25.0%
AAAAGAAATGGACAATTTGG+TGG + chr7.3:22764143-22764162 MS.gene08189:CDS 30.0%
AAATAATTCTTAGGCAAAGG+TGG - chr7.3:22763927-22763946 None:intergenic 30.0%
AACCAACAACATACAAATTC+GGG - chr7.3:22764172-22764191 None:intergenic 30.0%
AAGGTGTAACTATGTATGAA+CGG - chr7.3:22763041-22763060 None:intergenic 30.0%
ATCATGAAATGGAATCATAC+TGG - chr7.3:22763571-22763590 None:intergenic 30.0%
CAACCAACAACATACAAATT+CGG - chr7.3:22764173-22764192 None:intergenic 30.0%
CAACTTCATTGCAACAATAA+AGG + chr7.3:22763279-22763298 MS.gene08189:CDS 30.0%
CCAAAATAATTCTTAGGCAA+AGG - chr7.3:22763930-22763949 None:intergenic 30.0%
GCATTCCCAAAATAATTCTT+AGG - chr7.3:22763936-22763955 None:intergenic 30.0%
GCTAAAAGAAATGGACAATT+TGG + chr7.3:22764140-22764159 MS.gene08189:CDS 30.0%
TGATTATGTAGCAAATCAGA+AGG + chr7.3:22764082-22764101 MS.gene08189:CDS 30.0%
! CCTTTGCCTAAGAATTATTT+TGG + chr7.3:22763927-22763946 MS.gene08189:CDS 30.0%
! GGGACATCATAGTTAATTTT+TGG - chr7.3:22763390-22763409 None:intergenic 30.0%
! TTACTACTATTTTCCCAAAG+GGG - chr7.3:22763663-22763682 None:intergenic 30.0%
!! AATGGTTTTTGTTTGTGAAG+AGG - chr7.3:22763143-22763162 None:intergenic 30.0%
!! GAAGTTGTTTTATGAGGATA+TGG + chr7.3:22764364-22764383 MS.gene08189:CDS 30.0%
!! GTTGAAGAAGTTGTTTTATG+AGG + chr7.3:22764358-22764377 MS.gene08189:CDS 30.0%
!! TAATTCTGATGGTATTGGTA+AGG + chr7.3:22764262-22764281 MS.gene08189:CDS 30.0%
AACAAAGGAACATCTTCAAC+TGG - chr7.3:22763411-22763430 None:intergenic 35.0%
ACAAAGGAACATCTTCAACT+GGG - chr7.3:22763410-22763429 None:intergenic 35.0%
AGCCAACAACAATGAAAACA+AGG + chr7.3:22763770-22763789 MS.gene08189:CDS 35.0%
AGTGAGGGAAGCTATTAAAA+AGG + chr7.3:22764031-22764050 MS.gene08189:CDS 35.0%
AGTTGAAGATGTTCCTTTGT+TGG + chr7.3:22763410-22763429 MS.gene08189:CDS 35.0%
AGTTTACCAAGTTTCCTTGT+GGG + chr7.3:22763439-22763458 MS.gene08189:CDS 35.0%
CCAAAATCGGTCTCATTAAA+CGG - chr7.3:22764206-22764225 None:intergenic 35.0%
CCATCAGAATTAACAATACC+AGG - chr7.3:22764254-22764273 None:intergenic 35.0%
CCTGGTATTGTTAATTCTGA+TGG + chr7.3:22764251-22764270 MS.gene08189:CDS 35.0%
CCTTGGTTAATTGTAGAAAC+AGG + chr7.3:22763895-22763914 MS.gene08189:CDS 35.0%
CGAATTTGTATGTTGTTGGT+TGG + chr7.3:22764171-22764190 MS.gene08189:CDS 35.0%
GTAAACTGAACAACCAACAA+AGG - chr7.3:22763426-22763445 None:intergenic 35.0%
TGCAATGAAGTTGTAGTAAG+TGG - chr7.3:22763272-22763291 None:intergenic 35.0%
TGTTTGTGAAGAGGTAAAGT+TGG - chr7.3:22763134-22763153 None:intergenic 35.0%
TTCCCAACCACATAACAAAA+AGG - chr7.3:22764011-22764030 None:intergenic 35.0%
! AACTCATGATGATGAAAACG+TGG - chr7.3:22763630-22763649 None:intergenic 35.0%
! AAGAGATGAGTGATGATGAA+TGG - chr7.3:22763161-22763180 None:intergenic 35.0%
! ATAGTAGTGAGTGAGTGTTT+TGG - chr7.3:22763211-22763230 None:intergenic 35.0%
!! AAAGTTGGTATCCATGGTTA+GGG - chr7.3:22763119-22763138 None:intergenic 35.0%
!! AAGCCTTTTTGTTATGTGGT+TGG + chr7.3:22764005-22764024 MS.gene08189:CDS 35.0%
!! AGCCTTTTTGTTATGTGGTT+GGG + chr7.3:22764006-22764025 MS.gene08189:CDS 35.0%
!! CCTGTTTCTACAATTAACCA+AGG - chr7.3:22763898-22763917 None:intergenic 35.0%
!! CGTTTAATGAGACCGATTTT+GGG + chr7.3:22764204-22764223 MS.gene08189:CDS 35.0%
!! GAACAAGCCTTTTTGTTATG+TGG + chr7.3:22764001-22764020 MS.gene08189:CDS 35.0%
!! TAAAGTTGGTATCCATGGTT+AGG - chr7.3:22763120-22763139 None:intergenic 35.0%
!!! CACCTTGTTTTCATTGTTGT+TGG - chr7.3:22763775-22763794 None:intergenic 35.0%
AAACCTGATTGTATGGTTCC+TGG + chr7.3:22764233-22764252 MS.gene08189:CDS 40.0%
AACAATGAAAACAAGGTGCG+AGG + chr7.3:22763777-22763796 MS.gene08189:CDS 40.0%
ACCAACAAGGTTAACAACCT+TGG + chr7.3:22763878-22763897 MS.gene08189:CDS 40.0%
ACCAAGGTTGTTAACCTTGT+TGG - chr7.3:22763882-22763901 None:intergenic 40.0%
AGGTTGTTAACCTTGTTGGT+TGG - chr7.3:22763878-22763897 None:intergenic 40.0%
ATACCAGGAACCATACAATC+AGG - chr7.3:22764239-22764258 None:intergenic 40.0%
CAGCTTAATCTTCCTGCAAA+GGG - chr7.3:22763234-22763253 None:intergenic 40.0%
CAGTTTACCAAGTTTCCTTG+TGG + chr7.3:22763438-22763457 MS.gene08189:CDS 40.0%
CTTGATGAAGCTAATGGTGA+TGG + chr7.3:22764293-22764312 MS.gene08189:CDS 40.0%
GAAAACGTGGTGGTAAATGA+AGG - chr7.3:22763617-22763636 None:intergenic 40.0%
GAAATTGGAACCAACCAACA+AGG + chr7.3:22763865-22763884 MS.gene08189:CDS 40.0%
GACCCGAATTTGTATGTTGT+TGG + chr7.3:22764167-22764186 MS.gene08189:CDS 40.0%
GGTGAATTTCAACATGGTAG+TGG - chr7.3:22763721-22763740 None:intergenic 40.0%
GTGTAACTATGTATGAACGG+TGG - chr7.3:22763038-22763057 None:intergenic 40.0%
GTTTGTAAAGTGCGCTTTGA+AGG + chr7.3:22763843-22763862 MS.gene08189:CDS 40.0%
TGGTAGTTTCATTAGCTCGT+GGG + chr7.3:22763515-22763534 MS.gene08189:CDS 40.0%
TTATGTGGTTGGGAAAGTGA+GGG + chr7.3:22764016-22764035 MS.gene08189:CDS 40.0%
! CTATGTATGAACGGTGGATT+TGG - chr7.3:22763032-22763051 None:intergenic 40.0%
! TCATGATGATGAAAACGTGG+TGG - chr7.3:22763627-22763646 None:intergenic 40.0%
! TGGGAATGCAACTTTTCCTA+CGG + chr7.3:22763947-22763966 MS.gene08189:CDS 40.0%
!! AAGTGCGCTTTGAAGGAAAT+TGG + chr7.3:22763850-22763869 MS.gene08189:CDS 40.0%
!! AGAGGTAAAGTTGGTATCCA+TGG - chr7.3:22763125-22763144 None:intergenic 40.0%
!! CCGTTTAATGAGACCGATTT+TGG + chr7.3:22764203-22764222 MS.gene08189:CDS 40.0%
!! TAATGAGACCGATTTTGGGT+GGG + chr7.3:22764208-22764227 MS.gene08189:CDS 40.0%
!! TTAATGAGACCGATTTTGGG+TGG + chr7.3:22764207-22764226 MS.gene08189:CDS 40.0%
!!! GCGAGGTTATACTAGTTTTG+AGG + chr7.3:22763794-22763813 MS.gene08189:CDS 40.0%
ACTCACTACTATCCCTTTGC+AGG + chr7.3:22763219-22763238 MS.gene08189:CDS 45.0%
CCAGCTTAATCTTCCTGCAA+AGG - chr7.3:22763235-22763254 None:intergenic 45.0%
CCTTTGCAGGAAGATTAAGC+TGG + chr7.3:22763232-22763251 MS.gene08189:CDS 45.0%
CTGGTAGTTTCATTAGCTCG+TGG + chr7.3:22763514-22763533 MS.gene08189:CDS 45.0%
GAACAAGTTTGTGTCACCGT+AGG - chr7.3:22763966-22763985 None:intergenic 45.0%
GTTATGTGGTTGGGAAAGTG+AGG + chr7.3:22764015-22764034 MS.gene08189:CDS 45.0%
! CTACTATTTTCCCAAAGGGG+TGG - chr7.3:22763660-22763679 None:intergenic 45.0%
! GTTCTTGATGGTTCTTCTGC+TGG + chr7.3:22763495-22763514 MS.gene08189:CDS 45.0%
!! AACATGGTAGTGGCAAAGAG+TGG - chr7.3:22763711-22763730 None:intergenic 45.0%
!! AATGAGACCGATTTTGGGTG+GGG + chr7.3:22764209-22764228 MS.gene08189:CDS 45.0%
!! GAGCTTGAGTTGATCACAGA+GGG - chr7.3:22763097-22763116 None:intergenic 45.0%
!! TCGTGGGCTAAACTTGCTAA+AGG + chr7.3:22763531-22763550 MS.gene08189:CDS 45.0%
!!! AGTGAGTGTTTTGGAGAGAG+AGG - chr7.3:22763202-22763221 None:intergenic 45.0%
ACAGAGGGAAAGAGAGAGTG+TGG - chr7.3:22763082-22763101 None:intergenic 50.0%
AGGTTTCCCCCACCCAAAAT+CGG - chr7.3:22764219-22764238 None:intergenic 50.0%
CAACTCAAGCTCCCTAACCA+TGG + chr7.3:22763105-22763124 MS.gene08189:CDS 50.0%
CTGGGTCGTTCTGTTCTTGA+TGG + chr7.3:22763483-22763502 MS.gene08189:CDS 50.0%
GAGTGACCCACAAGGAAACT+TGG - chr7.3:22763448-22763467 None:intergenic 50.0%
GTGGGGGAAACCTGATTGTA+TGG + chr7.3:22764226-22764245 MS.gene08189:CDS 50.0%
! AGTTTTCTCCACCACCCCTT+TGG + chr7.3:22763646-22763665 MS.gene08189:CDS 50.0%
! CTATTTTCCCAAAGGGGTGG+TGG - chr7.3:22763657-22763676 None:intergenic 50.0%
! GAAAGAGAGAGTGTGGTGGT+TGG - chr7.3:22763075-22763094 None:intergenic 50.0%
! GTTTTCTCCACCACCCCTTT+GGG + chr7.3:22763647-22763666 MS.gene08189:CDS 50.0%
!! ACTCACTCTTGGTCTTGCTC+TGG + chr7.3:22763464-22763483 MS.gene08189:CDS 50.0%
!! ATGAGACCGATTTTGGGTGG+GGG + chr7.3:22764210-22764229 MS.gene08189:CDS 50.0%
!! CTCACTCTTGGTCTTGCTCT+GGG + chr7.3:22763465-22763484 MS.gene08189:CDS 50.0%
!! GGAGCTTGAGTTGATCACAG+AGG - chr7.3:22763098-22763117 None:intergenic 50.0%
!! GTGGTTGGTGTTGTCTCTGA+AGG - chr7.3:22763060-22763079 None:intergenic 50.0%
CCAAGAGTGAGTGACCCACA+AGG - chr7.3:22763456-22763475 None:intergenic 55.0%
CCTTGTGGGTCACTCACTCT+TGG + chr7.3:22763453-22763472 MS.gene08189:CDS 55.0%
! GAGGGAAAGAGAGAGTGTGG+TGG - chr7.3:22763079-22763098 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr7.3 gene 22763018 22764412 22763018 ID=MS.gene08189
chr7.3 mRNA 22763018 22764412 22763018 ID=MS.gene08189.t1;Parent=MS.gene08189
chr7.3 exon 22763018 22764412 22763018 ID=MS.gene08189.t1.exon1;Parent=MS.gene08189.t1
chr7.3 CDS 22763018 22764412 22763018 ID=cds.MS.gene08189.t1;Parent=MS.gene08189.t1
Gene Sequence

>MS.gene08189

ATGGTGAACATCCAAATCCACCGTTCATACATAGTTACACCTTCAGAGACAACACCAACCACCACACTCTCTCTTTCCCTCTGTGATCAACTCAAGCTCCCTAACCATGGATACCAACTTTACCTCTTCACAAACAAAAACCATTCATCATCACTCATCTCTTCAACAAACATTCTCATAACCTCTCTCTCCAAAACACTCACTCACTACTATCCCTTTGCAGGAAGATTAAGCTGGATCAAACTACAAAACCACTTACTACAACTTCATTGCAACAATAAAGGTGTACAATTCTTAGAAGCCACATGTGATATCACTTTAAAAGATTTAGGTAGTTTTGATTCTACACATGTTGTCCAAAAACTTGTTCCAAAAATTAACTATGATGTCCCAGTTGAAGATGTTCCTTTGTTGGTTGTTCAGTTTACCAAGTTTCCTTGTGGGTCACTCACTCTTGGTCTTGCTCTGGGTCGTTCTGTTCTTGATGGTTCTTCTGCTGGTAGTTTCATTAGCTCGTGGGCTAAACTTGCTAAAGGTGAGAGTTTAGATTCCAGTATGATTCCATTTCATGATAGAACTTTGTTAGATTCAAAGATCCTTCATTTACCACCACGTTTTCATCATCATGAGTTTTCTCCACCACCCCTTTGGGAAAATAGTAGTAATAACTCAACAATTGTGAGTAAAACACCACTCTTTGCCACTACCATGTTGAAATTCACCAAAAAACAAGTTGAAAAACTTAAGAACAAAGCCAACAACAATGAAAACAAGGTGCGAGGTTATACTAGTTTTGAGGTTATTAGTTGTTACATATGGAGGTGTGTTTGTAAAGTGCGCTTTGAAGGAAATTGGAACCAACCAACAAGGTTAACAACCTTGGTTAATTGTAGAAACAGGTTGAATCCACCTTTGCCTAAGAATTATTTTGGGAATGCAACTTTTCCTACGGTGACACAAACTTGTTCATTTGATGATGTTGTGAACAAGCCTTTTTGTTATGTGGTTGGGAAAGTGAGGGAAGCTATTAAAAAGGTGAATGATGAGTATGTAAGATCTGTTCTTGATTATGTAGCAAATCAGAAGGATATGAATTTGTTAAGGGACAAGTTTTATAATTTTGCTAAAAGAAATGGACAATTTGGTGGTGACCCGAATTTGTATGTTGTTGGTTGGACTAATTTTCCGTTTAATGAGACCGATTTTGGGTGGGGGAAACCTGATTGTATGGTTCCTGGTATTGTTAATTCTGATGGTATTGGTAAGGCTTATATTCTTGATGAAGCTAATGGTGATGGCTTTGTTGTAAGTGTTTGTTTGCAATCTTTTCATATAGATGCGTTGAAGAAGTTGTTTTATGAGGATATGGAGATGATCACTAGTTCCAAACTGTGA
Protein sequence

>MS.gene08189.t1

MVNIQIHRSYIVTPSETTPTTTLSLSLCDQLKLPNHGYQLYLFTNKNHSSSLISSTNILITSLSKTLTHYYPFAGRLSWIKLQNHLLQLHCNNKGVQFLEATCDITLKDLGSFDSTHVVQKLVPKINYDVPVEDVPLLVVQFTKFPCGSLTLGLALGRSVLDGSSAGSFISSWAKLAKGESLDSSMIPFHDRTLLDSKILHLPPRFHHHEFSPPPLWENSSNNSTIVSKTPLFATTMLKFTKKQVEKLKNKANNNENKVRGYTSFEVISCYIWRCVCKVRFEGNWNQPTRLTTLVNCRNRLNPPLPKNYFGNATFPTVTQTCSFDDVVNKPFCYVVGKVREAIKKVNDEYVRSVLDYVANQKDMNLLRDKFYNFAKRNGQFGGDPNLYVVGWTNFPFNETDFGWGKPDCMVPGIVNSDGIGKAYILDEANGDGFVVSVCLQSFHIDALKKLFYEDMEMITSSKL