AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene08349

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene08349.t1 MTR_5g019460 44.218 294 148 6 47 329 77 365 2.73e-78 244
MS.gene08349.t1 MTR_8g078070 41.695 295 152 8 48 329 79 366 5.98e-74 233
MS.gene08349.t1 MTR_5g092910 31.674 221 133 4 53 263 46 258 3.29e-24 100
MS.gene08349.t1 MTR_2g072470 25.116 215 144 6 61 268 113 317 4.95e-13 70.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene08349.t1 AT4G38460 64.605 291 93 2 45 335 42 322 1.24e-130 376
MS.gene08349.t1 AT4G36810 44.554 303 148 7 42 329 68 365 7.46e-82 253
MS.gene08349.t1 AT2G18620 39.093 353 187 9 1 333 1 345 3.52e-81 251
MS.gene08349.t1 AT2G18640 43.333 300 150 10 48 334 79 371 2.21e-71 226
MS.gene08349.t1 AT2G23800 40.667 300 158 10 48 334 83 375 2.05e-66 214
MS.gene08349.t1 AT3G20160 39.677 310 160 9 43 333 41 342 4.05e-66 212
MS.gene08349.t1 AT3G14550 42.424 297 150 7 48 329 64 354 5.62e-64 207
MS.gene08349.t1 AT3G14550 42.424 297 150 7 48 329 64 354 5.62e-64 207
MS.gene08349.t1 AT3G14550 42.424 297 150 7 48 329 64 354 5.62e-64 207
MS.gene08349.t1 AT3G20160 39.535 301 145 9 43 333 41 314 1.36e-63 204
MS.gene08349.t1 AT3G14530 42.761 297 149 7 48 329 64 354 6.84e-63 204
MS.gene08349.t1 AT3G14510 42.599 277 138 7 68 329 8 278 1.28e-62 201
MS.gene08349.t1 AT3G29430 41.231 325 166 11 24 329 33 351 9.48e-62 201
MS.gene08349.t1 AT3G32040 42.712 295 152 7 48 329 64 354 5.46e-61 199
MS.gene08349.t1 AT1G49530 39.672 305 157 8 52 333 34 334 7.73e-60 196
MS.gene08349.t1 AT1G17050 26.490 151 102 3 119 268 171 313 1.18e-11 65.9

Find 118 sgRNAs with CRISPR-Local

Find 148 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGAGTTATGTAGAGGTTTA+TGG 0.097267 4.1:-16981939 MS.gene08349:CDS
TTGGATGGATTTGAGAAGTA+TGG 0.258489 4.1:-16981861 MS.gene08349:CDS
AGCAAAGGCTTTGGAGGAAT+TGG 0.259488 4.1:-16981880 MS.gene08349:CDS
CGGTGTTGACATGGCAATTC+TGG 0.263672 4.1:-16982336 MS.gene08349:CDS
TGCTATTGTAGATGATATTT+TGG 0.266530 4.1:-16982009 MS.gene08349:CDS
TGTATCAATCCAACTGCATT+TGG 0.293189 4.1:+16982152 None:intergenic
TCTGATTAATCTGCCCCATT+AGG 0.299471 4.1:+16983214 None:intergenic
GGCTCCACTGGAATGGCTGC+AGG 0.305044 4.1:-16982200 MS.gene08349:CDS
AATGGCTGCAGGGCAGTTCT+TGG 0.308107 4.1:-16982189 MS.gene08349:CDS
AGAGTTATGTAGAGGTTTAT+GGG 0.313757 4.1:-16981938 MS.gene08349:CDS
GGCTGCTCCTTCTCCTTCTT+CGG 0.326678 4.1:-16983289 MS.gene08349:CDS
GGCATTGTCGCAATTGTAAA+AGG 0.335180 4.1:+16983375 None:intergenic
GGGCAGTTCTTGGACCTTGA+AGG 0.335310 4.1:-16982179 MS.gene08349:CDS
CGTAATTCACAAAACTATAG+AGG 0.352365 4.1:+16981823 None:intergenic
GGCAGTTCTTGGACCTTGAA+GGG 0.352960 4.1:-16982178 MS.gene08349:CDS
CTGATTAATCTGCCCCATTA+GGG 0.371204 4.1:+16983215 None:intergenic
GGATGAGATTCGGAGACTTT+CGG 0.384911 4.1:+16983331 None:intergenic
TGCCATGTCAACACCGTAGA+TGG 0.385514 4.1:+16982343 None:intergenic
CCGTAGATGGCGTGGTTTGA+GGG 0.385791 4.1:+16982356 None:intergenic
CAAGCAGAGATACACATAAC+AGG 0.399894 4.1:+16983099 None:intergenic
TTCCCATCTTTATCTCCCTC+AGG 0.406329 4.1:+16981972 None:intergenic
GACGTATTGGACTTCCCTAA+TGG 0.407597 4.1:-16983229 MS.gene08349:CDS
ACTTTGATTTGAAGACGTAT+TGG 0.408292 4.1:-16983242 MS.gene08349:CDS
ACACGAAGTACACGGGGTTC+AGG 0.414466 4.1:+16982248 None:intergenic
GTATCAATCCAACTGCATTT+GGG 0.418171 4.1:+16982153 None:intergenic
ATAGAGAGACTGAGGAGGTA+TGG 0.421597 4.1:-16982053 MS.gene08349:CDS
AGAACTTAGAGCAAAGGCTT+TGG 0.423144 4.1:-16981889 MS.gene08349:CDS
GCCCGCTGTGTGGGCTCCAC+TGG 0.424889 4.1:-16982212 MS.gene08349:CDS
ACGTATTGGACTTCCCTAAT+GGG 0.427242 4.1:-16983228 MS.gene08349:CDS
GAAAGAAGATTACCATTTCA+AGG 0.432327 4.1:+16983028 None:intergenic
GCAGCCATGGAGCAGCGAAT+TGG 0.434085 4.1:+16983306 None:intergenic
GGTGATGCACTCTTTCCCCT+TGG 0.434286 4.1:-16982311 MS.gene08349:CDS
TGCAGTGTGTGGAGGGTTGT+TGG 0.437777 4.1:-16982096 MS.gene08349:CDS
AGATGGCGTGGTTTGAGGGC+TGG 0.460200 4.1:+16982360 None:intergenic
ACTTAGAGCAAAGGCTTTGG+AGG 0.466634 4.1:-16981886 MS.gene08349:CDS
CGAATTGGAAGGATGAGATT+CGG 0.467097 4.1:+16983321 None:intergenic
TTGAGGGAACTGAACTTCAA+TGG 0.473114 4.1:+16983179 None:intergenic
CTGTGTGGGCTCCACTGGAA+TGG 0.474784 4.1:-16982207 MS.gene08349:CDS
TCTCTCTATCTCATCATCTT+CGG 0.478860 4.1:+16982067 None:intergenic
GCAGTTCTTGGACCTTGAAG+GGG 0.479193 4.1:-16982177 MS.gene08349:CDS
CACTGCATGTGCCCTTGAAA+TGG 0.480852 4.1:-16983040 MS.gene08349:intron
CGGTATTCTGCCCTTGCTAA+AGG 0.483034 4.1:-16983138 MS.gene08349:CDS
AAACCTGAGGGAGATAAAGA+TGG 0.484680 4.1:-16981975 MS.gene08349:CDS
CAATGCCCTCATTAAACATC+GGG 0.486811 4.1:-16983359 MS.gene08349:CDS
TCCAGTGGAGCCCACACAGC+GGG 0.487936 4.1:+16982211 None:intergenic
TTGACGAGGCCAGTAGATGA+TGG 0.492007 4.1:-16981779 None:intergenic
ACCGTAGATGGCGTGGTTTG+AGG 0.494704 4.1:+16982355 None:intergenic
CCCTCAAACCACGCCATCTA+CGG 0.495109 4.1:-16982356 MS.gene08349:CDS
AGAAGGAGAAGGAGCAGCCA+TGG 0.495191 4.1:+16983293 None:intergenic
AGGAGGTATGGTAGAGCTGT+TGG 0.495677 4.1:-16982041 MS.gene08349:CDS
CGTGGTTTGAGGGCTGGCCA+CGG 0.508134 4.1:+16982366 None:intergenic
CAGTTCTTGGACCTTGAAGG+GGG 0.508920 4.1:-16982176 MS.gene08349:CDS
CCTTCCAATTCGCTGCTCCA+TGG 0.514052 4.1:-16983310 MS.gene08349:CDS
GTGTGTGGAGGGTTGTTGGC+CGG 0.518361 4.1:-16982092 MS.gene08349:CDS
ATCGCTTAGCACCTTTAGCA+AGG 0.521626 4.1:+16983127 None:intergenic
TATCTCATCATCTTCGGCAC+CGG 0.522001 4.1:+16982073 None:intergenic
CGCCATCTACGGTGTTGACA+TGG 0.522018 4.1:-16982345 MS.gene08349:CDS
ATGATGAGATAGAGAGACTG+AGG 0.525344 4.1:-16982061 MS.gene08349:CDS
TTCAAGAACCCATCATCTAC+TGG 0.527028 4.1:+16981770 None:intergenic
GCTCCACTGGAATGGCTGCA+GGG 0.527665 4.1:-16982199 MS.gene08349:CDS
GTAATTCACAAAACTATAGA+GGG 0.531875 4.1:+16981824 None:intergenic
GGGCTGGCCACGGCGTGACA+AGG 0.532295 4.1:+16982376 None:intergenic
TGACAAGGAGTCATCCATGC+AGG 0.533253 4.1:+16982391 None:intergenic
ATAGCTTCATATATCTGTTG+AGG 0.536580 4.1:+16983162 None:intergenic
CAGAGGGAAGAGTTATGTAG+AGG 0.537549 4.1:-16981946 MS.gene08349:CDS
GATCGGATGGAAACCGAAGA+AGG 0.542003 4.1:+16983276 None:intergenic
GAAACACCCGATGTTTAATG+AGG 0.542457 4.1:+16983353 None:intergenic
GAGTTATGTAGAGGTTTATG+GGG 0.542842 4.1:-16981937 MS.gene08349:CDS
AACAATGTGTTGAAATCCAA+GGG 0.546111 4.1:+16982295 None:intergenic
CATTTCAAGGGCACATGCAG+TGG 0.547793 4.1:+16983041 None:intergenic
TAGCTTCATATATCTGTTGA+GGG 0.547993 4.1:+16983163 None:intergenic
AAGGCTTTGGAGGAATTGGA+TGG 0.548932 4.1:-16981876 MS.gene08349:CDS
GAGTGTTCTGCAGTGTGTGG+AGG 0.552684 4.1:-16982104 MS.gene08349:CDS
AAGGGCACATGCAGTGGGGA+AGG 0.558957 4.1:+16983047 None:intergenic
AAAGAAGATTACCATTTCAA+GGG 0.560180 4.1:+16983029 None:intergenic
GTACACGGGGTTCAGGTACA+AGG 0.565661 4.1:+16982255 None:intergenic
ATCATCATGTATCAATGAAG+CGG 0.568247 4.1:+16982418 None:intergenic
ATGCAGTGGGGAAGGCAGCA+AGG 0.568393 4.1:+16983055 None:intergenic
AACCTGAGGGAGATAAAGAT+GGG 0.571753 4.1:-16981974 MS.gene08349:CDS
ATGAGATAGAGAGACTGAGG+AGG 0.573192 4.1:-16982058 MS.gene08349:CDS
ATTTCAAGGGCACATGCAGT+GGG 0.576737 4.1:+16983042 None:intergenic
CAGCAATGACACGAAGTACA+CGG 0.576879 4.1:+16982240 None:intergenic
AGTGTTCTGCAGTGTGTGGA+GGG 0.579045 4.1:-16982103 MS.gene08349:CDS
GGCTGAAGAACTTAGAGCAA+AGG 0.579804 4.1:-16981895 MS.gene08349:CDS
CTGCCCTGCAGCCATTCCAG+TGG 0.580181 4.1:+16982196 None:intergenic
GCTGAGATAGCCCGCTGTGT+GGG 0.580286 4.1:-16982221 MS.gene08349:CDS
AGCAATGACACGAAGTACAC+GGG 0.582072 4.1:+16982241 None:intergenic
GTTGACATGGCAATTCTGGC+GGG 0.592172 4.1:-16982332 MS.gene08349:CDS
TCGCTTAGCACCTTTAGCAA+GGG 0.604739 4.1:+16983128 None:intergenic
CCATGGAGCAGCGAATTGGA+AGG 0.607622 4.1:+16983310 None:intergenic
ACATGATGATCTTCCCTGCA+TGG 0.608437 4.1:-16982405 MS.gene08349:CDS
ACAATGCCCTCATTAAACAT+CGG 0.612387 4.1:-16983360 MS.gene08349:CDS
GGGTTCAGGTACAAGGTCAG+AGG 0.613058 4.1:+16982262 None:intergenic
ATGGAAACCGAAGAAGGAGA+AGG 0.615829 4.1:+16983282 None:intergenic
TGTTGACATGGCAATTCTGG+CGG 0.616197 4.1:-16982333 MS.gene08349:CDS
AAACAATGTGTTGAAATCCA+AGG 0.617990 4.1:+16982294 None:intergenic
AAGCAGAGATACACATAACA+GGG 0.618552 4.1:+16983100 None:intergenic
GGAAGAGAGACTGAAACCTG+AGG 0.622933 4.1:-16981988 MS.gene08349:CDS
GATGACTCCTTGTCACGCCG+TGG 0.624688 4.1:-16982383 MS.gene08349:CDS
GACAAGGAGTCATCCATGCA+GGG 0.630249 4.1:+16982392 None:intergenic
GCGGAGTGTTCTGCAGTGTG+TGG 0.632086 4.1:-16982107 MS.gene08349:CDS
TGCTGAGATAGCCCGCTGTG+TGG 0.636542 4.1:-16982222 MS.gene08349:CDS
GAAGAGAGACTGAAACCTGA+GGG 0.638321 4.1:-16981987 MS.gene08349:CDS
AAACACCCGATGTTTAATGA+GGG 0.640367 4.1:+16983354 None:intergenic
TGTCAACACCGTAGATGGCG+TGG 0.643803 4.1:+16982348 None:intergenic
GCAGAGATACACATAACAGG+GGG 0.650726 4.1:+16983102 None:intergenic
AGAGATACACATAACAGGGG+GGG 0.650807 4.1:+16983104 None:intergenic
TAGAAGTTTCAATGTTGACG+AGG 0.655023 4.1:-16981793 MS.gene08349:CDS
CGTATTGGACTTCCCTAATG+GGG 0.660461 4.1:-16983227 MS.gene08349:CDS
GGTTCAGGTACAAGGTCAGA+GGG 0.665434 4.1:+16982263 None:intergenic
AACAGATATATGAAGCTATG+CGG 0.703508 4.1:-16983158 MS.gene08349:CDS
TAATTCACAAAACTATAGAG+GGG 0.709745 4.1:+16981825 None:intergenic
AGCAGAGATACACATAACAG+GGG 0.710591 4.1:+16983101 None:intergenic
TTCCAGTGGAGCCCACACAG+CGG 0.722527 4.1:+16982210 None:intergenic
ACAATGTGTTGAAATCCAAG+GGG 0.726426 4.1:+16982296 None:intergenic
CAGAGATACACATAACAGGG+GGG 0.735594 4.1:+16983103 None:intergenic
TTTCAAGGGCACATGCAGTG+GGG 0.742785 4.1:+16983043 None:intergenic
GCAATGACACGAAGTACACG+GGG 0.769728 4.1:+16982242 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTATGTTTGGTATTTCTTAT+TGG - chr4.1:16982603-16982622 MS.gene08349:intron 20.0%
!! TTGATACAAGATAAAAAGTT+TGG - chr4.1:16983026-16983045 MS.gene08349:intron 20.0%
!!! ATGATAAACTGTATGAATTT+TGG - chr4.1:16982691-16982710 MS.gene08349:intron 20.0%
! AAAGAAGATTACCATTTCAA+GGG + chr4.1:16982137-16982156 None:intergenic 25.0%
! AATATTCAGAGACACATTTA+AGG + chr4.1:16982584-16982603 None:intergenic 25.0%
! AATCAAAATTGGAAAGAGAA+AGG + chr4.1:16982365-16982384 None:intergenic 25.0%
! ACAATAGAATGAATATGAAG+TGG + chr4.1:16982170-16982189 None:intergenic 25.0%
! GATAGATAAAAGTAAAACTC+TGG + chr4.1:16982282-16982301 None:intergenic 25.0%
! GTAATTCACAAAACTATAGA+GGG + chr4.1:16983342-16983361 None:intergenic 25.0%
! TAATTCACAAAACTATAGAG+GGG + chr4.1:16983341-16983360 None:intergenic 25.0%
! TGTCTCTGAATATTTATGTT+TGG - chr4.1:16982590-16982609 MS.gene08349:intron 25.0%
!! AGATAAAAAGTTTGGTGAAA+TGG - chr4.1:16983034-16983053 MS.gene08349:intron 25.0%
!! TGCTATTGTAGATGATATTT+TGG - chr4.1:16983154-16983173 MS.gene08349:CDS 25.0%
!!! ATAGTACATACTTTGACTTT+TGG - chr4.1:16982529-16982548 MS.gene08349:intron 25.0%
AAACAATGTGTTGAAATCCA+AGG + chr4.1:16982872-16982891 None:intergenic 30.0%
AACAATGTGTTGAAATCCAA+GGG + chr4.1:16982871-16982890 None:intergenic 30.0%
AACAGATATATGAAGCTATG+CGG - chr4.1:16982005-16982024 MS.gene08349:CDS 30.0%
AAGAGTTATGTAGAGGTTTA+TGG - chr4.1:16983224-16983243 MS.gene08349:CDS 30.0%
AGAGTTATGTAGAGGTTTAT+GGG - chr4.1:16983225-16983244 MS.gene08349:CDS 30.0%
ATAAAGATGGGAAAAACAGA+GGG - chr4.1:16983201-16983220 MS.gene08349:CDS 30.0%
ATAGCTTCATATATCTGTTG+AGG + chr4.1:16982004-16982023 None:intergenic 30.0%
ATCATCATGTATCAATGAAG+CGG + chr4.1:16982748-16982767 None:intergenic 30.0%
CAGATACAACTAGCTAAAAA+TGG + chr4.1:16982503-16982522 None:intergenic 30.0%
CGTAATTCACAAAACTATAG+AGG + chr4.1:16983343-16983362 None:intergenic 30.0%
GAAAGAAGATTACCATTTCA+AGG + chr4.1:16982138-16982157 None:intergenic 30.0%
TAGCTTCATATATCTGTTGA+GGG + chr4.1:16982003-16982022 None:intergenic 30.0%
! TAAAAAGTTTGGTGAAATGG+CGG - chr4.1:16983037-16983056 MS.gene08349:intron 30.0%
!! ACTTTGATTTGAAGACGTAT+TGG - chr4.1:16981921-16981940 MS.gene08349:CDS 30.0%
!!! CTTATATTTTAGTTGCAAGC+AGG - chr4.1:16982716-16982735 MS.gene08349:intron 30.0%
AAACACCCGATGTTTAATGA+GGG + chr4.1:16981812-16981831 None:intergenic 35.0%
AAGCAGAGATACACATAACA+GGG + chr4.1:16982066-16982085 None:intergenic 35.0%
ACAATGCCCTCATTAAACAT+CGG - chr4.1:16981803-16981822 MS.gene08349:CDS 35.0%
ACAATGTGTTGAAATCCAAG+GGG + chr4.1:16982870-16982889 None:intergenic 35.0%
GAGTTATGTAGAGGTTTATG+GGG - chr4.1:16983226-16983245 MS.gene08349:CDS 35.0%
GATAAAGATGGGAAAAACAG+AGG - chr4.1:16983200-16983219 MS.gene08349:CDS 35.0%
GTATCAATCCAACTGCATTT+GGG + chr4.1:16983013-16983032 None:intergenic 35.0%
TAGAAGTTTCAATGTTGACG+AGG - chr4.1:16983370-16983389 MS.gene08349:CDS 35.0%
TCTCTCTATCTCATCATCTT+CGG + chr4.1:16983099-16983118 None:intergenic 35.0%
TGTATCAATCCAACTGCATT+TGG + chr4.1:16983014-16983033 None:intergenic 35.0%
! ATCTCTGCTTGTGAACTTTT+CGG - chr4.1:16982076-16982095 MS.gene08349:CDS 35.0%
! CAAAGTGAACAGCTTTTGAT+CGG + chr4.1:16981907-16981926 None:intergenic 35.0%
! GGATGAGCTTTCTTTTTCTT+TGG - chr4.1:16982335-16982354 MS.gene08349:CDS 35.0%
! TTGGATGGATTTGAGAAGTA+TGG - chr4.1:16983302-16983321 MS.gene08349:CDS 35.0%
AAACCTGAGGGAGATAAAGA+TGG - chr4.1:16983188-16983207 MS.gene08349:CDS 40.0%
AAAGAAAGCTCATCCTCTCA+CGG + chr4.1:16982330-16982349 None:intergenic 40.0%
AACCTGAGGGAGATAAAGAT+GGG - chr4.1:16983189-16983208 MS.gene08349:CDS 40.0%
ACGTATTGGACTTCCCTAAT+GGG - chr4.1:16981935-16981954 MS.gene08349:CDS 40.0%
AGAACTTAGAGCAAAGGCTT+TGG - chr4.1:16983274-16983293 MS.gene08349:CDS 40.0%
AGCAGAGATACACATAACAG+GGG + chr4.1:16982065-16982084 None:intergenic 40.0%
ATGATGAGATAGAGAGACTG+AGG - chr4.1:16983102-16983121 MS.gene08349:CDS 40.0%
CAAGCAGAGATACACATAAC+AGG + chr4.1:16982067-16982086 None:intergenic 40.0%
CAATGCCCTCATTAAACATC+GGG - chr4.1:16981804-16981823 MS.gene08349:CDS 40.0%
CGAATTGGAAGGATGAGATT+CGG + chr4.1:16981845-16981864 None:intergenic 40.0%
CTGATTAATCTGCCCCATTA+GGG + chr4.1:16981951-16981970 None:intergenic 40.0%
GAAACACCCGATGTTTAATG+AGG + chr4.1:16981813-16981832 None:intergenic 40.0%
GACAATGATAGACCAATCTC+TGG + chr4.1:16982230-16982249 None:intergenic 40.0%
GGCATTGTCGCAATTGTAAA+AGG + chr4.1:16981791-16981810 None:intergenic 40.0%
TCTGATTAATCTGCCCCATT+AGG + chr4.1:16981952-16981971 None:intergenic 40.0%
TTGAGGGAACTGAACTTCAA+TGG + chr4.1:16981987-16982006 None:intergenic 40.0%
ACATGATGATCTTCCCTGCA+TGG - chr4.1:16982758-16982777 MS.gene08349:intron 45.0%
AGAGATACACATAACAGGGG+GGG + chr4.1:16982062-16982081 None:intergenic 45.0%
AGCAATGACACGAAGTACAC+GGG + chr4.1:16982925-16982944 None:intergenic 45.0%
ATAGAGAGACTGAGGAGGTA+TGG - chr4.1:16983110-16983129 MS.gene08349:CDS 45.0%
ATCGCTTAGCACCTTTAGCA+AGG + chr4.1:16982039-16982058 None:intergenic 45.0%
ATGAGATAGAGAGACTGAGG+AGG - chr4.1:16983105-16983124 MS.gene08349:CDS 45.0%
ATGGAAACCGAAGAAGGAGA+AGG + chr4.1:16981884-16981903 None:intergenic 45.0%
CAGAGATACACATAACAGGG+GGG + chr4.1:16982063-16982082 None:intergenic 45.0%
CAGAGGGAAGAGTTATGTAG+AGG - chr4.1:16983217-16983236 MS.gene08349:CDS 45.0%
CAGCAATGACACGAAGTACA+CGG + chr4.1:16982926-16982945 None:intergenic 45.0%
CGTATTGGACTTCCCTAATG+GGG - chr4.1:16981936-16981955 MS.gene08349:CDS 45.0%
GAAGAGAGACTGAAACCTGA+GGG - chr4.1:16983176-16983195 MS.gene08349:CDS 45.0%
GACGTATTGGACTTCCCTAA+TGG - chr4.1:16981934-16981953 MS.gene08349:CDS 45.0%
GCAGAGATACACATAACAGG+GGG + chr4.1:16982064-16982083 None:intergenic 45.0%
GGATGAGATTCGGAGACTTT+CGG + chr4.1:16981835-16981854 None:intergenic 45.0%
GGCTGAAGAACTTAGAGCAA+AGG - chr4.1:16983268-16983287 MS.gene08349:CDS 45.0%
TATCTCATCATCTTCGGCAC+CGG + chr4.1:16983093-16983112 None:intergenic 45.0%
TCGCTTAGCACCTTTAGCAA+GGG + chr4.1:16982038-16982057 None:intergenic 45.0%
TGTTGACATGGCAATTCTGG+CGG - chr4.1:16982830-16982849 MS.gene08349:intron 45.0%
TTCCCATCTTTATCTCCCTC+AGG + chr4.1:16983194-16983213 None:intergenic 45.0%
! ACTTAGAGCAAAGGCTTTGG+AGG - chr4.1:16983277-16983296 MS.gene08349:CDS 45.0%
! ATTTCAAGGGCACATGCAGT+GGG + chr4.1:16982124-16982143 None:intergenic 45.0%
! GTGAACAGCTTTTGATCGGA+TGG + chr4.1:16981903-16981922 None:intergenic 45.0%
! TCTGCTTGTGAACTTTTCGG+AGG - chr4.1:16982079-16982098 MS.gene08349:CDS 45.0%
!! AAGGCTTTGGAGGAATTGGA+TGG - chr4.1:16983287-16983306 MS.gene08349:CDS 45.0%
!! AGCAAAGGCTTTGGAGGAAT+TGG - chr4.1:16983283-16983302 MS.gene08349:CDS 45.0%
!!! TTATTTTAATAAATCAAAAT+TGG + chr4.1:16982376-16982395 None:intergenic 5.0%
AAAGCTCATCCTCTCACGGT+AGG + chr4.1:16982326-16982345 None:intergenic 50.0%
AGTGTTCTGCAGTGTGTGGA+GGG - chr4.1:16983060-16983079 MS.gene08349:CDS 50.0%
CACTGCATGTGCCCTTGAAA+TGG - chr4.1:16982123-16982142 MS.gene08349:CDS 50.0%
CAGTTCTTGGACCTTGAAGG+GGG - chr4.1:16982987-16983006 MS.gene08349:intron 50.0%
CGGTATTCTGCCCTTGCTAA+AGG - chr4.1:16982025-16982044 MS.gene08349:CDS 50.0%
GACAAGGAGTCATCCATGCA+GGG + chr4.1:16982774-16982793 None:intergenic 50.0%
GATCGGATGGAAACCGAAGA+AGG + chr4.1:16981890-16981909 None:intergenic 50.0%
GCAATGACACGAAGTACACG+GGG + chr4.1:16982924-16982943 None:intergenic 50.0%
GCAGTTCTTGGACCTTGAAG+GGG - chr4.1:16982986-16983005 MS.gene08349:intron 50.0%
GCTAAGAACTCGCCAGAGAT+TGG - chr4.1:16982215-16982234 MS.gene08349:CDS 50.0%
GGAAGAGAGACTGAAACCTG+AGG - chr4.1:16983175-16983194 MS.gene08349:CDS 50.0%
GGCAGTTCTTGGACCTTGAA+GGG - chr4.1:16982985-16983004 MS.gene08349:intron 50.0%
GGTTCAGGTACAAGGTCAGA+GGG + chr4.1:16982903-16982922 None:intergenic 50.0%
GGTTGAAAAAGCAGCAGAGG+TGG - chr4.1:16983247-16983266 MS.gene08349:CDS 50.0%
GTTGACATGGCAATTCTGGC+GGG - chr4.1:16982831-16982850 MS.gene08349:intron 50.0%
TCACGGTAGGCAGTTAGGAA+CGG + chr4.1:16982313-16982332 None:intergenic 50.0%
TGACAAGGAGTCATCCATGC+AGG + chr4.1:16982775-16982794 None:intergenic 50.0%
TGCCATGTCAACACCGTAGA+TGG + chr4.1:16982823-16982842 None:intergenic 50.0%
TGGGGTTGAAAAAGCAGCAG+AGG - chr4.1:16983244-16983263 MS.gene08349:CDS 50.0%
! AGGAGGTATGGTAGAGCTGT+TGG - chr4.1:16983122-16983141 MS.gene08349:CDS 50.0%
! CATTTCAAGGGCACATGCAG+TGG + chr4.1:16982125-16982144 None:intergenic 50.0%
! TTTCAAGGGCACATGCAGTG+GGG + chr4.1:16982123-16982142 None:intergenic 50.0%
!! CGGTGTTGACATGGCAATTC+TGG - chr4.1:16982827-16982846 MS.gene08349:intron 50.0%
AATGGCTGCAGGGCAGTTCT+TGG - chr4.1:16982974-16982993 MS.gene08349:intron 55.0%
ACACGAAGTACACGGGGTTC+AGG + chr4.1:16982918-16982937 None:intergenic 55.0%
ACCGTAGATGGCGTGGTTTG+AGG + chr4.1:16982811-16982830 None:intergenic 55.0%
AGAAGGAGAAGGAGCAGCCA+TGG + chr4.1:16981873-16981892 None:intergenic 55.0%
CCATGGAGCAGCGAATTGGA+AGG + chr4.1:16981856-16981875 None:intergenic 55.0%
CCCTCAAACCACGCCATCTA+CGG - chr4.1:16982807-16982826 MS.gene08349:intron 55.0%
CCGTAGATGGCGTGGTTTGA+GGG + chr4.1:16982810-16982829 None:intergenic 55.0%
CCTTCCAATTCGCTGCTCCA+TGG - chr4.1:16981853-16981872 MS.gene08349:CDS 55.0%
GAGTGTTCTGCAGTGTGTGG+AGG - chr4.1:16983059-16983078 MS.gene08349:CDS 55.0%
GGCTGCTCCTTCTCCTTCTT+CGG - chr4.1:16981874-16981893 MS.gene08349:CDS 55.0%
GGGCAGTTCTTGGACCTTGA+AGG - chr4.1:16982984-16983003 MS.gene08349:intron 55.0%
GGGTTCAGGTACAAGGTCAG+AGG + chr4.1:16982904-16982923 None:intergenic 55.0%
GGTGATGCACTCTTTCCCCT+TGG - chr4.1:16982852-16982871 MS.gene08349:intron 55.0%
GTACACGGGGTTCAGGTACA+AGG + chr4.1:16982911-16982930 None:intergenic 55.0%
TCCTAACTGCCTACCGTGAG+AGG - chr4.1:16982314-16982333 MS.gene08349:CDS 55.0%
TCCTCTCACGGTAGGCAGTT+AGG + chr4.1:16982318-16982337 None:intergenic 55.0%
TGCAGTGTGTGGAGGGTTGT+TGG - chr4.1:16983067-16983086 MS.gene08349:CDS 55.0%
TGTCAACACCGTAGATGGCG+TGG + chr4.1:16982818-16982837 None:intergenic 55.0%
!! CGCCATCTACGGTGTTGACA+TGG - chr4.1:16982818-16982837 MS.gene08349:intron 55.0%
AGATGGCGTGGTTTGAGGGC+TGG + chr4.1:16982806-16982825 None:intergenic 60.0%
ATGCAGTGGGGAAGGCAGCA+AGG + chr4.1:16982111-16982130 None:intergenic 60.0%
CTGCATTTGGGCCCCCTTCA+AGG + chr4.1:16983001-16983020 None:intergenic 60.0%
CTGTGTGGGCTCCACTGGAA+TGG - chr4.1:16982956-16982975 MS.gene08349:intron 60.0%
GAAGGGGGCCCAAATGCAGT+TGG - chr4.1:16983002-16983021 MS.gene08349:intron 60.0%
GATGACTCCTTGTCACGCCG+TGG - chr4.1:16982780-16982799 MS.gene08349:intron 60.0%
GCAGCCATGGAGCAGCGAAT+TGG + chr4.1:16981860-16981879 None:intergenic 60.0%
GCTCCACTGGAATGGCTGCA+GGG - chr4.1:16982964-16982983 MS.gene08349:intron 60.0%
GCTGAGATAGCCCGCTGTGT+GGG - chr4.1:16982942-16982961 MS.gene08349:intron 60.0%
GTGTGTGGAGGGTTGTTGGC+CGG - chr4.1:16983071-16983090 MS.gene08349:CDS 60.0%
TGCTGAGATAGCCCGCTGTG+TGG - chr4.1:16982941-16982960 MS.gene08349:intron 60.0%
TTCCAGTGGAGCCCACACAG+CGG + chr4.1:16982956-16982975 None:intergenic 60.0%
! AAGGGCACATGCAGTGGGGA+AGG + chr4.1:16982119-16982138 None:intergenic 60.0%
! GCGGAGTGTTCTGCAGTGTG+TGG - chr4.1:16983056-16983075 MS.gene08349:CDS 60.0%
CTGCCCTGCAGCCATTCCAG+TGG + chr4.1:16982970-16982989 None:intergenic 65.0%
GGCTCCACTGGAATGGCTGC+AGG - chr4.1:16982963-16982982 MS.gene08349:intron 65.0%
TCCAGTGGAGCCCACACAGC+GGG + chr4.1:16982955-16982974 None:intergenic 65.0%
!! CGTGGTTTGAGGGCTGGCCA+CGG + chr4.1:16982800-16982819 None:intergenic 65.0%
GCCCGCTGTGTGGGCTCCAC+TGG - chr4.1:16982951-16982970 MS.gene08349:intron 75.0%
!! GGGCTGGCCACGGCGTGACA+AGG + chr4.1:16982790-16982809 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 16981782 16983403 16981782 ID=MS.gene08349
chr4.1 mRNA 16981782 16983403 16981782 ID=MS.gene08349.t1;Parent=MS.gene08349
chr4.1 exon 16983041 16983403 16983041 ID=MS.gene08349.t1.exon1;Parent=MS.gene08349.t1
chr4.1 CDS 16983041 16983403 16983041 ID=cds.MS.gene08349.t1;Parent=MS.gene08349.t1
chr4.1 exon 16981782 16982447 16981782 ID=MS.gene08349.t1.exon2;Parent=MS.gene08349.t1
chr4.1 CDS 16981782 16982447 16981782 ID=cds.MS.gene08349.t1;Parent=MS.gene08349.t1
Gene Sequence

>MS.gene08349

ATGGCTCCTTTTACAATTGCGACAATGCCCTCATTAAACATCGGGTGTTTCCGAAAGTCTCCGAATCTCATCCTTCCAATTCGCTGCTCCATGGCTGCTCCTTCTCCTTCTTCGGTTTCCATCCGATCAAAAGCTGTTCACTTTGATTTGAAGACGTATTGGACTTCCCTAATGGGGCAGATTAATCAGAAGCTCGATGAAGCCATTGAAGTTCAGTTCCCTCAACAGATATATGAAGCTATGCGGTATTCTGCCCTTGCTAAAGGTGCTAAGCGATCCCCCCCTGTTATGTGTATCTCTGCTTGTGAACTTTTCGGAGGCAGTCGCCTTGCTGCCTTCCCCACTGCATGTGCCCTTGAAATGGTAATCTTCTTTCTATAATCTGCCACTTCATATTCATTCTATTGTATTATGTTTTTAGTTTGTTTTTTATGCTAAGAACTCGCCAGAGATTGGTCTATCATTGTCACAAATTTATTTGTTATGTTGTTGAGCTACCAGAGTTTTACTTTTATCTATCAAATAAATCCGTTCCTAACTGCCTACCGTGAGAGGATGAGCTTTCTTTTTCTTTGGACTTCCTTTCTCTTTCCAATTTTGATTTATTAAAATAATAACATATGTTGCATATTTCTTCAAAAAAAAAAAAAAAAAAACATATGTTGCATGTTTAGTTGCAGTAATGCTGTGTTGCTCATATAAGTAACAGTTTTGCATTCCATTTTTAGCTAGTTGTATCTGCTTAAAATAGTACATACTTTGACTTTTGGATCATCTGTTAAGAATGATTGTAGCATTACCTTAAATGTGTCTCTGAATATTTATGTTTGGTATTTCTTATTGGACACAAAGTGAGATTTATGAAGCGTTTATATAATTATATGCACTTCAGATAAATAAATAATGATGATGATAAACTGTATGAATTTTGGAACTTATATTTTAGTTGCAAGCAGGTTCATGCCGCTTCATTGATACATGATGATCTTCCCTGCATGGATGACTCCTTGTCACGCCGTGGCCAGCCCTCAAACCACGCCATCTACGGTGTTGACATGGCAATTCTGGCGGGTGATGCACTCTTTCCCCTTGGATTTCAACACATTGTTTCACATACTCCCTCTGACCTTGTACCTGAACCCCGTGTACTTCGTGTCATTGCTGAGATAGCCCGCTGTGTGGGCTCCACTGGAATGGCTGCAGGGCAGTTCTTGGACCTTGAAGGGGGCCCAAATGCAGTTGGATTGATACAAGATAAAAAGTTTGGTGAAATGGCGGAGTGTTCTGCAGTGTGTGGAGGGTTGTTGGCCGGTGCCGAAGATGATGAGATAGAGAGACTGAGGAGGTATGGTAGAGCTGTTGGAGTATTGTATGCTATTGTAGATGATATTTTGGAAGAGAGACTGAAACCTGAGGGAGATAAAGATGGGAAAAACAGAGGGAAGAGTTATGTAGAGGTTTATGGGGTTGAAAAAGCAGCAGAGGTGGCTGAAGAACTTAGAGCAAAGGCTTTGGAGGAATTGGATGGATTTGAGAAGTATGGAGATCAAGTGTTGCCCCTCTATAGTTTTGTGAATTACGCTATTGATAGAAGTTTCAATGTTGACGAGGCCAGTAGATGA
Protein sequence

>MS.gene08349.t1

MAPFTIATMPSLNIGCFRKSPNLILPIRCSMAAPSPSSVSIRSKAVHFDLKTYWTSLMGQINQKLDEAIEVQFPQQIYEAMRYSALAKGAKRSPPVMCISACELFGGSRLAAFPTACALEMVHAASLIHDDLPCMDDSLSRRGQPSNHAIYGVDMAILAGDALFPLGFQHIVSHTPSDLVPEPRVLRVIAEIARCVGSTGMAAGQFLDLEGGPNAVGLIQDKKFGEMAECSAVCGGLLAGAEDDEIERLRRYGRAVGVLYAIVDDILEERLKPEGDKDGKNRGKSYVEVYGVEKAAEVAEELRAKALEELDGFEKYGDQVLPLYSFVNYAIDRSFNVDEASR