Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08422.t1 | XP_013457381.1 | 98.6 | 218 | 3 | 0 | 1 | 218 | 1 | 218 | 1.40E-123 | 452.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08422.t1 | Q6E2Z6 | 98.2 | 218 | 4 | 0 | 1 | 218 | 1 | 218 | 9.5e-126 | 450.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08422.t1 | A0A072UP31 | 98.6 | 218 | 3 | 0 | 1 | 218 | 1 | 218 | 9.8e-124 | 452.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene26698 | MS.gene08422 | PPI |
| MS.gene030247 | MS.gene08422 | PPI |
| MS.gene24767 | MS.gene08422 | PPI |
| MS.gene012180 | MS.gene08422 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08422.t1 | MTR_4g094720 | 98.624 | 218 | 3 | 0 | 1 | 218 | 1 | 218 | 1.85e-163 | 449 |
| MS.gene08422.t1 | MTR_7g105830 | 31.111 | 180 | 114 | 3 | 6 | 180 | 74 | 248 | 5.35e-23 | 94.0 |
| MS.gene08422.t1 | MTR_1g105090 | 30.000 | 180 | 116 | 3 | 6 | 180 | 76 | 250 | 4.76e-22 | 91.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08422.t1 | AT1G48130 | 72.273 | 220 | 54 | 2 | 1 | 218 | 1 | 215 | 3.08e-116 | 330 |
| MS.gene08422.t1 | AT5G06290 | 31.667 | 180 | 113 | 3 | 6 | 180 | 82 | 256 | 2.45e-24 | 97.4 |
| MS.gene08422.t1 | AT3G11630 | 32.222 | 180 | 112 | 3 | 6 | 180 | 75 | 249 | 1.69e-23 | 95.1 |
| MS.gene08422.t1 | AT5G06290 | 29.448 | 163 | 106 | 2 | 6 | 164 | 82 | 239 | 6.20e-20 | 85.1 |
Find 62 sgRNAs with CRISPR-Local
Find 73 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTGCTTAATGATTTCTCTTT+TGG | 0.116285 | 4.1:-17844711 | None:intergenic |
| GATATTTGTACCTTCTTATC+TGG | 0.262678 | 4.1:-17844806 | None:intergenic |
| TCCCCTGGCTTCCAGTTAGC+AGG | 0.316622 | 4.1:-17845005 | None:intergenic |
| ATGTTCCTCCCAGTTGTTGC+AGG | 0.342538 | 4.1:-17844921 | None:intergenic |
| CCATTGGAATCTTTCTCATC+AGG | 0.347454 | 4.1:-17844749 | None:intergenic |
| CCAAGCTCAGTGGTACAAAC+TGG | 0.350619 | 4.1:-17844405 | None:intergenic |
| TTTCTGTACCCTGCAACAAC+TGG | 0.357498 | 4.1:+17844912 | MS.gene08422:CDS |
| CATATCCTTAGCTTGTTCAT+TGG | 0.360719 | 4.1:-17845054 | None:intergenic |
| AAATTGCAACCCCTGCTAAC+TGG | 0.361486 | 4.1:+17844994 | MS.gene08422:CDS |
| TGCAGATCTTCCATCCAAAA+AGG | 0.376713 | 4.1:+17845097 | MS.gene08422:CDS |
| CTTGTGTAGTATCAACTTCA+AGG | 0.408006 | 4.1:-17844311 | None:intergenic |
| GCTTGTTCATTGGTAACATC+AGG | 0.425824 | 4.1:-17845044 | None:intergenic |
| ACAATATGAAGAGCCCTTGA+TGG | 0.428726 | 4.1:-17844779 | None:intergenic |
| ACCCCTGCTAACTGGAAGCC+AGG | 0.430708 | 4.1:+17845002 | MS.gene08422:CDS |
| TACCACTGAGCTTGGTAAGA+TGG | 0.430872 | 4.1:+17844413 | MS.gene08422:CDS |
| CCCCTGGCTTCCAGTTAGCA+GGG | 0.440132 | 4.1:-17845004 | None:intergenic |
| AAACGAGGAGTTATGCTGTT+AGG | 0.461288 | 4.1:+17844459 | MS.gene08422:CDS |
| CAATGGAAATTTACCATCAA+GGG | 0.465608 | 4.1:+17844766 | MS.gene08422:CDS |
| GGATAGTTCACCTTTGCACC+TGG | 0.476765 | 4.1:-17844674 | None:intergenic |
| AATATGCCAGGCCTTACTAT+AGG | 0.481380 | 4.1:+17844273 | None:intergenic |
| CCAATGGAAATTTACCATCA+AGG | 0.485151 | 4.1:+17844765 | MS.gene08422:CDS |
| CCTTGATGGTAAATTTCCAT+TGG | 0.485846 | 4.1:-17844765 | None:intergenic |
| ACCATTCTCTTCTCTCATCC+AGG | 0.497155 | 4.1:+17844372 | MS.gene08422:CDS |
| ATCACTTCTGTTCTGATACA+TGG | 0.498086 | 4.1:+17844349 | MS.gene08422:CDS |
| GTAGTATCAACTTCAAGGTC+AGG | 0.507580 | 4.1:-17844306 | None:intergenic |
| ATCAAGGGCTCTTCATATTG+TGG | 0.511782 | 4.1:+17844781 | MS.gene08422:CDS |
| CTGCAGTCTTAAAACCTTGA+GGG | 0.516211 | 4.1:-17845079 | None:intergenic |
| ACATCAGGAGAAATGACAAC+AGG | 0.519996 | 4.1:-17845029 | None:intergenic |
| CAAGCTCAGTGGTACAAACT+GGG | 0.522401 | 4.1:-17844404 | None:intergenic |
| TCAAGGGCTCTTCATATTGT+GGG | 0.523725 | 4.1:+17844782 | MS.gene08422:CDS |
| TGTTCCTCCCAGTTGTTGCA+GGG | 0.527210 | 4.1:-17844920 | None:intergenic |
| TTCTGTACCCTGCAACAACT+GGG | 0.528210 | 4.1:+17844913 | MS.gene08422:CDS |
| GAATTGTGTCACCTATAGTA+AGG | 0.528944 | 4.1:-17844284 | None:intergenic |
| CCAGTTTGTACCACTGAGCT+TGG | 0.529958 | 4.1:+17844405 | MS.gene08422:CDS |
| TCAAAACTAGAAAATATGCC+AGG | 0.532876 | 4.1:+17844261 | None:intergenic |
| CAAACTGGGGTGAAATCACC+TGG | 0.538828 | 4.1:-17844390 | None:intergenic |
| ATCTAGAATCTCACAAGGAG+TGG | 0.546589 | 4.1:+17844496 | MS.gene08422:CDS |
| CCCCTGCTAACTGGAAGCCA+GGG | 0.547614 | 4.1:+17845003 | MS.gene08422:CDS |
| GTGTCACCTATAGTAAGGCC+TGG | 0.556059 | 4.1:-17844279 | None:intergenic |
| CCCTGGCTTCCAGTTAGCAG+GGG | 0.564511 | 4.1:-17845003 | None:intergenic |
| ATGTATCAGAACAGAAGTGA+TGG | 0.570051 | 4.1:-17844347 | None:intergenic |
| TGTTACCAATGAACAAGCTA+AGG | 0.572171 | 4.1:+17845049 | MS.gene08422:CDS |
| TATTGTGGGACCAGATAAGA+AGG | 0.572882 | 4.1:+17844796 | MS.gene08422:CDS |
| GAAATGACAACAGGTTCCCC+TGG | 0.579339 | 4.1:-17845020 | None:intergenic |
| GTAAATGCAGCCAGGTGCAA+AGG | 0.582844 | 4.1:+17844664 | MS.gene08422:intron |
| TGCAACAACTGGGAGGAACA+TGG | 0.585961 | 4.1:+17844923 | MS.gene08422:CDS |
| CCTGATGAGAAAGATTCCAA+TGG | 0.589101 | 4.1:+17844749 | MS.gene08422:CDS |
| TCTGCAGTCTTAAAACCTTG+AGG | 0.590174 | 4.1:-17845080 | None:intergenic |
| GCTAAGGATATGTTCCCTCA+AGG | 0.596047 | 4.1:+17845065 | MS.gene08422:CDS |
| ACCTGGATGAGAGAAGAGAA+TGG | 0.604374 | 4.1:-17844373 | None:intergenic |
| GCAGGCACATTATCAAACAT+TGG | 0.624118 | 4.1:-17845135 | None:intergenic |
| GAACATGGATGAAGTGCTGA+GGG | 0.626828 | 4.1:+17844938 | MS.gene08422:CDS |
| AATCATTAAGCAACTCAACA+TGG | 0.634966 | 4.1:+17844721 | MS.gene08422:CDS |
| GGAACATGGATGAAGTGCTG+AGG | 0.641851 | 4.1:+17844937 | MS.gene08422:CDS |
| CTTGAAGTTGATACTACACA+AGG | 0.656492 | 4.1:+17844312 | MS.gene08422:CDS |
| GCTTCTGAGTTTAACAAACG+AGG | 0.664933 | 4.1:+17844444 | MS.gene08422:CDS |
| AGCCATCTTACCAAGCTCAG+TGG | 0.696126 | 4.1:-17844415 | None:intergenic |
| CCCTGCTAACTGGAAGCCAG+GGG | 0.697213 | 4.1:+17845004 | MS.gene08422:CDS |
| CATGGATGAAGTGCTGAGGG+TGG | 0.707751 | 4.1:+17844941 | MS.gene08422:CDS |
| TGATGATCTAGAATCTCACA+AGG | 0.709676 | 4.1:+17844491 | MS.gene08422:CDS |
| AAGCTCAGTGGTACAAACTG+GGG | 0.712192 | 4.1:-17844403 | None:intergenic |
| TGTACCCTGCAACAACTGGG+AGG | 0.722121 | 4.1:+17844916 | MS.gene08422:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAGAAAATAGGAAAACTTA+CGG | - | chr4.1:17844544-17844563 | None:intergenic | 20.0% |
| !! | ACAAATTAAACCATCATTTA+TGG | - | chr4.1:17844875-17844894 | None:intergenic | 20.0% |
| !! | GAAACATGCATAGAAAATTT+TGG | - | chr4.1:17844583-17844602 | None:intergenic | 25.0% |
| !! | TAAGAAGGTACAAATATCTA+TGG | + | chr4.1:17844811-17844830 | MS.gene08422:intron | 25.0% |
| !! | TGCTTAATGATTTCTCTTTT+GGG | - | chr4.1:17844713-17844732 | None:intergenic | 25.0% |
| !! | TTGCTTAATGATTTCTCTTT+TGG | - | chr4.1:17844714-17844733 | None:intergenic | 25.0% |
| !!! | CTTTTTTCTTCCATAAATGA+TGG | + | chr4.1:17844862-17844881 | MS.gene08422:intron | 25.0% |
| AATCATTAAGCAACTCAACA+TGG | + | chr4.1:17844721-17844740 | MS.gene08422:CDS | 30.0% | |
| ATGCATGTGGAAAAGAAAAT+AGG | - | chr4.1:17844555-17844574 | None:intergenic | 30.0% | |
| CAATGGAAATTTACCATCAA+GGG | + | chr4.1:17844766-17844785 | MS.gene08422:CDS | 30.0% | |
| GATATTTGTACCTTCTTATC+TGG | - | chr4.1:17844809-17844828 | None:intergenic | 30.0% | |
| !!! | CTTTTGGGATCACTAATTAT+TGG | - | chr4.1:17844698-17844717 | None:intergenic | 30.0% |
| !!! | TTTTTTATGTAAATGCAGCC+AGG | + | chr4.1:17844656-17844675 | MS.gene08422:intron | 30.0% |
| ATCACTTCTGTTCTGATACA+TGG | + | chr4.1:17844349-17844368 | MS.gene08422:CDS | 35.0% | |
| ATGTATCAGAACAGAAGTGA+TGG | - | chr4.1:17844350-17844369 | None:intergenic | 35.0% | |
| CCAATGGAAATTTACCATCA+AGG | + | chr4.1:17844765-17844784 | MS.gene08422:CDS | 35.0% | |
| CTTGTGTAGTATCAACTTCA+AGG | - | chr4.1:17844314-17844333 | None:intergenic | 35.0% | |
| GAATTGTGTCACCTATAGTA+AGG | - | chr4.1:17844287-17844306 | None:intergenic | 35.0% | |
| TGTTACCAATGAACAAGCTA+AGG | + | chr4.1:17845049-17845068 | MS.gene08422:CDS | 35.0% | |
| ! | CCTTGATGGTAAATTTCCAT+TGG | - | chr4.1:17844768-17844787 | None:intergenic | 35.0% |
| ! | CTAAGATACTCCTTTTTGGA+TGG | - | chr4.1:17845110-17845129 | None:intergenic | 35.0% |
| ! | GAACCTAAGATACTCCTTTT+TGG | - | chr4.1:17845114-17845133 | None:intergenic | 35.0% |
| ! | TGATGATCTAGAATCTCACA+AGG | + | chr4.1:17844491-17844510 | MS.gene08422:CDS | 35.0% |
| !! | AATTTTGGTATGCATGCATG+TGG | - | chr4.1:17844568-17844587 | None:intergenic | 35.0% |
| !! | CATATCCTTAGCTTGTTCAT+TGG | - | chr4.1:17845057-17845076 | None:intergenic | 35.0% |
| !! | CATCCAAAAAGGAGTATCTT+AGG | + | chr4.1:17845108-17845127 | MS.gene08422:CDS | 35.0% |
| !! | CTTGAAGTTGATACTACACA+AGG | + | chr4.1:17844312-17844331 | MS.gene08422:CDS | 35.0% |
| AAACGAGGAGTTATGCTGTT+AGG | + | chr4.1:17844459-17844478 | MS.gene08422:CDS | 40.0% | |
| ACAATATGAAGAGCCCTTGA+TGG | - | chr4.1:17844782-17844801 | None:intergenic | 40.0% | |
| ACATCAGGAGAAATGACAAC+AGG | - | chr4.1:17845032-17845051 | None:intergenic | 40.0% | |
| ATCAAGGGCTCTTCATATTG+TGG | + | chr4.1:17844781-17844800 | MS.gene08422:CDS | 40.0% | |
| CCATTGGAATCTTTCTCATC+AGG | - | chr4.1:17844752-17844771 | None:intergenic | 40.0% | |
| CCTGATGAGAAAGATTCCAA+TGG | + | chr4.1:17844749-17844768 | MS.gene08422:CDS | 40.0% | |
| CTGCAGTCTTAAAACCTTGA+GGG | - | chr4.1:17845082-17845101 | None:intergenic | 40.0% | |
| GCTTCTGAGTTTAACAAACG+AGG | + | chr4.1:17844444-17844463 | MS.gene08422:CDS | 40.0% | |
| GTAGTATCAACTTCAAGGTC+AGG | - | chr4.1:17844309-17844328 | None:intergenic | 40.0% | |
| TATTGTGGGACCAGATAAGA+AGG | + | chr4.1:17844796-17844815 | MS.gene08422:CDS | 40.0% | |
| TCAAGGGCTCTTCATATTGT+GGG | + | chr4.1:17844782-17844801 | MS.gene08422:CDS | 40.0% | |
| TCTGCAGTCTTAAAACCTTG+AGG | - | chr4.1:17845083-17845102 | None:intergenic | 40.0% | |
| TGCAGATCTTCCATCCAAAA+AGG | + | chr4.1:17845097-17845116 | MS.gene08422:CDS | 40.0% | |
| ! | ATCTAGAATCTCACAAGGAG+TGG | + | chr4.1:17844496-17844515 | MS.gene08422:CDS | 40.0% |
| ! | GCTTGTTCATTGGTAACATC+AGG | - | chr4.1:17845047-17845066 | None:intergenic | 40.0% |
| AAATTGCAACCCCTGCTAAC+TGG | + | chr4.1:17844994-17845013 | MS.gene08422:CDS | 45.0% | |
| AAGCTCAGTGGTACAAACTG+GGG | - | chr4.1:17844406-17844425 | None:intergenic | 45.0% | |
| ACCATTCTCTTCTCTCATCC+AGG | + | chr4.1:17844372-17844391 | MS.gene08422:CDS | 45.0% | |
| ACCTGGATGAGAGAAGAGAA+TGG | - | chr4.1:17844376-17844395 | None:intergenic | 45.0% | |
| CAAGCTCAGTGGTACAAACT+GGG | - | chr4.1:17844407-17844426 | None:intergenic | 45.0% | |
| GCTAAGGATATGTTCCCTCA+AGG | + | chr4.1:17845065-17845084 | MS.gene08422:CDS | 45.0% | |
| TACCACTGAGCTTGGTAAGA+TGG | + | chr4.1:17844413-17844432 | MS.gene08422:CDS | 45.0% | |
| TTCTGTACCCTGCAACAACT+GGG | + | chr4.1:17844913-17844932 | MS.gene08422:CDS | 45.0% | |
| TTTCTGTACCCTGCAACAAC+TGG | + | chr4.1:17844912-17844931 | MS.gene08422:CDS | 45.0% | |
| !! | GAACATGGATGAAGTGCTGA+GGG | + | chr4.1:17844938-17844957 | MS.gene08422:CDS | 45.0% |
| !!! | ATTGATAATATTAATTAATT+AGG | + | chr4.1:17844626-17844645 | MS.gene08422:intron | 5.0% |
| AGCCATCTTACCAAGCTCAG+TGG | - | chr4.1:17844418-17844437 | None:intergenic | 50.0% | |
| ATGTTCCTCCCAGTTGTTGC+AGG | - | chr4.1:17844924-17844943 | None:intergenic | 50.0% | |
| CAAACTGGGGTGAAATCACC+TGG | - | chr4.1:17844393-17844412 | None:intergenic | 50.0% | |
| CCAAGCTCAGTGGTACAAAC+TGG | - | chr4.1:17844408-17844427 | None:intergenic | 50.0% | |
| CCAGTTTGTACCACTGAGCT+TGG | + | chr4.1:17844405-17844424 | MS.gene08422:CDS | 50.0% | |
| GAAATGACAACAGGTTCCCC+TGG | - | chr4.1:17845023-17845042 | None:intergenic | 50.0% | |
| GGATAGTTCACCTTTGCACC+TGG | - | chr4.1:17844677-17844696 | None:intergenic | 50.0% | |
| GTAAATGCAGCCAGGTGCAA+AGG | + | chr4.1:17844664-17844683 | MS.gene08422:intron | 50.0% | |
| GTGTCACCTATAGTAAGGCC+TGG | - | chr4.1:17844282-17844301 | None:intergenic | 50.0% | |
| TGCAACAACTGGGAGGAACA+TGG | + | chr4.1:17844923-17844942 | MS.gene08422:CDS | 50.0% | |
| TGTTCCTCCCAGTTGTTGCA+GGG | - | chr4.1:17844923-17844942 | None:intergenic | 50.0% | |
| !! | GGAACATGGATGAAGTGCTG+AGG | + | chr4.1:17844937-17844956 | MS.gene08422:CDS | 50.0% |
| TGTACCCTGCAACAACTGGG+AGG | + | chr4.1:17844916-17844935 | MS.gene08422:CDS | 55.0% | |
| !! | CATGGATGAAGTGCTGAGGG+TGG | + | chr4.1:17844941-17844960 | MS.gene08422:CDS | 55.0% |
| ACCCCTGCTAACTGGAAGCC+AGG | + | chr4.1:17845002-17845021 | MS.gene08422:CDS | 60.0% | |
| CCCCTGCTAACTGGAAGCCA+GGG | + | chr4.1:17845003-17845022 | MS.gene08422:CDS | 60.0% | |
| CCCCTGGCTTCCAGTTAGCA+GGG | - | chr4.1:17845007-17845026 | None:intergenic | 60.0% | |
| CCCTGCTAACTGGAAGCCAG+GGG | + | chr4.1:17845004-17845023 | MS.gene08422:CDS | 60.0% | |
| CCCTGGCTTCCAGTTAGCAG+GGG | - | chr4.1:17845006-17845025 | None:intergenic | 60.0% | |
| TCCCCTGGCTTCCAGTTAGC+AGG | - | chr4.1:17845008-17845027 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 17844276 | 17845145 | 17844276 | ID=MS.gene08422 |
| chr4.1 | mRNA | 17844276 | 17845145 | 17844276 | ID=MS.gene08422.t1;Parent=MS.gene08422 |
| chr4.1 | exon | 17844276 | 17844542 | 17844276 | ID=MS.gene08422.t1.exon1;Parent=MS.gene08422.t1 |
| chr4.1 | CDS | 17844276 | 17844542 | 17844276 | ID=cds.MS.gene08422.t1;Parent=MS.gene08422.t1 |
| chr4.1 | exon | 17844674 | 17844817 | 17844674 | ID=MS.gene08422.t1.exon2;Parent=MS.gene08422.t1 |
| chr4.1 | CDS | 17844674 | 17844817 | 17844674 | ID=cds.MS.gene08422.t1;Parent=MS.gene08422.t1 |
| chr4.1 | exon | 17844900 | 17845145 | 17844900 | ID=MS.gene08422.t1.exon3;Parent=MS.gene08422.t1 |
| chr4.1 | CDS | 17844900 | 17845145 | 17844900 | ID=cds.MS.gene08422.t1;Parent=MS.gene08422.t1 |
| Gene Sequence |
| Protein sequence |
