AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene08462

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene08462.t1 MTR_8g023400 96.820 283 9 0 1 283 39 321 0.0 564
MS.gene08462.t1 MTR_7g117540 36.564 227 112 5 1 225 34 230 1.22e-33 124
MS.gene08462.t1 MTR_4g120270 41.935 155 63 7 6 142 36 181 2.05e-29 112
MS.gene08462.t1 MTR_2g436100 35.484 155 83 5 1 143 43 192 2.46e-24 99.8
MS.gene08462.t1 MTR_3g464780 34.868 152 80 4 12 144 47 198 1.18e-22 94.4
MS.gene08462.t1 MTR_5g064890 29.798 198 133 4 1 193 72 268 9.32e-22 93.6
MS.gene08462.t1 MTR_4g129420 38.793 116 62 3 8 121 78 186 1.27e-20 91.7
MS.gene08462.t1 MTR_5g034790 48.052 77 39 1 47 122 109 185 1.40e-20 90.5
MS.gene08462.t1 MTR_5g024730 42.574 101 54 2 54 150 77 177 1.80e-20 87.4
MS.gene08462.t1 MTR_5g071930 37.097 124 60 3 10 127 26 137 4.10e-20 86.3
MS.gene08462.t1 MTR_3g110190 52.778 72 33 1 54 124 66 137 5.60e-20 88.6
MS.gene08462.t1 MTR_5g066250 35.329 167 80 5 54 192 134 300 1.46e-19 88.2
MS.gene08462.t1 MTR_4g106680 38.636 132 66 5 47 165 99 228 1.79e-19 86.7
MS.gene08462.t1 MTR_6g087150 37.209 129 71 3 54 181 93 212 2.30e-19 87.0
MS.gene08462.t1 MTR_8g063660 48.684 76 38 1 47 121 61 136 2.58e-19 86.7
MS.gene08462.t1 MTR_4g093830 32.143 168 103 3 2 158 68 235 4.36e-19 86.3
MS.gene08462.t1 MTR_8g092840 46.835 79 39 2 54 130 113 190 6.70e-19 83.6
MS.gene08462.t1 MTR_3g116000 44.737 76 42 0 54 129 79 154 7.19e-19 82.8
MS.gene08462.t1 MTR_8g092960 46.835 79 39 2 54 130 103 180 7.53e-19 83.2
MS.gene08462.t1 MTR_1g074190 36.975 119 61 3 17 128 69 180 8.09e-19 85.5
MS.gene08462.t1 MTR_8g089880 36.792 106 56 2 53 157 126 221 1.18e-18 84.0
MS.gene08462.t1 MTR_1g100737 47.297 74 33 1 54 121 92 165 1.98e-18 81.6
MS.gene08462.t1 MTR_7g073200 35.461 141 86 3 5 143 34 171 2.42e-18 80.9
MS.gene08462.t1 MTR_3g094000 47.887 71 34 1 54 121 91 161 3.32e-18 80.5
MS.gene08462.t1 MTR_8g093010 41.935 93 50 3 40 130 94 184 4.94e-18 80.9
MS.gene08462.t1 MTR_3g100400 48.000 75 38 1 54 127 130 204 6.42e-18 83.2
MS.gene08462.t1 MTR_1g043410 42.157 102 51 4 33 126 69 170 6.96e-18 82.4
MS.gene08462.t1 MTR_7g113800 43.678 87 42 3 45 124 80 166 7.67e-18 82.8
MS.gene08462.t1 MTR_6g007737 48.750 80 32 2 47 121 126 201 8.26e-18 82.8
MS.gene08462.t1 MTR_2g096350 42.308 104 48 3 24 124 86 180 1.26e-17 80.1
MS.gene08462.t1 MTR_4g063070 37.600 125 64 5 4 121 261 378 1.37e-17 82.4
MS.gene08462.t1 MTR_8g092930 35.652 115 67 3 54 166 112 221 1.60e-17 79.7
MS.gene08462.t1 MTR_8g092985 44.304 79 41 2 54 130 111 188 1.99e-17 79.3
MS.gene08462.t1 MTR_8g073120 41.250 80 44 1 54 130 70 149 2.13e-17 79.0
MS.gene08462.t1 MTR_3g091620 40.230 87 46 2 43 128 95 176 2.33e-17 81.3
MS.gene08462.t1 MTR_1g082550 47.368 76 35 2 54 124 98 173 4.16e-17 80.9
MS.gene08462.t1 MTR_3g095560 46.154 78 39 2 54 129 109 185 4.43e-17 79.0
MS.gene08462.t1 MTR_5g030010 48.611 72 36 1 54 124 122 193 6.08e-17 80.1
MS.gene08462.t1 MTR_2g090950 46.154 78 35 1 54 124 91 168 6.78e-17 78.2
MS.gene08462.t1 MTR_6g055030 46.914 81 39 2 54 130 97 177 7.93e-17 79.3
MS.gene08462.t1 MTR_7g076250 41.463 82 38 2 42 121 59 132 9.85e-17 77.0
MS.gene08462.t1 MTR_2g009310 43.038 79 44 1 44 121 125 203 1.06e-16 79.7
MS.gene08462.t1 MTR_2g090905 44.156 77 36 1 55 124 40 116 1.43e-16 74.7
MS.gene08462.t1 MTR_5g064990 37.600 125 68 4 4 121 64 185 1.57e-16 79.3
MS.gene08462.t1 MTR_7g108855 37.143 140 68 5 32 167 47 170 2.00e-16 76.3
MS.gene08462.t1 MTR_5g082940 31.858 113 62 2 18 127 51 151 2.98e-16 75.1
MS.gene08462.t1 MTR_4g030070 31.973 147 88 5 53 191 84 226 3.51e-16 76.3
MS.gene08462.t1 MTR_3g099650 36.719 128 70 6 4 124 59 182 3.91e-16 77.8
MS.gene08462.t1 MTR_1g090480 33.071 127 74 3 1 122 25 145 4.16e-16 74.7
MS.gene08462.t1 MTR_3g086730 40.952 105 50 3 58 150 50 154 9.21e-16 75.1
MS.gene08462.t1 MTR_1g070140 41.892 74 41 1 54 125 103 176 2.79e-15 75.1
MS.gene08462.t1 MTR_8g092970 40.244 82 44 2 78 159 130 206 2.99e-15 73.6
MS.gene08462.t1 MTR_2g055790 47.059 68 34 1 54 121 77 142 3.27e-15 72.8
MS.gene08462.t1 MTR_8g092990 52.830 53 24 1 78 130 140 191 3.94e-15 73.2
MS.gene08462.t1 MTR_3g086700 50.725 69 30 2 58 122 50 118 4.79e-15 72.0
MS.gene08462.t1 MTR_4g094725 42.105 76 41 1 54 126 78 153 8.93e-15 70.5
MS.gene08462.t1 MTR_5g026730 59.574 47 19 0 75 121 90 136 2.21e-14 70.1
MS.gene08462.t1 MTR_3g012550 38.961 77 44 1 49 122 75 151 2.42e-14 70.1
MS.gene08462.t1 MTR_5g064980 44.444 72 36 2 54 121 108 179 2.95e-14 72.4
MS.gene08462.t1 MTR_3g114500 48.529 68 33 1 54 121 48 113 3.31e-14 68.2
MS.gene08462.t1 MTR_8g066780 46.667 75 37 2 53 124 113 187 3.86e-14 72.0
MS.gene08462.t1 MTR_3g071300 39.286 84 46 2 54 132 76 159 4.52e-14 69.3
MS.gene08462.t1 MTR_1g492830 35.106 94 59 1 30 121 37 130 5.32e-14 70.1
MS.gene08462.t1 MTR_8g066740 38.710 93 45 3 54 139 76 163 7.85e-14 70.9
MS.gene08462.t1 MTR_1g026070 40.244 82 47 1 54 135 48 127 8.73e-14 67.4
MS.gene08462.t1 MTR_7g116220 38.667 75 38 2 54 122 70 142 1.02e-13 68.2
MS.gene08462.t1 MTR_2g035800 42.466 73 39 2 54 124 76 147 1.20e-13 67.8
MS.gene08462.t1 MTR_5g026720 56.250 48 21 0 75 122 89 136 1.23e-13 67.8
MS.gene08462.t1 MTR_4g056590 41.096 73 41 2 54 126 100 170 2.08e-13 67.8
MS.gene08462.t1 MTR_6g007680 39.024 82 49 1 60 140 46 127 2.51e-13 66.6
MS.gene08462.t1 MTR_8g071815 45.455 66 32 1 77 138 88 153 2.27e-12 64.7
MS.gene08462.t1 MTR_1g090460 44.776 67 32 1 62 123 73 139 2.39e-12 63.9
MS.gene08462.t1 MTR_7g079280 35.616 73 44 1 53 122 79 151 3.32e-12 63.9
MS.gene08462.t1 MTR_7g116180 37.838 74 40 2 54 122 67 139 3.49e-12 63.9
MS.gene08462.t1 MTR_3g078450 36.000 75 45 1 51 122 73 147 3.54e-12 64.7
MS.gene08462.t1 MTR_5g076990 36.264 91 50 3 54 139 76 163 3.61e-12 65.9
MS.gene08462.t1 MTR_7g096690 35.165 91 51 3 43 125 56 146 4.36e-12 65.9
MS.gene08462.t1 MTR_7g116200 36.232 69 44 0 54 122 68 136 5.30e-12 63.5
MS.gene08462.t1 MTR_2g041200 58.333 48 20 0 78 125 108 155 5.78e-12 63.5
MS.gene08462.t1 MTR_1g070425 37.838 74 40 2 54 122 68 140 5.89e-12 63.5
MS.gene08462.t1 MTR_8g099495 51.111 45 22 0 79 123 347 391 6.41e-12 65.9
MS.gene08462.t1 MTR_7g079290 35.897 78 41 2 54 124 125 200 7.05e-12 63.9
MS.gene08462.t1 MTR_5g022920 57.778 45 19 0 77 121 187 231 7.60e-12 63.9
MS.gene08462.t1 MTR_6g007670 30.769 104 65 1 60 163 14 110 8.01e-12 64.3
MS.gene08462.t1 MTR_8g028465 37.143 70 43 1 53 122 89 157 9.28e-12 63.2
MS.gene08462.t1 MTR_7g059360 45.902 61 31 1 67 125 144 204 9.32e-12 63.9
MS.gene08462.t1 MTR_6g055030 56.000 50 22 0 81 130 97 146 9.87e-12 64.3
MS.gene08462.t1 MTR_8g032690 53.488 43 20 0 79 121 19 61 1.38e-11 60.5
MS.gene08462.t1 MTR_4g108830 42.857 70 38 2 54 122 149 217 1.88e-11 62.8
MS.gene08462.t1 MTR_4g108830 42.857 70 38 2 54 122 121 189 1.97e-11 62.0
MS.gene08462.t1 MTR_3g081030 44.444 72 36 3 52 121 92 161 2.03e-11 62.8
MS.gene08462.t1 MTR_3g078470 36.000 75 45 2 51 122 73 147 2.22e-11 62.4
MS.gene08462.t1 MTR_4g022410 52.000 50 24 0 76 125 110 159 2.38e-11 62.0
MS.gene08462.t1 MTR_7g116210 35.294 68 44 0 54 121 10 77 4.33e-11 59.3
MS.gene08462.t1 MTR_1g096780 42.254 71 37 3 54 121 208 277 4.41e-11 63.2
MS.gene08462.t1 MTR_5g009210 53.333 45 21 0 79 123 315 359 5.77e-11 62.8
MS.gene08462.t1 MTR_5g009200 53.333 45 21 0 79 123 350 394 6.47e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene08462.t1 AT1G53820 46.209 277 110 10 12 266 45 304 9.18e-62 197
MS.gene08462.t1 AT1G72310 39.310 290 132 6 1 267 34 302 1.58e-57 187
MS.gene08462.t1 AT3G16720 35.252 278 132 12 1 273 40 274 1.52e-32 122
MS.gene08462.t1 AT4G15975 42.241 116 55 3 9 124 18 121 4.02e-24 97.8
MS.gene08462.t1 AT3G14320 47.368 76 40 0 52 127 61 136 2.45e-22 92.4
MS.gene08462.t1 AT1G49220 47.170 106 45 4 32 130 84 185 4.00e-22 92.8
MS.gene08462.t1 AT5G05280 48.810 84 42 1 39 121 72 155 4.53e-22 90.9
MS.gene08462.t1 AT5G17600 46.667 105 51 3 54 157 116 216 4.86e-22 94.7
MS.gene08462.t1 AT1G23980 36.000 125 75 3 2 121 62 186 1.16e-20 90.9
MS.gene08462.t1 AT2G20030 52.000 75 35 1 54 127 98 172 1.34e-20 90.9
MS.gene08462.t1 AT3G18773 41.584 101 56 2 54 152 103 202 1.44e-20 88.2
MS.gene08462.t1 AT1G04360 41.667 108 49 4 32 128 79 183 2.59e-20 90.1
MS.gene08462.t1 AT1G49210 50.633 79 36 2 54 130 108 185 7.47e-20 86.3
MS.gene08462.t1 AT1G49200 48.810 84 40 2 49 130 104 186 1.00e-19 85.9
MS.gene08462.t1 AT1G20823 44.944 89 45 2 43 129 75 161 1.87e-19 84.3
MS.gene08462.t1 AT3G62690 36.800 125 74 2 1 124 38 158 2.14e-19 85.5
MS.gene08462.t1 AT3G48030 33.333 138 88 2 1 136 51 186 2.23e-19 85.9
MS.gene08462.t1 AT5G05810 52.000 75 35 1 54 127 120 194 2.49e-19 87.4
MS.gene08462.t1 AT4G40070 44.444 90 46 2 50 135 91 180 2.64e-19 86.7
MS.gene08462.t1 AT3G10910 44.565 92 48 2 54 143 88 178 3.96e-19 83.2
MS.gene08462.t1 AT3G48030 33.333 138 88 2 1 136 129 264 6.83e-19 85.5
MS.gene08462.t1 AT1G76410 42.529 87 47 2 45 129 69 154 7.02e-19 82.4
MS.gene08462.t1 AT4G28890 44.792 96 50 2 54 148 97 190 7.59e-19 86.3
MS.gene08462.t1 AT4G30400 42.157 102 52 3 25 121 77 176 1.49e-18 85.5
MS.gene08462.t1 AT5G43420 35.570 149 72 5 32 161 78 221 2.45e-18 84.3
MS.gene08462.t1 AT5G10380 52.857 70 32 1 53 121 108 177 5.65e-18 82.4
MS.gene08462.t1 AT2G42360 36.000 125 65 2 2 124 42 153 6.40e-18 81.3
MS.gene08462.t1 AT2G35000 33.505 194 117 7 1 184 52 243 8.08e-18 82.8
MS.gene08462.t1 AT3G03550 38.182 110 55 2 55 151 134 243 8.11e-18 82.8
MS.gene08462.t1 AT2G18650 45.977 87 44 3 42 125 87 173 1.65e-17 82.4
MS.gene08462.t1 AT3G19140 41.176 85 44 1 43 121 37 121 1.90e-17 77.4
MS.gene08462.t1 AT5G01880 45.833 72 38 1 52 122 77 148 2.38e-17 77.8
MS.gene08462.t1 AT3G05200 37.500 104 56 3 54 148 99 202 2.83e-17 81.6
MS.gene08462.t1 AT4G10150 39.080 87 51 1 35 121 69 153 4.13e-17 79.0
MS.gene08462.t1 AT1G33480 40.698 86 48 1 36 121 60 142 4.56e-17 79.3
MS.gene08462.t1 AT5G40250 45.570 79 32 1 54 121 107 185 4.85e-17 80.5
MS.gene08462.t1 AT4G33565 36.792 106 66 1 44 148 181 286 6.05e-17 80.1
MS.gene08462.t1 AT4G10160 41.667 72 42 0 50 121 68 139 1.03e-16 77.8
MS.gene08462.t1 AT2G17450 40.777 103 48 3 35 129 55 152 1.08e-16 76.6
MS.gene08462.t1 AT4G17905 44.186 86 45 2 44 126 117 202 1.51e-16 78.6
MS.gene08462.t1 AT4G17905 42.222 90 49 2 44 130 143 232 1.79e-16 78.6
MS.gene08462.t1 AT1G49230 40.659 91 51 2 54 142 104 193 2.20e-16 76.6
MS.gene08462.t1 AT4G09120 38.144 97 55 2 35 127 76 171 2.62e-16 78.2
MS.gene08462.t1 AT2G27940 41.096 73 43 0 50 122 111 183 4.09e-16 76.3
MS.gene08462.t1 AT2G42350 40.230 87 52 0 35 121 56 142 4.71e-16 75.5
MS.gene08462.t1 AT5G27420 41.463 82 44 2 54 131 95 176 5.09e-16 77.8
MS.gene08462.t1 AT2G47560 36.220 127 67 3 1 124 38 153 6.42e-16 75.5
MS.gene08462.t1 AT1G72200 29.944 177 95 5 3 155 62 233 7.79e-16 77.4
MS.gene08462.t1 AT4G09110 38.144 97 55 2 35 127 76 171 7.84e-16 76.3
MS.gene08462.t1 AT1G32361 36.364 99 56 3 29 127 111 202 8.16e-16 76.6
MS.gene08462.t1 AT2G34990 43.056 72 37 1 54 121 89 160 9.18e-16 76.6
MS.gene08462.t1 AT5G42200 35.593 118 59 3 5 121 45 146 9.19e-16 73.6
MS.gene08462.t1 AT2G34990 43.056 72 37 1 54 121 67 138 1.09e-15 75.9
MS.gene08462.t1 AT1G72220 44.595 74 40 1 54 126 151 224 1.18e-15 77.0
MS.gene08462.t1 AT4G09130 37.363 91 53 2 42 128 79 169 1.64e-15 76.3
MS.gene08462.t1 AT2G35420 32.520 123 73 4 2 121 30 145 2.34e-15 74.3
MS.gene08462.t1 AT1G74410 42.500 80 44 2 47 124 142 221 2.59e-15 73.6
MS.gene08462.t1 AT4G17920 39.394 99 55 3 54 148 81 178 4.74e-15 73.9
MS.gene08462.t1 AT5G07040 39.189 74 42 1 52 122 64 137 7.40e-15 70.9
MS.gene08462.t1 AT3G18930 36.559 93 47 2 44 124 109 201 1.31e-14 73.9
MS.gene08462.t1 AT3G18930 36.559 93 47 2 44 124 109 201 1.31e-14 73.9
MS.gene08462.t1 AT3G18930 36.559 93 47 2 44 124 109 201 1.31e-14 73.9
MS.gene08462.t1 AT5G57750 40.000 80 40 2 48 122 89 165 1.51e-14 71.2
MS.gene08462.t1 AT1G22500 40.217 92 50 2 40 126 75 166 2.06e-14 73.2
MS.gene08462.t1 AT5G58580 39.286 112 60 3 54 157 111 222 2.58e-14 72.0
MS.gene08462.t1 AT4G09100 44.000 75 38 1 54 124 56 130 3.53e-14 68.6
MS.gene08462.t1 AT1G35330 41.667 72 38 1 54 121 100 171 9.39e-14 70.9
MS.gene08462.t1 AT3G61550 31.034 116 60 4 20 122 71 179 3.35e-13 67.8
MS.gene08462.t1 AT4G24015 39.773 88 51 2 53 139 79 165 9.43e-13 65.5
MS.gene08462.t1 AT5G46650 47.297 74 32 3 54 121 85 157 1.06e-12 67.4
MS.gene08462.t1 AT3G60220 38.372 86 46 2 44 122 75 160 1.54e-12 67.4
MS.gene08462.t1 AT2G46493 45.833 48 26 0 75 122 134 181 1.84e-12 65.1
MS.gene08462.t1 AT4G35480 38.889 72 44 0 72 143 106 177 2.18e-12 65.1
MS.gene08462.t1 AT2G46160 30.612 98 57 2 36 122 84 181 2.60e-12 65.1
MS.gene08462.t1 AT2G46160 30.612 98 57 2 36 122 84 181 2.60e-12 65.1
MS.gene08462.t1 AT1G71980 36.782 87 51 3 54 137 205 290 3.34e-12 66.6
MS.gene08462.t1 AT5G06490 34.615 78 43 1 53 122 95 172 4.63e-12 64.3
MS.gene08462.t1 AT2G44578 58.140 43 18 0 79 121 70 112 6.36e-12 62.4
MS.gene08462.t1 AT2G46494 47.917 48 25 0 75 122 312 359 7.07e-12 65.5
MS.gene08462.t1 AT5G53110 36.735 98 54 3 33 122 276 373 8.06e-12 65.5
MS.gene08462.t1 AT5G66070 55.556 45 20 0 77 121 174 218 1.45e-11 63.2
MS.gene08462.t1 AT5G66070 55.556 45 20 0 77 121 174 218 1.45e-11 63.2
MS.gene08462.t1 AT5G47610 41.270 63 35 1 64 124 90 152 1.88e-11 61.6
MS.gene08462.t1 AT2G17730 40.278 72 39 1 54 121 167 238 2.01e-11 63.2
MS.gene08462.t1 AT2G34000 33.333 99 58 2 33 123 37 135 2.11e-11 61.2
MS.gene08462.t1 AT5G66070 55.556 45 20 0 77 121 198 242 2.18e-11 63.2
MS.gene08462.t1 AT1G28040 45.833 48 26 0 75 122 249 296 2.90e-11 63.2
MS.gene08462.t1 AT1G22670 35.632 87 52 3 54 137 205 290 6.23e-11 62.8
MS.gene08462.t1 AT2G44581 53.488 43 20 0 79 121 70 112 8.22e-11 59.3
MS.gene08462.t1 AT3G60966 60.465 43 17 0 79 121 62 104 8.86e-11 59.3
MS.gene08462.t1 AT2G46495 45.833 48 26 0 75 122 316 363 9.14e-11 62.0
MS.gene08462.t1 AT2G46495 45.833 48 26 0 75 122 288 335 9.30e-11 62.0

Find 49 sgRNAs with CRISPR-Local

Find 61 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTCAAGATCCTGTTGTTTA+TGG 0.168039 8.2:+72467784 MS.gene08462:CDS
CCATTTGAATCAGTACTATT+TGG 0.192512 8.2:-72468051 None:intergenic
TCTTAAGTCTTTGCCTGTTT+TGG 0.218884 8.2:+72467837 MS.gene08462:CDS
TGAAACCCATGATTGCATTT+TGG 0.226368 8.2:-72467946 None:intergenic
CGTCGACGACGGCGGTTTCT+TGG 0.237232 8.2:-72467742 None:intergenic
ATTCTACTTGTCCTCTTTGT+AGG 0.257676 8.2:+72468001 MS.gene08462:CDS
ATTTATATGCAAAGTGGTTT+TGG 0.278266 8.2:+72467689 MS.gene08462:CDS
CATTGTCACCTTCTGATAAT+AGG 0.288696 8.2:+72468361 MS.gene08462:CDS
TCCTTCAACTCCAAGTTCTA+TGG 0.291895 8.2:+72468467 MS.gene08462:CDS
AAATTTCCCTACAAATGTTT+TGG 0.305638 8.2:+72468143 MS.gene08462:CDS
GTTTATGGAAGTCACCAAAT+TGG 0.322819 8.2:+72467799 MS.gene08462:CDS
ACACAAGTTAGTTCTTCTTT+TGG 0.347982 8.2:+72468183 MS.gene08462:CDS
TGAGGATTTCAAAGATGGTT+TGG 0.348325 8.2:+72467870 MS.gene08462:CDS
AGTAATAGATGTAATGGAAA+TGG 0.371817 8.2:+72468291 MS.gene08462:CDS
TCATCATCATCATCTACTTT+AGG 0.382831 8.2:+72468252 MS.gene08462:CDS
CCCCAAACCAAAACATTTGT+AGG 0.395435 8.2:-72468150 None:intergenic
AAGAATGAGGATTTCAAAGA+TGG 0.398458 8.2:+72467865 MS.gene08462:CDS
CAAGATTGAGGTCATTTAAG+AGG 0.400414 8.2:+72468418 MS.gene08462:CDS
TCTACTTTAGGTAGTGATAA+TGG 0.408867 8.2:+72468264 MS.gene08462:CDS
GGTAATAGTAATAGATGTAA+TGG 0.421867 8.2:+72468285 MS.gene08462:CDS
TGTCTTTGTGATGTTGTTGA+AGG 0.438454 8.2:+72467904 MS.gene08462:CDS
TCATTCTTGAAAACCAAAAC+AGG 0.442504 8.2:-72467850 None:intergenic
TCCATAGAACTTGGAGTTGA+AGG 0.444903 8.2:-72468468 None:intergenic
ATTTAGATTGTATTGATATG+TGG 0.449698 8.2:+72467968 MS.gene08462:CDS
TGGTGACTTCCATAAACAAC+AGG 0.454316 8.2:-72467793 None:intergenic
CGACGGCGGTTTCTTGGTTG+AGG 0.455007 8.2:-72467736 None:intergenic
GACCTCAATCTTGTTGTCAT+TGG 0.460150 8.2:-72468408 None:intergenic
TTAGAAGTCACTACTAGCTC+TGG 0.474659 8.2:-72468503 None:intergenic
TGTTCCACATCCATAGAACT+TGG 0.475183 8.2:-72468477 None:intergenic
AACTCCAAGTTCTATGGATG+TGG 0.479911 8.2:+72468473 MS.gene08462:CDS
GAACTAAATTCACTTGGTAA+TGG 0.506647 8.2:-72468333 None:intergenic
CCAAATAGTACTGATTCAAA+TGG 0.506743 8.2:+72468051 MS.gene08462:CDS
GAAAATTTGAGTCATGTGAA+TGG 0.511787 8.2:+72468108 MS.gene08462:CDS
AAACAAACCGCCGTCGACGA+CGG 0.534947 8.2:-72467753 None:intergenic
CCCAAACCAAAACATTTGTA+GGG 0.536138 8.2:-72468149 None:intergenic
TGCTTCATTTATATGCAAAG+TGG 0.539115 8.2:+72467683 MS.gene08462:CDS
CACCAATGACAACAAGATTG+AGG 0.562761 8.2:+72468406 MS.gene08462:CDS
GAAACTAAATTCCTACAAAG+AGG 0.564972 8.2:-72468012 None:intergenic
CAAGAAACCGCCGTCGTCGA+CGG 0.576823 8.2:+72467743 MS.gene08462:CDS
AGTTCTATGGATGTGGAACA+AGG 0.581073 8.2:+72468480 MS.gene08462:CDS
ATGTAATGGAAATGGAATGT+TGG 0.589041 8.2:+72468299 MS.gene08462:CDS
GAGGAAGAACTAAATTCACT+TGG 0.635097 8.2:-72468339 None:intergenic
TCAACACACCTATTATCAGA+AGG 0.647870 8.2:-72468369 None:intergenic
AATGTGTTAGTCTCATCAGA+AGG 0.648849 8.2:+72468084 MS.gene08462:CDS
CAAACCGCCGTCGACGACGG+CGG 0.650391 8.2:-72467750 None:intergenic
GAAACCGCCGTCGTCGACGG+CGG 0.652134 8.2:+72467746 MS.gene08462:CDS
ATTATCAGAAGGTGACAATG+AGG 0.655023 8.2:-72468358 None:intergenic
TAAACAACAGGATCTTGACC+AGG 0.667684 8.2:-72467781 None:intergenic
GATGATGATGATGCTGAACA+AGG 0.671827 8.2:-72468240 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTAGATTGTATTGATATG+TGG + chr8.2:72467968-72467987 MS.gene08462:CDS 20.0%
! GGTAATAGTAATAGATGTAA+TGG + chr8.2:72468285-72468304 MS.gene08462:CDS 25.0%
!! AAATTTCCCTACAAATGTTT+TGG + chr8.2:72468143-72468162 MS.gene08462:CDS 25.0%
!! AGTAATAGATGTAATGGAAA+TGG + chr8.2:72468291-72468310 MS.gene08462:CDS 25.0%
!! ATTTATATGCAAAGTGGTTT+TGG + chr8.2:72467689-72467708 MS.gene08462:CDS 25.0%
!! TTTTACCAAAATGCAATCAT+GGG + chr8.2:72467941-72467960 MS.gene08462:CDS 25.0%
AAGAATGAGGATTTCAAAGA+TGG + chr8.2:72467865-72467884 MS.gene08462:CDS 30.0%
CCATTTGAATCAGTACTATT+TGG - chr8.2:72468054-72468073 None:intergenic 30.0%
GAAAATTTGAGTCATGTGAA+TGG + chr8.2:72468108-72468127 MS.gene08462:CDS 30.0%
GAAACTAAATTCCTACAAAG+AGG - chr8.2:72468015-72468034 None:intergenic 30.0%
GAACTAAATTCACTTGGTAA+TGG - chr8.2:72468336-72468355 None:intergenic 30.0%
TCATCATCATCATCTACTTT+AGG + chr8.2:72468252-72468271 MS.gene08462:CDS 30.0%
TCATTCTTGAAAACCAAAAC+AGG - chr8.2:72467853-72467872 None:intergenic 30.0%
TCTACTTTAGGTAGTGATAA+TGG + chr8.2:72468264-72468283 MS.gene08462:CDS 30.0%
TGCTTCATTTATATGCAAAG+TGG + chr8.2:72467683-72467702 MS.gene08462:CDS 30.0%
TTGTTGAAGGAGAAAAAACT+AGG + chr8.2:72467917-72467936 MS.gene08462:CDS 30.0%
! ATGTAATGGAAATGGAATGT+TGG + chr8.2:72468299-72468318 MS.gene08462:CDS 30.0%
! CTTTTACCAAAATGCAATCA+TGG + chr8.2:72467940-72467959 MS.gene08462:CDS 30.0%
!! ACACAAGTTAGTTCTTCTTT+TGG + chr8.2:72468183-72468202 MS.gene08462:CDS 30.0%
!! CCAAATAGTACTGATTCAAA+TGG + chr8.2:72468051-72468070 MS.gene08462:CDS 30.0%
!!! TGTTTTGGTTTTCAAGAATG+AGG + chr8.2:72467852-72467871 MS.gene08462:CDS 30.0%
!!! TTCTTCTTTTGGTGTTTCTT+TGG + chr8.2:72468194-72468213 MS.gene08462:CDS 30.0%
!!! TTTTAGCTTCAAGTCCAATT+TGG - chr8.2:72467816-72467835 None:intergenic 30.0%
ATTATCAGAAGGTGACAATG+AGG - chr8.2:72468361-72468380 None:intergenic 35.0%
ATTCTACTTGTCCTCTTTGT+AGG + chr8.2:72468001-72468020 MS.gene08462:CDS 35.0%
CAAGATTGAGGTCATTTAAG+AGG + chr8.2:72468418-72468437 MS.gene08462:CDS 35.0%
CATTGTCACCTTCTGATAAT+AGG + chr8.2:72468361-72468380 MS.gene08462:CDS 35.0%
CCCAAACCAAAACATTTGTA+GGG - chr8.2:72468152-72468171 None:intergenic 35.0%
GAGGAAGAACTAAATTCACT+TGG - chr8.2:72468342-72468361 None:intergenic 35.0%
GTTTATGGAAGTCACCAAAT+TGG + chr8.2:72467799-72467818 MS.gene08462:CDS 35.0%
TCAACACACCTATTATCAGA+AGG - chr8.2:72468372-72468391 None:intergenic 35.0%
TGAGGATTTCAAAGATGGTT+TGG + chr8.2:72467870-72467889 MS.gene08462:CDS 35.0%
TGTCTTTGTGATGTTGTTGA+AGG + chr8.2:72467904-72467923 MS.gene08462:CDS 35.0%
! AATGTGTTAGTCTCATCAGA+AGG + chr8.2:72468084-72468103 MS.gene08462:CDS 35.0%
! TCTTAAGTCTTTGCCTGTTT+TGG + chr8.2:72467837-72467856 MS.gene08462:CDS 35.0%
! TGAAACCCATGATTGCATTT+TGG - chr8.2:72467949-72467968 None:intergenic 35.0%
!!! CCCTACAAATGTTTTGGTTT+GGG + chr8.2:72468149-72468168 MS.gene08462:CDS 35.0%
!!! CCTACAAATGTTTTGGTTTG+GGG + chr8.2:72468150-72468169 MS.gene08462:CDS 35.0%
!!! CTACAAATGTTTTGGTTTGG+GGG + chr8.2:72468151-72468170 MS.gene08462:CDS 35.0%
!!! TATATGCAAAGTGGTTTTGG+TGG + chr8.2:72467692-72467711 MS.gene08462:CDS 35.0%
!!! TCCCTACAAATGTTTTGGTT+TGG + chr8.2:72468148-72468167 MS.gene08462:CDS 35.0%
AACTCCAAGTTCTATGGATG+TGG + chr8.2:72468473-72468492 MS.gene08462:CDS 40.0%
AGTTCTATGGATGTGGAACA+AGG + chr8.2:72468480-72468499 MS.gene08462:CDS 40.0%
CACCAATGACAACAAGATTG+AGG + chr8.2:72468406-72468425 MS.gene08462:CDS 40.0%
CCCCAAACCAAAACATTTGT+AGG - chr8.2:72468153-72468172 None:intergenic 40.0%
TAAACAACAGGATCTTGACC+AGG - chr8.2:72467784-72467803 None:intergenic 40.0%
TCCATAGAACTTGGAGTTGA+AGG - chr8.2:72468471-72468490 None:intergenic 40.0%
TCCTTCAACTCCAAGTTCTA+TGG + chr8.2:72468467-72468486 MS.gene08462:CDS 40.0%
TGGTGACTTCCATAAACAAC+AGG - chr8.2:72467796-72467815 None:intergenic 40.0%
TGTTCCACATCCATAGAACT+TGG - chr8.2:72468480-72468499 None:intergenic 40.0%
! GACCTCAATCTTGTTGTCAT+TGG - chr8.2:72468411-72468430 None:intergenic 40.0%
! GATGATGATGATGCTGAACA+AGG - chr8.2:72468243-72468262 None:intergenic 40.0%
! GGTCAAGATCCTGTTGTTTA+TGG + chr8.2:72467784-72467803 MS.gene08462:CDS 40.0%
!!! GTGGTTTTGGTGGCGTTTTG+AGG + chr8.2:72467702-72467721 MS.gene08462:CDS 50.0%
AAACAAACCGCCGTCGACGA+CGG - chr8.2:72467756-72467775 None:intergenic 55.0%
!!! CGGCGGTTTGTTTTTGCTCC+TGG + chr8.2:72467763-72467782 MS.gene08462:CDS 55.0%
CAAGAAACCGCCGTCGTCGA+CGG + chr8.2:72467743-72467762 MS.gene08462:CDS 60.0%
CGACGGCGGTTTCTTGGTTG+AGG - chr8.2:72467739-72467758 None:intergenic 60.0%
CGTCGACGACGGCGGTTTCT+TGG - chr8.2:72467745-72467764 None:intergenic 65.0%
CAAACCGCCGTCGACGACGG+CGG - chr8.2:72467753-72467772 None:intergenic 70.0%
GAAACCGCCGTCGTCGACGG+CGG + chr8.2:72467746-72467765 MS.gene08462:CDS 70.0%


Chromosome Type Strat End Strand Name
chr8.2 gene 72467661 72468512 72467661 ID=MS.gene08462
chr8.2 mRNA 72467661 72468512 72467661 ID=MS.gene08462.t1;Parent=MS.gene08462
chr8.2 exon 72467661 72468512 72467661 ID=MS.gene08462.t1.exon1;Parent=MS.gene08462.t1
chr8.2 CDS 72467661 72468512 72467661 ID=cds.MS.gene08462.t1;Parent=MS.gene08462.t1
Gene Sequence

>MS.gene08462

ATGGTTGTTATGTTTGTTCTAATGCTTCATTTATATGCAAAGTGGTTTTGGTGGCGTTTTGAGGAAAGAACTGTTCCTCAACCAAGAAACCGCCGTCGTCGACGGCGGTTTGTTTTTGCTCCTGGTCAAGATCCTGTTGTTTATGGAAGTCACCAAATTGGACTTGAAGCTAAAATTCTTAAGTCTTTGCCTGTTTTGGTTTTCAAGAATGAGGATTTCAAAGATGGTTTGGAATGTGCTGTTTGTCTTTGTGATGTTGTTGAAGGAGAAAAAACTAGGCTTTTACCAAAATGCAATCATGGGTTTCATTTAGATTGTATTGATATGTGGTTTCAATCTCATTCTACTTGTCCTCTTTGTAGGAATTTAGTTTCTGTTGAATCATGTAAACCAAATAGTACTGATTCAAATGGTGAAGAGATGAATGTGTTAGTCTCATCAGAAGGTGAAAATTTGAGTCATGTGAATGGTTTAGAGTCTTCAAATTTCCCTACAAATGTTTTGGTTTGGGGGAATTCTCAAACACAAGTTAGTTCTTCTTTTGGTGTTTCTTTGGAAGAAAGAAGTTCTCATCAACAACCTTGTTCAGCATCATCATCATCATCTACTTTAGGTAGTGATAATGGTAATAGTAATAGATGTAATGGAAATGGAATGTTGGTGATTGATATACCATTACCAAGTGAATTTAGTTCTTCCTCATTGTCACCTTCTGATAATAGGTGTGTTGAAGATGAGATGAAATCACCAATGACAACAAGATTGAGGTCATTTAAGAGGCTTTTAAGTAGAGACAAAAAGTTTAGTCCTTCAACTCCAAGTTCTATGGATGTGGAACAAGGCCAGAGCTAG
Protein sequence

>MS.gene08462.t1

MVVMFVLMLHLYAKWFWWRFEERTVPQPRNRRRRRRFVFAPGQDPVVYGSHQIGLEAKILKSLPVLVFKNEDFKDGLECAVCLCDVVEGEKTRLLPKCNHGFHLDCIDMWFQSHSTCPLCRNLVSVESCKPNSTDSNGEEMNVLVSSEGENLSHVNGLESSNFPTNVLVWGNSQTQVSSSFGVSLEERSSHQQPCSASSSSSTLGSDNGNSNRCNGNGMLVIDIPLPSEFSSSSLSPSDNRCVEDEMKSPMTTRLRSFKRLLSRDKKFSPSTPSSMDVEQGQS