Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09018.t1 | RHN42498.1 | 95.3 | 127 | 6 | 0 | 1 | 127 | 82 | 208 | 7.30E-48 | 199.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09018.t1 | G7LH97 | 96.0 | 126 | 5 | 0 | 2 | 127 | 1 | 126 | 5.3e-48 | 199.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050463 | MS.gene09018 | 0.857298 | 1.79E-62 | -1.69E-46 |
| MS.gene050528 | MS.gene09018 | 0.854316 | 1.34E-61 | -1.69E-46 |
| MS.gene051946 | MS.gene09018 | 0.814177 | 1.76E-51 | -1.69E-46 |
| MS.gene057014 | MS.gene09018 | 0.801163 | 1.02E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09018.t1 | MTR_8g097040 | 94.574 | 129 | 7 | 0 | 2 | 130 | 1 | 129 | 1.02e-84 | 243 |
| MS.gene09018.t1 | MTR_3g084990 | 55.147 | 136 | 47 | 4 | 1 | 130 | 1 | 128 | 2.77e-42 | 136 |
| MS.gene09018.t1 | MTR_5g078400 | 54.054 | 74 | 27 | 2 | 17 | 90 | 5 | 71 | 1.23e-16 | 69.7 |
| MS.gene09018.t1 | MTR_7g028805 | 42.308 | 130 | 60 | 3 | 1 | 117 | 1 | 128 | 1.30e-16 | 71.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09018.t1 | AT1G27290 | 51.825 | 137 | 57 | 4 | 1 | 130 | 1 | 135 | 8.83e-38 | 125 |
| MS.gene09018.t1 | AT1G27290 | 51.825 | 137 | 57 | 4 | 1 | 130 | 1 | 135 | 8.83e-38 | 125 |
Find 34 sgRNAs with CRISPR-Local
Find 35 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGATCGGTTACTTTCGTGAT+TGG | 0.305883 | 4.4:+15280626 | MS.gene09018:CDS |
| ATGTTGATGCCGTGGGTTAT+TGG | 0.333475 | 4.4:+15280653 | MS.gene09018:CDS |
| CATAGATTATGATTTCTTAC+CGG | 0.352525 | 4.4:-15280771 | None:intergenic |
| GTGGGTTATTGGATTGGTTA+TGG | 0.372418 | 4.4:+15280664 | MS.gene09018:CDS |
| GGATTTGCGTCGTTTGTTCT+CGG | 0.413462 | 4.4:+15280578 | MS.gene09018:CDS |
| GATTTGCGTCGTTTGTTCTC+GGG | 0.415303 | 4.4:+15280579 | MS.gene09018:CDS |
| GTCGTTTGTTCTCGGGATTT+CGG | 0.429794 | 4.4:+15280586 | MS.gene09018:CDS |
| GATCGGTTACTTTCGTGATT+GGG | 0.431361 | 4.4:+15280627 | MS.gene09018:CDS |
| TTGAGACGTTCGTCGGCGGC+AGG | 0.478548 | 4.4:+15280539 | MS.gene09018:CDS |
| GGCTTTGATGCTGTGTGGAT+CGG | 0.501281 | 4.4:+15280610 | MS.gene09018:CDS |
| GATGCCGTGGGTTATTGGAT+TGG | 0.512161 | 4.4:+15280658 | MS.gene09018:CDS |
| CTCAACACCGACGGATTCAC+CGG | 0.514030 | 4.4:-15280521 | None:intergenic |
| GACGGATTCACCGGCACGAT+CGG | 0.516430 | 4.4:-15280512 | None:intergenic |
| GATCGTGCCGGTGAATCCGT+CGG | 0.526152 | 4.4:+15280514 | MS.gene09018:CDS |
| TGGCGAAGCAAAGCAAAGAA+TGG | 0.536106 | 4.4:-15280735 | None:intergenic |
| ATAGATTATGATTTCTTACC+GGG | 0.542281 | 4.4:-15280770 | None:intergenic |
| GTCGGTGTTGAGACGTTCGT+CGG | 0.552778 | 4.4:+15280532 | MS.gene09018:CDS |
| CGGCACGATCGGAGATCGAT+CGG | 0.564859 | 4.4:-15280501 | None:intergenic |
| CGATCGATCTCCGATCGTGC+CGG | 0.583698 | 4.4:+15280502 | MS.gene09018:CDS |
| TGGGTTTATGTTGATGCCGT+GGG | 0.591957 | 4.4:+15280646 | MS.gene09018:CDS |
| GATCGATCGGAATAAGATAA+CGG | 0.597785 | 4.4:-15280488 | None:intergenic |
| TTGGGTTTATGTTGATGCCG+TGG | 0.602091 | 4.4:+15280645 | MS.gene09018:CDS |
| GTTTGTTCTCGGGATTTCGG+TGG | 0.621102 | 4.4:+15280589 | MS.gene09018:CDS |
| GCAGGTCAGATAACTCCGGC+GGG | 0.623444 | 4.4:+15280557 | MS.gene09018:CDS |
| GACGAACGTCTCAACACCGA+CGG | 0.630029 | 4.4:-15280530 | None:intergenic |
| GGTGTTGAGACGTTCGTCGG+CGG | 0.630502 | 4.4:+15280535 | MS.gene09018:CDS |
| TCGCCACGTAAAGATTCTCC+CGG | 0.632756 | 4.4:+15280752 | MS.gene09018:CDS |
| TCGGTGGCTTTGATGCTGTG+TGG | 0.645493 | 4.4:+15280605 | MS.gene09018:CDS |
| GGCAGGTCAGATAACTCCGG+CGG | 0.647696 | 4.4:+15280556 | MS.gene09018:CDS |
| GGCGGCAGGTCAGATAACTC+CGG | 0.648034 | 4.4:+15280553 | MS.gene09018:CDS |
| TCGGAATAAGATAACGGCGT+CGG | 0.648078 | 4.4:-15280482 | None:intergenic |
| ACAAACGACGCAAATCCCGC+CGG | 0.670719 | 4.4:-15280572 | None:intergenic |
| ATAACCAATCCAATAACCCA+CGG | 0.672738 | 4.4:-15280662 | None:intergenic |
| TTACCGGGAGAATCTTTACG+TGG | 0.685734 | 4.4:-15280755 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GTTTCTTTTCTTTCTGTTTT+GGG | + | chr4.4:15280707-15280726 | MS.gene09018:CDS | 25.0% |
| !! | TGTTTCTTTTCTTTCTGTTT+TGG | + | chr4.4:15280706-15280725 | MS.gene09018:CDS | 25.0% |
| ATAACCAATCCAATAACCCA+CGG | - | chr4.4:15280665-15280684 | None:intergenic | 35.0% | |
| GATCGATCGGAATAAGATAA+CGG | - | chr4.4:15280491-15280510 | None:intergenic | 35.0% | |
| ! | GTTATGGTGTTTTATGTTGC+TGG | + | chr4.4:15280680-15280699 | MS.gene09018:CDS | 35.0% |
| ! | GATCGGTTACTTTCGTGATT+GGG | + | chr4.4:15280627-15280646 | MS.gene09018:CDS | 40.0% |
| !! | GTGGGTTATTGGATTGGTTA+TGG | + | chr4.4:15280664-15280683 | MS.gene09018:CDS | 40.0% |
| GATTTGCGTCGTTTGTTCTC+GGG | + | chr4.4:15280579-15280598 | MS.gene09018:CDS | 45.0% | |
| GGATTTGCGTCGTTTGTTCT+CGG | + | chr4.4:15280578-15280597 | MS.gene09018:CDS | 45.0% | |
| GTCGTTTGTTCTCGGGATTT+CGG | + | chr4.4:15280586-15280605 | MS.gene09018:CDS | 45.0% | |
| TGGCGAAGCAAAGCAAAGAA+TGG | - | chr4.4:15280738-15280757 | None:intergenic | 45.0% | |
| TGGGTTTATGTTGATGCCGT+GGG | + | chr4.4:15280646-15280665 | MS.gene09018:CDS | 45.0% | |
| TTACCGGGAGAATCTTTACG+TGG | - | chr4.4:15280758-15280777 | None:intergenic | 45.0% | |
| TTGGGTTTATGTTGATGCCG+TGG | + | chr4.4:15280645-15280664 | MS.gene09018:CDS | 45.0% | |
| ! | ATGTTGATGCCGTGGGTTAT+TGG | + | chr4.4:15280653-15280672 | MS.gene09018:CDS | 45.0% |
| ! | GGATCGGTTACTTTCGTGAT+TGG | + | chr4.4:15280626-15280645 | MS.gene09018:CDS | 45.0% |
| ! | TCGGAATAAGATAACGGCGT+CGG | - | chr4.4:15280485-15280504 | None:intergenic | 45.0% |
| GTTTGTTCTCGGGATTTCGG+TGG | + | chr4.4:15280589-15280608 | MS.gene09018:CDS | 50.0% | |
| TCGCCACGTAAAGATTCTCC+CGG | + | chr4.4:15280752-15280771 | MS.gene09018:CDS | 50.0% | |
| ! | GATGCCGTGGGTTATTGGAT+TGG | + | chr4.4:15280658-15280677 | MS.gene09018:CDS | 50.0% |
| ! | GGCTTTGATGCTGTGTGGAT+CGG | + | chr4.4:15280610-15280629 | MS.gene09018:CDS | 50.0% |
| ACAAACGACGCAAATCCCGC+CGG | - | chr4.4:15280575-15280594 | None:intergenic | 55.0% | |
| GACGAACGTCTCAACACCGA+CGG | - | chr4.4:15280533-15280552 | None:intergenic | 55.0% | |
| ! | CTCAACACCGACGGATTCAC+CGG | - | chr4.4:15280524-15280543 | None:intergenic | 55.0% |
| !! | GTCGGTGTTGAGACGTTCGT+CGG | + | chr4.4:15280532-15280551 | MS.gene09018:CDS | 55.0% |
| !! | TCGGTGGCTTTGATGCTGTG+TGG | + | chr4.4:15280605-15280624 | MS.gene09018:CDS | 55.0% |
| CGATCGATCTCCGATCGTGC+CGG | + | chr4.4:15280502-15280521 | MS.gene09018:CDS | 60.0% | |
| CGGCACGATCGGAGATCGAT+CGG | - | chr4.4:15280504-15280523 | None:intergenic | 60.0% | |
| GCAGGTCAGATAACTCCGGC+GGG | + | chr4.4:15280557-15280576 | MS.gene09018:CDS | 60.0% | |
| GGCAGGTCAGATAACTCCGG+CGG | + | chr4.4:15280556-15280575 | MS.gene09018:CDS | 60.0% | |
| GGCGGCAGGTCAGATAACTC+CGG | + | chr4.4:15280553-15280572 | MS.gene09018:CDS | 60.0% | |
| ! | GACGGATTCACCGGCACGAT+CGG | - | chr4.4:15280515-15280534 | None:intergenic | 60.0% |
| ! | GATCGTGCCGGTGAATCCGT+CGG | + | chr4.4:15280514-15280533 | MS.gene09018:CDS | 60.0% |
| ! | GGTGTTGAGACGTTCGTCGG+CGG | + | chr4.4:15280535-15280554 | MS.gene09018:CDS | 60.0% |
| TTGAGACGTTCGTCGGCGGC+AGG | + | chr4.4:15280539-15280558 | MS.gene09018:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 15280392 | 15280787 | 15280392 | ID=MS.gene09018 |
| chr4.4 | mRNA | 15280392 | 15280787 | 15280392 | ID=MS.gene09018.t1;Parent=MS.gene09018 |
| chr4.4 | exon | 15280392 | 15280787 | 15280392 | ID=MS.gene09018.t1.exon1;Parent=MS.gene09018.t1 |
| chr4.4 | CDS | 15280392 | 15280787 | 15280392 | ID=cds.MS.gene09018.t1;Parent=MS.gene09018.t1 |
| Gene Sequence |
| Protein sequence |
