Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09143.t1 | XP_003606312.1 | 100 | 88 | 0 | 0 | 1 | 88 | 1 | 88 | 2.50E-44 | 187.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09143.t1 | O23249 | 94.3 | 88 | 4 | 1 | 1 | 88 | 1 | 87 | 2.4e-43 | 175.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09143.t1 | G7JM76 | 100.0 | 88 | 0 | 0 | 1 | 88 | 1 | 88 | 1.8e-44 | 187.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene09143 | MS.gene29062 | PPI |
| MS.gene09143 | MS.gene94095 | PPI |
| MS.gene09143 | MS.gene86296 | PPI |
| MS.gene09143 | MS.gene015893 | PPI |
| MS.gene09143 | MS.gene57583 | PPI |
| MS.gene73923 | MS.gene09143 | PPI |
| MS.gene09143 | MS.gene031852 | PPI |
| MS.gene09143 | MS.gene036003 | PPI |
| MS.gene09143 | MS.gene54070 | PPI |
| MS.gene09143 | MS.gene021573 | PPI |
| MS.gene09143 | MS.gene053749 | PPI |
| MS.gene09143 | MS.gene30776 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09143.t1 | MTR_4g057040 | 100.000 | 88 | 0 | 0 | 1 | 88 | 1 | 88 | 1.71e-62 | 184 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09143.t1 | AT2G27970 | 90.000 | 80 | 8 | 0 | 1 | 80 | 1 | 80 | 1.45e-51 | 156 |
| MS.gene09143.t1 | AT2G27960 | 97.183 | 71 | 2 | 0 | 1 | 71 | 1 | 71 | 5.79e-49 | 150 |
Find 26 sgRNAs with CRISPR-Local
Find 104 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCAGGACAACCAGGCCCAGC+AGG | 0.260365 | 4.1:-41217928 | MS.gene09143:CDS |
| CCAGCATGCCCTGCTGGGCC+TGG | 0.333404 | 4.1:+41217919 | None:intergenic |
| AGCCACACATTATGCTCTTC+AGG | 0.335634 | 4.1:-41217974 | MS.gene09143:CDS |
| TTCAGAGAGAAGTCGATTCT+TGG | 0.337969 | 4.1:+41220076 | None:intergenic |
| ATTTGACCAGCATGCCCTGC+TGG | 0.343644 | 4.1:+41217913 | None:intergenic |
| GAGTGCAGCAGAGCCGTGGC+TGG | 0.380036 | 4.1:-41218022 | MS.gene09143:CDS |
| CAGAATGAATGGCGCGCAAT+TGG | 0.392088 | 4.1:-41218044 | MS.gene09143:CDS |
| GGAAGCAACTTCGCAACCTC+TGG | 0.415067 | 4.1:+41220098 | None:intergenic |
| GTTTAATCTTACAGAATGAA+TGG | 0.420697 | 4.1:-41218055 | MS.gene09143:intron |
| TTTGACCAGCATGCCCTGCT+GGG | 0.452510 | 4.1:+41217914 | None:intergenic |
| TCAGAGAGAAGTCGATTCTT+GGG | 0.456322 | 4.1:+41220077 | None:intergenic |
| CGAAATACTTCTCAGAGTAC+TGG | 0.464971 | 4.1:+41220271 | None:intergenic |
| CCAGGCCCAGCAGGGCATGC+TGG | 0.487057 | 4.1:-41217919 | MS.gene09143:CDS |
| TGCTGCTGCTGATAATTCAA+TGG | 0.507626 | 4.1:+41217948 | None:intergenic |
| CAGGACAACCAGGCCCAGCA+GGG | 0.518346 | 4.1:-41217927 | MS.gene09143:CDS |
| AGTGCAGCAGAGCCGTGGCT+GGG | 0.520271 | 4.1:-41218021 | MS.gene09143:CDS |
| TCGATGACACTTATGAGTAC+AGG | 0.536322 | 4.1:-41220251 | MS.gene09143:intron |
| AAGAGCATAATGTGTGGCTC+AGG | 0.536360 | 4.1:+41217978 | None:intergenic |
| GCATGTTGTTCTTCCTCCAG+AGG | 0.545874 | 4.1:-41220114 | MS.gene09143:CDS |
| AGCAACTTCGCAACCTCTGG+AGG | 0.549766 | 4.1:+41220101 | None:intergenic |
| ATTGAATTATCAGCAGCAGC+AGG | 0.557262 | 4.1:-41217946 | MS.gene09143:CDS |
| ATTGGAGTGCAGCAGAGCCG+TGG | 0.573620 | 4.1:-41218026 | MS.gene09143:CDS |
| TCAGCAGCAGCAGGACAACC+AGG | 0.580092 | 4.1:-41217937 | MS.gene09143:CDS |
| TTGCATAATGAACCCAGCCA+CGG | 0.629727 | 4.1:+41218009 | None:intergenic |
| CACACATTATGCTCTTCAGG+AGG | 0.695794 | 4.1:-41217971 | MS.gene09143:CDS |
| CTCCTGAAGAGCATAATGTG+TGG | 0.729925 | 4.1:+41217972 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATTTTTTTTATTTTTTTTT+TGG | - | chr4.1:41218150-41218169 | MS.gene09143:intron | 0.0% |
| !!! | ATTATTTTTGAAAATAAGTA+TGG | - | chr4.1:41219987-41220006 | MS.gene09143:intron | 10.0% |
| !! | AAGTTATCAATTAAAAGTTT+AGG | + | chr4.1:41219042-41219061 | None:intergenic | 15.0% |
| !! | AGTTATCAATTAAAAGTTAA+AGG | + | chr4.1:41219013-41219032 | None:intergenic | 15.0% |
| !! | AGTTATCAATTAAAAGTTTA+AGG | + | chr4.1:41219041-41219060 | None:intergenic | 15.0% |
| !! | AGTTATCAATTAAAAGTTTA+GGG | + | chr4.1:41219041-41219060 | None:intergenic | 15.0% |
| !! | ATTAAATACACAATGAATTT+AGG | + | chr4.1:41219775-41219794 | None:intergenic | 15.0% |
| !!! | ATTTGTAAAATTTAAGTTCA+AGG | + | chr4.1:41219833-41219852 | None:intergenic | 15.0% |
| !! | AGTTATCAGTTAAAAGTTTA+AGG | + | chr4.1:41218983-41219002 | None:intergenic | 20.0% |
| !! | AGTTGTCAATTAAAAGTTTA+AGG | + | chr4.1:41219368-41219387 | None:intergenic | 20.0% |
| !! | ATACAAATTAGGAATTACTT+TGG | + | chr4.1:41219647-41219666 | None:intergenic | 20.0% |
| !!! | AATACTGTATGATTTAAGTT+AGG | + | chr4.1:41219105-41219124 | None:intergenic | 20.0% |
| !!! | GTAATTCCTAATTTGTATTT+TGG | - | chr4.1:41219649-41219668 | MS.gene09143:intron | 20.0% |
| !!! | TAATTTTCTTGTTATTGTTG+TGG | - | chr4.1:41218709-41218728 | MS.gene09143:intron | 20.0% |
| !!! | TATACTGTATGATTTAAGTT+TGG | + | chr4.1:41218956-41218975 | None:intergenic | 20.0% |
| !!! | TTTTTTTGTTGCTGTTTTAT+GGG | - | chr4.1:41218555-41218574 | MS.gene09143:intron | 20.0% |
| !!! | TTTTTTTTGTTGCTGTTTTA+TGG | - | chr4.1:41218554-41218573 | MS.gene09143:intron | 20.0% |
| ! | AAAACCAAATTAAATCACTG+TGG | + | chr4.1:41219491-41219510 | None:intergenic | 25.0% |
| ! | AAACTTCAAATCACTGTATT+TGG | + | chr4.1:41219565-41219584 | None:intergenic | 25.0% |
| ! | AAAGTGAATTCAGTGAATTA+GGG | + | chr4.1:41218919-41218938 | None:intergenic | 25.0% |
| ! | AACATGCCAAAATACAAATT+AGG | + | chr4.1:41219658-41219677 | None:intergenic | 25.0% |
| ! | AATCACTGTATTTGGATATT+AGG | + | chr4.1:41219557-41219576 | None:intergenic | 25.0% |
| ! | AGTTCATTACACAGTAAATT+TGG | + | chr4.1:41218504-41218523 | None:intergenic | 25.0% |
| ! | GTATTCAGTTATCAGTTAAA+AGG | + | chr4.1:41219129-41219148 | None:intergenic | 25.0% |
| ! | GTTCATTACACAGTAAATTT+GGG | + | chr4.1:41218503-41218522 | None:intergenic | 25.0% |
| ! | GTTTAATCTTACAGAATGAA+TGG | - | chr4.1:41220138-41220157 | MS.gene09143:intron | 25.0% |
| ! | TCAAAATAGATAATACCCTA+AGG | + | chr4.1:41218689-41218708 | None:intergenic | 25.0% |
| ! | TGAAGACTTATATTTGAATC+TGG | - | chr4.1:41218420-41218439 | MS.gene09143:intron | 25.0% |
| ! | TTGAGACGTATATTTGAATT+TGG | - | chr4.1:41218809-41218828 | MS.gene09143:intron | 25.0% |
| !! | TGGGTTTAAAAATCATTTTG+AGG | + | chr4.1:41218484-41218503 | None:intergenic | 25.0% |
| !!! | AATGTTAATTTGTGCATGTT+AGG | - | chr4.1:41218370-41218389 | MS.gene09143:intron | 25.0% |
| !!! | ACAGTGATTTAATTTGGTTT+TGG | - | chr4.1:41219490-41219509 | MS.gene09143:intron | 25.0% |
| !!! | ATGATTTAGGGTTTTGTTAT+GGG | - | chr4.1:41220055-41220074 | MS.gene09143:intron | 25.0% |
| !!! | ATGTTAATTTGTGCATGTTA+GGG | - | chr4.1:41218371-41218390 | MS.gene09143:intron | 25.0% |
| !!! | TATGATTTAGGGTTTTGTTA+TGG | - | chr4.1:41220054-41220073 | MS.gene09143:intron | 25.0% |
| !!! | TATGTTTTAGCGTTTAGATT+AGG | - | chr4.1:41219513-41219532 | MS.gene09143:intron | 25.0% |
| !!! | TGTTTCGTTTTTGTTTTTTC+AGG | - | chr4.1:41218057-41218076 | MS.gene09143:intron | 25.0% |
| AAGCTGAAGCTATATGATTT+AGG | - | chr4.1:41220042-41220061 | MS.gene09143:intron | 30.0% | |
| AATATTACCTTGTGCATGAT+TGG | - | chr4.1:41219447-41219466 | MS.gene09143:intron | 30.0% | |
| AGCTGAAGCTATATGATTTA+GGG | - | chr4.1:41220043-41220062 | MS.gene09143:intron | 30.0% | |
| GAAAGTGAATTCAGTGAATT+AGG | + | chr4.1:41218920-41218939 | None:intergenic | 30.0% | |
| GGCATAAAAATCATGTTTAG+AGG | + | chr4.1:41219754-41219773 | None:intergenic | 30.0% | |
| GTATTATCACTGTTGAAAGA+GGG | - | chr4.1:41218775-41218794 | MS.gene09143:intron | 30.0% | |
| TCGGTTTAATATGGAGATAA+TGG | + | chr4.1:41219870-41219889 | None:intergenic | 30.0% | |
| ! | AAAAGTTTAAGGTATGCACA+GGG | + | chr4.1:41219407-41219426 | None:intergenic | 30.0% |
| ! | AAACTACAAATCCAAGCTTT+TGG | + | chr4.1:41218887-41218906 | None:intergenic | 30.0% |
| ! | GTTACTCTGATGATGAAATT+GGG | - | chr4.1:41218271-41218290 | MS.gene09143:intron | 30.0% |
| ! | TAAAAGTTTAAGGTATGCAC+AGG | + | chr4.1:41219408-41219427 | None:intergenic | 30.0% |
| ! | TGTTACTCTGATGATGAAAT+TGG | - | chr4.1:41218270-41218289 | MS.gene09143:intron | 30.0% |
| ! | TTACTCTGATGATGAAATTG+GGG | - | chr4.1:41218272-41218291 | MS.gene09143:intron | 30.0% |
| !! | TAGCATTCTCACTTTTGAAA+GGG | - | chr4.1:41219620-41219639 | MS.gene09143:intron | 30.0% |
| !! | TTAGCATTCTCACTTTTGAA+AGG | - | chr4.1:41219619-41219638 | MS.gene09143:intron | 30.0% |
| AACACACTCAATCAAGGAAA+TGG | - | chr4.1:41219889-41219908 | MS.gene09143:intron | 35.0% | |
| AATATTACCCTGTGCATGAT+TGG | - | chr4.1:41219062-41219081 | MS.gene09143:intron | 35.0% | |
| GAGACCACAGTGATTTAATT+TGG | - | chr4.1:41219484-41219503 | MS.gene09143:intron | 35.0% | |
| GGTATTATCACTGTTGAAAG+AGG | - | chr4.1:41218774-41218793 | MS.gene09143:intron | 35.0% | |
| GTTGAGTCTTCTGATGAAAA+TGG | - | chr4.1:41218610-41218629 | MS.gene09143:intron | 35.0% | |
| TAATCCTGGTTAAGTCCTTA+GGG | - | chr4.1:41218671-41218690 | MS.gene09143:intron | 35.0% | |
| TGAGTGTGTTCGGTTTAATA+TGG | + | chr4.1:41219879-41219898 | None:intergenic | 35.0% | |
| TTAATCCTGGTTAAGTCCTT+AGG | - | chr4.1:41218670-41218689 | MS.gene09143:intron | 35.0% | |
| TTGGCATGTTTGAGAATTCT+AGG | - | chr4.1:41219668-41219687 | MS.gene09143:intron | 35.0% | |
| TTTCCTTGATTGAGTGTGTT+CGG | + | chr4.1:41219889-41219908 | None:intergenic | 35.0% | |
| ! | AAACAACAACTCTTGGCTTT+AGG | + | chr4.1:41219728-41219747 | None:intergenic | 35.0% |
| ! | ATCGATTCTAACCAAAAGCT+TGG | - | chr4.1:41218873-41218892 | MS.gene09143:intron | 35.0% |
| ! | ATGTTAGGGTGTACTTTTGA+AGG | - | chr4.1:41218385-41218404 | MS.gene09143:intron | 35.0% |
| ! | TCATGATGATGACTTAATCC+TGG | - | chr4.1:41218657-41218676 | MS.gene09143:intron | 35.0% |
| ! | TGTTTTATGGGTATGTGTGA+TGG | - | chr4.1:41218567-41218586 | MS.gene09143:intron | 35.0% |
| ! | TTGTTGTGGTATTTGATAGC+TGG | - | chr4.1:41218723-41218742 | MS.gene09143:intron | 35.0% |
| !! | AGCATTCTCACTTTTGAAAG+GGG | - | chr4.1:41219621-41219640 | MS.gene09143:intron | 35.0% |
| AAACCGAACACACTCAATCA+AGG | - | chr4.1:41219883-41219902 | MS.gene09143:intron | 40.0% | |
| AAGTGAATCCAATCATGCAC+AGG | + | chr4.1:41219073-41219092 | None:intergenic | 40.0% | |
| AATACCCTAAGGACTTAACC+AGG | + | chr4.1:41218678-41218697 | None:intergenic | 40.0% | |
| AGCTTCAGCTTTGCATTGAA+TGG | + | chr4.1:41220033-41220052 | None:intergenic | 40.0% | |
| AGTGAATCCAATCATGCACA+AGG | + | chr4.1:41219072-41219091 | None:intergenic | 40.0% | |
| AGTGAATCCAATCATGCACA+GGG | + | chr4.1:41219072-41219091 | None:intergenic | 40.0% | |
| ATTGAATTATCAGCAGCAGC+AGG | - | chr4.1:41220247-41220266 | MS.gene09143:intron | 40.0% | |
| CGAAATACTTCTCAGAGTAC+TGG | + | chr4.1:41217925-41217944 | None:intergenic | 40.0% | |
| GAGGCAAAAACAACAACTCT+TGG | + | chr4.1:41219735-41219754 | None:intergenic | 40.0% | |
| GATAGCTGGATATTACTGTG+TGG | - | chr4.1:41218737-41218756 | MS.gene09143:intron | 40.0% | |
| TCGATGACACTTATGAGTAC+AGG | - | chr4.1:41217942-41217961 | MS.gene09143:CDS | 40.0% | |
| TGCTGCTGCTGATAATTCAA+TGG | + | chr4.1:41220248-41220267 | None:intergenic | 40.0% | |
| TGTGTGGCATGTTATTGTTG+TGG | - | chr4.1:41218753-41218772 | MS.gene09143:intron | 40.0% | |
| ! | TCAGAGAGAAGTCGATTCTT+GGG | + | chr4.1:41218119-41218138 | None:intergenic | 40.0% |
| ! | TTCAGAGAGAAGTCGATTCT+TGG | + | chr4.1:41218120-41218139 | None:intergenic | 40.0% |
| AGCCACACATTATGCTCTTC+AGG | - | chr4.1:41220219-41220238 | MS.gene09143:intron | 45.0% | |
| CACACATTATGCTCTTCAGG+AGG | - | chr4.1:41220222-41220241 | MS.gene09143:intron | 45.0% | |
| TTGCATAATGAACCCAGCCA+CGG | + | chr4.1:41220187-41220206 | None:intergenic | 45.0% | |
| ! | AAGAGCATAATGTGTGGCTC+AGG | + | chr4.1:41220218-41220237 | None:intergenic | 45.0% |
| ! | CTCCTGAAGAGCATAATGTG+TGG | + | chr4.1:41220224-41220243 | None:intergenic | 45.0% |
| !!! | TTTTTATTATTATTTTTTTG+TGG | - | chr4.1:41218334-41218353 | MS.gene09143:intron | 5.0% |
| CAGAATGAATGGCGCGCAAT+TGG | - | chr4.1:41220149-41220168 | MS.gene09143:intron | 50.0% | |
| GCATGTTGTTCTTCCTCCAG+AGG | - | chr4.1:41218079-41218098 | MS.gene09143:intron | 50.0% | |
| AGCAACTTCGCAACCTCTGG+AGG | + | chr4.1:41218095-41218114 | None:intergenic | 55.0% | |
| ATTTGACCAGCATGCCCTGC+TGG | + | chr4.1:41220283-41220302 | None:intergenic | 55.0% | |
| GGAAGCAACTTCGCAACCTC+TGG | + | chr4.1:41218098-41218117 | None:intergenic | 55.0% | |
| TTTGACCAGCATGCCCTGCT+GGG | + | chr4.1:41220282-41220301 | None:intergenic | 55.0% | |
| ATTGGAGTGCAGCAGAGCCG+TGG | - | chr4.1:41220167-41220186 | MS.gene09143:intron | 60.0% | |
| TCAGCAGCAGCAGGACAACC+AGG | - | chr4.1:41220256-41220275 | MS.gene09143:CDS | 60.0% | |
| AGTGCAGCAGAGCCGTGGCT+GGG | - | chr4.1:41220172-41220191 | MS.gene09143:intron | 65.0% | |
| CAGGACAACCAGGCCCAGCA+GGG | - | chr4.1:41220266-41220285 | MS.gene09143:CDS | 65.0% | |
| GAGTGCAGCAGAGCCGTGGC+TGG | - | chr4.1:41220171-41220190 | MS.gene09143:intron | 70.0% | |
| GCAGGACAACCAGGCCCAGC+AGG | - | chr4.1:41220265-41220284 | MS.gene09143:CDS | 70.0% | |
| CCAGCATGCCCTGCTGGGCC+TGG | + | chr4.1:41220277-41220296 | None:intergenic | 75.0% | |
| CCAGGCCCAGCAGGGCATGC+TGG | - | chr4.1:41220274-41220293 | MS.gene09143:CDS | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 41217911 | 41220304 | 41217911 | ID=MS.gene09143 |
| chr4.1 | mRNA | 41217911 | 41220304 | 41217911 | ID=MS.gene09143.t1;Parent=MS.gene09143 |
| chr4.1 | exon | 41220252 | 41220304 | 41220252 | ID=MS.gene09143.t1.exon1;Parent=MS.gene09143.t1 |
| chr4.1 | CDS | 41220252 | 41220304 | 41220252 | ID=cds.MS.gene09143.t1;Parent=MS.gene09143.t1 |
| chr4.1 | exon | 41220076 | 41220136 | 41220076 | ID=MS.gene09143.t1.exon2;Parent=MS.gene09143.t1 |
| chr4.1 | CDS | 41220076 | 41220136 | 41220076 | ID=cds.MS.gene09143.t1;Parent=MS.gene09143.t1 |
| chr4.1 | exon | 41217911 | 41218063 | 41217911 | ID=MS.gene09143.t1.exon3;Parent=MS.gene09143.t1 |
| chr4.1 | CDS | 41217911 | 41218063 | 41217911 | ID=cds.MS.gene09143.t1;Parent=MS.gene09143.t1 |
| Gene Sequence |
| Protein sequence |
