AlfalfaGEDB | MS.gene09227

M. sativa cultivar XinJiangDaYe / MS.gene09227

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene09227.t1	RHN42498.1	95.3	127	6	0	1	127	82	208	7.30E-48	199.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene09227.t1	G7LH97	96.0	126	5	0	2	127	1	126	5.3e-48	199.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050463	MS.gene09227	0.862809	3.90E-64	-1.69E-46
MS.gene050528	MS.gene09227	0.860466	2.03E-63	-1.69E-46
MS.gene051946	MS.gene09227	0.815838	7.53E-52	-1.69E-46
MS.gene053175	MS.gene09227	0.801895	7.26E-49	-1.69E-46
MS.gene057014	MS.gene09227	0.802695	4.97E-49	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene09227.t1	MTR_8g097040	94.574	129	7	0	2	130	1	129	1.02e-84	243
MS.gene09227.t1	MTR_3g084990	55.147	136	47	4	1	130	1	128	2.77e-42	136
MS.gene09227.t1	MTR_5g078400	54.054	74	27	2	17	90	5	71	1.23e-16	69.7
MS.gene09227.t1	MTR_7g028805	42.308	130	60	3	1	117	1	128	1.30e-16	71.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene09227.t1	AT1G27290	51.825	137	57	4	1	130	1	135	8.83e-38	125
MS.gene09227.t1	AT1G27290	51.825	137	57	4	1	130	1	135	8.83e-38	125

Find 34 sgRNAs with CRISPR-Local

Find 35 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GGATCGGTTACTTTCGTGAT+TGG	0.305883	4.1:+14964228	MS.gene09227:CDS
ATGTTGATGCCGTGGGTTAT+TGG	0.333475	4.1:+14964255	MS.gene09227:CDS
CATAGATTATGATTTCTTAC+CGG	0.352525	4.1:-14964373	None:intergenic
GTGGGTTATTGGATTGGTTA+TGG	0.372418	4.1:+14964266	MS.gene09227:CDS
GGATTTGCGTCGTTTGTTCT+CGG	0.413462	4.1:+14964180	MS.gene09227:CDS
GATTTGCGTCGTTTGTTCTC+GGG	0.415303	4.1:+14964181	MS.gene09227:CDS
GTCGTTTGTTCTCGGGATTT+CGG	0.429794	4.1:+14964188	MS.gene09227:CDS
GATCGGTTACTTTCGTGATT+GGG	0.431361	4.1:+14964229	MS.gene09227:CDS
TTGAGACGTTCGTCGGCGGC+AGG	0.478548	4.1:+14964141	MS.gene09227:CDS
GGCTTTGATGCTGTGTGGAT+CGG	0.501281	4.1:+14964212	MS.gene09227:CDS
GATGCCGTGGGTTATTGGAT+TGG	0.512161	4.1:+14964260	MS.gene09227:CDS
CTCAACACCGACGGATTCAC+CGG	0.514030	4.1:-14964123	None:intergenic
GACGGATTCACCGGCACGAT+CGG	0.516430	4.1:-14964114	None:intergenic
GATCGTGCCGGTGAATCCGT+CGG	0.526152	4.1:+14964116	MS.gene09227:CDS
TGGCGAAGCAAAGCAAAGAA+TGG	0.536106	4.1:-14964337	None:intergenic
ATAGATTATGATTTCTTACC+GGG	0.542281	4.1:-14964372	None:intergenic
GTCGGTGTTGAGACGTTCGT+CGG	0.552778	4.1:+14964134	MS.gene09227:CDS
CGGCACGATCGGAGATCGAT+CGG	0.564859	4.1:-14964103	None:intergenic
CGATCGATCTCCGATCGTGC+CGG	0.583698	4.1:+14964104	MS.gene09227:CDS
TGGGTTTATGTTGATGCCGT+GGG	0.591957	4.1:+14964248	MS.gene09227:CDS
GATCGATCGGAATAAGATAA+CGG	0.597785	4.1:-14964090	None:intergenic
TTGGGTTTATGTTGATGCCG+TGG	0.602091	4.1:+14964247	MS.gene09227:CDS
GTTTGTTCTCGGGATTTCGG+TGG	0.621102	4.1:+14964191	MS.gene09227:CDS
GCAGGTCAGATAACTCCGGC+GGG	0.623444	4.1:+14964159	MS.gene09227:CDS
GACGAACGTCTCAACACCGA+CGG	0.630029	4.1:-14964132	None:intergenic
GGTGTTGAGACGTTCGTCGG+CGG	0.630502	4.1:+14964137	MS.gene09227:CDS
TCGCCACGTAAAGATTCTCC+CGG	0.632756	4.1:+14964354	MS.gene09227:CDS
TCGGTGGCTTTGATGCTGTG+TGG	0.645493	4.1:+14964207	MS.gene09227:CDS
GGCAGGTCAGATAACTCCGG+CGG	0.647696	4.1:+14964158	MS.gene09227:CDS
GGCGGCAGGTCAGATAACTC+CGG	0.648034	4.1:+14964155	MS.gene09227:CDS
TCGGAATAAGATAACGGCGT+CGG	0.648078	4.1:-14964084	None:intergenic
ACAAACGACGCAAATCCCGC+CGG	0.670719	4.1:-14964174	None:intergenic
ATAACCAATCCAATAACCCA+CGG	0.672738	4.1:-14964264	None:intergenic
TTACCGGGAGAATCTTTACG+TGG	0.685734	4.1:-14964357	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	GTTTCTTTTCTTTCTGTTTT+GGG	+	chr4.1:14964309-14964328	MS.gene09227:CDS	25.0%
!!	TGTTTCTTTTCTTTCTGTTT+TGG	+	chr4.1:14964308-14964327	MS.gene09227:CDS	25.0%
	ATAACCAATCCAATAACCCA+CGG	-	chr4.1:14964267-14964286	None:intergenic	35.0%
	GATCGATCGGAATAAGATAA+CGG	-	chr4.1:14964093-14964112	None:intergenic	35.0%
!	GTTATGGTGTTTTATGTTGC+TGG	+	chr4.1:14964282-14964301	MS.gene09227:CDS	35.0%
!	GATCGGTTACTTTCGTGATT+GGG	+	chr4.1:14964229-14964248	MS.gene09227:CDS	40.0%
!!	GTGGGTTATTGGATTGGTTA+TGG	+	chr4.1:14964266-14964285	MS.gene09227:CDS	40.0%
	GATTTGCGTCGTTTGTTCTC+GGG	+	chr4.1:14964181-14964200	MS.gene09227:CDS	45.0%
	GGATTTGCGTCGTTTGTTCT+CGG	+	chr4.1:14964180-14964199	MS.gene09227:CDS	45.0%
	GTCGTTTGTTCTCGGGATTT+CGG	+	chr4.1:14964188-14964207	MS.gene09227:CDS	45.0%
	TGGCGAAGCAAAGCAAAGAA+TGG	-	chr4.1:14964340-14964359	None:intergenic	45.0%
	TGGGTTTATGTTGATGCCGT+GGG	+	chr4.1:14964248-14964267	MS.gene09227:CDS	45.0%
	TTACCGGGAGAATCTTTACG+TGG	-	chr4.1:14964360-14964379	None:intergenic	45.0%
	TTGGGTTTATGTTGATGCCG+TGG	+	chr4.1:14964247-14964266	MS.gene09227:CDS	45.0%
!	ATGTTGATGCCGTGGGTTAT+TGG	+	chr4.1:14964255-14964274	MS.gene09227:CDS	45.0%
!	GGATCGGTTACTTTCGTGAT+TGG	+	chr4.1:14964228-14964247	MS.gene09227:CDS	45.0%
!	TCGGAATAAGATAACGGCGT+CGG	-	chr4.1:14964087-14964106	None:intergenic	45.0%
	GTTTGTTCTCGGGATTTCGG+TGG	+	chr4.1:14964191-14964210	MS.gene09227:CDS	50.0%
	TCGCCACGTAAAGATTCTCC+CGG	+	chr4.1:14964354-14964373	MS.gene09227:CDS	50.0%
!	GATGCCGTGGGTTATTGGAT+TGG	+	chr4.1:14964260-14964279	MS.gene09227:CDS	50.0%
!	GGCTTTGATGCTGTGTGGAT+CGG	+	chr4.1:14964212-14964231	MS.gene09227:CDS	50.0%
	ACAAACGACGCAAATCCCGC+CGG	-	chr4.1:14964177-14964196	None:intergenic	55.0%
	GACGAACGTCTCAACACCGA+CGG	-	chr4.1:14964135-14964154	None:intergenic	55.0%
!	CTCAACACCGACGGATTCAC+CGG	-	chr4.1:14964126-14964145	None:intergenic	55.0%
!!	GTCGGTGTTGAGACGTTCGT+CGG	+	chr4.1:14964134-14964153	MS.gene09227:CDS	55.0%
!!	TCGGTGGCTTTGATGCTGTG+TGG	+	chr4.1:14964207-14964226	MS.gene09227:CDS	55.0%
	CGATCGATCTCCGATCGTGC+CGG	+	chr4.1:14964104-14964123	MS.gene09227:CDS	60.0%
	CGGCACGATCGGAGATCGAT+CGG	-	chr4.1:14964106-14964125	None:intergenic	60.0%
	GCAGGTCAGATAACTCCGGC+GGG	+	chr4.1:14964159-14964178	MS.gene09227:CDS	60.0%
	GGCAGGTCAGATAACTCCGG+CGG	+	chr4.1:14964158-14964177	MS.gene09227:CDS	60.0%
	GGCGGCAGGTCAGATAACTC+CGG	+	chr4.1:14964155-14964174	MS.gene09227:CDS	60.0%
!	GACGGATTCACCGGCACGAT+CGG	-	chr4.1:14964117-14964136	None:intergenic	60.0%
!	GATCGTGCCGGTGAATCCGT+CGG	+	chr4.1:14964116-14964135	MS.gene09227:CDS	60.0%
!	GGTGTTGAGACGTTCGTCGG+CGG	+	chr4.1:14964137-14964156	MS.gene09227:CDS	60.0%
	TTGAGACGTTCGTCGGCGGC+AGG	+	chr4.1:14964141-14964160	MS.gene09227:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.1	gene	14963994	14964389	14963994	ID=MS.gene09227
chr4.1	mRNA	14963994	14964389	14963994	ID=MS.gene09227.t1;Parent=MS.gene09227
chr4.1	exon	14963994	14964389	14963994	ID=MS.gene09227.t1.exon1;Parent=MS.gene09227.t1
chr4.1	CDS	14963994	14964389	14963994	ID=cds.MS.gene09227.t1;Parent=MS.gene09227.t1

Gene Sequence

>MS.gene09227

ATGATGAGAAGAAATCAAGATCAAGAACAACAATCAAAGATGATTTGCGAATTATCATCTCTACTTTTCAATCTTCTTCTACAATCTTCACCGACGCCGTTATCTTATTCCGATCGATCTCCGATCGTGCCGGTGAATCCGTCGGTGTTGAGACGTTCGTCGGCGGCAGGTCAGATAACTCCGGCGGGATTTGCGTCGTTTGTTCTCGGGATTTCGGTGGCTTTGATGCTGTGTGGATCGGTTACTTTCGTGATTGGGTTTATGTTGATGCCGTGGGTTATTGGATTGGTTATGGTGTTTTATGTTGCTGGTGTTGTTTCTTTTCTTTCTGTTTTGGGTCGCTCCATTCTTTGCTTTGCTTCGCCACGTAAAGATTCTCCCGGTAAGAAATCATAA

Protein sequence

>MS.gene09227.t1

MMRRNQDQEQQSKMICELSSLLFNLLLQSSPTPLSYSDRSPIVPVNPSVLRRSSAAGQITPAGFASFVLGISVALMLCGSVTFVIGFMLMPWVIGLVMVFYVAGVVSFLSVLGRSILCFASPRKDSPGKKS