AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene09579


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene09579.t1 MTR_5g015880 95.671 231 10 0 1 231 1 231 4.55e-164 452
MS.gene09579.t1 MTR_4g095600 70.124 241 54 6 1 231 1 233 6.63e-111 318
MS.gene09579.t1 MTR_1g106420 55.921 152 62 3 1 147 1 152 7.59e-54 172
MS.gene09579.t1 MTR_7g007010 64.545 110 39 0 1 110 3 112 5.98e-52 170
MS.gene09579.t1 MTR_6g018270 61.818 110 42 0 1 110 3 112 2.00e-49 163
MS.gene09579.t1 MTR_6g453130 48.684 152 71 4 1 147 3 152 8.00e-46 152
MS.gene09579.t1 MTR_1g110840 51.128 133 61 2 1 131 3 133 7.43e-45 150
MS.gene09579.t1 MTR_6g054150 48.120 133 65 3 1 131 3 133 1.15e-40 139
MS.gene09579.t1 MTR_6g054080 47.368 133 66 3 1 131 3 133 1.41e-39 136
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene09579.t1 AT4G37540 56.048 248 84 8 1 231 1 240 9.69e-82 244
MS.gene09579.t1 AT3G49940 56.641 256 77 10 1 231 1 247 1.48e-79 239
MS.gene09579.t1 AT5G67420 52.549 255 92 6 1 231 1 250 2.72e-74 226
MS.gene09579.t1 AT5G67420 52.549 255 92 6 1 231 1 250 2.72e-74 226
MS.gene09579.t1 AT1G67100 64.545 110 39 0 1 110 3 112 5.04e-51 166
MS.gene09579.t1 AT3G02550 63.636 110 40 0 1 110 3 112 3.03e-49 162
MS.gene09579.t1 AT1G68510 60.177 113 43 1 1 111 3 115 8.51e-47 155

Find 76 sgRNAs with CRISPR-Local

Find 84 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACTCATGAACCGGACTTTC+TGG 0.160468 5.2:-7607789 None:intergenic
GAAACTCCGGTAAGGGTTTA+AGG 0.160922 5.2:-7607678 None:intergenic
ACGCGTTGACCAACGGTTTC+CGG 0.249914 5.2:+7607896 MS.gene09579:CDS
ATTAAAATTCTCAAACAAAA+AGG 0.262922 5.2:-7608044 None:intergenic
GTGGCATGACCTTGAGCTTC+AGG 0.279564 5.2:-7607303 None:intergenic
ACTCACTACAACCCTTTCTA+AGG 0.331551 5.2:-7607242 None:intergenic
GAATTCATCGACGGTGTTCC+CGG 0.351208 5.2:-7607934 None:intergenic
GGACTTTCTGGTTCGGATCT+CGG 0.355586 5.2:-7607777 None:intergenic
AGTCCGACAGCACCGTTAAC+GGG 0.370602 5.2:-7607592 None:intergenic
ACGCGTTCTGTGGCGTTAAC+CGG 0.381628 5.2:-7607879 None:intergenic
CGGAACCCTTAAACCCTTAC+CGG 0.384595 5.2:+7607672 MS.gene09579:CDS
AAAGTCCGGTTCATGAGTTC+TGG 0.390533 5.2:+7607793 MS.gene09579:CDS
TATTACGCCCTTGTATTCAA+TGG 0.394479 5.2:+7607271 MS.gene09579:CDS
AAGTCCGACAGCACCGTTAA+CGG 0.413291 5.2:-7607593 None:intergenic
AAACTCCGGTAAGGGTTTAA+GGG 0.432686 5.2:-7607677 None:intergenic
AAACCCTTACCGGAGTTTCT+CGG 0.442329 5.2:+7607682 MS.gene09579:CDS
CGGTTGCCGTGTCCTTAGAA+AGG 0.456637 5.2:+7607230 MS.gene09579:CDS
GGTGGTAAACGCAAGCGATC+AGG 0.465080 5.2:+7607814 MS.gene09579:CDS
TACCACCAGAACTCATGAAC+CGG 0.478203 5.2:-7607798 None:intergenic
ATGAACCGGACTTTCTGGTT+CGG 0.483553 5.2:-7607784 None:intergenic
AGCACCGTTAACGGGGTTCA+CGG 0.485215 5.2:-7607584 None:intergenic
TGTGACGACGGTGACGTGCT+TGG 0.492180 5.2:+7607966 MS.gene09579:CDS
TGGCATGACCTTGAGCTTCA+GGG 0.492713 5.2:-7607302 None:intergenic
GTTGAAACGGTGCTCGGTGG+CGG 0.492913 5.2:+7607652 MS.gene09579:CDS
CGGAGGGAAATTCGCCGGCC+GGG 0.499369 5.2:+7607916 MS.gene09579:CDS
AGAACCGTGAACCCCGTTAA+CGG 0.510533 5.2:+7607580 MS.gene09579:CDS
CCGGAGGGAAATTCGCCGGC+CGG 0.514516 5.2:+7607915 MS.gene09579:CDS
GTGTCAAGCGGCGGTTGAAA+CGG 0.518728 5.2:+7607639 MS.gene09579:CDS
TTACCAGGTCTCTGTGGTGA+AGG 0.520683 5.2:-7607384 None:intergenic
GGTTGCCGTGTCCTTAGAAA+GGG 0.520856 5.2:+7607231 MS.gene09579:CDS
CGTTAGAAATGAAAGACATA+AGG 0.526325 5.2:-7607359 None:intergenic
GCGTCTAAACCGAGAAACTC+CGG 0.534500 5.2:-7607691 None:intergenic
GAGATCCGAACCAGAAAGTC+CGG 0.539752 5.2:+7607779 MS.gene09579:CDS
TCATCGACGGTGTTCCCGGC+CGG 0.542141 5.2:-7607930 None:intergenic
ACGGTGACGTGCTTGGAAAG+TGG 0.543942 5.2:+7607973 MS.gene09579:CDS
GTATCGATCCATTGAATACA+AGG 0.548656 5.2:-7607279 None:intergenic
CGGTGACGTGCTTGGAAAGT+GGG 0.551329 5.2:+7607974 MS.gene09579:CDS
TACCAGGTCTCTGTGGTGAA+GGG 0.560196 5.2:-7607383 None:intergenic
TTCTGAGGAATCTGTGACGA+CGG 0.564218 5.2:+7607954 MS.gene09579:CDS
TGCTTGGAAAGTGGGATCGG+AGG 0.566383 5.2:+7607982 MS.gene09579:CDS
AAACCGAGAAACTCCGGTAA+GGG 0.570584 5.2:-7607685 None:intergenic
TAAACCGAGAAACTCCGGTA+AGG 0.571879 5.2:-7607686 None:intergenic
CCGTTGGTCAACGCGTTCTG+TGG 0.575430 5.2:-7607889 None:intergenic
GCGGCGGTTGAAACGGTGCT+CGG 0.577386 5.2:+7607646 MS.gene09579:CDS
GTTTCCGGAGGGAAATTCGC+CGG 0.577846 5.2:+7607911 MS.gene09579:CDS
AATTTCCCTCCGGAAACCGT+TGG 0.583258 5.2:-7607905 None:intergenic
ACCGTTGATGACTCATCTGA+AGG 0.585962 5.2:+7607724 MS.gene09579:CDS
ACGTGCTTGGAAAGTGGGAT+CGG 0.586938 5.2:+7607979 MS.gene09579:CDS
CCGGCCGGCGAATTTCCCTC+CGG 0.587093 5.2:-7607915 None:intergenic
AACCCCGTTAACGGTGCTGT+CGG 0.588780 5.2:+7607589 MS.gene09579:CDS
GTCCCTTCACCACAGAGACC+TGG 0.592710 5.2:+7607381 MS.gene09579:CDS
AGATTAAGATCAGTCGTCGC+CGG 0.593377 5.2:-7607856 None:intergenic
TCCTTCAGATGAGTCATCAA+CGG 0.596231 5.2:-7607725 None:intergenic
CGACGACTGATCTTAATCTC+CGG 0.598907 5.2:+7607860 MS.gene09579:CDS
AAGTCACGTGCAACGACGCG+CGG 0.599047 5.2:+7607749 MS.gene09579:CDS
CGTTGACCAACGGTTTCCGG+AGG 0.601993 5.2:+7607899 MS.gene09579:CDS
CCACAGAACGCGTTGACCAA+CGG 0.603462 5.2:+7607889 MS.gene09579:CDS
GGCATGACCTTGAGCTTCAG+GGG 0.607961 5.2:-7607301 None:intergenic
TACAACCCTTTCTAAGGACA+CGG 0.609661 5.2:-7607236 None:intergenic
ATCGATACCCCTGAAGCTCA+AGG 0.611816 5.2:+7607294 MS.gene09579:CDS
CAACTAGGTATGAGTTGCAA+CGG 0.621586 5.2:+7607210 None:intergenic
GTCCGGTTCATGAGTTCTGG+TGG 0.622596 5.2:+7607796 MS.gene09579:CDS
TGAAAGACATAAGGTCAGCA+CGG 0.634887 5.2:-7607350 None:intergenic
GTCCGACAGCACCGTTAACG+GGG 0.636214 5.2:-7607591 None:intergenic
AAACTGGCACGTGTGTCAAG+CGG 0.645348 5.2:+7607627 MS.gene09579:CDS
GTTGACCAACGGTTTCCGGA+GGG 0.646523 5.2:+7607900 MS.gene09579:CDS
CTGGCACGTGTGTCAAGCGG+CGG 0.654365 5.2:+7607630 MS.gene09579:CDS
TTCAGATGAGTCATCAACGG+TGG 0.656087 5.2:-7607722 None:intergenic
GCGGTTGAAACGGTGCTCGG+TGG 0.657002 5.2:+7607649 MS.gene09579:CDS
GATTATTTACCAGGTCTCTG+TGG 0.665083 5.2:-7607390 None:intergenic
TCTTTGTTGTTTGAAGCGTG+TGG 0.669278 5.2:+7607556 MS.gene09579:CDS
GATTCCTCAGAATTCATCGA+CGG 0.685600 5.2:-7607943 None:intergenic
TATCGATCCATTGAATACAA+GGG 0.699979 5.2:-7607278 None:intergenic
AACACCGTCGATGAATTCTG+AGG 0.704407 5.2:+7607939 MS.gene09579:CDS
AAACTTGGCTACAAAGACAG+TGG 0.714204 5.2:-7607322 None:intergenic
GGAGATAACTACGTTCACGA+CGG 0.740228 5.2:+7608003 MS.gene09579:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! GAAGATAAAGATTATTTACC+AGG - chr5.2:7607402-7607421 None:intergenic 25.0%
! TAGATGAACTAAACTAACTA+AGG + chr5.2:7607502-7607521 MS.gene09579:intron 25.0%
CGTTAGAAATGAAAGACATA+AGG - chr5.2:7607362-7607381 None:intergenic 30.0%
TATCGATCCATTGAATACAA+GGG - chr5.2:7607281-7607300 None:intergenic 30.0%
CAAAAAGGTTGAGAACTTTG+CGG - chr5.2:7608032-7608051 None:intergenic 35.0%
TATTACGCCCTTGTATTCAA+TGG + chr5.2:7607271-7607290 MS.gene09579:CDS 35.0%
! GTATCGATCCATTGAATACA+AGG - chr5.2:7607282-7607301 None:intergenic 35.0%
! GTCTTTGTAGCCAAGTTTTT+CGG + chr5.2:7607327-7607346 MS.gene09579:CDS 35.0%
! TTAGAGTGAAAGTGTAACTG+TGG - chr5.2:7607436-7607455 None:intergenic 35.0%
AAACTCCGGTAAGGGTTTAA+GGG - chr5.2:7607680-7607699 None:intergenic 40.0%
AAACTTGGCTACAAAGACAG+TGG - chr5.2:7607325-7607344 None:intergenic 40.0%
ACTCACTACAACCCTTTCTA+AGG - chr5.2:7607245-7607264 None:intergenic 40.0%
GATTATTTACCAGGTCTCTG+TGG - chr5.2:7607393-7607412 None:intergenic 40.0%
GATTCCTCAGAATTCATCGA+CGG - chr5.2:7607946-7607965 None:intergenic 40.0%
TCCTTCAGATGAGTCATCAA+CGG - chr5.2:7607728-7607747 None:intergenic 40.0%
TGAAAGACATAAGGTCAGCA+CGG - chr5.2:7607353-7607372 None:intergenic 40.0%
! TACAACCCTTTCTAAGGACA+CGG - chr5.2:7607239-7607258 None:intergenic 40.0%
!! TCTTTGTTGTTTGAAGCGTG+TGG + chr5.2:7607556-7607575 MS.gene09579:CDS 40.0%
!!! TGCTGAAGTTTTGAAGCTTC+CGG + chr5.2:7607837-7607856 MS.gene09579:CDS 40.0%
AAACCCTTACCGGAGTTTCT+CGG + chr5.2:7607682-7607701 MS.gene09579:CDS 45.0%
AAACCGAGAAACTCCGGTAA+GGG - chr5.2:7607688-7607707 None:intergenic 45.0%
AAAGTCCGGTTCATGAGTTC+TGG + chr5.2:7607793-7607812 MS.gene09579:CDS 45.0%
AACACCGTCGATGAATTCTG+AGG + chr5.2:7607939-7607958 MS.gene09579:CDS 45.0%
AACTCATGAACCGGACTTTC+TGG - chr5.2:7607792-7607811 None:intergenic 45.0%
AGATTAAGATCAGTCGTCGC+CGG - chr5.2:7607859-7607878 None:intergenic 45.0%
CGACGACTGATCTTAATCTC+CGG + chr5.2:7607860-7607879 MS.gene09579:CDS 45.0%
GAAACTCCGGTAAGGGTTTA+AGG - chr5.2:7607681-7607700 None:intergenic 45.0%
GGAGATAACTACGTTCACGA+CGG + chr5.2:7608003-7608022 MS.gene09579:CDS 45.0%
TAAACCGAGAAACTCCGGTA+AGG - chr5.2:7607689-7607708 None:intergenic 45.0%
TACCACCAGAACTCATGAAC+CGG - chr5.2:7607801-7607820 None:intergenic 45.0%
TTCAGATGAGTCATCAACGG+TGG - chr5.2:7607725-7607744 None:intergenic 45.0%
! GACTTTTGTGGACAGGAAAC+TGG + chr5.2:7607611-7607630 MS.gene09579:CDS 45.0%
! TTCTGAGGAATCTGTGACGA+CGG + chr5.2:7607954-7607973 MS.gene09579:CDS 45.0%
!! ACCGTTGATGACTCATCTGA+AGG + chr5.2:7607724-7607743 MS.gene09579:CDS 45.0%
!! ATGAACCGGACTTTCTGGTT+CGG - chr5.2:7607787-7607806 None:intergenic 45.0%
AAACTGGCACGTGTGTCAAG+CGG + chr5.2:7607627-7607646 MS.gene09579:CDS 50.0%
AAGTCCGACAGCACCGTTAA+CGG - chr5.2:7607596-7607615 None:intergenic 50.0%
AATTTCCCTCCGGAAACCGT+TGG - chr5.2:7607908-7607927 None:intergenic 50.0%
ACGTGCTTGGAAAGTGGGAT+CGG + chr5.2:7607979-7607998 MS.gene09579:CDS 50.0%
AGAACCGTGAACCCCGTTAA+CGG + chr5.2:7607580-7607599 MS.gene09579:CDS 50.0%
ATCGATACCCCTGAAGCTCA+AGG + chr5.2:7607294-7607313 MS.gene09579:CDS 50.0%
CGGAACCCTTAAACCCTTAC+CGG + chr5.2:7607672-7607691 MS.gene09579:CDS 50.0%
GAATTCATCGACGGTGTTCC+CGG - chr5.2:7607937-7607956 None:intergenic 50.0%
GAGATCCGAACCAGAAAGTC+CGG + chr5.2:7607779-7607798 MS.gene09579:CDS 50.0%
GCGTCTAAACCGAGAAACTC+CGG - chr5.2:7607694-7607713 None:intergenic 50.0%
GGTTGCCGTGTCCTTAGAAA+GGG + chr5.2:7607231-7607250 MS.gene09579:CDS 50.0%
TACCAGGTCTCTGTGGTGAA+GGG - chr5.2:7607386-7607405 None:intergenic 50.0%
TGGCATGACCTTGAGCTTCA+GGG - chr5.2:7607305-7607324 None:intergenic 50.0%
TTACCAGGTCTCTGTGGTGA+AGG - chr5.2:7607387-7607406 None:intergenic 50.0%
!! GGACTTTCTGGTTCGGATCT+CGG - chr5.2:7607780-7607799 None:intergenic 50.0%
ACGCGTTCTGTGGCGTTAAC+CGG - chr5.2:7607882-7607901 None:intergenic 55.0%
ACGGTGACGTGCTTGGAAAG+TGG + chr5.2:7607973-7607992 MS.gene09579:CDS 55.0%
AGCACCGTTAACGGGGTTCA+CGG - chr5.2:7607587-7607606 None:intergenic 55.0%
AGTCCGACAGCACCGTTAAC+GGG - chr5.2:7607595-7607614 None:intergenic 55.0%
CGGTGACGTGCTTGGAAAGT+GGG + chr5.2:7607974-7607993 MS.gene09579:CDS 55.0%
CGGTTGCCGTGTCCTTAGAA+AGG + chr5.2:7607230-7607249 MS.gene09579:CDS 55.0%
GGCATGACCTTGAGCTTCAG+GGG - chr5.2:7607304-7607323 None:intergenic 55.0%
GGTGGTAAACGCAAGCGATC+AGG + chr5.2:7607814-7607833 MS.gene09579:CDS 55.0%
GTCAGCACGGCCGAAAAACT+TGG - chr5.2:7607340-7607359 None:intergenic 55.0%
GTGGCATGACCTTGAGCTTC+AGG - chr5.2:7607306-7607325 None:intergenic 55.0%
GTGTCAAGCGGCGGTTGAAA+CGG + chr5.2:7607639-7607658 MS.gene09579:CDS 55.0%
GTTGACCAACGGTTTCCGGA+GGG + chr5.2:7607900-7607919 MS.gene09579:CDS 55.0%
GTTTCCGGAGGGAAATTCGC+CGG + chr5.2:7607911-7607930 MS.gene09579:CDS 55.0%
TGCTTGGAAAGTGGGATCGG+AGG + chr5.2:7607982-7608001 MS.gene09579:CDS 55.0%
! ACGCGTTGACCAACGGTTTC+CGG + chr5.2:7607896-7607915 MS.gene09579:CDS 55.0%
! CCACAGAACGCGTTGACCAA+CGG + chr5.2:7607889-7607908 MS.gene09579:CDS 55.0%
! GCTGTCGGACTTTTGTGGAC+AGG + chr5.2:7607604-7607623 MS.gene09579:CDS 55.0%
! GTCCGGTTCATGAGTTCTGG+TGG + chr5.2:7607796-7607815 MS.gene09579:CDS 55.0%
!! AACCCCGTTAACGGTGCTGT+CGG + chr5.2:7607589-7607608 MS.gene09579:CDS 55.0%
!!! ACGGTGCTGTCGGACTTTTG+TGG + chr5.2:7607599-7607618 MS.gene09579:CDS 55.0%
AAGTCACGTGCAACGACGCG+CGG + chr5.2:7607749-7607768 MS.gene09579:CDS 60.0%
CCGTTGGTCAACGCGTTCTG+TGG - chr5.2:7607892-7607911 None:intergenic 60.0%
CGTTGACCAACGGTTTCCGG+AGG + chr5.2:7607899-7607918 MS.gene09579:CDS 60.0%
GTCCCTTCACCACAGAGACC+TGG + chr5.2:7607381-7607400 MS.gene09579:CDS 60.0%
GTCCGACAGCACCGTTAACG+GGG - chr5.2:7607594-7607613 None:intergenic 60.0%
TGTGACGACGGTGACGTGCT+TGG + chr5.2:7607966-7607985 MS.gene09579:CDS 60.0%
! GTTGAAACGGTGCTCGGTGG+CGG + chr5.2:7607652-7607671 MS.gene09579:CDS 60.0%
CTGGCACGTGTGTCAAGCGG+CGG + chr5.2:7607630-7607649 MS.gene09579:CDS 65.0%
GCGGCGGTTGAAACGGTGCT+CGG + chr5.2:7607646-7607665 MS.gene09579:CDS 65.0%
GCGGTTGAAACGGTGCTCGG+TGG + chr5.2:7607649-7607668 MS.gene09579:CDS 65.0%
TCATCGACGGTGTTCCCGGC+CGG - chr5.2:7607933-7607952 None:intergenic 65.0%
CCGGAGGGAAATTCGCCGGC+CGG + chr5.2:7607915-7607934 MS.gene09579:CDS 70.0%
CCGGCCGGCGAATTTCCCTC+CGG - chr5.2:7607918-7607937 None:intergenic 70.0%
CGGAGGGAAATTCGCCGGCC+GGG + chr5.2:7607916-7607935 MS.gene09579:CDS 70.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 7607219 7608056 7607219 ID=MS.gene09579
chr5.2 mRNA 7607219 7608056 7607219 ID=MS.gene09579.t1;Parent=MS.gene09579
chr5.2 exon 7607219 7607402 7607219 ID=MS.gene09579.t1.exon1;Parent=MS.gene09579.t1
chr5.2 CDS 7607219 7607402 7607219 ID=cds.MS.gene09579.t1;Parent=MS.gene09579.t1
chr5.2 exon 7607545 7608056 7607545 ID=MS.gene09579.t1.exon2;Parent=MS.gene09579.t1
chr5.2 CDS 7607545 7608056 7607545 ID=cds.MS.gene09579.t1;Parent=MS.gene09579.t1
Gene Sequence

>MS.gene09579

ATGAGTTGCAACGGTTGCCGTGTCCTTAGAAAGGGTTGTAGTGAGTCATGTATATTACGCCCTTGTATTCAATGGATCGATACCCCTGAAGCTCAAGGTCATGCCACTGTCTTTGTAGCCAAGTTTTTCGGCCGTGCTGACCTTATGTCTTTCATTTCTAACGTCCCTTCACCACAGAGACCTGGTAAATAATCTTTATCTTCTTTTTTTTACTCCACAGTTACACTTTCACTCTAAGTAGTTTGTCACATGATTCTGACAAACTCACTTTGATATAAGCTTTTAGATGAACTAAACTAACTAAGGTTTCTTTTTATGTTATGCAGCTTTGTTTCAATCTTTGTTGTTTGAAGCGTGTGGAAGAACCGTGAACCCCGTTAACGGTGCTGTCGGACTTTTGTGGACAGGAAACTGGCACGTGTGTCAAGCGGCGGTTGAAACGGTGCTCGGTGGCGGAACCCTTAAACCCTTACCGGAGTTTCTCGGTTTAGACGCTCTTACTTCCACCGTTGATGACTCATCTGAAGGAGAAGTCACGTGCAACGACGCGCGGAAGATCCGAGATCCGAACCAGAAAGTCCGGTTCATGAGTTCTGGTGGTAAACGCAAGCGATCAGGTGCTGAAGTTTTGAAGCTTCCGGCGACGACTGATCTTAATCTCCGGTTAACGCCACAGAACGCGTTGACCAACGGTTTCCGGAGGGAAATTCGCCGGCCGGGAACACCGTCGATGAATTCTGAGGAATCTGTGACGACGGTGACGTGCTTGGAAAGTGGGATCGGAGGAGATAACTACGTTCACGACGGAGACCGCAAAGTTCTCAACCTTTTTGTTTGA

Protein sequence

>MS.gene09579.t1

MSCNGCRVLRKGCSESCILRPCIQWIDTPEAQGHATVFVAKFFGRADLMSFISNVPSPQRPALFQSLLFEACGRTVNPVNGAVGLLWTGNWHVCQAAVETVLGGGTLKPLPEFLGLDALTSTVDDSSEGEVTCNDARKIRDPNQKVRFMSSGGKRKRSGAEVLKLPATTDLNLRLTPQNALTNGFRREIRRPGTPSMNSEESVTTVTCLESGIGGDNYVHDGDRKVLNLFV